Virologica Sinica最新文献_第2页

Virome diversity in small mammals from south China: insights into virus evolution, transmission, and ecology. 华南小型哺乳动物的病毒群多样性：对病毒进化、传播和生态学的见解。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-27 DOI: 10.1016/j.virs.2025.06.004

Yiwei Shi, Letian Fang, Cixiu Li, Peng Li, Jiluo Liu, Yifan Chen, Yue Zhao, Zishuai Li, Shuqi Liu, Yibo Ding, Xinyu Zhou, Dongming Jiang, Jiaying Shen, Zihan Zhang, Junheng Lyu, Rui Pu, Xiaojie Tan, Jianhua Yin, Weifeng Shi, Guangwen Cao

{"title":"Virome diversity in small mammals from south China: insights into virus evolution, transmission, and ecology.","authors":"Yiwei Shi, Letian Fang, Cixiu Li, Peng Li, Jiluo Liu, Yifan Chen, Yue Zhao, Zishuai Li, Shuqi Liu, Yibo Ding, Xinyu Zhou, Dongming Jiang, Jiaying Shen, Zihan Zhang, Junheng Lyu, Rui Pu, Xiaojie Tan, Jianhua Yin, Weifeng Shi, Guangwen Cao","doi":"10.1016/j.virs.2025.06.004","DOIUrl":"https://doi.org/10.1016/j.virs.2025.06.004","url":null,"abstract":"Mammals are critical reservoirs of human infectious diseases and the spillover of viruses is related to climate conditions. We conducted meta-transcriptomic sequencing of 226 mammals (bats, rodents, hedgehogs, and shrews) representing 20 species collected across eight cities in south China between 2018 and 2024. Samples included internal organs, oropharyngeal and anal swabs, and feces. We identified 63 vertebrate-associated viruses, including 34 novel viruses. Phylogenetic analysis revealed six viruses with potential infection risks to humans or domestic animals due to their close phylogenetic relationships with known pathogens. Cross-species transmission was observed in 14.3% (9/63) of viruses, shared by at least two host species, with bats, particularly Rhinolophus and Hipposideros, serving as key hubs for viral circulation and zoonotic spillover. Virome composition varied substantially among mammalian species and geographic regions (adonis R2 = 0.50, P = 0.001). Generalized linear models quantified the roles of host taxonomy, ecotypes, and meteorological factors in shaping viral diversity, demonstrating host taxonomy (at the order level) as a predominant role (25.70% deviance explained), followed by ecotypes (10.27% deviance explained). Phylogenetic analysis conducted using our betacoronavirus sequences, as well as betacoronavirus sequences derived from 2.0 × 104 bats sampled in China between July 2013 and March 2024, revealed that no betacoronaviruses exhibited closer phylogenetic relationships to SARS-CoV-2 than the known strains (e.g., RaTG13). These findings provide critical insights into virus evolution, transmission, and ecological determinants, which are essential for the prevention of emerging infectious diseases.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of a newly discovered broad-spectrum Acinetobacter baumannii phage and therapeutic validation against pan-resistant Acinetobacter baumannii. 新发现的广谱鲍曼不动杆菌噬菌体的分离鉴定及对泛耐药鲍曼不动杆菌的治疗效果验证。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-27 DOI: 10.1016/j.virs.2025.06.003

Miaomiao Lin, Lele Xiong, Wen Li, Lingyan Xiao, Wei Zhang, Xiaogui Zhao, Yishan Zheng

{"title":"Isolation and identification of a newly discovered broad-spectrum Acinetobacter baumannii phage and therapeutic validation against pan-resistant Acinetobacter baumannii.","authors":"Miaomiao Lin, Lele Xiong, Wen Li, Lingyan Xiao, Wei Zhang, Xiaogui Zhao, Yishan Zheng","doi":"10.1016/j.virs.2025.06.003","DOIUrl":"https://doi.org/10.1016/j.virs.2025.06.003","url":null,"abstract":"The treatment of Acinetobacter baumannii (A. baumannii) poses significant clinical challenges due to its multidrug/pan-drug resistance. In this study, we isolated a broad-spectrum lytic A. baumannii phage, named P425, from medical wastewater, targeting nine multidrug-resistant A. baumannii (MDRAB) with diverse capsular types. Biological characterization revealed that P425 maintains activity at pH range of 3-12 and temperature range of 4-50 °C. It resists UV irradiation for 20 minutes, and had an optimal multiplicity of infection (MOI) is 0.00001. The adsorption kinetics showed that P425 achieves > 90% within 10 minutes of incubation, and the one-step growth curve indicated a 10-minute latent period, with a burst size of 184 PFU/cell. The genome sequencing results indicated that it harbors a double-stranded DNA genome of 40,583 bp with a GC content of 39.39%. Intergenomic similarity analysis classified it as a novel species within the Friunavirus genus, while electron microscopy results showed that it belongs to the Podoviridae family. Notably, P425 exhibits potent 24-hour in vitro inhibitory activity against MDRAB, and demonstrates synergistic effect at an MOI of 0.001 when combined with five classes of antibiotics targeting distinct antimicrobial mechanisms. Safety evaluations confirmed the absence of cytotoxicity, hemolytic activity, or systemic toxicity both in vitro and in vivo. In mouse infection models, P425 can significantly improve the survival rates of mice infected with Ab25 (ST1791/KL101). When co-administered with levofloxacin, it can achieved 100% protection against mortality and promoted immunological recovery. Collectively, P425 is a prospective lytic phage that could offer novel strategies for combating MDRAB infections.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First isolation and genomic characterization of a novel inter-genotype recombinant feline calicivirus from domestic cats: implications for viral evolution. 首次从家猫中分离出一种新的跨基因型重组猫杯状病毒并进行基因组鉴定：对病毒进化的影响。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-27 DOI: 10.1016/j.virs.2025.06.005

