IF 1.7 4区生物学

Zygote Pub Date : 2026-08-01 Epub Date: 2026-08-12 DOI: 10.1017/S0967199426100574

Azam Govahi, Hadis Gholipour, Marziyeh Ajdary, Roya Hassani, Maryam Mirani, Mehdi Mehdizadeh, Rana Mehdizadeh, Mahnaz Ashrafi

{"title":"Protective effect of alpha-lipoic acid against perfluorooctanoic acid-induced toxicity on the fertility of adult male mice.","authors":"Azam Govahi, Hadis Gholipour, Marziyeh Ajdary, Roya Hassani, Maryam Mirani, Mehdi Mehdizadeh, Rana Mehdizadeh, Mahnaz Ashrafi","doi":"10.1017/S0967199426100574","DOIUrl":"10.1017/S0967199426100574","url":null,"abstract":"Introduction: Male fertility is declining, and synthetic chemicals are a major candidate for causing it. This study investigated the effect of oral consumption of perfluorooctanoic acid on sperm quality and in vitro fertilization and the therapeutic role of alpha-lipoic acid in improving its possible effects.Methods: Twenty adults male NMRI mice weighing 24-20 g were used. The male mice were divided into four groups. The control group received no drug. The sham group received only DMSO. The model group received PFOA (10 mg/kg), and the treatment group received PFOA (10 mg/kg) and ALA (dissolved in distilled water, 100 mg /kg). After the treatment period, blood, sperm and testicular tissue samples were collected from male mice. Serum testosterone level, testicular weight and histology, sperm analysis and sperm DFI were assayed. Then, using the IVF technique, fertilization rates at the 2PN, two-cell and cleavage stages were recorded.Results: Exposure of male mice to PFOA significantly reduced sperm parameters (concentration, motility, morphology, viability), testicular weight, testosterone levels, IVF rates at the 2PN, two-cell and cleavage stages and increased sperm DFI. Simultaneous administration of ALA significantly reduced these adverse effects. P < 0.05 was considered significant.Conclusions: Our findings showed that ALA can improve the harmful effects of PFOA on sperm and fertilization rate. Therefore, the use of ALA to improve IVF outcomes deserves further attention. Given the persistent presence of PFOA in the environment and its associative (not causal) link with reduced sperm quality, further research and larger studies in this area seem necessary.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"212-219"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148713826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ubiquitin-mediated degradation of RPN13 promotes porcine sperm capacitation and sperm-zona pellucida binding. 泛素介导的RPN13降解促进猪精子获能和精子与透明带结合。

IF 1.7 4区生物学

Zygote Pub Date : 2026-08-01 Epub Date: 2026-08-05 DOI: 10.1017/S0967199426100537

Zhiwei Yao, Lipeng Cao, Jie Wang, Kun Zhao, Xuan Chen, Yi Jin, Yanqiu Lv

{"title":"Ubiquitin-mediated degradation of RPN13 promotes porcine sperm capacitation and sperm-zona pellucida binding.","authors":"Zhiwei Yao, Lipeng Cao, Jie Wang, Kun Zhao, Xuan Chen, Yi Jin, Yanqiu Lv","doi":"10.1017/S0967199426100537","DOIUrl":"10.1017/S0967199426100537","url":null,"abstract":"Sperm capacitation involves biochemical and functional changes necessary for fertilization. The ubiquitin-proteasome system (UPS) regulates protein turnover, but the roles of proteasomal receptors during capacitation remain unclear. This study investigates the regulation and functional impact of proteasomal subunits RPN13 (ADRM1) and RPN2 (PSMD1) during in vitro capacitation of boar sperm. Semen samples (n = 6) were assigned to four groups: non-capacitating control (Non-Cap, 4 h in medium lacking bovine serum albumin (BSA)), capacitating control (Cap, 4 h with BSA) and groups treated with vehicle (CM + dimethyl sulfoxide (DMSO)) or the RPN13 inhibitor RA190. We measured RPN2/RPN13 levels, RPN13-associated ubiquitin signal and RPN13-RPN2 interactions (Western blot, Co-IP) and assessed sperm kinematics, intracellular Ca2+, protein tyrosine phosphorylation (pTyr), mitochondrial membrane potential (ΔΨm), ATP, ZP binding and early embryo cleavage after IVF. The Cap group showed successful capacitation, confirmed by increases in pTyr, sperm hyperactivation and Ca2+-associated Fluo-4 fluorescence. During capacitation, RPN2 and RPN13 abundance decreased, while RPN2-RPN13 association increased. RA190 treatment increased RPN13 levels, inhibited RPN13 turnover and decreased its interaction with RPN2 and the associated ubiquitin signal. Functionally, RA190 attenuated sperm hyperactivation, Ca2+-associated Fluo-4 fluorescence, protein tyrosine phosphorylation, mitochondrial membrane potential and ATP levels and was associated with reduced fertilization-related outcomes, including ZP binding and early embryo cleavage after IVF. These findings highlight the role of RPN13 in sperm capacitation and fertilization.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"173-182"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148674252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing a safe and effective cleaning method for PDMS devices derived from 3D-printed resin moulds in embryo production. 为胚胎生产中3d打印树脂模具衍生的PDMS设备建立安全有效的清洁方法。

