Virologica Sinica最新文献

Thermal tolerance and inactivation of Ebola virus. 埃博拉病毒的耐热性和灭活性。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-09 DOI: 10.1016/j.virs.2025.07.004

Xiaoxiao Gao, Cheng Peng, Rongjuan Pei

引用次数: 0

SNX10 enhances HCoV-OC43 infection by facilitating viral entry and inhibiting virus-triggered autophagy. SNX10通过促进病毒进入和抑制病毒引发的自噬来增强HCoV-OC43感染。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-09 DOI: 10.1016/j.virs.2025.07.005

Haobin Li, Huiyi Guo, Binhao Rong, Haowei Li, Wenjiao Wu, Chan Yang, Shuwen Liu

{"title":"SNX10 enhances HCoV-OC43 infection by facilitating viral entry and inhibiting virus-triggered autophagy.","authors":"Haobin Li, Huiyi Guo, Binhao Rong, Haowei Li, Wenjiao Wu, Chan Yang, Shuwen Liu","doi":"10.1016/j.virs.2025.07.005","DOIUrl":"https://doi.org/10.1016/j.virs.2025.07.005","url":null,"abstract":"The ongoing coronavirus epidemic, including the novel coronavirus (COVID-19), continues to pose a significant threat to global public health. Host targets address multiple stages of the viral life cycle and provide diverse opportunities for therapeutic interventions. This study identified sorting nexin 10 (SNX10) as a facilitator of replication of human coronavirus OC43 (HCoV-OC43), underscoring its potential as a novel antiviral target. The knockout of SNX10 significantly suppressed HCoV-OC43 replication both in vivo and in vitro. Immunoprecipitation-mass spectrometry (‌IP-MS) analysis identified the adaptor protein complex 2 subunit μ1 (AP2M1) as a direct interactor of SNX10. Specifically, SNX10 facilitates phosphorylation of the AP2M1, thereby enhancing clathrin-mediated viral endocytosis. Furthermore, subsequent binding and internalization assays revealed that SNX10 knockout significantly inhibits viral entry into host cells. Conversely, the reconstitution of SNX10 fully restored viral entry, thereby confirming the critical and indispensable role of SNX10 in pathogen internalization. Simultaneously, SNX10 was identified as a key factor that promotes endosomal acidification by modulating pH levels, which in turn facilitated the release of the viral genome. Notably, the ablation of SNX10 was found to trigger autophagy activation during infection, thereby maintaining intracellular homeostasis. Additionally, it exerted autonomous antiviral effects through lysosomal degradation pathways. Collectively, these findings demonstrate SNX10 serves as a pivotal regulator of the viral life cycle and underscore its therapeutic potential as a multi-faceted antiviral candidate capable of simultaneously inhibiting viral internalization, viral genomic release, and host-pathogen equilibrium.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144620767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Coronavirus 3CL protease: Unveiling Its Complex Host Interactions and Central Role in Viral Pathogenesis. 冠状病毒3CL蛋白酶：揭示其复杂的宿主相互作用及其在病毒发病中的核心作用。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-07 DOI: 10.1016/j.virs.2025.07.002

Yecheng Zhang, Xinlei Ji, Dan Huang, Gen Lu, Xinwen Chen

引用次数: 0

STAT5-c-Myc-axis regulates B cell metabolism in vaccinated individuals and COVID-19 recovered patients. stat5 -c- myc轴调节接种疫苗个体和COVID-19康复患者的B细胞代谢。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-07 DOI: 10.1016/j.virs.2025.07.003

Lu Yang, Linhua Wang, Qian Liu, Xu Zhang, Yuexin Luo, Junbiao Xue, Xinpu Yang, Maria G Byazrova, Alexander V Filatov, Sheng-Ce Tao, Wei Xiao, Chaohong Liu

