IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-03-13 DOI: 10.1017/S0967199425000036

Mengxian Li, Xinyuan Liu, Xinbao Gong, Moyan Di, Shibao Guo, Zhihao Pan, Wei Zhang, Xu Yan, Xuelan Liu, Fugui Fang

{"title":"Effect of active immunization with recombinant GnRH6-kisspeptin fusion protein on reproductive function in male rats.","authors":"Mengxian Li, Xinyuan Liu, Xinbao Gong, Moyan Di, Shibao Guo, Zhihao Pan, Wei Zhang, Xu Yan, Xuelan Liu, Fugui Fang","doi":"10.1017/S0967199425000036","DOIUrl":"10.1017/S0967199425000036","url":null,"abstract":"Immunological castration can be an alternative to traditional surgical castration. The active immunization against GnRH or kisspeptin has a castrating effect. To date, the fusion protein vaccine of combination with GnRH and kisspeptin have not been studied. Thus, the present study will develop a GnRH6-kisspeptin vaccine by genetic engineering method and investigate its immunocastration effect in male rats. Twenty 20-day-old male rats were randomly divided into two groups: the control group (n=10) and the immunization group (n=10). The initial immunization took place at week 0 followed by three booster doses administered intervals. The control group received an equivalent dose of white oil adjuvant. Orbital blood samples were collected at various time points following the initial immunization, at 0, 2, 4, 6, 8, 10 and 12 weeks, respectively. The entire left testis was weighed and its volume measured at week 12. Samples from the right testis were obtained for histological analysis. Serum levels of GnRH and kisspeptin antibodies, as well as testosterone levels were determined using ELISA. The results showed that the serum levels of GnRH and kisspeptin antibody titres of the immunized rats were significantly higher compared to the control group (P<0.05). Additionally, the testosterone concentration was effectively reduced following the intensified immunization. The testes of the immunized group exhibited a reduction in size and a significant decrease in the number of spermatogonia in the testicular tissue compared to the control group (P<0.05). These data indicate that the recombinant GnRH6-kisspeptin protein effectively induced immunological castration in rats.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"92-98"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity alters testicular gene expression in mice, monkeys and humans. 肥胖会改变老鼠、猴子和人类的睾丸基因表达。

IF 1.5 4区生物学

Zygote Pub Date : 2025-04-01 Epub Date: 2025-03-21 DOI: 10.1017/S0967199425000061

Wen Shao, Weijie Li, Xingjuan Yuan, Haifeng Zhang, Juan Zhao

引用次数: 0

Chemical pregnancy in fresh and cryopreserved cycles, using day 3 (cleavage stage) and day 4 embryos (morula stage). 使用第 3 天（卵裂期）和第 4 天（蜕膜期）的胚胎，在新鲜和冷冻周期中进行化学妊娠。

IF 1.5 4区生物学

Zygote Pub Date : 2025-03-21 DOI: 10.1017/S0967199425000048

Zahra Borzouie, Akram Hosseini, Peyman Salehi, Hatav Ghasemi Tehrani, Elham Naghshineh, Aliakbar Taherian, Mahboubeh Vatanparast

{"title":"Chemical pregnancy in fresh and cryopreserved cycles, using day 3 (cleavage stage) and day 4 embryos (morula stage).","authors":"Zahra Borzouie, Akram Hosseini, Peyman Salehi, Hatav Ghasemi Tehrani, Elham Naghshineh, Aliakbar Taherian, Mahboubeh Vatanparast","doi":"10.1017/S0967199425000048","DOIUrl":"https://doi.org/10.1017/S0967199425000048","url":null,"abstract":"With the advancement in the embryo culture media, which supports nutrient requirements of embryos up to 5 to 6 days, there's a chance to select more viable embryos, which are more likely to result in pregnancy, compared to earlier stages. Also, there is a controversy regarding the frozen embryo transfer compared to the fresh type. To compare the chemical pregnancy rates between fresh embryo transfer (ET), and frozen embryo transfer (FET), on day 3 (cleavage), and day 4 (morula) of development. In this retrospective study, data of 242 fresh and 758 frozen embryo transfer cycles were obtained in one infertility center in Isfahan, Iran. The study's groups were assigned based on the day of fresh or frozen embryo transfer (day 3, or day 4 embryos) and the embryo grading. Chemical pregnancy was the main outcome measurement (implantation rates). The chemical pregnancy rate was higher in the good quality frozen embryo day 3 and transfer on day 4 group (40.1%). This rate was near the results of transferring the good quality frozen embryo on day 4 (39.2 %). There was no significant difference in the chemical pregnancy rate related to the number of transferred embryos (p = 0.55). The higher PRs, when the embryos were transferred on day 4, provided further support for the morula stage embryo transfer, possibly because of better synchrony with the endometrium. It is concluded that morula/compact embryos are good candidates for embryo transfer, which simultaneously reduces the number of transferred embryos.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-8"},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of FSH and testosterone could enhance activation of primordial follicles and growth of activated follicles in 1-day-old mice ovaries in vitro cultured for 12 days. FSH和睾酮联合使用可促进1日龄小鼠体外培养12 d卵巢原始卵泡的活化和活化卵泡的生长。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2024-12-23 DOI: 10.1017/S0967199424000479

