Zoonoses and Public Health最新文献

{"title":"Spatiotemporal Early Warning of Human Brucellosis in Hubei Province Using Coupled Ecological-Time Series Models","authors":"Qi Chen,&nbsp;Buasiyamu Abudunaibi,&nbsp;Xiaoying Zhao,&nbsp;Jiangshaya Bahati,&nbsp;Yao Wang,&nbsp;Jia Rui,&nbsp;Xuhua Guan,&nbsp;Tianmu Chen","doi":"10.1111/zph.70046","DOIUrl":"10.1111/zph.70046","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>This study explored transmission patterns of brucellosis in Hubei Province, a Class II region in China, through analysis of spatiotemporal distribution and environmental factors to provide evidence for precise prevention.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We analysed epidemiological data from Hubei Province (2010–2023), including human cases, demographics, livestock numbers and environmental factors. The MaxEnt model was used for spatial risk prediction, while the seasonal autoregressive integrated moving average (SARIMA) model analysed temporal patterns and warning effectiveness.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Human brucellosis showed a ‘rapid rise-effective control-local rebound’ pattern, with incidence rates rising from 0.0087/100,000 (2010) to 0.5932/100,000 (2015), declining to 0.1535/100,000 (2019) and rebounding to 0.4508/100,000 (2023). The epidemic displayed seasonal peaks (April–September) and affected primarily males (71.13%) and farmers (76.54%). Spatial distribution showed a ‘high-north low-south’ pattern, spreading from Suizhou to neighbouring areas. The MaxEnt model identified high-risk areas (probability ≥ 0.713) in livestock-intensive regions, with sheep density (55.1%) and annual mean temperature (9.9%) as key factors. The SARIMA model indicated earliest warnings in high-risk areas (mid-late April), followed by medium-risk (early-mid April) and low-risk areas (late May).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Brucellosis transmission in Hubei is influenced by the livestock industry, control measures and climate. The integrated MaxEnt-SARIMA model provides risk classification and differentiated warning schemes, offering guidance for targeted prevention across different risk areas.</p>\u0000 </section>\u0000 </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"73 3","pages":"244-261"},"PeriodicalIF":2.3,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13053619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147311046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One Health Genomic Analysis of Methicillin-Resistant Staphylococcus spp. From Humans, Cats, and Dogs in a Veterinary Hospital in Central Chile","authors":"Daniel Garrido,&nbsp;Danny Fuentes-Castillo,&nbsp;Leslye Camila del Río,&nbsp;Jorge Barraza-Schulmeyer,&nbsp;Felipe Melis,&nbsp;Patricia García,&nbsp;Carla Flores-Yáñez,&nbsp;Pamela Thomson","doi":"10.1111/zph.70042","DOIUrl":"10.1111/zph.70042","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>The emergence of methicillin-resistant <i>Staphylococcus</i> species (MRS) poses a growing threat to both human and veterinary health.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We performed a comparative genomic characterisation of 49 methicillin-resistant <i>Staphylococcus</i> isolates collected from humans, dogs, and cats in a veterinary hospital from Central Chile.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Whole-genome sequencing revealed <i>S. aureus</i>, <i>S. pseudintermedius</i>, and <i>S. epidermidis</i> as the most prevalent species, with distinct patterns of antimicrobial resistance and virulence. Average Nucleotide Identity (ANI) and phylogenetic analyses highlighted species-specific clustering but also revealed divergent lineages among <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> isolates. Resistance to β-lactams was widespread, with the <i>blaZ</i> and <i>mecA</i> genes predominating, while macrolide and aminoglycoside resistance genes were variably distributed. <i>S. aureus</i> exhibited the highest virulence gene content, whereas <i>S. pseudintermedius</i> isolates carried superantigen-encoding enterotoxin genes, suggesting potential zoonotic risk. MLST and SCCmec typing confirmed the circulation of community-associated MRS lineages, notably SCCmec type IV.