Virologica Sinica最新文献_第2页

Elevated interferon-induced transmembrane protein 3 in platelets and megakaryocytes suppresses Crimean-Congo hemorrhagic fever viral infection by interacting with glycoprotein Gc. 血小板和巨核细胞中干扰素诱导的跨膜蛋白3升高通过与糖蛋白Gc相互作用抑制克里米亚-刚果出血热病毒感染。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-05-21 DOI: 10.1016/j.virs.2025.05.002

Jingyuan Zhang, Yaohui Fang, Chenhui Lin, Xiaoli Wu, Chaoxiong Yue, Fei Deng, Shu Shen

{"title":"Elevated interferon-induced transmembrane protein 3 in platelets and megakaryocytes suppresses Crimean-Congo hemorrhagic fever viral infection by interacting with glycoprotein Gc.","authors":"Jingyuan Zhang, Yaohui Fang, Chenhui Lin, Xiaoli Wu, Chaoxiong Yue, Fei Deng, Shu Shen","doi":"10.1016/j.virs.2025.05.002","DOIUrl":"10.1016/j.virs.2025.05.002","url":null,"abstract":"Crimean-Congo hemorrhagic fever (CCHF) is a hemorrhagic fever caused by infection with the CCHF virus (CCHFV) and has a mortality rate of up to 30 %. Thrombocytopenia is a hallmark of CCHF; however, the mechanisms underlying this manifestation remain poorly understood. In addition to hemostasis, platelets play a crucial role in recognizing pathogens and mediating immune responses. We investigated the mechanisms underlying thrombocytopenia associated with CCHFV infection by analyzing the platelet transcriptome in mice. Interferon-induced transmembrane protein 3 (IFITM3), a known antiviral factor, was significantly upregulated. The role of IFITM3 in response to CCHFV infection was characterized using the human megakaryoblast cell line MEG-01, considered a parental cell line of platelets. Although the CCHFV infection rate was limited, MEG-01 cells maintained the infection and replication of CCHFV, leading to increased IFITM3 protein expression. We demonstrated that IFITM3 overexpression efficiently inhibited CCHFV infection, whereas IFITM3 knockout promoted viral infection. An interaction between IFITM3 and the CCHFV glycoprotein Gc was identified, which suppressed CCHFV entry into cells. The IFITM3 CIL-TMD domain is critical for this interaction. These results suggest that IFITM3 is a restriction factor and plays an antiviral role during CCHFV infection. Elevated expression of IFITM3 in platelets indicates that this could be a common mechanism by which platelets protect against viruses, including CCHFV, which may reduce platelet consumption and destruction caused by CCHFV infection. These findings provide valuable insights into the pathogenesis of CCHF-associated thrombocytopenia and offer foundational theoretical support for future therapeutic strategies.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutational landscapes of NITD008-resistant EV71 variants revealed through population sequencing. 通过群体测序揭示了nitd008抗性EV71变异的突变景观。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-05-21 DOI: 10.1016/j.virs.2025.05.003

Fang Yu, Qiu-Yan Zhang, Zhe-Rui Zhang, Cheng-Lin Deng, Bo Zhang

引用次数: 0

Characterization of a SARS-CoV-2 infection model in golden hamsters with diabetes mellitus. 糖尿病金仓鼠SARS-CoV-2感染模型的建立

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-05-17 DOI: 10.1016/j.virs.2025.05.001

Hao-Feng Lin, Ren-Di Jiang, Rui-Xin Qin, Bing Yao, Wen-Tao Zeng, Yun Gao, Ai-Min Shi, Jian-Min Li, Mei-Qin Liu

{"title":"Characterization of a SARS-CoV-2 infection model in golden hamsters with diabetes mellitus.","authors":"Hao-Feng Lin, Ren-Di Jiang, Rui-Xin Qin, Bing Yao, Wen-Tao Zeng, Yun Gao, Ai-Min Shi, Jian-Min Li, Mei-Qin Liu","doi":"10.1016/j.virs.2025.05.001","DOIUrl":"10.1016/j.virs.2025.05.001","url":null,"abstract":"Being widespread across the globe, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) keeps evolving and generating new variants and continuously poses threat to public health, especially to the population with chronic comorbidities. Diabetes mellitus is one of high-risk factors for severe outcome of coronavirus disease 2019 (COVID-19). Establishment of animal models that parallel the clinical and pathological features of COVID-19 complicated with diabetes is thus highly essential. Here, in this study, we constructed leptin receptor gene knockout hamsters with the phenotype of diabetes mellitus (db/db), and revealed that the diabetic hamsters were more susceptible to SARS-CoV-2 and its variants than wild-type hamsters. SARS-CoV-2 and its variants induced a stronger immune cytokine response in the lungs of diabetic hamsters than in wild-type hamsters. Comparative histopathology analyses also showed that infection of SARS-CoV-2 and the variants caused more severe lung tissue injury in diabetic hamsters, and may induce serious complications such as diabetic kidney disease and cardiac lesions. Our findings demonstrated that despite the decreased respiratory pathogenicity, the SARS-CoV-2 variants were still capable of impairing other organs such as kidney and heart in diabetic hamsters, suggesting that the risk of evolving SARS-CoV-2 variants to diabetic patients should never be neglected. This hamster model may help better understand the pathogenesis mechanism of severe COVID-19 in patients with diabetes. It will also aid in development and testing of effective therapeutics and prophylactic treatments against SARS-CoV-2 variants among these high-risk populations.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mouse model of Crimean-Congo hemorrhagic fever virus-induced coagulopathy. 克里米亚-刚果出血热病毒致凝血病小鼠模型。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-30 DOI: 10.1016/j.virs.2025.04.008