{"title":"STAT5-c-Myc-axis regulates B cell metabolism in vaccinated individuals and COVID-19 recovered patients.","authors":"Lu Yang, Linhua Wang, Qian Liu, Xu Zhang, Yuexin Luo, Junbiao Xue, Xinpu Yang, Maria G Byazrova, Alexander V Filatov, Sheng-Ce Tao, Wei Xiao, Chaohong Liu","doi":"10.1016/j.virs.2025.07.003","DOIUrl":"https://doi.org/10.1016/j.virs.2025.07.003","url":null,"abstract":"SARS-CoV-2 infection and vaccination both trigger immune responses. The former leads to naturally acquired immunity, while the latter induces active immunity through artificial means. However, the distinct immune effects of vaccination and infection, as well as their underlying mechanisms, require further clarification. In this study, we compared the peripheral B cell differentiation, serological differences and the expression level of BCR signaling molecules between the vaccinated and recovered group. The vaccinated group exhibited reduced RBD-specific B cell differentiation and lower CD86 signal intensity on memory B cells, but enhanced BCR signaling in B cells. Regarding metabolic signaling, the vaccinated group had elevated expression levels of pS6, c-Myc, pmTOR, and pSTAT5, suggesting that the STAT5-c-Myc axis plays a role in regulating B cell metabolism. Additionally, proteome microarray analysis revealed that the serum of the vaccinated group contained higher levels of IgG antibodies against the SARS-CoV-2 N-Nter protein and IgA antibodies specific to the SARS-CoV-2 S1 protein. In summary, these findings indicate that the vaccinated group develops a more robust coronavirus-specific immune response, with enhanced BCR signaling and metabolic activity compared to the recovered group. These insights might contribute to the optimization of SARS-CoV-2 vaccine design.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a novel ectromelia virus from rodent: Implications for use as an in vivo infection model for vaccine and antiviral research. 从啮齿动物中鉴定出一种新的鼠爪病毒：用于疫苗和抗病毒研究的体内感染模型的意义。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-07 DOI: 10.1016/j.virs.2025.07.001

Shuting Huo, Changcheng Wu, Zhenyong Qi, Jiewei Sun, Xin Meng, Jingdong Song, Zhongxian Zhang, Liye Jin, Chang Shu, Zhifeng Lin, Weibang Huo, Yao Deng, Li Zhao, Jiandong Li, Wenjie Tan

{"title":"Identification of a novel ectromelia virus from rodent: Implications for use as an in vivo infection model for vaccine and antiviral research.","authors":"Shuting Huo, Changcheng Wu, Zhenyong Qi, Jiewei Sun, Xin Meng, Jingdong Song, Zhongxian Zhang, Liye Jin, Chang Shu, Zhifeng Lin, Weibang Huo, Yao Deng, Li Zhao, Jiandong Li, Wenjie Tan","doi":"10.1016/j.virs.2025.07.001","DOIUrl":"https://doi.org/10.1016/j.virs.2025.07.001","url":null,"abstract":"Ectromelia virus (ECTV), a member of the Orthopoxvirus genus, serves as both a causative agent of mousepox and a pivotal surrogate model for studying highly pathogenic orthopoxviruses. While genomic data on ECTV remains limited, we report the isolation and characterization of a novel strain, ECTV-C-Tan-GD01, from rodents in Guangdong Province, China. Nanopore sequencing yielded a complete genome (199 annotated genes, including one gene truncated at the C-terminus) with inverted terminal repeats (ITRs) harboring a conserved hairpin structure. Notably, a frameshift-inducing \"G\" deletion in the EV159 gene resulted in the truncation of a semaphorin-like protein. In vitro assays demonstrated cell-associated viral replication kinetics, with maximum titers achieved earlier in Vero/HeLa cells (72 h) than in BHK-21/CEF cells (84 h). Murine challenge experiments revealed extreme virulence (LD50 < 1 plaque-forming unit (PFU) via intranasal/footpad routes) and hepatosplenic tropism. Furthermore, ECTV-C-Tan-GD01 exhibited utility in evaluating orthopoxvirus countermeasures: a single dose of vaccinia virus Tiantan (VTT) or non-replicating vaccinia virus Tiantan (NTV) conferred cross-protection, while tecovirimat (ST-246), cidofovir (CDV), and brincidofovir (initially CMX001) significantly reduced viral loads and pathology. This study establishes ECTV-C-Tan-GD01 as a dual-purpose resource for probing orthopoxvirus evolution and advancing therapeutic development.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cycle Rift Valley fever virus particles from stable replicon cells enable discovery of antiviral CNX-1351 for multiple RNA viruses. 来自稳定复制子细胞的单循环裂谷热病毒颗粒使多种RNA病毒的抗病毒药物CNX-1351得以发现。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-04 DOI: 10.1016/j.virs.2025.06.009

