Virologica Sinica最新文献_第4页

Identification and genetic analysis of new ephemeroviruses in wild boars in China 中国野猪新蜉蝣病毒的鉴定与遗传分析。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.02.002

Zhongzhong Tu , Tong Wang , Yu Xu , Heting Sun , Peng Peng , Siyuan Qin , Changchun Tu

{"title":"Identification and genetic analysis of new ephemeroviruses in wild boars in China","authors":"Zhongzhong Tu , Tong Wang , Yu Xu , Heting Sun , Peng Peng , Siyuan Qin , Changchun Tu","doi":"10.1016/j.virs.2025.02.002","DOIUrl":"10.1016/j.virs.2025.02.002","url":null,"abstract":"<div><div>Ephemeroviruses (EVs) are arthropod-borne rhabdoviruses and were isolated exclusively from cattle and haematophagous arthropods until two new ephemeroviruses were first identified from domestic pigs most recently. Here we report the identification of newer EVs in wild boar by meta-transcriptomic (MTT) sequencing. Further screening by specific RT-nPCR of tissue samples of 459 free-ranging wild boars collected between 2018 and 2023 from 26 provinces across China confirmed five positive wild boars in four provinces. Interestingly, two ticks especially collected from two positive wild boars were also EV positive. Finally, four complete genome sequences of wild boar ephemeroviruses (WbEVs) were obtained with two strains belonging to a new EV species, and the rest two falling into porcine ephemerovirus 2 (PoEV2) species identified from domestic pigs. Our study has further extended EV host range and demonstrated natural circulations of divergent EVs in wild boars, in which ticks may play roles. Biological implications of EV infection in wild boars should be interesting topics for future investigations.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 186-191"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Issue Cover 覆盖问题

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/S1995-820X(25)00047-1

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Artemisia annua L. leaf extracts suppress influenza virus infection by targeting the viral nucleoprotein and blocking mitochondria-mediated apoptosis 黄花蒿叶提取物通过靶向病毒核蛋白和阻断线粒体介导的细胞凋亡抑制流感病毒感染。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.001

Xiwen Zhao , Xuan Dai , Fuyi Wang , Chenyang Li , Xun Song , Yingying Han , Chaowei Zhang , Lu Wang , Zhendan He , Rongping Zhang , Liang Ye

{"title":"Artemisia annua L. leaf extracts suppress influenza virus infection by targeting the viral nucleoprotein and blocking mitochondria-mediated apoptosis","authors":"Xiwen Zhao , Xuan Dai , Fuyi Wang , Chenyang Li , Xun Song , Yingying Han , Chaowei Zhang , Lu Wang , Zhendan He , Rongping Zhang , Liang Ye","doi":"10.1016/j.virs.2025.03.001","DOIUrl":"10.1016/j.virs.2025.03.001","url":null,"abstract":"<div><div><em>Artemisia annua</em> L. is a medicinal herb with multiple therapeutic applications, whereas its anti-influenza A virus (IAV) efficiency and mechanism of action are still unclear. Here, we investigated the inhibition activity and mechanism of <em>A. annua</em> leaf methanol extracts (AALME) against IAV <em>in vitro</em> and <em>in vivo</em>. Our results revealed that AALME exhibits potent anti-IAV activity by interacting with IAV particles. Mechanistically, AALME directly targets the IAV nucleoprotein (NP) protein and abolishes the nuclear import of IAV NP. AALME profoundly suppresses IAV-induced mitochondrial apoptosis via suppressing ROS-mediated AIF-dependent pathways. More importantly, we found that AALME plays a crucial role in protecting mice from IAV infection and mitigating IAV pathogenicity. This current work provides mechanistic insight into the mechanism by which AALME controls IAV infection <em>in vitro</em> and <em>in vivo</em>, potentially contributing to the development of antiviral treatments for IAV infection.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 247-259"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Characterization of novel highly pathogenic avian influenza A(H5N6) clade 2.3.4.4b virus in wild birds, East China, 2024 中国东部地区野生鸟类新型高致病性禽流感A（H5N6）分支2.3.4.4b病毒特征分析

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.01.002

Renjie Sun , Xiaoxiao Feng , Jing Huang , Fangyu Zheng , Ronghui Xie , Chuanliang Zhang , Hongli Zhang , Yin Xue , Aijun Liu , Xiaobing Huang , Lin Yuan , Lingyan Zhao

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Identification of an immunodominant neutralizing epitope of porcine astrovirus type 5 capsid protein 猪星状病毒5型衣壳蛋白免疫显性中和表位的鉴定。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.003

Yixin Hu , Zehui Li , Chenlin Hao , Hao Lu , Yunfei Xing , Kexin Liu , Xiaohui Jin , Zhanyong Wei

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Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies 用于抗病毒药物筛选和生命周期研究的报告毒株HBoV1的研制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.009

Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen

{"title":"Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies","authors":"Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen","doi":"10.1016/j.virs.2025.03.009","DOIUrl":"10.1016/j.virs.2025.03.009","url":null,"abstract":"<div><div>Human bocavirus 1 (HBoV1; family: <em>Parvoviridae</em>) causes a wide spectrum of respiratory diseases in children and gastroenteritis in adults. A lack of sensitive cell lines and efficient animal models hinders research on HBoV, including the development of anti-HBoV drugs or vaccines. Although the construction of a wild-type HBoV1 infectious clone has been reported, generating HBoV1 infectious clone carrying foreign reporter genes with suitable insertion sites in its genome while retaining replicative ability remains challenging. Here, HBoV1 infectious clones harboring the 11-amino-acid HiBiT tag at five distinct insertion sites were constructed and evaluated. Only the recombinant HBoV1 carrying the HiBiT tag in the N-terminus of the NS1 protein (HBoV1-HiBiT<sub>NS1</sub>) displayed comparable characteristics to wild-type HBoV1 as determined via the analysis of viral DNA copy number, NanoLuc activity, viral protein expression, and the formation of replication intermediates. Notably, the replication kinetics of HBoV1-HiBiT<sub>NS1</sub> could be examined by monitoring NanoLuc activity, which was noted to be correlated with the viral DNA level. Additionally, we successfully applied HiBiT-tagged HBoV1 for the evaluation of antiviral drug activity and identified ivermectin (EC50 = 2.27 μM) as a potent anti-HBoV1 replication drug. Overall, our study demonstrated that the HBoV1-HiBiT<sub>NS1</sub> reporter can serve as a convenient platform for screening candidate drugs targeting HBoV1 replication and may also be useful for investigating the life cycle of the virus.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 275-283"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4+ T cell 宿主因子 Naf1 限制了 HIV-1 对髓细胞的感染，并削弱了树突状细胞为 CD4+ T 细胞提供能量的能力。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.007

Chengzuo Xie , Xia Jin , Wan-Wei Li , Jian-Hua Wang

{"title":"Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4+ T cell","authors":"Chengzuo Xie , Xia Jin , Wan-Wei Li , Jian-Hua Wang","doi":"10.1016/j.virs.2025.03.007","DOIUrl":"10.1016/j.virs.2025.03.007","url":null,"abstract":"<div><div>Naf1 (Nef-associated factor 1) is a host protein that interacts with human immunodeficiency virus type 1 (HIV-1) Nef protein. We and others have previously demonstrated that Naf1 restricts HIV-1 infection of T-lymphocytes. Myeloid cells are targets for HIV infection, but Naf1 expression in myeloid cells and whether it also regulates HIV infection in these cells are not yet identified. In this study, we found that Naf1 had a higher expression in CD14<sup>+</sup> monocytes than in monocyte-derived dendritic cells (MDDCs), and its expression in both types of cells could be induced by HIV-1 gp120 glycoproteins or viral particles. Importantly, the expression of Naf1 restricted HIV-1 infection in monocytes and MDDCs. Functional investigation showed that both the constitutive and the induced expression of Naf1 inhibited NF-κB signaling in MDDCs and reduced the basal level or LPS (Lipopolysaccharide)-stimulated production of cytokines. Moreover, Naf1 reduced the expression of ICAM-1 (intercellular cell adhesion molecule-1) on MDDCs and compromised their capacity to prime the activation of resting CD4<sup>+</sup> T cells in co-culture. In light of the essential role of NF-κB signaling for HIV-1 transcription, Naf1-mediated inhibition of NF-κB signaling may hinder a robust viral replication in MDDCs and help maintain viral persistence. Furthermore, virus-induced Naf1 expression in MDDCs may diminish the cross-talk between DC (dendritic cell) and T cells, hence suppressing the activation of antiviral immune responses. Taken together, we identified the new function of Naf1 in myeloid cells. Those findings may facilitate the understanding for the host restriction of HIV-1 infection in myeloid cells.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 217-224"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Qingqi Guxue Decoction induces S cell cycle arrest to inhibit replication of severe fever with thrombocytopenia syndrome virus 青芪归脾汤能诱导 S 细胞周期停滞，抑制严重发热伴血小板减少综合征病毒的复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.011

Xixi Shi , Zining Wang , Zixiang Liu , Qinting Lin , Mengqian Huang , Tze Yean Lim , Xiaoyan Li , Tao Wang

