Virologica Sinica最新文献

{"title":"Issue Cover","authors":"","doi":"10.1016/S1995-820X(24)00118-4","DOIUrl":"10.1016/S1995-820X(24)00118-4","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Page OFC"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24001184/pdfft?md5=f0fc735f524512f457333cde1dd9e308&pid=1-s2.0-S1995820X24001184-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142040193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"三源重组H9N2亚型禽流感病毒NA蛋白原核表达及多克隆抗体制备","authors":"梁志鹏 | 刘静 | 刘闰栀 | 张新宇 | 何杰珩 | 陈高婕 | 闫战飞 | 池仕红 | 袁生 | 郭锦玥 | 梁昭平 | 黄淑坚 | 温峰","doi":"10.13350/j.cjpb.240602","DOIUrl":"https://doi.org/10.13350/j.cjpb.240602","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"1 1","pages":""},"PeriodicalIF":5.5,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142033526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary dynamics and comparative pathogenicity of clade 2.3.4.4b H5 subtype avian influenza viruses, China, 2021–2022","authors":"Siru Lin ,&nbsp;Junhong Chen ,&nbsp;Ke Li ,&nbsp;Yang Liu ,&nbsp;Siyuan Fu ,&nbsp;Shumin Xie ,&nbsp;Aimin Zha ,&nbsp;Aiguo Xin ,&nbsp;Xinyu Han ,&nbsp;Yuting Shi ,&nbsp;Lingyu Xu ,&nbsp;Ming Liao ,&nbsp;Weixin Jia","doi":"10.1016/j.virs.2024.04.004","DOIUrl":"10.1016/j.virs.2024.04.004","url":null,"abstract":"<div><p>The recent concurrent emergence of H5N1, H5N6, and H5N8 avian influenza viruses (AIVs) has led to significant avian mortality globally. Since 2020, frequent human-animal interactions have been documented. To gain insight into the novel H5 subtype AIVs (i.e., H5N1, H5N6 and H5N8), we collected 6102 samples from various regions of China between January 2021 and September 2022, and identified 41 H5Nx strains. Comparative analyses on the evolution and biological properties of these isolates were conducted. Phylogenetic analysis revealed that the 41 H5Nx strains belonged to clade 2.3.4.4b, with 13 related to H5N1, 19 to H5N6, and 9 to H5N8. Analysis based on global 2.3.4.4b viruses showed that all the viruses described in this study were likely originated from H5N8, exhibiting a heterogeneous evolutionary history between H5N1 and H5N6 during 2015–2022 worldwide. H5N1 showed a higher rate of evolution in 2021–2022 and more sites under positive selection pressure in 2015–2022. The antigenic profiles of the novel H5N1 and H5N6 exhibited notable variations. Further hemagglutination inhibition assay suggested that some A(H5N1) viruses may be antigenically distinct from the circulating H5N6 and H5N8 strains. Mammalian challenge assays demonstrated that the H5N8 virus (21GD001_H5N8) displayed the highest pathogenicity in mice, followed by the H5N1 virus (B1557_H5N1) and then the H5N6 virus (220086_H5N6), suggesting a heterogeneous virulence profile of H5 AIVs in the mammalian hosts. Based on the above results, we speculate that A(H5N1) viruses have a higher risk of emergence in the future. Collectively, these findings unveil a new landscape of different evolutionary history and biological characteristics of novel H5 AIVs in clade 2.3.4.4b, contributing to a better understanding of designing more effective strategies for the prevention and control of novel H5 AIVs.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 358-368"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000609/pdfft?md5=55771d05289f2fa1c8f2fa8be3ff6474&pid=1-s2.0-S1995820X24000609-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140870073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characteristics of H1N1 influenza virus outbreak in China in early 2023","authors":"Xuanxuan Li ,&nbsp;Zefeng Dong ,&nbsp;Jiaming Li ,&nbsp;Chuanran Dou ,&nbsp;Deyu Tian ,&nbsp;Zhenghai Ma ,&nbsp;Wenjun Liu ,&nbsp;George F. Gao ,&nbsp;Yuhai Bi","doi":"10.1016/j.virs.2024.05.003","DOIUrl":"10.1016/j.virs.2024.05.003","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 520-523"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000713/pdfft?md5=5e7d860fd37043753d5714377e6f507a&pid=1-s2.0-S1995820X24000713-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A lethal mice model of recombinant vesicular stomatitis viruses for EBOV-targeting prophylactic vaccines evaluation","authors":"Hong-Qing Zhang ,&nbsp;Zhe-Rui Zhang ,&nbsp;Cheng-Lin Deng ,&nbsp;Zhi-Ming Yuan ,&nbsp;Bo Zhang","doi":"10.1016/j.virs.2024.03.008","DOIUrl":"10.1016/j.virs.2024.03.008","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 516-519"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000348/pdfft?md5=6d5d11ef9423a2811424692e01ebb0af&pid=1-s2.0-S1995820X24000348-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA vaccine prime and replicating vaccinia vaccine boost induce robust humoral and cellular immune responses against MERS-CoV in mice","authors":"Xiuli Shen ,&nbsp;Shuhui Wang ,&nbsp;Yanling Hao ,&nbsp;Yuyu Fu ,&nbsp;Li Ren ,&nbsp;Dan Li ,&nbsp;Wenqi Tang ,&nbsp;Jing Li ,&nbsp;Ran Chen ,&nbsp;Meiling Zhu ,&nbsp;Shuo Wang ,&nbsp;Ying Liu ,&nbsp;Yiming Shao","doi":"10.1016/j.virs.2024.05.005","DOIUrl":"10.1016/j.virs.2024.05.005","url":null,"abstract":"<div><p>As of December 2022, 2603 laboratory-identified Middle East respiratory syndrome coronavirus (MERS-CoV) infections and 935 associated deaths, with a mortality rate of 36%, had been reported to the World Health Organization (WHO). However, there are still no vaccines for MERS-CoV, which makes the prevention and control of MERS-CoV difficult. In this study, we generated two DNA vaccine candidates by integrating MERS-CoV Spike (S) gene into a replicating Vaccinia Tian Tan (VTT) vector. Compared to homologous immunization with either vaccine, mice immunized with DNA vaccine prime and VTT vaccine boost exhibited much stronger and durable humoral and cellular immune responses. The immunized mice produced robust binding antibodies and broad neutralizing antibodies against the EMC2012, England1 and KNIH strains of MERS-CoV. Prime-Boost immunization also induced strong MERS-S specific T cells responses, with high memory and poly-functional (CD107a-IFN-γ-TNF-α) effector CD8⁺ T cells. In conclusion, the research demonstrated that DNA-Prime/VTT-Boost strategy could elicit robust and balanced humoral and cellular immune responses against MERS-CoV-S. This study not only provides a promising set of MERS-CoV vaccine candidates, but also proposes a heterologous sequential immunization strategy worthy of further development.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 490-500"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000737/pdfft?md5=3ca4c6f75b7b9ef5ed65e0243050da7a&pid=1-s2.0-S1995820X24000737-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Měnglà virus minigenome and comparison of its polymerase complexes with those of other filoviruses","authors":"Shi-Zhe Xie ,&nbsp;Ke Yao ,&nbsp;Bei Li ,&nbsp;Cheng Peng ,&nbsp;Xing-Lou Yang ,&nbsp;Zheng-Li Shi","doi":"10.1016/j.virs.2024.03.011","DOIUrl":"10.1016/j.virs.2024.03.011","url":null,"abstract":"<div><p>Ebola virus (EBOV) and Marburg virus (MARV), members of the <em>Filoviridae</em> family, are highly pathogenic and can cause hemorrhagic fevers, significantly impacting human society. Bats are considered reservoirs of these viruses because related filoviruses have been discovered in bats. However, due to the requirement for maximum containment laboratories when studying infectious viruses, the characterization of bat filoviruses often relies on pseudoviruses and minigenome systems. In this study, we used RACE technology to sequence the 3′-leader and 5′-trailer of Měnglà virus (MLAV) and constructed a minigenome. Similar to MARV, the transcription activities of the MLAV minigenome are independent of VP30. We further assessed the effects of polymorphisms at the 5′ end on MLAV minigenome activity and identified certain mutations that decrease minigenome reporter efficiency, probably due to alterations in the RNA secondary structure. The reporter activity upon recombination of the 3′-leaders and 5′-trailers of MLAV, MARV, and EBOV with those of the homologous or heterologous minigenomes was compared and it was found that the polymerase complex and leader and trailer sequences exhibit intrinsic specificities. Additionally, we investigated whether the polymerase complex proteins from EBOV and MARV support MLAV minigenome RNA synthesis and found that the homologous system is more efficient than the heterologous system. Remdesivir efficiently inhibited MLAV as well as EBOV replication. In summary, this study provides new information on bat filoviruses and the minigenome will be a useful tool for high-throughput antiviral drug screening.