Virologica Sinica最新文献_第4页

Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies 用于抗病毒药物筛选和生命周期研究的报告毒株HBoV1的研制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.009

Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen

{"title":"Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies","authors":"Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen","doi":"10.1016/j.virs.2025.03.009","DOIUrl":"10.1016/j.virs.2025.03.009","url":null,"abstract":"<div><div>Human bocavirus 1 (HBoV1; family: <em>Parvoviridae</em>) causes a wide spectrum of respiratory diseases in children and gastroenteritis in adults. A lack of sensitive cell lines and efficient animal models hinders research on HBoV, including the development of anti-HBoV drugs or vaccines. Although the construction of a wild-type HBoV1 infectious clone has been reported, generating HBoV1 infectious clone carrying foreign reporter genes with suitable insertion sites in its genome while retaining replicative ability remains challenging. Here, HBoV1 infectious clones harboring the 11-amino-acid HiBiT tag at five distinct insertion sites were constructed and evaluated. Only the recombinant HBoV1 carrying the HiBiT tag in the N-terminus of the NS1 protein (HBoV1-HiBiT<sub>NS1</sub>) displayed comparable characteristics to wild-type HBoV1 as determined via the analysis of viral DNA copy number, NanoLuc activity, viral protein expression, and the formation of replication intermediates. Notably, the replication kinetics of HBoV1-HiBiT<sub>NS1</sub> could be examined by monitoring NanoLuc activity, which was noted to be correlated with the viral DNA level. Additionally, we successfully applied HiBiT-tagged HBoV1 for the evaluation of antiviral drug activity and identified ivermectin (EC50 = 2.27 μM) as a potent anti-HBoV1 replication drug. Overall, our study demonstrated that the HBoV1-HiBiT<sub>NS1</sub> reporter can serve as a convenient platform for screening candidate drugs targeting HBoV1 replication and may also be useful for investigating the life cycle of the virus.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 275-283"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4+ T cell 宿主因子 Naf1 限制了 HIV-1 对髓细胞的感染，并削弱了树突状细胞为 CD4+ T 细胞提供能量的能力。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.007

Chengzuo Xie , Xia Jin , Wan-Wei Li , Jian-Hua Wang

{"title":"Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4+ T cell","authors":"Chengzuo Xie , Xia Jin , Wan-Wei Li , Jian-Hua Wang","doi":"10.1016/j.virs.2025.03.007","DOIUrl":"10.1016/j.virs.2025.03.007","url":null,"abstract":"<div><div>Naf1 (Nef-associated factor 1) is a host protein that interacts with human immunodeficiency virus type 1 (HIV-1) Nef protein. We and others have previously demonstrated that Naf1 restricts HIV-1 infection of T-lymphocytes. Myeloid cells are targets for HIV infection, but Naf1 expression in myeloid cells and whether it also regulates HIV infection in these cells are not yet identified. In this study, we found that Naf1 had a higher expression in CD14<sup>+</sup> monocytes than in monocyte-derived dendritic cells (MDDCs), and its expression in both types of cells could be induced by HIV-1 gp120 glycoproteins or viral particles. Importantly, the expression of Naf1 restricted HIV-1 infection in monocytes and MDDCs. Functional investigation showed that both the constitutive and the induced expression of Naf1 inhibited NF-κB signaling in MDDCs and reduced the basal level or LPS (Lipopolysaccharide)-stimulated production of cytokines. Moreover, Naf1 reduced the expression of ICAM-1 (intercellular cell adhesion molecule-1) on MDDCs and compromised their capacity to prime the activation of resting CD4<sup>+</sup> T cells in co-culture. In light of the essential role of NF-κB signaling for HIV-1 transcription, Naf1-mediated inhibition of NF-κB signaling may hinder a robust viral replication in MDDCs and help maintain viral persistence. Furthermore, virus-induced Naf1 expression in MDDCs may diminish the cross-talk between DC (dendritic cell) and T cells, hence suppressing the activation of antiviral immune responses. Taken together, we identified the new function of Naf1 in myeloid cells. Those findings may facilitate the understanding for the host restriction of HIV-1 infection in myeloid cells.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 217-224"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qingqi Guxue Decoction induces S cell cycle arrest to inhibit replication of severe fever with thrombocytopenia syndrome virus 青芪归脾汤能诱导 S 细胞周期停滞，抑制严重发热伴血小板减少综合征病毒的复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.011

Xixi Shi , Zining Wang , Zixiang Liu , Qinting Lin , Mengqian Huang , Tze Yean Lim , Xiaoyan Li , Tao Wang

