IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2025-01-06 DOI: 10.1017/S0967199424000352

Shiori Ashibe, Yui Kobayashi, Shusuke Toishikawa, Yoshikazu Nagao

{"title":"Effects of maternal liver abnormality on in vitro maturation of bovine oocytes.","authors":"Shiori Ashibe, Yui Kobayashi, Shusuke Toishikawa, Yoshikazu Nagao","doi":"10.1017/S0967199424000352","DOIUrl":"10.1017/S0967199424000352","url":null,"abstract":"In cattle, maternal metabolic health has been suggested to influence oocyte and embryo quality. Here, we examined whether maternal liver abnormalities affected in vitro oocyte maturation by screening meiotic maturation, spindle morphology, actin filaments, and lysosomes. In oocytes from the abnormal liver group, the maturation rate (80.2%) was significantly lower compared to a control group with healthy livers (90.8%; P < 0.05). Mean spindle area in oocytes of the abnormal group (50.4 ± 3.4 μm2) was significantly larger than in the control (40.8 ± 1.6 μm2; P < 0.05). Likewise, mean spindle width in the abnormal group (8.8 ± 0.3 μm) was significantly larger than in the control group (7.8 ± 0.2 μm; P < 0.05). The proportion of cells with correctly aligned chromosomes in the abnormal group (48.0%) was significantly lower than in the control (78.3%; P < 0.05). The number of cortical actin filaments in mature oocytes of the abnormal group (299.3 ± 3.7) was significantly lower than in the control (314.7 ± 3.2; P < 0.05). The number of lysosomes in mature oocytes of the abnormal group (1363.6 ± 39.0) was significantly higher than in the control (1123.4 ± 26.3; P < 0.05). In conclusion, our findings indicate that the quality of in vitro matured oocytes is lower in cattle with liver abnormalities than in healthy cattle.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"32-37"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The temporal control and activity of maternal zsquildlike-A/hnrnpaba during zebrafish embryogenesis indicate a role in early pattern formation. 在斑马鱼胚胎发生过程中，母体zsquildlikea /hnrnpaba的时间控制和活性表明在早期模式形成中起作用。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2025-02-25 DOI: 10.1017/S0967199425000024

Nicole Molnar, Allie Capik, Amgad Ishak, Natella Maglakelidze, Luke J Pasick, Billie Reneker, Alyse Volino, Marcia L O'Connell

{"title":"The temporal control and activity of maternal zsquildlike-A/hnrnpaba during zebrafish embryogenesis indicate a role in early pattern formation.","authors":"Nicole Molnar, Allie Capik, Amgad Ishak, Natella Maglakelidze, Luke J Pasick, Billie Reneker, Alyse Volino, Marcia L O'Connell","doi":"10.1017/S0967199425000024","DOIUrl":"10.1017/S0967199425000024","url":null,"abstract":"During embryogenesis in Danio rerio (zebrafish), the earliest morphological patterning events are dependent on the precise temporal translation and/or localization of specific maternal mRNAs/proteins. Dorsoventral patterning in particular requires the translocation of maternal factors that are present in the Balbiani Body from the vegetal region of the unfertilized egg to the future dorsal side of the embryo (Fuentes et al., 2020), leading to the localized activation of the β-catenin pathway in the cells in that region. Since zebrafish are chordates, this dorsoventral patterning then leads to the formation of neural tissue on the dorsal side of the embryo. What is not yet clear is the identity of all maternal and zygotic factors that first establish dorsoventral patterning, and which factors lead to the establishment of neural versus non-neural tissue. Taking an evolutionary approach to this question, we investigated a gene in zebrafish, zsquidlike-A (hnrnpaba), that is homologous to a key dorsoventral patterning gene in fruit flies (Drosophila melanogaster) called squid (Kelley, 1993). While dorsoventral patterning in flies and fish looks quite different both morphologically and at the molecular level, we demonstrate that not only has a key dorsoventral patterning gene in flies been conserved in fish, maternal fish zsquidlike-A protein is synthesized precisely as dorsoventral patterning is unfolding in fish embryos, and in its absence, dorsoventral patterning is severely disrupted.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"45-55"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphokinetic embryo behaviour in low-prognosis patients according to the POSEIDON criteria: an analysis of 3326 injected oocytes. 根据POSEIDON标准的低预后患者的形态动力学胚胎行为：3326个注射卵母细胞的分析。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2025-03-13 DOI: 10.1017/S0967199424000480

Edson Borges, Daniela Paes Almeida Ferreira Braga, Patricia Guilherme, Assumpto Iaconelli, Amanda Sousa Setti

