Virologica Sinica最新文献_第4页

Clinical outcomes after HBsAg clearance in chronic hepatitis B patients treated with Peg-IFN α: A study with an 11- to 173-month follow-up Peg-IFN α治疗慢性乙型肝炎患者HBsAg清除后的临床结果：一项随访11至173个月的研究

IF 4 3区医学

Virologica Sinica Pub Date : 2025-08-01 DOI: 10.1016/j.virs.2025.06.008

Wen Deng , Hongxiao Hao , Ziyu Zhang , Xinxin Li , Weihua Cao , Yaqin Zhang , Shiyu Wang , Zixuan Gao , Linmei Yao , Shuojie Wang , Xin Wei , Wei Yi , Linqing Zhao , Yao Xie , Minghui Li

{"title":"Clinical outcomes after HBsAg clearance in chronic hepatitis B patients treated with Peg-IFN α: A study with an 11- to 173-month follow-up","authors":"Wen Deng , Hongxiao Hao , Ziyu Zhang , Xinxin Li , Weihua Cao , Yaqin Zhang , Shiyu Wang , Zixuan Gao , Linmei Yao , Shuojie Wang , Xin Wei , Wei Yi , Linqing Zhao , Yao Xie , Minghui Li","doi":"10.1016/j.virs.2025.06.008","DOIUrl":"10.1016/j.virs.2025.06.008","url":null,"abstract":"<div><div>To investigate the risk and influencing factors of long-term liver adverse events in chronic hepatitis B patients achieving hepatitis B surface antigen (HBsAg) clearance after pegylated interferon α (Peg-IFN α) treatment, a retrospective analysis was conducted on 456 patients at Beijing Ditan Hospital from 2008 to 2023 who achieved HBsAg clearance and discontinued Peg-IFN α treatment. The baseline was defined as the time of HBsAg clearance and treatment cessation. The endpoint was the first occurrence of liver adverse events (hepatocellular carcinoma or ascites) or last follow-up. Subsequently, we evaluated the incidence and risk factors of liver adverse events, along with changes in liver fibrosis, cirrhosis, and liver function indicators. During a median follow-up of 70 months, the incidence of liver adverse events was 2.30%, hepatocellular carcinoma 1.76%, and ascites 0.55%. Older age and cirrhosis were significant risk factors (HR 1.075 and 41.393, both <em>P</em> < 0.01). The APRI score significantly improved at follow-up compared to baseline (0.53 vs. 0.25, <em>P</em> < 0.001), and cirrhosis prevalence decreased from 5.70% to 0.88% (<em>P</em> < 0.001). In conclusion, patients who achieved HBsAg clearance and discontinued Peg-IFN α treatment have a low risk of liver adverse events, while advanced age and cirrhosis remain major risk factors.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 4","pages":"Pages 579-586"},"PeriodicalIF":4.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-circulation of all four DENV serotypes during 2016 outbreak in Sinaloa, Mexico: First report of DENV-4 in patients 墨西哥锡那罗亚州2016年疫情期间登革热病毒所有四种血清型的共循环：首次报告DENV-4患者。

IF 4 3区医学

Virologica Sinica Pub Date : 2025-08-01 DOI: 10.1016/j.virs.2025.07.010

Annete Itzel Apodaca-Medina , José Israel Torres-Avendaño , Hipólito Castillo-Ureta , Edith Hilario Torres-Montoya , Sergio Alonso Durán-Pérez , Lorenzo Ulises Osuna-Martínez , María Elena Báez-Flores , José Guadalupe Rendón-Maldonado

引用次数: 0

Rapid and accurate detection of infectious SARS-CoV-2 by viral receptor capture combined with loop-mediated isothermal amplification 病毒受体捕获结合环介导等温扩增快速准确检测传染性SARS-CoV-2

IF 4 3区医学

Virologica Sinica Pub Date : 2025-08-01 DOI: 10.1016/j.virs.2025.06.006

Zixiao Yang , Xinrong Zhou , Xikui Sun , Liu Cao , Tiefeng Xu , Kun Li , Hongchao Liu , Yanxi Ji , Lihong Liu , Konstantin I. Ivanov , Zhonghan Yang , Deyin Guo , Chun-Mei Li

