Veterinary ophthalmology最新文献

{"title":"Treatment outcome of spontaneous chronic corneal epithelial defects in brachycephalic dogs comparing Boxers and non-Boxers: A retrospective multicenter study of 420 dogs.","authors":"Catarina A V Gonçalves, James A C Oliver, Christiane Kafarnik, Kyle J Garnett, James O Rushton, Vida Stravinskaitė, Richard Everson, Francesca Tee, Beatriz Escribano-Bermejo, Ioannis Tzouganakis, Rodrigo P Lacerda, Isabelle Pearce, Georgina V Fricker","doi":"10.1111/vop.13269","DOIUrl":"10.1111/vop.13269","url":null,"abstract":"<p><strong>Objective: </strong>To compare characteristics and treatment outcomes of spontaneous chronic corneal epithelial defects (SCCEDs) in brachycephalic non-Boxers and Boxers. We hypothesized that brachycephalic non-Boxers develop SCCEDs at a younger age and develop complications more frequently than Boxers.</p><p><strong>Animals studied: </strong>Retrospective review of medical records of brachycephalic dogs treated for SCCEDs between January 2018 and December 2022 in multiple ophthalmology referral centers in the UK.</p><p><strong>Procedure: </strong>Data recorded included breed, age, time of onset, treatment prior to referral, treatment at referral, time to heal, need for further procedures, and complications.</p><p><strong>Results: </strong>A total of 464 SCCEDs in 420 dogs were included composed of 173 Boxers with 200 SCCEDs and 247 brachycephalic non-Boxer dogs with 264 SCCEDs. Boxers were significantly older (median 8.2, range 4.5-12.7 years) than brachycephalic non-Boxers (median 7.2, range 1.6-15.9 years) (p < .001). The first treatment (cotton-tipped applicator [CTA] debridement, diamond burr debridement, superficial keratectomy, grid keratotomy, punctate keratotomy, or combinations of these) selected was significantly different between groups (p < .001). Healing, excluding cases addressed by CTA debridement, following the first procedure was significantly more successful in Boxers (p = .049). Excluding cases addressed by CTA debridement, 9.6% of SCCEDs in Boxers (20/200) and 13.4% (32/239) of non-Boxers required more than one procedure. In the non-Boxer group, 9.5% (25/264) developed complications, contrasting with 4% (8/200) in the Boxer group. Non-Boxers were more likely to develop complications after the first treatment (p = .006).</p><p><strong>Conclusion: </strong>Non-Boxer brachycephalic dogs develop SCCEDs younger than Boxers. This study suggests SCCEDs in brachycephalic non-Boxers may be less likely to heal following one mechanical treatment and are more likely to develop complications.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":"501-509"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142005417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary clinical evaluation of the treatment efficacy of a temperature-adjustable cryotherapy device for chronic, recurrent blepharitis in dogs.","authors":"Hyun-Wook Kim, Tae-Won Yoon, Joon-Young Kim","doi":"10.1111/vop.13305","DOIUrl":"10.1111/vop.13305","url":null,"abstract":"<p><strong>Objective: </strong>To preliminarily evaluate the treatment efficacy of a temperature-adjustable cryotherapy device for chronic, recurrent blepharitis in dogs.</p><p><strong>Animals studied: </strong>Five dogs (eight eyes) of different breeds with blepharitis, which was unresponsive to systemic steroids.</p><p><strong>Procedures: </strong>The dogs were subjected to ophthalmic examination, including Schirmer tear test-1 (STT-1), tear film break-up time (TFBUT) test, and slit-lamp biomicroscope examination. Cryotherapy was performed using a device featuring precise low-temperature control. A bandage lens and ointment (2% hypromellose) were applied before cryotherapy to protect the cornea. Carbon dioxide gas at 0°C was sprayed on the lesion for 10 s. In all patients, only topical antibiotics and corticosteroid were applied twice a day; systemic corticosteroids were not used. Pruritus and ocular discharge were scored based on the visual analog scale (VAS) before and 3 weeks after cryotherapy. Qualitative changes in tear film were measured using STT-1 and a TFBUT test. The effects on erythema and swelling were evaluated by ophthalmic examination.