Virologica Sinica最新文献_第10页

Circulation patterns and molecular characteristics of respiratory syncytial virus among hospitalized children in Tianjin, China, before and during the COVID-19 pandemic (2017–2022) COVID-19 流行前和流行期间（2017-2022 年）中国天津住院儿童呼吸道合胞病毒的流行模式和分子特征。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.07.004

Mengzhu Hou , Guangping Liu , Chao Meng , Lili Dong , Yulian Fang , Lu Wang , Ning Wang , Chunquan Cai , Hanjie Wang

{"title":"Circulation patterns and molecular characteristics of respiratory syncytial virus among hospitalized children in Tianjin, China, before and during the COVID-19 pandemic (2017–2022)","authors":"Mengzhu Hou , Guangping Liu , Chao Meng , Lili Dong , Yulian Fang , Lu Wang , Ning Wang , Chunquan Cai , Hanjie Wang","doi":"10.1016/j.virs.2024.07.004","DOIUrl":"10.1016/j.virs.2024.07.004","url":null,"abstract":"<div><div>Respiratory syncytial virus (RSV) is the main pathogen that causes hospitalization for acute lower respiratory tract infections (ALRIs) in children. With the reopening of communities and schools, the resurgence of RSV in the COVID-19 post-pandemic era has become a major concern. To understand the circulation patterns and genotype variability of RSV in Tianjin before and during the COVID-19 pandemic, a total of 19,531 nasopharyngeal aspirate samples from hospitalized children in Tianjin from July 2017 to June 2022 were evaluated. Direct immunofluorescence and polymerase chain reaction (PCR) were used for screening RSV-positive samples and subtyping, respectively. Further analysis of mutations in the second hypervariable region (HVR2) of the <em>G</em> gene was performed through Sanger sequencing. Our results showed that 16.46% (3215/19,531) samples were RSV positive and a delayed increase in the RSV infection rates occurred in the winter season from December 2020 to February 2021, with the average RSV-positive rate of 35.77% (519/1451). The ON1, with H258Q and H266L substitutions, and the BA9, with T290I and T312I substitutions, are dominant strains that alternately circulate every 1–2 years in Tianjin, China, from July 2017 to June 2022. In addition, novel substitutions, such as N296Y, K221T, N230K, V251A in the BA9 genotype, and L226I in the ON1 genotype, emerged during the COVID-19 pandemic. Analysis of clinical characteristics indicated no significant differences between RSV-A and RSV-B groups. This study provides a theoretical basis for clinical prevention and treatment. However, further studies are needed to explore the regulatory mechanism of host immune responses to different lineages of ON1 and BA9 in the future.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 5","pages":"Pages 719-726"},"PeriodicalIF":5.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analyses and antigenic characterization of foot-and-mouth disease virus PanAsia lineage circulating in China between 1999 and 2023 1999年至2023年期间在中国流行的口蹄疫病毒泛亚系的系统发育分析和抗原特征。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.09.006

Xiangle Zhang , Weimin Ma , Baohong Liu , Chaochao Shen , Fan Yang , Yamin Yang , Lv Lv , Jinyan Wu , Yongjie Liu , Youjun Shang , Jianhong Guo , Zixiang Zhu , Xiangtao Liu , Haixue Zheng , Jijun He