Tahoura Torkzadeh, Zahra Asadi, Mohammad Jafari Atrabi, Maryam Khodadi, Farideh Eivazkhani, Samira Hajiaghalou, Vahid Akbarinejad, Rouhollah Fathi

{"title":"Combination of FSH and testosterone could enhance activation of primordial follicles and growth of activated follicles in 1-day-old mice ovaries in vitro cultured for 12 days.","authors":"Tahoura Torkzadeh, Zahra Asadi, Mohammad Jafari Atrabi, Maryam Khodadi, Farideh Eivazkhani, Samira Hajiaghalou, Vahid Akbarinejad, Rouhollah Fathi","doi":"10.1017/S0967199424000479","DOIUrl":"10.1017/S0967199424000479","url":null,"abstract":"Treatment with follicle-stimulating hormone (FSH) and testosterone (T2) and their combination have been observed to be influential on ovarian follicles of 1-day-old mice ovaries cultured for 8 days. Given that extension of the culture period could positively impact the development of follicles in cultured ovaries, the present study was conducted to evaluate the main and interaction effects of FSH by T2 on the development of ovarian follicles in 1-day-old mice ovaries cultured for 12 days. One-day-old mice ovaries were initially cultured with base medium for 4 days; thereafter, different hormonal treatments were added to the culture media, and the culture was continued for 8 additional days until day 12. Ovaries were collected for histological and molecular assessments on day 12. The greatest activation of primordial follicles and progression of activated follicles to the preantral stage was detected in ovaries treated with the combination of FSH and T2 (P < 0.05). This positive effect on the morphology of ovarian follicles was accompanied by upregulation of Pi3k, Gdf9, Bmp15, Cx37 and Fshr in the ovaries cultured with the combination of FSH and T2 (P < 0.05). Nonetheless, treatment with FSH and T2 led to a diminished proportion of intact follicles (P < 0.05), even though Bax/Bcl2 gene expression ratio, as an apoptotic index, was less in hormone-treated ovaries (P < 0.05). In conclusion, the combination of FSH and T2 could improve the activation of primordial follicles and the growth of activated follicles towards the preantral stage. This positive effect of FSH plus T2 appeared to be at least partly mediated through the upregulation of Pi3k and oocyte-derived growth factors including Gdf9 and Bmp15.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-9"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of maternal liver abnormality on in vitro maturation of bovine oocytes. 母体肝脏异常对牛卵母细胞体外成熟的影响。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2025-01-06 DOI: 10.1017/S0967199424000352