{"title":"Rapid and accurate detection of infectious SARS-CoV-2 by viral receptor capture combined with loop-mediated isothermal amplification","authors":"Zixiao Yang , Xinrong Zhou , Xikui Sun , Liu Cao , Tiefeng Xu , Kun Li , Hongchao Liu , Yanxi Ji , Lihong Liu , Konstantin I. Ivanov , Zhonghan Yang , Deyin Guo , Chun-Mei Li","doi":"10.1016/j.virs.2025.06.006","DOIUrl":"10.1016/j.virs.2025.06.006","url":null,"abstract":"<div><div>Rapid and accurate detection of infectious virus particles, not just viral nucleic acid, is essential to avoid unnecessary quarantine and effectively control the spread of viral diseases such as coronavirus disease 2019 (COVID-19), severe acute respiratory syndrome (SARS), and Middle East respiratory syndrome (MERS). Real-time quantitative polymerase chain reaction (RT-qPCR) was the most widely used detection technique during the COVID-19 outbreak. However, it cannot discriminate between intact infectious viruses and surface-distorted, non-infectious virus particles or naked viral RNA. In this study, we present a strategy for the specific detection of infectious coronaviruses by combining viral receptor capture and reverse transcription loop-mediated isothermal amplification (RT-LAMP). We successfully applied this strategy to detect infectious virus particles of the SARS-CoV-2 surrogate virus and the human coronavirus NL63 (HCoV-NL63). Virus particles were first captured on ELISA plates coated with the recombinant human angiotensin-converting enzyme 2 (hACE2) receptor. Viral RNA was then extracted from the particles and detected by RT-LAMP using virus-specific primers. In our experimental setting, the proposed method had a minimum detection limit (LOD) of 90 PFU/mL, sensitivity of 96.2%, and specificity of 100%. Our study provides a proof-of-concept that viral receptor capture combined with RT-LAMP can differentiate infectious coronaviruses from non-infectious virions or naked viral RNA. This paves the way for this virus detection strategy to become a mainstream tool for the management, prevention and control of epidemic coronavirus diseases.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 4","pages":"Pages 613-623"},"PeriodicalIF":4.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ARRDC3 promotes lysosome-mediated YAP degradation to inhibit enterovirus replication ARRDC3促进溶酶体介导的YAP降解以抑制肠道病毒复制。

IF 4 3区医学

Virologica Sinica Pub Date : 2025-08-01 DOI: 10.1016/j.virs.2025.07.009

Xia Huang , Siyuan Wang , Yan Huang , Yue Wang , Guangchao Zang , Yan Liang , Juntong Liu , Xinyue Han , Jingjing Liao , Tingting Chen , Nan Lu , Guangyuan Zhang

{"title":"ARRDC3 promotes lysosome-mediated YAP degradation to inhibit enterovirus replication","authors":"Xia Huang , Siyuan Wang , Yan Huang , Yue Wang , Guangchao Zang , Yan Liang , Juntong Liu , Xinyue Han , Jingjing Liao , Tingting Chen , Nan Lu , Guangyuan Zhang","doi":"10.1016/j.virs.2025.07.009","DOIUrl":"10.1016/j.virs.2025.07.009","url":null,"abstract":"<div><div>Enterovirus D68 (EV-D68) and enterovirus A71 (EV-A71) are two major types of enteroviruses that pose emerging challenges to public health and have the potential to cause outbreaks, yet their pathogenic mechanisms remain largely unexplored. Arrestin domain containing 3 (ARRDC3) is a vital regulator of glucose metabolism, cancer development, and inflammation. Whether ARRDC3 contributes to innate antiviral immunity is undefined. Here, we found that enterovirus infection induces ARRDC3 expression at both the mRNA and protein levels, thereby inhibiting enterovirus replication. Moreover, we demonstrate that the expression of Yes-associated protein (YAP), a key effector of the Hippo pathway, is severely downregulated by ARRDC3 via lysosomal pathway. YAP facilitates enterovirus replication by suppressing the interferon pathway during the later stage of enterovirus infection, independent of its transcriptional activity. Finally, the ARRDC3-YAP pathway exhibits a broad-spectrum antiviral effect in various viral infections, including those caused by human parainfluenza virus type 3 (HPIV3) and vesicular stomatitis virus (VSV). Collectively, our results identify the critical role of ARRDC3 and its negative regulatory effect on YAP in the innate antiviral response, suggesting a novel therapeutic strategy against virus infection.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 4","pages":"Pages 658-668"},"PeriodicalIF":4.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin inhibits herpes simplex virus 1 replication in corneal epithelium and suppresses keratitis progression 槲皮素抑制单纯疱疹病毒1型在角膜上皮中的复制并抑制角膜炎进展。

