Zygote最新文献

{"title":"Relationship between follicle size, oocyte chromatin compaction, and acquisition of meiotic and developmental competences in Nellore (<i>Bos indicus</i>) cattle.","authors":"Gisele Zoccal Mingoti, Maria Isabela Azeredo Silva, Giovana Nunes, Priscila Chediek Dall'Acqua, Cíntia Rodrigues da Silva, Nathalia Rocha-Frigoni, Beatriz Caetano da Silva Leão","doi":"10.1017/S0967199425100269","DOIUrl":"10.1017/S0967199425100269","url":null,"abstract":"<p><p>This study aimed to investigate the relationship between oocyte meiotic and developmental competences and antral follicle diameter on random days of estrous in naturally cycling zebu females (Nellore - a tropical <i>Bos indicus</i> breed). Immature oocytes recovered from early antral follicles (diameter of 0.5 to 2 mm) exhibited a higher percentage of intact transzonal projections, but lower viability, smaller diameter and a higher proportion of chromatin decondensation when compared to those recovered from mid- (diameter > 2 to 6 mm) and late antral follicles (diameter > 6 mm), demonstrating that oocytes recovered from early antral follicles lack meiotic and developmental competence. Immature oocytes recovered from mid- or late antral follicles were homogeneous in terms of morphology and size, in addition to exhibiting a high proportion of a pattern of chromatin configuration compatible with high developmental competence, which may help explain the better oocyte quality and improved performance of zebu donors in IVP programs. At the end of the <i>in vitro</i> maturation culture, the percentage of oocytes that completed meiotic maturation was higher when they were recovered from late antral follicles compared to mid antral follicles, although the rate of embryonic development to the blastocyst stage was similar. Our results demonstrated a relationship between the acquisition of competences during the final stages of oocyte growth and morphofunctional events, including the chromatin structure remodeling, but further research is needed to better characterize the functional differences in ovarian physiology between bovine breeds that may impact the oocyte developmental potential.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"308-315"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146114362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First reproductive record of <i>Dekeyseria picta</i> (Siluriformes: Loricariidae): insights into embryonic development plasticity.","authors":"Ruben Dario Morales-Gamba, Armando Ortega-Lara","doi":"10.1017/S096719942610029X","DOIUrl":"10.1017/S096719942610029X","url":null,"abstract":"<p><p>This study describes the embryonic and larval development of the Atabapo butterfly pleco <i>Dekeyseria picta</i> under captive conditions. A total of 19 wild-caught adults (male-to-female ratio of 9:10) were maintained together in a single 1000-L tank operated with a recirculating aquaculture system (RAS), at approximately 26°C, under a controlled 12:12 h light-dark cycle. A total of 21 natural spawning events produced an average of 66.5 ± 2.43 spherical eggs with a mean diameter of 3.896 ± 0.022 mm, characterized by a yellow yolk and a strongly adhesive chorion. Embryonic development lasted between three and seven days, and hatched larvae were classified into five developmental phases. Early phases exhibited limited differentiation and weak pigmentation, whereas advanced phases showed visible eyes, increased pigmentation, and progressive yolk absorption. Heartbeat activity and tail movements were observed throughout all phases. The hatching rate was below 40%, likely influenced by differences in chorion rupture efficiency among embryos at distinct developmental stages. Two developmental strategies potentially contributing to embryonic plasticity were identified: (i) a diapause-like state in which embryos temporarily arrested development at early phases without yolk utilization while remaining responsive to stimuli, and (ii) delayed hatching, in which embryos remained within the chorion for up to seven days, allowing further larval development in a protected environment. These strategies may represent adaptive responses to rapidly fluctuating hydrological and environmental conditions characteristic of the natural habitat of the species and emphasize the role of aquaculture in advancing reproductive knowledge and conservation.