IF 1.7 4区生物学

Zygote Pub Date : 2026-06-01 Epub Date: 2026-07-09 DOI: 10.1017/S0967199426100513

Ayse Altun Akpınar, Murat Dursun, Burcin Karamustafaoglu Balcı, Erva Sevic Yılmaz, Kübra Nur Uzun, Ates Kadioglu, Cemil Akgul

{"title":"Unveiling the role of galectin-3 in patients with azoospermia: potential biomarker for male infertility.","authors":"Ayse Altun Akpınar, Murat Dursun, Burcin Karamustafaoglu Balcı, Erva Sevic Yılmaz, Kübra Nur Uzun, Ates Kadioglu, Cemil Akgul","doi":"10.1017/S0967199426100513","DOIUrl":"10.1017/S0967199426100513","url":null,"abstract":"Objective: To investigate the role of the protein galectin-3 (Gal-3) and its effect on fibrosis and apoptosis in testicular tissues of patients with idiopathic non-obstructive azoospermia (iNOA).Design: A prospective study.Subject: Male patients who needed testicular sperm extraction for assisted reproductive technology treatments.Exposure: Tissue samples were obtained during microdissection testicular sperm extraction (mTESE) procedures from patients diagnosed as having azoospermia according to two semen analyses. Two groups were determined as sperm non-detected (n = 8, TESE-) and detected (n = 7, TESE+). Germ cells were morphologically evaluated using haematoxylin and eosin-stained sections and Johnsen scores. Immunohistochemical methods were used to evaluate Gal-3 expression and caspase-3-related apoptotic activity.Main outcome measures: Gal-3 positivity, apoptosis and fibrosis indexes.Results: Gal-3 expression was significantly higher in the TESE- group (p = 0.024). Gal-3 showed strong positivity in Sertoli Cells and cells in the interstitial area around the seminiferous tubules in the TESE- group.Conclusion: This study highlights the potential role of Gal-3 in iNOA and its association with testicular fibrosis and impaired spermatogenesis. The absence of sperm retrieval was associated with increased Gal-3 expression, seminiferous tubule wall thickening, and enhanced apoptosis-related activity. These findings suggest that Gal-3 may contribute to inflammation- and fibrosis-related testicular damage in iNOA. However, further experimental and clinical studies are required to clarify the underlying molecular mechanisms of Gal-3 and determine its potential clinical significance in male infertility.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"139-147"},"PeriodicalIF":1.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early developmental stages of Amazonian pacu Prosomyleus rhomboidalis derived from in loco reproduction. 亚马逊河鼠拟菱形豆的早期发育阶段源自于原位繁殖。

IF 1.4 4区生物学

Zygote Pub Date : 2026-05-29 DOI: 10.1017/S0967199426100392

Leydiane da Paixão Serra, Alice Xavier-Soares, Marina Pereira da Silva, Michel Martins Bandeira, Amanda Pereira Dos Santos Silva, Allan David Silva, Sandro Tetsuo Emoto, Matheus Pereira-Santos, Jose Augusto Senhorini, Leandro Melo de Sousa, George Shigueki Yasui

{"title":"Early developmental stages of Amazonian pacu Prosomyleus rhomboidalis derived from in loco reproduction.","authors":"Leydiane da Paixão Serra, Alice Xavier-Soares, Marina Pereira da Silva, Michel Martins Bandeira, Amanda Pereira Dos Santos Silva, Allan David Silva, Sandro Tetsuo Emoto, Matheus Pereira-Santos, Jose Augusto Senhorini, Leandro Melo de Sousa, George Shigueki Yasui","doi":"10.1017/S0967199426100392","DOIUrl":"https://doi.org/10.1017/S0967199426100392","url":null,"abstract":"The objective of this study was to characterize macroscopically the female gametes, evaluate the survival rates, as well as describe in detail the embryonic development of Prosomyleus rhomboidalis. Three adult females from the Xingu River, presenting promptly ovulation were fertilized by the dry method using sperm from adult males. Egg incubation was set at 27.5°C, and oocyte characteristics and the main developmental stages were described. The results showed that the non-hydrated oocyte has an average diameter of 2.12 mm, yellow colour, spherical shape, is non-adhesive and the diameter increased after hydration at 0.36 mm, referring to the perivitelline space(PS). The fertilization rates (96.79 ± 9.52%) and hatching of normal larvae (96.21 ± 2.40%) were analysed, with hatching above 96%. After hydration of the eggs the PS arose and formed a visible animal pole ending with the hatching of the larvae 69 h and 39 min after fertilization. The description of developmental stages following fertilization was described for the first time in this species. The information presented in this work will serve as a basic support for new possibilities in conservation strategies, re-stocking and genebanking.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148057151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spermatogonial stem cell-based cellular therapy for non-obstructive azoospermia: a narrative review. 以精原干细胞为基础的细胞治疗非阻塞性无精子症：叙述性回顾。

