IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-12-13 DOI: 10.1017/S0967199424000431

Shun Xiong, Jiang Wang, Yang Gao, Lihong Wu, Xiangwei Hao, Jia Hong Zhu, Keya Tong, Guoning Huang, Jun Xia Liu, Wei Han

{"title":"Are the number of nucleolar precursor body and size of pronuclear correlated with embryo development and ploidy status in 1PN zygotes?: an analysis through the time-lapse monitoring and pre-implantation genetic testing.","authors":"Shun Xiong, Jiang Wang, Yang Gao, Lihong Wu, Xiangwei Hao, Jia Hong Zhu, Keya Tong, Guoning Huang, Jun Xia Liu, Wei Han","doi":"10.1017/S0967199424000431","DOIUrl":"10.1017/S0967199424000431","url":null,"abstract":"This study aimed to demonstrate the utilization value of 1PN embryos. The 1PN zygotes collected from December 2021 to September 2022 were included in this study. The embryo development, the pronuclear characteristics, and the genetic constitutions were investigated. The overall blastocyst formation and good-quality blastocyst rates in 1PN zygotes were 22.94 and 16.24%, significantly lower than those of 2PN zygotes (63.25 and 50.23%, respectively, P = 0.000). The pronuclear characteristics were found to be correlated with the developmental potential. When comparing 1PN zygotes that developed into blastocysts to those that arrested, the former exhibited a significantly larger area (749.49 ± 142.77 vs. 634.00 ± 119.05, P = 0.000), a longer diameter of pronuclear (29.81 ± 3.08 vs. 27.30 ± 3.00, P = 0.000), and a greater number of nucleolar precursor body (NPB) (11.56 ± 3.84 vs. 7.19 ± 2.73, P = 0.000). Among the tested embryos, the diploidy euploidy rate was significantly higher in blastocysts in comparison with the arrested embryos (66.67 vs. 11.76%, P = 0.000), which was also significantly higher in IVF-1PN blastocysts than in ICSI-1PN blastocysts (75.44 vs. 25.00%, P = 0.001). However, the pronuclear characteristics were not found to be linked to the chromosomal ploidy once they formed blastocysts.In summary, while the developmental potential of 1PN zygotes is reduced, our study shows that, in addition to the reported pronuclear area and diameter, the number of NPB is also associated with their developmental potential. The 1PN blastocysts exhibit a high diploidy euploidy rate, are recommend to be clinically used post genetic testing, especially for patients who do not have other 2PN embryos available.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"459-465"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142819499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin-transferrin-selenium supplementation improves porcine embryo production in vitro. 补充胰岛素-转铁蛋白-硒可提高猪胚胎的体外生产。

IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-25 DOI: 10.1017/S0967199424000200

Juan Lin, Zhuqing Ji, Shenming Zeng

引用次数: 0

Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera: signalling pathways involved. 脱氢亮氨酸（DhL）对栗鼠精子顶体反应的影响：相关信号通路。

IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-19 DOI: 10.1017/S096719942400042X

Maria Fernanda Medina, Ana Pucci, Franco José Pucci, Marta Ines Bühler, María Cecilia Gramajo-Bühler

{"title":"Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera: signalling pathways involved.","authors":"Maria Fernanda Medina, Ana Pucci, Franco José Pucci, Marta Ines Bühler, María Cecilia Gramajo-Bühler","doi":"10.1017/S096719942400042X","DOIUrl":"10.1017/S096719942400042X","url":null,"abstract":"The secondary metabolites of several plant species, particularly sesquiterpenic lactones (SLs) have been studied by different research groups for over 30 years. This group of metabolites presents numerous biological activities such as antibacterial, antiviral, antiulcer, cell proliferation inhibitor, and oocyte activator with participation in exocytosis processes. This study aims to assess some sperm parameters in epididymal gametes of Chichilla lanigera exposed to increasing concentrations (0 to 2 mM) of DhL for various incubation times from 10 to 40 minutes. We determined the participation of different cell signalling pathways in the induced acrosome reaction. Our results showed an alteration in the progressive motility pattern and cell viability depending on DhL concentration and exposure time of gametes. When analyzing acrosomal status, higher percentages than the negative control were obtained in all tested doses. Both isolated and joint inhibition tests of PKA and phospholipases (PLC and PLA2) showed a greater participation of PI-PLC. This is the first report concerning the effects of this lactone on the medium of sperm incubation. Consequently, further studies will be necessary to determine the molecular implications of this lactone on the fertilizing potential of the sperm.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"446-451"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of substance exposure on gametes and pre-implantation embryos: a narrative review. 物质接触对配子和植入前胚胎的影响：叙述性综述。

IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI: 10.1017/S0967199424000303

Shayesteh Mehdinejadiani, Zahra Khosravizadeh, Akram Alizadeh, Nahid Azad

引用次数: 0

Oocyte competence develops: nuclear maturation synchronously with cytoplasm maturation. 卵母细胞能力的发展：核成熟与细胞质成熟同步进行。

IF 1.5 4区生物学

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-18 DOI: 10.1017/S0967199424000169

Hossein Torkashvand, Ronak Shabani, Tayebe Artimani, Iraj Amiri, Shamim Pilehvari, Leila Torkashvand, Rana Mehdizadeh, Mehdi Mehdizadeh

{"title":"Oocyte competence develops: nuclear maturation synchronously with cytoplasm maturation.","authors":"Hossein Torkashvand, Ronak Shabani, Tayebe Artimani, Iraj Amiri, Shamim Pilehvari, Leila Torkashvand, Rana Mehdizadeh, Mehdi Mehdizadeh","doi":"10.1017/S0967199424000169","DOIUrl":"10.1017/S0967199424000169","url":null,"abstract":"Human oocyte maturation is a lengthy process that takes place over the course of which oocytes gain the inherent ability to support the next developmental stages in a progressive manner. This process includes intricate and distinct events related to nuclear and cytoplasmic maturation. Nuclear maturation includes mostly chromosome segregation, whereas rearrangement of organelles, storage of mRNAs and transcription factors occur during cytoplasmic maturation.Human oocyte maturation, both in vivo and in vitro, occurs through a process that is not yet fully understood. However, it is believed that the second messenger, cyclic adenosine monophosphate (cAMP), plays a pivotal role in the upkeep of the meiotic blocking of the human oocyte. Relatively high levels of cAMP in the human oocyte are required to maintain meiosis blocked, whereas lower levels of cAMP in the oocyte enable meiosis to resume. Oocyte cAMP concentration is controlled by a balance between adenylate cyclase and phosphodiesterases, the enzymes responsible for cAMP generation and breakdown.In addition to nuclear maturation, the female gamete requires a number of complicated structural and biochemical modifications in the cytoplasmic compartment to be able to fertilize normally. According to ultrastructural studies, during the transition from the germinal vesicle stage to metaphase II (MII), several organelles reorganize their positions. The cytoskeletal microfilaments and microtubules found in the cytoplasm facilitate these movements and regulate chromosomal segregation.The aim of this review is to focus on the nuclear and cytoplasmic maturation by investigating the changes that take place in the process of oocytes being competent for development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"421-428"},"PeriodicalIF":1.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Control of sperm penetration using stereumamide A derived from Trichaptum fuscoviolaceum in the in vitro fertilization of pig oocytes. 在猪卵母细胞体外受精过程中使用从毛癣菌（Trichaptum fuscoviolaceum）中提取的立体酰胺 A 控制精子穿透。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-25 DOI: 10.1017/S0967199424000327

Young-Joo Yi, Yoon-Ju Lee, Adikari Arachchige Dilki Indrachapa Adikari, Bong-Sik Yun

