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Spermatogenesis depletion in diploid and triploid yellowtail tetra (Astyanax altiparanae) for germ cell transplantation. 二倍体和三倍体黄尾四鱼生殖细胞移植的精子发生缺失。

IF 1.4 4区生物学

Zygote Pub Date : 2026-04-16 DOI: 10.1017/S0967199426100380

Lucia Suarez Lopez, Rafaela Manchin Bertolini, Paulo Monzani, Gabriella Braga Carvalho, Diógenes Henrique de Siqueira-Silva, Jose Augusto Senhorini, Norberto Vianna, George Shigueki Yasui

{"title":"Spermatogenesis depletion in diploid and triploid yellowtail tetra (Astyanax altiparanae) for germ cell transplantation.","authors":"Lucia Suarez Lopez, Rafaela Manchin Bertolini, Paulo Monzani, Gabriella Braga Carvalho, Diógenes Henrique de Siqueira-Silva, Jose Augusto Senhorini, Norberto Vianna, George Shigueki Yasui","doi":"10.1017/S0967199426100380","DOIUrl":"https://doi.org/10.1017/S0967199426100380","url":null,"abstract":"The piracanjuba (Brycon orbignyanus) is a species threatened with extinction due to human activities, highlighting the need for genebanking and alternative propagation methods for its conservation. The objective of this study was to obtain a completely sterile yellowtail tetra (Astyanax altiparanae) and to transplant the stem spermatogonia (SSCs) of B. orbignyanus. Complete depletion of spermatogenesis in adult diploid males was successfully achieved through five applications of busulfan, using dosages of 15 mg kg-1 and 40 mg kg-1 at a high temperature of 35°C. One month after completing the busulfan treatment, spermatogenesis returned, occurring more rapidly in the 15 mg kg-1 treatment, with spermatozoa visible in the testicular lumen. In the treatment with 40 mg kg-1, only SSCs and some spermatid cysts were observed. Depletion of spermatogenesis in triploid juveniles was observed after two applications of busulfan (50 mg kg-1). One month after the end of the treatment, only a few SSCs cells were observed in the testes. The B. orbignyanus SSCs were isolated using a Percoll density gradient and identified by alkaline phosphatase activity, resulting in a cell suspension of 2.6 × 106 SSCs/mL with 93% cell viability. The cells were labeled with PKH26 and transplanted via the urogenital papilla into recipients (triploid juvenile males) previously treated with busulfan. PKH26-positive cells were visualized up to 80 days after transplantation. The combination of two sterilization methods appears to be more effective for cell transplantation after the endogenous germ cells have been eliminated from the recipient testes of triploid juveniles.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-14"},"PeriodicalIF":1.4,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal induction ensures the production of high-quality semen in fish compared to the presence of females from different production systems. 与来自不同生产系统的雌性相比，激素诱导可以确保鱼类产生高质量的精液。

IF 1.4 4区生物学

Zygote Pub Date : 2026-04-13 DOI: 10.1017/S0967199426100367

Antoni Manoel de Souza Cruz, Gabriel Pereira de Sousa, Laura Andrade de Moura, Eduardo Jorge Fernandes, Vinicius Rogério Leite, Edicarlos Pereira de Sousa, Raimundo Nonato Costa Ferreira, Cleonice Cristina Hilbig, George Shigueki Yasui, Nivaldo Ferreira do Nascimento

{"title":"Hormonal induction ensures the production of high-quality semen in fish compared to the presence of females from different production systems.","authors":"Antoni Manoel de Souza Cruz, Gabriel Pereira de Sousa, Laura Andrade de Moura, Eduardo Jorge Fernandes, Vinicius Rogério Leite, Edicarlos Pereira de Sousa, Raimundo Nonato Costa Ferreira, Cleonice Cristina Hilbig, George Shigueki Yasui, Nivaldo Ferreira do Nascimento","doi":"10.1017/S0967199426100367","DOIUrl":"https://doi.org/10.1017/S0967199426100367","url":null,"abstract":"This study investigated how hormonal induction, female presence, and production system affect sperm quality in Astyanax lacustris across three experiments. In Experiment 1, males and females were kept together in the same recirculating aquaculture system (RAS) before testing. Hormonal induction consistently boosted initial motility and prolonged sperm activity, while female presence offered only a modest benefit to non-induced males and no measurable effect when males were induced. Sperm concentration remained similar across treatments. Experiment 2 evaluated the same factors using broodstock originating from different rearing environments. When males and females came from separate RAS units, hormonal induction again sustained higher motility, and induced males paired with females showed higher sperm concentration than some non-induced groups. In the RAS-biofloc technology (BFT) pairing, hormonal induction maintained motility regardless of female presence; however, in the absence of hormonal induction, both sperm motility and concentration were modulated by the rearing system of females, with non-induced males paired with BFT females exhibiting lower reproductive performance. Overall, hormonal induction proved to be the most reliable strategy for ensuring high semen quality in A. lacustris. Nonetheless, when induction was not applied, male reproductive performance became more sensitive to female origin and rearing environment, highlighting the importance of broodstock compatibility and production system history in reproductive management protocols.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-6"},"PeriodicalIF":1.4,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147677161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene expression dynamics of TLR2 in twin- and single-bearing goats: a step toward molecular reproductive profiling. TLR2基因在双胎和单胎山羊中的表达动态：迈向分子生殖谱的一步。