IF 1.7 4区生物学

Zygote Pub Date : 2026-08-01 Epub Date: 2026-07-21 DOI: 10.1017/S0967199426100550

Patricia Kubo Fontes, Roksan Franko, Giuliana de Avila Ferronato, Marcella Milazzotto, Marcia de Almeida Monteiro Melo Ferraz

{"title":"Establishing a safe and effective cleaning method for PDMS devices derived from 3D-printed resin moulds in embryo production.","authors":"Patricia Kubo Fontes, Roksan Franko, Giuliana de Avila Ferronato, Marcella Milazzotto, Marcia de Almeida Monteiro Melo Ferraz","doi":"10.1017/S0967199426100550","DOIUrl":"10.1017/S0967199426100550","url":null,"abstract":"Dynamic systems for in vitro embryo production (IVEP) have long been sought to allow a higher resemblance to the physiological conditions of the oviduct and uterus. Although significant progress has been demonstrated in the microfluidic field, some challenges are still present regarding the microfluidic devices' sterilization protocol. This complication is related to the leached components from the poly(dimethylsiloxane) (PDMS). The PDMS is one of the safest materials used in soft lithography for embryo production. However, the use of moulds required for soft lithography can compromise embryo culture. This limitation arises from the restricted range of biocompatible resins available for 3D-printing of moulds. While the replacement of the biomaterial for mould production is not a straightforward option, we hypothesized that PDMS devices fabricated from such moulds could be rendered biocompatible through an optimized cleaning protocol. In this current study, we evaluated the effectiveness of sequential washes, sonication, and extended incubation in washing solutions. Our results demonstrate that a prior incubation of the PDMS devices in PBS supplemented with antibiotics and antifungal (at 4°C for 48 hours), in addition to the incubation in IVC medium (at 38.5°C for 48 hours), enables bovine embryo development within PDMS devices fabricated with moulds from a 3D printer. This optimized protocol supported IVEP at rates equivalent to standard conditions, with no increase in apoptosis or oxidative stress, validating its suitability for artificial reproductive techniques applications.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"167-172"},"PeriodicalIF":1.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148537277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concerns on use of PVP in assisted reproduction: a narrative review. 关于PVP在辅助生殖中的使用：叙述回顾。

