IF 1.5 4区生物学

Zygote Pub Date : 2025-07-08 DOI: 10.1017/S0967199425100063

Zahra Bashiri, Mansoureh Movahedin, Vahid Pirhajati, Hamidreza Asgari, Morteza Koruji

引用次数: 0

Resveratrol conjugated with silica nanoparticles used for in vitro bovine oocytes maturation. 白藜芦醇结合二氧化硅纳米颗粒用于牛卵母细胞体外成熟。

IF 1.5 4区生物学

Zygote Pub Date : 2025-07-07 DOI: 10.1017/S096719942510004X

Soraia Rage Rezende, Mayara Mafra Soares, Graciele Freitas Cardoso, Deize de Cássia Antonino Rissato, Giovanna Faria de Moraes, Gustavo Pereira Cadima, Kele Amaral Alves, Benner Geraldo Alves, Tatiane Moraes Arantes, Rodrigo Otavio Decaria de Salles Rossi, Ricarda Maria Dos Santos

{"title":"Resveratrol conjugated with silica nanoparticles used for in vitro bovine oocytes maturation.","authors":"Soraia Rage Rezende, Mayara Mafra Soares, Graciele Freitas Cardoso, Deize de Cássia Antonino Rissato, Giovanna Faria de Moraes, Gustavo Pereira Cadima, Kele Amaral Alves, Benner Geraldo Alves, Tatiane Moraes Arantes, Rodrigo Otavio Decaria de Salles Rossi, Ricarda Maria Dos Santos","doi":"10.1017/S096719942510004X","DOIUrl":"https://doi.org/10.1017/S096719942510004X","url":null,"abstract":"The objective was to evaluate the use of resveratrol conjugated with silica nanoparticles during the in vitro maturation of bovine oocytes. The oocytes were divided into the following treatment groups during the maturation process: control (n = 159), resveratrol 0.5 μM (n = 158), resveratrol 1 μM (n = 155), nanoparticles conjugated with 0.5 μM resveratrol (n = 159), and nanoparticles conjugated with 1 μM resveratrol (n = 158). Several parameters were assessed, including cumulus oophorus size, reactive oxygen species (ROS) production, oocyte nuclear maturation, cell apoptosis, cleavage rates, and blastocyst production rates. Statistical analysis was conducted using Sigma Plot software (version 11) and SAS Studio, with statistical significance defined as P ≤ 0.05 for the main effects and interactions. The results indicated that the cumulus oophorus size was smaller in the resveratrol 1 μM treatment group, and the oocyte size was reduced in the nanoparticle 1 μM treatment group. No significant differences were detected between the treatment groups in terms of ROS production, oocyte maturation, or cell apoptosis. However, the resveratrol 1 μM treatment group exhibited decreased rates of cleavage and blastocyst formation. In contrast, the nanoparticles 0.5 μM and 1.0 μM treatments showed improved cleavage and blastocyst rates compared with the resveratrol 1.0 μM treatment group. In summary, while resveratrol alone at 1 μM concentration had a negative impact on cleavage and blastocyst rates, the use of silica nanoparticles conjugated with resveratrol (both 0.5 μM and 1 μM) enhanced these outcomes, suggesting a potential advantage in using nanoparticle-conjugated resveratrol for the in vitro maturation of bovine oocytes.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-6"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of time-lapse culture dishes with refrigerated medium, rather than pre-warmed, may result in better clinical outcomes. 用冷藏培养基而不是预热培养基制备延时培养皿可能会产生更好的临床结果。

IF 1.5 4区生物学

Zygote Pub Date : 2025-07-07 DOI: 10.1017/S0967199425100051

Yasong Geng, Fangfang Dai, Zhiwei Yang, Guozhen Li, Linlin Tao, Haoyang Dai, Shusong Wang, Bo Zheng