{"title":"Qingqi Guxue Decoction induces S cell cycle arrest to inhibit replication of severe fever with thrombocytopenia syndrome virus","authors":"Xixi Shi , Zining Wang , Zixiang Liu , Qinting Lin , Mengqian Huang , Tze Yean Lim , Xiaoyan Li , Tao Wang","doi":"10.1016/j.virs.2025.03.011","DOIUrl":"10.1016/j.virs.2025.03.011","url":null,"abstract":"<div><div>Severe fever with thrombocytopenia syndrome (SFTS) is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus (SFTSV), characterized by high fever and thrombocytopenia. It has been proved that traditional Chinese medicine (TCM) has displayed definite therapeutic effects on viral hemorrhagic fever, indicating its potential to treat SFTS. In this study, SFTS-relative key targets were predicted via gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis. Molecular docking was then used to select stable binders. Molecules matched TCMs were identified, and a new prescription, Qingqi Guxue decoction (QQGX), was formulated to clear heat and nourish blood, with a resulting drug composition network. We explored the optimal drug proportion for QQGX. Through an in-depth study of molecular mechanisms, we found that QQGX induces S phase arrest by promoting the degradation of cyclin A2 (CCNA2) and cyclin-dependent kinase 2 (CDK2), thereby inhibiting SFTSV replication. Finally, we verified the effectiveness and safety of QQGX based on the mouse liver bile duct organoid model infected with SFTSV. In summary, our study prepared a TCM decoction using the method of network pharmacology. This decoction has a significant inhibitory effect on the replication of SFTSV and provides a new treatment strategy for hemorrhagic fever with TCM.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 260-274"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into cross-species infection by coronavirus: Porcine epidemic diarrhea virus infections in the rodent. 冠状病毒跨物种感染的认识：猪流行性腹泻病毒在啮齿动物中的感染。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-03-27 DOI: 10.1016/j.virs.2025.03.012

Jianing Chen, Zemei Wang, Shengyu Lin, Gao Menglin, Yongheng Shao, Shuxian Li, Qingbo Chen, Yaru Cui, Yonghao Hu, Guangliang Liu

{"title":"Insights into cross-species infection by coronavirus: Porcine epidemic diarrhea virus infections in the rodent.","authors":"Jianing Chen, Zemei Wang, Shengyu Lin, Gao Menglin, Yongheng Shao, Shuxian Li, Qingbo Chen, Yaru Cui, Yonghao Hu, Guangliang Liu","doi":"10.1016/j.virs.2025.03.012","DOIUrl":"https://doi.org/10.1016/j.virs.2025.03.012","url":null,"abstract":"<p><p>The cross-species infection of coronaviruses has resulted in several major epidemics since 2003. Therefore, it is of great importance to explore the host ranges of coronaviruses and their features among different hosts. In this study, the porcine epidemic diarrhea virus (PEDV), with swine as the only natural reservoir, was detected in rat fecal samples collected from pig farms. Further animal tests showed PEDV can cause systemic infections in neonate mice and rats. The brain, lung intestine and spleen were all targets for PEDV in rodents in contrast to the intestine being targeted in pigs. Morbidity and mortality vary via different infection routes. PEDV was also detectable in feces after infection, suggesting that the infected rodents were potential infectious sources. Moreover, the cerebric tropism of PEDV was verified in piglets, which had not been identified before. In conclusion, our findings demonstrate that PEDV can cross the species barrier to infect mice and rats through different routes. Although it is highly devastating to piglets, PEDV changes the target organs and turns to be milder when meeting with new hosts. Based on these findings, more attention should be paid to the cross-species infection of PEDV to avoid the emergence of another zoonosis.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and immunoprotection assessment of novel vaccines for avian infectious bronchitis virus. 禽传染性支气管炎病毒新型疫苗的研制及免疫保护效果评价。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-03-25 DOI: 10.1016/j.virs.2025.03.008

Benli Huang, Sheng Chen, Zhanxin Wang, Keyu Feng, Yutao Teng, Ruoying Li, Guanming Shao, Jiaqian Rao, Xinheng Zhang, Qingmei Xie