</p><p><strong>Results: </strong>Pruritus improvement was confirmed in all eight eyes (five dogs). After cryotherapy, the average VAS score of pruritus significantly decreased from 4.12 ± 2.64 to 0.37 ± 0.51 (p < .001), and the average TFBUT significantly increased from 5.75 ± 4.80 to 13.75 ± 4.43 (p = .010). STT-1 and the VAS score of ocular discharge did not significantly differ. Erythema and swelling were improved in three out of eight eyes.</p><p><strong>Conclusions: </strong>Cryotherapy is effective in treating blepharitis with pruritus and may become a new treatment option in patients in whom systemic corticosteroids are difficult to administer.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":"583-589"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diamond burr debridement of calcific band keratopathy in 24 eyes of 22 horses.","authors":"Sophie Jugant, Alain Regnier, Jean-Yves Douet","doi":"10.1111/vop.13212","DOIUrl":"10.1111/vop.13212","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the safety and effectiveness of diamond burr debridement (DBD) for the treatment of calcific band keratopathy (CBK) in horses.</p><p><strong>Methods: </strong>Medical records from horses with CBK were reviewed over a period of 4 years. Diagnosis of CBK was based on slit-lamp examination findings, and DBD was performed on standing sedated horses. Follow-up was obtained by clinical reevaluation of the treated eyes or a telephone survey with the referring veterinarian.</p><p><strong>Results: </strong>Twenty-two horses of different ages (median: 11 years; range: 5-23) representing 24 CBK-affected eyes (14 left eyes and 10 right eyes) were included in the study. The patients had a medical history of chronic and recurrent uveitis, which required repeated long-term treatments with various combinations of ophthalmic corticosteroids. DBD alone cleared the cornea in 22 affected eyes (92%), but it was combined with blade scraping in two cases with thick calcium plaques. The postoperative medication included a topical antibiotic, atropine drops, and oral flunixin meglumine. Corneal healing was achieved in 21 eyes (87%), 5-21 days (median time 13 days) postoperatively. The three remaining horses were lost to follow-up. Recurrence occurred in 5 of the 21 (24%) reexamined eyes, 3-12.5 weeks (median 8 weeks) postoperatively, and required a second DBD. Four of the 21 eyes ended up being enucleated due to sequelae of uveitis. The main limitations of the study include those inherent to all retrospective clinical data collections.</p><p><strong>Conclusion: </strong>Mechanical DBD can readily and safely clear the cornea in horses with CBK, but recurrence of calcium deposition is possible.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":"549-557"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case report: Medical and surgical management of presumed canine nodular granulomatous episcleritis with progression to posterior nodular scleritis, choroiditis, and optic neuritis.","authors":"Mary Evelyn Pearsall, Amy Knollinger, David Gardiner","doi":"10.1111/vop.13230","DOIUrl":"10.1111/vop.13230","url":null,"abstract":"<p><p>Nodular granulomatous episcleritis (NGE) typically presents as an elevated mass or elevated masses at the limbus and often infiltrates the cornea (episclerokeratitis). In the current report, a granulomatous lesion was observed subretinally in the right eye (OD) of a 5-year-old male castrated American Staffordshire Terrier dog. There was concurrent retinal hemorrhage and detachment OD; the right eye was not visual. Due to poor prognosis for vision and potential for a neoplastic etiology of the mass, staging with higher imaging was recommended but declined by the owner. Therefore, an enucleation was performed. Histopathology of the globe identified a subretinal mass, marked histiocytic and lesser lymphoplasmacytic choroiditis, posterior episcleritis, and optic neuritis with retinal detachment. The subretinal mass was composed of densely packed, large, spindle histiocytes mixed with occasional lymphocytes, plasma cells, and only rare neutrophils. Regions of the mass showed lymphocytes aggregate to form nodules. This histological presentation was a type of proliferative histiocytic disease with similarities to nodular granulomatous episcleritis or granulomatous/necrotizing scleritis. This is a novel presentation of NGE-like progression to subretinal scleral, choroidal, and retinal involvement and provides a new differential possibility for posterior segment masses observed on fundic examination.