Virologica Sinica最新文献_第8页

Current antiviral therapies and promising drug candidates against respiratory syncytial virus infection 目前抗呼吸道合胞病毒感染的抗病毒疗法和有希望的候选药物。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.01.003

Ziheng Feng , Zhengde Xie , Lili Xu

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Evaluating the performance of the PREDAC method in flu vaccine recommendations over the past decade (2013–2023) 评估过去十年（2013-2023）PREDAC方法在流感疫苗推荐中的表现

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.01.004

Yousong Peng , Lei Yang , Weijuan Huang , Mi Liu , Xiao Ding , Xiangjun Du , Yuelong Shu , Taijiao Jiang , Dayan Wang

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Sphingomyelin phosphodiesterase 3 as a novel host factor inhibiting hepatitis B virus transcription 鞘磷脂二酯酶3作为抑制乙型肝炎病毒转录的新宿主因子。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.02.004

Chen Li , Meng Zhou , Kaitao Zhao , Wenbo Liu , Chengliang Zhu , Xiaoming Cheng , Yuchen Xia

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Identification and genetic analysis of new ephemeroviruses in wild boars in China 中国野猪新蜉蝣病毒的鉴定与遗传分析。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.02.002

Zhongzhong Tu , Tong Wang , Yu Xu , Heting Sun , Peng Peng , Siyuan Qin , Changchun Tu

{"title":"Identification and genetic analysis of new ephemeroviruses in wild boars in China","authors":"Zhongzhong Tu , Tong Wang , Yu Xu , Heting Sun , Peng Peng , Siyuan Qin , Changchun Tu","doi":"10.1016/j.virs.2025.02.002","DOIUrl":"10.1016/j.virs.2025.02.002","url":null,"abstract":"<div><div>Ephemeroviruses (EVs) are arthropod-borne rhabdoviruses and were isolated exclusively from cattle and haematophagous arthropods until two new ephemeroviruses were first identified from domestic pigs most recently. Here we report the identification of newer EVs in wild boar by meta-transcriptomic (MTT) sequencing. Further screening by specific RT-nPCR of tissue samples of 459 free-ranging wild boars collected between 2018 and 2023 from 26 provinces across China confirmed five positive wild boars in four provinces. Interestingly, two ticks especially collected from two positive wild boars were also EV positive. Finally, four complete genome sequences of wild boar ephemeroviruses (WbEVs) were obtained with two strains belonging to a new EV species, and the rest two falling into porcine ephemerovirus 2 (PoEV2) species identified from domestic pigs. Our study has further extended EV host range and demonstrated natural circulations of divergent EVs in wild boars, in which ticks may play roles. Biological implications of EV infection in wild boars should be interesting topics for future investigations.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 186-191"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Artemisia annua L. leaf extracts suppress influenza virus infection by targeting the viral nucleoprotein and blocking mitochondria-mediated apoptosis 黄花蒿叶提取物通过靶向病毒核蛋白和阻断线粒体介导的细胞凋亡抑制流感病毒感染。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.001

Xiwen Zhao , Xuan Dai , Fuyi Wang , Chenyang Li , Xun Song , Yingying Han , Chaowei Zhang , Lu Wang , Zhendan He , Rongping Zhang , Liang Ye

{"title":"Artemisia annua L. leaf extracts suppress influenza virus infection by targeting the viral nucleoprotein and blocking mitochondria-mediated apoptosis","authors":"Xiwen Zhao , Xuan Dai , Fuyi Wang , Chenyang Li , Xun Song , Yingying Han , Chaowei Zhang , Lu Wang , Zhendan He , Rongping Zhang , Liang Ye","doi":"10.1016/j.virs.2025.03.001","DOIUrl":"10.1016/j.virs.2025.03.001","url":null,"abstract":"<div><div><em>Artemisia annua</em> L. is a medicinal herb with multiple therapeutic applications, whereas its anti-influenza A virus (IAV) efficiency and mechanism of action are still unclear. Here, we investigated the inhibition activity and mechanism of <em>A. annua</em> leaf methanol extracts (AALME) against IAV <em>in vitro</em> and <em>in vivo</em>. Our results revealed that AALME exhibits potent anti-IAV activity by interacting with IAV particles. Mechanistically, AALME directly targets the IAV nucleoprotein (NP) protein and abolishes the nuclear import of IAV NP. AALME profoundly suppresses IAV-induced mitochondrial apoptosis via suppressing ROS-mediated AIF-dependent pathways. More importantly, we found that AALME plays a crucial role in protecting mice from IAV infection and mitigating IAV pathogenicity. This current work provides mechanistic insight into the mechanism by which AALME controls IAV infection <em>in vitro</em> and <em>in vivo</em>, potentially contributing to the development of antiviral treatments for IAV infection.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 247-259"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Issue Cover 覆盖问题

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/S1995-820X(25)00047-1

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Characterization of novel highly pathogenic avian influenza A(H5N6) clade 2.3.4.4b virus in wild birds, East China, 2024 中国东部地区野生鸟类新型高致病性禽流感A（H5N6）分支2.3.4.4b病毒特征分析

