Virologica Sinica最新文献_第6页

Artemisia annua L. leaf extracts suppress influenza virus infection by targeting the viral nucleoprotein and blocking mitochondria-mediated apoptosis 黄花蒿叶提取物通过靶向病毒核蛋白和阻断线粒体介导的细胞凋亡抑制流感病毒感染。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.001

Xiwen Zhao , Xuan Dai , Fuyi Wang , Chenyang Li , Xun Song , Yingying Han , Chaowei Zhang , Lu Wang , Zhendan He , Rongping Zhang , Liang Ye

{"title":"Artemisia annua L. leaf extracts suppress influenza virus infection by targeting the viral nucleoprotein and blocking mitochondria-mediated apoptosis","authors":"Xiwen Zhao , Xuan Dai , Fuyi Wang , Chenyang Li , Xun Song , Yingying Han , Chaowei Zhang , Lu Wang , Zhendan He , Rongping Zhang , Liang Ye","doi":"10.1016/j.virs.2025.03.001","DOIUrl":"10.1016/j.virs.2025.03.001","url":null,"abstract":"<div><div><em>Artemisia annua</em> L. is a medicinal herb with multiple therapeutic applications, whereas its anti-influenza A virus (IAV) efficiency and mechanism of action are still unclear. Here, we investigated the inhibition activity and mechanism of <em>A. annua</em> leaf methanol extracts (AALME) against IAV <em>in vitro</em> and <em>in vivo</em>. Our results revealed that AALME exhibits potent anti-IAV activity by interacting with IAV particles. Mechanistically, AALME directly targets the IAV nucleoprotein (NP) protein and abolishes the nuclear import of IAV NP. AALME profoundly suppresses IAV-induced mitochondrial apoptosis via suppressing ROS-mediated AIF-dependent pathways. More importantly, we found that AALME plays a crucial role in protecting mice from IAV infection and mitigating IAV pathogenicity. This current work provides mechanistic insight into the mechanism by which AALME controls IAV infection <em>in vitro</em> and <em>in vivo</em>, potentially contributing to the development of antiviral treatments for IAV infection.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 247-259"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of novel highly pathogenic avian influenza A(H5N6) clade 2.3.4.4b virus in wild birds, East China, 2024 中国东部地区野生鸟类新型高致病性禽流感A（H5N6）分支2.3.4.4b病毒特征分析

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.01.002

Renjie Sun , Xiaoxiao Feng , Jing Huang , Fangyu Zheng , Ronghui Xie , Chuanliang Zhang , Hongli Zhang , Yin Xue , Aijun Liu , Xiaobing Huang , Lin Yuan , Lingyan Zhao

引用次数: 0

Identification of an immunodominant neutralizing epitope of porcine astrovirus type 5 capsid protein 猪星状病毒5型衣壳蛋白免疫显性中和表位的鉴定。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.003

Yixin Hu , Zehui Li , Chenlin Hao , Hao Lu , Yunfei Xing , Kexin Liu , Xiaohui Jin , Zhanyong Wei

引用次数: 0

Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies 用于抗病毒药物筛选和生命周期研究的报告毒株HBoV1的研制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.009

Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen

{"title":"Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies","authors":"Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen","doi":"10.1016/j.virs.2025.03.009","DOIUrl":"10.1016/j.virs.2025.03.009","url":null,"abstract":"<div><div>Human bocavirus 1 (HBoV1; family: <em>Parvoviridae</em>) causes a wide spectrum of respiratory diseases in children and gastroenteritis in adults. A lack of sensitive cell lines and efficient animal models hinders research on HBoV, including the development of anti-HBoV drugs or vaccines. Although the construction of a wild-type HBoV1 infectious clone has been reported, generating HBoV1 infectious clone carrying foreign reporter genes with suitable insertion sites in its genome while retaining replicative ability remains challenging. Here, HBoV1 infectious clones harboring the 11-amino-acid HiBiT tag at five distinct insertion sites were constructed and evaluated. Only the recombinant HBoV1 carrying the HiBiT tag in the N-terminus of the NS1 protein (HBoV1-HiBiT<sub>NS1</sub>) displayed comparable characteristics to wild-type HBoV1 as determined via the analysis of viral DNA copy number, NanoLuc activity, viral protein expression, and the formation of replication intermediates. Notably, the replication kinetics of HBoV1-HiBiT<sub>NS1</sub> could be examined by monitoring NanoLuc activity, which was noted to be correlated with the viral DNA level. Additionally, we successfully applied HiBiT-tagged HBoV1 for the evaluation of antiviral drug activity and identified ivermectin (EC50 = 2.27 μM) as a potent anti-HBoV1 replication drug. Overall, our study demonstrated that the HBoV1-HiBiT<sub>NS1</sub> reporter can serve as a convenient platform for screening candidate drugs targeting HBoV1 replication and may also be useful for investigating the life cycle of the virus.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 275-283"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4+ T cell 宿主因子 Naf1 限制了 HIV-1 对髓细胞的感染，并削弱了树突状细胞为 CD4+ T 细胞提供能量的能力。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.007

Chengzuo Xie , Xia Jin , Wan-Wei Li , Jian-Hua Wang

{"title":"Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4+ T cell","authors":"Chengzuo Xie , Xia Jin , Wan-Wei Li , Jian-Hua Wang","doi":"10.1016/j.virs.2025.03.007","DOIUrl":"10.1016/j.virs.2025.03.007","url":null,"abstract":"<div><div>Naf1 (Nef-associated factor 1) is a host protein that interacts with human immunodeficiency virus type 1 (HIV-1) Nef protein. We and others have previously demonstrated that Naf1 restricts HIV-1 infection of T-lymphocytes. Myeloid cells are targets for HIV infection, but Naf1 expression in myeloid cells and whether it also regulates HIV infection in these cells are not yet identified. In this study, we found that Naf1 had a higher expression in CD14<sup>+</sup> monocytes than in monocyte-derived dendritic cells (MDDCs), and its expression in both types of cells could be induced by HIV-1 gp120 glycoproteins or viral particles. Importantly, the expression of Naf1 restricted HIV-1 infection in monocytes and MDDCs. Functional investigation showed that both the constitutive and the induced expression of Naf1 inhibited NF-κB signaling in MDDCs and reduced the basal level or LPS (Lipopolysaccharide)-stimulated production of cytokines. Moreover, Naf1 reduced the expression of ICAM-1 (intercellular cell adhesion molecule-1) on MDDCs and compromised their capacity to prime the activation of resting CD4<sup>+</sup> T cells in co-culture. In light of the essential role of NF-κB signaling for HIV-1 transcription, Naf1-mediated inhibition of NF-κB signaling may hinder a robust viral replication in MDDCs and help maintain viral persistence. Furthermore, virus-induced Naf1 expression in MDDCs may diminish the cross-talk between DC (dendritic cell) and T cells, hence suppressing the activation of antiviral immune responses. Taken together, we identified the new function of Naf1 in myeloid cells. Those findings may facilitate the understanding for the host restriction of HIV-1 infection in myeloid cells.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 217-224"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qingqi Guxue Decoction induces S cell cycle arrest to inhibit replication of severe fever with thrombocytopenia syndrome virus 青芪归脾汤能诱导 S 细胞周期停滞，抑制严重发热伴血小板减少综合征病毒的复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.011

Xixi Shi , Zining Wang , Zixiang Liu , Qinting Lin , Mengqian Huang , Tze Yean Lim , Xiaoyan Li , Tao Wang