Zhichao Gao, Hongyuan Guo, Ziqiao Wang, Pengcheng Wang, Xinran Sun, Shimei Zhang, Fei Feng, Chao Shan, Youhua Xie, Rong Zhang

{"title":"Single-cycle Rift Valley fever virus particles from stable replicon cells enable discovery of antiviral CNX-1351 for multiple RNA viruses.","authors":"Zhichao Gao, Hongyuan Guo, Ziqiao Wang, Pengcheng Wang, Xinran Sun, Shimei Zhang, Fei Feng, Chao Shan, Youhua Xie, Rong Zhang","doi":"10.1016/j.virs.2025.06.009","DOIUrl":"https://doi.org/10.1016/j.virs.2025.06.009","url":null,"abstract":"Rift Valley fever virus (RVFV) is a high-containment pathogen that causes severe diseases in humans, with no approved therapeutics available. Its classification as a biosafety level 3 (BSL-3) agent has limited research and therapeutic development due to safety concerns. In this study, we developed a stable replicon cell line maintaining the replication of L and S genomic segments of RVFV. Single-cycle viral replicon particles (VRPs) could be efficiently packaged through trans-complementation of glycoproteins from different strains, recapitulating authentic viral entry and replication while minimizing biosafety risks. Using this system, we conducted high-throughput screening of a small-molecule compound library and identified CNX-1351 as an antiviral agent for multiple RNA viruses. Mechanistic studies revealed that CNX-1351 inhibits viral replication, potentially by targeting the PI3K-Akt signaling pathway. This single-cycle VRP system provides a valuable tool for studying RVFV biology, host interactions, antiviral and vaccine development under reduced biosafety constraints.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical outcomes after HBsAg clearance in chronic hepatitis B patients treated with Peg-IFN α:A study with an 11- to 173-month follow-up. Peg-IFN α治疗慢性乙型肝炎患者HBsAg清除后的临床结果：一项随访11至173个月的研究

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-04 DOI: 10.1016/j.virs.2025.06.008

Wen Deng, Hongxiao Hao, Ziyu Zhang, Xinxin Li, Weihua Cao, Yaqin Zhang, Shiyu Wang, Zixuan Gao, Linmei Yao, Shuojie Wang, Xin Wei, Wei Yi, Linqing Zhao, Yao Xie, Minghui Li

{"title":"Clinical outcomes after HBsAg clearance in chronic hepatitis B patients treated with Peg-IFN α:A study with an 11- to 173-month follow-up.","authors":"Wen Deng, Hongxiao Hao, Ziyu Zhang, Xinxin Li, Weihua Cao, Yaqin Zhang, Shiyu Wang, Zixuan Gao, Linmei Yao, Shuojie Wang, Xin Wei, Wei Yi, Linqing Zhao, Yao Xie, Minghui Li","doi":"10.1016/j.virs.2025.06.008","DOIUrl":"https://doi.org/10.1016/j.virs.2025.06.008","url":null,"abstract":"To investigate the risk and influencing factors of long-term liver adverse events in chronic hepatitis B patients achieving hepatitis B surface antigen (HBsAg) clearance after pegylated interferon α (Peg-IFN α) treatment, a retrospective analysis was conducted on 456 patients at Beijing Ditan Hospital from 2008 to 2023 who achieved HBsAg clearance and discontinued Peg-IFN α treatment. The baseline was defined as the time of HBsAg clearance and treatment cessation. The endpoint was the first occurrence of liver adverse events (hepatocellular carcinoma or ascites) or last follow-up. Subsequently, we evaluated the incidence and risk factors of liver adverse events, along with changes in liver fibrosis, cirrhosis, and liver function indicators. During a median follow-up of 70 months, the incidence of liver adverse events was 2.30%, hepatocellular carcinoma 1.76%, and ascites 0.55%. Older age and cirrhosis were significant risk factors (HR 1.075 and 41.393, both P < 0.01). The APRI score significantly improved at follow-up compared to baseline (0.53 vs. 0.25, P < 0.001), and cirrhosis prevalence decreased from 5.70% to 0.88% (P < 0.001). In conclusion, patients who achieved HBsAg clearance and discontinued Peg-IFN α treatment have a low risk of liver adverse events, while advanced age and cirrhosis remain major risk factors.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2-encoded miR-nsp3-3p promotes pulmonary fibrosis by inhibiting expression of ALCAM. sars - cov -2编码的miR-nsp3-3p通过抑制ALCAM的表达促进肺纤维化。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-01 DOI: 10.1016/j.virs.2025.06.007