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":"577-582"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141159014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ophthalmic findings associated with Australian tick paralysis (holocyclotoxicity) in hospitalized domestic dogs and cats.","authors":"B D Reynolds, H G Nagel, E Perry, C J Whittaker, K A Caruso, M J Annear, W M Irving, P M McCarthy, A Dion, J M S Yi, E Hall, J S Smith","doi":"10.1111/vop.13205","DOIUrl":"10.1111/vop.13205","url":null,"abstract":"<p><strong>Objective: </strong>To describe ophthalmic findings in hospitalized canine and feline patients with tick paralysis (TP) and investigate possible predisposing factors.</p><p><strong>Animals studied: </strong>Forty-seven dogs and 28 cats hospitalized with TP assessed with an ophthalmic examination performed by an ABVO resident.</p><p><strong>Methods: </strong>Dogs and cats were hospitalized with TP from October 2021 to January 2022 and had an ophthalmic examination performed by an ABVO resident. Patient signalment data, information regarding tick number and location, hospitalization duration, medications used, and patient paralysis grades were recorded. Statistical analysis was performed to correlate findings.</p><p><strong>Results: </strong>Corneal ulcers developed in up to 34.8% of dogs and up to 42.9% of cats hospitalized with TP. An absent palpebral reflex ipsilaterally increased the odds of a concurrent corneal ulcer being present by 14.7× in dogs and 20.1× in cats (p < .0001). Palpebral reflexes were absent in 38.3% of dogs and 35.7% of cats hospitalized with TP and were correlated with more severe gait paralysis (p = .01) and respiratory paralysis (p = .005) in dogs, and respiratory paralysis in cats (p = .041). STT-1 findings <10 mm/min were present in 27.7% of dogs and 57.1% of cats examined and were associated with increasing gait paralysis (p = .017) and respiratory paralysis (p = .007) in dogs, and increasing gait paralysis in cats (p = .017).</p><p><strong>Conclusions: </strong>Simple corneal ulcers, loss of a complete palpebral reflex, and reduced STT-1 scores frequently occurred in dogs and cats hospitalized for TP. The frequency of these findings increased as the degree of patient paralysis increased.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":"522-529"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the eyeTelemed IOPvet indentation tonometer for use in dogs.","authors":"Lydia E Kapeller, Ava G Cabble, Phillip N Buckman, Christine D Harman, Amanda L Jacobson, Frank R Lawrence, András M Komáromy","doi":"10.1111/vop.13215","DOIUrl":"10.1111/vop.13215","url":null,"abstract":"<p><strong>Objective: </strong>To assess the accuracy of canine intraocular pressure (IOP) estimates from the eyeTelemed IOPvet indentation tonometer.</p><p><strong>Animals studied: </strong>Part 1 included 54 eyes from 28 Beagle dogs-23 ADAMTS10-mutants with open-angle glaucoma and 5 normals. Part 2 involved five normal canine ex vivo globes.</p><p><strong>Procedure: </strong>Part 1 (in vivo) compared IOPvet estimates in normal and glaucomatous dogs to Reichert Tono-Vera® Vet rebound tonometry. The three IOPvet estimates were green (normal; <20 mmHg, according to the manufacturer), yellow (elevated; 20-30 mmHg), and red (high; >30 mmHg). In Part 2 (ex vivo), the pressure inside freshly enucleated normal canine eyes was progressively increased from 5 to 80 mmHg and compared to IOPvet estimates. Descriptive statistics compared IOPvet estimates to rebound tonometry and direct manometry, with the threshold from normal to glaucoma set at 30 mmHg.</p><p><strong>Results: </strong>In Part 1 (in vivo), normal pressures (≤30 mmHg) were mainly identified correctly as green or yellow-110 of 111 estimates, corresponding to a specificity of 99%. Only 16 of 125 affected estimates were correctly displayed in the >30-mmHg range; the remaining 109 showed ≤30 mmHg, corresponding to a sensitivity of 13%. In Part 2 (ex vivo), all normal pressures were correctly estimated with green, but 64 of 88 manometric IOPs >30 mmHg were falsely estimated as 20-30 mmHg.</p><p><strong>Conclusions: </strong>The IOPvet is inaccurate in estimating canine IOP with a low sensitivity at identifying dogs with IOP > 30 mmHg. Canine-specific instrument revision is required to correctly identify elevated (yellow = 20-30 mmHg) and high (red >30 mmHg) IOPs.