{"title":"Phylogenetic analyses and antigenic characterization of foot-and-mouth disease virus PanAsia lineage circulating in China between 1999 and 2023","authors":"Xiangle Zhang , Weimin Ma , Baohong Liu , Chaochao Shen , Fan Yang , Yamin Yang , Lv Lv , Jinyan Wu , Yongjie Liu , Youjun Shang , Jianhong Guo , Zixiang Zhu , Xiangtao Liu , Haixue Zheng , Jijun He","doi":"10.1016/j.virs.2024.09.006","DOIUrl":"10.1016/j.virs.2024.09.006","url":null,"abstract":"<div><div>Foot-and-mouth disease (FMD) is one of the most important transboundary animal diseases caused by foot-and-mouth disease virus (FMDV), leading to significant economic losses worldwide. The first report of PanAsia lineage of FMDV in China was in 1999. Since 2011, 18 outbreaks attributed to PanAsia lineage viruses have been reported across 7 provinces or municipality in China. Phylogenetic analysis indicated that these PanAsia strains were clustered into three distinct clades (clade 1, clade 2, and clade 3), with nucleotide homology ranging from 91.4% to 100%. The outbreaks of FMD caused by clade 1 strains occurred around 1999 when this lineage was prevalent globally. Clade 2 strains dominated from 2011 to 2013, while clade 3 strains were prevalent during 2018–2019, sharing only 93% homology with clade 2 strains and 91% with clade 1 strains. Tracing analysis showed that these outbreaks represented 3 distinct introductions of PanAsia viruses into China. Virus neutralization tests (VNT) have demonstrated that current commercial vaccines are effective to protect susceptible animals against these strains (<em>r1</em> > 0.3). However, the growing demand for livestock has promoted animal movement and encouraged the exchange of products, services, and materials between countries, thereby heightening the risk of exotic strain incursions. Therefore, it is imperative to reinforce border controls and limit animal movements among various Asian countries continually to reduce the risk of new transboundary diseases, such as FMD incursion. Additionally, PanAsia-2 strains need to be taken seriously to prevent its incursions, and the relevant vaccines against PanAsia-2 strains need to be stockpiled in preparation for any possible incursion.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 5","pages":"Pages 747-754"},"PeriodicalIF":5.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142257246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virome-wide analysis of histone modification mimicry motifs carried by viral proteins 对病毒蛋白所携带的组蛋白修饰模拟图案进行全病毒组分析。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.09.004

Yang Xiao , Shuofeng Yuan , Ye Qiu , Xing-Yi Ge

{"title":"Virome-wide analysis of histone modification mimicry motifs carried by viral proteins","authors":"Yang Xiao , Shuofeng Yuan , Ye Qiu , Xing-Yi Ge","doi":"10.1016/j.virs.2024.09.004","DOIUrl":"10.1016/j.virs.2024.09.004","url":null,"abstract":"<div><div>Histone mimicry (HM) refers to the presence of short linear motifs in viral proteins that mimic critical regions of host histone proteins. These motifs have the potential to interfere with host cell epigenome and counteract antiviral response. Recent research shows that HM is critical for the pathogenesis and transmissibility of influenza virus and coronavirus. However, the distribution, characteristics, and functions of HM in eukaryotic viruses remain obscure. Herein, we developed a bioinformatic pipeline, Histone Motif Scan (HiScan), to identify HM motifs in viral proteins and predict their functions <em>in silico</em>. By analyzing 592,643 viral proteins using HiScan, we found that putative HM motifs were widely distributed in most viral proteins. Among animal viruses, the ratio of HM motifs between DNA viruses and RNA viruses was approximately 1.9:1, and viruses with smaller genomes had a higher density of HM motifs. Notably, coronaviruses exhibited an uneven distribution of HM motifs, with betacoronaviruses (including most human pathogenic coronaviruses) harboring more HM motifs than other coronaviruses, primarily in the NSP3, S, and N proteins. In summary, our virome-wide screening of HM motifs using HiScan revealed extensive but uneven distribution of HM motifs in most viral proteins, with a preference in DNA viruses. Viral HM may play an important role in modulating viral pathogenicity and virus-host interactions, making it an attractive area of research in virology and antiviral medication.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 5","pages":"Pages 793-801"},"PeriodicalIF":5.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142257548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid diagnosis of a fox's death case using nanopore sequencing reveals the infection with an Artic-like rabies virus 利用纳米孔测序技术对狐狸死亡病例进行快速诊断，发现感染了一种类似北极的狂犬病毒。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.08.010

Yuhang Liu , Zhiqiang Liu , Jian Li , Xiaomin Yan , Weidi Xu , Le Yi , Changchun Tu , Biao He