IF 4 3区医学

Virologica Sinica Pub Date : 2025-08-01 DOI: 10.1016/j.virs.2025.07.007

Yubin Yu , Sihao Liu , Qinghua Liu , Xiuping Liu , Kaili Wu

引用次数: 0

The slow progression of Japanese encephalitis in aged mice is likely associated to B cell recruitment in the brain 老年小鼠乙型脑炎的缓慢进展可能与脑内B细胞募集有关。

IF 4 3区医学

Virologica Sinica Pub Date : 2025-08-01 DOI: 10.1016/j.virs.2025.05.013

Zheng-Ran Song , Yi-Lin Yang , Yang Zhou , Li-Bo Liu , Fei-Yang Xue , Lin-Shen-Yang Liu , Na Gao , Dong-Ying Fan , Yi-Song Wang , Jing An , Pei-Gang Wang

{"title":"The slow progression of Japanese encephalitis in aged mice is likely associated to B cell recruitment in the brain","authors":"Zheng-Ran Song , Yi-Lin Yang , Yang Zhou , Li-Bo Liu , Fei-Yang Xue , Lin-Shen-Yang Liu , Na Gao , Dong-Ying Fan , Yi-Song Wang , Jing An , Pei-Gang Wang","doi":"10.1016/j.virs.2025.05.013","DOIUrl":"10.1016/j.virs.2025.05.013","url":null,"abstract":"<div><div>The Japanese encephalitis virus (JEV) causes Japanese encephalitis (JE), a severe disease that primarily affects children and induces significant central nervous system complications. With the widespread adoption of vaccination in children, the incidence among older individuals has increased substantially. Despite this epidemiological shift, research on JEV infection in the elderly remains limited. We established JEV infection models using both aged and young mice to explore age-related differences in pathology and underlying mechanisms. Brain tissue samples were analyzed for pathological changes and viral tropism in major cell types. To further characterize immune response variations, we conducted transcriptomic sequencing on the brain tissues following JEV infection. Aged mice exhibited lower mortality, delayed disease progression, and milder brain pathology compared to young mice after JEV infection. Viral titers and infection rates of major brain cell types were similar in both groups. Transcriptomic analysis revealed diminished immune activation and weaker inflammatory responses in aged mice. Additionally, microglial activation and CD8<sup>+</sup> T cell function were significantly reduced. Interestingly, JEV infection induced the selective recruitment of B cells in the brains of aged mice. These B cells may modulate the effects of CD8<sup>+</sup> T cells in the disease process. Compared to young mice, aged mice showed enhanced resistance to JEV progression and reduced brain pathology. This resistance was associated with a weakened immune response in the aged brain, rather than differences in viral infection. The specific recruitment of B cells in the brains of aged mice may play a crucial role in limiting disease progression.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 4","pages":"Pages 546-559"},"PeriodicalIF":4.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of PEDV inhibitors targeting 3CL protease 靶向3CL蛋白酶的PEDV抑制剂的鉴定。

IF 4 3区医学

Virologica Sinica Pub Date : 2025-08-01 DOI: 10.1016/j.virs.2025.06.002

Ang Tian , Shutong Shi , Siying Zou , Shuaiyin Guan , Hao Wu , Zhen Li , Huanchun Chen , Yunfeng Song

