Virologica SinicaPub Date : 2025-04-01DOI: 10.1016/j.virs.2025.03.005
Liangyu Li , Haiyue Zhang , Pei Xiong , Chan Liu , Lu Wan , Mengling Liu , Jieyu Mao , Ruiyun Li , Min Shang , Hailing Liu , Yuchuan Luo , Jing Yin , Xiaojun Wu , Jianjun Chen
{"title":"Virus profiling of bronchoalveolar lavage fluid in hospitalized non-COVID-19 adult patients with pulmonary infection from November 2020 to November 2021","authors":"Liangyu Li , Haiyue Zhang , Pei Xiong , Chan Liu , Lu Wan , Mengling Liu , Jieyu Mao , Ruiyun Li , Min Shang , Hailing Liu , Yuchuan Luo , Jing Yin , Xiaojun Wu , Jianjun Chen","doi":"10.1016/j.virs.2025.03.005","DOIUrl":"10.1016/j.virs.2025.03.005","url":null,"abstract":"<div><div>Identifying the cause of respiratory tract infections is important for reducing the burden of diagnosis and treatment. To assess viral etiologies of hospitalized patients with pulmonary infection, bronchoalveolar lavage fluid (BALF) specimens were collected from non-COVID-19 adult patients (n = 333, including patients with lower respiratory tract infection, tuberculosis, lung cancer, and pulmonary nodules) between November 2020 and November 2021. Multiple common respiratory pathogens were detected using multiplex reverse-transcription polymerase chain reaction. The result showed that at least one virus was identified in 35.44% (118/333) of the cases. Among these, influenza virus was the most commonly identified, followed by the parainfluenza virus, coronavirus, human rhinoviruses, and human respiratory syncytial viruses. The tuberculosis group demonstrated the highest viral detection rate, yet paradoxically exhibited the lowest co-infection rate. In contrast, the highest co-infection frequency was observed in the pulmonary nodules group. Patients with viral infections exhibited more severe clinical symptoms compared to those without detected viral infections. However, this observation was only noted in the lower respiratory tract infection group among the different disease groups. Notably, among patients infected with a specific virus, there were no significant differences in viral load between single and co-infections. Our study identified the major causative agents in hospitalized adult patients with pulmonary infection, offering insights for precise disease diagnosis and the prevention of unnecessary use of antimicrobial drugs.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 166-175"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Virologica SinicaPub Date : 2025-04-01DOI: 10.1016/j.virs.2025.01.003
Ziheng Feng , Zhengde Xie , Lili Xu
{"title":"Current antiviral therapies and promising drug candidates against respiratory syncytial virus infection","authors":"Ziheng Feng , Zhengde Xie , Lili Xu","doi":"10.1016/j.virs.2025.01.003","DOIUrl":"10.1016/j.virs.2025.01.003","url":null,"abstract":"<div><div>Respiratory syncytial virus (RSV) is one of the most common viruses leading to lower respiratory tract infections (LRTIs) in children and elderly individuals worldwide. Although significant progress in the prevention and treatment of RSV infection was made in 2023, with two anti-RSV vaccines and one monoclonal antibody approved by the FDA, there is still a lack of postinfection therapeutic drugs in clinical practice, especially for the pediatric population. In recent years, with an increasing understanding of the pathogenic mechanisms of RSV, drugs and drug candidates, have shown great potential for clinical application. In this review, we categorize and discuss promising anti-RSV drug candidates that have been in preclinical or clinical development over the last five years.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 147-156"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Virologica SinicaPub Date : 2025-04-01DOI: 10.1016/j.virs.2025.01.004
Yousong Peng , Lei Yang , Weijuan Huang , Mi Liu , Xiao Ding , Xiangjun Du , Yuelong Shu , Taijiao Jiang , Dayan Wang
{"title":"Evaluating the performance of the PREDAC method in flu vaccine recommendations over the past decade (2013–2023)","authors":"Yousong Peng , Lei Yang , Weijuan Huang , Mi Liu , Xiao Ding , Xiangjun Du , Yuelong Shu , Taijiao Jiang , Dayan Wang","doi":"10.1016/j.virs.2025.01.004","DOIUrl":"10.1016/j.virs.2025.01.004","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 288-291"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Virologica SinicaPub Date : 2025-04-01DOI: 10.1016/j.virs.2025.02.002
Zhongzhong Tu , Tong Wang , Yu Xu , Heting Sun , Peng Peng , Siyuan Qin , Changchun Tu
