Virologica Sinica最新文献

{"title":"Detection of a reassortant swine H1N2 influenza A virus from pigs in Hong Kong","authors":"Guoqian Gu , Congnuan Liu , Song Hao Lee , Lewis Sze Chun Choi , Michael T. Wilson , Dirk U. Pfeiffer , Yun Young Go","doi":"10.1016/j.virs.2024.01.009","DOIUrl":"10.1016/j.virs.2024.01.009","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 343-346"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000099/pdfft?md5=36e9e0675e7cf3c2ae2cf9cacb510ac7&pid=1-s2.0-S1995820X24000099-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139664500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRRSV degrades MDA5 via dual autophagy receptors P62 and CCT2 to evade antiviral innate immunity","authors":"Ruiqi Sun ,&nbsp;Yanyu Guo ,&nbsp;Lilin Zhang ,&nbsp;Huixia Zhang,&nbsp;Boxuan Yin,&nbsp;Xiaoyang Li,&nbsp;Changyan Li,&nbsp;Liu Yang,&nbsp;Lei Zhang,&nbsp;Zexing Li,&nbsp;Jinhai Huang","doi":"10.1016/j.virs.2024.01.005","DOIUrl":"10.1016/j.virs.2024.01.005","url":null,"abstract":"<div><p>Porcine reproductive and respiratory syndrome virus (PRRSV) is a major economically devastating pathogen that has evolved various strategies to evade innate immunity. Downregulation of antiviral interferon largely promotes PRRSV immunoevasion by utilizing cytoplasmic melanoma differentiation-associated gene 5 (MDA5), a receptor that senses viral RNA. In this study, the downregulated transcription and expression levels of porcine MDA5 in PRRSV infection were observed, and the detailed mechanisms were explored. We found that the interaction between P62 and MDA5 is enhanced due to two factors: the phosphorylation modification of the autophagic receptor P62 by the upregulated kinase CK2α and the K63 ubiquitination of porcine MDA5 catalyzed by the E3 ubiquitinase TRIM21 in PRRSV-infected cells. As a result of these modifications, the classic P62-mediated autophagy is triggered. Additionally, porcine MDA5 interacts with the chaperonin containing TCP1 subunit 2 (CCT2), which is enhanced by PRRSV nsp3. This interaction promotes the aggregate formation and autophagic clearance of MDA5-CCT2-nsp3 independently of ubiquitination. In summary, enhanced MDA5 degradation occurs in PRRSV infection via two autophagic pathways: the binding of MDA5 with the autophagy receptor P62 and the aggrephagy receptor CCT2, leading to intense innate immune suppression. The research reveals a novel mechanism of immune evasion in PRRSV infection and provides fundamental insights for the development of new vaccines or therapeutic strategies.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 264-276"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000051/pdfft?md5=0b3858b10b3467a6c1d2887e4c0a8d69&pid=1-s2.0-S1995820X24000051-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139557491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PABP-driven secondary condensed phase within RSV inclusion bodies activates viral mRNAs for ribosomal recruitment","authors":"Qiang Zhang ,&nbsp;Hanzhe Ye ,&nbsp;Cong Liu ,&nbsp;Haiwu Zhou ,&nbsp;Mingbin He ,&nbsp;Xiaodong Liang ,&nbsp;Yu Zhou ,&nbsp;Kun Wang ,&nbsp;Yali Qin ,&nbsp;Zhifei Li ,&nbsp;Mingzhou Chen","doi":"10.1016/j.virs.2023.12.001","DOIUrl":"10.1016/j.virs.2023.12.001","url":null,"abstract":"<div><p>Inclusion bodies (IBs) of respiratory syncytial virus (RSV) are formed by liquid-liquid phase separation (LLPS) and contain internal structures termed “IB-associated granules” (IBAGs), where anti-termination factor M2-1 and viral mRNAs are concentrated. However, the mechanism of IBAG formation and the physiological function of IBAGs are unclear. Here, we found that the internal structures of RSV IBs are actual M2-1-free viral messenger ribonucleoprotein (mRNP) condensates formed by secondary LLPS. Mechanistically, the RSV nucleoprotein (N) and M2-1 interact with and recruit PABP to IBs, promoting PABP to bind viral mRNAs transcribed in IBs by RNA-recognition motif and drive secondary phase separation. Furthermore, PABP-eIF4G1 interaction regulates viral mRNP condensate composition, thereby recruiting specific translation initiation factors (eIF4G1, eIF4E, eIF4A, eIF4B and eIF4H) into the secondary condensed phase to activate viral mRNAs for ribosomal recruitment. Our study proposes a novel LLPS-regulated translation mechanism during viral infection and a novel antiviral strategy via targeting on secondary condensed phase.