Virologica Sinica最新文献

Nucleophosmin 1 inhibits the replication of influenza A virus by competitively binding viral RNA with viral proteins. 核磷蛋白1通过竞争性结合病毒RNA与病毒蛋白抑制甲型流感病毒的复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-05-08 DOI: 10.1016/j.virs.2025.04.007

Yingying Yu, Qian Wang, Yanli Wei, Junwen Liu, Guangwen Wang, Zhengxiang Wang, Wentao Shen, Lu Han, Chengjun Li, Cao-Qi Lei, Shuai Xu, Qiyun Zhu

引用次数: 0

A Mouse Model of Crimean-Congo Hemorrhagic Fever Virus-induced Coagulopathy. 克里米亚-刚果出血热病毒致凝血病小鼠模型。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-30 DOI: 10.1016/j.virs.2025.04.008

Hui Zhang, Ziyang Jiang, Haidang Liao, Jiang Li, Manli Wang, Yiwu Zhou, Zhihong Hu, Jia Liu

引用次数: 0

A noninfectious pseudovirus system for an emerging orthoflavivirus. 新出现的正黄病毒的非传染性假病毒系统。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-23 DOI: 10.1016/j.virs.2025.04.006

Yanfan Shi, Yu He, Xiaoli Wang, Zhen Wu, Tao Wang, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Di Sun, Anchun Cheng, Shun Chen

引用次数: 0

Viral pseudo-enzyme facilitates KSHV lytic replication via suppressing PFAS-mediated RTA deamidation. 病毒伪酶通过抑制pfas介导的RTA脱酰胺促进KSHV裂解复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-12 DOI: 10.1016/j.virs.2025.04.005

Yang Xu, Qiushi Zhang, Guoli Hou, Liang Hu, Tiaoyi Xiao, Xinyu Liang, Deliang Li, Junhua Li

{"title":"Viral pseudo-enzyme facilitates KSHV lytic replication via suppressing PFAS-mediated RTA deamidation.","authors":"Yang Xu, Qiushi Zhang, Guoli Hou, Liang Hu, Tiaoyi Xiao, Xinyu Liang, Deliang Li, Junhua Li","doi":"10.1016/j.virs.2025.04.005","DOIUrl":"https://doi.org/10.1016/j.virs.2025.04.005","url":null,"abstract":"<p><p>Deamidation, a type of post-translational modification commonly considered a hallmark of protein \"aging\" and function decay, is increasingly recognized for its pivotal role in regulating biological processes and viral infection. Our previous study has demonstrated that the deamidation of replication and transcription activator (RTA), a master regulator of ubiquitous and oncogenic Kaposi's sarcoma-associated herpesvirus (KSHV), mediated by phosphoribosylformylglycinamidine synthetase (PFAS), hinders its nuclear import and transcriptional activity. Here we report that the viral glutamine amidotransferase (vGAT) pseudo-enzyme was exploited to facilitate KSHV lytic infection by inhibiting RTA deamidation. To be more specific, vGAT interacted with both RTA and cellular PFAS, and inhibited PFAS-mediated RTA deamidation, thus facilitating RTA nuclear localization and suppressing nuclear factor-kappa B (NF-κB) signaling activation, as well as augmenting RTA-mediated transcriptional activation of viral open reading frames (ORFs). In addition, vGAT appeared to regulate the deamidation process of several viral ORFs of KSHV. Collectively, these findings unveil that a viral pseudo-enzyme was exploited to enhance viral infection via deamidation regulation.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scutellaria barbata D. Don extracts alleviate SARS-CoV-2 induced acute lung injury by inhibiting virus replication and bi-directional immune modulation. 黄芩提取物通过抑制病毒复制和双向免疫调节减轻SARS-CoV-2诱导的急性肺损伤。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-12 DOI: 10.1016/j.virs.2025.04.004

Yan Ran, Zinuo Chen, Carolina Q Sacramento, Lingyuan Fan, Qinghua Cui, Lijun Rong, Ruikun Du