引用次数: 0

A novel vesicular stomatitis virus armed with IL-2 mimic for oncolytic therapy 用于溶瘤疗法的新型水泡性口炎病毒与 IL-2 模拟物。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.09.007

Manman Wu , Yiwei Wang , Chuanjian Wu , Huang Huang , Xinyuan Zhou , Jun Wang , Sidong Xiong , Chunsheng Dong

{"title":"A novel vesicular stomatitis virus armed with IL-2 mimic for oncolytic therapy","authors":"Manman Wu , Yiwei Wang , Chuanjian Wu , Huang Huang , Xinyuan Zhou , Jun Wang , Sidong Xiong , Chunsheng Dong","doi":"10.1016/j.virs.2024.09.007","DOIUrl":"10.1016/j.virs.2024.09.007","url":null,"abstract":"<div><div>Oncolytic virus (OV) is increasingly being recognized as a novel vector in cancer immunotherapy. Increasing evidence suggests that OV has the ability to change the immune status of tumor microenvironment, so called transformation of ‘cold’ tumors into ‘hot’ tumors. The improved anti-tumor immunity can be induced by OV and further enhanced through the combination of various immunomodulators. The Neo-2/15 is a newly de novo synthesized cytokine that functions as both IL-2 and IL-15. However, it specifically lacks the binding site of IL-2 receptor α subunit (CD25), therefore unable to induce the Treg proliferation. In present study, a recombinant vesicular stomatitis virus expressing the Neo-2/15 (VSV<sup>M51R</sup>-Neo-2/15) was generated. Intratumoral delivery of VSV<sup>M51R</sup>-Neo-2/15 efficiently inhibited tumor growth in mice without causing the IL-2-related toxicity previously observed in clinic. Moreover, treatment with VSV<sup>M51R</sup>-Neo-2/15 increased the number of activated CD8<sup>+</sup> T cells but not Treg cells in tumors. More tumor-bearing mice were survival with VSV<sup>M51R</sup>-Neo-2/15 treatment, and the surviving mice displayed enhanced protection against tumor cell rechallenge due to the induced anti-tumor immunity. In addition, combination therapy of OV and anti-PD-L1 immune checkpoint inhibitors further enhanced the anti-tumor immune response. These findings suggest that our novel VSV<sup>M51R</sup>-Neo-2/15 can effectively inhibit the tumor growth and enhance the sensitivity to immune checkpoint inhibitors, providing promising attempts for further clinical trials.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 5","pages":"Pages 821-832"},"PeriodicalIF":5.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a novel caspase cleavage motif AEAD 鉴定新型 Caspase 裂解基序 AEAD。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.08.001

Yujie Fang , Zhou Gong , Miaomiao You , Ke Peng

{"title":"Identification of a novel caspase cleavage motif AEAD","authors":"Yujie Fang , Zhou Gong , Miaomiao You , Ke Peng","doi":"10.1016/j.virs.2024.08.001","DOIUrl":"10.1016/j.virs.2024.08.001","url":null,"abstract":"<div><div>Infections of many viruses induce caspase activation to regulate multiple cellular pathways, including programmed cell death, immune signaling and etc. Characterizations of caspase cleavage sites and substrates are important for understanding the regulation mechanisms of caspase activation. Here, we identified and analyzed a novel caspase cleavage motif AEAD, and confirmed its caspase dependent cleavage activity in natural substrate, such as nitric oxide-associated protein 1 (NOA1). Fusing the enhanced green fluorescent protein (EGFP) with the mitochondrial marker protein Tom20 through the AEAD motif peptide localized EGFP to the mitochondria. Upon the activation of caspase triggered by Sendai virus (SeV) or herpes simplex virus type 1 (HSV-1) infection, EGFP diffusely localized to the cell due to the caspase-mediated cleavage, thus allowing visual detection of the virus-induced caspase activation. An AEAD peptide-derived inhibitor Z-AEAD-FMK were developed, which significantly inhibited the activities of caspases-1, -3, -6, -7, -8 and -9, exhibiting a broad caspase inhibition effect. The inhibitor further prevented caspases-mediated cleavage of downstream substrates, including BID, PARP1, LMNA, pro-IL-1β, pro-IL-18, GSDMD and GSDME, protecting cells from virus-induced apoptotic and pyroptotic cell death. Together, our findings provide a new perspective for the identification of novel caspase cleavage motifs and the development of new caspase inhibitors and anti-inflammatory drugs.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 5","pages":"Pages 755-766"},"PeriodicalIF":5.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141890204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The spatiotemporal analysis of SARS-CoV-2 transmission in China since the termination of the dynamic zero-COVID policy 自动态零COVID政策终止以来中国SARS-CoV-2传播的时空分析。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.09.003