Shiori Ashibe, Yui Kobayashi, Shusuke Toishikawa, Yoshikazu Nagao

{"title":"Effects of maternal liver abnormality on in vitro maturation of bovine oocytes.","authors":"Shiori Ashibe, Yui Kobayashi, Shusuke Toishikawa, Yoshikazu Nagao","doi":"10.1017/S0967199424000352","DOIUrl":"10.1017/S0967199424000352","url":null,"abstract":"In cattle, maternal metabolic health has been suggested to influence oocyte and embryo quality. Here, we examined whether maternal liver abnormalities affected in vitro oocyte maturation by screening meiotic maturation, spindle morphology, actin filaments, and lysosomes. In oocytes from the abnormal liver group, the maturation rate (80.2%) was significantly lower compared to a control group with healthy livers (90.8%; P < 0.05). Mean spindle area in oocytes of the abnormal group (50.4 ± 3.4 μm2) was significantly larger than in the control (40.8 ± 1.6 μm2; P < 0.05). Likewise, mean spindle width in the abnormal group (8.8 ± 0.3 μm) was significantly larger than in the control group (7.8 ± 0.2 μm; P < 0.05). The proportion of cells with correctly aligned chromosomes in the abnormal group (48.0%) was significantly lower than in the control (78.3%; P < 0.05). The number of cortical actin filaments in mature oocytes of the abnormal group (299.3 ± 3.7) was significantly lower than in the control (314.7 ± 3.2; P < 0.05). The number of lysosomes in mature oocytes of the abnormal group (1363.6 ± 39.0) was significantly higher than in the control (1123.4 ± 26.3; P < 0.05). In conclusion, our findings indicate that the quality of in vitro matured oocytes is lower in cattle with liver abnormalities than in healthy cattle.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"32-37"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The temporal control and activity of maternal zsquildlike-A/hnrnpaba during zebrafish embryogenesis indicate a role in early pattern formation. 在斑马鱼胚胎发生过程中，母体zsquildlikea /hnrnpaba的时间控制和活性表明在早期模式形成中起作用。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2025-02-25 DOI: 10.1017/S0967199425000024

Nicole Molnar, Allie Capik, Amgad Ishak, Natella Maglakelidze, Luke J Pasick, Billie Reneker, Alyse Volino, Marcia L O'Connell

{"title":"The temporal control and activity of maternal zsquildlike-A/hnrnpaba during zebrafish embryogenesis indicate a role in early pattern formation.","authors":"Nicole Molnar, Allie Capik, Amgad Ishak, Natella Maglakelidze, Luke J Pasick, Billie Reneker, Alyse Volino, Marcia L O'Connell","doi":"10.1017/S0967199425000024","DOIUrl":"10.1017/S0967199425000024","url":null,"abstract":"During embryogenesis in Danio rerio (zebrafish), the earliest morphological patterning events are dependent on the precise temporal translation and/or localization of specific maternal mRNAs/proteins. Dorsoventral patterning in particular requires the translocation of maternal factors that are present in the Balbiani Body from the vegetal region of the unfertilized egg to the future dorsal side of the embryo (Fuentes et al., 2020), leading to the localized activation of the β-catenin pathway in the cells in that region. Since zebrafish are chordates, this dorsoventral patterning then leads to the formation of neural tissue on the dorsal side of the embryo. What is not yet clear is the identity of all maternal and zygotic factors that first establish dorsoventral patterning, and which factors lead to the establishment of neural versus non-neural tissue. Taking an evolutionary approach to this question, we investigated a gene in zebrafish, zsquidlike-A (hnrnpaba), that is homologous to a key dorsoventral patterning gene in fruit flies (Drosophila melanogaster) called squid (Kelley, 1993). While dorsoventral patterning in flies and fish looks quite different both morphologically and at the molecular level, we demonstrate that not only has a key dorsoventral patterning gene in flies been conserved in fish, maternal fish zsquidlike-A protein is synthesized precisely as dorsoventral patterning is unfolding in fish embryos, and in its absence, dorsoventral patterning is severely disrupted.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"45-55"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphokinetic embryo behaviour in low-prognosis patients according to the POSEIDON criteria: an analysis of 3326 injected oocytes. 根据POSEIDON标准的低预后患者的形态动力学胚胎行为：3326个注射卵母细胞的分析。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2025-03-13 DOI: 10.1017/S0967199424000480

Edson Borges, Daniela Paes Almeida Ferreira Braga, Patricia Guilherme, Assumpto Iaconelli, Amanda Sousa Setti