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 235-250"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X23001438/pdfft?md5=a107f65c4a02e2b6c73994a206fd6b5d&pid=1-s2.0-S1995820X23001438-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138566953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association analysis of genetic variants in critical patients with COVID-19 and validation in a Chinese population","authors":"Yi Yin ,&nbsp;Yi Zhang ,&nbsp;Lelin Sun ,&nbsp;Shuqiang Wang ,&nbsp;Yong Zeng ,&nbsp;Bo Gong ,&nbsp;Lulin Huang ,&nbsp;Yongquan He ,&nbsp;Zhenglin Yang","doi":"10.1016/j.virs.2024.02.004","DOIUrl":"10.1016/j.virs.2024.02.004","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 347-350"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000245/pdfft?md5=0f92e6ae9b1398748336342d1924bdd2&pid=1-s2.0-S1995820X24000245-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes","authors":"Ru-Yi Chen ,&nbsp;Teng Zhao ,&nbsp;Jing-Jing Guo ,&nbsp;Feng Zhu ,&nbsp;Na-Na Zhang ,&nbsp;Xiao-Feng Li ,&nbsp;Hai-Tao Liu ,&nbsp;Fei Wang ,&nbsp;Yong-Qiang Deng ,&nbsp;Cheng-Feng Qin","doi":"10.1016/j.virs.2024.03.002","DOIUrl":"10.1016/j.virs.2024.03.002","url":null,"abstract":"<div><p>Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from <em>Culex</em> spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 ​cells. Further <em>in vitro</em> characterization revealed that the two GCXVs exhibited comparable replication kinetics, protein expression and viral titers. Importantly, GCXV RNAs were detected in the bodies, salivary glands, midguts and ovaries of <em>Culex quinquefasciatus</em> at 4–10 days after oral infection. In addition, two GCXVs can colonize <em>Cx. quinquefasciatus</em> eggs, resulting in positive rates of 15%–35% for the second gonotrophic cycle. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in <em>Cx. quinquefasciatus</em> eggs during the first and second gonotrophic cycles after oral infection.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 228-234"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000282/pdfft?md5=3cbbfb045f21e9f2800c3710ab9cd042&pid=1-s2.0-S1995820X24000282-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coxsackievirus B3 HFMD animal models in Syrian hamster and rhesus monkey","authors":"Suqin Duan ,&nbsp;Wei Zhang ,&nbsp;Yongjie Li,&nbsp;Yanyan Li,&nbsp;Yuan Zhao,&nbsp;Weihua Jin,&nbsp;Quan Liu,&nbsp;Mingxue Li,&nbsp;Wenting Sun,&nbsp;Lixiong Chen,&nbsp;Hongjie Xu,&nbsp;Jie Tang,&nbsp;Jinghan Hou,&nbsp;Zijun Deng,&nbsp;Fengmei Yang,&nbsp;Shaohui Ma,&nbsp;Zhanlong He","doi":"10.1016/j.virs.2024.02.001","DOIUrl":"10.1016/j.virs.2024.02.001","url":null,"abstract":"<div><p>Coxsackievirus B3 (CVB3) is the pathogen causing hand, foot and mouth disease (HFMD), which manifests across a spectrum of clinical severity from mild to severe. However, CVB3-infected mouse models mainly demonstrate viral myocarditis and pancreatitis, failing to replicate human HFMD symptoms. Although several enteroviruses have been evaluated in Syrian hamsters and rhesus monkeys, there is no comprehensive data on CVB3. In this study, we have first tested the susceptibility of Syrian hamsters to CVB3 infection via different routes. The results showed that Syrian hamsters were successfully infected with CVB3 by intraperitoneal injection or nasal drip, leading to nasopharyngeal colonization, acute severe pathological injury, and typical HFMD symptoms. Notably, the nasal drip group exhibited a longer viral excretion cycle and more severe pathological damage. In the subsequent study, rhesus monkeys infected with CVB3 through nasal drips also presented signs of HFMD symptoms, viral excretion, serum antibody conversion, viral nucleic acids and antigens, and the specific organ damages, particularly in the heart. Surprisingly, there were no significant differences in myocardial enzyme levels, and the clinical symptoms resembled those often associated with common, mild infections. In summary, the study successfully developed severe Syrian hamsters and mild rhesus monkey models for CVB3-induced HFMD. These models could serve as a basis for understanding the disease pathogenesis, conducting pre-trial prevention and evaluation, and implementing post-exposure intervention.