{"title":"Morphokinetic embryo behaviour in low-prognosis patients according to the POSEIDON criteria: an analysis of 3326 injected oocytes.","authors":"Edson Borges, Daniela Paes Almeida Ferreira Braga, Patricia Guilherme, Assumpto Iaconelli, Amanda Sousa Setti","doi":"10.1017/S0967199424000480","DOIUrl":"10.1017/S0967199424000480","url":null,"abstract":"The objective of this cohort study was to investigate whether embryo quality and morphokinetic behaviour differ in the four groups of low-prognosis women as stratified by the POSEIDON criteria. The study was performed in a private university-affiliated in vitro fertilization (IVF) centre, and included 3326 injected oocytes from 846 women undergoing ICSI cycles between March 2019 and April 2022. Kinetic markers from the point of insemination were recorded in the EmbryoScope incubator. Generalized mixed models followed by Bonferroni post hoc were used to compare morphokinetics among the POSEIDON groups. Embryos derived from patients in the POSEIDON groups 2, 3 and 4 showed significantly slower divisions compared to those from POSEIDON 1 group. The KIDScore rank was significantly lower for embryos deriving from POSEIDON groups 2, 3 and 4 (2: 4.4 ± 0.7 vs. 3: 4.2 ± 0.2 vs. 4: 3.0 ± 0.4) compared to those deriving from POSEIDON 1 group (4.8 ± 0.1, p < 0.001). Group POSEIDON 1 showed improved implantation (26.9% vs. 2: 22.4% vs. 3: 20.0% vs. 4: 14.0, p < 0.001) and miscarriage rates (5.6% vs. 2: 31.2% vs. 4: 50.0%, p = 0.013). Embryo quality and morphokinetic behaviour differ across the POSEIDON groups, being more favourable in POSEIDON group 1, as well as implantation and miscarriage rates. Embryo development was more favourable in POSEIDON group 1 (young age and adequate ovarian reserve), suggesting that oocyte quality is determinant of embryo developmental potential. These findings show the reasonability of classifying POR by the POSEIDON criteria and provide information for counselling of POR regarding their possible prognosis.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"56-61"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of sterile trout (Triploids) by chromosome set manipulation using thermal shock treatment in rainbow trout (Oncorhynchus mykiss) from Kashmir Himalayas. 利用热冲击处理克什米尔喜马拉雅山虹鳟鱼（Oncorhynchus mykiss），通过染色体组操作生产不育鳟鱼（三倍体）。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2024-12-26 DOI: 10.1017/S0967199424000509

Asim Iqbal Bazaz, Tasaduq H Shah, Farooz A Bhat, Irfan Ahmad, Basdeo Kushwaha, Ravindra Kumar, Adnan Abubakr, Bilal A Bhat, Rizwana Malik, Nafhat-Ul-Arab Naqshbandi

{"title":"Production of sterile trout (Triploids) by chromosome set manipulation using thermal shock treatment in rainbow trout (Oncorhynchus mykiss) from Kashmir Himalayas.","authors":"Asim Iqbal Bazaz, Tasaduq H Shah, Farooz A Bhat, Irfan Ahmad, Basdeo Kushwaha, Ravindra Kumar, Adnan Abubakr, Bilal A Bhat, Rizwana Malik, Nafhat-Ul-Arab Naqshbandi","doi":"10.1017/S0967199424000509","DOIUrl":"10.1017/S0967199424000509","url":null,"abstract":"Rainbow trout (Oncorhynchus mykiss) is a promising cultivable fish species with significant potential for expansion. As a cold-water fish belonging to the Salmonidae family, it requires an optimal temperature range of 10-15°C for optimal growth. This study explores a method for producing sterile rainbow trout with maximum survival rates by using heat shock treatment to enhance growth characteristics and improve aquaculture practices. A control group and four heat shock treatments were given at 26°C and 28°C for 10 min, applied 15 and 20 min after the mixing of eggs and milt, using a water bath. Among the treated groups, the highest fertilisation, hatching and yolk sac absorption rates were 90.3 ± 0.3%, 81.8 ± 0.8% and 83.9 ± 0.5%, respectively. The highest triploidy rate of 76.6 ± 3.3% was observed with a heat shock at 28°C, 20 min after fertilisation. In contrast, none of the fish from the control group were triploids. The control group demonstrated higher survival rates at fertilisation (93.1 ± 0.4%), hatching (84.2 ± 0.4%) and complete yolk sac absorption (86.2 ± 0.5%) compared to the heat-shocked groups. The diploid and triploid chromosome numbers in rainbow trout were determined to be 2n = 60 and 3n = 91, respectively. This study confirms that heat shock treatment can effectively induce triploidy in rainbow trout, with significant variations in triploidy rates depending on the temperature and timing of the shock. While heat shock can enhance the production of sterile fish, it is essential to balance the treatment parameters to maintain high survival rates. These findings contribute to the optimisation of triploidy induction techniques and support the advancement of aquaculture practices by improving the growth, management and survival rates of rainbow trout which could significantly benefit aquaculture efficiency and sustainability.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"10-18"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel microfluidic device for human sperm separation based on rheotaxis. 一种基于流变性的人类精子分离微流控装置。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2024-12-27 DOI: 10.1017/S0967199424000467