IF 1.4 4区生物学

Zygote Pub Date : 2026-06-08 DOI: 10.1017/S0967199425100166

Arefeh Akbari Javar, Fatemeh Anbari, Keivan Lorian, Mohammad Ali Khalili

{"title":"Concerns on use of PVP in assisted reproduction: a narrative review.","authors":"Arefeh Akbari Javar, Fatemeh Anbari, Keivan Lorian, Mohammad Ali Khalili","doi":"10.1017/S0967199425100166","DOIUrl":"https://doi.org/10.1017/S0967199425100166","url":null,"abstract":"The role of Polyvinylpyrrolidone (PVP) on human gametogenesis and embryonic development has been investigated in clinical setting. PVP is routinely used in sperm handling during intracytoplasmic sperm injection (ICSI) procedure. However, the controversies about its harmful effects on embryonic cells has been debated in recent years. Some reported that PVP decreases the membrane integrity and chromatin stability and strongly affects the fine structure of spermatozoa, such as axonemal tubules, fibrous sheath, and accessory fibres. However, others claimed that PVP delayed the onset of calcium oscillations in the oocyte in ICSI setting. Therefore, PVP affects the decondensation of male pronuclear formation after sperm injection. Also, it has been reported that after injection of conventional 10%PVP to embryos, PVP solution remained in the embryos and suppressed development of these embryos. Recent studies indicated that with a decrease in PVP concentration, embryo development increased and morphokinetics improved in ICSI cycles. Also, low concentration of PVP decreased the rate of apoptosis when compared to 10%PVP. In this review, the irreversible effects of PVP on sperm and embryonic chromosomal aberrant were reported. In conclusion, it is recommended to use the low concentration of PVP in conventional ICSI programme to limit its damage on fertilization processes as well as embryo development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-5"},"PeriodicalIF":1.4,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148200092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interruption of the treatment with naproxen restored the C57BL/6 ovulation rate. 中断萘普生治疗后，C57BL/6排卵率恢复。

IF 1.7 4区生物学

Zygote Pub Date : 2026-06-01 Epub Date: 2026-07-15 DOI: 10.1017/S0967199426100525

João Pedro Lobão Gomes Castro, Vanessa Neves Guimarães, Patrick de Abreu Barbosa, Amanda Pereira Paz, Camila Stefane Ferreira, Luiza Aparecida Ansaloni Chagas Pereira, Stella Pollyanne Oliveira, Karine Sthefany Serpa Amaral Dias, Paulo Henrique Almeida Campos-Junior

{"title":"The interruption of the treatment with naproxen restored the C57BL/6 ovulation rate.","authors":"João Pedro Lobão Gomes Castro, Vanessa Neves Guimarães, Patrick de Abreu Barbosa, Amanda Pereira Paz, Camila Stefane Ferreira, Luiza Aparecida Ansaloni Chagas Pereira, Stella Pollyanne Oliveira, Karine Sthefany Serpa Amaral Dias, Paulo Henrique Almeida Campos-Junior","doi":"10.1017/S0967199426100525","DOIUrl":"10.1017/S0967199426100525","url":null,"abstract":"Naproxen is a widely used nonsteroidal anti-inflammatory drug, and previous studies have shown that its administration in mice influenced antral follicle growth and ovulation. Therefore, the present study aimed to investigate the reversibility of these effects following treatment interruption and to evaluate their impact across different mouse backgrounds. For Experiment I, C57BL/6 females (n = 20, 6 weeks of age) were treated with 10 mg/kg (low, n = 7) or 50 mg/kg (high, n = 7) of naproxen, while animals of the vehicle group received PBS + DMSO 5% (n = 6), followed by 21 days of rest. After euthanasia, the ovaries were histologically analysed. For Experiment II (C57BL/6) and Experiment III (Swiss and BALB/c), animals were treated for eight days with the same protocol, receiving 20 IU of eCG and 20 IU of hCG for ovulation induction. Recovered oocytes were collected and quantified. Results demonstrated that the treatment affected the percentage of primary follicles, which increased in the high-dose group compared with the vehicle group. Additionally, the rate of antral follicles was higher in the high group than in the vehicle and low-dose groups. The number of ovulated oocytes was not altered in BALB/c and Swiss females, as well as the number of corpora haemorrhagica and corpus luteum (p > 0.05). Overall, our findings indicate that naproxen treatment may have transient effects on folliculogenesis in C57BL/6 mice, with no impact in BALB/c or Swiss mice.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"156-165"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of in vitro maturation medium supplementation with N-acetylcysteine on the developmental competence of sheep oocytes. 体外成熟培养基中添加n -乙酰半胱氨酸对绵羊卵母细胞发育能力的影响。

IF 1.7 4区生物学

Zygote Pub Date : 2026-06-01 Epub Date: 2026-07-08 DOI: 10.1017/S0967199426100501