Yang Yang, Qiuyue Wu, Xueyan Liu, Hongjian Zhou, Jianzhen Lei, Lan Luo, Xinyi Xia

{"title":"SARS-CoV-2-encoded miR-nsp3-3p promotes pulmonary fibrosis by inhibiting expression of ALCAM.","authors":"Yang Yang, Qiuyue Wu, Xueyan Liu, Hongjian Zhou, Jianzhen Lei, Lan Luo, Xinyi Xia","doi":"10.1016/j.virs.2025.06.007","DOIUrl":"https://doi.org/10.1016/j.virs.2025.06.007","url":null,"abstract":"microRNAs (miRNAs) derived from viruses, have been detected in body fluids and are known to regulate the expression of host genes. Recent evidence indicates that SARS-CoV-2-encoded miRNAs could contribute to pulmonary disease. Pulmonary fibrosis is an important complication in SARS-CoV-2 infected patients, either during hospitalization or after discharge, however, the underlying mechanisms are not fully elucidated. Here, we report a SARS-CoV-2-encoded miRNA, miR-nsp3-3p, facilitates host pulmonary fibrosis by inhibiting expression of activated leukocyte cell adhesion molecule (ALCAM) and promoting epithelial-mesenchymal transition (EMT). First, we detected miR-nsp3-3p in clinical specimens and found it was remarkably increased in throat swabs and alveolar lavage fluids from severe/critical COVID-19 patients compared to controls or mild/moderate patients. We further revealed that adeno-associated virus(AAV)-nsp3 infection can induce pulmonary fibrosis in BALB/c mice while miR-nsp3-3p antagomirs can reverse that, and ALCAM was found to be as a target gene of miR-nsp3-3p. miR-nsp3-3p overexpression can inhibit the expression of ALCAM and promote EMT of pulmonary epithelial cells. Moreover, overexpression of ALCAM can reverse the miR-nsp3-3p-induced EMT and fibrosis. These findings highlight the essential role of SARS-CoV-2-encoded miRNAs in promoting the pathological progression of lung disease, and provide novel insights into the interactions between viral miRNAs and host pathology.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and accurate detection of infectious SARS-CoV-2 by viral receptor capture combined with loop-mediated isothermal amplification. 病毒受体捕获结合环介导等温扩增快速准确检测传染性SARS-CoV-2

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-07-01 DOI: 10.1016/j.virs.2025.06.006

Zixiao Yang, Xinrong Zhou, Xikui Sun, Liu Cao, Tiefeng Xu, Kun Li, Hongchao Liu, Yanxi Ji, Lihong Liu, Konstantin I Ivanov, Zhonghan Yang, Deyin Guo, Chun-Mei Li