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 290-300"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000117/pdfft?md5=6ab46977ef63ec5efc1e7f4619fdb9c8&pid=1-s2.0-S1995820X24000117-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139708057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viromes of Haemaphysalis longicornis reveal different viral abundance and diversity in free and engorged ticks","authors":"Jian Xiao ,&nbsp;Xuan Yao ,&nbsp;Xuhua Guan ,&nbsp;Jinfeng Xiong ,&nbsp;Yaohui Fang ,&nbsp;Jingyuan Zhang ,&nbsp;You Zhang ,&nbsp;Abulimiti Moming ,&nbsp;Zhengyuan Su ,&nbsp;Jiayin Jin ,&nbsp;Yingying Ge ,&nbsp;Jun Wang ,&nbsp;Zhaojun Fan ,&nbsp;Shuang Tang ,&nbsp;Shu Shen ,&nbsp;Fei Deng","doi":"10.1016/j.virs.2024.02.003","DOIUrl":"10.1016/j.virs.2024.02.003","url":null,"abstract":"<div><p><em>Haemaphysalis longicornis</em> ticks, commonly found in East Asia, can transmit various pathogenic viruses, including the severe fever with thrombocytopenia syndrome virus (SFTSV) that has caused febrile diseases among humans in Hubei Province. However, understanding of the viromes of <em>H. longicornis</em> was limited, and the prevalence of viruses among <em>H. longicornis</em> ticks in Hubei was not well clarified. This study investigates the viromes of both engorged (fed) and free (unfed) <em>H. longicornis</em> ticks across three mountainous regions in Hubei Province from 2019 to 2020. RNA-sequencing analysis identified viral sequences that were related to 39 reference viruses belonging to unclassified viruses and seven RNA viral families, namely <em>Chuviridae</em>, <em>Nairoviridae</em>, <em>Orthomyxoviridae</em>, <em>Parvoviridae</em>, <em>Phenuiviridae</em>, <em>Rhabdoviridae</em>, and <em>Totiviridae.</em> Viral abundance and diversity in these ticks were analysed, and phylogenetic characteristics of the Henan tick virus (HNTV), Dabieshan tick virus (DBSTV), Okutama tick virus (OKTV), and Jingmen tick virus (JMTV) were elucidated based on their full genomic sequences. Prevalence analysis demonstrated that DBSTV was the most common virus found in individual <em>H. longicornis</em> ticks (12.59%), followed by HNTV (0.35%), whereas JMTV and OKTV were not detected. These results improve our understanding of <em>H. longicornis</em> tick viromes in central China and highlight the role of tick feeding status and geography in shaping the viral community. The findings of new viral strains and their potential impact on public health raise the need to strengthen surveillance efforts for comprehensively assessing their spillover potentials.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 194-204"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000233/pdfft?md5=5198021da7cac8232664e75a3c8f831d&pid=1-s2.0-S1995820X24000233-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139742188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mast cell degranulation-triggered by SARS-CoV-2 induces tracheal-bronchial epithelial inflammation and injury","authors":"Jian-Bo Cao ,&nbsp;Shu-Tong Zhu ,&nbsp;Xiao-Shan Huang ,&nbsp;Xing-Yuan Wang ,&nbsp;Meng-Li Wu ,&nbsp;Xin Li ,&nbsp;Feng-Liang Liu ,&nbsp;Ling Chen ,&nbsp;Yong-Tang Zheng ,&nbsp;Jian-Hua Wang","doi":"10.1016/j.virs.2024.03.001","DOIUrl":"10.1016/j.virs.2024.03.001","url":null,"abstract":"<div><p>SARS-CoV-2 infection-induced hyper-inflammation is a key pathogenic factor of COVID-19. Our research, along with others', has demonstrated that mast cells (MCs) play a vital role in the initiation of hyper-inflammation caused by SARS-CoV-2. In previous study, we observed that SARS-CoV-2 infection induced the accumulation of MCs in the peri-bronchus and bronchioalveolar-duct junction in humanized mice. Additionally, we found that MC degranulation triggered by the spike protein resulted