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Supplementation with specific branched-chain amino acids (BCAAs) affects mouse sperm parameters and testicular apoptotic gene expression. 补充特定支链氨基酸（BCAAs）影响小鼠精子参数和睾丸凋亡基因表达。

IF 1.5 4区生物学

Zygote Pub Date : 2025-05-13 DOI: 10.1017/S0967199425000073

Roya Kamali, Leila Rashki Ghaleno, Amin Izadi, Zahra-Sadat Ghasemi, Vahid Esmaeili, Nima Eila, Adel Pezeshki, Joël R Drevet, Abdolhossein Shahverdi, AliReza Alizadeh Moghadam Masouleh

{"title":"Supplementation with specific branched-chain amino acids (BCAAs) affects mouse sperm parameters and testicular apoptotic gene expression.","authors":"Roya Kamali, Leila Rashki Ghaleno, Amin Izadi, Zahra-Sadat Ghasemi, Vahid Esmaeili, Nima Eila, Adel Pezeshki, Joël R Drevet, Abdolhossein Shahverdi, AliReza Alizadeh Moghadam Masouleh","doi":"10.1017/S0967199425000073","DOIUrl":"https://doi.org/10.1017/S0967199425000073","url":null,"abstract":"In Western diets, high consumption of meat and dairy products, known to be rich in branched-chain amino acids (BCAAs), including leucine (Leu), isoleucine (Ile), and valine (Val), as well as BCAAs supplementation itself, may have unforeseen consequences on sperm quality. In addition, bodybuilders are increasingly resorting to BCAA supplementation to build-up their muscle mass. This study aimed to assess the effect of dietary BCAAs, provided alone or in combination, on semen parameters, apoptotic gene expression, and blood amino acid (AA) profiles. To address this question and determine whether these different BCAAs have a distinct impact on sperm quality and testicular homeostasis, fifty NMRI mature male mice were exposed or not to BCAAs supplementations (control diet: CTR; CTR + Leu supplementation; CTR + Ile supplementation; CTR + Val supplementation; CTR + all three BCAA supplementation). Only valine supplementation resulted in a significant decrease in sperm concentration and viability. In addition, only valine supplementation was associated with a dramatic increase in sperm immotility. The Bax/Bcl2 ratio, an indicator of apoptosis, was found to be significantly higher in the testes of BCAA-supplemented animals when compared with the other groups. Caspase3 expression was also significantly higher in the testes of BCAA-supplemented and Val-supplemented animals. There were no significant differences in plasma AA profiles between groups. Thus, amongst BCAAs, valine supplementation appears to carry the greatest effect on sperm functional parameters and testicular apoptotic status.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-9"},"PeriodicalIF":1.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sesquizygotic twinning: a unique twinning type rather than mechanism. 同卵双胞胎：一种独特的双胞胎类型，而不是机制。

IF 1.5 4区生物学

Zygote Pub Date : 2025-05-13 DOI: 10.1017/S0967199425000097

Yuge Tan

引用次数: 0

The role of sperm protein in mammal fertilization: insights into gamete adhesion, membrane fusion and oocyte activation. 精子蛋白在哺乳动物受精中的作用：对配子粘附、膜融合和卵母细胞激活的见解。

IF 1.5 4区生物学

Zygote Pub Date : 2025-05-13 DOI: 10.1017/S0967199425000085

Kaiyue Hu, Bo Dong, Yugang Wang, Xiangrui Meng

{"title":"The role of sperm protein in mammal fertilization: insights into gamete adhesion, membrane fusion and oocyte activation.","authors":"Kaiyue Hu, Bo Dong, Yugang Wang, Xiangrui Meng","doi":"10.1017/S0967199425000085","DOIUrl":"https://doi.org/10.1017/S0967199425000085","url":null,"abstract":"Globally, numerous infertile couples have been assisted by extensive research on mammalian fertilization and the rapid development of Assisted Reproductive Technology (ART). However, 5%-15% of the couples that are selected for in vitro fertilization (IVF) experience a total fertilization failure (TFF), where no zygotes develop despite oocytes and semen parameters appear to be normal. Notably, an essential early event in fertilization is the binding of spermatozoa to the oocyte's external envelope, which followed by the spermatozoa-oocyte fusion. Meanwhile, oocyte activation is a crucial cellular process necessary to block polyspermy and start the development of the zygote. Improper membrane fusion of gametes has been demonstrated to be one of the mechanisms of TFF. Moreover, considering the large amount of research on sperm proteins in recent years, thus in this review, we characterize the role and molecular mechanisms of sperm proteins in the three key processes of gamete adhesion and fusion and oocyte activation, which would provide a comprehensive understanding of the role of sperm proteins in fertilization in mammals and a favourable reference for future studies in assisted reproduction due to FF.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-11"},"PeriodicalIF":1.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case report of a successful pregnancy after intracytoplasmic sperm injection when all oocytes contained abnormal inclusions in the perivitelline space. 一例卵母细胞卵周间隙异常包涵体，经卵浆内单精子注射成功妊娠的病例报告。