Hui Zhang, Ziyang Jiang, Haidang Liao, Jiang Li, Manli Wang, Yiwu Zhou, Zhihong Hu, Jia Liu

引用次数: 0

A noninfectious pseudovirus system for an emerging orthoflavivirus. 新出现的正黄病毒的非传染性假病毒系统。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-23 DOI: 10.1016/j.virs.2025.04.006

Yanfan Shi, Yu He, Xiaoli Wang, Zhen Wu, Tao Wang, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Di Sun, Anchun Cheng, Shun Chen

引用次数: 0

Viral pseudo-enzyme facilitates KSHV lytic replication via suppressing PFAS-mediated RTA deamidation. 病毒伪酶通过抑制pfas介导的RTA脱酰胺促进KSHV裂解复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-12 DOI: 10.1016/j.virs.2025.04.005

Yang Xu, Qiushi Zhang, Guoli Hou, Liang Hu, Tiaoyi Xiao, Xinyu Liang, Deliang Li, Junhua Li

{"title":"Viral pseudo-enzyme facilitates KSHV lytic replication via suppressing PFAS-mediated RTA deamidation.","authors":"Yang Xu, Qiushi Zhang, Guoli Hou, Liang Hu, Tiaoyi Xiao, Xinyu Liang, Deliang Li, Junhua Li","doi":"10.1016/j.virs.2025.04.005","DOIUrl":"10.1016/j.virs.2025.04.005","url":null,"abstract":"Deamidation, a type of post-translational modification commonly considered a hallmark of protein \"aging\" and function decay, is increasingly recognized for its pivotal role in regulating biological processes and viral infection. Our previous study has demonstrated that the deamidation of replication and transcription activator (RTA), a master regulator of ubiquitous and oncogenic Kaposi's sarcoma-associated herpesvirus (KSHV), mediated by phosphoribosylformylglycinamidine synthetase (PFAS), hinders its nuclear import and transcriptional activity. Here we report that the viral glutamine amidotransferase (vGAT) pseudo-enzyme is exploited to facilitate KSHV lytic infection by inhibiting RTA deamidation. To be more specific, vGAT interacts with both RTA and cellular PFAS, and inhibits PFAS-mediated RTA deamidation, thus facilitating RTA nuclear localization and suppressing nuclear factor-kappa B (NF-κB) signaling activation, as well as augmenting RTA-mediated transcriptional activation of viral open reading frames (ORFs). In addition, vGAT appears to regulate the deamidation process of several viral ORFs of KSHV. Collectively, these findings unveil that a viral pseudo-enzyme is exploited to enhance viral infection via deamidation regulation.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scutellaria barbata D. Don extracts alleviate SARS-CoV-2 induced acute lung injury by inhibiting virus replication and bi-directional immune modulation. 黄芩提取物通过抑制病毒复制和双向免疫调节减轻SARS-CoV-2诱导的急性肺损伤。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-12 DOI: 10.1016/j.virs.2025.04.004

Yan Ran, Zinuo Chen, Carolina Q Sacramento, Lingyuan Fan, Qinghua Cui, Lijun Rong, Ruikun Du