{"title":"Identification and genetic analysis of new ephemeroviruses in wild boars in China","authors":"Zhongzhong Tu , Tong Wang , Yu Xu , Heting Sun , Peng Peng , Siyuan Qin , Changchun Tu","doi":"10.1016/j.virs.2025.02.002","DOIUrl":"10.1016/j.virs.2025.02.002","url":null,"abstract":"<div><div>Ephemeroviruses (EVs) are arthropod-borne rhabdoviruses and were isolated exclusively from cattle and haematophagous arthropods until two new ephemeroviruses were first identified from domestic pigs most recently. Here we report the identification of newer EVs in wild boar by meta-transcriptomic (MTT) sequencing. Further screening by specific RT-nPCR of tissue samples of 459 free-ranging wild boars collected between 2018 and 2023 from 26 provinces across China confirmed five positive wild boars in four provinces. Interestingly, two ticks especially collected from two positive wild boars were also EV positive. Finally, four complete genome sequences of wild boar ephemeroviruses (WbEVs) were obtained with two strains belonging to a new EV species, and the rest two falling into porcine ephemerovirus 2 (PoEV2) species identified from domestic pigs. Our study has further extended EV host range and demonstrated natural circulations of divergent EVs in wild boars, in which ticks may play roles. Biological implications of EV infection in wild boars should be interesting topics for future investigations.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 186-191"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Virologica SinicaPub Date : 2025-04-01DOI: 10.1016/j.virs.2025.03.001
Xiwen Zhao , Xuan Dai , Fuyi Wang , Chenyang Li , Xun Song , Yingying Han , Chaowei Zhang , Lu Wang , Zhendan He , Rongping Zhang , Liang Ye
{"title":"Artemisia annua L. leaf extracts suppress influenza virus infection by targeting the viral nucleoprotein and blocking mitochondria-mediated apoptosis","authors":"Xiwen Zhao , Xuan Dai , Fuyi Wang , Chenyang Li , Xun Song , Yingying Han , Chaowei Zhang , Lu Wang , Zhendan He , Rongping Zhang , Liang Ye","doi":"10.1016/j.virs.2025.03.001","DOIUrl":"10.1016/j.virs.2025.03.001","url":null,"abstract":"<div><div><em>Artemisia annua</em> L. is a medicinal herb with multiple therapeutic applications, whereas its anti-influenza A virus (IAV) efficiency and mechanism of action are still unclear. Here, we investigated the inhibition activity and mechanism of <em>A. annua</em> leaf methanol extracts (AALME) against IAV <em>in vitro</em> and <em>in vivo</em>. Our results revealed that AALME exhibits potent anti-IAV activity by interacting with IAV particles. Mechanistically, AALME directly targets the IAV nucleoprotein (NP) protein and abolishes the nuclear import of IAV NP. AALME profoundly suppresses IAV-induced mitochondrial apoptosis via suppressing ROS-mediated AIF-dependent pathways. More importantly, we found that AALME plays a crucial role in protecting mice from IAV infection and mitigating IAV pathogenicity. This current work provides mechanistic insight into the mechanism by which AALME controls IAV infection <em>in vitro</em> and <em>in vivo</em>, potentially contributing to the development of antiviral treatments for IAV infection.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 247-259"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Virologica SinicaPub Date : 2025-04-01DOI: 10.1016/j.virs.2025.03.003
Yixin Hu , Zehui Li , Chenlin Hao , Hao Lu , Yunfei Xing , Kexin Liu , Xiaohui Jin , Zhanyong Wei
{"title":"Identification of an immunodominant neutralizing epitope of porcine astrovirus type 5 capsid protein","authors":"Yixin Hu , Zehui Li , Chenlin Hao , Hao Lu , Yunfei Xing , Kexin Liu , Xiaohui Jin , Zhanyong Wei","doi":"10.1016/j.virs.2025.03.003","DOIUrl":"10.1016/j.virs.2025.03.003","url":null,"abstract":"","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 296-299"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Virologica SinicaPub Date : 2025-04-01DOI: 10.1016/j.virs.2025.03.009
Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen
{"title":"Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies","authors":"Jielin Tang , Sijie Chen , Yi Zhong , Yijun Deng , Dan Huang , Junjun Liu , Yi Zheng , Jiyuan Xu , Bao Xue , Fan Wang , Yuan Zhou , Hanzhong Wang , Qi Yang , Xinwen Chen","doi":"10.1016/j.virs.2025.03.009","DOIUrl":"10.1016/j.virs.2025.03.009","url":null,"abstract":"<div><div>Human bocavirus 1 (HBoV1; family: <em>Parvoviridae</em>) causes a wide spectrum of respiratory diseases in children and gastroenteritis in adults. A lack of sensitive cell lines and efficient animal models hinders research on HBoV, including the development of anti-HBoV drugs or vaccines. Although the construction of a wild-type HBoV1 infectious clone has been reported, generating HBoV1 infectious clone carrying foreign reporter genes with suitable insertion sites in its genome while retaining replicative ability remains challenging. Here, HBoV1 infectious clones harboring the 11-amino-acid HiBiT tag at five distinct insertion sites were constructed and evaluated. Only the recombinant HBoV1 carrying the HiBiT tag in the N-terminus of the NS1 protein (HBoV1-HiBiT<sub>NS1</sub>) displayed comparable characteristics to wild-type HBoV1 as determined via the analysis of viral DNA copy number, NanoLuc activity, viral protein expression, and the formation of replication intermediates. Notably, the replication kinetics of HBoV1-HiBiT<sub>NS1</sub> could be examined by monitoring NanoLuc activity, which was noted to be correlated with the viral DNA level. Additionally, we successfully applied HiBiT-tagged HBoV1 for the evaluation of antiviral drug activity and identified ivermectin (EC50 = 2.27 μM) as a potent anti-HBoV1 replication drug. Overall, our study demonstrated that the HBoV1-HiBiT<sub>NS1</sub> reporter can serve as a convenient platform for screening candidate drugs targeting HBoV1 replication and may also be useful for investigating the life cycle of the virus.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 275-283"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}