Virologica Sinica最新文献_第9页

Insights into recent advancements in human and animal rotavirus vaccines: Exploring new frontiers 人类和动物轮状病毒疫苗的最新进展：探索新领域。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.001

Ahmed H. Ghonaim , Sherin R. Rouby , Wedad M. Nageeb , Ashraf Ahmed Elgendy , Rong Xu , Changsheng Jiang , Noha H. Ghonaim , Qigai He , Wentao Li

引用次数: 0

Distinct tropisms of HCMV and SARS-CoV-2 in lung tissue of a patient with advanced HIV disease 晚期HIV患者肺组织中HCMV和SARS-CoV-2的明显趋向性

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2025.01.001

Jingjing Xu , Bo Yang , Ye Zheng , Yuexiang Yang , Min-Hua Luo , Yun Ling , Xiaohong Fan , Han Cheng

引用次数: 0

The potential mechanisms and material basis of Fuzheng Jiedu decoction broad-spectrum inhibiting coronaviruses 扶正解毒汤广谱抑制冠状病毒的潜在机制和物质基础。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.007

Ke Liu , Bixia Hong , Shi-Ting He , Siying Du , Jiayi Ke , Lili Tian , Tao Tao , Yihan Zhang , Kelin Li , Han Chang , Mengzhe Li , Xiaoping An , Lihua Song , Zhongde Zhang , Liang Liu , Hudan Pan , Huahao Fan , Yigang Tong

{"title":"The potential mechanisms and material basis of Fuzheng Jiedu decoction broad-spectrum inhibiting coronaviruses","authors":"Ke Liu , Bixia Hong , Shi-Ting He , Siying Du , Jiayi Ke , Lili Tian , Tao Tao , Yihan Zhang , Kelin Li , Han Chang , Mengzhe Li , Xiaoping An , Lihua Song , Zhongde Zhang , Liang Liu , Hudan Pan , Huahao Fan , Yigang Tong","doi":"10.1016/j.virs.2024.12.007","DOIUrl":"10.1016/j.virs.2024.12.007","url":null,"abstract":"<div><div>Traditional Chinese medicine has unique advantages in preventing and treating COVID-19, and Fuzheng Jiedu decoction (FZJDD) was reported to be effective against COVID-19 in clinical trials. To investigate the potential mechanisms and material basis of FZJDD against SARS-CoV-2, we performed SARS-CoV-2 target protein inhibition analyses and a metabolite full spectrum analysis of FZJDD. Interestingly, FZJDD was found to block the binding of SARS-CoV-2 Spike protein with the receptor ACE2 and inhibit the activity of SARS-CoV-2 3CLpro. Moreover, FZJDD can regulate the TNF and the MAPK signaling pathway to inhibit the inflammatory response and alleviate the “cytokine storm”. A total of 298 compounds were identified in FZJDD, among them, caffeic acid and octyl gallate were found to be the potential therapeutic agents of FZJDD. Importantly, FZJDD can broadly inhibit coronavirus infection, including SADS-CoV and porcine epidemic diarrhea virus (PEDV) live viruses, SARS-CoV, MERS-CoV, and SARS-CoV-2 mutant pseudotyped viruses, which might be ascribed to the broad-spectrum anti-coronavirus activity of caffeic acid and octyl gallate. In conclusion, this study reveals the mechanisms and material basis of FZJDD against SARS-CoV-2 and identifies the broad-spectrum anti-coronavirus activity of FZJDD for the first time. Our data provide empirical evidence for the development and application of FZJDD.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 125-136"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A low pathogenic avian influenza A/Mallard/South Korea/KNU2019-34/2019 (H1N1) virus has the potential to increase the mammalian pathogenicity 低致病性禽流感A/野鸭/韩国/KNU2019-34/2019 （H1N1）病毒具有提高哺乳动物致病性的潜力。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.005

Jaemoo Kim , Jungho Kim , Suhyeon Heo , Chang-Hun Yeom , Bao Tuan Duong , Haan Woo Sung , Seon-Ju Yeo , Hyun Park , Haryoung Poo , Jihyun Yang