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study provides a first comprehensive characterisation of methicillin-resistant <i>Staphylococcus</i> spp. isolates from human and animal hosts at a veterinary hospital in Santiago, Chile. These findings underscore the need for continuous surveillance under the One Health approach to monitor the potential transmission of high-risk clones and resistance determinants between humans and companion animals.</p>\u0000 </section>\u0000 </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"73 3","pages":"205-217"},"PeriodicalIF":2.3,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13053636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146195561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Molecular Detection of the Potential Zoonotic Pathogen Rickettsia asembonensis in Client-Owned Dogs and Cats in Thailand","authors":"Aung Aung,&nbsp;Kritsada Thongmeesee,&nbsp;Wittawat Wechtaisong,&nbsp;Chalida Sri-in,&nbsp;Thuong Thi Huyen Bui,&nbsp;Sonthaya Tiawsirisup","doi":"10.1111/zph.70048","DOIUrl":"10.1111/zph.70048","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Rickettsioses are predominantly zoonotic infections that circulate among animal populations and can be transmitted to humans, representing emerging vector-borne diseases spread through infected arthropod vectors. <i>Rickettsia asembonensis</i> displays a broad geographic distribution and is predominantly associated with fleas. Recent reports have identified <i>R</i>. <i>asembonensis</i> in febrile human patients in Malaysia, Peru, and Zambia, underscoring its emerging zoonotic potential. In Thailand, however, investigations of <i>Rickettsia</i> spp. have concentrated mainly on ticks and fleas, with comparatively little information regarding infections in pet dogs and cats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The present study examined <i>Rickettsia</i> spp. in 968 pets, 472 client-owned dogs, and 496 client-owned cats from the Bangkok and Nakhon Pathom regions, using the <i>17-kDa</i> gene as a molecular marker. Genetic analyses of <i>Rickettsia</i> species were performed using maximum-likelihood phylogeny with IQ-TREE, Bayesian inference, pairwise sequence similarity assessment, and nucleotide sequence type network analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 114 pets tested positive, corresponding to a prevalence of 11.8%, with 21.6% (102/472) in dogs and 2.4% (12/496) in cats. Of these, 48 samples were sequenced, leading to the identification of <i>R</i>. <i>asembonensis</i> and either uncultured <i>Rickettsia</i> spp. or the <i>Rickettsia</i> endosymbiont of <i>Haemaphysalis bispinosa</i>. Statistical analysis indicated significantly higher infection rates in pet dogs and cats from the Nakhon Pathom region. Phylogenetic reconstruction with maximum-likelihood and Bayesian-inference approaches revealed distinct clustering of <i>R</i>. <i>asembonensis</i> and uncultured <i>Rickettsia</i> spp. Furthermore, three nucleotide sequence types of <i>R</i>. <i>asembonensis</i> identified here were unique and not shared with isolates from other countries or host species, as demonstrated by phylogenetic and Templeton–Crandall–Sing network analyses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This work provides the first documentation of <i>R</i>. <i>asembonensis</i> in client-owned urban dogs and cats in Thailand and highlights the need for increased awareness among veterinarians and pet owners, given its public health relevance.</p>\u0000 </section>\u0000 </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"73 3","pages":"262-275"},"PeriodicalIF":2.3,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147366863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crimean-Congo Haemorrhagic Fever Virus Exposure in Horses and Donkeys in Border Livestock Markets of Nigeria.","authors":"David O Ehizibolo, Olumuyiwa Oyekan, Nicodemus Mkpuma, Habibu Haliru, Ibrahim Garba, Isa Z Turaki, Samdi Kennedy, Joshua M Shallangwa, Caleb S Kilyobas, Abdullahi Mohammed, Musa A Muhammad, Mansur Abubakar, Maryam Muhammad, Corrie Brown, Bonto Faburay","doi":"10.1111/zph.70056","DOIUrl":"https://doi.org/10.1111/zph.70056","url":null,"abstract":"<p><strong>Introduction: </strong>Crimean-Congo haemorrhagic fever virus (CCHFV) is a tick-borne zoonotic pathogen of major public health importance in Africa. Although CCHFV exposure has been reported in several livestock species in Nigeria, data on equids remain limited despite their extensive involvement in livestock movement and trade. This study assessed CCHFV seroprevalence in horses and donkeys in major international livestock markets in northern Nigeria and examined factors associated with exposure.