Virologica Sinica最新文献

{"title":"Crucial role played by CK8+ cells in mediating alveolar injury remodeling for patients with COVID-19","authors":"Yufeng Li ,&nbsp;Hengrui Hu ,&nbsp;Jia Liu ,&nbsp;Longda Ma ,&nbsp;Xi Wang ,&nbsp;Liang Liu ,&nbsp;Qian Liu ,&nbsp;Liang Ren ,&nbsp;Jiang Li ,&nbsp;Fei Deng ,&nbsp;Zhihong Hu ,&nbsp;Yiwu Zhou ,&nbsp;Manli Wang","doi":"10.1016/j.virs.2024.03.007","DOIUrl":"10.1016/j.virs.2024.03.007","url":null,"abstract":"<div><p>The high risk of SARS-CoV-2 infection and reinfection and the occurrence of post-acute pulmonary sequelae have highlighted the importance of understanding the mechanism underlying lung repair after injury. To address this concern, comparative and systematic analyses of SARS-CoV-2 infection in COVID-19 patients and animals were conducted. In the lungs of nine patients who died of COVID-19 and one recovered from COVID-19 but died of unrelated disease in early 2020, damage-related transient progenitor (DATP) cells expressing CK8 marker proliferated significantly. These CK8⁺ DATP cells were derived from bronchial CK5⁺ basal cells. However, they showed different cell fate toward differentiation into type I alveolar cells in the deceased and convalescent patients, respectively. By using a self-limiting hamster infection model mimicking the dynamic process of lung injury remodeling in mild COVID-19 patients, the accumulation and regression of CK8⁺ cell marker were found to be closely associated with the disease course. Finally, we examined the autopsied lungs of two patients who died of infection by the recent Omicron variant and found that they only exhibited mild pathological injury with no CK8⁺ cell proliferation. These results indicate a clear pulmonary cell remodeling route and suggest that CK8⁺ DATP cells play a primary role in mediating alveolar remodeling, highlighting their potential applications as diagnostic markers and therapeutic targets.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 390-402"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000336/pdfft?md5=22d53f65357c9873217455d5a9e74c27&pid=1-s2.0-S1995820X24000336-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140194686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe fever with thrombocytopenia syndrome virus infection attributed to cat contact: A case report in Beijing, China","authors":"Shuo Zhang ,&nbsp;Yu Leng ,&nbsp;Fa-Xue Zhang ,&nbsp;Bo-Kang Fu ,&nbsp;Bing-Zheng Wu ,&nbsp;Jia-Hao Chen ,&nbsp;Ning Cui ,&nbsp;Ning Zhao ,&nbsp;Wei Liu ,&nbsp;Hao Li","doi":"10.1016/j.virs.2024.03.006","DOIUrl":"10.1016/j.virs.2024.03.006","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 513-515"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000324/pdfft?md5=4b645003568a2442cee38f83164d0a54&pid=1-s2.0-S1995820X24000324-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140185669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Joint deletion of multifunctional MGF505-7R and H240R genes generates a safe and effective African swine fever virus attenuated live vaccine candidate","authors":"Changjiang Weng ,&nbsp;Li Huang ,&nbsp;Guangqiang Ye","doi":"10.1016/j.virs.2024.04.007","DOIUrl":"10.1016/j.virs.2024.04.007","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 355-357"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000671/pdfft?md5=ac43f14191886c091cef7a79d0db3f10&pid=1-s2.0-S1995820X24000671-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140872122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobodies against African swine fever virus p72 and CD2v proteins as reagents for developing two cELISAs to detect viral antibodies","authors":"Jiahong Zhu ,&nbsp;Qingyuan Liu ,&nbsp;Liuya Li,&nbsp;Runyu Zhang,&nbsp;Yueting Chang,&nbsp;Jiakai Zhao,&nbsp;Siyu Liu,&nbsp;Xinyu Zhao,&nbsp;Xu Chen,&nbsp;Yani Sun,&nbsp;Qin Zhao","doi":"10.1016/j.virs.2024.04.002","DOIUrl":"10.1016/j.virs.2024.04.002","url":null,"abstract":"<div><p>African swine fever virus (ASFV) poses a significant threat to the global swine industry. Currently, there are no effective vaccines or treatments available to combat ASFV infection in pigs. The primary means of controlling the spread of the disease is through rapid detection and subsequent elimination of infected pig. Recently, a lower virulent ASFV isolate with a deleted <em>EP402R</em> gene (CD2v-deleted) has been reported in China, which further complicates the control of ASFV infection in pig farms. Furthermore, an <em>EP402R</em>-deleted ASFV variant has been developed as a potential live attenuated vaccine candidate strain. Therefore, it is crucial to develop detection methods that can distinguish wild-type and <em>EP402R</em>-deleted ASFV infections. In this study, two recombinant ASFV-p72 and -CD2v proteins were expressed using a prokaryotic system and used to immunize Bactrian camels. Subsequently, eight nanobodies against ASFV-p72 and ten