{"title":"Development and immunoprotection assessment of novel vaccines for avian infectious bronchitis virus.","authors":"Benli Huang, Sheng Chen, Zhanxin Wang, Keyu Feng, Yutao Teng, Ruoying Li, Guanming Shao, Jiaqian Rao, Xinheng Zhang, Qingmei Xie","doi":"10.1016/j.virs.2025.03.008","DOIUrl":"https://doi.org/10.1016/j.virs.2025.03.008","url":null,"abstract":"<p><p>Infectious bronchitis (IB), a highly contagious acute respiratory disease affecting avian species, poses significant challenges to poultry production. The causative agent, Infectious Bronchitis Virus (IBV), exhibits a high mutation rate, leading to limited cross-protection by existing vaccines. This necessitates the development of novel vaccines. This study, based on preliminary investigations conducted by our research team, identified six potential strains (PYG QX1, ZQF QX2, FQH QX3, LYZ QX4, XXX QX5, and CSL strains) for vaccine development. Previous pathogenicity test and serum cross-neutralization experiments conducted in this study have demonstrated that the FQH QX3 strain exhibited the weakest pathogenicity and the broadest spectrum of serum neutralization, while the CSL strain showed the highest pathogenicity and was the most challenging to neutralize, posing the greatest difficulty in prevention and control. Subsequently, we constructed and rescued recombinant vaccine candidates, H120-FQH QX3, and H120-CSL, expressing the S1 and N proteins of the FQH QX3 and CSL strains, respectively. Immunization protection experiments indicated that the H120-CSL recombinant vaccine candidate exhibited the most effective immune protection, making it a promising candidate for further study and evaluation as a recombinant vaccine. The S1 and N genes of the CSL strain demonstrated strong immunogenicity, making them potential candidate antigen genes for future vaccine development.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SARS-CoV-2 3CL protease inhibits pyroptosis through the cleavage of gasdermin D. sars - cov - 23cl蛋白酶通过裂解气皮蛋白D抑制焦亡。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-03-19 DOI: 10.1016/j.virs.2025.03.006

Yecheng Zhang, Xinlei Ji, Dan Huang, Gen Lu, Xinwen Chen

{"title":"The SARS-CoV-2 3CL protease inhibits pyroptosis through the cleavage of gasdermin D.","authors":"Yecheng Zhang, Xinlei Ji, Dan Huang, Gen Lu, Xinwen Chen","doi":"10.1016/j.virs.2025.03.006","DOIUrl":"10.1016/j.virs.2025.03.006","url":null,"abstract":"<p><p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of novel coronavirus disease 2019, can cause acute respiratory symptoms and even death globally. However, the immune escape mechanism and viral pathogenesis remain poorly understood. Here, we report that the SARS-CoV-2 3C-like (3CL) protease specifically cleaves gasdermin D (GSDMD) at Q29 and Q193, producing two N-terminal fragments, GSDMD<sub>1</sub><sub>-</sub><sub>29</sub> and GSDMD<sub>1</sub><sub>-</sub><sub>193</sub>. We also found that SARS-CoV-2 infection induced the cleavage of GSDMD. Then, we demonstrated that the ability to cleave GSDMD was dependent on the protease activity of the 3CL protease. Interestingly, unlike the GSDMD<sub>1-275</sub> fragment cleaved by caspase-1, GSDMD<sub>1</sub><sub>-</sub><sub>29</sub> and GSDMD<sub>1</sub><sub>-</sub><sub>193</sub> did not trigger pyroptosis or inhibit SARS-CoV-2 replication. Additionally, various RNA viral proteases display different preferences for cleaving GSDMD at Q29 and Q193. Our findings reveal a mechanism by which SARS-CoV-2 and other RNA viruses inhibit pyroptosis, highlighting the critical role of the 3CL protease in immune evasion and viral replication.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into recent advancements in human and animal rotavirus vaccines: Exploring new frontiers 人类和动物轮状病毒疫苗的最新进展：探索新领域。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.001

Ahmed H. Ghonaim , Sherin R. Rouby , Wedad M. Nageeb , Ashraf Ahmed Elgendy , Rong Xu , Changsheng Jiang , Noha H. Ghonaim , Qigai He , Wentao Li

引用次数: 0

Distinct tropisms of HCMV and SARS-CoV-2 in lung tissue of a patient with advanced HIV disease 晚期HIV患者肺组织中HCMV和SARS-CoV-2的明显趋向性

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2025.01.001

Jingjing Xu , Bo Yang , Ye Zheng , Yuexiang Yang , Min-Hua Luo , Yun Ling , Xiaohong Fan , Han Cheng

引用次数: 0

A low pathogenic avian influenza A/Mallard/South Korea/KNU2019-34/2019 (H1N1) virus has the potential to increase the mammalian pathogenicity 低致病性禽流感A/野鸭/韩国/KNU2019-34/2019 （H1N1）病毒具有提高哺乳动物致病性的潜力。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.005

Jaemoo Kim , Jungho Kim , Suhyeon Heo , Chang-Hun Yeom , Bao Tuan Duong , Haan Woo Sung , Seon-Ju Yeo , Hyun Park , Haryoung Poo , Jihyun Yang

