Virologica Sinica最新文献_第3页

The SARS-CoV-2 NSP4 T492I mutation promotes double-membrane vesicle formation to facilitate transmission SARS-CoV-2 NSP4 T492I 突变促进了双膜囊泡的形成，从而有利于传播。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.010

Pei Wang , Buyun Tian , Ke Xiao , Wei Ji , Zonghong Li

{"title":"The SARS-CoV-2 NSP4 T492I mutation promotes double-membrane vesicle formation to facilitate transmission","authors":"Pei Wang , Buyun Tian , Ke Xiao , Wei Ji , Zonghong Li","doi":"10.1016/j.virs.2025.03.010","DOIUrl":"10.1016/j.virs.2025.03.010","url":null,"abstract":"<div><div>The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in mutations not only in the spike protein, aiding immune evasion, but also in the NSP3/4/6 proteins, crucial for regulating double-membrane vesicle (DMV) formation. However, the functional consequences of these NSP3/4/6 mutations remain poorly understood. In this study, a systematic analysis was conducted to investigate the evolutionary patterns of NSP3/4/6 mutations and their impact on DMV formation. The findings revealed that the NSP4 T492I mutation, a prevalent mutation found in all Delta and Omicron sub-lineages, notably enhances DMV formation. Mechanistically, the NSP4 T492I mutation enhances its homodimerization, leading to an increase in the size of puncta induced by NSP3/4, and also augments endoplasmic reticulum (ER) membrane curvature, resulting in a higher DMV density per fluorescent puncta. This study underscores the significance of the NSP4 T492I mutation in modulating DMV formation, with potential implications for the transmission dynamics of SARS-CoV-2. It contributes valuable insights into how these mutations impact viral replication and pathogenesis.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 225-235"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-function insights into the dual role of African swine fever virus pB318L: A typical geranylgeranyl-diphosphate synthase and a nuclear import protein 非洲猪瘟病毒pB318L的结构-功能双重作用：一种典型的香叶二磷酸合成酶和一种核输入蛋白。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.013

Hai-Fan Zhao , Ying Wang , Xiao-Hong Liu , Xian-Hui Liu , Zhi Geng , Zeng-Qiang Gao , Li Huang , Chang-Jiang Weng , Yu-Hui Dong , Heng Zhang

{"title":"Structure-function insights into the dual role of African swine fever virus pB318L: A typical geranylgeranyl-diphosphate synthase and a nuclear import protein","authors":"Hai-Fan Zhao , Ying Wang , Xiao-Hong Liu , Xian-Hui Liu , Zhi Geng , Zeng-Qiang Gao , Li Huang , Chang-Jiang Weng , Yu-Hui Dong , Heng Zhang","doi":"10.1016/j.virs.2025.03.013","DOIUrl":"10.1016/j.virs.2025.03.013","url":null,"abstract":"<div><div>African swine fever virus (ASFV) pB318L is an important protein for viral replication that acts as a membrane-bound <em>trans</em>-geranylgeranyl-diphosphate synthase (GGPPS) catalyzing the condensation of isopentenyl diphosphate (IPP) with allylic diphosphates. Recently we solved the crystal structure pB318L lacking N-terminal transmembrane region and performed a preliminary structural analysis. In this study, structure-based mutagenesis study and geranylgeranyl pyrophosphate (GGPP) production assay further revealed the key residues for the GGPPS activity. Structural comparison showed pB318L displays a strong similarity to typical GGPPSs instead of protein prenyltransferases. The phylogenetic analysis indicated pB318L may share a common ancestor with the GGPPSs from <em>Brassicaceae</em> plants rather than from its natural host. The subcellular localization analysis showed pB318L is localized in both nucleus and cytoplasm (including the endoplasmic reticulum membrane and mitochondria outer membrane). A unique N-terminal nuclear localization signal (NLS) following the transmembrane region was discovered in pB318L and the NLS was confirmed to be required for the nuclear import. We further revealed the NLS plays an essential role in the interaction with nuclear transporter karyopherin subunit alpha 1 (KPNA1). Their interaction may suppress signal transducers and activators of transcription 1 (STAT1) translocation and subsequently competitively inhibit nuclear import of IFN-stimulated gene factor 3 (ISGF3) complex. Our biochemical, structural and cellular analyses provide novel insights to pB318L that acts as an essential GGPPS that promotes viral replication and as a nuclear import protein that may be involved in immune evasion of ASFV.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 236-246"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zika virus transmission in Aedes aegypti: A systematic study on the ability of mosquitoes to transmit the virus horizontally and vertically 寨卡病毒在埃及伊蚊中的传播：蚊子水平和垂直传播病毒能力的系统研究。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.02.001