{"title":"Qingqi Guxue Decoction induces S cell cycle arrest to inhibit replication of severe fever with thrombocytopenia syndrome virus","authors":"Xixi Shi , Zining Wang , Zixiang Liu , Qinting Lin , Mengqian Huang , Tze Yean Lim , Xiaoyan Li , Tao Wang","doi":"10.1016/j.virs.2025.03.011","DOIUrl":"10.1016/j.virs.2025.03.011","url":null,"abstract":"<div><div>Severe fever with thrombocytopenia syndrome (SFTS) is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus (SFTSV), characterized by high fever and thrombocytopenia. It has been proved that traditional Chinese medicine (TCM) has displayed definite therapeutic effects on viral hemorrhagic fever, indicating its potential to treat SFTS. In this study, SFTS-relative key targets were predicted via gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis. Molecular docking was then used to select stable binders. Molecules matched TCMs were identified, and a new prescription, Qingqi Guxue decoction (QQGX), was formulated to clear heat and nourish blood, with a resulting drug composition network. We explored the optimal drug proportion for QQGX. Through an in-depth study of molecular mechanisms, we found that QQGX induces S phase arrest by promoting the degradation of cyclin A2 (CCNA2) and cyclin-dependent kinase 2 (CDK2), thereby inhibiting SFTSV replication. Finally, we verified the effectiveness and safety of QQGX based on the mouse liver bile duct organoid model infected with SFTSV. In summary, our study prepared a TCM decoction using the method of network pharmacology. This decoction has a significant inhibitory effect on the replication of SFTSV and provides a new treatment strategy for hemorrhagic fever with TCM.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 260-274"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Insights into cross-species infection: Porcine epidemic diarrhea virus infections in the rodent. 冠状病毒跨物种感染的认识：猪流行性腹泻病毒在啮齿动物中的感染。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-03-27 DOI: 10.1016/j.virs.2025.03.012

Jianing Chen, Zemei Wang, Shengyu Lin, Mengling Gao, Yongheng Shao, Shuxian Li, Qingbo Chen, Yaru Cui, Yonghao Hu, Guangliang Liu

{"title":"Insights into cross-species infection: Porcine epidemic diarrhea virus infections in the rodent.","authors":"Jianing Chen, Zemei Wang, Shengyu Lin, Mengling Gao, Yongheng Shao, Shuxian Li, Qingbo Chen, Yaru Cui, Yonghao Hu, Guangliang Liu","doi":"10.1016/j.virs.2025.03.012","DOIUrl":"10.1016/j.virs.2025.03.012","url":null,"abstract":"<p><p>The cross-species infection of coronaviruses has resulted in several major epidemics since 2003. Porcine epidemic diarrhea virus (PEDV) is a devastating swine enteric coronavirus, which targets pigs as the only natural reservoir. In this study, the nucleic acid of PEDV was detected in rat fecal samples collected from pig farms. Further animal tests showed that PEDV can cause systemic infections in neonate mice and rats via intracranial inoculation. The brain, lung intestine and spleen were all targets for PEDV in rodents in contrast to the intestine being targeted in pigs. Morbidity and mortality vary via different infection routes. PEDV was also detectable in feces after infection, suggesting that the infected rodents were potential infectious sources. Moreover, the cerebric tropism of PEDV was verified in piglets via orally inoculation, which had not been identified before. In conclusion, our findings demonstrate that PEDV can cross the species barrier to infect mice and rats through different routes in experimental settings. Although it is highly devastating to piglets, PEDV changes the target organs and turns to be milder when meeting with new hosts. Based on these findings, more attention should be paid to the potential cross-species infection of PEDV to avoid the emergence of another zoonosis.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Development and immunoprotection assessment of novel vaccines for avian infectious bronchitis virus. 禽传染性支气管炎病毒新型疫苗的研制及免疫保护效果评价。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-03-25 DOI: 10.1016/j.virs.2025.03.008

Benli Huang, Sheng Chen, Zhanxin Wang, Keyu Feng, Yutao Teng, Ruoying Li, Guanming Shao, Jiaqian Rao, Xinheng Zhang, Qingmei Xie

{"title":"Development and immunoprotection assessment of novel vaccines for avian infectious bronchitis virus.","authors":"Benli Huang, Sheng Chen, Zhanxin Wang, Keyu Feng, Yutao Teng, Ruoying Li, Guanming Shao, Jiaqian Rao, Xinheng Zhang, Qingmei Xie","doi":"10.1016/j.virs.2025.03.008","DOIUrl":"10.1016/j.virs.2025.03.008","url":null,"abstract":"<p><p>Infectious bronchitis (IB), a highly contagious acute respiratory disease affecting avian species, poses significant challenges to poultry production. The causative agent, Infectious Bronchitis Virus (IBV), exhibits a high mutation rate, leading to limited cross-protection by existing vaccines. This necessitates the development of novel vaccines. This study, based on preliminary investigations conducted by our research team, identified six potential strains (PYG QX1, ZQF QX2, FQH QX3, LYZ QX4, XXX QX5, and CSL strains) for vaccine development. Previous pathogenicity test and serum cross-neutralization experiments conducted in this study have demonstrated that the FQH QX3 strain exhibited the weakest pathogenicity and the broadest spectrum of serum neutralization, while the CSL strain showed the highest pathogenicity and was the most challenging to neutralize, posing the greatest difficulty in prevention and control. Subsequently, we constructed and rescued recombinant vaccine candidates, H120-FQH QX3, and H120-CSL, expressing the S1 and N proteins of the FQH QX3 and CSL strains, respectively. Immunization protection experiments indicated that the H120-CSL recombinant vaccine candidate exhibited the most effective immune protection, making it a promising candidate for further study and evaluation as a recombinant vaccine. The S1 and N genes of the CSL strain demonstrated strong immunogenicity, making them potential candidate antigen genes for future vaccine development.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0