{"title":"A low pathogenic avian influenza A/Mallard/South Korea/KNU2019-34/2019 (H1N1) virus has the potential to increase the mammalian pathogenicity","authors":"Jaemoo Kim , Jungho Kim , Suhyeon Heo , Chang-Hun Yeom , Bao Tuan Duong , Haan Woo Sung , Seon-Ju Yeo , Hyun Park , Haryoung Poo , Jihyun Yang","doi":"10.1016/j.virs.2024.12.005","DOIUrl":"10.1016/j.virs.2024.12.005","url":null,"abstract":"<div><div>Influenza, a highly contagious respiratory infectious disease caused by an influenza virus, is a threat to public health worldwide. Avian influenza viruses (AIVs) have the potential to cause the next pandemic by crossing the species barrier through mutation of viral genome. Here, we investigated the pathogenicity of AIVs obtained from South Korea and Mongolia during 2018–2019 by measuring viral titers in the lungs and extrapulmonary organs of mouse models. In addition, we assessed the pathogenicity of AIVs in ferret models. Moreover, we compared the ability of viruses to replicate in mammalian cells, as well as the receptor-binding preferences of AIV isolates. Genetic analyses were finally performed to identify the genetic relationships and amino acid substitutions between viral proteins during mammalian adaptation. Of the 24 AIV isolates tested, A/Mallard/South Korea/KNU2019-34/2019 (KNU19-34; H1N1) caused severe bodyweight loss and high mortality in mice. The virus replicated in the lungs, kidneys, and heart. Importantly, KNU19-34-infected ferrets showed high viral loads in both nasal washes and lungs. KNU19-34 replicated rapidly in A549 and bound preferentially to human like α2,6-linked sialic acids rather than to avian-like α2,3-linked sialic acids, similar to the pandemic A/California/04/2009 (H1N1) strain. Gene segments of KNU19-34 were distributed in Egypt and Asia lineages from 2015 to 2018, and the virus had several amino acid substitutions compared to H1N1 AIV isolates that were non-pathogenic in mice. Collectively, the data suggest that KNU19-34 has zoonotic potential and the possibility of new mutations responsible for mammalian adaptation.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 24-34"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential mechanisms and material basis of Fuzheng Jiedu decoction broad-spectrum inhibiting coronaviruses 扶正解毒汤广谱抑制冠状病毒的潜在机制和物质基础。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.007

Ke Liu , Bixia Hong , Shi-Ting He , Siying Du , Jiayi Ke , Lili Tian , Tao Tao , Yihan Zhang , Kelin Li , Han Chang , Mengzhe Li , Xiaoping An , Lihua Song , Zhongde Zhang , Liang Liu , Hudan Pan , Huahao Fan , Yigang Tong

{"title":"The potential mechanisms and material basis of Fuzheng Jiedu decoction broad-spectrum inhibiting coronaviruses","authors":"Ke Liu , Bixia Hong , Shi-Ting He , Siying Du , Jiayi Ke , Lili Tian , Tao Tao , Yihan Zhang , Kelin Li , Han Chang , Mengzhe Li , Xiaoping An , Lihua Song , Zhongde Zhang , Liang Liu , Hudan Pan , Huahao Fan , Yigang Tong","doi":"10.1016/j.virs.2024.12.007","DOIUrl":"10.1016/j.virs.2024.12.007","url":null,"abstract":"<div><div>Traditional Chinese medicine has unique advantages in preventing and treating COVID-19, and Fuzheng Jiedu decoction (FZJDD) was reported to be effective against COVID-19 in clinical trials. To investigate the potential mechanisms and material basis of FZJDD against SARS-CoV-2, we performed SARS-CoV-2 target protein inhibition analyses and a metabolite full spectrum analysis of FZJDD. Interestingly, FZJDD was found to block the binding of SARS-CoV-2 Spike protein with the receptor ACE2 and inhibit the activity of SARS-CoV-2 3CLpro. Moreover, FZJDD can regulate the TNF and the MAPK signaling pathway to inhibit the inflammatory response and alleviate the “cytokine storm”. A total of 298 compounds were identified in FZJDD, among them, caffeic acid and octyl gallate were found to be the potential therapeutic agents of FZJDD. Importantly, FZJDD can broadly inhibit coronavirus infection, including SADS-CoV and porcine epidemic diarrhea virus (PEDV) live viruses, SARS-CoV, MERS-CoV, and SARS-CoV-2 mutant pseudotyped viruses, which might be ascribed to the broad-spectrum anti-coronavirus activity of caffeic acid and octyl gallate. In conclusion, this study reveals the mechanisms and material basis of FZJDD against SARS-CoV-2 and identifies the broad-spectrum anti-coronavirus activity of FZJDD for the first time. Our data provide empirical evidence for the development and application of FZJDD.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 125-136"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0