Virologica Sinica最新文献_第8页

Transmission restriction and genomic evolution co-shape the genetic diversity patterns of influenza A virus 传播限制和基因组进化共同塑造了甲型流感病毒的遗传多样性模式。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.02.005

{"title":"Transmission restriction and genomic evolution co-shape the genetic diversity patterns of influenza A virus","authors":"","doi":"10.1016/j.virs.2024.02.005","DOIUrl":"10.1016/j.virs.2024.02.005","url":null,"abstract":"<div><p>Influenza A virus (IAV) shows an extensive host range and rapid genomic variations, leading to continuous emergence of novel viruses with significant antigenic variations and the potential for cross-species transmission. This causes global pandemics and seasonal flu outbreaks, posing sustained threats worldwide. Thus, studying all IAVs' evolutionary patterns and underlying mechanisms is crucial for effective prevention and control. We developed FluTyping to identify IAV genotypes, to explore overall genetic diversity patterns and their restriction factors. FluTyping groups isolates based on genetic distance and phylogenetic relationships using whole genomes, enabling identification of each isolate's genotype. Three distinct genetic diversity patterns were observed: one genotype domination pattern comprising only H1N1 and H3N2 seasonal influenza subtypes, multi-genotypes co-circulation pattern including majority avian influenza subtypes and swine influenza H1N2, and hybrid-circulation pattern involving H7N9 and three H5 subtypes of influenza viruses. Furthermore, the IAVs in multi-genotypes co-circulation pattern showed region-specific dominant genotypes, implying the restriction of virus transmission is a key factor contributing to distinct genetic diversity patterns, and the genomic evolution underlying different patterns was more influenced by host-specific factors. In summary, a comprehensive picture of the evolutionary patterns of overall IAVs is provided by the FluTyping's identified genotypes, offering important theoretical foundations for future prevention and control of these viruses.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 525-536"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000257/pdfft?md5=c9047b76f99283ea406765a10aa07add&pid=1-s2.0-S1995820X24000257-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139997623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Individual virome analysis reveals the general co-infection of mammal-associated viruses with SARS-related coronaviruses in bats 单个病毒组分析表明，哺乳动物相关病毒与蝙蝠体内与严重急性呼吸系统综合症有关的冠状病毒普遍混合感染。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.06.008

{"title":"Individual virome analysis reveals the general co-infection of mammal-associated viruses with SARS-related coronaviruses in bats","authors":"","doi":"10.1016/j.virs.2024.06.008","DOIUrl":"10.1016/j.virs.2024.06.008","url":null,"abstract":"<div><p>Bats are the natural reservoir hosts for SARS-related coronavirus (SARSr-CoV) and other highly pathogenic microorganisms. Therefore, it is conceivable that an individual bat may harbor multiple microbes. However, there is limited knowledge on the overall co-circulation of microorganisms in bats. Here, we conducted a 16-year monitoring of bat viruses in south and central China and identified 238 SARSr-CoV positive samples across nine bat species from ten provinces or administrative districts. Among these, 76 individual samples were selected for further metagenomics analysis. We found a complex microenvironment characterized by the general co-circulation of microbes from two different sources: mammal-associated viruses or environment-associated microbes. The later includes commensal bacteria, enterobacteria-related phages, and insect or fungal viruses of food origin. Results showed that 25% (19/76) of the samples contained at least one another mammal-associated virus, notably alphacoronaviruses (13/76) such as AlphaCoV/YN2012, HKU2-related CoV and AlphaCoV/Rf-HuB2013, along with viruses from other families. Notably, we observed three viruses co-circulating within a single bat, comprising two coronavirus species and one picornavirus. Our analysis also revealed the potential presence of pathogenic bacteria or fungi in bats. Furthermore, we obtained 25 viral genomes from the 76 bat SARSr-CoV positive samples, some of which formed new evolutionary lineages. Collectively, our study reveals the complex microenvironment of bat microbiome, facilitating deeper investigations into their pathogenic potential and the likelihood of cross-species transmission.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 565-573"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X2400107X/pdfft?md5=95d5d8994d71b59e3d52f410e01cb7a3&pid=1-s2.0-S1995820X2400107X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continued evolution of H10N3 influenza virus with adaptive mutations poses an increased threat to mammals 具有适应性突变的 H10N3 流感病毒不断进化，对哺乳动物的威胁日益严重。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.06.005