IF 1.4 4区生物学

Zygote Pub Date : 2026-02-01 Epub Date: 2026-03-31 DOI: 10.1017/S096719942610032X

Ankit Magotra, Yogesh C Bangar, Sandeep Kumar, Devesh Kumar Yadav, Anil Bhat, Rohit Kumar

{"title":"Gene expression dynamics of TLR2 in twin- and single-bearing goats: a step toward molecular reproductive profiling.","authors":"Ankit Magotra, Yogesh C Bangar, Sandeep Kumar, Devesh Kumar Yadav, Anil Bhat, Rohit Kumar","doi":"10.1017/S096719942610032X","DOIUrl":"10.1017/S096719942610032X","url":null,"abstract":"This study investigated the expression profile of the Toll-like receptor 2 (TLR2) gene in goats across three reproductive groups - twin-bearing (n = 12), single-bearing (n = 10) and non-pregnant (n = 8) - using quantitative real-time PCR (qRT-PCR). Descriptive statistics revealed that twin-bearing goats were significantly heavier at service and kidding, with higher litter weights compared to other groups (p < 0.05). No breed-wise differences were observed, allowing pooled analysis for gene expression. Melt curve and amplification plot analyses confirmed primer specificity and efficient amplification. TLR2 expression was significantly upregulated in twin-bearing goats (ΔCt = -2.14 ± 0.89), with a mean 4.5-fold increase (p < 0.001) compared to non-pregnant controls, suggesting enhanced immune activation. Single-bearing goats showed negligible and non-significant changes in expression (ΔCt = -0.01 ± 1.45; p = 0.984). Statistical comparisons showed a significant difference in TLR2 expression between twin- and single-bearing groups (mean difference: 3.39; p < 0.001). These results reveals a significant association of twin pregnancies with elevated expression level of TLR2 gene in resource population. While the functional implications require further investigation, this upregulation may be associated with immune adaptations to increased gestational load. Overall, this study identifies TLR2 as a candidate gene expression marker strongly correlated with twin pregnancy in goats. Further validation through longitudinal studies on larger cohorts and investigation of tissue-specific expression is necessary to assess its potential predictive value for reproductive performance in small ruminants.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"12-19"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147582242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

kdm4aa knockout disturbs early embryonic development in Zebrafish via downregulating rbm46. 敲除kdm4aa通过下调rbm46干扰斑马鱼早期胚胎发育。

IF 1.4 4区生物学

Zygote Pub Date : 2026-02-01 Epub Date: 2026-03-09 DOI: 10.1017/S0967199425100257

Hui Jiang, Xinqing Cai, Rui Deng, Jiamin Wang, Hongkuan Song, Weiran Chai, Bingshe Han, Junfang Zhang

{"title":"kdm4aa knockout disturbs early embryonic development in Zebrafish via downregulating rbm46.","authors":"Hui Jiang, Xinqing Cai, Rui Deng, Jiamin Wang, Hongkuan Song, Weiran Chai, Bingshe Han, Junfang Zhang","doi":"10.1017/S0967199425100257","DOIUrl":"10.1017/S0967199425100257","url":null,"abstract":"Histone lysine-specific demethylase 4A (KDM4A) plays a critical role in the embryonic development of mammals such as mouse and goat, however its function in zebrafish (Danio rerio) embryogenesis remains poorly understood, due to the existence of two kdm4a paralogs (kdm4aa and kdm4ab) in zebrafish. The current study revealed that kdm4aa-/- embryos exhibited dramatically increased mortality during gastrulation. RT-qPCR showed that RNA-binding motif protein 46 (rbm46) was downregulated after kdm4aa knockout. CUT&Tag-qPCR revealed that kdm4aa knockout significantly decreased H3K4me3, while increasing H3K9me3 and H3K36me3 at rbm46 promoter. kdm4aa-/- embryos displayed elevated reactive oxygen species (ROS) and reduced adenosine triphosphate (ATP). And rbm46 mRNA injection alleviated ROS accumulation and increased ATP level, thereby rescuing the lethal phenotype in kdm4aa-/- embryos. Our findings demonstrate that kdm4aa knockout disturbs zebrafish embryogenesis by suppressing rbm46 mRNA expression.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"29-39"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro fertilization outcomes in young women and their association with male and female infertility aetiology. 年轻女性体外受精结果及其与男性和女性不孕症病因的关系。