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":"492-500"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The novel use of a sub-palpebral lavage line as an indwelling stent to manage unilateral nasolacrimal duct obstruction in a cat.","authors":"Anna Catherine Bowden, Rachel A Allbaugh","doi":"10.1111/vop.13297","DOIUrl":"https://doi.org/10.1111/vop.13297","url":null,"abstract":"<p><strong>Objective: </strong>To describe a novel surgical approach to conjunctivorhinostomy for feline nasolacrimal duct obstruction.</p><p><strong>Animal studied: </strong>A client-owned 4-year-old female spayed domestic shorthair cat presented with chronic unilateral nasolacrimal duct obstruction of the right eye.</p><p><strong>Methods: </strong>Following obstruction confirmation via complete ophthalmic examination and dacryocystorhinography, conjunctivorhinostomy was performed under general anesthesia, and an indwelling sub-palpebral lavage (SPL) line was placed to facilitate the formation of a new pathway for tear drainage. Specifically, the SPL footplate was sutured to the ventral conjunctival fornix with 5-0 nylon and 6-0 vicryl while the tubing was passed through the new pathway and the distal end of the tubing trimmed and tucked into a perinasal subcutaneous pocket. Two skin sutures closed the perinasal pocket and were removed 2 weeks postoperatively.</p><p><strong>Results: </strong>No immediate postoperative complications occurred. At 6 weeks and 6 months postoperatively, the distal SPL tubing was dislodged and visible out of the right nostril. This was uneventfully trimmed shorter, and no further complications occurred. The SPL component was easily removed 11 months postoperatively without complication. The patient's clinical signs dramatically improved; she was comfortable over 2 years after surgery.</p><p><strong>Conclusion: </strong>To the authors' knowledge, this is the first report of using an SPL as an indwelling catheter for nasolacrimal duct surgery via conjunctivorhinostomy in a cat. This technique eliminated external stent sites for the patient, which was associated with a short use of an e-collar, and utilized materials readily available in a specialty hospital.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interexaminer agreement of gonioscopy examinations in dogs using the European College of Veterinary Ophthalmologists Hereditary Eye Diseases grading scheme.","authors":"Niklas Holz, Malwina Ewa Kowalska, Simon Anton Pot, Antonella Rampazzo","doi":"10.1111/vop.13292","DOIUrl":"https://doi.org/10.1111/vop.13292","url":null,"abstract":"<p><strong>Objective: </strong>Prospective observational study with preregistered study protocol to assess interexaminer agreement using the 2022 European College of Veterinary Ophthalmologists Hereditary Eye Disease (ECVO-HED) gonioscopy grading scheme.</p><p><strong>Animals studied: </strong>Sixty client-owned dogs presented for gonioscopy as part of the required certification process prior to breeding.</p><p><strong>Procedure: </strong>Two ECVO diplomates with comparable clinical experience performed gonioscopy with a Koeppe gonioscopy lens and slit-lamp biomicroscope at 10x magnification on all eyes in a randomized fashion.</p><p><strong>Results: </strong>One hundred and eighteen of 120 eyes (60 dogs) were included. In 110/118 eyes (93%), both examiners provided the same breeding recommendation (yes/no). This translated into an agreement on the final breeding recommendation in 58/60 dogs (97%). Examiners disagreed on pectinate ligament abnormality (PLA) grading in 19/118 eyes and iridocorneal angle width (ICAW) grading in 9/118 eyes. PLA grading disagreement was mostly observed between neighboring grades. Disagreement between PLA and ICAW grading sometimes occurred within the same eye accounting for an observed disagreement in iridocorneal angle abnormality (ICAA) grading in 19/118 eyes in total. Cohen's kappa was Κ = 0.62 (95% TI 0.34-0.89), whereas maximum kappa was maxΚ = 0.82 (95% TI 0.59-1). Mixed model analysis suggested no significant examiner influence on breeding recommendations (OR 0.17; 95% CI 0.02-1.12, p = .09).</p><p><strong>Conclusions: </strong>Although examiners disagreed on ICAA grading in 19/118 eyes, this resulted in a different breeding recommendation (yes/no) in eight eyes and in two dogs only. Therefore, the use of the 2022 ECVO-HED gonioscopy grading scheme seems to result in examiners providing the same breeding recommendations in most cases.