Virologica Sinica最新文献_第5页

Identification of mutations in viral proteins involved in cell adaptation using a reverse genetic system of the live attenuated hepatitis A virus vaccine H2 strain 利用甲型肝炎病毒减毒活疫苗 H2 株的反向遗传系统鉴定参与细胞适应的病毒蛋白变异。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/j.virs.2024.08.004

Xiu-Li Yan , Jian Li , Qing-Qing Ma , Hong-Jiang Wang , Lin Li , Hui Zhao , Cheng-Feng Qin , Xiao-Feng Li

{"title":"Identification of mutations in viral proteins involved in cell adaptation using a reverse genetic system of the live attenuated hepatitis A virus vaccine H2 strain","authors":"Xiu-Li Yan , Jian Li , Qing-Qing Ma , Hong-Jiang Wang , Lin Li , Hui Zhao , Cheng-Feng Qin , Xiao-Feng Li","doi":"10.1016/j.virs.2024.08.004","DOIUrl":"10.1016/j.virs.2024.08.004","url":null,"abstract":"<div><div>The live attenuated hepatitis A virus vaccine H2 strain was developed by passaging a wild-type H2w isolate in cell cultures. Currently, the mechanism underlying its attenuation phenotype remain largely unknown. In this study, we generated a full-length infectious cDNA clone of the H2 strain using in-fusion techniques. The recovered H2 strain (H2ic) from the cDNA clone exhibited an efficient replication in both the hepatoma cell line Huh7.5.1 and the 2BS cell line used for vaccine production, similar to the parental H2 strain. Additionally, H2ic did not cause disease in <em>Ifnar1</em><sup><em>−/−</em></sup> C57 mice, consistent with the H2 strain. To explore the cell-adaptive mutations of the H2 strain, chimeric viruses were generated by replacing its non-structural proteins with corresponding regions from H2w using the infectious cDNA clone as a genetic backbone. The chimeric viruses carrying the 3C or 3D proteins from H2w showed decreased replication in Huh7.5.1 and 2BS cell lines compared to H2ic. Other chimeric viruses containing the 2B, 2C, or 3A proteins from H2w failed to be recovered. Furthermore, there were no significant differences in disease manifestation in mice between H2ic and the recovered chimeric viruses. These results demonstrate that adaptive mutations in the 2B, 2C, and 3A proteins are essential for efficient replication of the H2 strain in cell cultures. Mutations in the 3C and 3D proteins contribute to enhanced replication in cell cultures but did not influence the attenuated phenotypes in mice. Together, this study presents the first reverse genetic system of the H2 strain and identifies viral proteins essential for adaptation to cell cultures.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 6","pages":"Pages 882-891"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11738778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141996602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Ten computational challenges in human virome studies 人类病毒组研究中的十大计算挑战。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/j.virs.2024.04.008

Yifan Wu, Yousong Peng

{"title":"Ten computational challenges in human virome studies","authors":"Yifan Wu, Yousong Peng","doi":"10.1016/j.virs.2024.04.008","DOIUrl":"10.1016/j.virs.2024.04.008","url":null,"abstract":"<div><div>In recent years, substantial advancements have been achieved in understanding the diversity of the human virome and its intricate roles in human health and diseases. Despite this progress, the field of human virome research remains nascent, primarily hindered by the lack of effective methods, particularly in the domain of computational tools. This perspective systematically outlines ten computational challenges spanning various types of virome studies. These challenges arise due to the vast diversity of viromes, the absence of a universal marker gene in viral genomes, the low abundance of virus populations, the remote or minimal homology of viral proteins to known proteins, and the highly dynamic and heterogeneous nature of viromes. For each computational challenge, we discuss the underlying reasons, current research progress, and potential solutions. The resolution of these challenges necessitates ongoing collaboration among computational scientists, virologists, and multidisciplinary experts. In essence, this perspective serves as a comprehensive guide for directing computational efforts in human virome studies.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 6","pages":"Pages 845-850"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11738758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The first discovery of severe fever with thrombocytopenia virus in the center of metropolitan Beijing, China 在中国北京市中心首次发现严重发热伴血小板减少病毒。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/j.virs.2024.11.002