IF 1.4 4区生物学

Zygote Pub Date : 2026-02-01 Epub Date: 2026-03-23 DOI: 10.1017/S0967199426100306

Gilad Karavani, Hiila Bauch, Natali Schachter-Safrai, Tal Imbar, Assaf Ban-Meir

{"title":"In vitro fertilization outcomes in young women and their association with male and female infertility aetiology.","authors":"Gilad Karavani, Hiila Bauch, Natali Schachter-Safrai, Tal Imbar, Assaf Ban-Meir","doi":"10.1017/S0967199426100306","DOIUrl":"10.1017/S0967199426100306","url":null,"abstract":"Infertility aetiology has been shown to be related to reproductive outcomes in the wide reproductive age range. The aim of this study was to examine young women in vitro fertilization (IVF) cycle outcomes according to age and infertility cause. This retrospective cohort study was comprised of 305 couples with a female partner aged 17-25 years with either male or female infertility that underwent a fresh IVF cycle between 2003 and 2020. Comparison according to age groups 17-22 years (n = 117) and 23-25 years (n = 188) showed similar outcomes, except for higher rates of 3 pronuclei (PN) embryos in the younger group. However, allocation to groups by infertility aetiology - male factor (MF) (n = 241) vs. female factor (FF) (n = 64) demonstrated lower fertilization rate in the FF vs. MF group (58% vs. 63%, p = 0.049) and higher percent of 1PN and 3PN embryos (0.7% vs. 0.2% and 0.7% vs. 0.1%, p < 0.001 for both). Chemical pregnancy and live-birth rate were significantly higher in the MF vs. FF group (48.9% vs. 29.5%, p = 0.02 and 41.9% vs. 11.0%, p = 0.038). The only parameter associated with live birth rate in a logistic regression analysis was male infertility aetiology (OR = 2.17, p = 0.038). Young couples undergoing IVF cycles due to female factor infertility have poorer reproductive outcomes compared to male factor-related infertility and higher rates of 1PN and 3PN embryos.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"4-11"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147499974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embryo morphokinetics and PGT-A outcomes in recurrent pregnancy loss cases. 反复流产病例的胚胎形态动力学和PGT-A结果。

IF 1.4 4区生物学

Zygote Pub Date : 2026-02-01 Epub Date: 2026-04-07 DOI: 10.1017/S0967199426100355

Tutku Melis Aygun, Gulcin Ozkara, Ipek Nur Balin Duzguner, Caroline Pirkevi Cetinkaya, Hakan Kadir Yelke, Yesim Kumtepe, Semra Yildiz, Serkan Selimoglu, Semra Kahraman, Tulay Irez

{"title":"Embryo morphokinetics and PGT-A outcomes in recurrent pregnancy loss cases.","authors":"Tutku Melis Aygun, Gulcin Ozkara, Ipek Nur Balin Duzguner, Caroline Pirkevi Cetinkaya, Hakan Kadir Yelke, Yesim Kumtepe, Semra Yildiz, Serkan Selimoglu, Semra Kahraman, Tulay Irez","doi":"10.1017/S0967199426100355","DOIUrl":"10.1017/S0967199426100355","url":null,"abstract":"Recurrent pregnancy loss (RPL) is a clinically important condition in women undergoing assisted reproductive technologies (ART). This study evaluated embryo morphology and morphokinetic parameters using time-lapse monitoring (TLM) and assessed embryo ploidy by preimplantation genetic testing for aneuploidy (PGT-A) in women with RPL compared with unexplained infertility (UEI) controls. A total of 190 patients (100 RPL, 90 UEI) and 1169 embryos (634 RPL, 535 UEI) cultured under TLM were analyzed. Clinical characteristics, embryo morphology, morphokinetics and ploidy status were compared between groups, and logistic regression was used to identify predictors of aneuploidy. The euploidy rate was significantly lower in the RPL group than in controls (43.5% vs. 52.3%, p = 0.018). Group-wise analysis of all embryos revealed differences in selected morphokinetic parameters (t9 and tSC; p < 0.05). However, morphokinetic timing of euploid embryos did not differ between groups, suggesting that accelerated development alone was not associated with chromosomal abnormality. Embryo morphology was the strongest predictor of aneuploidy in both cohorts. Notably, direct uneven cleavage was associated with a 3.4-fold increased risk of aneuploidy specifically in the RPL group. Although embryos from RPL patients showed relatively faster developmental kinetics, morphokinetic speed alone did not predict chromosomal competence. Instead, embryo quality remained the key determinant of aneuploidy, while the association between direct cleavage and aneuploidy highlights the potential clinical value of TLM when combined with PGT-A. These findings support a complementary role for TLM in embryo assessment in RPL patients.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"20-28"},"PeriodicalIF":1.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147628766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid supplementation in donor cows (Bos taurus) improves embryo quality. 供体奶牛（Bos taurus）补充脂质可提高胚胎质量。