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"316-322"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of laser-assisted thinning and laser-assisted drilling techniques on assisted reproductive outcomes.","authors":"İbrahim Pala, Funda Gode","doi":"10.1017/S0967199425100208","DOIUrl":"10.1017/S0967199425100208","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the effect of two different assisted hatching laser protocols thinning assisted hatching laser (TAH) vs drilling of assisted hatching laser (DAH) and non-assisted hatching control group (NAC) on clinical pregnancy and live birth rates in frozen thawed cleavage stage embryo transfer cycles.</p><p><strong>Patients and methods: </strong>This study included 310 infertile patients who underwent frozen embryo transfer (FET) cycles from 2021 to 2022 at the ART Unit of the Medical Point Hospital of Izmir University of Economics, Izmir. Patients included in the study were those between 20 and 40 years of age, who had undergone frozen-thawed embryo transfer after 'freeze-all' protocols. The exclusion criteria were azoospermia and degenerated embryos. In TAH, laser thinning was performed by making 4-5 shots at a depth of 50% of the thickness of the zona pellucida (ZP). In DAH, the laser opening was made from the outer part of the ZP to the inner part. In the last group in NAC, assisted hatching was not performed. Clinical pregnancy and ongoing pregnancy rates were compared between the TAH, DAH and NAC cycles.</p><p><strong>Results: </strong>There was no difference in terms of the age of the woman, the BMI and the sperm parameters in the three groups. There were no statistical differences between the groups in terms of the number of oocytes, embryos and the quality of the transferred embryos. Clinical pregnancy in thinning assisted hatching laser (TAH), drilling of assisted hatching laser (DAH), non-assisted hatching control group (NAC) cycles (38% vs 39% vs 45% <i>p</i> = 0.842, respectively.), ongoing pregnancy (34% vs 32% vs 39%; <i>p</i> = 0.670, respectively.) and live birth rates (34% vs 29% vs 35,4%; <i>p</i> = 0.586, respectively) were similar in three groups.</p><p><strong>Conclusion: </strong>In conclusion, no significant differences were found between the TAH, DAH and NAC groups in terms of ART outcomes.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"251-256"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145655916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles revolutionizing animal reproductive biotechnology: a review.","authors":"Roberto Mendes Júnior, Agostinho Soares de Alcântara Neto, Lucy Vanessa Sulca Ñaupas, Deborah de Melo Magalhães Padilha, Ana Paula Ribeiro Rodrigues","doi":"10.1017/S0967199425100233","DOIUrl":"10.1017/S0967199425100233","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are implicated in various functions within the complex mechanisms of intercellular communication. There are several subpopulations of EVs, including apoptotic bodies, microvesicles and exosomes. These nanovesicles are capable of transferring functional proteins and genetic information to alter the phenotype and function of recipient cells. In animal reproduction, numerous studies have demonstrated that EVs are actively involved in the regulation of different physiological events, modulating a variety of processes such as follicular development, spermatogenesis, oocyte maturation, fertilization and embryo development, with results indicating improved gamete quality, embryo development and cryotolerance. Additionally, EVs show therapeutic potential in restoring reproductive function and supporting maternal-embryonic communication in both domestic and wild species. Therefore, the present review aimed to describe the main studies conducted using EVs in the field of animal reproduction, highlighting their biological relevance, experimental applications and future prospects for clinical implementation in assisted reproductive technologies.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"237-250"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145757777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ethanol and sperm head reducing agents on the preimplantation development of bovine embryos generated by Piezo-ICSI.","authors":"Víctor Gallardo, Luis Aguila, María Elena Arias, Ricardo Felmer","doi":"10.1017/S096719942510021X","DOIUrl":"10.1017/S096719942510021X","url":null,"abstract":"<p><p>Intracytoplasmic sperm injection (ICSI) is a widely used assisted reproduction technique, but in cattle it faces major challenges due to inefficient oocyte activation after sperm microinjection. This study investigated different oocyte activation strategies and assessed the potential role of reducing agents glutathione (GSH), cysteamine (Cys) and dithiobutylamine (DTBA) to improve sperm head decondensation and embryo development following Piezo-ICSI. Haploid parthenogenetic activation using different ethanol