{"title":"Scutellaria barbata D. Don extracts alleviate SARS-CoV-2 induced acute lung injury by inhibiting virus replication and bi-directional immune modulation.","authors":"Yan Ran, Zinuo Chen, Carolina Q Sacramento, Lingyuan Fan, Qinghua Cui, Lijun Rong, Ruikun Du","doi":"10.1016/j.virs.2025.04.004","DOIUrl":"https://doi.org/10.1016/j.virs.2025.04.004","url":null,"abstract":"<p><p>The emergence of SARS-CoV-2 variants and drug-resistant mutants emphasizes the urgent need to develop novel antiviral agents. In the present study, we examined the therapeutic effect of the Chinese medicinal herb, Scutellaria barbata D. Don (SBD), against SARS-CoV-2 infection both in vitro and in vivo. Using a viral replicon particle (VRP)-based mouse model of SARS-CoV-2 infection, our study revealed that SBD extracts can reduce viral load in mouse lungs and alleviate the viral induced pneumonia. In vitro antiviral determination further validated the direct acting antiviral efficacy of SBD extracts against SARS-CoV-2 replication. Mechanistic studies demonstrated that SBD can act against SARS-CoV-2 replication by targeting both 3-chymotrypsin-like and papain-like cysteine proteases, via a combination of multiple active constituents. Moreover, SBD can modulate the host inflammation response in a bi-directional manner, which also contribute to the mitigation of viral induced acute lung injury. In summary, our study provides SBD as a promising therapeutic agent to combat SARS-CoV-2 infections that merit further development.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coadministration of ribavirin and arenaviral entry inhibitor LHF-535 enhances antiviral benefit against authentic lassa virus. 利巴韦林和沙粒病毒进入抑制剂LHF-535联合使用可增强对真正拉沙病毒的抗病毒作用。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-12 DOI: 10.1016/j.virs.2025.04.003

Cheng Peng, Jialing Hu, Yuan Bai, Wei Wu, Wenting Mao, Yang Liu, Yi Wan, Lei Zhang, Wei Li, Tingting Tian, Tiezhu Liu, Yanhai Wang, Mifang Liang, Jun Han, Zhiming Yuan, Jiandong Li, Chao Shan, Fei Deng, Wei Wang

引用次数: 0

Differential susceptibility of immunodeficient mice to MPXV infection and the impact of various inoculation routes. 免疫缺陷小鼠对MPXV感染的差异易感性及不同接种途径的影响。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-07 DOI: 10.1016/j.virs.2025.04.001

Xiaohan Wang, Shaowen Shi, Xiaoxuan Nie, Yongyang Sun, Jinglei Hu, Manlin He, Wenhao Ren, Yuxing Wang, Zhendong Guo, Gonghe Li, Changbo Ou, Xiao Li, Zongzheng Zhao

{"title":"Differential susceptibility of immunodeficient mice to MPXV infection and the impact of various inoculation routes.","authors":"Xiaohan Wang, Shaowen Shi, Xiaoxuan Nie, Yongyang Sun, Jinglei Hu, Manlin He, Wenhao Ren, Yuxing Wang, Zhendong Guo, Gonghe Li, Changbo Ou, Xiao Li, Zongzheng Zhao","doi":"10.1016/j.virs.2025.04.001","DOIUrl":"https://doi.org/10.1016/j.virs.2025.04.001","url":null,"abstract":"<p><p>Monkeypox virus (MPXV), a member of the Orthopoxvirus genus, caused a large-scale global outbreak in 2022. Developing mouse models for MPXV infection is crucial for advancing research on vaccines and therapeutic interventions. To address this, we conducted a comparative study on the susceptibility of six mouse strains-severe combined immune-deficiency (SCID), nude, genetically diabetic (db/db) and obese (ob/ob), C57BL/6J, and BALB/c-to MPXV infection. Mouse strains were infected with MPXV via intranasal inoculation, and body weight changes and mortality were monitored post-infection. Additionally, the tissue distribution of MPXV and the pathological changes in the lung tissues of the infected mice were evaluated. The results demonstrated that SCID and nude mice exhibited significant weight loss following MPXV infection, with 100 % mortality observed in SCID mice, while no mortality occurred in nude mice. In contrast, the other mouse strains showed no significant weight loss or mortality. Notably, the viral load in the lung tissues of SCID and nude mice was the highest among the tested strains. Furthermore, we investigated the impact of different inoculation routes-intranasal (I.N.), intraperitoneal (I.P.), and intravenous (I.V.)-on the pathogenicity of MPXV in mice. The results revealed that the intravenous route induced more pronounced pathogenic effects compared to the intranasal and intraperitoneal routes. In summary, this study provides valuable insights into the development of MPXV-infected mouse models, offering a foundation for further research on MPXV pathogenesis and therapeutic drug development.</p>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WITHDRAWN: Comparative metatranscriptome analysis in gut reveals insignificant host or microbiota changes in SARS-related coronavirus naturally infected bats. 撤回：肠道的比较转录组分析显示，在sars相关冠状病毒自然感染的蝙蝠中，宿主或微生物群的变化微不足道。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-07 DOI: 10.1016/j.virs.2025.04.002