IF 1.4 4区生物学

Zygote Pub Date : 2026-02-01 Epub Date: 2026-03-27 DOI: 10.1017/S0967199426100331

Christopher Junior Tavares Cardoso, Ana Caroline Bini de Lima, Mirela Brochado Souza-Cáceres, Marcus Vinicius Moraes de Oliveira, Eriklis Nogueira, Fabiana de Andrade Melo Sterza

引用次数: 0

Ovarian stimulation with r-hFSH:r-hLH: a descriptive analysis of embryonic morphokinetic data. 用r-hFSH:r-hLH刺激卵巢：胚胎形态动力学数据的描述性分析。

IF 1.4 4区生物学

Zygote Pub Date : 2025-12-01 Epub Date: 2026-02-02 DOI: 10.1017/S0967199426100276

Edson Borges, Daniela Paes Almeida Ferreira Braga, Assumpto Iaconelli, Amanda Setti

{"title":"Ovarian stimulation with r-hFSH:r-hLH: a descriptive analysis of embryonic morphokinetic data.","authors":"Edson Borges, Daniela Paes Almeida Ferreira Braga, Assumpto Iaconelli, Amanda Setti","doi":"10.1017/S0967199426100276","DOIUrl":"10.1017/S0967199426100276","url":null,"abstract":"The objective of this study was to compare embryonic morphokinetic parameters and clinical outcomes of two controlled ovarian stimulation (COS) protocols in women of advanced maternal age (AMA): Pergoveris (r-hFSH:r-hLH, 2:1 ratio) versus r-hFSH monotherapy (GONAL-f). This retrospective, non-interventional descriptive study was conducted at a private university-affiliated IVF center and included 136 ICSI cycles performed in AMA patients between March 2019 and May 2020. Patients were grouped by COS protocol (GONAL-f, n = 64; Pergoveris, n = 72), and embryo morphokinetics and ICSI outcomes were extracted from the clinic's database; the main outcome was time to complete blastulation (tB, hours). Embryos from the Pergoveris group reached tB earlier than those from the GONAL-f group (mean 109.3 h vs 112.6 h), and all morphokinetic milestones occurred sooner with Pergoveris, alongside lower multinucleation rates at the 2-cell and 4-cell stages. Although blastocyst development was higher in the GONAL-f group, Pergoveris was associated with higher oocyte yield and maturity, higher implantation, clinical and ongoing pregnancy rates, and lower miscarriage and OHSS rates; Pergoveris cycles also required higher gonadotropin doses and longer stimulation. Overall, embryos from the r-hFSH:r-hLH group exhibited faster morphokinetic timings and improved implantation and pregnancy outcomes, and prospective studies are warranted to confirm these observations.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"295-307"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding early development: a detailed study of Clarias dussumieri (Valenciennes, 1840), a near-threatened catfish of the Western Ghats. 了解早期发展：对西高塞山脉一种濒临灭绝的鲶鱼Clarias dussumieri （Valenciennes, 1840）的详细研究。

IF 1.4 4区生物学

Zygote Pub Date : 2025-12-01 Epub Date: 2026-02-18 DOI: 10.1017/S0967199426100288

Sarath Varghese, Charan Ravi, Neethu Z, Abhilash C P, Saikrishnan K R, Chandana B L, Ajith Kumar T T, Uttam Kumar Sarkar, Basheer V S