Alireza Heidarnejad, Mohammadreza Sadeghi, Saeid Arasteh, Mohammad Adel Ghiass

{"title":"A novel microfluidic device for human sperm separation based on rheotaxis.","authors":"Alireza Heidarnejad, Mohammadreza Sadeghi, Saeid Arasteh, Mohammad Adel Ghiass","doi":"10.1017/S0967199424000467","DOIUrl":"10.1017/S0967199424000467","url":null,"abstract":"This study explores the efficacy of a novel microfluidic device in isolating rheotactic sperm and assesses their advantages compared with other motile sperm. Two microfluidic devices were used in this study: the microfluidic device we designed to separate sperm based on rheotaxis and a simple passive microfluidic device. We compared the results with the density gradient centrifugation technique. Sperm attributes including concentration, morphology, viability and motility were assessed using related procedures. Statistical analyses were conducted using one-way analysis of variance. Results showed differences in sperm concentration, motility, morphology and vitality using different sperm separation techniques. The sperms separated using our microfluidic device demonstrated the highest motilities, normal morphology percentages and higher sperm vitality but significantly lower sperm concentrations. These findings suggest the potential of our microfluidic design in enhancing sperm quality. Our findings are in agreement with previous research, emphasizing the capability of microfluidics in enhancing sperm quality. Specifically, our designed microfluidic device exhibited exceptional efficacy in isolating highly motile sperm, a critical factor for successful fertilization.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"23-31"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of consecutive ejaculation on the sperm parameters in the oligo-astheno-teratozoospermia (OAT) men. 连续射精对少弱畸形精子症（OAT）男性精子参数的影响。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2025-01-06 DOI: 10.1017/S0967199424000443

Akram Hosseini, Zahra Borzouie, Parvin Khalili, Mahboubeh Vatanparast

{"title":"The effect of consecutive ejaculation on the sperm parameters in the oligo-astheno-teratozoospermia (OAT) men.","authors":"Akram Hosseini, Zahra Borzouie, Parvin Khalili, Mahboubeh Vatanparast","doi":"10.1017/S0967199424000443","DOIUrl":"10.1017/S0967199424000443","url":null,"abstract":"Recently, the World Health Organization recommendation for abstinence time for semen analysis has been challenged in some studies and many of them have supported the advantages of a second short abstinence ejaculation. More evidence is needed to approve this for clinical use. This study aimed to compare the average routine abstinence time (2-7 days) with the short time (1-2 h) on sperm quality based on functional parameters in a population of oligo-astheno-teratozoospermia (OAT) men. The semen samples were retrieved from 50 men with OAT two times: one standard 2-7 days (long ejaculation) and short duration trimming (1-2 hours later the first ejaculation). All semen parameters as well as sperm DNA integrity were compared between groups. Results showed that mean sperm concentration (10.40 vs. 8.76), total sperm count (28.53 vs. 12.24) and mean semen volume (2.69 vs. 1.40) were higher in the first ejaculation (2-7 days of abstinence), while progressive motility (20.52 vs. 13.32), non-progressive motility (53.46 vs. 48.86), morphology (2.46 vs. 1.46) and viability (83.90 vs. 77.96) were significantly higher in the second ejaculation (P < 0.05). The second sample also showed lower immotile (26.82 vs. 38.02) and DNA fragmentation (19.5 vs. 26.96) (P < 0.05). Taking all data into account, an additional short abstinence period (AP) may be a simple and helpful strategy to obtain better sperm quality in couples with male infertility causes, especially in OAT patients. The recommended current guidelines regarding the AP may need to be revisited in severe male factors.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"38-44"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the expression levels of two long non-coding RNAs, lnc-CYP11A1-1 and RP11573D15.8, in human aneuploid and euploid embryos. lnc-CYP11A1-1和RP11573D15.8两种长链非编码rna在人类非整倍体和整倍体胚胎中的表达水平评估

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2024-12-27 DOI: 10.1017/S0967199424000492