</p><p><strong>Methods: </strong>From June 2023 to July 2024, a longitudinal sero-surveillance study was conducted in three high-volume livestock markets in northern Nigeria. Serum samples from 808 equids (305 horses and 503 donkeys) were tested for CCHFV-specific IgG antibodies using a commercial multi-species ELISA. Data on age, sex, origin and tick infestation status were collected. Seroprevalence was estimated with 95% confidence intervals, and associations were evaluated using univariable analyses and multilevel binomial logistic regression accounting for market-state clustering.</p><p><strong>Results: </strong>Overall CCHFV seroprevalence was 23.1% (95% CI: 20.3%-26.2%). Horses had a significantly higher seroprevalence (52.1%, 95% CI: 46.3%-58.0%) than donkeys (5.7%, 95% CI: 3.7%-8.1%) (p < 0.001). In univariable analyses, seropositivity in horses was associated with age and sex, whereas no significant associations were observed in donkeys. In the multilevel model, species and age remained independently associated with seropositivity; horses had substantially higher odds than donkeys (OR = 20.91, 95% CI: 11.14-39.24), and odds increased with age (OR = 1.17 per year, 95% CI: 1.06-1.29).</p><p><strong>Conclusions: </strong>CCHFV exposure is widespread among equids in northern Nigeria, particularly in horses. These findings support the potential value of horses as sentinel species for CCHFV surveillance in livestock trade corridors.</p><p><strong>Summary: </strong>Crimean-Congo haemorrhagic fever virus (CCHFV) is a tick-borne zoonotic virus of public health importance in Africa, yet data on equids in Nigeria remain limited. We assessed CCHFV seroprevalence in horses and donkeys sampled at major international livestock markets in northern Nigeria. Serum samples from 808 equids were tested using a commercial multi-species ELISA. Overall seroprevalence was 23.1% (95% CI: 20.3%-26.2%), with higher seroprevalence in horses (52.1%) than in donkeys (5.7%). In multilevel analyses, species and age were independently associated with seropositivity, while sex, tick infestation status and animal origin were not. These findings indicate widespread CCHFV exposure among equids and suggest that horses may serve as useful sentinel animals for monitoring CCHFV circulation in livestock trade corridors.</p>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Sporadic Cases of Erysipelothrix rhusiopathiae Infection, Minnesota 2024.","authors":"Kelly E Schenk, Jeffrey Kubiak, Jennifer L Dale, Nate Wright, Jemee Moua, Paula Snippes Vagnone, Patricia Ferrieri, Malia Ireland, Kirk Smith, Stacy Holzbauer","doi":"10.1111/zph.70053","DOIUrl":"https://doi.org/10.1111/zph.70053","url":null,"abstract":"<p><strong>Introduction: </strong>Erysipelothrix rhusiopathiae is a gram-positive, rod-shaped bacterium that can cause infections in livestock, marine mammals and humans. Human infections are traditionally affiliated with occupational exposures to E. rhusiopathiae through farming, butchering, or cooking. In July 2024, the Minnesota Department of Health was notified of two E. rhusiopathiae infections in the Minneapolis-St. Paul metropolitan area. Concerned about a possible outbreak, an investigation was initiated to determine the possible source(s) of infection.</p><p><strong>Methods: </strong>Epidemiologists conducted reiterative, semi-structured interviews with the families of each case-patient. Cultures were forwarded to the MDH Public Health Laboratory for next-generation sequencing to determine the relatedness of the two samples, and additional queries of electronic health records (EHRs) were utilised to detect any additional cases of E. rhusiopathiae occurring in the Minneapolis-St. Paul area.