IF 1.5 4区生物学

Zygote Pub Date : 2025-03-21 DOI: 10.1017/S096719942500005X

Juan Peng, Wenda Zou, Liyu Zhu, Xinlin Guo, Juan Zhang, Hui Li

{"title":"A case report of a successful pregnancy after intracytoplasmic sperm injection when all oocytes contained abnormal inclusions in the perivitelline space.","authors":"Juan Peng, Wenda Zou, Liyu Zhu, Xinlin Guo, Juan Zhang, Hui Li","doi":"10.1017/S096719942500005X","DOIUrl":"https://doi.org/10.1017/S096719942500005X","url":null,"abstract":"Background: The relationship between oocyte morphology and developmental potential has been a hot research topic in assisted reproductive technology (ART). Whether inclusions in the perivitelline space (PVS) affect ART outcomes remains controversial.Case presentation: We present a case report of a 34-year-old G3P1A2 woman who sought ART treatment because of sequelae of pelvic disease. As her husband had severe oligospermia due to the stress on the day of oocyte retrieval, intracytoplasmic sperm injection (ICSI) was performed. After denudation, varying degrees of debris were found in the PVS, but all the oocytes were subjected to ICSI. Among the eleven retrieved oocytes, eight were fertilized. The morphology of the embryos was scored on Days 2 and 3. Five embryos were frozen on Day 3, and two best-quality embryos were subsequently transferred via frozen embryo transfer.Conclusion: Severe debris in the PVS seems to affect embryo quality but not fertilization. Mild debris in the PVS may have little effect on the outcome of ART treatment. In our patient, after two embryos that were derived from oocytes with relatively few debris in the PVS were transferred, a successful live birth occurred.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-6"},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical pregnancy in fresh and cryopreserved cycles, using day 3 (cleavage stage) and day 4 embryos (morula stage). 使用第 3 天（卵裂期）和第 4 天（蜕膜期）的胚胎，在新鲜和冷冻周期中进行化学妊娠。

IF 1.5 4区生物学

Zygote Pub Date : 2025-03-21 DOI: 10.1017/S0967199425000048

Zahra Borzouie, Akram Hosseini, Peyman Salehi, Hatav Ghasemi Tehrani, Elham Naghshineh, Aliakbar Taherian, Mahboubeh Vatanparast

{"title":"Chemical pregnancy in fresh and cryopreserved cycles, using day 3 (cleavage stage) and day 4 embryos (morula stage).","authors":"Zahra Borzouie, Akram Hosseini, Peyman Salehi, Hatav Ghasemi Tehrani, Elham Naghshineh, Aliakbar Taherian, Mahboubeh Vatanparast","doi":"10.1017/S0967199425000048","DOIUrl":"https://doi.org/10.1017/S0967199425000048","url":null,"abstract":"With the advancement in the embryo culture media, which supports nutrient requirements of embryos up to 5 to 6 days, there's a chance to select more viable embryos, which are more likely to result in pregnancy, compared to earlier stages. Also, there is a controversy regarding the frozen embryo transfer compared to the fresh type. To compare the chemical pregnancy rates between fresh embryo transfer (ET), and frozen embryo transfer (FET), on day 3 (cleavage), and day 4 (morula) of development. In this retrospective study, data of 242 fresh and 758 frozen embryo transfer cycles were obtained in one infertility center in Isfahan, Iran. The study's groups were assigned based on the day of fresh or frozen embryo transfer (day 3, or day 4 embryos) and the embryo grading. Chemical pregnancy was the main outcome measurement (implantation rates). The chemical pregnancy rate was higher in the good quality frozen embryo day 3 and transfer on day 4 group (40.1%). This rate was near the results of transferring the good quality frozen embryo on day 4 (39.2 %). There was no significant difference in the chemical pregnancy rate related to the number of transferred embryos (p = 0.55). The higher PRs, when the embryos were transferred on day 4, provided further support for the morula stage embryo transfer, possibly because of better synchrony with the endometrium. It is concluded that morula/compact embryos are good candidates for embryo transfer, which simultaneously reduces the number of transferred embryos.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-8"},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity alters testicular gene expression in mice, monkeys and humans. 肥胖会改变老鼠、猴子和人类的睾丸基因表达。

IF 1.5 4区生物学

Zygote Pub Date : 2025-03-21 DOI: 10.1017/S0967199425000061

Wen Shao, Weijie Li, Xingjuan Yuan, Haifeng Zhang, Juan Zhao

引用次数: 0

Mineral oil viscosity and quantity impact osmotic pressure, not embryo development. 矿物油的粘度和数量影响渗透压，不影响胚胎发育。