nanobodies against ASFV-CD2v were screened. Following the production of these nanobodies with horse radish peroxidase (HRP) fusion proteins, the ASFV-p72-Nb2-HRP and ASFV-CD2v-Nb22-HRP fusions were selected for the development of two competitive ELISAs (cELISAs) to detect anti-ASFV antibodies. The two cELISAs exhibited high sensitivity, good specificity, repeatability, and stability. The coincidence rate between the two cELISAs and commercial ELISA kits was 98.6% and 97.6%, respectively. Collectively, the two cELISA for detecting antibodies against ASFV demonstrated ease of operation, a low cost, and a simple production process. The two cELISAs could determine whether pigs were infected with wild-type or CD2v-deleted ASFV, and could play an important role in monitoring ASFV infections in pig farms.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 3","pages":"Pages 478-489"},"PeriodicalIF":5.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000385/pdfft?md5=5a037a55939982ad032019f43dc7906a&pid=1-s2.0-S1995820X24000385-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140772672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ten computational challenges in human virome studies.","authors":"Yifan Wu, Yousong Peng","doi":"10.1016/j.virs.2024.04.008","DOIUrl":"10.1016/j.virs.2024.04.008","url":null,"abstract":"<p><p>In recent years, substantial advancements have been achieved in understanding the diversity of the human virome and its intricate roles in human health and diseases. Despite this progress, the field of human virome research remains nascent, primarily hindered by the lack of effective methods, particularly in the domain of computational tools. This perspective systematically outlines ten computational challenges spanning various types of virome studies. These challenges arise due to the vast diversity of viromes, the absence of a universal marker gene in viral genomes, the low abundance of virus populations, the remote or minimal homology of viral proteins to known proteins, and the highly dynamic and heterogeneous nature of viromes. For each computational challenge, we discuss the underlying reasons, current research progress, and potential solutions. The resolution of these challenges necessitates ongoing collaboration among computational scientists, virologists, and multidisciplinary experts. In essence, this perspective serves as a comprehensive guide for directing computational efforts in human virome studies.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"草乌甲素对哮喘小鼠治疗作用及其机制研究","authors":"宋乔文 | 许月野 | 郭伟 | 湛孝东 | 赵金红 | 李媛媛 | 徐静云 | 唐小牛","doi":"10.13350/j.cjpb.240412","DOIUrl":"https://doi.org/10.13350/j.cjpb.240412","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"1 1","pages":""},"PeriodicalIF":5.5,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142033282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative and qualitative subgenomic RNA profiles of SARS-CoV-2 in respiratory samples: A comparison between Omicron BA.2 and non-VOC-D614G","authors":"Zigui Chen ,&nbsp;Rita Way Yin Ng ,&nbsp;Grace Lui ,&nbsp;Lowell Ling ,&nbsp;Agnes S.Y. Leung ,&nbsp;Chit Chow ,&nbsp;Siaw Shi Boon ,&nbsp;Wendy C.S. Ho ,&nbsp;Maggie Haitian Wang ,&nbsp;Renee Wan Yi Chan ,&nbsp;Albert Martin Li ,&nbsp;David Shu Cheong Hui ,&nbsp;Paul Kay Sheung Chan","doi":"10.1016/j.virs.2024.01.010","DOIUrl":"10.1016/j.virs.2024.01.010","url":null,"abstract":"<div><p>The SARS-CoV-2 Omicron variants are notorious for their transmissibility, but little is known about their subgenomic RNA (sgRNA) expression. This study applied RNA-seq to delineate the quantitative and qualitative profiles of canonical sgRNA of 118 respiratory samples collected from patients infected with Omicron BA.2 and compared with 338 patients infected with non-variant of concern (non-VOC)-D614G. A unique characteristic profile depicted by the relative abundance of 9 canonical sgRNAs was reproduced by both BA.2 and non-VOC-D614G regardless of host gender, age and presence of pneumonia. Remarkably, such profile was lost in samples with low viral load, suggesting a potential application of sgRNA pattern to indicate viral activity of individual patient at a specific time point. A characteristic qualitative profile of canonical sgRNAs was also reproduced by both BA.2 and non-VOC-D614G. The presence of a full set of canonical sgRNAs carried a coherent correlation with crude viral load (AUC ​= ​0.91, 95% CI 0.88–0.94), and sgRNA ORF7b was identified to be the best surrogate marker allowing feasible routine application in characterizing the infection status of individual patient. Further potentials in using sgRNA as a target for vaccine and antiviral development are worth pursuing.