{"title":"A low pathogenic avian influenza A/Mallard/South Korea/KNU2019-34/2019 (H1N1) virus has the potential to increase the mammalian pathogenicity","authors":"Jaemoo Kim , Jungho Kim , Suhyeon Heo , Chang-Hun Yeom , Bao Tuan Duong , Haan Woo Sung , Seon-Ju Yeo , Hyun Park , Haryoung Poo , Jihyun Yang","doi":"10.1016/j.virs.2024.12.005","DOIUrl":"10.1016/j.virs.2024.12.005","url":null,"abstract":"<div><div>Influenza, a highly contagious respiratory infectious disease caused by an influenza virus, is a threat to public health worldwide. Avian influenza viruses (AIVs) have the potential to cause the next pandemic by crossing the species barrier through mutation of viral genome. Here, we investigated the pathogenicity of AIVs obtained from South Korea and Mongolia during 2018–2019 by measuring viral titers in the lungs and extrapulmonary organs of mouse models. In addition, we assessed the pathogenicity of AIVs in ferret models. Moreover, we compared the ability of viruses to replicate in mammalian cells, as well as the receptor-binding preferences of AIV isolates. Genetic analyses were finally performed to identify the genetic relationships and amino acid substitutions between viral proteins during mammalian adaptation. Of the 24 AIV isolates tested, A/Mallard/South Korea/KNU2019-34/2019 (KNU19-34; H1N1) caused severe bodyweight loss and high mortality in mice. The virus replicated in the lungs, kidneys, and heart. Importantly, KNU19-34-infected ferrets showed high viral loads in both nasal washes and lungs. KNU19-34 replicated rapidly in A549 and bound preferentially to human like α2,6-linked sialic acids rather than to avian-like α2,3-linked sialic acids, similar to the pandemic A/California/04/2009 (H1N1) strain. Gene segments of KNU19-34 were distributed in Egypt and Asia lineages from 2015 to 2018, and the virus had several amino acid substitutions compared to H1N1 AIV isolates that were non-pathogenic in mice. Collectively, the data suggest that KNU19-34 has zoonotic potential and the possibility of new mutations responsible for mammalian adaptation.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 24-34"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PB2 627V and HA 217 sites synergistically affect the lethality of H9N2 in mice PB2 627V 和 HA 217 位点协同影响 H9N2 对小鼠的致死率。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.003

Lingcai Zhao, Miao Tian, Xifeng Hu, Menglu Fan, Chenglin Hou, Jihui Ping

{"title":"PB2 627V and HA 217 sites synergistically affect the lethality of H9N2 in mice","authors":"Lingcai Zhao, Miao Tian, Xifeng Hu, Menglu Fan, Chenglin Hou, Jihui Ping","doi":"10.1016/j.virs.2024.12.003","DOIUrl":"10.1016/j.virs.2024.12.003","url":null,"abstract":"<div><div>The H9N2 subtype avian influenza virus (AIV) continues to propagate and undergo evolution within China, thereby posing a significant threat to the poultry industry. This study encompassed the collection of 436 samples and swabs in East China over the period spanning 2018 to 2019, from which 31 strains of the H9N2 subtype viruses were isolated and purified. We revealed that the <em>HA</em> and <em>NA</em> genes of the 31 isolates categorized within the Y280 branch, while the <em>PB2</em> and <em>M</em> genes were associated with the G1 branch, and the remaining genes aligned with the F/98 branch. Despite this alignment, antigenic mapping demonstrated differences between the 2018 and 2019 strains, with the early vaccine strains displaying low serological reactivity toward these isolates. Notably, the CK/SH/49/19 isolate exhibited lethality in mice, characterized by a PB2 E627V mutation and a HA deletion at amino acid position 217. Mechanistically, <em>in vitro</em> studies showed that the influenza virus CK/SH/49/19 carrying PB2 627V and HA 217M mutations displayed enhanced replication capacity, attributed to the heightened activity of the polymerase with PB2 627V. Moreover, the absence of the amino acid at the HA 217 site obstructed viral adsorption and internalization, resulted in lower activation pH, and impeded the virus budding process. Critically, <em>in vivo</em> experiments revealed that CK/SH/49/19 (PB2 627V, HA 217Δ) triggered a robust activation of interferon response and interferon-stimulated genes. This study furnished a theoretical foundation for the scientific prevention and control strategies against H9N2 subtype avian influenza.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 35-49"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zika virus infections and associated risk factors among pregnant women in Gombe, Nigeria 尼日利亚贡贝孕妇的寨卡病毒感染及其相关风险因素。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.008