concentrations (1%, 3%, 7% and 10%) failed to yield blastocysts, while diploid activation with ethanol or ionomycin combined with inhibitors significantly improved cleavage (43-55%) and blastocyst rates (14-27%), respectively. However, applying two ethanol pulses was detrimental, reducing both cleavage and blastocysts likely due to toxic overexposure. Sperm head decondensation compounds in Piezo-ICSI showed a high percentage of inactivated oocytes (75% GSH, 55% Cys and 40% DTBA). The highest male pronuclear formation rates were observed in the control without sperm head decondensation (21%) and with DTBA treatment (10%). Despite this, the treatment with Cys resulted in higher developmental potential to the blastocyst stage (22%) comparable to the control (24%). These data suggest that the inclusion of sperm head decondensing agents could represent a promising new strategy for enhancing the early <i>in vitro</i> development of ICSI-generated embryos. However, for this purpose, careful optimization of the concentration and incubation time of these decondensing compounds is essential.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"277-284"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145709499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell cycle timing and centromere positioning in <i>Bos taurus</i> zygotes derived from <i>in vitro</i> fertilization.","authors":"Federica Modafferi, Laura Thompson, Caitríona M Collins, Elena O'Callaghan, Patrick Lonergan, Elaine M Dunleavy","doi":"10.1017/S0967199425100221","DOIUrl":"10.1017/S0967199425100221","url":null,"abstract":"<p><p>Centromeres are chromosomal loci essential for the correct segregation of genetic material during cell division. Defects in centromere function can lead to aneuploidy and cancer. During early embryonic development in mammals, prior to the first cell division, male and female genomes are separated in pronuclei located at the centre of the zygote. Parental chromatin clusters at the interface between the two pronuclei and this clustering step is critical to avoid aneuploidy in human and bovine zygotes. Yet, despite their essential function in chromosome segregation, the position and spatial organization of centromeres during the first cell cycle in mammals is mostly unknown. Previous studies conducted in bovine embryos derived from <i>in vitro</i> fertilization (IVF) showed that cell cycle progression impacts on the success rate of blastocyst formation. Specifically, embryos that entered earliest into S-phase or the earliest cleaving embryos were more likely to develop into blastocysts. To determine the precise timing of these events we performed a detailed characterization of key phases of the first cell cycle in bovine zygotes derived from IVF. In parallel we examined the spatial positioning of centromeres. We identify 20 h post insemination (hpi) as the timepoint when male and female pronuclei are juxtaposed and are completing S-phase. At this timepoint, we show that centromeres are positioned distal to the pronuclear interface and use super resolution microscopy to demonstrate extensive centromere clustering into chromocentres. Our results identify distinct nuclear features observed at 20 hpi, which may serve as cell cycle markers in determining successful bovine IVF.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"257-264"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145678736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct effect of kisspeptin on steroidogenesis, proliferation and apoptosis in Tan sheep ovarian granulosa cells.","authors":"Wei Song, Tianshu Dai, Di Yang, Shuang Li, Ming Cao, Xingang Dan","doi":"10.1017/S0967199425100191","DOIUrl":"10.1017/S0967199425100191","url":null,"abstract":"<p><p>Kisspeptin, encoded by <i>Kiss1</i> gene, regulates reproduction via the hypothalamic-pituitary-gonadal axis. While kisspeptin treatment promotes follicular development in Tan sheep, its direct action on ovarian granulosa cells remains unclear. For this, we first detected the expression of <i>Kiss1</i> and its receptor <i>Kiss1r</i> in primary ovarian granulosa cells of Tan sheep. Second, the effect of kisspeptin on steroid hormone secretion, proliferation and apoptosis of ovarian granulosa cells was investigated. Third, the signaling pathway of kisspeptin regulating steroid hormone secretion was revealed by western bolting in ovarian granulosa cells. The results showed that the <i>Kiss1</i> and <i>Kiss1r</i> genes were present in ovarian granulosa cells of Tan-sheep, and 500 nM dose of kisspeptin significantly stimulated steroids hormone secretion (<i>P</i> < 0.05), and up-regulated <i>StAR</i>, <i>HSD17B</i>2, <i>CPY19A</i>1, <i>FSHR</i>, <i>LHR</i>, <i>ERβ</i>, <i>PGR</i> and p-ERK1/2 proteins expression (<i>P</i> < 0.05). Moreover, this treatment significantly promoted cell proliferation and increased the proportion of cells in S phase (<i>P</i> < 0.05), and significantly suppressed granulosa cell apoptosis (<i>P</i> < 0.05). Additionally, the stimulatory effects of kisspeptin on estradiol and progesterone secretion were blocked by inhibitors of the Mitogen-activated Protein Kinase (MAPK) signaling pathway (including PKA inhibitor, PLC inhibitor, PKC inhibitor, and Ca²⁺inhibitor). Western blot analysis confirmed that kisspeptin regulates steroid hormone secretion primarily through the MAPK-ERK signaling pathway. Our results demonstrate that kisspeptin can directly act on ovarian granulosa cells to promote steroidogenesis, proliferation, and inhibit apoptosis, providing a foundational basis for developing novel kisspeptin-mediated techniques to regulate reproduction in Tan sheep.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"265-276"},"PeriodicalIF":1.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145709097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The stereological investigation of conditioned medium originated from human adipose tissue-derived mesenchymal stem cells and platelet-rich plasma effects on polycystic ovary syndrome in a rat model.","authors":"Farzaneh Dehghani, Reza Arefnezhad, Nader Tanideh, Fatemeh Karimi, Fereshteh Aliakbari, Gholamreza Mohseni, Tahereh Esmaeilpour","doi":"10.1017/S0967199425100142","DOIUrl":"10.1017/S0967199425100142","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Platelet-rich plasma (PRP) and conditioned medium (CM) originating from human adipose tissue mesenchymal stem cells (ATMSCs) as two enriched sources of growth factors have a potential impact on tissue repair.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;This investigation aimed to investigate the effects of PRP and CM on ovarian structures in letrozole-induced polycystic ovarian syndrome (PCOS) in female rats through the stereological methods.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;To obtain PRP, blood samples from Wistar rats were collected in citrate tubes, centrifuged (400g, 10 min) to separate components. Plasma+Buffy coat was recentrifuged (800g, 10 min); Platelet-poor-plasma (PPP) discarded, PRP (lower layer) obtained and stored at - 20°C. To attain the mentioned mesenchymal CM, first, adipose tissue was collected from liposuction samples by collagenase digestion and cultured in DMEM/FBS. To approve the isolation of ATMSCs, the flow cytometry method, based on the expression status of CD44, CD90, CD34 and CD45 markers, was carried out. ATMSCs were then grown in serum-free DMEM, and supernatant was centrifuged and stored. Forty female Wistar rats were allocated into five groups (Control, negative control (letrozole [LTZ]), letrozole and PRP [LTZ-PRP], letrozole and CM [LTZ-CM] and letrozole and PRP + CM [LTZ-PRP + CM] groups). The Control group received normal saline (0.9% NaCl, 200 μl) orally. In the negative control, PCOS was induced by letrozole (1 mg/kg dissolved in normal saline, daily). Animals were treated by PRP, CM and PRP + CM (200 μL, IP, 1 and 14 days after PCOS induction). At the end of the experiment, body mass index (BMI) and body weight were evaluated, and then, blood samples were taken for the evaluation of serum testosterone level. The animals were dissected, and their ovaries were excised, fixed, sectioned and stained by H&E. Stereological approaches were utilized for estimation of the volume of the ovary, ovarian cortex and medulla, corpus luteum, oocytes, ovarian cysts and the number of different ovarian follicles and granulosa cells.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In the LTZ-PRP and LTZ-CM groups, ovarian weight (OW) was significantly increased compared to the LTZ group (&lt;i&gt;P&lt;/i&gt; &lt; 0.001 and &lt;i&gt;P&lt;/i&gt; &lt; 0.05, respectively). The cortex volume (CV) and ovarian volume (OV) of the LTZ-PRP group were dramatically elevated compared to the LTZ group (&lt;i&gt;P&lt;/i&gt; &lt; 0.0001). A remarkable elevation of the preantral follicles number (PFN) in the mentioned group compared to the LTZ group was also observed (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). The estimation of the antral follicle number (AFN), atretic follicle number (AtFN) and granulosa cell number (GCN) in the LTZ-PRP group showed a significant increment compared to the LTZ group (&lt;i&gt;P&lt;/i&gt; &lt; 0.0001). The PFN and AFN in the LTZ-CM group compared to the LTZ group were significantly elevated (&lt;i&gt;P&lt;/i&gt; &lt; 0.01). In this group, AtFN and GCN were also increased significantly in com","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"216-229"},"PeriodicalIF":1.