{"title":"Control of sperm penetration using stereumamide A derived from Trichaptum fuscoviolaceum in the in vitro fertilization of pig oocytes.","authors":"Young-Joo Yi, Yoon-Ju Lee, Adikari Arachchige Dilki Indrachapa Adikari, Bong-Sik Yun","doi":"10.1017/S0967199424000327","DOIUrl":"10.1017/S0967199424000327","url":null,"abstract":"Fungal metabolites are known to have potent and diverse properties such as antiviral, antidiabetic, antitumour, antioxidant, free radical scavenging, and antibacterial effects which can be utilized to treat diseases. In this study, we investigated the functional activity of stereumamide A (StA) derived from a culture broth of Trichaptum fuscoviolaceum during the in vitro fertilization (IVF) of pig oocytes, to determine its effects on sperm penetration. Oocytes matured in vitro were fertilized in the absence or presence of varying concentrations of StA (0-50 μg/ml StA). When StA was directly added into the IVF medium, significantly lower fertilization rates were seen with the 20 or 50 μg/ml StA (2.0-17.5%) treatments compared with those of 10 μg/ml StA or the controls (60.9-62.3%), whereas StA had no influence on the survival of oocytes and spermatozoa throughout the IVF process. For evaluating the control of sperm entry, mature oocytes were pre-incubated in a medium containing 20 μg/ml StA for 1 h, and then IVF was subsequently performed. The incidence of polyspermy was significantly reduced when oocytes were pre-incubated with StA (15.0% vs. 50.4-57.5% in controls). In conclusion, sperm penetration was inhibited in the medium in the presence of StA during IVF, while StA did not affect sperm motility and fertility competence. Fertilization was controlled when mature oocytes were incubated with StA prior to IVF, suggesting the possible use of the fungal metabolite in assisted reproductive technology for humans and animals.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"354-359"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the effect of serotonin-activated semen washing medium on sperm motility at the molecular level: a pilot study. 血清素激活精液洗涤介质对精子活力分子水平影响的调查：一项试验性研究。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-11-11 DOI: 10.1017/S0967199424000406

Elnaz Moshfeghi, Yasemin Yilmazer, Sinem Dogan, Turgut Aydin, Necati Findikli, Tulin Ozbek

{"title":"Investigation of the effect of serotonin-activated semen washing medium on sperm motility at the molecular level: a pilot study.","authors":"Elnaz Moshfeghi, Yasemin Yilmazer, Sinem Dogan, Turgut Aydin, Necati Findikli, Tulin Ozbek","doi":"10.1017/S0967199424000406","DOIUrl":"10.1017/S0967199424000406","url":null,"abstract":"In Assisted Reproductive Technologies (ART), efficient sperm preparation is vital for successful fertilization, with washing media enhancing the process. This pilot study examines the molecular-level impact of a new serotonin-containing sperm-washing medium (Prototype) on sperm motility and ROS metabolism, comparing it with commercially available media (Origio and Irvine). Semen samples from thirty-one individuals underwent preparation using the swim-up method post-semen analysis. Each sample was separately washed with the Prototype, Origio and Irvine mediums. ROS formation was determined through flow cytometric, and AT2R and PRDX2 protein levels, associated with sperm motility, were assessed via Western blot. Statistical evaluation compared the findings among the three outlined media. Significant differences were found among three washing media in terms of total and progressive motility. The Prototype medium showed the highest increase in both total (66%) and progressive motility (59%), while the control group exhibited the lowest increases (41% and 27.7%, respectively). Regarding ROS levels, the prototype (11.5%) and Origio (10.7%) groups demonstrated a notable decrease, contrasting with Irvine (25.8%). Molecular assessment revealed a significant elevation in AT2R protein levels in the prototype medium (59%), compared to other media. Additionally, an increase in PRDX2 protein levels was observed in the prototype medium, although this didn't reach statistical significance. Serotonin-activated washing media for sperm preparation can be a suitable choice for selecting high-quality sperm in ART. A broader molecular analysis with a larger sample size is required to explore the mechanisms and effectiveness of using a serotonin-containing sperm-washing medium in routine ART.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"396-404"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative stress. 用 GYY4137 处理人类精子可提高精子活力和抗氧化能力。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-30 DOI: 10.1017/S0967199424000340