IF 1.5 4区生物学

Zygote Pub Date : 2025-03-19 DOI: 10.1017/S0967199425000012

Hiroyuki Watanabe, Mikiko Tokoro, Masae Kojima, Chizuru Kudo, Kazuho Ihara, Noritaka Fukunaga, Yoshimasa Asada

{"title":"Mineral oil viscosity and quantity impact osmotic pressure, not embryo development.","authors":"Hiroyuki Watanabe, Mikiko Tokoro, Masae Kojima, Chizuru Kudo, Kazuho Ihara, Noritaka Fukunaga, Yoshimasa Asada","doi":"10.1017/S0967199425000012","DOIUrl":"https://doi.org/10.1017/S0967199425000012","url":null,"abstract":"Dry incubators prevent bacterial growth and allow time-lapse imaging. However, they cause more significant osmotic pressure changes in the culture medium than in humidified incubators. Few reports have explored the effect of osmotic pressure on human embryos cultured under different conditions. This study examined how changes in osmotic pressure affect human embryos in a dry incubator. The study incubated embryos in culture mediums covered with mineral oil of varying viscosities and quantities. The osmotic pressure of the culture medium was measured daily for six-day period (Day 0-6) in four experimental groups established by varying the viscosity and volume of mineral oil: low viscosity (Light), 3.0 ml or 4.5 ml, and high viscosity (Heavy), 3.0 ml or 4.5 ml of mineral oil. The Light 3.0 ml and Heavy 4.5 ml groups, showing the greatest difference in the osmotic pressure, were used to culture of human embryos. After six days of incubation, the osmotic pressure increased the most in Light 3.0 ml group. Heavy 4.5 ml group had the smallest change. However, no significant differences were noted in the formation rates of blastocysts, good-quality blastocysts, or cell count between the two groups. The study suggests that even when the culture medium is covered with heavy mineral oil in a dry incubator, osmotic pressure increases after six days but does not significantly affect the formation of blastocysts. These findings provide valuable insights into the effects of varying osmotic pressure on embryonic development and may help in optimizing conditions for in vitro fertilization.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-6"},"PeriodicalIF":1.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding CD-117 gene expression in mouse testicular germ cells: in vitro and in vivo studies. 了解CD-117基因在小鼠睾丸生殖细胞中的表达：体外和体内研究。

IF 1.5 4区生物学

Zygote Pub Date : 2025-03-17 DOI: 10.1017/S0967199424000455

Elaheh Amirinezhadfard, Pardis Arjmand, Hossein Azizi, Fatemeh Rahimi

{"title":"Understanding CD-117 gene expression in mouse testicular germ cells: in vitro and in vivo studies.","authors":"Elaheh Amirinezhadfard, Pardis Arjmand, Hossein Azizi, Fatemeh Rahimi","doi":"10.1017/S0967199424000455","DOIUrl":"https://doi.org/10.1017/S0967199424000455","url":null,"abstract":"Background: The proto-oncogene tyrosine kinase receptor encoded by the W locus (CD-117) has been confirmed to be critical to the processes of germ cell proliferation, migration and survival in the rodent. The purpose of the present study was to examine the expression of germ cell-specific CD-117 marker in testis and germ line stem cells (GSCs). The aim of this study was analysis of CD-117 expression as germ cell marker in the seminiferous tubule of mice.Materials and methods: In this experimental study, we employed a comprehensive array of techniques to scrutinize the expression of CD-117. Our analysis encompassed the utilization of immunocytochemistry, immunohistochemistry, Fluidigm real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR), and flow cytometry methodologies.Results: The Immuno-history-fluorescent analysis revealed the distribution of the germ cell marker CD-117 in the differentiated compartment of seminiferous tubules. High-magnification of confocal microscopy analysis showed surface expression of CD-117 in testis section. Whereas isolated GSCs colonies clearly express the germ-specific protein CD-117, TSCs (testicular stromal cells) were negative for this marker. Fluidigm real-time RT-PCR result demonstrated a significant expression (P < 0.001) of CD-117 in the neonate and adult GSCs compared to TSCs cells. Similarly, flow cytometry analysis confirmed expression of CD-117 in the GSCs colonies and testis cells.Conclusion: These results discriminate in spite of stage-specific ectopic, expression of CD-117 is a specific germ cell marker for proliferation and differentiation of GSCs into sperm, and can be beneficial for understanding of the signalling pathways related to differentiation of GSCs.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-5"},"PeriodicalIF":1.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of active immunization with recombinant GnRH6-kisspeptin fusion protein on reproductive function in male rats. 重组GnRH6-kisspeptin融合蛋白主动免疫对雄性大鼠生殖功能的影响。

