Chavin Leelapsawas, Rungtip Chuanchuen, Parinya Sroithongkham, Vincent Perreten, Pattrarat Chanchaithong
{"title":"来自伴侣动物的产金属β-内酰胺酶高风险铜绿假单胞菌ST235和ST654的新型1类整合子结构","authors":"Chavin Leelapsawas, Rungtip Chuanchuen, Parinya Sroithongkham, Vincent Perreten, Pattrarat Chanchaithong","doi":"10.1111/zph.13224","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>Carbapenemase-producing <i>Pseudomonas aeruginosa</i> (CP-<i>Pa</i>) has emerged as a significant clinical and public health concern due to its ability to limit treatment options with last-resort antimicrobials. This study aims to characterise novel class 1 integron (Int1) structures containing <i>bla</i><sub>VIM-2</sub> and <i>bla</i><sub>IMP-1</sub> in high-risk CP-<i>Pa</i> sequence type (ST) 235 and ST654 strains from dogs and cats and illustrate the genetic relatedness between CP-<i>Pa</i> strains from animal and human origins.</p>\n </section>\n \n <section>\n \n <h3> Methods and Results</h3>\n \n <p>Of the four CP-<i>Pa</i> strains, whole-genome sequencing and analysis revealed that three strains belonged to ST235/O11/<i>exoU+</i>/<i>exoS-</i>, with two strains harbouring <i>bla</i><sub>VIM-2</sub>, and one strain harbouring <i>bla</i><sub>IMP-1</sub>. The remaining strain was characterised as ST654/O4/<i>exoU-</i>/<i>exoS+</i> and harboured <i>bla</i><sub>VIM-2</sub>. Core-genome single nucleotide polymorphism-based phylogeny illustrated genetic relationships between animal and human high-risk CP-<i>Pa</i> ST235/O11 and ST654/O4 strains within the same STs. Three novel Int1 gene cassette arrays were identified in the canine and feline CP-<i>Pa</i> strains in this study. The novel ~6.2-kb <i>bla</i><sub>VIM-2</sub>-containing Int1[Pae-CUVET21-397] and Int1[Pae-CUVET23-830] (<i>intI1</i>-<i>aadB</i>-<i>bla</i><sub>VIM-2</sub>-<i>aadB</i>-<i>cmlA6</i>-<i>qacE</i>∆<i>1</i>-<i>folP</i>) formed a complex Int1 located in an integrative and conjugative element of two feline CP-<i>Pa</i> ST235 strains. The canine CP-<i>Pa</i> ST235 strain CUVET20-956 contained a novel ~5.8-kb <i>bla</i><sub>IMP-1</sub>-containing Int1[Pae-CUVET20-956] (<i>intI1</i>-<i>bla</i><sub>IMP-1</sub>-<i>aacA4</i>-<i>aacA4</i>-IS<i>1595</i>-<i>qacE</i>∆<i>1</i>-<i>sul1</i>). The CP-<i>Pa</i> ST654 strain CUVET18-860 contained In<i>1206</i> encoding <i>bla</i><sub>VIM-2</sub> and a novel ~5.9-kb Int1[Pae-CUVET18-860] (∆<i>intI1</i>-<i>bla</i><sub>VEB-1</sub>-<i>aadB</i>-<i>qnrVC1</i>-<i>aacA4</i>-<i>bla</i><sub>OXA-10</sub>-<i>aadA1</i>-<i>dfrA14</i>) encoding multidrug resistance (MDR). Additionally, In<i>51</i>-containing Tn<i>6162</i> inserted in genomic island 1 was identified in all CP-<i>Pa</i> ST235 strains.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The novel Int1s encoding metallo-β-lactamases and MDR in canine and feline CP-<i>Pa</i> play a crucial role in the development of resistance to multiple antimicrobials and last-resort carbapenems in <i>P. aeruginosa</i> ST235 and ST654 strains of veterinary and public health importance, posing possible zoonotic and anthroponotic transmission of the high-risk CP-<i>Pa</i> clones between companion animals and humans.