Fei Yuan , Lianglong Zhu , Di Tian , Mengyu Xia , Ming-hao Zheng , Qing Zhang , Tingyu Zhang , Xing Zhang , Aihua Zheng

{"title":"The first discovery of severe fever with thrombocytopenia virus in the center of metropolitan Beijing, China","authors":"Fei Yuan , Lianglong Zhu , Di Tian , Mengyu Xia , Ming-hao Zheng , Qing Zhang , Tingyu Zhang , Xing Zhang , Aihua Zheng","doi":"10.1016/j.virs.2024.11.002","DOIUrl":"10.1016/j.virs.2024.11.002","url":null,"abstract":"<div><div>Severe fever with thrombocytopenia virus (SFTSV), an emerging tick-borne bandavirus, poses a significant public health threat in rural China. Since 2021, an increase of local cases has been noted in the rural-urban fringe of Beijing. This study aimed to assess the formation of natural foci in urban areas by conducting a field survey of ticks and hedgehogs from the second to fifth ring roads of Beijing. Our survey revealed a diverse tick population in city parks, including the major SFTSV vector, <em>Haemaphysalis longicornis</em>. Parthenogenetic <em>H. longicornis</em>, known for its role in the rapid spread of SFTSV, was identified in key locations such as Beihai Park and Taoranting Park, near the Forbidden City. Notably, high SFTSV seroprevalence and RNA prevalence were found in hedgehogs and parasitic ticks in the center of Beijing. Phylogenetic analyses of SFTSV RNA and mitochondrial sequences of parthenogenetic <em>H. longicornis</em> ticks revealed the existence of diverse lineages of SFTSV and <em>H. longicornis</em> ticks within Beijing, suggesting multiple invasion events happened. These findings reveal the circulation of SFTSV in central Beijing, highlighting the need for urgent attention and enhanced surveillance measures.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 6","pages":"Pages 875-881"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11738777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Issue Cover 覆盖问题

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/S1995-820X(24)00187-1

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Efficient and robust reverse genetics system for bovine rotavirus generation and its application for antiviral screening 用于生成牛轮状病毒的高效、稳健的反向遗传学系统及其在抗病毒筛选中的应用。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/j.virs.2024.09.010

Song-Kang Qin , Kuan-Hao Li , Ben-Jin Liu , Cun Cao , De-Bin Yu , Zhi-Gang Jiang , Jun Wang , Yu-Xin Han , Fang Wang , Ying-Lin Qi , Chao Sun , Li Yu , Ji-Tao Chang , Xin Yin

{"title":"Efficient and robust reverse genetics system for bovine rotavirus generation and its application for antiviral screening","authors":"Song-Kang Qin , Kuan-Hao Li , Ben-Jin Liu , Cun Cao , De-Bin Yu , Zhi-Gang Jiang , Jun Wang , Yu-Xin Han , Fang Wang , Ying-Lin Qi , Chao Sun , Li Yu , Ji-Tao Chang , Xin Yin","doi":"10.1016/j.virs.2024.09.010","DOIUrl":"10.1016/j.virs.2024.09.010","url":null,"abstract":"<div><div>Unveiling the molecular mechanisms underlying rotavirus replication and pathogenesis has been hampered by the lack of a reverse genetics (RG) system in the past. Since 2017, multiple plasmid-based RG systems for simian, human, and murine-like rotaviruses have been established. However, none of the described methods have supported the recovery of bovine rotaviruses (BRVs). Here, we established an optimized plasmid-based RG system for BRV culture-adapted strain (BRV G10P [15] BLR) and clinical isolates (BRV G6P [1] C73, G10P [11] HM26) based on a BHK-T7 cell clone stably expressing T7 polymerase. Furthermore, using this optimized RG system, we successfully rescued the reporter virus BRV rC73/Zs, rHM26/Zs and rBLR/Zs, harboring a genetically modified 1.8-kb segment 7 encoding full-length nonstructural protein 3 (NSP3) fused to ZsGreen, a 232-amino acid green fluorescent protein. Analysis of the stability of genomic insertions showed that the rC73/Zs and rBLR/Zs replicated efficiently and were genetically stable in seven rounds of serial passaging, while rHM26/Zs can be stabilized only up to the third generation, indicating that the BRV segment composition may influence the viral fitness. In addition, we adopted the recombinant reporter viruses for high-throughput screening application and discovered 12 candidates out of 1440 compounds with potential antiviral activities against rotavirus. In summary, this improved RG system of BRVs represents an important tool with great potential for understanding the molecular biology of BRV and facilitates the development of novel therapeutics and vaccines for BRV.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 6","pages":"Pages 917-928"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11738791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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2024 Reviewer Acknowledgment 2024审稿人致谢