{"title":"Continued evolution of H10N3 influenza virus with adaptive mutations poses an increased threat to mammals","authors":"","doi":"10.1016/j.virs.2024.06.005","DOIUrl":"10.1016/j.virs.2024.06.005","url":null,"abstract":"<div><p>The H10 subtype avian influenza virus (AIV) poses an ongoing threat to both birds and humans. Notably, fatal human cases of H10N3 and H10N8 infections have drawn public attention. In 2022, we isolated two H10N3 viruses (A/chicken/Shandong/0101/2022 and A/chicken/Shandong/0603/2022) from diseased chickens in China. Genome analysis revealed that these viruses were genetically associated with human-origin H10N3 virus, with internal genes originating from local H9N2 viruses. Compared to the H10N8 virus (A/chicken/Jiangxi/102/2013), the H10N3 viruses exhibited enhanced thermostability, increased viral release from erythrocytes, and accumulation of hemagglutinin (HA) protein. Additionally, we evaluated the pathogenicity of both H10N3 and H10N8 viruses in mice. We found that viral titers could be detected in the lungs and nasal turbinates of mice infected with the two H10N3 viruses, whereas H10N8 virus titers were detectable in the lungs and brains of mice. Notably, the proportion of double HA Q222R and G228S mutations in H10N3 viruses has increased since 2019. However, the functional roles of the Q222R and G228S double mutations in the <em>HA</em> gene of H10N3 viruses remain unknown and warrant further investigation. Our study highlights the potential public health risk posed by the H10N3 virus. A spillover event of AIV to humans could be a foretaste of a looming pandemic. Therefore, it is imperative to continuously monitor the evolution of the H10N3 influenza virus to ensure targeted prevention and control measures against influenza outbreaks.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 546-555"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000841/pdfft?md5=d1f4fe4ffce86ca2a5944e97cfc3a2eb&pid=1-s2.0-S1995820X24000841-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141318426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA viromes of Dermacentor nuttalli ticks reveal a novel uukuvirus in Qīnghăi Province, China 果蝇蜱的RNA病毒组揭示了中国青海省的一种新型uukuvirus。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.04.006

{"title":"RNA viromes of Dermacentor nuttalli ticks reveal a novel uukuvirus in Qīnghăi Province, China","authors":"","doi":"10.1016/j.virs.2024.04.006","DOIUrl":"10.1016/j.virs.2024.04.006","url":null,"abstract":"<div><p>Ticks are a major parasite on the Qīnghăi-Tibet Plateau, western China, and represent an economic burden to agriculture and animal husbandry. Despite research on tick-borne pathogens that threaten humans and animals, the viromes of dominant tick species in this area remain unknown. In this study, we collected <em>Dermacentor nuttalli</em> ticks near Qīnghăi Lake and identified 13 viruses belonging to at least six families through metagenomic sequencing. Four viruses were of high abundance in pools, including Xīnjiāng tick-associated virus 1 (XJTAV1), and three novel viruses: Qīnghăi Lake virus 1, Qīnghăi Lake virus 2 (QHLV1, and QHLV2, unclassified), and Qīnghăi Lake virus 3 (QHLV3, genus <em>Uukuvirus</em> of family <em>Phenuiviridae</em> in order <em>Bunyavirales</em>), which lacks the M segment. The minimum infection rates of the four viruses in the tick groups were 8.2%, 49.5%, 6.2%, and 24.7%, respectively, suggesting the prevalence of these viruses in <em>D. nuttalli</em> ticks. A putative M segment of QHLV3 was identified from the next-generation sequencing data and further characterized for its signal peptide cleavage site, N-glycosylation, and transmembrane region. Furthermore, we probed the L, M, and S segments of other viruses from sequencing data of other tick pools by using the putative M segment sequence of QHLV3. By revealing the viromes of D. nuttalli ticks, this study enhances our understanding of tick-borne viral communities in highland regions. The putative M segment identified in a novel uukuvirus suggests that previously identified uukuviruses without M segments should have had the same genome organization as typical bunyaviruses. These findings will facilitate virus discovery and our understanding of the phylogeny of tick-borne uukuviruses.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 537-545"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X2400066X/pdfft?md5=1375c48ceec7f2b52a4692f9aa0ed578&pid=1-s2.0-S1995820X2400066X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140859075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudorabies virus VHS protein abrogates interferon responses by blocking NF-κB and IRF3 nuclear translocation 伪狂犬病毒VHS蛋白通过阻断NF-κB和IRF3核转位来削弱干扰素反应。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.05.009