</p><p><strong>Results: </strong>Both case-patients were adults from the Hmong community with underlying health conditions that presented to emergency care on two separate occasions. Interviews with the case-patients' families elucidated that they may have had direct or indirect exposures to raw or undercooked meat, possibly from a live animal market, but otherwise had no similar exposures. At MDH-PHL, the isolates were confirmed as E. rhusiopathiae and were noted to be 23,328 single nucleotide polymorphisms different. No additional case-patients were identified through a query of EHRs.</p><p><strong>Conclusions: </strong>Given the lack of an epidemiological linkage and no apparent genetic relatedness of these two samples, epidemiologists considered these cases to likely not be part of an outbreak. While the true source of infection for these cases could not be determined, interviews helped elucidate possible exposure routes of infection, including contact with undercooked meat or environmental exposure to surfaces contaminated by raw meat. Given its rarity, E. rhusiopathiae cases should be studied when they occur to establish greater epidemiological and molecular evidence to support future outbreak investigations of the pathogen.</p>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147436091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial—Introducing Zoonoses and Public Health's New Co-Editors in Chief for 2026","authors":"Christopher Hamilton-West,&nbsp;Angela Fanelli","doi":"10.1111/zph.70035","DOIUrl":"10.1111/zph.70035","url":null,"abstract":"","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"73 2","pages":"113-114"},"PeriodicalIF":2.3,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The First Biological Validation of Taenia solium Risk Assessment Tools","authors":"Andrew Larkins,&nbsp;Davina Boyd,&nbsp;Oula Bouphakaly,&nbsp;Bounnaloth Insisiengmay,&nbsp;Malavanh Chittavong,&nbsp;Amanda Ash","doi":"10.1111/zph.70023","DOIUrl":"10.1111/zph.70023","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p><i>Taenia solium</i> places substantial burden on communities in low and middle-income countries, where neurocysticercosis is a leading cause of preventable epilepsy. Current diagnostic tests for <i>T. solium</i> are not suited for low resource settings or perform poorly. Several risk-assessment tools have been developed to support countries in identifying areas for further <i>T. solium</i> investigation and intervention. These tools are based on risk, rather than disease data, and have yet to be validated against biological results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Two national quantitative risk-assessment tools (LISA and MCDA), two local semi-quantitative tools (rapid and in-depth), and a local qualitative tool (workshop) had their performance assessed against biological <i>T. solium</i> taeniasis data from 28 villages. The final risk category, risk score and individual components of each tool were assessed independently against village <i>T. solium</i> status by logistic regression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><i>T. solium</i> positive villages had higher odds of being high-risk villages for all tools, however, this was only significant for the LISA and workshop tools. For the LISA tool, the median risk score was also significantly greater in positive villages. A unit increase in LISA risk score led to a significant increase in the odds of a village being positive for <i>T. solium</i>. Other tools that calculated risk scores also showed similar results, however, were not statistically significant. All positive villages were hotspots for unimproved toilets by the LISA tool. The open defaecation component of the local rapid tool was the only component of any tool to demonstrate a significant relationship with positive <i>T. solium</i> villages.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This first biological validation of <i>T. solium</i> risk-assessment tools demonstrates that there are multiple tools that should be considered for further development. Supporting endemic countries to implement recently developed risk-assessment tools is consistent with the World Health Organization's goal of intensified control of <i>T. solium</i> in hyperendemic areas.