{"title":"Qingqi Guxue Decoction induces S cell cycle arrest to inhibit replication of severe fever with thrombocytopenia syndrome virus","authors":"Xixi Shi , Zining Wang , Zixiang Liu , Qinting Lin , Mengqian Huang , Tze Yean Lim , Xiaoyan Li , Tao Wang","doi":"10.1016/j.virs.2025.03.011","DOIUrl":"10.1016/j.virs.2025.03.011","url":null,"abstract":"<div><div>Severe fever with thrombocytopenia syndrome (SFTS) is a novel emerging acute infectious disease caused by severe fever with thrombocytopenia syndrome virus (SFTSV), characterized by high fever and thrombocytopenia. It has been proved that traditional Chinese medicine (TCM) has displayed definite therapeutic effects on viral hemorrhagic fever, indicating its potential to treat SFTS. In this study, SFTS-relative key targets were predicted via gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis. Molecular docking was then used to select stable binders. Molecules matched TCMs were identified, and a new prescription, Qingqi Guxue decoction (QQGX), was formulated to clear heat and nourish blood, with a resulting drug composition network. We explored the optimal drug proportion for QQGX. Through an in-depth study of molecular mechanisms, we found that QQGX induces S phase arrest by promoting the degradation of cyclin A2 (CCNA2) and cyclin-dependent kinase 2 (CDK2), thereby inhibiting SFTSV replication. Finally, we verified the effectiveness and safety of QQGX based on the mouse liver bile duct organoid model infected with SFTSV. In summary, our study prepared a TCM decoction using the method of network pharmacology. This decoction has a significant inhibitory effect on the replication of SFTSV and provides a new treatment strategy for hemorrhagic fever with TCM.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 260-274"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into recent advancements in human and animal rotavirus vaccines: Exploring new frontiers 人类和动物轮状病毒疫苗的最新进展：探索新领域。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.001

Ahmed H. Ghonaim , Sherin R. Rouby , Wedad M. Nageeb , Ashraf Ahmed Elgendy , Rong Xu , Changsheng Jiang , Noha H. Ghonaim , Qigai He , Wentao Li

引用次数: 0

Distinct tropisms of HCMV and SARS-CoV-2 in lung tissue of a patient with advanced HIV disease 晚期HIV患者肺组织中HCMV和SARS-CoV-2的明显趋向性

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2025.01.001

Jingjing Xu , Bo Yang , Ye Zheng , Yuexiang Yang , Min-Hua Luo , Yun Ling , Xiaohong Fan , Han Cheng

引用次数: 0

A low pathogenic avian influenza A/Mallard/South Korea/KNU2019-34/2019 (H1N1) virus has the potential to increase the mammalian pathogenicity 低致病性禽流感A/野鸭/韩国/KNU2019-34/2019 （H1N1）病毒具有提高哺乳动物致病性的潜力。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.005

Jaemoo Kim , Jungho Kim , Suhyeon Heo , Chang-Hun Yeom , Bao Tuan Duong , Haan Woo Sung , Seon-Ju Yeo , Hyun Park , Haryoung Poo , Jihyun Yang