{"title":"Pseudorabies virus VHS protein abrogates interferon responses by blocking NF-κB and IRF3 nuclear translocation","authors":"","doi":"10.1016/j.virs.2024.05.009","DOIUrl":"10.1016/j.virs.2024.05.009","url":null,"abstract":"<div><p>Herpesviruses antagonize host antiviral responses through a myriad of molecular strategies culminating in the death of the host cells. Pseudorabies virus (PRV) is a significant veterinary pathogen in pigs, causing neurological sequalae that ultimately lead to the animal's demise. PRV is known to trigger apoptotic cell death during the late stages of infection. The virion host shutdown protein (VHS) encoded by UL41 plays a crucial role in the PRV infection process. In this study, we demonstrate that UL41 inhibits PRV-induced activation of inflammatory cytokine and negatively regulates the cGAS-STING-mediated antiviral activity by targeting IRF3, thereby inhibiting the translocation and phosphorylation of IRF3. Notably, mutating the conserved amino acid sites (E192, D194, and D195) in the RNase domain of UL41 or knocking down UL41 inhibits the immune evasion of PRV, suggesting that UL41 may play a crucial role in PRV's evasion of the host immune response during infection. These results enhance our understanding of how PRV structural proteins assist the virus in evading the host immune response.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 587-599"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000786/pdfft?md5=ab0084e6985767723baecc91995a9cbc&pid=1-s2.0-S1995820X24000786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between circulating T follicular helper cell levels after infection and a decreased risk of COVID-19 re-infection 感染后循环 T 滤泡辅助细胞水平与 COVID-19 再感染风险降低之间的相关性

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.06.002

引用次数: 0

Detection of HBV DNA integration in plasma cell-free DNA of different HBV diseases utilizing DNA capture strategy 利用DNA捕获策略检测不同HBV疾病血浆无细胞DNA中的HBV DNA整合。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.06.003

{"title":"Detection of HBV DNA integration in plasma cell-free DNA of different HBV diseases utilizing DNA capture strategy","authors":"","doi":"10.1016/j.virs.2024.06.003","DOIUrl":"10.1016/j.virs.2024.06.003","url":null,"abstract":"<div><p>The landscape of hepatitis B virus (HBV) integration in the plasma cell-free DNA (cfDNA) of HBV-infected patients with different stages of liver diseases [chronic hepatitis B (CHB), liver cirrhosis (LC), and hepatocellular carcinoma (HCC)] remains unclear. In this study, we developed an improved strategy for detecting HBV DNA integration in plasma cfDNA, based on DNA probe capture and next-generation sequencing. Using this optimized strategy, we successfully detected HBV integration events in chimeric artificial DNA samples and HBV-infected HepG2-NTCP cells at day one post infection, with high sensitivity and accuracy. The characteristics of HBV integration events in the HBV-infected HepG2-NTCP cells and plasma cfDNA from HBV-infected individuals (CHB, LC, and HCC) were further investigated. A total of 112 and 333 integration breakpoints were detected in the HepG2-NTCP cells and 22 out of 25 (88%) clinical HBV-infected samples, respectively. <em>In vivo</em> analysis showed that the normalized number of support unique sequences (<em>nnsus</em>) in HCC was significantly higher than in CHB or LC patients (<em>P</em> values < 0.05). All integration breakpoints are randomly distributed on human chromosomes and are enriched in the HBV genome around nt 1800. The majority of integration breakpoints (61.86%) are located in the gene-coding region. Both non-homologous end-joining (NHEJ) and microhomology-mediated end-joining (MMEJ) interactions occurred during HBV integration across the three different stages of liver diseases. Our study provides evidence that HBV DNA integration can be detected in the plasma cfDNA of HBV-infected patients, including those with CHB, LC, or HCC, using this optimized strategy.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 655-666"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24000828/pdfft?md5=c59234e1ed45ce52f4e2926238f136d0&pid=1-s2.0-S1995820X24000828-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AIMP2 restricts EV71 replication by recruiting SMURF2 to promote the degradation of 3D polymerase AIMP2 通过招募 SMURF2 来促进 3D 聚合酶的降解，从而限制 EV71 的复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.06.009

{"title":"AIMP2 restricts EV71 replication by recruiting SMURF2 to promote the degradation of 3D polymerase","authors":"","doi":"10.1016/j.virs.2024.06.009","DOIUrl":"10.1016/j.virs.2024.06.009","url":null,"abstract":"<div><p>Hand, foot and mouth disease (HFMD), mainly caused by enterovirus 71 (EV71), has frequently occurred in the Asia-Pacific region, posing a significant threat to the health of infants and young children. Therefore, research on the infection mechanism and pathogenicity of enteroviruses is increasingly becoming important. The 3D polymerase, as the most critical RNA-dependent RNA polymerase (RdRp) for EV71 replication, is widely targeted to inhibit EV71 infection. In this study, we identified a novel host protein, AIMP2, capable of binding to 3D polymerase and inhibiting EV71 infection. Subsequent investigations revealed that AIMP2 recruits the E3 ligase SMURF2, which mediates the polyubiquitination and degradation of 3D polymerase. Furthermore, the antiviral effect of AIMP2 extended to the CVA16 and CVB1 serotypes. Our research has uncovered the dynamic regulatory function of AIMP2 during EV71 infection, revealing a novel antiviral mechanism and providing new insights for the development of antienteroviral therapeutic strategies.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 632-644"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24001081/pdfft?md5=8e9849117e1716838af0defc408ef1c7&pid=1-s2.0-S1995820X24001081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measures of insulin resistance and beta cell function before and after treatment of HCV infection 治疗 HCV 感染前后的胰岛素抵抗和 beta 细胞功能测量。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.06.007