Sueli de Oliveira Lima, Luana Kealy Pimentel de Oliveira, Isabela Maria Lopes, Ailton Batista Pereira, Damaris Raquel Pires Dos Santos, Andreza Mayara Carneiro Lima, Érika Karoline de Oliveira Aureliano, Gizele Augusta Lemos Silva, Istefani Mota, Brenna Brenna Maria Silva de Souza, Bruna Moura Silva, Sara Rodrigues Pereira Dos Santos, Alane Pains Oliveira do Monte, Thaís Thatiane Dos Santos Souza, Mabel Freitas Cordeiro, Maria Helena Tavares de Matos, Pedro Humberto Félix de Souza, Edilson Soares Lopes Júnior

{"title":"Effect of in vitro maturation medium supplementation with N-acetylcysteine on the developmental competence of sheep oocytes.","authors":"Sueli de Oliveira Lima, Luana Kealy Pimentel de Oliveira, Isabela Maria Lopes, Ailton Batista Pereira, Damaris Raquel Pires Dos Santos, Andreza Mayara Carneiro Lima, Érika Karoline de Oliveira Aureliano, Gizele Augusta Lemos Silva, Istefani Mota, Brenna Brenna Maria Silva de Souza, Bruna Moura Silva, Sara Rodrigues Pereira Dos Santos, Alane Pains Oliveira do Monte, Thaís Thatiane Dos Santos Souza, Mabel Freitas Cordeiro, Maria Helena Tavares de Matos, Pedro Humberto Félix de Souza, Edilson Soares Lopes Júnior","doi":"10.1017/S0967199426100501","DOIUrl":"10.1017/S0967199426100501","url":null,"abstract":"The present study aimed to evaluate the effect of N-acetylcysteine (NAC) on in vitro maturation (IVM) and in vitro embryo production in sheep. Cumulus-oocyte complexes (COCs) were collected and matured in vitro in a control medium or supplemented with different concentrations of NAC (1, 1.5 and 2.0 mM). After maturation, a subset of oocytes was used for morphological assessment and chromatin configuration analysis, as well as for evaluating intracellular levels of reactive oxygen species (ROS), glutathione (GSH), mitochondrial activity and DNA fragmentation using the TUNEL assay. Another subgroup of oocytes was subjected to in vitro fertilization (IVF), and the presumed zygotes were cultured in in vitro culture (IVC) medium to assess cleavage rates. Data were statistically analysed, considering significance at P < 0.05. The concentration of 1 mM NAC resulted in lower ROS levels compared to the control and 1.5 mM groups, in addition to reducing DNA fragmentation. No significant differences (P > 0.05) were observed in GSH levels or mitochondrial activity between treated and control groups. Furthermore, oocytes treated with 1 mM NAC showed higher cleavage rates compared to the other experimental groups. In conclusion, supplementation with 1 mM NAC improves oocyte quality and early embryonic development, as demonstrated by reduced oxidative stress and DNA fragmentation, resulting in increased cleavage rates.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"132-138"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148406164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ascorbic acid and biotin modulate heat shock protein mRNA expression after thawing in rat sperm. 抗坏血酸和生物素对大鼠精子解冻后热休克蛋白mRNA表达的调节作用。

IF 1.7 4区生物学

Zygote Pub Date : 2026-06-01 Epub Date: 2026-07-07 DOI: 10.1017/S0967199426100495