</p>\u0000 </section>\u0000 </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"73 2","pages":"115-127"},"PeriodicalIF":2.3,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145991057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosecurity Uptake and Perceived Risk of Avian Influenza Among People in Contact With Birds","authors":"Harry Whitlow,&nbsp;Suzanne Gokool,&nbsp;Genevieve Clapp,&nbsp;Irene Bueno,&nbsp;Mariam Logunleko,&nbsp;Peter Moore,&nbsp;Sarah Masterton,&nbsp;Jo Taylor-Egbeyemi,&nbsp;Ian Brown,&nbsp;Riinu Pae,&nbsp;Louise E. Smith,&nbsp;Ellen Brooks-Pollock,&nbsp;Amy C. Thomas","doi":"10.1111/zph.70034","DOIUrl":"10.1111/zph.70034","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Recent intercontinental spread of highly pathogenic avian influenza (HPAI) A(H5N1) among kept and wild birds, and transmission to mammalian hosts, including cattle and humans, has heightened the need to review public health risk assessments. Biosecurity measures (BMs) are essential for limiting disease spread, but how widely different practices are implemented is not fully known.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Here, we report on the uptake of BMs and risk perception of avian influenza virus (AIV) in the context of preventing zoonotic transmission to persons potentially at high risk of exposure. Questionnaire data from 225 people in contact with birds in the UK (Avian Contact Study, May to July 2024) were analysed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We found hand washing after contact with birds was the most common BM implemented (89%, 196 of 218), followed by using disinfecting footwear dips (78%, 170 of 218). Individuals in contact with a higher number of birds were more likely to use at least one personal protective equipment (PPE) measure for the face or body or at least one footwear-related PPE measure. Perceived risk of AIV to bird health was high for individuals in contact with large flocks (≥ 1001 birds) and associated with uptake of at least one footwear-related PPE measure (independent of flock size). Perceived risk of AIV to respondents' own health was low, regardless of the number of birds a respondent had daily contact with.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our results indicate that routinely used BMs are implemented to limit AIV spread among birds, but not with the purpose of limiting zoonotic transmission from birds to humans. Identifying cohort characteristics which could lead to low BM uptake, alongside barriers and facilitators to BM uptake, is important for informing zoonotic AIV public health campaigns.</p>\u0000 </section>\u0000 </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"73 2","pages":"152-163"},"PeriodicalIF":2.3,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12865334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145913241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"‘Bites of Knowledge’: Ticks and Tick-Borne Pathogens Unveiled Through a Citizen Science Programme in Northern Spain","authors":"Javier Millán,&nbsp;Ruth Rodríguez-Pastor,&nbsp;Clara Muñoz-Hernández,&nbsp;Marta Sánchez-Sánchez,&nbsp;Alberto Moraga-Fernández,&nbsp;Natalia Fernández-Ruiz,&nbsp;Isabel G. Fernández de Mera,&nbsp;Agustín Estrada-Peña","doi":"10.1111/zph.70030","DOIUrl":"10.1111/zph.70030","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>We present the findings of a citizen science project conducted in the Autonomous Region of Aragón (northern Spain), which engaged rural schoolchildren, wildlife rangers, hunters, and members of the general public.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Over the course of one year (April 2022–April 2023; three months for the schoolchildren), participants collected a total of 913 ticks (98.8% adult specimens) representing 13 species from across the region. The contributions included 373 ticks collected by schoolchildren, 319 by hunters, 108 by rangers, 91 by other volunteers, and 22 by the research team. Of these, 54 ticks were gathered from vegetation, 422 from wildlife (spanning nine animal species), 362 from pets, 44 from humans, and 30 from livestock.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Species identified were <i>Rhipicephalus sanguineus</i> s.l.<i>, Rhipicephalus sanguineus</i> s.s.