{"title":"Rapid and accurate detection of infectious SARS-CoV-2 by viral receptor capture combined with loop-mediated isothermal amplification.","authors":"Zixiao Yang, Xinrong Zhou, Xikui Sun, Liu Cao, Tiefeng Xu, Kun Li, Hongchao Liu, Yanxi Ji, Lihong Liu, Konstantin I Ivanov, Zhonghan Yang, Deyin Guo, Chun-Mei Li","doi":"10.1016/j.virs.2025.06.006","DOIUrl":"https://doi.org/10.1016/j.virs.2025.06.006","url":null,"abstract":"Rapid and accurate detection of infectious virus particles, not just viral nucleic acid, is essential to avoid unnecessary quarantine and effectively control the spread of viral diseases such as coronavirus disease 2019 (COVID-19), severe acute respiratory syndrome (SARS), and Middle East respiratory syndrome (MERS). Real-time quantitative polymerase chain reaction (RT-qPCR) was the most widely used detection technique during the COVID-19 outbreak. However, it cannot discriminate between intact infectious viruses and surface-distorted, non-infectious virus particles or naked viral RNA. In this study, we present a strategy for the specific detection of infectious coronaviruses by combining viral receptor capture and reverse transcription loop-mediated isothermal amplification (RT-LAMP). We successfully applied this strategy to detect infectious virus particles of the SARS-CoV-2 surrogate virus and the human coronavirus NL63 (HCoV-NL63). Virus particles were first captured on ELISA plates coated with the recombinant human angiotensin-converting enzyme 2 (hACE2) receptor. Viral RNA was then extracted from the particles and detected by RT-LAMP using virus-specific primers. In our experimental setting, the proposed method had a minimum detection limit (LOD) of 90 PFU/mL, sensitivity of 96.2%, and specificity of 100%. Our study provides a proof-of-concept that viral receptor capture combined with RT-LAMP can differentiate infectious coronaviruses from non-infectious virions or naked viral RNA. This paves the way for this virus detection strategy to become a mainstream tool for the management, prevention and control of epidemic coronavirus diseases.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virome diversity in small mammals from south China: insights into virus evolution, transmission, and ecology. 华南小型哺乳动物的病毒群多样性：对病毒进化、传播和生态学的见解。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-27 DOI: 10.1016/j.virs.2025.06.004

Yiwei Shi, Letian Fang, Cixiu Li, Peng Li, Jiluo Liu, Yifan Chen, Yue Zhao, Zishuai Li, Shuqi Liu, Yibo Ding, Xinyu Zhou, Dongming Jiang, Jiaying Shen, Zihan Zhang, Junheng Lyu, Rui Pu, Xiaojie Tan, Jianhua Yin, Weifeng Shi, Guangwen Cao

{"title":"Virome diversity in small mammals from south China: insights into virus evolution, transmission, and ecology.","authors":"Yiwei Shi, Letian Fang, Cixiu Li, Peng Li, Jiluo Liu, Yifan Chen, Yue Zhao, Zishuai Li, Shuqi Liu, Yibo Ding, Xinyu Zhou, Dongming Jiang, Jiaying Shen, Zihan Zhang, Junheng Lyu, Rui Pu, Xiaojie Tan, Jianhua Yin, Weifeng Shi, Guangwen Cao","doi":"10.1016/j.virs.2025.06.004","DOIUrl":"https://doi.org/10.1016/j.virs.2025.06.004","url":null,"abstract":"Mammals are critical reservoirs of human infectious diseases and the spillover of viruses is related to climate conditions. We conducted meta-transcriptomic sequencing of 226 mammals (bats, rodents, hedgehogs, and shrews) representing 20 species collected across eight cities in south China between 2018 and 2024. Samples included internal organs, oropharyngeal and anal swabs, and feces. We identified 63 vertebrate-associated viruses, including 34 novel viruses. Phylogenetic analysis revealed six viruses with potential infection risks to humans or domestic animals due to their close phylogenetic relationships with known pathogens. Cross-species transmission was observed in 14.3% (9/63) of viruses, shared by at least two host species, with bats, particularly Rhinolophus and Hipposideros, serving as key hubs for viral circulation and zoonotic spillover. Virome composition varied substantially among mammalian species and geographic regions (adonis R2 = 0.50, P = 0.001). Generalized linear models quantified the roles of host taxonomy, ecotypes, and meteorological factors in shaping viral diversity, demonstrating host taxonomy (at the order level) as a predominant role (25.70% deviance explained), followed by ecotypes (10.27% deviance explained). Phylogenetic analysis conducted using our betacoronavirus sequences, as well as betacoronavirus sequences derived from 2.0 × 104 bats sampled in China between July 2013 and March 2024, revealed that no betacoronaviruses exhibited closer phylogenetic relationships to SARS-CoV-2 than the known strains (e.g., RaTG13). These findings provide critical insights into virus evolution, transmission, and ecological determinants, which are essential for the prevention of emerging infectious diseases.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0