Yan Huang, Runxin Gan, Min Zhang, Dewei Lin, Yi Cheng, Xinyu Guo

{"title":"Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative stress.","authors":"Yan Huang, Runxin Gan, Min Zhang, Dewei Lin, Yi Cheng, Xinyu Guo","doi":"10.1017/S0967199424000340","DOIUrl":"10.1017/S0967199424000340","url":null,"abstract":"Hydrogen sulfide (H2S) has been shown to play a significant role in oxidative stress across various tissues and cells; however, its role in sperm function remains poorly understood. This study aimed to investigate the protective effect of GYY4137, a slow-releasing H2S compound, on sperm damage induced by H2O2. We assessed the effects of GYY4137 on motility, viability, lipid peroxidation and caspase-3 activity in human spermatozoa in vitro following oxidative damage mediated by H2O2. Spermatozoa from 25 healthy men were selected using a density gradient centrifugation method and cultured in the presence or absence of 10 μM H2O2, followed by incubation with varying concentrations of GYY4137 (0.625-2.5 μM). After 24 h of incubation, sperm motility, viability, lipid peroxidation, and caspase-3 activity were evaluated. The results indicated that H2O2 adversely affected sperm parameters, reducing motility and viability, while increasing oxidative stress, as evidenced by elevated lipid peroxidation and caspase-3 activity. GYY4137 provided dose-dependent protection against H2O2-induced oxidative stress (OS). We concluded that supplementation with GYY4137 may offer antioxidant protection during in vitro sperm preparation for assisted reproductive technology.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"360-365"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of L-carnitine in the control of oxidative stress and lipid β-oxidation during in vitro follicle growth, oocyte maturation, embryonic development and cryopreservation: a review. 左旋肉碱在体外卵泡生长、卵母细胞成熟、胚胎发育和冷冻保存过程中控制氧化应激和脂质β氧化的作用：综述。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-11-07 DOI: 10.1017/S096719942400039X

Allana Maria Freire Leitão, Bianca Regia Silva, Efigênia C Barbalho, Lais R M Paulino, Francisco das Chagas Costa, Fabricio Sousa Martins, Jose Roberto V Silva

引用次数: 0

Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows (Bos indicus). 通过经阴道取卵术从 FSH 刺激的塔帕卡尔供体母牛（Bos indicus）中获取卵母细胞，对其发育能力进行形态分子评估。

IF 1.5 4区生物学

Zygote Pub Date : 2024-10-01 Epub Date: 2024-10-29 DOI: 10.1017/S0967199424000376

Manoj Donadkar, Brijesh Kumar, Sanjay Kumar Singh, Pradeep Chandra, Pradeep Dangi, Mohan Gawai, Amala Jackson, Nancy Jasrotia, Shweta Sharma, Vikrant Chouhan, M K Patra, Meraj Haider Khan

{"title":"Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows (Bos indicus).","authors":"Manoj Donadkar, Brijesh Kumar, Sanjay Kumar Singh, Pradeep Chandra, Pradeep Dangi, Mohan Gawai, Amala Jackson, Nancy Jasrotia, Shweta Sharma, Vikrant Chouhan, M K Patra, Meraj Haider Khan","doi":"10.1017/S0967199424000376","DOIUrl":"10.1017/S0967199424000376","url":null,"abstract":"The study was conducted on indigenous Tharparkar cow (Bos indicus) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimulated at the dose of 130 µg divided into four sub-doses and non-stimulated. Findings on the size of follicles having ≥6 mm showed a significantly higher, however an opposite trend was observed in the case of smaller sized follicle (<6 mm) between stimulated and non-stimulated respectively. The stimulated cows had a significantly higher number as well as the percentage of oocytes of Grade A, having a diameter ≥120 µm and BCB+VE as compared to the non-stimulated cows. The relative mRNA expression profile of GDF9, BMP15, PCNA and BCL-2 genes was higher and BAX was lower in the FSH-stimulated cow. These results indicated that FSH stimulation before OPU in Bos indicus cows has a significant impact on follicle size, oocyte yield, recovery, and their quality with respect to COC's, diameter and BCB+VE oocytes. Further, a significant increase in the relative mRNA expression levels of GDF9, BMP15 and PCNA genes in the FSH-stimulated group suggests that FSH plays a key role in modulating the expression of these important candidate genes and thus influencing oocyte quality. The higher mRNA expression of BCL-2 genes and concomitantly lower expression of BAX gene in FSH Stimulated cows indicates the protective role of these genes and preventing programmed cell death and thus promoting cell survival, quality and embryo development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"376-385"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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