Xiaomei Tan, Liangliang Lin, Qi Zhang, Na Li, Ningning Dong, Shishi Wang, Wenqi Zhu, Maoqing Luo, Shuaisai Pang, Yanzhao Xu, Guangqing Liu, Chunchun Meng

引用次数: 0

Identification of PEDV inhibitors targeting 3CL protease. 靶向3CL蛋白酶的PEDV抑制剂的鉴定。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-27 DOI: 10.1016/j.virs.2025.06.002

Ang Tian, Shutong Shi, Siying Zou, Shuaiyin Guan, Hao Wu, Zhen Li, Huanchun Chen, Yunfeng Song

{"title":"Identification of PEDV inhibitors targeting 3CL protease.","authors":"Ang Tian, Shutong Shi, Siying Zou, Shuaiyin Guan, Hao Wu, Zhen Li, Huanchun Chen, Yunfeng Song","doi":"10.1016/j.virs.2025.06.002","DOIUrl":"https://doi.org/10.1016/j.virs.2025.06.002","url":null,"abstract":"Porcine epidemic diarrhea (PED), caused by porcine epidemic diarrhea virus (PEDV), is a highly contagious gastrointestinal disease characterized by vomiting, diarrhea, and dehydration, with mortality rates approaching 100% among suckling piglets. The PEDV 3C-like protease (3CLpro) is essential for viral replication and regarded as a critical target for antiviral inhibitor development. In this study, we aimed to identify small-molecule inhibitors of PEDV by targeting 3CLpro. Virtual screening of 1.6 million compounds from the ChemDiv library identified four potential candidates. Molecular dynamics simulations, (RMSD, RMSF, Rg) revealed enhanced structural stability of the compound-protease complexes compared to the monomeric enzyme. All compounds had low cytotoxicity in Vero cells (CC50 > 200 μM). Fluorescence resonance energy transfer-based assays demonstrated dose-dependent inhibitory activity of the compounds against 3CLpro. Among the candidates, compound F366-0161 exhibited the weakest inhibition, with an IC50 value of 151.5 μM. Two analogues, 3238-0395 (IC50 of 121.4 μM) and L878-0493 (IC50 of 123.6 μM), exhibited moderately enhanced activity. Y041-1672 was identified as the most effective inhibitor, with an IC50 of 86.48 μM. In viral replication inhibition assays, Y041-1672 reduced PEDV replication, with an EC50 of 17.97 μM and a selectivity index (SI) of 15.5 (CC50/EC50). These results were validated by RT-qPCR, plaque assays, immunofluorescence, and Western blot analyses. In vitro validation confirmed Y041-1672 as the optimal antiviral candidate, and time-of-addition experiments indicated that inhibition primarily occurred during viral replication. This study identifies scaffold molecules for PEDV antiviral drug development, providing strategic insights for PED treatment.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Evaluating the performance of the PREDAC method in flu vaccine recommendations over the past decade (2013-2023)" [Virol. Sin. 40 (2025) 288-291]. “评估过去十年（2013-2023）PREDAC方法在流感疫苗推荐中的表现”的勘误表性研究。罪恶，40 (2025)288-291 [j]。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-21 DOI: 10.1016/j.virs.2025.06.001

Yousong Peng, Lei Yang, Weijuan Huang, Mi Liu, Xiao Ding, Xiangjun Du, Yuelong Shu, Taijiao Jiang, Dayan Wang

引用次数: 0

Nucleophosmin 1 inhibits the replication of influenza A virus by competitively binding viral RNA with viral proteins. 核磷蛋白1通过竞争性结合病毒RNA与病毒蛋白抑制甲型流感病毒的复制。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-05 DOI: 10.1016/j.virs.2025.04.007