Jiaying Li , Jingqi Yang , Xiao Ding , Hangyu Zhou , Na Han , Aiping Wu

{"title":"The spatiotemporal analysis of SARS-CoV-2 transmission in China since the termination of the dynamic zero-COVID policy","authors":"Jiaying Li , Jingqi Yang , Xiao Ding , Hangyu Zhou , Na Han , Aiping Wu","doi":"10.1016/j.virs.2024.09.003","DOIUrl":"10.1016/j.virs.2024.09.003","url":null,"abstract":"<div><div>China's dynamic zero-COVID policy has effectively curbed the spread of SARS-CoV-2, while inadvertently creating immunity gaps within its population. Subsequent surges in COVID-19 cases linked to various SARS-CoV-2 lineages post-policy termination necessitate a thorough investigation into the epidemiological landscape. This study addresses this issue by analyzing a comprehensive dataset of 39,456 high-quality genomes collected nationwide over an 11-month period since policy termination. Through lineage assignment, phylogenetic analysis, pandemic pattern comparison, phylodynamic reconstruction, and recombination detection, we found that China's post-epidemic period could be divided into three stages, along with dynamic changes in dominant lineages. Geographical clustering of similar lineages implies the importance of cross-border cooperation among neighboring regions. Compared to the USA, UK, and Japan, China exhibits unique trajectories of lineage epidemics, characterized by initial lagging followed by subsequent advancement, indicating the potential influence of diverse prevention and control policies on lineage epidemic patterns. Hong Kong, Shanghai, and Hubei emerge as pivotal nodes in the nationwide spread, marking a shift in the transmission center from east to central regions of China. Although China hasn't experienced significant variant emergence, the detection and validation of the novel recombination event, XCN lineage, underscore the ongoing virus evolution. Overall, this study systematically analyzes the spatiotemporal transmission of SARS-CoV-2 virus in China since the termination of the dynamic zero-COVID policy, offering valuable insights for regional surveillance and evidence-based public health policymaking.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 5","pages":"Pages 737-746"},"PeriodicalIF":5.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142257549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The unique immune evasion mechanisms of the mpox virus and their implication for developing new vaccines and immunotherapies Mpox 病毒独特的免疫规避机制及其对开发新型疫苗和免疫疗法的影响。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.08.008