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 218-227"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000105/pdfft?md5=b05b93c660d9a44723b9d711c55462a9&pid=1-s2.0-S1995820X24000105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139679814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution, pathogenicity and transmissibility of quadruple reassortant H1N2 swine influenza virus in China: A potential threat to public health","authors":"Xinxin Cui ,&nbsp;Jinhuan Ma ,&nbsp;Zifeng Pang ,&nbsp;Lingzhi Chi ,&nbsp;Cuishan Mai ,&nbsp;Hanlin Liu ,&nbsp;Ming Liao ,&nbsp;Hailiang Sun","doi":"10.1016/j.virs.2024.02.002","DOIUrl":"10.1016/j.virs.2024.02.002","url":null,"abstract":"<div><p>Swine are regarded as “intermediate hosts” or “mixing vessels” of influenza viruses, capable of generating strains with pandemic potential. From 2020 to 2021, we conducted surveillance on swine H1N2 influenza (swH1N2) viruses in swine farms located in Guangdong, Yunnan, and Guizhou provinces in southern China, as well as Henan and Shandong provinces in northern China. We systematically analyzed the evolution and pathogenicity of swH1N2 isolates, and characterized their replication and transmission abilities. The isolated viruses are quadruple reassortant H1N2 viruses containing genes from pdm/09 H1N1 (<em>PB2</em>, <em>PB1</em>, <em>PA</em> and <em>NP</em> genes), triple-reassortant swine (<em>NS</em> gene), Eurasian Avian-like (<em>HA</em> and <em>M</em> genes), and recent human H3N2 (<em>NA</em> gene) lineages. The <em>NA</em>, <em>PB2</em>, and <em>NP</em> of SW/188/20 and SW/198/20 show high gene similarities to A/Guangdong/Yue Fang277/2017 (H3N2). The <em>HA</em> gene of swH1N2 exhibits a high evolutionary rate. The five swH1N2 isolates replicate efficiently in human, canine, and swine cells, as well as in the turbinate, trachea, and lungs of mice. A/swine/Shandong/198/2020 strain efficiently replicates in the respiratory tract of pigs and effectively transmitted among them. Collectively, these current swH1N2 viruses possess zoonotic potential, highlighting the need for strengthened surveillance of swH1N2 viruses.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 205-217"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000221/pdfft?md5=d22179fa690fe0b61ae0dcaf2d61daf2&pid=1-s2.0-S1995820X24000221-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139724143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of fangchinoline as a broad-spectrum enterovirus inhibitor through reporter virus based high-content screening","authors":"Qiu-Yan Zhang ,&nbsp;Jia-Qi Li ,&nbsp;Qi Li ,&nbsp;Yang Zhang ,&nbsp;Zhe-Rui Zhang ,&nbsp;Xiao-Dan Li ,&nbsp;Hong-Qing Zhang ,&nbsp;Cheng-Lin Deng ,&nbsp;Feng-Xia Yang ,&nbsp;Yi Xu ,&nbsp;Bo Zhang","doi":"10.1016/j.virs.2024.02.006","DOIUrl":"10.1016/j.virs.2024.02.006","url":null,"abstract":"<div><p>Hand, foot, and mouth disease (HFMD) is a common pediatric illness mainly caused by enteroviruses, which are important human pathogens. Currently, there are no available antiviral agents for the therapy of enterovirus infection. In this study, an excellent high-content antiviral screening system utilizing the EV-A71-eGFP reporter virus was developed. Using this screening system, we screened a drug library containing 1042 natural compounds to identify potential EV-A71 inhibitors. Fangchinoline (FAN), a bis-benzylisoquinoline alkaloid, exhibits potential inhibitory effects against various enteroviruses that cause HFMD, such as EV-A71, CV-A10, CV-B3 and CV-A16. Further investigations revealed that FAN targets the early stage of the enterovirus life cycle. Through the selection of FAN-resistant EV-A71 viruses, we demonstrated that the VP1 protein could be a potential target of FAN, as two mutations in VP1 (E145G and V258I) resulted in viral resistance to FAN. Our research suggests that FAN is an efficient inhibitor of EV-A71 and has the potential to be a broad-spectrum antiviral drug against human enteroviruses.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 301-308"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000269/pdfft?md5=d5620464f2cf2dd4c93981bcef5dc1d9&pid=1-s2.0-S1995820X24000269-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140054589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-proteolytic ubiquitination of HBx controls HBV replication","authors":"Xiangpeng Sheng ,&nbsp;Yi Yang ,&nbsp;Min Zhu ,&nbsp;Linlin Zhou ,&nbsp;Fang Zhu ,&nbsp;Yuanfei Zhu ,&nbsp;Siying Dong ,&nbsp;Hui Kong ,&nbsp;Honghua Wang ,&nbsp;Ji Jiang ,&nbsp;Mingyue Wan ,&nbsp;Mingyang Feng ,&nbsp;Qiang Deng ,&nbsp;Yumin Xu ,&nbsp;Qing You ,&nbsp;Ronggui Hu","doi":"10.1016/j.virs.2024.01.008","DOIUrl":"10.1016/j.virs.2024.01.008","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 2","pages":"Pages 338-342"},"PeriodicalIF":5.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000087/pdfft?md5=6ca6e32762e89a9a0074551a7332ee1c&pid=1-s2.0-S1995820X24000087-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139664434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}