{"title":"Understanding early development: a detailed study of Clarias dussumieri (Valenciennes, 1840), a near-threatened catfish of the Western Ghats.","authors":"Sarath Varghese, Charan Ravi, Neethu Z, Abhilash C P, Saikrishnan K R, Chandana B L, Ajith Kumar T T, Uttam Kumar Sarkar, Basheer V S","doi":"10.1017/S0967199426100288","DOIUrl":"10.1017/S0967199426100288","url":null,"abstract":"The study documents the embryonic and larval development of Clarias dussumieri, a Near-Threatened, cultivable, and highly valued food fish endemic to the Western Ghats. Induced breeding facilitated detailed observation of developmental stages using light microscopy. Unfertilized eggs measured 1.5 ± 0.02 mm in diameter and enlarged to 1.7 ± 0.10 mm after fertilization (∼13% increase). The first cleavage occurred at 00:36 ± 00:02 h, reaching 64 cells by 01:35 ± 00:06 h. Epiboly progressed from 30% at 03:20 ± 00:10 h to 50% at 04:30 ± 00:05 h and 90% at 05:50 ± 00:10 h; neurulation was evident by 07:40 ± 01:10 h and somites by 11:08 ± 01:26 h. The first heartbeat appeared during the pharyngula period (17:25 ± 01:30 h), and hatching occurred at 25:30 ± 01:30 h at 26-27°C. Hatched larvae were 4.2 ± 0.02 mm total length, observed few melanophores by 12 h, conspicuous eye pigmentation by 24 h, and body pigmentation spreading by day 2 (5.90 ± 0.04 mm).The yolk sac was fully absorbed by 72 ± 2.50 h post-hatching, marking the onset of exogenous feeding. By day 4 (pre-flexion), larvae reached 7.1 ± 0.02 mm; initial notochord flexion began around day 7 and post-flexion from day 10, with juvenile-like morphology evident by day 25 (15.27-17.80 mm). The standardized timelines and measurements provide a baseline for hatchery practice and conservation aquaculture of C. dussumieri, supporting protocol refinement for improving survival and growth under controlled conditions.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"323-336"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146214437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the impacts of COVID-19 infection and vaccination on the outcomes of assisted reproductive technology (ART) cycles. 评估COVID-19感染和疫苗接种对辅助生殖技术（ART）周期结果的影响

IF 1.4 4区生物学

Zygote Pub Date : 2025-12-01 Epub Date: 2025-12-22 DOI: 10.1017/S0967199425100245

Farzaneh Shamlou Ahmadi, Ashraf Moini, Arezoo Arabipoor, Zahra Zolfaghari, Bita Ebrahimi, Firouzeh Ghaffari

{"title":"Evaluation of the impacts of COVID-19 infection and vaccination on the outcomes of assisted reproductive technology (ART) cycles.","authors":"Farzaneh Shamlou Ahmadi, Ashraf Moini, Arezoo Arabipoor, Zahra Zolfaghari, Bita Ebrahimi, Firouzeh Ghaffari","doi":"10.1017/S0967199425100245","DOIUrl":"10.1017/S0967199425100245","url":null,"abstract":"This study aimed to evaluate the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and inactivated virus vaccination on intracytoplasmic sperm injection (ICSI) outcomes in infertile couples. A retrospective case-control study was conducted at the Royan Institute from August 2020 to March 2022. The study included 90 couples in the COVID-19 infection phase and 31 in the vaccination phase. A total of 30 infected but unvaccinated couples were compared to a control group of 60 couples with no COVID-19 infection or vaccination history. Additionally, 31 couples underwent treatment before and after receiving the Sinopharm inactivated vaccine. Key variables analysed included sperm parameters (concentration, motility, progressive motility and morphology), ovarian parameters (antral follicle count, oocyte retrieval), embryological outcomes and pregnancy outcomes. SARS-CoV-2 infection significantly reduced sperm motility (P = 0.02) and progressive motility (P = 0.01) compared to controls. Sperm concentration and morphology showed non-significant declines. Post-vaccination analysis revealed similar but statistically insignificant changes in sperm parameters. Ovarian stimulation parameters and embryological outcomes remained unaffected by both infection and vaccination. Although biochemical, clinical pregnancy and live birth rates were lower among the infected group, these differences did not reach statistical significance (p = 0.16, 0.08 and 0.09). SARS-CoV-2 infection has been associated with impaired sperm progressive motility, which may negatively influence ICSI outcomes. In contrast, vaccination with an inactivated virus does not appear to impact fertility outcomes. These findings provide crucial guidance for physicians and infertile couples managing treatments during and after the pandemic, suggesting the need for extended recovery periods before ART procedures following COVID-19 infection.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"285-294"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145805773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0