Qi Wang, Shi-Qiang Mei, Tian-Yi Dong, Jia Su, Yuan-Fei Pan, Yan Zhu, Ke Wu, Li-Biao Zhang, Mang Shi, Peng Zhou

引用次数: 0

The SARS-CoV-2 NSP4 T492I mutation promotes double-membrane vesicle formation to facilitate transmission SARS-CoV-2 NSP4 T492I 突变促进了双膜囊泡的形成，从而有利于传播。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.010

Pei Wang , Buyun Tian , Ke Xiao , Wei Ji , Zonghong Li

{"title":"The SARS-CoV-2 NSP4 T492I mutation promotes double-membrane vesicle formation to facilitate transmission","authors":"Pei Wang , Buyun Tian , Ke Xiao , Wei Ji , Zonghong Li","doi":"10.1016/j.virs.2025.03.010","DOIUrl":"10.1016/j.virs.2025.03.010","url":null,"abstract":"<div><div>The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in mutations not only in the spike protein, aiding immune evasion, but also in the NSP3/4/6 proteins, crucial for regulating double-membrane vesicle (DMV) formation. However, the functional consequences of these NSP3/4/6 mutations remain poorly understood. In this study, a systematic analysis was conducted to investigate the evolutionary patterns of NSP3/4/6 mutations and their impact on DMV formation. The findings revealed that the NSP4 T492I mutation, a prevalent mutation found in all Delta and Omicron sub-lineages, notably enhances DMV formation. Mechanistically, the NSP4 T492I mutation enhances its homodimerization, leading to an increase in the size of puncta induced by NSP3/4, and also augments endoplasmic reticulum (ER) membrane curvature, resulting in a higher DMV density per fluorescent puncta. This study underscores the significance of the NSP4 T492I mutation in modulating DMV formation, with potential implications for the transmission dynamics of SARS-CoV-2. It contributes valuable insights into how these mutations impact viral replication and pathogenesis.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 225-235"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-function insights into the dual role of African swine fever virus pB318L: A typical geranylgeranyl-diphosphate synthase and a nuclear import protein 非洲猪瘟病毒pB318L的结构-功能双重作用：一种典型的香叶二磷酸合成酶和一种核输入蛋白。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.013

Hai-Fan Zhao , Ying Wang , Xiao-Hong Liu , Xian-Hui Liu , Zhi Geng , Zeng-Qiang Gao , Li Huang , Chang-Jiang Weng , Yu-Hui Dong , Heng Zhang

{"title":"Structure-function insights into the dual role of African swine fever virus pB318L: A typical geranylgeranyl-diphosphate synthase and a nuclear import protein","authors":"Hai-Fan Zhao , Ying Wang , Xiao-Hong Liu , Xian-Hui Liu , Zhi Geng , Zeng-Qiang Gao , Li Huang , Chang-Jiang Weng , Yu-Hui Dong , Heng Zhang","doi":"10.1016/j.virs.2025.03.013","DOIUrl":"10.1016/j.virs.2025.03.013","url":null,"abstract":"<div><div>African swine fever virus (ASFV) pB318L is an important protein for viral replication that acts as a membrane-bound <em>trans</em>-geranylgeranyl-diphosphate synthase (GGPPS) catalyzing the condensation of isopentenyl diphosphate (IPP) with allylic diphosphates. Recently we solved the crystal structure pB318L lacking N-terminal transmembrane region and performed a preliminary structural analysis. In this study, structure-based mutagenesis study and geranylgeranyl pyrophosphate (GGPP) production assay further revealed the key residues for the GGPPS activity. Structural comparison showed pB318L displays a strong similarity to typical GGPPSs instead of protein prenyltransferases. The phylogenetic analysis indicated pB318L may share a common ancestor with the GGPPSs from <em>Brassicaceae</em> plants rather than from its natural host. The subcellular localization analysis showed pB318L is localized in both nucleus and cytoplasm (including the endoplasmic reticulum membrane and mitochondria outer membrane). A unique N-terminal nuclear localization signal (NLS) following the transmembrane region was discovered in pB318L and the NLS was confirmed to be required for the nuclear import. We further revealed the NLS plays an essential role in the interaction with nuclear transporter karyopherin subunit alpha 1 (KPNA1). Their interaction may suppress signal transducers and activators of transcription 1 (STAT1) translocation and subsequently competitively inhibit nuclear import of IFN-stimulated gene factor 3 (ISGF3) complex. Our biochemical, structural and cellular analyses provide novel insights to pB318L that acts as an essential GGPPS that promotes viral replication and as a nuclear import protein that may be involved in immune evasion of ASFV.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 236-246"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0