Dong Fang , Yan Liu , Dou Dou , Bin Su

{"title":"The unique immune evasion mechanisms of the mpox virus and their implication for developing new vaccines and immunotherapies","authors":"Dong Fang , Yan Liu , Dou Dou , Bin Su","doi":"10.1016/j.virs.2024.08.008","DOIUrl":"10.1016/j.virs.2024.08.008","url":null,"abstract":"<div><div>Mpox is an infectious and contagious zoonotic disease caused by the mpox virus (MPXV), which belongs to the genus <em>Orthopoxvirus</em>. Since 2022, MPXV has posed a significant threat to global public health. The emergence of thousands of cases across the Western Hemisphere prompted the World Health Organization to declare an emergency. The extensive coevolutionary history of poxviruses with humans has enabled these viruses to develop sophisticated mechanisms to counter the human immune system. Specifically, MPXV employs unique immune evasion strategies against a wide range of immunological elements, presenting a considerable challenge for treatment, especially following the discontinuation of routine smallpox vaccination among the general population. In this review, we start by discussing the entry of the mpox virus and the onset of early infection, followed by an introduction to the mechanisms by which the mpox virus can evade the innate and adaptive immune responses. Two caspase-1 inhibitory proteins and a PKR escape-related protein have been identified as phylogenomic hubs involved in modulating the immune environment during the MPXV infection. With respect to adaptive immunity, mpox viruses exhibit unique and exceptional T-cell inhibition capabilities, thereby comprehensively remodeling the host immune environment. The viral envelope also poses challenges for the neutralizing effects of antibodies and the complement system. The unique immune evasion mechanisms employed by MPXV make novel multi-epitope and nucleic acid-based vaccines highly promising research directions worth investigating. Finally, we briefly discuss the impact of MPXV infection on immunosuppressed patients and the current status of MPXV vaccine development. This review may provide valuable information for the development of new immunological treatments for mpox.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 5","pages":"Pages 709-718"},"PeriodicalIF":5.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trivalent SARS-CoV-2 virus-like particle vaccines exhibit broad-spectrum neutralization and protection against XBB.1 and BA.2.86 variants 三价 SARS-CoV-2 病毒样颗粒疫苗对 XBB.1 和 BA.2.86 变体具有广谱中和和保护作用。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.08.005

Lu Zhang , Siyu Tian , Jun Dai , Yuanyuan Li , Yu Zhou , Yan Li , Jiao Xu , Shuyun Liu , Zhiwei Lin , Zhaoyong Zhang , Jiantao Chen , Peilan Wei , Jingxian Zhao , Jing Jin , Yanqun Wang , Jincun Zhao

引用次数: 0

Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo 维生素 D 衍生物在体外和体内对严重发热伴血小板减少综合征病毒的抗病毒活性。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-10-01 DOI: 10.1016/j.virs.2024.08.007

Chongda Luo , Xintong Yan , Shaokang Yang , Sichen Ren , Yan Luo , Jiazheng Li , Ping Wang , Yunfeng Shao , Wei Li , Song Li , Jingjing Yang , Ruiyuan Cao , Wu Zhong

{"title":"Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo","authors":"Chongda Luo , Xintong Yan , Shaokang Yang , Sichen Ren , Yan Luo , Jiazheng Li , Ping Wang , Yunfeng Shao , Wei Li , Song Li , Jingjing Yang , Ruiyuan Cao , Wu Zhong","doi":"10.1016/j.virs.2024.08.007","DOIUrl":"10.1016/j.virs.2024.08.007","url":null,"abstract":"<div><div>Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne virus that causes the severe fever thrombocytopenia syndrome, which manifests as fever and haemorrhage, accompanied by severe neurological complications. To date, no specific antiviral drugs have been approved for this indication. Herein, we investigated whether vitamin D derivatives inhibit SFTSV both <em>in vitro</em> and <em>in vivo</em>. An <em>in vitro</em> study demonstrated that vitamin D derivatives significantly suppressed viral RNA replication, plaque formation, and protein expression in a dose-dependent manner. Subsequently, <em>in vivo</em> studies revealed that doxercalciferol and alfacalcidol were associated with increased survival and reduced viral RNA load in the blood. Time-of-addition assay suggested that vitamin D derivatives primarily acted during the post-entry phase of SFTSV infection. However, cytopathic effect protective activity was not observed in RIG-I immunodeficient cell line Huh7.5, and the administration of vitamin D derivatives did not improve the survival rates or reduce the blood viral loads in adult A129 mice. Further transcriptome exploration into the antiviral mechanism revealed that alfacalcidol stimulates host innate immunity to exert antiviral effects. To expand the application of vitamin D derivatives, <em>in vitro</em> and <em>in vivo</em> drug combination assays were performed, which highlighted the synergistic effects of vitamin D derivatives and T-705 on SFTSV. The combination of alfacalcidol and T-705 significantly enhanced the therapeutic effects in mice. This study highlights the potential of vitamin D derivatives against SFTSV and suggests that they may have synergistic effects with other compounds used in the treatment of SFTSV infection.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 5","pages":"Pages 802-811"},"PeriodicalIF":5.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0