Xugang Wang , Chaonan Qian , Chenxi Zhang , Siyun Hu , Muhammad Asad , Chengjie Yang , Bingrui Liao , Xiaotong Guo , Chen Zhang , Quanzhi Li , Xinyao Li , Qiuying Huang , Youhui Si , Bibo Zhu , Shengbo Cao , Jing Ye

{"title":"Zika virus transmission in Aedes aegypti: A systematic study on the ability of mosquitoes to transmit the virus horizontally and vertically","authors":"Xugang Wang , Chaonan Qian , Chenxi Zhang , Siyun Hu , Muhammad Asad , Chengjie Yang , Bingrui Liao , Xiaotong Guo , Chen Zhang , Quanzhi Li , Xinyao Li , Qiuying Huang , Youhui Si , Bibo Zhu , Shengbo Cao , Jing Ye","doi":"10.1016/j.virs.2025.02.001","DOIUrl":"10.1016/j.virs.2025.02.001","url":null,"abstract":"<div><div>Zika virus (ZIKV) is a mosquito-borne virus belonging to the genus <em>Orthoflavivirus</em>, and the family <em>Flaviviridae</em>. It commonly presents with febrile-like symptoms, neurological issues, and pregnancy complications in humans. Currently, there is no commercial vaccine or specific treatment available to prevent ZIKV infection. Therefore, controlling the epidemic's spread relies on preventing mosquitoes from transmitting the virus. Although various studies have explored the transmission of ZIKV between mosquitoes and vertebrate hosts, comprehensive research on potential mosquito-to-mosquito transmission of ZIKV remains limited. In this study, we conducted systematic laboratory investigations to assess the ability of ZIKV to spread among mosquitoes, and to evaluate the impact of ZIKV infection on mosquito development. Our findings revealed that ZIKV can be transmitted between <em>Aedes aegypti</em> mosquitoes both vertically and horizontally, through oviposition and contact between mosquitoes of the same or opposite sex. Additionally, we observed that ZIKV infection resulted in a reduction in the number of mosquito eggs but an increase in their size. The widespread distribution of ZIKV in infected mosquitoes and the altered levels of hormone related genes following viral infection were noted, which may contribute to viral transmission among mosquitoes and affect mosquito development. This research provides systematic experimental evidence of ZIKV transmission among mosquitoes, which is crucial for developing novel strategies to disrupt the spread of orthoflaviviruses and other mosquito-borne pathogens.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 192-205"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological and molecular characteristics of human parainfluenza virus in southern China during 2016–2020 2016-2020年华南地区人副流感病毒流行病学及分子特征分析

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.004

Yizhe Li , Minjie Liu , Jingyao Liang , Hengming Ye , Mingcui Lyu , Delin Chen , Linyue Liang , Shuqing Zhang , Kexin Zhang , Shu An , Wenle Zhou , Jueheng Wu , Xun Zhu , Zhenjian He