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145132151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc oxide-curcumin nanoparticles supplementation during oocyte maturation improves bovine <i>in vitro</i> embryo production.","authors":"Luisa Miglio, Muller Carrara Martins, Serena Mares Malta, Renner Mateus Francisco Duarte, Marco Aurélio Schiavo Novaes, Laritza Ferreira de Lima, Tatiane Moraes Arantes, Rayssa de Souza Lopes, Kele Amaral Alves, Marcelo Emílio Beletti, Foued Salmen Espíndola, José Ricardo de Figueiredo","doi":"10.1017/S0967199425100129","DOIUrl":"10.1017/S0967199425100129","url":null,"abstract":"<p><p>This work investigated the effect of zinc oxide nanoparticles functionalized with curcumin (ZnO_(np)+CUR) supplementation during the <i>in vitro</i> maturation (IVM) of bovine oocytes on the <i>in vitro</i> embryo production and the cellular antioxidant response. A total of 1,625 cumulus-oocyte complexes (COCs) were cultured in the maturation medium in the absence (0 µM - control) or presence of different concentrations of ZnO_(np)+CUR (3 µM, 6 µM or 12 µM). After IVM, COCs were destined either to 1) <i>in vitro</i> embryo production or 2) analysis of reactive oxygen species production, superoxide dismutase (SOD) activity, catalase (CAT) activity and total antioxidant capacity (FRAP). The results demonstrated that the addition of 6 and 12 µM ZnO_(np)+CUR during <i>in vitro</i> maturation showed a higher rate of blastocyst production when compared to the control (<i>p</i> < 0.05). However, only 12 µM ZnO_(np)+CUR treatment showed higher rates of embryo production when compared to 3µM ZnO_(np)+CUR treatment. Supplementation of IVM medium with 6 µM ZnO_(np)+CUR reduced ROS production (<i>p</i> < 0.05) compared to control and 12 µM ZnO_(np)+CUR treatments. Also, the treatment containing ZnO_(np)+CUR at 12 µM had lower SOD activity after IVM than control treatment. In conclusion, the best outcome for <i>in vitro</i> embryo production was obtained when 6 and 12 µM ZnO_(np)+CUR was added during IVM of bovine oocytes. However, this improvement in <i>in vitro</i> embryo production was not associated with either the reduction of ROS production or SOD and CAT activities.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"210-215"},"PeriodicalIF":1.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of reversine and proTAME treatment on chromosome segregation during mouse oocyte maturation.","authors":"Shiina Yonekura, Chihiro Hasegawa, Shoma Ochi, Yoh Kinoshita, Jin Shibata, Mizuho Kobayashi, Hikari Ikema, Akifumi Nakata, Tomisato Miura, Hideaki Yamashiro","doi":"10.1017/S0967199425100117","DOIUrl":"10.1017/S0967199425100117","url":null,"abstract":"<p><p>Aneuploidy in oocytes is a leading cause of implantation failure, miscarriage and congenital disorders. During meiosis, proper timing of chromosome segregation is regulated by the spindle assembly checkpoint (SAC) and the anaphase-promoting complex/cyclosome (APC/C). However, how pharmacological manipulation of these regulatory pathways affects aneuploidy remains incompletely understood. In this study, we investigated whether SAC inhibition by reversine induces aneuploidy in mouse oocytes and whether partial inhibition of APC/C by proTAME can alleviate these errors. Germinal vesicle (GV) oocytes were matured in vitro in the presence of various concentrations of reversine. To optimize the timing of treatment, oocytes were exposed to reversine for 0, 3, 5 or 7 h, followed by culture with or without proTAME. A proTAME-only group (2.5 nM) was also included. Chromosome spreads were analyzed at the metaphase II (MII) stage to determine aneuploidy rates. Reversine (5 nM) yielded an MII maturation rate of 80.5% but induced a high aneuploidy rate of 77.0%. Sequential treatment with 2.5 nM proTAME significantly reduced aneuploidy to 33.3%. In contrast, proTAME alone led to 79.0% aneuploidy, suggesting its effect is contingent upon prior SAC disruption. These results indicate that reversine compromises chromosomal integrity, while appropriately timed, low-dose proTAME can partially rescue segregation errors. Our findings underscore the potential of pharmacologically regulating APC/C activity to reduce aneuploidy and enhance oocyte quality, offering new avenues for improving outcomes in assisted reproductive technologies.</p>","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"195-202"},"PeriodicalIF":1.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}