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 459-468"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000749/pdfft?md5=2357e0526a9fe81541127d0c038b1b59&pid=1-s2.0-S1995820X24000749-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Cover","authors":"","doi":"10.1016/S1995-820X(24)00085-3","DOIUrl":"https://doi.org/10.1016/S1995-820X(24)00085-3","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Page OFC"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000853/pdfft?md5=26f94952feb3f2209397ff4474b72914&pid=1-s2.0-S1995820X24000853-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141480961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of HiBiT into enteroviruses: A universal tool for advancing enterovirus virology research","authors":"Rui Yu ,&nbsp;Xiaohong Li ,&nbsp;Peng Zhang ,&nbsp;Minghao Xu ,&nbsp;Jitong Zhao ,&nbsp;Jingjing Yan ,&nbsp;Chenli Qiu ,&nbsp;Jiayi Shu ,&nbsp;Shuo Zhang ,&nbsp;Miaomiao Kang ,&nbsp;Xiaoyan Zhang ,&nbsp;Jianqing Xu ,&nbsp;Shuye Zhang","doi":"10.1016/j.virs.2024.03.004","DOIUrl":"10.1016/j.virs.2024.03.004","url":null,"abstract":"<div><p>The utilization of enteroviruses engineered with reporter genes serves as a valuable tool for advancing our understanding of enterovirus biology and its applications, enabling the development of effective therapeutic and preventive strategies. In this study, our initial attempts to introduce a NanoLuc luciferase (NLuc) reporter gene into recombinant enteroviruses were unsuccessful in rescuing viable progenies. We hypothesized that the size of the inserted tag might be a determining factor in the rescue of the virus. Therefore, we inserted the 11-amino-acid HiBiT tag into the genomes of enterovirus A71 (EV-A71), coxsackievirus A10 (CVA10), coxsackievirus A7 (CVA7), coxsackievirus A16 (CVA16), namely EV-A71-HiBiT, CVA16-HiBiT, CVA10-HiBiT, CVA7-HiBiT, and observed that the HiBiT-tagged viruses exhibited remarkably high rescue efficiency. Notably, the HiBiT-tagged enteroviruses displayed comparable characteristics to the wild-type viruses. A direct comparison between CVA16-NLuc and CVA16-HiBiT recombinant viruses revealed that the tiny HiBiT insertion had minimal impact on virus infectivity and replication kinetics. Moreover, these HiBiT-tagged enteroviruses demonstrated high genetic stability in different cell lines over multiple passages. In addition, the HiBiT-tagged viruses were successfully tested in antiviral drug assays, and the sensitivity of the viruses to drugs was not affected by the HiBiT tag. Ultimately, our findings provide definitive evidence that the integration of HiBiT into enteroviruses presents a universal, convenient, and invaluable method for advancing research in the realm of enterovirus virology. Furthermore, HiBiT-tagged enteroviruses exhibit great potential for diverse applications, including the development of antivirals and the elucidation of viral infection mechanisms.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 422-433"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000300/pdfft?md5=684258996e73d4fe09675c8af02c1934&pid=1-s2.0-S1995820X24000300-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140159131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calmodulin-like 5 promotes PEDV replication by regulating late-endosome synthesis and innate immune response","authors":"Wen-Jun Tian ,&nbsp;Xiu-Zhong Zhang ,&nbsp;Jing Wang ,&nbsp;Jian-Feng Liu ,&nbsp;Fu-Huang Li ,&nbsp;Xiao-Jia Wang","doi":"10.1016/j.virs.2024.05.006","DOIUrl":"10.1016/j.virs.2024.05.006","url":null,"abstract":"<div><p>The infection caused by porcine epidemic diarrhea virus (PEDV) is associated with high mortality in piglets worldwide. Host factors involved in the efficient replication of PEDV, however, remain largely unknown. Our recent proteomic study in the virus-host interaction network revealed a significant increase in the accumulation of CALML5 (EF-hand protein calmodulin-like 5) following PEDV infection. A further study unveiled a biphasic increase of CALML5 in 2 and 12 ​h after viral infection. Similar trends were observed in the intestines of piglets in the early and late stages of the PEDV challenge. Moreover, CALML5 depletion reduced PEDV mRNA and protein levels, leading to a one-order-of-magnitude decrease in virus titer. At the early stage of PEDV infection, CALML5 affected the endosomal trafficking pathway by regulating the expression of endosomal sorting complex related cellular proteins. CALML5 depletion also suppressed IFN-β and IL-6 production in the PEDV-infected cells, thereby indicating its involvement in negatively regulating the innate immune response. Our study reveals the biological function of CALML5 in the virology field and offers new insights into the PEDV-host cell interaction.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 501-512"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000750/pdfft?md5=d989749110750ad0e5fce2621f50b1da&pid=1-s2.0-S1995820X24000750-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141094124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}