</p>\n </section>\n </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"72 5","pages":"453-464"},"PeriodicalIF":2.3000,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Novel Class 1 Integron Structures in High-Risk Metallo-β-Lactamase-Producing Pseudomonas aeruginosa ST235 and ST654 From Companion Animals\",\"authors\":\"Chavin Leelapsawas, Rungtip Chuanchuen, Parinya Sroithongkham, Vincent Perreten, Pattrarat Chanchaithong\",\"doi\":\"10.1111/zph.13224\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Introduction</h3>\\n \\n <p>Carbapenemase-producing <i>Pseudomonas aeruginosa</i> (CP-<i>Pa</i>) has emerged as a significant clinical and public health concern due to its ability to limit treatment options with last-resort antimicrobials. This study aims to characterise novel class 1 integron (Int1) structures containing <i>bla</i><sub>VIM-2</sub> and <i>bla</i><sub>IMP-1</sub> in high-risk CP-<i>Pa</i> sequence type (ST) 235 and ST654 strains from dogs and cats and illustrate the genetic relatedness between CP-<i>Pa</i> strains from animal and human origins.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods and Results</h3>\\n \\n <p>Of the four CP-<i>Pa</i> strains, whole-genome sequencing and analysis revealed that three strains belonged to ST235/O11/<i>exoU+</i>/<i>exoS-</i>, with two strains harbouring <i>bla</i><sub>VIM-2</sub>, and one strain harbouring <i>bla</i><sub>IMP-1</sub>. The remaining strain was characterised as ST654/O4/<i>exoU-</i>/<i>exoS+</i> and harboured <i>bla</i><sub>VIM-2</sub>. Core-genome single nucleotide polymorphism-based phylogeny illustrated genetic relationships between animal and human high-risk CP-<i>Pa</i> ST235/O11 and ST654/O4 strains within the same STs. Three novel Int1 gene cassette arrays were identified in the canine and feline CP-<i>Pa</i> strains in this study. The novel ~6.2-kb <i>bla</i><sub>VIM-2</sub>-containing Int1[Pae-CUVET21-397] and Int1[Pae-CUVET23-830] (<i>intI1</i>-<i>aadB</i>-<i>bla</i><sub>VIM-2</sub>-<i>aadB</i>-<i>cmlA6</i>-<i>qacE</i>∆<i>1</i>-<i>folP</i>) formed a complex Int1 located in an integrative and conjugative element of two feline CP-<i>Pa</i> ST235 strains. The canine CP-<i>Pa</i> ST235 strain CUVET20-956 contained a novel ~5.8-kb <i>bla</i><sub>IMP-1</sub>-containing Int1[Pae-CUVET20-956] (<i>intI1</i>-<i>bla</i><sub>IMP-1</sub>-<i>aacA4</i>-<i>aacA4</i>-IS<i>1595</i>-<i>qacE</i>∆<i>1</i>-<i>sul1</i>). The CP-<i>Pa</i> ST654 strain CUVET18-860 contained In<i>1206</i> encoding <i>bla</i><sub>VIM-2</sub> and a novel ~5.9-kb Int1[Pae-CUVET18-860] (∆<i>intI1</i>-<i>bla</i><sub>VEB-1</sub>-<i>aadB</i>-<i>qnrVC1</i>-<i>aacA4</i>-<i>bla</i><sub>OXA-10</sub>-<i>aadA1</i>-<i>dfrA14</i>) encoding multidrug resistance (MDR). Additionally, In<i>51</i>-containing Tn<i>6162</i> inserted in genomic island 1 was identified in all CP-<i>Pa</i> ST235 strains.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>The novel Int1s encoding metallo-β-lactamases and MDR in canine and feline CP-<i>Pa</i> play a crucial role in the development of resistance to multiple antimicrobials and last-resort carbapenems in <i>P. aeruginosa</i> ST235 and ST654 strains of veterinary and public health importance, posing possible zoonotic and anthroponotic transmission of the high-risk CP-<i>Pa</i> clones between companion animals and humans.</p>\\n </section>\\n </div>\",\"PeriodicalId\":24025,\"journal\":{\"name\":\"Zoonoses and Public Health\",\"volume\":\"72 5\",\"pages\":\"453-464\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-05-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zoonoses and Public Health\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/zph.13224\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoonoses and Public Health","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/zph.13224","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Novel Class 1 Integron Structures in High-Risk Metallo-β-Lactamase-Producing Pseudomonas aeruginosa ST235 and ST654 From Companion Animals