{"title":"Preparation of time-lapse culture dishes with refrigerated medium, rather than pre-warmed, may result in better clinical outcomes.","authors":"Yasong Geng, Fangfang Dai, Zhiwei Yang, Guozhen Li, Linlin Tao, Haoyang Dai, Shusong Wang, Bo Zheng","doi":"10.1017/S0967199425100051","DOIUrl":"https://doi.org/10.1017/S0967199425100051","url":null,"abstract":"This study aimed to compare the clinical outcomes of using refrigerated versus pre-warmed media for preparing time-lapse dishes in in vitro fertilization (IVF). Patients undergoing their first IVF/ICSI cycle were divided into two groups. The control group used pre-warmed culture media, while the experimental group used refrigerated culture media. The osmotic pressure of the culture droplets in both groups was tested. No statistical differences were found between the two groups' basic data. The proportion of air microbubbles affecting imaging significantly decreased (4.55% vs. 37.97%, P < 0.001) when using pre-warmed media. However, the blastocyst formation rate (56.62% vs. 49.70%, P = 0.046) and total high-quality embryo rate (22.26% vs. 17.06%, P = 0.047) were significantly higher in the refrigerated media group compared to the pre-warmed media group. The higher rate of high-quality embryos in the refrigerated media group might result in a higher single embryo transfer rate (45.10% vs. 18.52%, P = 0.020) and implantation rate (58.23% vs. 34.69%, P = 0.010). From day -1 to day 1, osmolality increased, with the P-3.5 group showing a significant elevation compared to the other three groups. After 5 days of incubation, the osmotic pressure of group R-4.0 was significantly lower than that of groups P-3.5, P-4.0 and P-3.5. In conclusion, refrigerated culture media dishes helped stabilize the osmotic pressure of the culture microenvironment and reduce water evaporation. The refrigerated group showed a higher rate of high-quality embryos and live births, although pre-warmed culture media effectively reduced the occurrence of air microbubbles that affect embryo imaging in the next day's dishes.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-7"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetyl-L-carnitine increases follicle survival and stromal cell density in cultured bovine ovarian tissues. 乙酰左旋肉碱增加培养牛卵巢组织的卵泡存活率和基质细胞密度。

IF 1.5 4区生物学

Zygote Pub Date : 2025-06-03 DOI: 10.1017/S0967199425000103

Allana M F Leitão, Antonia V N Azevedo, Bianca R Silva, Efigênia C Barbalho, Ernando I Teixeira de Assis, Francisco C Costa, Fabricio S Martins, José R V Silva

{"title":"Acetyl-L-carnitine increases follicle survival and stromal cell density in cultured bovine ovarian tissues.","authors":"Allana M F Leitão, Antonia V N Azevedo, Bianca R Silva, Efigênia C Barbalho, Ernando I Teixeira de Assis, Francisco C Costa, Fabricio S Martins, José R V Silva","doi":"10.1017/S0967199425000103","DOIUrl":"https://doi.org/10.1017/S0967199425000103","url":null,"abstract":"This study aimed to evaluate the effects of acetyl-L-carnitine on follicle survival and growth, stromal cell density and extracellular matrix, as well as on the expression of mRNA for nuclear factor erythroid 2-related factor (NRF2), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and peroxiredoxin 6 (PRDX6) in cultured bovine ovarian cortical tissues. Ovarian fragments (3 × 3 × 1 mm) were cultured for 6 days in α-MEM+ alone or supplemented with 10, 50 or 100 μM acetyl-L-carnitine at 38.5°C with 5% CO2 in humidified air. Before (non-cultured tissues) and after culture, the ovarian fragments were fixed in 4% paraformaldehyde for 12 h for histological analysis or stored at -80ºC for mRNA expression analysis of NRF2, SOD, CAT, PRDX6 and GPX1. The results showed that 100 μM acetyl-L-carnitine increased the percentages of morphologically normal follicles and stromal cell density in cultured ovarian tissues. On the other hand, acetyl-L-carnitine did not influence the percentage of collagen in ovarian tissue nor the expression of mRNAs for NRF2, SOD, CAT, PRDX6 and GPX1. In conclusion, 100 μM acetyl-L-carnitine increased follicle survival and stromal cell density in cultured bovine ovarian tissues but does not influence collagen fibre distribution or the expression of mRNAs for NRF2, SOD, CAT, PRDX6 and GPX1.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-7"},"PeriodicalIF":1.5,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding CD-117 gene expression in mouse testicular germ cells: in vitro and in vivo studies. 了解CD-117基因在小鼠睾丸生殖细胞中的表达：体外和体内研究。

IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-03-17 DOI: 10.1017/S0967199424000455