Oderinde Soji Bamidele , Abdulbasi Bakoji , Samaila Jackson Yaga , Kunle Ijaya , Bukar Mohammed , Ismaila Yunusa Yuguda , Marycelin M. Baba

{"title":"Zika virus infections and associated risk factors among pregnant women in Gombe, Nigeria","authors":"Oderinde Soji Bamidele , Abdulbasi Bakoji , Samaila Jackson Yaga , Kunle Ijaya , Bukar Mohammed , Ismaila Yunusa Yuguda , Marycelin M. Baba","doi":"10.1016/j.virs.2024.12.008","DOIUrl":"10.1016/j.virs.2024.12.008","url":null,"abstract":"<div><div>In-utero exposure to Zika virus (ZIKV) could lead to miscarriage, preterm birth and congenital Zika syndrome. This study aimed at estimating the burden of ZIKV and Dengue virus (DENV) infections among pregnant women in Bojude, Nigeria. A total of 200 blood samples were collected from pregnant women between February and April 2022. Using the updated CDC guidelines for the diagnosis of ZIKV infections, including ELISA and microneutralization test (MNT), we found that 16.5% of participants were positive for ZIKV IgM, 10% were positive for IgG, and 23% had nAb in their serum. Among the 46 ZIKV nAb-positive women, 52.2% and 10.9% were recent and previous ZIKV infections, respectively, while 6.5% had previous DENV infections. Although no recent DENV infection was detected, recent and previous ZIKV/DENV co-infections were 13.0% and 17.4%, respectively. Two participants had recent secondary ZIKV infections, while 39.1% had prolonged lifelong immunity. Recent ZIKV infection rates were significantly higher among sexually active females aged 20–29 years than other age groups, with the highest risk observed in the first trimester of pregnancy. In addition, the grand-multiparous women are at higher risk of ZIKV infections than other categories. Monotypic recent, secondary and past ZIKV infections, as well as DENV and ZIKV co-infections, were detected in both the asymptomatic and symptomatic pregnant women. These findings highlight that ZIKV infection is prevalent among pregnant women in Nigeria and underscore the associated risk factors, providing evidence-based information on the burden of ZIKV infections in DENV-endemic region.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 61-70"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis between genotypes of adenovirus isolates from hospitalized children with acute respiratory tract infections and clinical manifestations in Wuhan, China, from June 2022 to September 2023 2022年6月- 2023年9月武汉市急性呼吸道感染住院患儿腺病毒分离株基因型与临床表现的比较分析

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.004

Chunchen Wu , Yanfang Zhang , Ao Liang , Xiaoxue Wu , Yaqi Zhu , Zhaoxuan Huang , Jun Wang , Yali Deng , Lixian Pan , Anbang Wang , Fei Deng , Jianbo Xia