{"title":"Morphokinetic embryo behaviour in low-prognosis patients according to the POSEIDON criteria: an analysis of 3326 injected oocytes.","authors":"Edson Borges, Daniela Paes Almeida Ferreira Braga, Patricia Guilherme, Assumpto Iaconelli, Amanda Sousa Setti","doi":"10.1017/S0967199424000480","DOIUrl":"10.1017/S0967199424000480","url":null,"abstract":"The objective of this cohort study was to investigate whether embryo quality and morphokinetic behaviour differ in the four groups of low-prognosis women as stratified by the POSEIDON criteria. The study was performed in a private university-affiliated in vitro fertilization (IVF) centre, and included 3326 injected oocytes from 846 women undergoing ICSI cycles between March 2019 and April 2022. Kinetic markers from the point of insemination were recorded in the EmbryoScope incubator. Generalized mixed models followed by Bonferroni post hoc were used to compare morphokinetics among the POSEIDON groups. Embryos derived from patients in the POSEIDON groups 2, 3 and 4 showed significantly slower divisions compared to those from POSEIDON 1 group. The KIDScore rank was significantly lower for embryos deriving from POSEIDON groups 2, 3 and 4 (2: 4.4 ± 0.7 vs. 3: 4.2 ± 0.2 vs. 4: 3.0 ± 0.4) compared to those deriving from POSEIDON 1 group (4.8 ± 0.1, p < 0.001). Group POSEIDON 1 showed improved implantation (26.9% vs. 2: 22.4% vs. 3: 20.0% vs. 4: 14.0, p < 0.001) and miscarriage rates (5.6% vs. 2: 31.2% vs. 4: 50.0%, p = 0.013). Embryo quality and morphokinetic behaviour differ across the POSEIDON groups, being more favourable in POSEIDON group 1, as well as implantation and miscarriage rates. Embryo development was more favourable in POSEIDON group 1 (young age and adequate ovarian reserve), suggesting that oocyte quality is determinant of embryo developmental potential. These findings show the reasonability of classifying POR by the POSEIDON criteria and provide information for counselling of POR regarding their possible prognosis.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"56-61"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of sterile trout (Triploids) by chromosome set manipulation using thermal shock treatment in rainbow trout (Oncorhynchus mykiss) from Kashmir Himalayas. 利用热冲击处理克什米尔喜马拉雅山虹鳟鱼（Oncorhynchus mykiss），通过染色体组操作生产不育鳟鱼（三倍体）。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2024-12-26 DOI: 10.1017/S0967199424000509

Asim Iqbal Bazaz, Tasaduq H Shah, Farooz A Bhat, Irfan Ahmad, Basdeo Kushwaha, Ravindra Kumar, Adnan Abubakr, Bilal A Bhat, Rizwana Malik, Nafhat-Ul-Arab Naqshbandi

{"title":"Production of sterile trout (Triploids) by chromosome set manipulation using thermal shock treatment in rainbow trout (Oncorhynchus mykiss) from Kashmir Himalayas.","authors":"Asim Iqbal Bazaz, Tasaduq H Shah, Farooz A Bhat, Irfan Ahmad, Basdeo Kushwaha, Ravindra Kumar, Adnan Abubakr, Bilal A Bhat, Rizwana Malik, Nafhat-Ul-Arab Naqshbandi","doi":"10.1017/S0967199424000509","DOIUrl":"10.1017/S0967199424000509","url":null,"abstract":"Rainbow trout (Oncorhynchus mykiss) is a promising cultivable fish species with significant potential for expansion. As a cold-water fish belonging to the Salmonidae family, it requires an optimal temperature range of 10-15°C for optimal growth. This study explores a method for producing sterile rainbow trout with maximum survival rates by using heat shock treatment to enhance growth characteristics and improve aquaculture practices. A control group and four heat shock treatments were given at 26°C and 28°C for 10 min, applied 15 and 20 min after the mixing of eggs and milt, using a water bath. Among the treated groups, the highest fertilisation, hatching and yolk sac absorption rates were 90.3 ± 0.3%, 81.8 ± 0.8% and 83.9 ± 0.5%, respectively. The highest triploidy rate of 76.6 ± 3.3% was observed with a heat shock at 28°C, 20 min after fertilisation. In contrast, none of the fish from the control group were triploids. The control group demonstrated higher survival rates at fertilisation (93.1 ± 0.4%), hatching (84.2 ± 0.4%) and complete yolk sac absorption (86.2 ± 0.5%) compared to the heat-shocked groups. The diploid and triploid chromosome numbers in rainbow trout were determined to be 2n = 60 and 3n = 91, respectively. This study confirms that heat shock treatment can effectively induce triploidy in rainbow trout, with significant variations in triploidy rates depending on the temperature and timing of the shock. While heat shock can enhance the production of sterile fish, it is essential to balance the treatment parameters to maintain high survival rates. These findings contribute to the optimisation of triploidy induction techniques and support the advancement of aquaculture practices by improving the growth, management and survival rates of rainbow trout which could significantly benefit aquaculture efficiency and sustainability.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"10-18"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel microfluidic device for human sperm separation based on rheotaxis. 一种基于流变性的人类精子分离微流控装置。