Firdevs Yilmaz Dayanc, Oguz Kaan Yalcin, Aysel Eraslan Sakar

{"title":"Ascorbic acid and biotin modulate heat shock protein mRNA expression after thawing in rat sperm.","authors":"Firdevs Yilmaz Dayanc, Oguz Kaan Yalcin, Aysel Eraslan Sakar","doi":"10.1017/S0967199426100495","DOIUrl":"10.1017/S0967199426100495","url":null,"abstract":"The successful freezing of rat spermatozoa and the development of innovative cryoprotectant formulations are of great importance in the field of reproductive biotechnology. In the present study, 4 mg/mL Ascorbic acid and 20 nM Biotin were added to cryopreservation medium containing 8% lactose-monohydrate, 23% egg yolk, and 10% tris-aminomethane to investigate the freezability of rat sperm. After cryopreservation, motility, viable spermatozoon ratio, plasma membrane integrity, abnormal acrosome ratio, and mRNA levels of certain heat shock proteins that play a critical role in the sperm cryopreservation process were evaluated. After thawing, the highest motility rate was found in the ascorbic acid group (17.50 ± 3.27), which was significantly higher compared with the control group (10.13 ± 1.16) (p < 0.05). Likewise, the highest viability rate was found in the ascorbic acid group (41.75 ± 3.69), while it was found to be significantly higher compared with the control group (19.63 ± 1.44) (p < 0.05). When the cells were evaluated in terms of plasma membrane integrity and abnormal acrosome rate, no statistical difference was found between the groups (p > 0.05). The expression levels of heat shock protein-related genes were determined using the RT-qPCR technique. Addition of biotin and ascorbic acid significantly reduced Hsp40, Hsp60, and Hsp70 mRNA levels compared with the control group (p < 0.05). In conclusion, based on the evaluated parameters, it was revealed that the addition of the antioxidant Ascorbic acid and biotin to rat sperm extender provides effective protection against cryodamage during the cryopreservation process. These findings provide a valuable foundation for research into the development of next-generation mediums for sperm cryopreservation from a molecular biotechnology perspective.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"123-131"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148399562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the impact of psychopharmacological agents on spermatozoa parameters, genomic integrity and chromatin architecture. 研究精神药理学药物对精子参数、基因组完整性和染色质结构的影响。

IF 1.7 4区生物学

Zygote Pub Date : 2026-06-01 Epub Date: 2026-07-13 DOI: 10.1017/S0967199426100483

Uğurcan Altiok, Seda Karabulut, Oya Korkmaz, Lima Oria

{"title":"Investigating the impact of psychopharmacological agents on spermatozoa parameters, genomic integrity and chromatin architecture.","authors":"Uğurcan Altiok, Seda Karabulut, Oya Korkmaz, Lima Oria","doi":"10.1017/S0967199426100483","DOIUrl":"10.1017/S0967199426100483","url":null,"abstract":"This study investigates the effects of psychopharmacological drug on male reproductive health, with a specific focus on sperm parameters, DNA integrity, and chromatin structure. The aim is to understand the potential risks these medications pose to fertility. A comparative analysis was conducted involving 14 individuals undergoing psychopharmacological treatment and 15 non-users. Key seminal parameters, including volume, concentration, count, morphology, and motility, were evaluated to assess the impact on overall sperm quality. Additionally, genetic markers such as DNA fragmentation and chromatin structural integrity were measured to determine the extent of damage to genetic material. The study also analysed Protein Kinase A (PKA) expression, a critical enzyme in the cAMP-mediated signalling pathway, to explore its role in regulating sperm function. Significant statistical differences were observed in semen parameters, DNA fragmentation, chromatin integrity, and PKA protein expression between the two groups. Individuals using psychopharmacological drugs exhibited marked impairments in sperm quality and genetic markers compared to non-users. These findings suggest a potential link between psychopharmacological drug use and adverse effects on male reproductive health. The study underscores the importance of understanding the reproductive risks associated with these medications. It highlights the need for increased awareness among healthcare professionals and patients about the potential consequences of psychopharmacological treatment on fertility. Further research is essential to explore the underlying mechanisms and develop strategies to mitigate these adverse effects, ensuring better reproductive outcomes for individuals undergoing such treatments.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"148-155"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148431116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of key factors influencing clinical pregnancy outcomes in high-quality single blastocyst frozen-thawed transfer cycles. 影响高质量单囊胚冻融移植周期临床妊娠结局的关键因素分析。

IF 1.7 4区生物学

Zygote Pub Date : 2026-06-01 Epub Date: 2026-07-02 DOI: 10.1017/S0967199426100471

Xiang Li, Junlong Jia, Xiaoshan Zhang, Weiwu Liu, Yudi Luo, Xuerong Zhen, Bowen Luo, Zengyu Yang, Lingling Zhu, YuJuan Liao, Lihua Zhang