Yingying Yu, Qian Wang, Yanli Wei, Junwen Liu, Guangwen Wang, Zhengxiang Wang, Wentao Shen, Lu Han, Chengjun Li, Cao-Qi Lei, Shuai Xu, Qiyun Zhu

引用次数: 0

The slow progression of Japanese encephalitis in aged mice is likely associated to B cell recruitment in the brain. 老年小鼠乙型脑炎的缓慢进展可能与脑内B细胞募集有关。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-03 DOI: 10.1016/j.virs.2025.05.013

Zheng-Ran Song, Yi-Lin Yang, Yang Zhou, Li-Bo Liu, Fei-Yang Xue, Lin-Shen-Yang Liu, Na Gao, Dong-Ying Fan, Yi-Song Wang, Jing An, Pei-Gang Wang

{"title":"The slow progression of Japanese encephalitis in aged mice is likely associated to B cell recruitment in the brain.","authors":"Zheng-Ran Song, Yi-Lin Yang, Yang Zhou, Li-Bo Liu, Fei-Yang Xue, Lin-Shen-Yang Liu, Na Gao, Dong-Ying Fan, Yi-Song Wang, Jing An, Pei-Gang Wang","doi":"10.1016/j.virs.2025.05.013","DOIUrl":"10.1016/j.virs.2025.05.013","url":null,"abstract":"The Japanese encephalitis virus (JEV) causes Japanese encephalitis (JE), a severe disease that primarily affects children and induces significant central nervous system complications. With the widespread adoption of vaccination in children, the incidence among older individuals has increased substantially. Despite this epidemiological shift, research on JEV infection in the elderly remains limited. We established JEV infection models using both aged and young mice to explore age-related differences in pathology and underlying mechanisms. Brain tissue samples were analyzed for pathological changes and viral tropism in major cell types. To further characterize immune response variations, we conducted transcriptomic sequencing on the brain tissues following JEV infection. Aged mice exhibited lower mortality, delayed disease progression, and milder brain pathology compared to young mice after JEV infection. Viral titers and infection rates of major brain cell types were similar in both groups. Transcriptomic analysis revealed diminished immune activation and weaker inflammatory responses in aged mice. Additionally, microglial activation and CD8+ T cell function were significantly reduced. Interestingly, JEV infection induced the selective recruitment of B cells in the brains of aged mice. These B cells may modulate the effects of CD8+ T cells in the disease process. Compared to young mice, aged mice showed enhanced resistance to JEV progression and reduced brain pathology. This resistance was associated with a weakened immune response in the aged brain, rather than differences in viral infection. The specific recruitment of B cells in the brains of aged mice may play a crucial role in limiting disease progression.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the virome reveals diverse novel viruses in tree shrews (Tupaia belangeri) in Yunnan Province. 解码病毒组揭示了云南省树鼩（图帕亚belangeri）中多种新型病毒。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-01 DOI: 10.1016/j.virs.2025.05.011

Zhong-Hao Lian, Zhi You, Pei-Yu Han, Ye Qiu, Yun-Zhi Zhang, Xing-Yi Ge

{"title":"Decoding the virome reveals diverse novel viruses in tree shrews (Tupaia belangeri) in Yunnan Province.","authors":"Zhong-Hao Lian, Zhi You, Pei-Yu Han, Ye Qiu, Yun-Zhi Zhang, Xing-Yi Ge","doi":"10.1016/j.virs.2025.05.011","DOIUrl":"10.1016/j.virs.2025.05.011","url":null,"abstract":"Viruses circulating in small mammals possess the potential to infect humans. Tree shrews are a group of small mammals inhabiting widely in forests and plantations, but studies on viruses in tree shrews are quite limited. Herein, viral metagenomic sequencing was employed to detect the virome in the tissue and swab samples from seventy-six tree shrews that we collected in Yunnan Province. As the results, genomic fragments belonging to eighteen viral families were identified, thirteen of which contain mammalian viruses. Through polymerase chain reaction (PCR) and Sanger sequencing, twelve complete genomes were determined, including five parvoviruses, three torque teno viruses (TTVs), two adenoviruses, one pneumovirus, and one hepacivirus, together with three partial genomes, including two hepatitis E viruses and one paramyxovirus. Notably, the three TTVs, named TSTTV-HNU1, TSTTV-HNU2, and TSTTV-HNU3, may compose a new genus within the family Anelloviridae. Notably, TSParvoV-HNU5, one of the tree shrew parvoviruses detected, was likely to be a recombination of two murine viruses. Divergence time estimation further revealed the potential cross-species-transmission history of the tree shrew pneumovirus TSPneV-HNU1. Our study provides a comprehensive exploration of viral diversity in wild tree shrews, significantly enhancing our understanding of their roles as natural virus reservoirs.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clofazimine targeting the spike protein and RdRp exhibits highly efficient antiviral activity against porcine epidemic diarrhea virus in vitro. 氯法齐明靶向刺突蛋白和RdRp对猪流行性腹泻病毒表现出高效的体外抗病毒活性。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-06-01 DOI: 10.1016/j.virs.2025.05.012