in inflammation in alveolar epithelial cells and capillary endothelial cells, leading to subsequent lung injury. The trachea and bronchus are the routes for SARS-CoV-2 transmission after virus inhalation, and inflammation in these regions could promote viral spread. MCs are widely distributed throughout the respiratory tract. Thus, in this study, we investigated the role of MCs and their degranulation in the development of inflammation in tracheal-bronchial epithelium. Histological analyses showed the accumulation and degranulation of MCs in the peri-trachea of humanized mice infected with SARS-CoV-2. MC degranulation caused lesions in trachea, and the formation of papillary hyperplasia was observed. Through transcriptome analysis in bronchial epithelial cells, we found that MC degranulation significantly altered multiple cellular signaling, particularly, leading to upregulated immune responses and inflammation. The administration of ebastine or loratadine effectively suppressed the induction of inflammatory factors in bronchial epithelial cells and alleviated tracheal injury in mice. Taken together, our findings confirm the essential role of MC degranulation in SARS-CoV-2-induced hyper-inflammation and the subsequent tissue lesions. Furthermore, our results support the use of ebastine or loratadine to inhibit SARS-CoV-2-triggered degranulation, thereby preventing tissue damage caused by hyper-inflammation.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 309-318"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000270/pdfft?md5=e0311093800cc3c27e1cef340f052ff6&pid=1-s2.0-S1995820X24000270-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140065935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C1QTNF5 is a novel attachment factor that facilitates the entry of influenza A virus","authors":"Lei Yu ,&nbsp;Xinjin Liu ,&nbsp;Xiaoqin Wei ,&nbsp;Junrui Ren ,&nbsp;Xueyun Wang ,&nbsp;Shuwen Wu ,&nbsp;Ke Lan","doi":"10.1016/j.virs.2024.01.003","DOIUrl":"10.1016/j.virs.2024.01.003","url":null,"abstract":"<div><p>Influenza A virus (IAV) binds sialic acid receptors on the cell surface to enter the host cells, which is the key step in initiating infection, transmission and pathogenesis. Understanding the factors that contribute to the highly efficient entry of IAV into human cells will help elucidate the mechanism of viral entry and pathogenicity, and provide new targets for intervention. In the present study, we reported a novel membrane protein, C1QTNF5, which binds to the hemagglutinin protein of IAV and promotes IAV infection <em>in vitro</em> and <em>in vivo</em>. We found that the HA1 region of IAV hemagglutinin is critical for the interaction with C1QTNF5 protein, and C1QTNF5 interacts with hemagglutinin mainly through its N-terminus (1–103 aa). In addition, we further demonstrated that overexpression of C1QTNF5 promotes IAV entry, while blocking the interaction between C1QTNF5 and IAV hemagglutinin greatly inhibits viral entry. However, C1QTNF5 does not function as a receptor to mediate IAV infection in sialic acid-deficient CHO-Lec2 cells, but promotes IAV to attach to these cells, suggesting that C1QTNF5 is an important attachment factor for IAV. This work reveals C1QTNF5 as a novel IAV attachment factor and provides a new perspective for antiviral strategies.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 277-289"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000038/pdfft?md5=45c720d5a7ea8aa121c5587416233433&pid=1-s2.0-S1995820X24000038-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139508203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural analysis of conformational changes in the mpox virus A7 protein","authors":"Xincheng Ni,&nbsp;Kai Wang,&nbsp;Yinze Han,&nbsp;Jian Lei","doi":"10.1016/j.virs.2023.12.006","DOIUrl":"10.1016/j.virs.2023.12.006","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 331-334"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X2300158X/pdfft?md5=9f74211b4d81a4cdb32a3eb1025c33e3&pid=1-s2.0-S1995820X2300158X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139071835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}