{"title":"Epidemiological and molecular characteristics of human parainfluenza virus in southern China during 2016–2020","authors":"Yizhe Li , Minjie Liu , Jingyao Liang , Hengming Ye , Mingcui Lyu , Delin Chen , Linyue Liang , Shuqing Zhang , Kexin Zhang , Shu An , Wenle Zhou , Jueheng Wu , Xun Zhu , Zhenjian He","doi":"10.1016/j.virs.2025.03.004","DOIUrl":"10.1016/j.virs.2025.03.004","url":null,"abstract":"<div><div>Human parainfluenza viruses (HPIV) are common viral pathogens in acute respiratory infection (ARI). We aimed to describe the epidemiological and molecular characteristics of HPIV from ARI patients. This cross-sectional study was conducted using respiratory samples from 9,696 ARI patients between 2016 and 2020 in southern China. All samples were analyzed by quantitative real-time polymerase chain reaction to determine the presence of HPIV and other common respiratory viruses. Descriptive statistics were performed to determine the temporal and population distribution of HPIV. The full-length hemagglutinin-neuraminidase (<em>HN</em>) gene of HPIV3-positive samples was sequenced for phylogenetic analysis. A total of 577 (6.0%) patients tested positive for HPIV, with HPIV3 being the predominant serotype, accounting for 46.8% of cases. Notably, 66.0% of these HPIV-positive cases were children aged 0–2 years. The prevalence of HPIV infections showed a decreased trend and altered peak during 2016–2020. Cough, fever, sputum production, and rhinorrhea were common respiratory symptoms in HPIV-positive patients. The majority of cases had pneumonia (63.4%). Human rhinovirus (HRV) and human coronavirus (HCoV) were the most common coinfection viruses in HPIV-positive cases, with proportions of 20.1% and 14.4%, respectively. Phylogenetic analysis revealed that the predominant lineage of HPIV3 was C3f (86.0%), followed by lineage C3a (8.0%), C3d (4.0%), and C3b (2.0%). These findings help to better understand the epidemiology of HPIV, and improve public health strategies to prevent and control HPIV infections in southern China.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 157-165"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenitc analysis and immunogenicity comparison of porcine genotype G9 rotavirus in China from 2020–2023 2020-2023年中国猪基因型G9轮状病毒系统发育分析及免疫原性比较

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.02.003

Yudi Pan, Zixin Li, Qian Miao, Hongyan Shi, Longjun Guo, Li Feng, Jin Tian

{"title":"Phylogenitc analysis and immunogenicity comparison of porcine genotype G9 rotavirus in China from 2020–2023","authors":"Yudi Pan, Zixin Li, Qian Miao, Hongyan Shi, Longjun Guo, Li Feng, Jin Tian","doi":"10.1016/j.virs.2025.02.003","DOIUrl":"10.1016/j.virs.2025.02.003","url":null,"abstract":"<div><div>As an emerging genotype, the G9 genotype rotaviruses (RVs) are widespread among humans and pigs, and have been reported in many countries and regions in the recent years. Moreover, porcine G9 strains could cross the interspecies barrier to infect human. To investigate the epidemic trends of porcine G9 strains as well as the cross-immunoreactivity among different isolates, an epidemiological investigation about porcine G9 genotype RVs (PoRVs) was performed during the period 2020–2023 in multiple provinces of China. A total of nine representative strains were identified. The phylogenetic analysis based on viral <em>VP7</em> gene showed that these strains mainly clustered with lineages III and VI, which revealed the predominant G9 PoRVs in China. Moreover, a new lineage, lineage VII, was identified, and strains of this lineage were found to be circulating in Guangdong and Taiwan. Except lineages I and IV, some isolates from other lineages could co-circulate in pigs and humans. Three G9 strains, namely 923H, 923E, and 923X, which belonged to the largest sub-lineage III, were isolated. Then, the significant cross-reactivity was observed among strains of the same or different lineages. This study is the first to systematically investigate the genetic and immunogenetic characteristics of porcine G9 genotype rotavirus in China, as well as the potential cross-species transmission between pigs and humans, providing a valuable direction for the effective prevention of porcine rotavirus.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 176-185"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo quantitative proteomic analysis of porcine alveolar macrophages in PRRSV-infected pigs prrsv感染猪肺泡巨噬细胞的体内定量蛋白质组学分析。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.002

Ying Wei , Chuangchao Zou , Siying Zeng , Ouyang Peng , Guangli Hu , Yihui Huang , Qiuping Xu , Fangyu Hu , Yongchang Cao , Hao Zhang