Shuting Zhou, Junrui Zhu, Houde Zhao, Zixin Huang, Kangqi Zheng, Fan Xia, Yufan Xu, Guocheng Zhao, Jijie Jiang, En Zhang, Haoyang Nian, Li Cui, Tao Sun, Xiangfeng Wang, Yanjun Zhou, Zhibiao Yang, Zhe Wang

{"title":"Clofazimine targeting the spike protein and RdRp exhibits highly efficient antiviral activity against porcine epidemic diarrhea virus in vitro.","authors":"Shuting Zhou, Junrui Zhu, Houde Zhao, Zixin Huang, Kangqi Zheng, Fan Xia, Yufan Xu, Guocheng Zhao, Jijie Jiang, En Zhang, Haoyang Nian, Li Cui, Tao Sun, Xiangfeng Wang, Yanjun Zhou, Zhibiao Yang, Zhe Wang","doi":"10.1016/j.virs.2025.05.012","DOIUrl":"10.1016/j.virs.2025.05.012","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) infection causes acute watery diarrhea in neonatal piglets, leading to substantial economic losses within the pig farming industry. This study demonstrates that clofazimine (CFZ) significantly inhibits PEDV replication in a dose-dependent manner in vitro, with negligible cytotoxicity. Findings from our time-of-addition assays indicate that CFZ effectively disrupts multiple stages of the viral infection cycle. Using a CoV-RdRp-Gluc reporter system, we evaluated the potency of CFZ against PEDV RNA-dependent RNA polymerase (RdRp), and determined a low IC50 value of 0.1364 μM. Molecular docking studies further confirmed that CFZ has high binding affinity at the active sites of the spike protein and RdRp protein in PEDV. Transcriptome analysis of Vero E6 cells, with and without CFZ treatment, revealed a significant change in transcriptional activity at 8 h post-infection (hpi). Moreover, the simultaneous application of CFZ and nucleoside analogs showed enhanced the anti-PEDV effect of CFZ in vitro. Our study underscores the potential of CFZ as a viable therapeutic agent against PEDV.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Severe enterovirus A71 infection is associated with dysfunction of T cell immune response and alleviated by Astragaloside A. 重度肠病毒A71感染与T细胞免疫应答功能障碍相关，黄芪甲苷A可减轻其免疫功能障碍。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-05-29 DOI: 10.1016/j.virs.2025.05.010

Chong Wang, Muhan Huang, Bingyu Guo, Xi Zhou, Zongqiang Cui, Yi Xu, Yujie Ren

{"title":"Severe enterovirus A71 infection is associated with dysfunction of T cell immune response and alleviated by Astragaloside A.","authors":"Chong Wang, Muhan Huang, Bingyu Guo, Xi Zhou, Zongqiang Cui, Yi Xu, Yujie Ren","doi":"10.1016/j.virs.2025.05.010","DOIUrl":"10.1016/j.virs.2025.05.010","url":null,"abstract":"Enterovirus A71 (EV-A71) is the major causative pathogen for severe hand-foot-mouth disease (HFMD), a predominantly childhood-associated communicable disease. The mechanisms that children manifest severe disease progression while adults typically exhibit milder or asymptomatic infections remain incompletely characterized, which hinders the development of effective therapy against this disease. Herein, using the newborn mouse model of EV-A71 infection, we uncovered that the underdevelopment of T cells closely associated with the severity of EV-A71 infection, and EV-A71 infection dramatically impaired T-cell immune response. Moreover, the dysfunction of T-cell immunity contributes to the pathogenesis of EV-A71 infection, as the loss of T cells made neonatal mice highly vulnerable to EV-A71 infection. To further assess the relationship between T-cell immunity and HFMD, we enrolled a cohort of 145 pediatric patients with laboratory-confirmed EV-A71 infection and found that the compromised T-cell immune response is associated with the severity of EV-A71-caused HFMD in these children. Furthermore, we found that the treatment of newborn mice with Astragaloside A, a saponin from the medicinal herb Astragalus membranaceus, showed potent in vivo therapeutic efficacy against EV-A71 infection in a T-cell-dependent manner. In conclusion, these findings uncover the interaction between EV-A71 infection and T-cell immunity, provide novel insights onto the physiological impacts of T cells on the pathogenesis of EV-A71 infection and HFMD, and find a promising immunotherapeutic strategy to treat this viral disease.","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0