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presumed calcific band keratopathy in a 10-month-old domestic shorthair cat.","authors":"Marta Ferruz-Fernandez, Ana Ripolles-Garcia, Miriam Caro-Suarez, Almudena Latre-Moreno, Laura Jimenez-Ramos, Maria Paloma Rodriguez-Gavilan, Carolina Naranjo, Fernando Laguna, Manuel Villagrasa","doi":"10.1111/vop.13295","DOIUrl":"https://doi.org/10.1111/vop.13295","url":null,"abstract":"<p><strong>Objective: </strong>To describe a case of presumed band keratopathy in a young cat with no known history of corneal disease who was not receiving topical phosphate-based steroids.</p><p><strong>Animal studied: </strong>A 10-month-old domestic shorthair cat was evaluated for severe ocular discomfort that did not improve despite the initiation of topical antibiotic treatment.</p><p><strong>Methods: </strong>A complete ophthalmic examination and ancillary tests such as biomicroscopy and corneal cytology were performed at the time of presentation. A superficial lamellar keratectomy was excised, and the corneal button was sent for histopathology. In addition to routine hematoxylin-eosin staining, sections were stained with Gram, PAS, von Kossa, and trichrome stains.</p><p><strong>Results: </strong>Biomicroscopic examination revealed an extensive corneal epithelial ulcer with a hard white plaque in the central area of the right cornea with associated superficial neovascularization. Ultrasound biomicroscopy showed a 0.63 mm, slightly hyperechoic band in the anterior corneal stroma. Corneal cytology evidenced numerous neutrophils with toxic changes and few macrophages. Histopathologic analysis excluded corneal sequestration as a differential diagnosis and confirmed superficial corneal mineralization. At the last follow-up, 16 months after surgery, corneal transparency had returned, and visual acuity was good with some corneal conjunctivalization.</p><p><strong>Conclusions: </strong>In this case report, the presumptive diagnosis is calcific band keratopathy. To the best of the author's knowledge, this is the first presumed case in a cat not receiving topical phosphate-based steroids and without previous corneal damage. Lamellar keratectomy represents a curative approach that successfully resolved the clinical signs, with no reported recurrence.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the locus for ocular melanosis in Cairn Terriers.","authors":"Paige A Winkler, Ethan M Dawson-Baglien, Madeline C Coffey, Patrick J Venta, Kari J Ekenstedt, Simon M Petersen-Jones","doi":"10.1111/vop.13291","DOIUrl":"https://doi.org/10.1111/vop.13291","url":null,"abstract":"<p><strong>Objective: </strong>To map the disease locus for familial ocular melanosis (OM) in the Cairn Terrier.</p><p><strong>Animals studied: </strong>Cairn Terriers with OM and normal control dogs.</p><p><strong>Procedure: </strong>A genome-wide association study (GWAS) was performed using 63 OM-affected and 31 control Cairn Terriers, followed by haplotype analysis. A significantly associated single-nucleotide polymorphism was genotyped in a larger group of OM-affected and control Cairn Terriers. The coding and splice-site regions of genes mapping within the confidence interval were sequenced.</p><p><strong>Results: </strong>A ~9.2 Mb region of chromosome 11 was significantly associated with OM. Haplotype analysis narrowed the region to 1.49 Mb. Genotyping of a SNP within the region showed 86% of OM-affected dogs were homozygous or heterozygous for the risk allele, whereas 78% of unaffected dogs were homozygous for the nonrisk allele. Sequencing of the coding regions and splice sites of four genes (c9orf72, IFNK, the 5' end of MOB3B, and the 3' end of LINGO2) and of a microRNA (MIR876) did not detect any genetic variants unique to OM-affected dogs.</p><p><strong>Conclusion: </strong>OM in Cairn Terriers maps to a 1.49 Mb region of chromosome 11. This accounts for 86% of OM cases in our DNA bank. A second locus may account for the OM phenotype in the remaining 14% of cases. Sequencing of coding regions and splice sites of positional candidate genes and a microRNA did not reveal any genetic variants unique to affected dogs. Further studies are required to elucidate the DNA variant causal for OM in Cairn Terriers and to understand the disease mechanism.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}