IF 1.5 4区生物学

Zygote Pub Date : 2025-03-13 DOI: 10.1017/S0967199425000036

Mengxian Li, Xinyuan Liu, Xinbao Gong, Moyan Di, Shibao Guo, Zhihao Pan, Wei Zhang, Xu Yan, Xuelan Liu, Fugui Fang

{"title":"Effect of active immunization with recombinant GnRH6-kisspeptin fusion protein on reproductive function in male rats.","authors":"Mengxian Li, Xinyuan Liu, Xinbao Gong, Moyan Di, Shibao Guo, Zhihao Pan, Wei Zhang, Xu Yan, Xuelan Liu, Fugui Fang","doi":"10.1017/S0967199425000036","DOIUrl":"https://doi.org/10.1017/S0967199425000036","url":null,"abstract":"Immunological castration can be an alternative to traditional surgical castration. The active immunization against GnRH or kisspeptin has a castrating effect. To date, the fusion protein vaccine of combination with GnRH and kisspeptin have not been studied. Thus, the present study will develop a GnRH6-kisspeptin vaccine by genetic engineering method and investigate its immunocastration effect in male rats. Twenty 20-day-old male rats were randomly divided into two groups: the control group (n=10) and the immunization group (n=10). The initial immunization took place at week 0 followed by three booster doses administered intervals. The control group received an equivalent dose of white oil adjuvant. Orbital blood samples were collected at various time points following the initial immunization, at 0, 2, 4, 6, 8, 10 and 12 weeks, respectively. The entire left testis was weighed and its volume measured at week 12. Samples from the right testis were obtained for histological analysis. Serum levels of GnRH and kisspeptin antibodies, as well as testosterone levels were determined using ELISA. The results showed that the serum levels of GnRH and kisspeptin antibody titres of the immunized rats were significantly higher compared to the control group (P<0.05). Additionally, the testosterone concentration was effectively reduced following the intensified immunization. The testes of the immunized group exhibited a reduction in size and a significant decrease in the number of spermatogonia in the testicular tissue compared to the control group (P<0.05). These data indicate that the recombinant GnRH6-kisspeptin protein effectively induced immunological castration in rats.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-7"},"PeriodicalIF":1.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of FSH and testosterone could enhance activation of primordial follicles and growth of activated follicles in 1-day-old mice ovaries in vitro cultured for 12 days. FSH和睾酮联合使用可促进1日龄小鼠体外培养12 d卵巢原始卵泡的活化和活化卵泡的生长。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2024-12-23 DOI: 10.1017/S0967199424000479

Tahoura Torkzadeh, Zahra Asadi, Mohammad Jafari Atrabi, Maryam Khodadi, Farideh Eivazkhani, Samira Hajiaghalou, Vahid Akbarinejad, Rouhollah Fathi

{"title":"Combination of FSH and testosterone could enhance activation of primordial follicles and growth of activated follicles in 1-day-old mice ovaries in vitro cultured for 12 days.","authors":"Tahoura Torkzadeh, Zahra Asadi, Mohammad Jafari Atrabi, Maryam Khodadi, Farideh Eivazkhani, Samira Hajiaghalou, Vahid Akbarinejad, Rouhollah Fathi","doi":"10.1017/S0967199424000479","DOIUrl":"10.1017/S0967199424000479","url":null,"abstract":"Treatment with follicle-stimulating hormone (FSH) and testosterone (T2) and their combination have been observed to be influential on ovarian follicles of 1-day-old mice ovaries cultured for 8 days. Given that extension of the culture period could positively impact the development of follicles in cultured ovaries, the present study was conducted to evaluate the main and interaction effects of FSH by T2 on the development of ovarian follicles in 1-day-old mice ovaries cultured for 12 days. One-day-old mice ovaries were initially cultured with base medium for 4 days; thereafter, different hormonal treatments were added to the culture media, and the culture was continued for 8 additional days until day 12. Ovaries were collected for histological and molecular assessments on day 12. The greatest activation of primordial follicles and progression of activated follicles to the preantral stage was detected in ovaries treated with the combination of FSH and T2 (P < 0.05). This positive effect on the morphology of ovarian follicles was accompanied by upregulation of Pi3k, Gdf9, Bmp15, Cx37 and Fshr in the ovaries cultured with the combination of FSH and T2 (P < 0.05). Nonetheless, treatment with FSH and T2 led to a diminished proportion of intact follicles (P < 0.05), even though Bax/Bcl2 gene expression ratio, as an apoptotic index, was less in hormone-treated ovaries (P < 0.05). In conclusion, the combination of FSH and T2 could improve the activation of primordial follicles and the growth of activated follicles towards the preantral stage. This positive effect of FSH plus T2 appeared to be at least partly mediated through the upregulation of Pi3k and oocyte-derived growth factors including Gdf9 and Bmp15.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-9"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0