{"title":"Analysis of key factors influencing clinical pregnancy outcomes in high-quality single blastocyst frozen-thawed transfer cycles.","authors":"Xiang Li, Junlong Jia, Xiaoshan Zhang, Weiwu Liu, Yudi Luo, Xuerong Zhen, Bowen Luo, Zengyu Yang, Lingling Zhu, YuJuan Liao, Lihua Zhang","doi":"10.1017/S0967199426100471","DOIUrl":"10.1017/S0967199426100471","url":null,"abstract":"Currently, the research on the key factors which affect clinical and non-clinical pregnancy in high-quality single blastocyst transfer cycles remains relatively limited. This is particularly true for FET cycles, where the relationship between the transfer of high-quality single blastocysts and pregnancy outcomes has not been fully explored. This study aimed to identify key factors influencing clinical pregnancy outcomes in high-quality single blastocyst frozen-thawed transfer cycles to optimize assisted reproductive technology (ART). Patients under 38 years old who underwent high-quality single blastocyst frozen-thawed embryo transfer were included. Based on clinical pregnancy outcomes, they were divided into clinical pregnancy (Group A) and non-clinical pregnancy (Group B) groups. Key influencing factors were analyzed to guide the selection of blastocysts with the highest pregnancy potential.The result showed that Group B showed significantly higher age and infertility duration, but lower AMH levels, antral follicle count, and endometrial thickness on the day of transfer compared to Group A (P < 0.01). Infertility type also differed significantly (P < 0.01). Blastocyst grading differed between groups (P < 0.01), while E2, LH, P levels, embryo age, and D3 cleavage-stage cell count showed no significant differences (P > 0.05). Multivariate analysis revealed that infertility type, age, infertility duration, and endometrial thickness significantly impacted clinical pregnancy outcomes (P< 0.05), while AMH, antral follicle count, and blastocyst grading had no significant effect. All in all, clinical pregnancy outcomes are significantly influenced by age, infertility type, infertility duration, and endometrial thickness. Early treatment, optimized endometrial conditions, and selecting high-quality blastocysts are recommended to improve pregnancy rates.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"117-122"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148369830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the stereological and oxidative stress effects of hydroalcoholic extracts of Cynara scolymus L. and pioglitazone on diabetic rat ovary. 研究了猕猴桃水醇提取物和吡格列酮对糖尿病大鼠卵巢的体视学和氧化应激作用。

IF 1.7 4区生物学

Zygote Pub Date : 2026-06-01 Epub Date: 2026-06-18 DOI: 10.1017/S0967199426100379

Samaneh Karimi, Farhad Koohpeyma, Alireza Jahangiri

{"title":"Investigating the stereological and oxidative stress effects of hydroalcoholic extracts of Cynara scolymus L. and pioglitazone on diabetic rat ovary.","authors":"Samaneh Karimi, Farhad Koohpeyma, Alireza Jahangiri","doi":"10.1017/S0967199426100379","DOIUrl":"10.1017/S0967199426100379","url":null,"abstract":"Women with diabetes frequently experience ovarian dysfunction, which is a significant indication that diabetes adversely affects their reproductive health. This study investigates the efficacy of combined treatment with Pioglitazone and Cynara scolymus extract in improving ovarian function in streptozotocin-induced diabetic rats. A total of 35 female rats were divided into five groups: (1) Control (saline), (2) DM (diabetic control), (3) DM + Piog (30 mg/kg Pioglitazone), (4) DM + Cynara (400 mg/kg C. scolymus extract), and (5) DM + Piog + Cynara (combined treatment), and were administered orally for 30 consecutive days. Following treatment, histopathological and stereological analyses were performed to evaluate follicular development, corpus luteum volume, and oxidative stress markers. The results indicated that the combined treatment (DM + Piog + Cynara) led to significant improvements, including increased numbers of preantral, antral, and Graafian follicles, alongside an enhanced corpus luteum volume. Additionally, there was a notable reduction in atretic follicles and fasting blood glucose levels compared to the diabetic group. Diabetes resulted in elevated malondialdehyde (MDA) levels and decreased antioxidant enzyme activities. However, the combination therapy effectively modulated apoptotic pathways, enhancing Bcl-2 expression while reducing Caspase-9 activity. This study demonstrates that the combined therapy of C. scolymus extract and Pioglitazone mitigates diabetic-induced ovarian dysfunction by reducing oxidative stress, normalizing hormonal levels, and promoting healthy folliculogenesis. These findings suggest that this approach may serve as a promising complementary treatment for diabetes-related reproductive issues, potentially improving reproductive health.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"105-116"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148273188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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