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.01.002

Renjie Sun , Xiaoxiao Feng , Jing Huang , Fangyu Zheng , Ronghui Xie , Chuanliang Zhang , Hongli Zhang , Yin Xue , Aijun Liu , Xiaobing Huang , Lin Yuan , Lingyan Zhao

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Identification of an immunodominant neutralizing epitope of porcine astrovirus type 5 capsid protein 猪星状病毒5型衣壳蛋白免疫显性中和表位的鉴定。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.003

Yixin Hu , Zehui Li , Chenlin Hao , Hao Lu , Yunfei Xing , Kexin Liu , Xiaohui Jin , Zhanyong Wei

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Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies 用于抗病毒药物筛选和生命周期研究的报告毒株HBoV1的研制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.009

Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen

{"title":"Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies","authors":"Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen","doi":"10.1016/j.virs.2025.03.009","DOIUrl":"10.1016/j.virs.2025.03.009","url":null,"abstract":"<div><div>Human bocavirus 1 (HBoV1; family: <em>Parvoviridae</em>) causes a wide spectrum of respiratory diseases in children and gastroenteritis in adults. A lack of sensitive cell lines and efficient animal models hinders research on HBoV, including the development of anti-HBoV drugs or vaccines. Although the construction of a wild-type HBoV1 infectious clone has been reported, generating HBoV1 infectious clone carrying foreign reporter genes with suitable insertion sites in its genome while retaining replicative ability remains challenging. Here, HBoV1 infectious clones harboring the 11-amino-acid HiBiT tag at five distinct insertion sites were constructed and evaluated. Only the recombinant HBoV1 carrying the HiBiT tag in the N-terminus of the NS1 protein (HBoV1-HiBiT<sub>NS1</sub>) displayed comparable characteristics to wild-type HBoV1 as determined via the analysis of viral DNA copy number, NanoLuc activity, viral protein expression, and the formation of replication intermediates. Notably, the replication kinetics of HBoV1-HiBiT<sub>NS1</sub> could be examined by monitoring NanoLuc activity, which was noted to be correlated with the viral DNA level. Additionally, we successfully applied HiBiT-tagged HBoV1 for the evaluation of antiviral drug activity and identified ivermectin (EC50 = 2.27 μM) as a potent anti-HBoV1 replication drug. Overall, our study demonstrated that the HBoV1-HiBiT<sub>NS1</sub> reporter can serve as a convenient platform for screening candidate drugs targeting HBoV1 replication and may also be useful for investigating the life cycle of the virus.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 275-283"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4+ T cell 宿主因子 Naf1 限制了 HIV-1 对髓细胞的感染，并削弱了树突状细胞为 CD4+ T 细胞提供能量的能力。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.007

Chengzuo Xie , Xia Jin , Wan-Wei Li , Jian-Hua Wang

{"title":"Host factor Naf1 restricts HIV-1 infection of myeloid cells and compromises the capacity of dendritic cell to prime CD4+ T cell","authors":"Chengzuo Xie , Xia Jin , Wan-Wei Li , Jian-Hua Wang","doi":"10.1016/j.virs.2025.03.007","DOIUrl":"10.1016/j.virs.2025.03.007","url":null,"abstract":"<div><div>Naf1 (Nef-associated factor 1) is a host protein that interacts with human immunodeficiency virus type 1 (HIV-1) Nef protein. We and others have previously demonstrated that Naf1 restricts HIV-1 infection of T-lymphocytes. Myeloid cells are targets for HIV infection, but Naf1 expression in myeloid cells and whether it also regulates HIV infection in these cells are not yet identified. In this study, we found that Naf1 had a higher expression in CD14<sup>+</sup> monocytes than in monocyte-derived dendritic cells (MDDCs), and its expression in both types of cells could be induced by HIV-1 gp120 glycoproteins or viral particles. Importantly, the expression of Naf1 restricted HIV-1 infection in monocytes and MDDCs. Functional investigation showed that both the constitutive and the induced expression of Naf1 inhibited NF-κB signaling in MDDCs and reduced the basal level or LPS (Lipopolysaccharide)-stimulated production of cytokines. Moreover, Naf1 reduced the expression of ICAM-1 (intercellular cell adhesion molecule-1) on MDDCs and compromised their capacity to prime the activation of resting CD4<sup>+</sup> T cells in co-culture. In light of the essential role of NF-κB signaling for HIV-1 transcription, Naf1-mediated inhibition of NF-κB signaling may hinder a robust viral replication in MDDCs and help maintain viral persistence. Furthermore, virus-induced Naf1 expression in MDDCs may diminish the cross-talk between DC (dendritic cell) and T cells, hence suppressing the activation of antiviral immune responses. Taken together, we identified the new function of Naf1 in myeloid cells. Those findings may facilitate the understanding for the host restriction of HIV-1 infection in myeloid cells.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 217-224"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0