<i>, Rhipicephalus pusillus, Rhipicephalus bursa, Dermacentor marginatus, Ixodes hexagonus, Ixodes ricinus, Ixodes frontalis, Haemaphysalis punctata, Haemaphysalis sulcata, Haemaphysalis inermis, Hyalomma marginatum,</i> and <i>Hyalomma lusitanicum</i>. DNA of a subset of 349 individual ticks was extracted and the presence of <i>Rickettsia</i> spp., Anaplasmataceae, and <i>Borrelia burgdorferi</i> sensu lato (the latter, only in <i>Ixodes</i> spp.) was analysed either individually (<i>n</i> = 62) or in pools containing 2–5 ticks (<i>n</i> = 79), of which 101 resulted positive: 88 were positive for <i>Rickettsia</i> spp., revealing the presence of <i>Rickettsia massiliae</i>, <i>Rickettsia slovaca, Rickettsia conorii</i> subsp. <i>raoultii, Rickettsia aeschlimannii</i>, <i>Rickettsia hoogstraalii, Rickettsia helvetica,</i> and <i>Candidatus</i> Rickettsia barbariae; 34 for Anaplasmataceae (both <i>Ehrlichia</i> spp. and <i>Anaplasma</i> spp., but in many cases was identified as endosymbionts); and three for <i>B. burgdorferi</i> s.l., with only one readable sequence (<i>Borrelia valaisiana</i>). Pathogens detected in ticks from humans included <i>R. massiliae</i> (<i>n</i> = 3), <i>R. conorii raoultii, R. aeschlimannii</i> and <i>Ca</i>. R. barbariae.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>This study identified previously unreported tick-host-pathogen associations and contributed to raising awareness about the public health significance of ticks and strategies for their prevention.</p>\u0000 </section>\u0000 </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"73 2","pages":"137-151"},"PeriodicalIF":2.3,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145670152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Genome Sequencing Analysis Revealed High Genetic Diversity and Drug-Resistant Characteristics of Mycobacterium bovis From Chinese Farms","authors":"Xinyue Li,&nbsp;Yuhui Dong,&nbsp;Ruida Xing,&nbsp;Dan Su,&nbsp;Bing Zhao,&nbsp;Shaojun Pei,&nbsp;Xiaolong Cao,&nbsp;Ruiqing Zhang,&nbsp;Jiangyong Zeng,&nbsp;Dongjing Wang,&nbsp;Weixing Fan,&nbsp;Hui Xia,&nbsp;Shengfen Wang,&nbsp;Xichao Ou,&nbsp;Yanlin Zhao","doi":"10.1111/zph.70026","DOIUrl":"10.1111/zph.70026","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Bovine tuberculosis (bTB) has a certain incidence on farms in China, but little is known about its prevalence and the pathogenic <i>Mycobacterium</i> species. With the increasing application of whole-genome sequencing (WGS), it is now possible to investigate the genomic diversity, lineage distribution, and drug resistance profiles of <i>M. bovis</i> at high resolution.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this study, we sequenced 29 <i>Mycobacterium tuberculosis</i> complex (MTBC) isolates from two farms in China and incorporated genomic data from 79 publicly available <i>M. bovis</i> isolates from cattle farms in China for a comprehensive genomic analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The majority of the 106 <i>M. bovis</i> isolates (63.2%, 67/106) belong to lineage La1.4. The dataset was screened for isolates of two known clonal complexes, Eu1 and Eu3, corresponding to La1.8.1 and La1.2, respectively. In silico spoligotyping revealed nine previously identified profiles, and the most common spoligotype in this study is SB1903. All isolates have the H57D mutation in the <i>pncA</i> gene associated with pyrazinamide (PZA) resistance. 16.0% (17/106) of isolates have resistance mutations to first- or second-line drugs other than PZA, with one isolate classified as pre-XDR-TB. The distribution of cluster strains suggests the possibility of recent transmission and outbreak in the two farms. It is noted that two <i>M. tuberculosis</i> isolates from tissue samples had detectable drug resistance mutations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The results of this study provide the basis for in-depth analysis of genomic population characteristics and drug resistance of <i>M. bovis</i> in China. It should be noted that close contact between humans and cattle increases the risk of <i>M. tuberculosis</i> transmission from humans to cattle in rural areas or developing countries with low levels of human-animal separation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"73 2","pages":"128-136"},"PeriodicalIF":2.3,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145670101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}