{"title":"Scutellaria barbata D. Don extracts alleviate SARS-CoV-2 induced acute lung injury by inhibiting virus replication and bi-directional immune modulation.","authors":"Yan Ran, Zinuo Chen, Carolina Q Sacramento, Lingyuan Fan, Qinghua Cui, Lijun Rong, Ruikun Du","doi":"10.1016/j.virs.2025.04.004","DOIUrl":"10.1016/j.virs.2025.04.004","url":null,"abstract":"The emergence of SARS-CoV-2 variants and drug-resistant mutants emphasizes the urgent need to develop novel antiviral agents. In the present study, we examined the therapeutic effect of the Chinese medicinal herb, Scutellaria barbata D. Don (SBD), against SARS-CoV-2 infection both in vitro and in vivo. Using a viral replicon particle (VRP)-based mouse model of SARS-CoV-2 infection, our study revealed that SBD extracts can reduce viral load in mouse lungs and alleviate the viral induced pneumonia. In vitro antiviral determination further validated the direct acting antiviral efficacy of SBD extracts against SARS-CoV-2 replication. Mechanistic studies demonstrated that SBD can act against SARS-CoV-2 replication by targeting both 3-chymotrypsin-like and papain-like cysteine proteases, via a combination of multiple active constituents. Moreover, SBD can modulate the host inflammation response in a bi-directional manner, which also contribute to the mitigation of viral induced acute lung injury. In summary, our study provides SBD as a promising therapeutic agent to combat SARS-CoV-2 infections that merit further development.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coadministration of ribavirin and arenaviral entry inhibitor LHF-535 enhances antiviral benefit against authentic lassa virus. 利巴韦林和沙粒病毒进入抑制剂LHF-535联合使用可增强对真正拉沙病毒的抗病毒作用。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-12 DOI: 10.1016/j.virs.2025.04.003

Cheng Peng, Jialing Hu, Yuan Bai, Wei Wu, Wenting Mao, Yang Liu, Yi Wan, Lei Zhang, Wei Li, Tingting Tian, Tiezhu Liu, Yanhai Wang, Mifang Liang, Jun Han, Zhiming Yuan, Jiandong Li, Chao Shan, Fei Deng, Wei Wang

引用次数: 0

Differential susceptibility of immunodeficient mice to MPXV infection and the impact of various inoculation routes. 免疫缺陷小鼠对MPXV感染的差异易感性及不同接种途径的影响。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-07 DOI: 10.1016/j.virs.2025.04.001

Xiaohan Wang, Shaowen Shi, Xiaoxuan Nie, Yongyang Sun, Jinglei Hu, Manlin He, Wenhao Ren, Yuxing Wang, Zhendong Guo, Gonghe Li, Changbo Ou, Xiao Li, Zongzheng Zhao

{"title":"Differential susceptibility of immunodeficient mice to MPXV infection and the impact of various inoculation routes.","authors":"Xiaohan Wang, Shaowen Shi, Xiaoxuan Nie, Yongyang Sun, Jinglei Hu, Manlin He, Wenhao Ren, Yuxing Wang, Zhendong Guo, Gonghe Li, Changbo Ou, Xiao Li, Zongzheng Zhao","doi":"10.1016/j.virs.2025.04.001","DOIUrl":"https://doi.org/10.1016/j.virs.2025.04.001","url":null,"abstract":"Monkeypox virus (MPXV), a member of the Orthopoxvirus genus, caused a large-scale global outbreak in 2022. Developing mouse models for MPXV infection is crucial for advancing research on vaccines and therapeutic interventions. To address this, we conducted a comparative study on the susceptibility of six mouse strains-severe combined immune-deficiency (SCID), nude, genetically diabetic (db/db) and obese (ob/ob), C57BL/6J, and BALB/c-to MPXV infection. Mouse strains were infected with MPXV via intranasal inoculation, and body weight changes and mortality were monitored post-infection. Additionally, the tissue distribution of MPXV and the pathological changes in the lung tissues of the infected mice were evaluated. The results demonstrated that SCID and nude mice exhibited significant weight loss following MPXV infection, with 100 % mortality observed in SCID mice, while no mortality occurred in nude mice. In contrast, the other mouse strains showed no significant weight loss or mortality. Notably, the viral load in the lung tissues of SCID and nude mice was the highest among the tested strains. Furthermore, we investigated the impact of different inoculation routes-intranasal (I.N.), intraperitoneal (I.P.), and intravenous (I.V.)-on the pathogenicity of MPXV in mice. The results revealed that the intravenous route induced more pronounced pathogenic effects compared to the intranasal and intraperitoneal routes. In summary, this study provides valuable insights into the development of MPXV-infected mouse models, offering a foundation for further research on MPXV pathogenesis and therapeutic drug development.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WITHDRAWN: Comparative metatranscriptome analysis in gut reveals insignificant host or microbiota changes in SARS-related coronavirus naturally infected bats. 撤回：肠道的比较转录组分析显示，在sars相关冠状病毒自然感染的蝙蝠中，宿主或微生物群的变化微不足道。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-07 DOI: 10.1016/j.virs.2025.04.002

Qi Wang, Shi-Qiang Mei, Tian-Yi Dong, Jia Su, Yuan-Fei Pan, Yan Zhu, Ke Wu, Li-Biao Zhang, Mang Shi, Peng Zhou

引用次数: 0