{"title":"Identification of PEDV inhibitors targeting 3CL protease","authors":"Ang Tian , Shutong Shi , Siying Zou , Shuaiyin Guan , Hao Wu , Zhen Li , Huanchun Chen , Yunfeng Song","doi":"10.1016/j.virs.2025.06.002","DOIUrl":"10.1016/j.virs.2025.06.002","url":null,"abstract":"<div><div>Porcine epidemic diarrhea (PED), caused by porcine epidemic diarrhea virus (PEDV), is a highly contagious gastrointestinal disease characterized by vomiting, diarrhea, and dehydration, with mortality rates approaching 100% among suckling piglets. The PEDV 3C-like protease (3CLpro) is essential for viral replication and regarded as a critical target for antiviral inhibitor development. In this study, we aimed to identify small-molecule inhibitors of PEDV by targeting 3CLpro. Virtual screening of 1.6 million compounds from the ChemDiv library identified four potential candidates. Molecular dynamics simulations, specifically analyzing RMSD, RMSF, and Rg, demonstrated increased structural stability of the compound-protease complexes compared to the monomeric enzyme. All compounds had low cytotoxicity in Vero cells (CC<sub>50</sub> > 200 μM). Fluorescence resonance energy transfer-based assays demonstrated dose-dependent inhibitory activity of the compounds against 3CLpro. Among the candidates, compound F366-0161 exhibited the weakest inhibition, with an IC<sub>50</sub> value of 151.5 μM. Two analogues, 3238-0395 (IC<sub>50</sub> of 121.4 μM) and L878-0493 (IC<sub>50</sub> of 123.6 μM), exhibited moderately enhanced activity. Y041-1672 was identified as the most effective inhibitor, with an IC<sub>50</sub> of 86.48 μM. In viral replication inhibition assays, Y041-1672 reduced PEDV replication, with an EC<sub>50</sub> of 17.97 μM and a selectivity index (SI) of 15.5 (CC<sub>50</sub>/EC<sub>50</sub>). These results were validated by RT-qPCR, plaque assays, immunofluorescence, and Western blot analyses. <em>In vitro</em> validation confirmed Y041-1672 as the optimal antiviral candidate, and time-of-addition experiments indicated that inhibition primarily occurred during viral replication. This study identifies scaffold molecules for PEDV antiviral drug development, providing strategic insights for PED treatment.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 4","pages":"Pages 624-635"},"PeriodicalIF":4.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Black swans as sentinel species for the emergence of clade 2.3.4.4b highly pathogenic avian influenza (H5N1) in Shanghai, China, 2024. 2024年中国上海2.3.4.4b支高致病性禽流感（H5N1）出现的黑天鹅作为前哨物种

IF 4 3区医学

Virologica Sinica Pub Date : 2025-07-19 DOI: 10.1016/j.virs.2025.07.008

Yuting Xu, Shihai Wu, Lei Ji, Jie Hu, Jin Wang, Ke Li, Yue Yuan, Gaojian Li, Guangjian Zhu, Panyu Hua, Qiuhong Miao, Hongxuan He, Guimei He

引用次数: 0

SNX10 enhances HCoV-OC43 infection by facilitating viral entry and inhibiting virus-triggered autophagy. SNX10通过促进病毒进入和抑制病毒引发的自噬来增强HCoV-OC43感染。