{"title":"Comparative analysis between genotypes of adenovirus isolates from hospitalized children with acute respiratory tract infections and clinical manifestations in Wuhan, China, from June 2022 to September 2023","authors":"Chunchen Wu , Yanfang Zhang , Ao Liang , Xiaoxue Wu , Yaqi Zhu , Zhaoxuan Huang , Jun Wang , Yali Deng , Lixian Pan , Anbang Wang , Fei Deng , Jianbo Xia","doi":"10.1016/j.virs.2024.12.004","DOIUrl":"10.1016/j.virs.2024.12.004","url":null,"abstract":"<div><div>Acute respiratory tract infections (ARTIs) are among the leading causes of morbidity and mortality in children worldwide. Human adenovirus (HAdV) infections are estimated to account for at least 5% of pediatric ARTIs. The circulated genotypes of HAdV and the correlation between genotype and clinical manifestations in Wuhan, China, before and after the complete relaxation of nonpharmaceutical interventions against severe acute respiratory syndrome coronavirus 2, remain unknown. Here, 101 HAdV strains were isolated from throat swab samples collected from hospitalized children with ARTIs who tested positive for HAdV nucleic acid. Of these, sixty-six strains from 2022 to twenty-three strains from 2023 were successfully genotyped and subjected to phylogenetic analysis based on the hexon, penton base, and fiber genes. Six genotypes, B3, C1, C2, C5, C104, and C108 were identified. HAdV-B3 (84.85%) was the most prevalent type in 2022, while HAdV-C (86.96%), including C1, C2, C108, and C104, was the most prevalent in 2023. These strains were phylogenetically related to strains from Japan, China, and the United States in recent years. When comparing clinical characteristics, pediatric patients infected with B3, C1, C2, C5, C104, or C108 exhibited similar clinical manifestations, primarily fever and cough, but varying interleukin (IL)-10 levels. In conclusion, from June 2022 to September 2023, the circulated genotypes of HAdV in Wuhan included B3, C1, C2, C108, C5, and C104. The endemic pattern of HAdV in Wuhan, China, shifted from species B as the dominant type in 2022 to species C in 2023.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 50-60"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRTC3 restricts SARS-CoV-2 replication and is antagonized by CREB CRTC3限制SARS-CoV-2复制并被CREB拮抗。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.006

Li Yang , Xiao-Tao Zeng , Rong-Hua Luo , Ying Tang , Si-Xue Ren , Xin-Yan Long , Xiang-Hui Fu , Wan-Jiang Zhang , Hai-Yan Ren , Yong-Tang Zheng , Wei Cheng

{"title":"CRTC3 restricts SARS-CoV-2 replication and is antagonized by CREB","authors":"Li Yang , Xiao-Tao Zeng , Rong-Hua Luo , Ying Tang , Si-Xue Ren , Xin-Yan Long , Xiang-Hui Fu , Wan-Jiang Zhang , Hai-Yan Ren , Yong-Tang Zheng , Wei Cheng","doi":"10.1016/j.virs.2024.12.006","DOIUrl":"10.1016/j.virs.2024.12.006","url":null,"abstract":"<div><div>Virus-encoding RNA-dependent RNA polymerase (RdRp) is essential for genome replication and gene transcription of human coronaviruses (HCoVs), including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We previously identified the interaction between the catalytic subunit NSP12 of SARS-CoV-2 RdRp and the host protein CREB-regulated transcription coactivator 3 (CRTC3), a member of the CRTC family that regulates cyclic AMP response element-binding protein (CREB)-mediated transcriptional activation. Currently, the implication of CRTC3 in the pathogenesis of HCoVs is poorly understood. Herein, we demonstrated that CRTC3 attenuates RdRp activity and SARS-CoV-2 genome replication, therefore reducing the production of progeny viruses. The interaction of CRTC3 with NSP12 contributes to its inhibitory effect on RdRp activity. Furthermore, we expanded the suppressive effects of two other CRTC family members (CRTC1 and CRTC2) on the RdRp activities of lethal HCoVs, including SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV), along with the CREB antagonization. Overall, our research suggests that CRTCs restrict the replication of HCoVs and are antagonized by CREB, which not only provides new insights into the replication regulation of HCoVs, but also offers important information for the development of anti-HCoV interventions.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 92-108"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Random forest algorithm reveals novel sites in HA protein that shift receptor binding preference of the H9N2 avian influenza virus 随机森林算法揭示了HA蛋白中改变H9N2禽流感病毒受体结合偏好的新位点。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.12.010

Yuncong Yin , Wen Li , Rujian Chen , Xiao Wang , Yiting Chen , Xinyuan Cui , Xingbang Lu , David M. Irwin , Xuejuan Shen , Yongyi Shen