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/S1995-820X(24)00202-5

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Bis-benzylisoquinoline alkaloids inhibit flavivirus entry and replication by compromising endolysosomal trafficking and autophagy 双苄基异喹啉生物碱通过影响内溶酶体转运和自噬抑制黄病毒的进入和复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/j.virs.2024.09.001

Lihong Huang , Lele Liu , Junhai Zhu , Nanjun Chen , Jie Chen , Chuen-Fuk Chan , Fei Gao , Youqin Yin , Jiufeng Sun , Rongxin Zhang , Kehui Zhang , Wenbao Qi , Jianbo Yue

{"title":"Bis-benzylisoquinoline alkaloids inhibit flavivirus entry and replication by compromising endolysosomal trafficking and autophagy","authors":"Lihong Huang , Lele Liu , Junhai Zhu , Nanjun Chen , Jie Chen , Chuen-Fuk Chan , Fei Gao , Youqin Yin , Jiufeng Sun , Rongxin Zhang , Kehui Zhang , Wenbao Qi , Jianbo Yue","doi":"10.1016/j.virs.2024.09.001","DOIUrl":"10.1016/j.virs.2024.09.001","url":null,"abstract":"<div><div>Flaviviruses, such as dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV), represent a substantial public health challenge as there are currently no approved treatments available. Here, we investigated the antiviral effects of bis-benzylisoquinoline alkaloids (BBAs) on flavivirus infections. We evaluated five specific BBAs—berbamine, tetrandrine, iso-tetrandrine, fangchinoline, and cepharanthine—and found that they effectively inhibited infections by ZIKV, DENV, or JEV by blocking virus entry and genome replication stages in the flavivirus life cycle. Furthermore, we synthesized a fluorophore-conjugated BBA and showed that BBAs targeted endolysosomes, causing lysosomal pH alkalization. Mechanistic studies on inhibiting ZIKV infection by BBAs revealed that these compounds blocked TRPML channels, leading to lysosomal dysfunction and reducing the expression of NCAM1, a key receptor for the entry of ZIKV into cells, thereby decreasing cells susceptibility to ZIKV infection. Additionally, BBAs inhibited the fusion of autophagosomes and lysosomes, significantly reducing viral RNA replication. Collectively, our results suggest that BBAs inhibit flavivirus entry and replication by compromising endolysosomal trafficking and autophagy, respectively, underscoring the potential of BBAs as therapeutic agents against flavivirus infections.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 6","pages":"Pages 892-908"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142180133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A G3P[3] bat rotavirus can infect cultured human cholangiocytes and cause biliary atresia symptom in suckling mice G3P[3]蝙蝠轮状病毒可感染培养的人类胆管细胞，并导致乳鼠出现胆道闭锁症状。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/j.virs.2024.09.009

Pengfei Hao , Zhaoxia Pang , Qiaoqiao Qu , Chunmei Cui , Yuhang Jiang , Jing Chen , Zihan Gao , Zhiqiang Xu , Letian Li , Ningyi Jin , Chang Li

引用次数: 0

Generation and characterization of a novel ovariole cell line derived from Spodoptera frugiperda in China with sensitivity to both SfMNPV and AcMNPV 产生并鉴定对 SfMNPV 和 AcMNPV 均敏感的新型卵巢细胞系。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/j.virs.2024.10.002

Yan Tong , Wenyi Jin , Xuan Li , Lin Guo , Gang Luo , Qian Meng , Jihong Zhang , Qilian Qin , Huan Zhang