IF 1.4 4区生物学

Zygote Pub Date : 2026-05-28 DOI: 10.1017/S0967199426100343

Maryam Salehi Novin, Mehdi Mehdinezhad Roshan, Omid Abouie Mehrizi, Keyvan Kiani, Hamed Hajipour

{"title":"Spermatogonial stem cell-based cellular therapy for non-obstructive azoospermia: a narrative review.","authors":"Maryam Salehi Novin, Mehdi Mehdinezhad Roshan, Omid Abouie Mehrizi, Keyvan Kiani, Hamed Hajipour","doi":"10.1017/S0967199426100343","DOIUrl":"https://doi.org/10.1017/S0967199426100343","url":null,"abstract":"Spermatogonial stem cells (SSCs) constitute the foundation of male sperm production and fertility. SSCs are characterised by their capacity for self-renewal and differentiation into spermatozoa throughout a male's reproductive life, thereby transmitting genetic information to subsequent generations. During embryonic development, SSCs are derived from postnatal testicular gonocytes, which originate from long-lived primordial germ cells. A large proportion of men's infertility is detected by sperm analysis. Unusual sperm parameters include sperm concentration, morphology and motility. Male infertility is mainly due to hormone deficits, physical causes, sexually transmitted diseases, environment, lifestyle and hereditary factors. The most severe form of male infertility is non-obstructive azoospermia (NOA), which is defined as no sperm in the ejaculate because of spermatogenesis failure. The first-line treatment for these individuals has been testicular sperm extraction under an operating microscope (micro-TESE). SSCs are also considered a promising pathway for regenerating impaired or damaged spermatogenesis. This review addresses SSC transplantation as a potential method for treating male infertility and restoring spermatogenesis in patients with degenerative diseases such as cancer, radiotherapy and chemotherapy.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-10"},"PeriodicalIF":1.4,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148044054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple method for several-week sperm preservation applicable for various echinoid species. 一种适用于多种棘皮类动物的简单精子保存方法。

IF 1.4 4区生物学

Zygote Pub Date : 2026-05-15 DOI: 10.1017/S0967199426100458

Sumio Udagawa, Yuuko Wada, Ryuta Yoshida, Masato Kiyomoto

{"title":"A simple method for several-week sperm preservation applicable for various echinoid species.","authors":"Sumio Udagawa, Yuuko Wada, Ryuta Yoshida, Masato Kiyomoto","doi":"10.1017/S0967199426100458","DOIUrl":"https://doi.org/10.1017/S0967199426100458","url":null,"abstract":"Easy, universal, and cost-effective methods of gamete preservation for echinoids are required since echinoids are important model organisms for studying fertilization and early development, and at the same time, they are valuable fishery resources, and key components of marine ecosystems. In general, cryopreservation is the common method of animal sperm preservation, but we previously reported a simple method using a chilled condition as an alternative choice, using Hemicentrotus pulcherrimus. In this study, we applied this method to various echinoid species to test whether it was effective for them, and then we determined the features of the preserved sperm in detail. The fertilization capability of preserved sperm was maintained for several weeks, though higher sperm density was needed to obtain 100% fertilization in later periods. Eggs fertilized by the preserved sperm developed normally, and the morphology of the embryos was not affected. Sperm swimming in a stable, regular manner were observed even at the end of the preservation period, though the flagellar beat frequency was substantially decreased during preservation. There was almost no change of the flagellar beat form during preservation. Here, we showed that our simple method is basically applicable to various echinoid species and provides the opportunity for effective and immediate use of competent echinoid sperm by a wide variety of users, such as people in research, fisheries and education.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-9"},"PeriodicalIF":1.4,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effective strategies for long-term bovine trophectoderm cell culture. 长期培养牛滋养外胚层细胞的成本效益策略。