Benedict Marshall, Hakan Aytacoglu, Onder Coban, Pinar Tulay

{"title":"Assessment of the expression levels of two long non-coding RNAs, lnc-CYP11A1-1 and RP11573D15.8, in human aneuploid and euploid embryos.","authors":"Benedict Marshall, Hakan Aytacoglu, Onder Coban, Pinar Tulay","doi":"10.1017/S0967199424000492","DOIUrl":"10.1017/S0967199424000492","url":null,"abstract":"Introduction: Long non-coding RNAs (lncRNAs) are a subset of RNA molecules that have been shown to be involved in gene regulation. A lot of different pathways are involved during gametogenesis and any disturbance to these pathways may have a derogatory impact on producing a haploid gamete and thus a euploid embryo. Steroidogenesis pathway plays a crucial role in gametogenesis. The purpose of this work was to quantify the levels of lnc-CYP11A1-1 and RP11573D15.8 expression levels in aneuploid and euploid embryos. Materials and methods: A total of 20 surplus human embryos, of which 10 euploid and ten aneuploid embryos, were collected from an IVF centre. The expression levels of two lncRNAs, which have been hypothesized to regulate expression of CYP11A1, were evaluated in these embryos. RNA was extracted and used to synthesize cDNA for the experiments. Real-time polymerase chain reaction was performed to evaluate the expression levels of each lncRNA in aneuploid and euploid embryos, respectively. Results and discussion: This study shows that lnc-CYP11A1-1 was more expressed in aneuploid than in euploid embryos. RP11-573D15.8 is expressed more in aneuploid embryos than in euploid ones. The results for RP11-573D15.8 were statistically significant with a p-value of 0.02 (less than the standard threshold of p 0.05), whereas the results for lnc-CYP11A1-1 were not statistically significant with a p-value of 0.07 (greater than the standard threshold of p 0.05). Thus, the result of this study demonstrates that lncRNAs may have a role in gametogenesis and formation of aneuploid gametes.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"19-22"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of growth differentiation factor 9 expression with nuclear receptor and basic helix-loop-helix transcription factors in buffalo oocytes during in vitro maturation. 水牛卵母细胞体外成熟过程中生长分化因子 9 的表达与核受体和碱性螺旋-环-螺旋转录因子的关系。

IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI: 10.1017/S096719942400025X

Tripti Jain, Asit Jain, Surender Lal Goswami, Bhaskar Roy, Sachinandan De, Rakesh Kumar, Tirtha Kumar Datta

{"title":"Association of growth differentiation factor 9 expression with nuclear receptor and basic helix-loop-helix transcription factors in buffalo oocytes during in vitro maturation.","authors":"Tripti Jain, Asit Jain, Surender Lal Goswami, Bhaskar Roy, Sachinandan De, Rakesh Kumar, Tirtha Kumar Datta","doi":"10.1017/S096719942400025X","DOIUrl":"10.1017/S096719942400025X","url":null,"abstract":"Growth differentiation factor 9 (GDF9) is an oocyte-specific paracrine factor involved in bidirectional communication, which plays an important role in oocyte developmental competence. In spite of its vital role in reproduction, there is insufficient information about exact transcriptional control mechanism of GDF9. Hence, present study was undertaken with the aim to study the expression of basic helix-loop-helix (bHLH) transcription factors (TFs) such as the factor in the germline alpha (FIGLA), twist-related protein 1 (TWIST1) and upstream stimulating factor 1 and 2 (USF1 and USF2), and nuclear receptor (NR) superfamily TFs like germ cell nuclear factor (GCNF) and oestrogen receptor 2 (ESR2) under three different in vitro maturation (IVM) groups [follicle-stimulating hormone (FSH), insulin-like growth factor-1 (IGF1) and oestradiol)] along with all supplementation group as positive control, to understand their role in regulation of GDF9 expression. Buffalo cumulus-oocyte complexes were aspirated from abattoir-derived ovaries and matured in different IVM groups. Following maturation, TFs expression was studied at 8 h of maturation in all four different IVM groups and correlated with GDF9 expression. USF1 displayed positive whereas GCNF, TWIST1 and ESR2 revealed negative correlation with GDF9 expression. TWIST1 & ESR2 revealing negative correlation with GDF9 expression were found to be positively correlated amongst themselves also. GCNF & USF1 revealing highly significant correlation with GDF9 expression in an opposite manner were found to be negatively correlated. The present study concludes that the expression of GDF9 in buffalo oocytes remains under control through the involvement of NR and bHLH TFs.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"429-436"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosc10 deficiency in the initial segment is dispensable for sperm maturation and male fertility in mice. 小鼠的精子成熟和雄性生育能力离不开初始区段的 Exosc10。

IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-18 DOI: 10.1017/S0967199424000418

Meiyang Zhou, Junjie Yu, Yu Xu, Hong Li, Yan-Qin Feng, Xiao Wang, Fanyi Qiu, Nana Li, Zhengpin Wang

{"title":"Exosc10 deficiency in the initial segment is dispensable for sperm maturation and male fertility in mice.","authors":"Meiyang Zhou, Junjie Yu, Yu Xu, Hong Li, Yan-Qin Feng, Xiao Wang, Fanyi Qiu, Nana Li, Zhengpin Wang","doi":"10.1017/S0967199424000418","DOIUrl":"10.1017/S0967199424000418","url":null,"abstract":"EXOSC10 is an exosome-associated ribonuclease that degrades and processes a wide range of transcripts in the nucleus. The initial segment (IS) of the epididymis is crucial for sperm transport and maturation in mice by affecting the absorption and secretion that is required for male fertility. However, the role of EXOSC10 ribonuclease-mediated RNA metabolism within the IS in the regulation of gene expression and sperm maturation remains unknown. Herein, we established an Exosc10 conditional knockout (Exosc10 cKO) mouse model by crossing Exosc10 F/F mice with Lcn9-Cre mice which expressed recombinase in the principal cells of IS as early as post-natal day 17. Morphological and histological analyses revealed that Exosc10 cKO males had normal spermatogenesis and development of IS. Moreover, the sperm concentration, morphology, motility, and frequency of acrosome reactions in the cauda epididymides of Exosc10 cKO mice were comparable with those of control mice. Thus, Exosc10 cKO males had normal fertility. Collectively, our genetic mouse model and findings demonstrate that loss of EXOSC10 in the IS of epididymis is dispensable for sperm maturation and male fertility.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"437-445"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are the number of nucleolar precursor body and size of pronuclear correlated with embryo development and ploidy status in 1PN zygotes?: an analysis through the time-lapse monitoring and pre-implantation genetic testing. 在1PN受精卵中，核仁前体的数量和原核的大小是否与胚胎发育和倍性状态有关？通过延时监测和胚胎植入前基因检测进行分析。

IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-12-13 DOI: 10.1017/S0967199424000431

Shun Xiong, Jiang Wang, Yang Gao, Lihong Wu, Xiangwei Hao, Jia Hong Zhu, Keya Tong, Guoning Huang, Jun Xia Liu, Wei Han

{"title":"Are the number of nucleolar precursor body and size of pronuclear correlated with embryo development and ploidy status in 1PN zygotes?: an analysis through the time-lapse monitoring and pre-implantation genetic testing.","authors":"Shun Xiong, Jiang Wang, Yang Gao, Lihong Wu, Xiangwei Hao, Jia Hong Zhu, Keya Tong, Guoning Huang, Jun Xia Liu, Wei Han","doi":"10.1017/S0967199424000431","DOIUrl":"10.1017/S0967199424000431","url":null,"abstract":"This study aimed to demonstrate the utilization value of 1PN embryos. The 1PN zygotes collected from December 2021 to September 2022 were included in this study. The embryo development, the pronuclear characteristics, and the genetic constitutions were investigated. The overall blastocyst formation and good-quality blastocyst rates in 1PN zygotes were 22.94 and 16.24%, significantly lower than those of 2PN zygotes (63.25 and 50.23%, respectively, P = 0.000). The pronuclear characteristics were found to be correlated with the developmental potential. When comparing 1PN zygotes that developed into blastocysts to those that arrested, the former exhibited a significantly larger area (749.49 ± 142.77 vs. 634.00 ± 119.05, P = 0.000), a longer diameter of pronuclear (29.81 ± 3.08 vs. 27.30 ± 3.00, P = 0.000), and a greater number of nucleolar precursor body (NPB) (11.56 ± 3.84 vs. 7.19 ± 2.73, P = 0.000). Among the tested embryos, the diploidy euploidy rate was significantly higher in blastocysts in comparison with the arrested embryos (66.67 vs. 11.76%, P = 0.000), which was also significantly higher in IVF-1PN blastocysts than in ICSI-1PN blastocysts (75.44 vs. 25.00%, P = 0.001). However, the pronuclear characteristics were not found to be linked to the chromosomal ploidy once they formed blastocysts.In summary, while the developmental potential of 1PN zygotes is reduced, our study shows that, in addition to the reported pronuclear area and diameter, the number of NPB is also associated with their developmental potential. The 1PN blastocysts exhibit a high diploidy euploidy rate, are recommend to be clinically used post genetic testing, especially for patients who do not have other 2PN embryos available.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"459-465"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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