{"title":"Measures of insulin resistance and beta cell function before and after treatment of HCV infection","authors":"","doi":"10.1016/j.virs.2024.06.007","DOIUrl":"10.1016/j.virs.2024.06.007","url":null,"abstract":"<div><p>The association between chronic HCV infection and type 2 diabetes mellitus (T2DM) has been established; however, there is limited research on β-cell function particularly in the pre-diabetic population. Here, we evaluated indices of β-cell function and insulin sensitivity across the spectrum from normal glucose tolerance to T2DM in individuals with and without chronic hepatitis C (CHC), and the effects of antiviral treatments on these variables. A total of 153 non-cirrhotic, non-fibrotic CHC patients with a BMI <25 were enrolled in the study. Among them, 119 were successfully treated with either direct acting antiviral (DAA) drugs or pegylated interferon/ribavirin (IFN/RBV) anti-HCV therapy. Fasting state- and oral glucose tolerance test (OGTT)-derived indexes were used to evaluate β-cell function and insulin sensitivity. Among all subjects, 19 (13%) had T2DM and 21% exhibited pre-diabetes including 8% isolated impaired fasting glucose (IFG) and 13% combined IFG and impaired glucose tolerance (IGT). Early and total insulin secretion adjusted for the degree of insulin resistance were decreased in pre-diabetic CHC patients compared to HCV-uninfected individuals. Viral eradication through DAA or IFN/RBV therapy demonstrated positive impacts on insulin sensitivity and β-cell function in CHC patients who achieved sustained virologic response (SVR), regardless of fasting or OGTT state. These findings emphasize the role of HCV in the development of β-cell dysfunction, while also suggesting that viral eradication can improve insulin secretion, reverse insulin resistance, and ameliorate glycemic control. These results have important implications for managing pre-diabetic CHC patients and could prevent diabetes-related clinical manifestations and complications.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 667-674"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24001056/pdfft?md5=0b6aa3ce41faac910b24c120f2e9888b&pid=1-s2.0-S1995820X24001056-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141477521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A quadrivalent norovirus vaccine based on a chimpanzee adenovirus vector induces potent immunity in mice 基于黑猩猩腺病毒载体的四价诺如病毒疫苗可诱导小鼠产生强效免疫力。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2024-08-01 DOI: 10.1016/j.virs.2024.07.002

{"title":"A quadrivalent norovirus vaccine based on a chimpanzee adenovirus vector induces potent immunity in mice","authors":"","doi":"10.1016/j.virs.2024.07.002","DOIUrl":"10.1016/j.virs.2024.07.002","url":null,"abstract":"<div><p>Norovirus (NoV) infection is a major cause of gastroenteritis worldwide. The virus poses great challenges in developing vaccines with broad immune protection due to its genetic and antigenic diversity. To date, there are no approved NoV vaccines for clinical use. Here, we aimed to develop a broad-acting quadrivalent NoV vaccine based on a chimpanzee adenovirus vector, AdC68, carrying the major capsid protein (VP1) of noroviral GI and GII genotypes. Compared to intramuscular (i.m.), intranasal (i.n.), or other prime-boost immunization regimens (i.m. + i.m., i.m. + i.n., i.n. + i.m.), AdC68-GI.1-GII.3 (E1)-GII.4-GII.17 (E3), administered via i.n. + i.n. induced higher titers of serum IgG antibodies and higher IgA antibodies in bronchoalveolar lavage fluid (BALF) and saliva against the four homologous VP1s in mice. It also significantly stimulated the production of blocking antibodies against the four genotypes. In response to re-stimulation with virus-like particles (VLP)-GI.1, VLP-GII.3, VLP-GII.4, and VLP-GII.17, the quadrivalent vaccine administered according to the i.n. + i.n. regimen effectively triggered specific cell-mediated immune responses, primarily characterized by IFN-γ secretion. Furthermore, the preparation of this novel quadrivalent NoV vaccine requires only a single recombinant adenovirus to provide broad preventive immunity against the major GI/GII epidemic strains, making it a promising vaccine candidate for further development.</p></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"39 4","pages":"Pages 675-684"},"PeriodicalIF":5.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1995820X24001123/pdfft?md5=c85be2d64b45a21de8d2d0d74f5ea576&pid=1-s2.0-S1995820X24001123-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0