{"title":"Generation and characterization of a novel ovariole cell line derived from Spodoptera frugiperda in China with sensitivity to both SfMNPV and AcMNPV","authors":"Yan Tong , Wenyi Jin , Xuan Li , Lin Guo , Gang Luo , Qian Meng , Jihong Zhang , Qilian Qin , Huan Zhang","doi":"10.1016/j.virs.2024.10.002","DOIUrl":"10.1016/j.virs.2024.10.002","url":null,"abstract":"<div><div><em><em>Spodoptera frugiperda</em></em> multiple nucleopolyhedrovirus (SfMNPV), belonging to the species <em>Alphabaculovirus spofrugiperdae</em>, has been recently registered as an insecticide in China. This virus has a specific effect on the global major agricultural pest <em>Spodoptera frugiperda</em>. To gain insights into viral infection, replication processes, and the complex formation of viral particles, <em>in vitro</em> studies using cell lines are essential tools. Although the IPLB-Sf9 and IPLB-Sf21 cell lines derived from <em>S. frugiperda</em> are widely used for studies on the infection and replication mechanisms of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), their capacity to produce viral polyhedra after SfMNPV infection is not optimal. To address this limitation, a novel cell line named IOZCAS-Sf-1 was developed from a <em>S. frugiperda</em> population in Yunnan, China. The mitochondrial <em>COX1</em> gene analysis confirmed the species origin of the IOZCAS-Sf-1 cell line. Furthermore, a comparative study was carried out to contrast the <em>COX1</em> gene sequence of this novel cell line with that of IPLB-Sf9, highlighting the distinctions between the two. Importantly, the IOZCAS-Sf-1 cells exhibited a remarkable ability to generate polyhedra when infected with AcMNPV and SfMNPV, respectively. Consequently, this cellular lineage is considered a promising and valuable resource. It serves not only to investigate the molecular mechanisms of viral replication and its impact on host cells, but also to explore the transfection efficiency of SfMNPV DNA. This exploration further expands into its potential application in recombinant DNA experiments, laying a theoretical groundwork for the advancement of more effective biopesticides and sustainable agricultural practices.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 6","pages":"Pages 929-937"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11738775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case study: May human norovirus infection be associated with premature delivery? 病例研究：人类诺如病毒感染可能与早产有关吗？

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-12-01 DOI: 10.1016/j.virs.2024.10.004

Jiaying Cao , Yuetong Li , Feiyang Xue , Ziyang Sheng , Libo Liu , Yingying Zhang , Lele Wang , Liang Zeng , Yanmin Jiang , Dongying Fan , Fang Li , Jing An

{"title":"Case study: May human norovirus infection be associated with premature delivery?","authors":"Jiaying Cao , Yuetong Li , Feiyang Xue , Ziyang Sheng , Libo Liu , Yingying Zhang , Lele Wang , Liang Zeng , Yanmin Jiang , Dongying Fan , Fang Li , Jing An","doi":"10.1016/j.virs.2024.10.004","DOIUrl":"10.1016/j.virs.2024.10.004","url":null,"abstract":"<div><div>Human norovirus (HuNoV) is the leading cause of acute gastroenteritis. The varying severity of chronic infection in patients with underlying immune deficiencies poses additional burdens on public health. However, the potential effects of HuNoV infection during pregnancy, a specific immune perturbed state, have been rarely reported. Recently, four cases of HuNoV-infected patients in the late stages of pregnancy were admitted to the Guangzhou Women and Children's Medical Center, and premature rupture of membranes as primary adverse outcome was observed in these cases. Samples of fetal accessory tissue were collected from two of these cases at delivery to explore the potential pathogenesis. Pathological analysis showed placental malperfusion in both maternal and fetal vascular, while a decrease in vessels was not observed in villi of placenta. There was obvious pathological change in the chorion of fetal membrane, accompanied by a tendency of Th-1 immune bias. Notably, aggregation of M2 macrophages was observed in the chorion of the fetal membrane, potentially recruited for tissue repair. Next-generation sequencing showed minimal changes in immune pathways within placenta tissue. A gene panel associated with immunosuppression was identified in the fetal membrane of HuNoV-infected women compared to those of normal parturient. Taken together, this study provides clues for the association between the HuNoV and premature delivery, which requires the attention of the clinicians.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 6","pages":"Pages 951-962"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11738789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0