Elaheh Amirinezhadfard, Pardis Arjmand, Hossein Azizi, Fatemeh Rahimi

{"title":"Understanding CD-117 gene expression in mouse testicular germ cells: in vitro and in vivo studies.","authors":"Elaheh Amirinezhadfard, Pardis Arjmand, Hossein Azizi, Fatemeh Rahimi","doi":"10.1017/S0967199424000455","DOIUrl":"10.1017/S0967199424000455","url":null,"abstract":"Background: The proto-oncogene tyrosine kinase receptor encoded by the W locus (CD-117) has been confirmed to be critical to the processes of germ cell proliferation, migration and survival in the rodent. The purpose of the present study was to examine the expression of germ cell-specific CD-117 marker in testis and germ line stem cells (GSCs). The aim of this study was analysis of CD-117 expression as germ cell marker in the seminiferous tubule of mice.Materials and methods: In this experimental study, we employed a comprehensive array of techniques to scrutinize the expression of CD-117. Our analysis encompassed the utilization of immunocytochemistry, immunohistochemistry, Fluidigm real-time Reverse Transcription Polymerase Chain Reaction (RT-PCR), and flow cytometry methodologies.Results: The Immuno-history-fluorescent analysis revealed the distribution of the germ cell marker CD-117 in the differentiated compartment of seminiferous tubules. High-magnification of confocal microscopy analysis showed surface expression of CD-117 in testis section. Whereas isolated GSCs colonies clearly express the germ-specific protein CD-117, TSCs (testicular stromal cells) were negative for this marker. Fluidigm real-time RT-PCR result demonstrated a significant expression (P < 0.001) of CD-117 in the neonate and adult GSCs compared to TSCs cells. Similarly, flow cytometry analysis confirmed expression of CD-117 in the GSCs colonies and testis cells.Conclusion: These results discriminate in spite of stage-specific ectopic, expression of CD-117 is a specific germ cell marker for proliferation and differentiation of GSCs into sperm, and can be beneficial for understanding of the signalling pathways related to differentiation of GSCs.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"80-84"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case report of a successful pregnancy after intracytoplasmic sperm injection when all oocytes contained abnormal inclusions in the perivitelline space. 一例卵母细胞卵周间隙异常包涵体，经卵浆内单精子注射成功妊娠的病例报告。

IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-03-21 DOI: 10.1017/S096719942500005X

Juan Peng, Wenda Zou, Liyu Zhu, Xinlin Guo, Juan Zhang, Hui Li

{"title":"A case report of a successful pregnancy after intracytoplasmic sperm injection when all oocytes contained abnormal inclusions in the perivitelline space.","authors":"Juan Peng, Wenda Zou, Liyu Zhu, Xinlin Guo, Juan Zhang, Hui Li","doi":"10.1017/S096719942500005X","DOIUrl":"10.1017/S096719942500005X","url":null,"abstract":"Background: The relationship between oocyte morphology and developmental potential has been a hot research topic in assisted reproductive technology (ART). Whether inclusions in the perivitelline space (PVS) affect ART outcomes remains controversial.Case presentation: We present a case report of a 34-year-old G3P1A2 woman who sought ART treatment because of sequelae of pelvic disease. As her husband had severe oligospermia due to the stress on the day of oocyte retrieval, intracytoplasmic sperm injection (ICSI) was performed. After denudation, varying degrees of debris were found in the PVS, but all the oocytes were subjected to ICSI. Among the eleven retrieved oocytes, eight were fertilized. The morphology of the embryos was scored on Days 2 and 3. Five embryos were frozen on Day 3, and two best-quality embryos were subsequently transferred via frozen embryo transfer.Conclusion: Severe debris in the PVS seems to affect embryo quality but not fertilization. Mild debris in the PVS may have little effect on the outcome of ART treatment. In our patient, after two embryos that were derived from oocytes with relatively few debris in the PVS were transferred, a successful live birth occurred.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"74-79"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mineral oil viscosity and quantity impact osmotic pressure, not embryo development. 矿物油的粘度和数量影响渗透压，不影响胚胎发育。

IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-03-19 DOI: 10.1017/S0967199425000012

Hiroyuki Watanabe, Mikiko Tokoro, Masae Kojima, Chizuru Kudo, Kazuho Ihara, Noritaka Fukunaga, Yoshimasa Asada

{"title":"Mineral oil viscosity and quantity impact osmotic pressure, not embryo development.","authors":"Hiroyuki Watanabe, Mikiko Tokoro, Masae Kojima, Chizuru Kudo, Kazuho Ihara, Noritaka Fukunaga, Yoshimasa Asada","doi":"10.1017/S0967199425000012","DOIUrl":"10.1017/S0967199425000012","url":null,"abstract":"Dry incubators prevent bacterial growth and allow time-lapse imaging. However, they cause more significant osmotic pressure changes in the culture medium than in humidified incubators. Few reports have explored the effect of osmotic pressure on human embryos cultured under different conditions. This study examined how changes in osmotic pressure affect human embryos in a dry incubator. The study incubated embryos in culture mediums covered with mineral oil of varying viscosities and quantities. The osmotic pressure of the culture medium was measured daily for six-day period (Day 0-6) in four experimental groups established by varying the viscosity and volume of mineral oil: low viscosity (Light), 3.0 ml or 4.5 ml, and high viscosity (Heavy), 3.0 ml or 4.5 ml of mineral oil. The Light 3.0 ml and Heavy 4.5 ml groups, showing the greatest difference in the osmotic pressure, were used to culture of human embryos. After six days of incubation, the osmotic pressure increased the most in Light 3.0 ml group. Heavy 4.5 ml group had the smallest change. However, no significant differences were noted in the formation rates of blastocysts, good-quality blastocysts, or cell count between the two groups. The study suggests that even when the culture medium is covered with heavy mineral oil in a dry incubator, osmotic pressure increases after six days but does not significantly affect the formation of blastocysts. These findings provide valuable insights into the effects of varying osmotic pressure on embryonic development and may help in optimizing conditions for in vitro fertilization.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"99-104"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementation with specific branched-chain amino acids (BCAAs) affects mouse sperm parameters and testicular apoptotic gene expression. 补充特定支链氨基酸（BCAAs）影响小鼠精子参数和睾丸凋亡基因表达。

IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-05-13 DOI: 10.1017/S0967199425000073

Roya Kamali, Leila Rashki Ghaleno, Amin Izadi, Zahra-Sadat Ghasemi, Vahid Esmaeili, Nima Eila, Adel Pezeshki, Joël R Drevet, Abdolhossein Shahverdi, AliReza Alizadeh Moghadam Masouleh

{"title":"Supplementation with specific branched-chain amino acids (BCAAs) affects mouse sperm parameters and testicular apoptotic gene expression.","authors":"Roya Kamali, Leila Rashki Ghaleno, Amin Izadi, Zahra-Sadat Ghasemi, Vahid Esmaeili, Nima Eila, Adel Pezeshki, Joël R Drevet, Abdolhossein Shahverdi, AliReza Alizadeh Moghadam Masouleh","doi":"10.1017/S0967199425000073","DOIUrl":"10.1017/S0967199425000073","url":null,"abstract":"In Western diets, high consumption of meat and dairy products, known to be rich in branched-chain amino acids (BCAAs), including leucine (Leu), isoleucine (Ile), and valine (Val), as well as BCAAs supplementation itself, may have unforeseen consequences on sperm quality. In addition, bodybuilders are increasingly resorting to BCAA supplementation to build-up their muscle mass. This study aimed to assess the effect of dietary BCAAs, provided alone or in combination, on semen parameters, apoptotic gene expression, and blood amino acid (AA) profiles. To address this question and determine whether these different BCAAs have a distinct impact on sperm quality and testicular homeostasis, fifty NMRI mature male mice were exposed or not to BCAAs supplementations (control diet: CTR; CTR + Leu supplementation; CTR + Ile supplementation; CTR + Val supplementation; CTR + all three BCAA supplementation). Only valine supplementation resulted in a significant decrease in sperm concentration and viability. In addition, only valine supplementation was associated with a dramatic increase in sperm immotility. The Bax/Bcl2 ratio, an indicator of apoptosis, was found to be significantly higher in the testes of BCAA-supplemented animals when compared with the other groups. Caspase3 expression was also significantly higher in the testes of BCAA-supplemented and Val-supplemented animals. There were no significant differences in plasma AA profiles between groups. Thus, amongst BCAAs, valine supplementation appears to carry the greatest effect on sperm functional parameters and testicular apoptotic status.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"105-113"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sesquizygotic twinning: a unique twinning type rather than mechanism. 同卵双胞胎：一种独特的双胞胎类型，而不是机制。

IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-05-13 DOI: 10.1017/S0967199425000097

Yuge Tan

引用次数: 0

The role of sperm protein in mammal fertilization: insights into gamete adhesion, membrane fusion and oocyte activation. 精子蛋白在哺乳动物受精中的作用：对配子粘附、膜融合和卵母细胞激活的见解。

IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-05-13 DOI: 10.1017/S0967199425000085

Kaiyue Hu, Bo Dong, Yugang Wang, Xiangrui Meng

{"title":"The role of sperm protein in mammal fertilization: insights into gamete adhesion, membrane fusion and oocyte activation.","authors":"Kaiyue Hu, Bo Dong, Yugang Wang, Xiangrui Meng","doi":"10.1017/S0967199425000085","DOIUrl":"10.1017/S0967199425000085","url":null,"abstract":"Globally, numerous infertile couples have been assisted by extensive research on mammalian fertilization and the rapid development of Assisted Reproductive Technology (ART). However, 5%-15% of the couples that are selected for in vitro fertilization (IVF) experience a total fertilization failure (TFF), where no zygotes develop despite oocytes and semen parameters appear to be normal. Notably, an essential early event in fertilization is the binding of spermatozoa to the oocyte's external envelope, which followed by the spermatozoa-oocyte fusion. Meanwhile, oocyte activation is a crucial cellular process necessary to block polyspermy and start the development of the zygote. Improper membrane fusion of gametes has been demonstrated to be one of the mechanisms of TFF. Moreover, considering the large amount of research on sperm proteins in recent years, thus in this review, we characterize the role and molecular mechanisms of sperm proteins in the three key processes of gamete adhesion and fusion and oocyte activation, which would provide a comprehensive understanding of the role of sperm proteins in fertilization in mammals and a favourable reference for future studies in assisted reproduction due to FF.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"63-73"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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