{"title":"Random forest algorithm reveals novel sites in HA protein that shift receptor binding preference of the H9N2 avian influenza virus","authors":"Yuncong Yin , Wen Li , Rujian Chen , Xiao Wang , Yiting Chen , Xinyuan Cui , Xingbang Lu , David M. Irwin , Xuejuan Shen , Yongyi Shen","doi":"10.1016/j.virs.2024.12.010","DOIUrl":"10.1016/j.virs.2024.12.010","url":null,"abstract":"<div><div>A switch from avian-type α-2,3 to human-type α-2,6 receptors is an essential element for the initiation of a pandemic from an avian influenza virus. Some H9N2 viruses exhibit a preference for binding to human-type α-2,6 receptors. This identifies their potential threat to public health. However, our understanding of the molecular basis for the switch of receptor preference is still limited. In this study, we employed the random forest algorithm to identify the potentially key amino acid sites within hemagglutinin (HA), which are associated with the receptor binding ability of H9N2 avian influenza virus (AIV). Subsequently, these sites were further verified by receptor binding assays. A total of 12 substitutions in the HA protein (N158D, N158S, A160 N, A160D, A160T, T163I, T163V, V190T, V190A, D193 N, D193G, and N231D) were predicted to prefer binding to α-2,6 receptors. Except for the V190T substitution, the other substitutions were demonstrated to display an affinity for preferential binding to α-2,6 receptors by receptor binding assays. Especially, the A160T substitution caused a significant upregulation of immune-response genes and an increased mortality rate in mice. Our findings provide novel insights into understanding the genetic basis of receptor preference of the H9N2 AIV.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 109-117"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigenic analysis of the influenza B virus hemagglutinin protein 乙型流感病毒血凝素蛋白的抗原分析。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-02-01 DOI: 10.1016/j.virs.2024.08.012

Mengyi Zhang , Chaoying Yang , Xi Wu , Yifei Wang , Lijie Wang , Qianqian Cui , Jincheng Tong , Yimeng An , Meina Cai , Shishi Cheng , Qi Jiang , Yulin Wang , Chenyan Zhao , Youchun Wang , Weijin Huang

{"title":"Antigenic analysis of the influenza B virus hemagglutinin protein","authors":"Mengyi Zhang , Chaoying Yang , Xi Wu , Yifei Wang , Lijie Wang , Qianqian Cui , Jincheng Tong , Yimeng An , Meina Cai , Shishi Cheng , Qi Jiang , Yulin Wang , Chenyan Zhao , Youchun Wang , Weijin Huang","doi":"10.1016/j.virs.2024.08.012","DOIUrl":"10.1016/j.virs.2024.08.012","url":null,"abstract":"<div><div>Influenza B viruses (IBVs) primarily infect humans and are a common cause of respiratory infections in humans. Here, to systematically analyze the antigenicity of the IBVs Hemagglutinin (HA) protein, 31 B/Victoria and 19 B/Yamagata representative circulating strains were selected from Global Initiative of Sharing All Influenza Data (GISAID), and pseudotyped viruses were constructed with the vesicular stomatitis virus system. Guinea pigs were immunized with three doses of vaccines (one dose of DNA vaccines following two doses of pseudotyped virus vaccines) of the seven IBV vaccine strains, and neutralizing antibodies against the pseudotyped viruses were tested. By comparing differences between various vaccine strains, we constructed several pseudotyped viruses that contained various mutations based on vaccine strain BV-21. The vaccine strains showed good neutralization levels against the epidemic virus strains of the same year, with neutralization titers ranging from 370 to 840, while the level of neutralization against viruses prevalent in previous years decreased 1–10-fold. Each of the high-frequency epidemic strains of B/Victoria and B/Yamagata not only induced high neutralizing titers, but also had broadly neutralizing effects against virus strains of different years, with neutralizing titers ranging from 1000 to 7200. R141G, D197 N, and R203K were identified as affecting the antigenicity of IBV. These mutation sites provide valuable references for the selection and design of a universal IBV vaccine strain in the future.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 1","pages":"Pages 80-91"},"PeriodicalIF":5.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0