IF 1.4 4区生物学

Zygote Pub Date : 2026-05-12 DOI: 10.1017/S096719942610046X

Ethel Sofia Moreno, Briza Castro, M Sofia Ortega

{"title":"Cost-effective strategies for long-term bovine trophectoderm cell culture.","authors":"Ethel Sofia Moreno, Briza Castro, M Sofia Ortega","doi":"10.1017/S096719942610046X","DOIUrl":"https://doi.org/10.1017/S096719942610046X","url":null,"abstract":"Long-term trophectoderm (TE) cell culture provides a powerful model to investigate placenta-specific factors to better understand mechanisms relevant to pregnancy establishment and placental development. However, current TE culture systems rely on costly commercial media and extracellular matrix (ECM) components, which limit their scalability and accessibility. This study evaluated cost-effective alternatives to established conditions by testing modified DMEM/F-12 and a biphasic TE culture system as substitutes for commercial Advanced DMEM/F-12 and for continuous TE culture, and by assessing 0.1% gelatine as an ECM alternative to collagen IV. Trophectoderm outgrowths cultured on collagen IV or gelatine did not differ in attachment timing (P = 0.78) or in expression of placental and differentiation markers PLAC8 (P = 0.78), GATA2 (P = 0.18), and PAG10 (P = 0.39). Similarly, blastocysts cultured in commercial Advanced DMEM/F-12 or base DMEM/F-12 exhibited no differences in attachment day (P = 0.98), TE growth area from days 10-20 (P > 0.05), or expression of PLAC8 (P = 0.35) and PAG10 (P = 0.08), although PAG7 differed between treatments (P < 0.05). Embryos cultured in continuous TE attached later (P = 0.01) than those cultured in TE media, but no differences were observed in TE growth area or expression of PLAC8, PAG7, or PAG10 (P > 0.05). Collectively, these results indicate that affordable media formulations and gelatine-coated cultureware support TE attachment, proliferation, and differentiation. This cost-effective culture framework enables broader application of TE models and supports extended studies of trophoblast function, placental signalling, and early conceptus development.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-7"},"PeriodicalIF":1.4,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147875947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel MOS variant causes large polar body formation and parthenogenetic activation; case report of an unusual phenomenon leading to female infertility. 一种新的MOS变异导致大极体形成和孤雌生殖激活；一例导致女性不孕症的异常现象报告。

IF 1.4 4区生物学

Zygote Pub Date : 2026-05-11 DOI: 10.1017/S0967199426100409

Serdar Coskun, Khalid Awartani, Rana Alhamdan, Islam Saadeldin, Asma Tahir, Sateesh Maddirevula

{"title":"A novel MOS variant causes large polar body formation and parthenogenetic activation; case report of an unusual phenomenon leading to female infertility.","authors":"Serdar Coskun, Khalid Awartani, Rana Alhamdan, Islam Saadeldin, Asma Tahir, Sateesh Maddirevula","doi":"10.1017/S0967199426100409","DOIUrl":"https://doi.org/10.1017/S0967199426100409","url":null,"abstract":"Oocyte maturation is regulated by well-orchestrated molecular mechanisms and any abnormalities during these events could result in an oocyte that cannot be fertilized or develop into normal embryos. Recently, MOS gene variants were reported to result in oocytes with large polar body (PB) or early embryonic arrest in humans leading to infertility. A thirty-year-old woman with 12 years of infertility underwent two in vitro fertilization cycles, and in both she had oocytes with large PBs and parthenogenetic activation. Clinical exome sequence was performed and revealed a novel homozygous variant in MOS (NM_005372.1):c.573C > A;p.(Phe191Leu). These findings might indicate that MOS mutation results in oocytes with a large PB and parthenogenesis in this patient. In conclusion, our data further confirms that MOS is important in proper oocyte maturation, and variant c 573 C > A results in the formation of a large PB and parthenogenetic activation as reported in animal models earlier. Patients with MOS variants need to be counselled about their fertility status.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-4"},"PeriodicalIF":1.4,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147869731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spermatogenesis depletion in diploid and triploid yellowtail tetra (Astyanax altiparanae) for germ cell transplantation. 二倍体和三倍体黄尾四鱼生殖细胞移植的精子发生缺失。

IF 1.4 4区生物学

Zygote Pub Date : 2026-04-16 DOI: 10.1017/S0967199426100380

Lucia Suarez Lopez, Rafaela Manchin Bertolini, Paulo Monzani, Gabriella Braga Carvalho, Diógenes Henrique de Siqueira-Silva, Jose Augusto Senhorini, Norberto Vianna, George Shigueki Yasui