{"title":"A low pathogenic avian influenza A/Mallard/South Korea/KNU2019-34/2019 (H1N1) virus has the potential to increase the mammalian pathogenicity","authors":"Jaemoo Kim , Jungho Kim , Suhyeon Heo , Chang-Hun Yeom , Bao Tuan Duong , Haan Woo Sung , Seon-Ju Yeo , Hyun Park , Haryoung Poo , Jihyun Yang","doi":"10.1016/j.virs.2024.12.005","DOIUrl":"10.1016/j.virs.2024.12.005","url":null,"abstract":"<div><div>Influenza, a highly contagious respiratory infectious disease caused by an influenza virus, is a threat to public health worldwide. Avian influenza viruses (AIVs) have the potential to cause the next pandemic by crossing the species barrier through mutation of viral genome. Here, we investigated the pathogenicity of AIVs obtained from South Korea and Mongolia during 2018–2019 by measuring viral titers in the lungs and extrapulmonary organs of mouse models. In addition, we assessed the pathogenicity of AIVs in ferret models. Moreover, we compared the ability of viruses to replicate in mammalian cells, as well as the receptor-binding preferences of AIV isolates. Genetic analyses were finally performed to identify the genetic relationships and amino acid substitutions between viral proteins during mammalian adaptation. Of the 24 AIV isolates tested, A/Mallard/South Korea/KNU2019-34/2019 (KNU19-34; H1N1) caused severe bodyweight loss and high mortality in mice. The virus replicated in the lungs, kidneys, and heart. Importantly, KNU19-34-infected ferrets showed high viral loads in both nasal washes and lungs. KNU19-34 replicated rapidly in A549 and bound preferentially to human like α2,6-linked sialic acids rather than to avian-like α2,3-linked sialic acids, similar to the pandemic A/California/04/2009 (H1N1) strain. Gene segments of KNU19-34 were distributed in Egypt and Asia lineages from 2015 to 2018, and the virus had several amino acid substitutions compared to H1N1 AIV isolates that were non-pathogenic in mice. Collectively, the data suggest that KNU19-34 has zoonotic potential and the possibility of new mutations responsible for mammalian adaptation.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 24-34"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential mechanisms and material basis of Fuzheng Jiedu decoction broad-spectrum inhibiting coronaviruses 扶正解毒汤广谱抑制冠状病毒的潜在机制和物质基础。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.007

Ke Liu , Bixia Hong , Shi-Ting He , Siying Du , Jiayi Ke , Lili Tian , Tao Tao , Yihan Zhang , Kelin Li , Han Chang , Mengzhe Li , Xiaoping An , Lihua Song , Zhongde Zhang , Liang Liu , Hudan Pan , Huahao Fan , Yigang Tong

{"title":"The potential mechanisms and material basis of Fuzheng Jiedu decoction broad-spectrum inhibiting coronaviruses","authors":"Ke Liu , Bixia Hong , Shi-Ting He , Siying Du , Jiayi Ke , Lili Tian , Tao Tao , Yihan Zhang , Kelin Li , Han Chang , Mengzhe Li , Xiaoping An , Lihua Song , Zhongde Zhang , Liang Liu , Hudan Pan , Huahao Fan , Yigang Tong","doi":"10.1016/j.virs.2024.12.007","DOIUrl":"10.1016/j.virs.2024.12.007","url":null,"abstract":"<div><div>Traditional Chinese medicine has unique advantages in preventing and treating COVID-19, and Fuzheng Jiedu decoction (FZJDD) was reported to be effective against COVID-19 in clinical trials. To investigate the potential mechanisms and material basis of FZJDD against SARS-CoV-2, we performed SARS-CoV-2 target protein inhibition analyses and a metabolite full spectrum analysis of FZJDD. Interestingly, FZJDD was found to block the binding of SARS-CoV-2 Spike protein with the receptor ACE2 and inhibit the activity of SARS-CoV-2 3CLpro. Moreover, FZJDD can regulate the TNF and the MAPK signaling pathway to inhibit the inflammatory response and alleviate the “cytokine storm”. A total of 298 compounds were identified in FZJDD, among them, caffeic acid and octyl gallate were found to be the potential therapeutic agents of FZJDD. Importantly, FZJDD can broadly inhibit coronavirus infection, including SADS-CoV and porcine epidemic diarrhea virus (PEDV) live viruses, SARS-CoV, MERS-CoV, and SARS-CoV-2 mutant pseudotyped viruses, which might be ascribed to the broad-spectrum anti-coronavirus activity of caffeic acid and octyl gallate. In conclusion, this study reveals the mechanisms and material basis of FZJDD against SARS-CoV-2 and identifies the broad-spectrum anti-coronavirus activity of FZJDD for the first time. Our data provide empirical evidence for the development and application of FZJDD.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 125-136"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0