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2024-12-27 DOI: 10.1017/S0967199424000467

Alireza Heidarnejad, Mohammadreza Sadeghi, Saeid Arasteh, Mohammad Adel Ghiass

{"title":"A novel microfluidic device for human sperm separation based on rheotaxis.","authors":"Alireza Heidarnejad, Mohammadreza Sadeghi, Saeid Arasteh, Mohammad Adel Ghiass","doi":"10.1017/S0967199424000467","DOIUrl":"10.1017/S0967199424000467","url":null,"abstract":"This study explores the efficacy of a novel microfluidic device in isolating rheotactic sperm and assesses their advantages compared with other motile sperm. Two microfluidic devices were used in this study: the microfluidic device we designed to separate sperm based on rheotaxis and a simple passive microfluidic device. We compared the results with the density gradient centrifugation technique. Sperm attributes including concentration, morphology, viability and motility were assessed using related procedures. Statistical analyses were conducted using one-way analysis of variance. Results showed differences in sperm concentration, motility, morphology and vitality using different sperm separation techniques. The sperms separated using our microfluidic device demonstrated the highest motilities, normal morphology percentages and higher sperm vitality but significantly lower sperm concentrations. These findings suggest the potential of our microfluidic design in enhancing sperm quality. Our findings are in agreement with previous research, emphasizing the capability of microfluidics in enhancing sperm quality. Specifically, our designed microfluidic device exhibited exceptional efficacy in isolating highly motile sperm, a critical factor for successful fertilization.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"23-31"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the expression levels of two long non-coding RNAs, lnc-CYP11A1-1 and RP11573D15.8, in human aneuploid and euploid embryos. lnc-CYP11A1-1和RP11573D15.8两种长链非编码rna在人类非整倍体和整倍体胚胎中的表达水平评估

IF 1.5 4区生物学

Zygote Pub Date : 2025-02-01 Epub Date: 2024-12-27 DOI: 10.1017/S0967199424000492

Benedict Marshall, Hakan Aytacoglu, Onder Coban, Pinar Tulay

{"title":"Assessment of the expression levels of two long non-coding RNAs, lnc-CYP11A1-1 and RP11573D15.8, in human aneuploid and euploid embryos.","authors":"Benedict Marshall, Hakan Aytacoglu, Onder Coban, Pinar Tulay","doi":"10.1017/S0967199424000492","DOIUrl":"10.1017/S0967199424000492","url":null,"abstract":"Introduction: Long non-coding RNAs (lncRNAs) are a subset of RNA molecules that have been shown to be involved in gene regulation. A lot of different pathways are involved during gametogenesis and any disturbance to these pathways may have a derogatory impact on producing a haploid gamete and thus a euploid embryo. Steroidogenesis pathway plays a crucial role in gametogenesis. The purpose of this work was to quantify the levels of lnc-CYP11A1-1 and RP11573D15.8 expression levels in aneuploid and euploid embryos. Materials and methods: A total of 20 surplus human embryos, of which 10 euploid and ten aneuploid embryos, were collected from an IVF centre. The expression levels of two lncRNAs, which have been hypothesized to regulate expression of CYP11A1, were evaluated in these embryos. RNA was extracted and used to synthesize cDNA for the experiments. Real-time polymerase chain reaction was performed to evaluate the expression levels of each lncRNA in aneuploid and euploid embryos, respectively. Results and discussion: This study shows that lnc-CYP11A1-1 was more expressed in aneuploid than in euploid embryos. RP11-573D15.8 is expressed more in aneuploid embryos than in euploid ones. The results for RP11-573D15.8 were statistically significant with a p-value of 0.02 (less than the standard threshold of p 0.05), whereas the results for lnc-CYP11A1-1 were not statistically significant with a p-value of 0.07 (greater than the standard threshold of p 0.05). Thus, the result of this study demonstrates that lncRNAs may have a role in gametogenesis and formation of aneuploid gametes.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"19-22"},"PeriodicalIF":1.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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