{"title":"Spermatogenesis depletion in diploid and triploid yellowtail tetra (Astyanax altiparanae) for germ cell transplantation.","authors":"Lucia Suarez Lopez, Rafaela Manchin Bertolini, Paulo Monzani, Gabriella Braga Carvalho, Diógenes Henrique de Siqueira-Silva, Jose Augusto Senhorini, Norberto Vianna, George Shigueki Yasui","doi":"10.1017/S0967199426100380","DOIUrl":"https://doi.org/10.1017/S0967199426100380","url":null,"abstract":"The piracanjuba (Brycon orbignyanus) is a species threatened with extinction due to human activities, highlighting the need for genebanking and alternative propagation methods for its conservation. The objective of this study was to obtain a completely sterile yellowtail tetra (Astyanax altiparanae) and to transplant the stem spermatogonia (SSCs) of B. orbignyanus. Complete depletion of spermatogenesis in adult diploid males was successfully achieved through five applications of busulfan, using dosages of 15 mg kg-1 and 40 mg kg-1 at a high temperature of 35°C. One month after completing the busulfan treatment, spermatogenesis returned, occurring more rapidly in the 15 mg kg-1 treatment, with spermatozoa visible in the testicular lumen. In the treatment with 40 mg kg-1, only SSCs and some spermatid cysts were observed. Depletion of spermatogenesis in triploid juveniles was observed after two applications of busulfan (50 mg kg-1). One month after the end of the treatment, only a few SSCs cells were observed in the testes. The B. orbignyanus SSCs were isolated using a Percoll density gradient and identified by alkaline phosphatase activity, resulting in a cell suspension of 2.6 × 106 SSCs/mL with 93% cell viability. The cells were labeled with PKH26 and transplanted via the urogenital papilla into recipients (triploid juvenile males) previously treated with busulfan. PKH26-positive cells were visualized up to 80 days after transplantation. The combination of two sterilization methods appears to be more effective for cell transplantation after the endogenous germ cells have been eliminated from the recipient testes of triploid juveniles.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-14"},"PeriodicalIF":1.4,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal induction ensures the production of high-quality semen in fish compared to the presence of females from different production systems. 与来自不同生产系统的雌性相比，激素诱导可以确保鱼类产生高质量的精液。

IF 1.4 4区生物学

Zygote Pub Date : 2026-04-13 DOI: 10.1017/S0967199426100367

Antoni Manoel de Souza Cruz, Gabriel Pereira de Sousa, Laura Andrade de Moura, Eduardo Jorge Fernandes, Vinicius Rogério Leite, Edicarlos Pereira de Sousa, Raimundo Nonato Costa Ferreira, Cleonice Cristina Hilbig, George Shigueki Yasui, Nivaldo Ferreira do Nascimento

{"title":"Hormonal induction ensures the production of high-quality semen in fish compared to the presence of females from different production systems.","authors":"Antoni Manoel de Souza Cruz, Gabriel Pereira de Sousa, Laura Andrade de Moura, Eduardo Jorge Fernandes, Vinicius Rogério Leite, Edicarlos Pereira de Sousa, Raimundo Nonato Costa Ferreira, Cleonice Cristina Hilbig, George Shigueki Yasui, Nivaldo Ferreira do Nascimento","doi":"10.1017/S0967199426100367","DOIUrl":"https://doi.org/10.1017/S0967199426100367","url":null,"abstract":"This study investigated how hormonal induction, female presence, and production system affect sperm quality in Astyanax lacustris across three experiments. In Experiment 1, males and females were kept together in the same recirculating aquaculture system (RAS) before testing. Hormonal induction consistently boosted initial motility and prolonged sperm activity, while female presence offered only a modest benefit to non-induced males and no measurable effect when males were induced. Sperm concentration remained similar across treatments. Experiment 2 evaluated the same factors using broodstock originating from different rearing environments. When males and females came from separate RAS units, hormonal induction again sustained higher motility, and induced males paired with females showed higher sperm concentration than some non-induced groups. In the RAS-biofloc technology (BFT) pairing, hormonal induction maintained motility regardless of female presence; however, in the absence of hormonal induction, both sperm motility and concentration were modulated by the rearing system of females, with non-induced males paired with BFT females exhibiting lower reproductive performance. Overall, hormonal induction proved to be the most reliable strategy for ensuring high semen quality in A. lacustris. Nonetheless, when induction was not applied, male reproductive performance became more sensitive to female origin and rearing environment, highlighting the importance of broodstock compatibility and production system history in reproductive management protocols.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"1-6"},"PeriodicalIF":1.4,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147677161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene expression dynamics of TLR2 in twin- and single-bearing goats: a step toward molecular reproductive profiling. TLR2基因在双胎和单胎山羊中的表达动态：迈向分子生殖谱的一步。