IF 4 3区医学

Virologica Sinica Pub Date : 2025-07-09 DOI: 10.1016/j.virs.2025.07.005

Haobin Li, Huiyi Guo, Binhao Rong, Haowei Li, Wenjiao Wu, Chan Yang, Shuwen Liu

{"title":"SNX10 enhances HCoV-OC43 infection by facilitating viral entry and inhibiting virus-triggered autophagy.","authors":"Haobin Li, Huiyi Guo, Binhao Rong, Haowei Li, Wenjiao Wu, Chan Yang, Shuwen Liu","doi":"10.1016/j.virs.2025.07.005","DOIUrl":"10.1016/j.virs.2025.07.005","url":null,"abstract":"<p><p>The ongoing coronavirus epidemic, including the novel coronavirus (SARS-CoV-2), continues to pose a significant threat to global public health. Host targets address multiple stages of the viral life cycle and provide diverse opportunities for therapeutic interventions. This study identified sorting nexin 10 (SNX10) as a facilitator of replication of human coronavirus OC43 (HCoV-OC43), underscoring its potential as a novel antiviral target. The knockout of SNX10 significantly suppressed HCoV-OC43 replication both in vivo and in vitro. Immunoprecipitation-mass spectrometry (‌IP-MS) analysis identified the adaptor protein complex 2 subunit μ1 (AP2M1) as a direct interactor of SNX10. Specifically, SNX10 facilitates phosphorylation of the AP2M1, thereby enhancing clathrin-mediated viral endocytosis. Furthermore, subsequent binding and internalization assays revealed that SNX10 knockout significantly inhibits viral entry into host cells. Conversely, the reconstitution of SNX10 fully restored viral entry, thereby confirming the critical and indispensable role of SNX10 in pathogen internalization. Simultaneously, SNX10 was identified as a key factor that promotes endosomal acidification by modulating pH levels, which in turn facilitated the release of the viral genome. Notably, the ablation of SNX10 was found to trigger autophagy activation during infection, thereby maintaining intracellular homeostasis. Additionally, it exerted autonomous antiviral effects through lysosomal degradation pathways. Collectively, these findings demonstrate SNX10 serves as a pivotal regulator of the viral life cycle and underscore its therapeutic potential as a multi-faceted antiviral candidate target capable of simultaneously inhibiting viral internalization, viral genomic release, and host-pathogen equilibrium.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144620767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential susceptibility of immunodeficient mice to MPXV infection and the impact of various inoculation routes 免疫缺陷小鼠对MPXV感染的差异易感性及不同接种途径的影响。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-01 DOI: 10.1016/j.virs.2025.04.001

Xiaohan Wang , Shaowen Shi , Xiaoxuan Nie , Yongyang Sun , Jinglei Hu , Manlin He , Wenhao Ren , Yuxing Wang , Zhendong Guo , Gonghe Li , Changbo Ou , Xiao Li , Zongzheng Zhao

{"title":"Differential susceptibility of immunodeficient mice to MPXV infection and the impact of various inoculation routes","authors":"Xiaohan Wang , Shaowen Shi , Xiaoxuan Nie , Yongyang Sun , Jinglei Hu , Manlin He , Wenhao Ren , Yuxing Wang , Zhendong Guo , Gonghe Li , Changbo Ou , Xiao Li , Zongzheng Zhao","doi":"10.1016/j.virs.2025.04.001","DOIUrl":"10.1016/j.virs.2025.04.001","url":null,"abstract":"<div><div>Monkeypox virus (MPXV), a member of the <em>Orthopoxvirus</em> genus, caused a large-scale global outbreak in 2022. Developing mouse models for MPXV infection is crucial for advancing research on vaccines and therapeutic interventions. To address this, we conducted a comparative study on the susceptibility of six mouse strains—severe combined immune-deficiency (SCID), nude, genetically diabetic (db/db) and obese (ob/ob), C57BL/6J, and BALB/c—to MPXV infection. Mouse strains were infected with MPXV via intranasal inoculation, and body weight changes and mortality were monitored post-infection. Additionally, the tissue distribution of MPXV and the pathological changes in the lung tissues of the infected mice were evaluated. The results demonstrated that SCID and nude mice exhibited significant weight loss following MPXV infection, with 100 % mortality observed in SCID mice, while no mortality occurred in nude mice. In contrast, the other mouse strains showed no significant weight loss or mortality. Notably, the viral load in the lung tissues of SCID and nude mice was the highest among the tested strains. Furthermore, we investigated the impact of different inoculation routes—intranasal (I.N.), intraperitoneal (I.P.), and intravenous (I.V.)—on the pathogenicity of MPXV in mice. The results revealed that the intravenous route induced more pronounced pathogenic effects compared to the intranasal and intraperitoneal routes. In summary, this study provides valuable insights into the development of MPXV-infected mouse models, offering a foundation for further research on MPXV pathogenesis and therapeutic drug development.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 3","pages":"Pages 333-339"},"PeriodicalIF":5.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0