{"title":"In vivo quantitative proteomic analysis of porcine alveolar macrophages in PRRSV-infected pigs","authors":"Ying Wei , Chuangchao Zou , Siying Zeng , Ouyang Peng , Guangli Hu , Yihui Huang , Qiuping Xu , Fangyu Hu , Yongchang Cao , Hao Zhang","doi":"10.1016/j.virs.2025.03.002","DOIUrl":"10.1016/j.virs.2025.03.002","url":null,"abstract":"<div><div>Porcine reproductive and respiratory syndrome (PRRS), a highly infectious immunosuppressive disease caused by porcine reproductive and respiratory syndrome virus (PRRSV), has led to significant economic losses in the global swine industry. The complexity of preventing and controlling PRRS, compounded by the limited efficacy of current vaccines, underscores the urgent need to identify antiviral targets and develop effective therapeutics against PRRSV. From the perspective of virus-host interactions, the discovery of target molecules associated with PRRSV resistance offers a promising strategy for future disease management. In this study, we conduct a comprehensive proteomic analysis using data-independent acquisition (DIA) mode to investigate the host response throughout the acute phase of PRRSV infection. This approach provides critical insights into the regulation of host antiviral and immune pathways during acute infection, advancing our theoretical understanding of PRRSV-host interactions and host gene dynamics during this critical phase. Notably, we identified SCARB2, a major lysosomal membrane protein associated with cholesterol metabolism, as a potential regulator of PRRSV replication. These findings offer novel perspectives for the prevention and control of PRRSV, contributing to the development of targeted antiviral strategies.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 206-216"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virus profiling of bronchoalveolar lavage fluid in hospitalized non-COVID-19 adult patients with pulmonary infection from November 2020 to November 2021 2020年11月至2021年11月住院非covid -19成人肺部感染患者支气管肺泡灌洗液病毒谱分析

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.03.005

Liangyu Li , Haiyue Zhang , Pei Xiong , Chan Liu , Lu Wan , Mengling Liu , Jieyu Mao , Ruiyun Li , Min Shang , Hailing Liu , Yuchuan Luo , Jing Yin , Xiaojun Wu , Jianjun Chen

{"title":"Virus profiling of bronchoalveolar lavage fluid in hospitalized non-COVID-19 adult patients with pulmonary infection from November 2020 to November 2021","authors":"Liangyu Li , Haiyue Zhang , Pei Xiong , Chan Liu , Lu Wan , Mengling Liu , Jieyu Mao , Ruiyun Li , Min Shang , Hailing Liu , Yuchuan Luo , Jing Yin , Xiaojun Wu , Jianjun Chen","doi":"10.1016/j.virs.2025.03.005","DOIUrl":"10.1016/j.virs.2025.03.005","url":null,"abstract":"<div><div>Identifying the cause of respiratory tract infections is important for reducing the burden of diagnosis and treatment. To assess viral etiologies of hospitalized patients with pulmonary infection, bronchoalveolar lavage fluid (BALF) specimens were collected from non-COVID-19 adult patients (n = 333, including patients with lower respiratory tract infection, tuberculosis, lung cancer, and pulmonary nodules) between November 2020 and November 2021. Multiple common respiratory pathogens were detected using multiplex reverse-transcription polymerase chain reaction. The result showed that at least one virus was identified in 35.44% (118/333) of the cases. Among these, influenza virus was the most commonly identified, followed by the parainfluenza virus, coronavirus, human rhinoviruses, and human respiratory syncytial viruses. The tuberculosis group demonstrated the highest viral detection rate, yet paradoxically exhibited the lowest co-infection rate. In contrast, the highest co-infection frequency was observed in the pulmonary nodules group. Patients with viral infections exhibited more severe clinical symptoms compared to those without detected viral infections. However, this observation was only noted in the lower respiratory tract infection group among the different disease groups. Notably, among patients infected with a specific virus, there were no significant differences in viral load between single and co-infections. Our study identified the major causative agents in hospitalized adult patients with pulmonary infection, offering insights for precise disease diagnosis and the prevention of unnecessary use of antimicrobial drugs.</div></div>","PeriodicalId":23654,"journal":{"name":"Virologica Sinica","volume":"40 2","pages":"Pages 166-175"},"PeriodicalIF":5.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current antiviral therapies and promising drug candidates against respiratory syncytial virus infection 目前抗呼吸道合胞病毒感染的抗病毒疗法和有希望的候选药物。

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.01.003

Ziheng Feng , Zhengde Xie , Lili Xu

引用次数: 0

Evaluating the performance of the PREDAC method in flu vaccine recommendations over the past decade (2013–2023) 评估过去十年（2013-2023）PREDAC方法在流感疫苗推荐中的表现

IF 5.5 3区医学

Virologica Sinica Pub Date : 2025-04-01 DOI: 10.1016/j.virs.2025.01.004

Yousong Peng , Lei Yang , Weijuan Huang , Mi Liu , Xiao Ding , Xiangjun Du , Yuelong Shu , Taijiao Jiang , Dayan Wang

引用次数: 0

Sphingomyelin phosphodiesterase 3 as a novel host factor inhibiting hepatitis B virus transcription 鞘磷脂二酯酶3作为抑制乙型肝炎病毒转录的新宿主因子。