IF 1.7 4区生物学

Zygote Pub Date : 2026-02-01 Epub Date: 2026-03-31 DOI: 10.1017/S096719942610032X

Ankit Magotra, Yogesh C Bangar, Sandeep Kumar, Devesh Kumar Yadav, Anil Bhat, Rohit Kumar

{"title":"Gene expression dynamics of TLR2 in twin- and single-bearing goats: a step toward molecular reproductive profiling.","authors":"Ankit Magotra, Yogesh C Bangar, Sandeep Kumar, Devesh Kumar Yadav, Anil Bhat, Rohit Kumar","doi":"10.1017/S096719942610032X","DOIUrl":"10.1017/S096719942610032X","url":null,"abstract":"This study investigated the expression profile of the Toll-like receptor 2 (TLR2) gene in goats across three reproductive groups - twin-bearing (n = 12), single-bearing (n = 10) and non-pregnant (n = 8) - using quantitative real-time PCR (qRT-PCR). Descriptive statistics revealed that twin-bearing goats were significantly heavier at service and kidding, with higher litter weights compared to other groups (p < 0.05). No breed-wise differences were observed, allowing pooled analysis for gene expression. Melt curve and amplification plot analyses confirmed primer specificity and efficient amplification. TLR2 expression was significantly upregulated in twin-bearing goats (ΔCt = -2.14 ± 0.89), with a mean 4.5-fold increase (p < 0.001) compared to non-pregnant controls, suggesting enhanced immune activation. Single-bearing goats showed negligible and non-significant changes in expression (ΔCt = -0.01 ± 1.45; p = 0.984). Statistical comparisons showed a significant difference in TLR2 expression between twin- and single-bearing groups (mean difference: 3.39; p < 0.001). These results reveals a significant association of twin pregnancies with elevated expression level of TLR2 gene in resource population. While the functional implications require further investigation, this upregulation may be associated with immune adaptations to increased gestational load. Overall, this study identifies TLR2 as a candidate gene expression marker strongly correlated with twin pregnancy in goats. Further validation through longitudinal studies on larger cohorts and investigation of tissue-specific expression is necessary to assess its potential predictive value for reproductive performance in small ruminants.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"12-19"},"PeriodicalIF":1.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147582242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

kdm4aa knockout disturbs early embryonic development in Zebrafish via downregulating rbm46. 敲除kdm4aa通过下调rbm46干扰斑马鱼早期胚胎发育。

IF 1.7 4区生物学

Zygote Pub Date : 2026-02-01 Epub Date: 2026-03-09 DOI: 10.1017/S0967199425100257

Hui Jiang, Xinqing Cai, Rui Deng, Jiamin Wang, Hongkuan Song, Weiran Chai, Bingshe Han, Junfang Zhang

{"title":"kdm4aa knockout disturbs early embryonic development in Zebrafish via downregulating rbm46.","authors":"Hui Jiang, Xinqing Cai, Rui Deng, Jiamin Wang, Hongkuan Song, Weiran Chai, Bingshe Han, Junfang Zhang","doi":"10.1017/S0967199425100257","DOIUrl":"10.1017/S0967199425100257","url":null,"abstract":"Histone lysine-specific demethylase 4A (KDM4A) plays a critical role in the embryonic development of mammals such as mouse and goat, however its function in zebrafish (Danio rerio) embryogenesis remains poorly understood, due to the existence of two kdm4a paralogs (kdm4aa and kdm4ab) in zebrafish. The current study revealed that kdm4aa-/- embryos exhibited dramatically increased mortality during gastrulation. RT-qPCR showed that RNA-binding motif protein 46 (rbm46) was downregulated after kdm4aa knockout. CUT&Tag-qPCR revealed that kdm4aa knockout significantly decreased H3K4me3, while increasing H3K9me3 and H3K36me3 at rbm46 promoter. kdm4aa-/- embryos displayed elevated reactive oxygen species (ROS) and reduced adenosine triphosphate (ATP). And rbm46 mRNA injection alleviated ROS accumulation and increased ATP level, thereby rescuing the lethal phenotype in kdm4aa-/- embryos. Our findings demonstrate that kdm4aa knockout disturbs zebrafish embryogenesis by suppressing rbm46 mRNA expression.","PeriodicalId":24075,"journal":{"name":"Zygote","volume":" ","pages":"29-39"},"PeriodicalIF":1.7,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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