Introduction
Carbapenemase-producing Pseudomonas aeruginosa (CP-Pa) has emerged as a significant clinical and public health concern due to its ability to limit treatment options with last-resort antimicrobials. This study aims to characterise novel class 1 integron (Int1) structures containing blaVIM-2 and blaIMP-1 in high-risk CP-Pa sequence type (ST) 235 and ST654 strains from dogs and cats and illustrate the genetic relatedness between CP-Pa strains from animal and human origins.
Methods and Results
Of the four CP-Pa strains, whole-genome sequencing and analysis revealed that three strains belonged to ST235/O11/exoU+/exoS-, with two strains harbouring blaVIM-2, and one strain harbouring blaIMP-1. The remaining strain was characterised as ST654/O4/exoU-/exoS+ and harboured blaVIM-2. Core-genome single nucleotide polymorphism-based phylogeny illustrated genetic relationships between animal and human high-risk CP-Pa ST235/O11 and ST654/O4 strains within the same STs. Three novel Int1 gene cassette arrays were identified in the canine and feline CP-Pa strains in this study. The novel ~6.2-kb blaVIM-2-containing Int1[Pae-CUVET21-397] and Int1[Pae-CUVET23-830] (intI1-aadB-blaVIM-2-aadB-cmlA6-qacE∆1-folP) formed a complex Int1 located in an integrative and conjugative element of two feline CP-Pa ST235 strains. The canine CP-Pa ST235 strain CUVET20-956 contained a novel ~5.8-kb blaIMP-1-containing Int1[Pae-CUVET20-956] (intI1-blaIMP-1-aacA4-aacA4-IS1595-qacE∆1-sul1). The CP-Pa ST654 strain CUVET18-860 contained In1206 encoding blaVIM-2 and a novel ~5.9-kb Int1[Pae-CUVET18-860] (∆intI1-blaVEB-1-aadB-qnrVC1-aacA4-blaOXA-10-aadA1-dfrA14) encoding multidrug resistance (MDR). Additionally, In51-containing Tn6162 inserted in genomic island 1 was identified in all CP-Pa ST235 strains.
Conclusions
The novel Int1s encoding metallo-β-lactamases and MDR in canine and feline CP-Pa play a crucial role in the development of resistance to multiple antimicrobials and last-resort carbapenems in P. aeruginosa ST235 and ST654 strains of veterinary and public health importance, posing possible zoonotic and anthroponotic transmission of the high-risk CP-Pa clones between companion animals and humans.
期刊介绍：
Zoonoses and Public Health brings together veterinary and human health researchers and policy-makers by providing a venue for publishing integrated and global approaches to zoonoses and public health. The Editors will consider papers that focus on timely collaborative and multi-disciplinary research in zoonoses and public health. This journal provides rapid publication of original papers, reviews, and potential discussion papers embracing this collaborative spirit. Papers should advance the scientific knowledge of the sources, transmission, prevention and control of zoonoses and be authored by scientists with expertise in areas such as microbiology, virology, parasitology and epidemiology. Articles that incorporate recent data into new methods, applications, or approaches (e.g. statistical modeling) which enhance public health are strongly encouraged.
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