Veterinary parasitology, regional studies and reports最新文献

{"title":"A profiling of faecal parasites in horses in Mauritius and resistance to Fenbendazole at one horse-riding stable","authors":"N. Nayeelah Golamgouse","doi":"10.1016/j.vprsr.2025.101261","DOIUrl":"10.1016/j.vprsr.2025.101261","url":null,"abstract":"<div><div>Mauritius hosts a population of approximately 900 equids, but no investigations have delved into issues such as the prevalence of equine helminths, anthelmintic usage, and resistance emergence.</div><div>This study aimed to devise a qualitative and quantitative profile of common helminthic intestinal parasites in equines at eight out of twenty-eight stables (272 horses, 29 % of local population) and to determine whether helminthic intestinal parasites in equines at one stable have developed resistance to Fenbendazole by quantitatively assessing pre-treatment faecal egg counts, post-treatment faecal egg counts and percentage faecal egg count reduction (%FECR).</div><div>Targeted parasites included <em>Parascaris equorum</em>, <em>strongyles</em> (<em>Cyathostomum</em> spp. and <em>Strongylus</em> spp.), <em>Fasciola hepatica</em>, <em>Dictyocaulus arnfieldi</em>, <em>Trichostrongylus axei</em>, and <em>Strongyloides westeri</em>, identified based on their morphological characteristics and relative size.</div><div>Results indicated a prevalence for <em>P. equorum</em> in 31 % and strongyles in 22 % of horses sampled. No other helminth eggs were detected in any of the samples studied. Quantitative analysis, employing the McMaster Technique for faecal egg count (FEC), revealed that 99 % and 98 % of horses sampled exhibited FEC &lt; 200 eggs per gram (epg) for <em>P. equorum</em> and strongyles respectively, categorizing them as low shedders by AAEP standards. Two percent of horses were classified as moderate strongyle egg shedders (between 200 and 500 epg), and 0 % were high strongyle egg shedders (more than 500 epg). For <em>P. equorum</em>, 0.4 % of horses were categorized as moderate egg shedders and 0.4 % were high egg shedders. No significant correlations were discerned between FEC and stable parameters such as location and size.</div><div>At one stable, fenbendazole (FBZ) was administered orally at a dosage of 5 mg/kg via stomach tube to 24 equids. Pre-treatment FEC values were compared to post-treatment FECs taken 14 days after anthelmintic administration, with the %FECR computed. Mean %FECR and adjusted mean %FECR for <em>P. equorum</em> were 91 % and 92 % respectively, which suggested drug efficacy. For strongyle, mean %FECR and adjusted mean %FECR were 59 % and 98 % respectively, which also seemingly supported the hypothesis of lack of resistance. Bayesian analysis however, revealed only a 63 % probability (i.e. &lt;95 %) that true reduction exceeds 90 %. The findings suggested the presence or imminent presence of resistance to fenbendazole at this stable and emphasized the imperative for judicious anthelmintic administration coupled with a surveillance-oriented approach to mitigate resistance emergence in other stables. Furthermore, the pressing need to adopt alternative integrated control measures is emphasized, reflecting the urgency in addressing this burgeoning challenge in equine health management.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"61 ","pages":"Article 101261"},"PeriodicalIF":1.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoonotic gastrointestinal parasites of shelter dogs in Israel","authors":"H. Salant ,&nbsp;D. Yasur-Landau ,&nbsp;S.-L. Siboni ,&nbsp;Y. Nachum Biala ,&nbsp;G. Baneth","doi":"10.1016/j.vprsr.2025.101258","DOIUrl":"10.1016/j.vprsr.2025.101258","url":null,"abstract":"<div><div>Dogs are potentially infected with a diverse spectrum of endoparasites, some which may affect their health or be of zoonotic concern. In Israel, no information exists on the prevalence and spatial distribution of endoparasitic infection among dogs. An investigation of the prevalence of endoparasite infection in Israel shelter dogs was performed by fecal flotation and molecular methods, with particular interest in parasites of zoonotic concern. Polymerase-chain reaction (PCR) was performed targeting the small subunit ribosomal RNA <em>(rrnS)</em> of <em>Taenia</em> spp.,-<em>cox1</em> mitochondrial DNA of <em>Strongyloides</em> spp., and <em>18S rRNA</em> of <em>Cryptosporidium</em> spp. The study included fecal samples of 393 dogs from six municipal shelters in different areas of Israel. Overall, 92 of 393 (23.4 %) dogs were infected with at least one endoparasite. According to fecal microscopy, 7.4 % were infected with <em>Cystoisospora</em> spp.; 7.1 % with <em>Giardia intestinalis</em>; 1.0 % with <em>Toxocara canis</em>; 3.1 % with <em>Toxascaris leonina</em>; 2.5 % with <em>Sarcocystis</em> spp.; 2.3 % shed taeniid eggs; 0.3 % <em>Spirocerca lupi</em> eggs; 0.3 % hookworm spp. eggs and 0.3 % shed <em>Hammondia heydorni</em> oocysts. PCR for <em>Taenia</em> spp. detected 1.8 % dogs with <em>Taenia hydatigena</em>, and 0.51 % dogs with <em>Taenia</em> spp. eggs.</div><div>Several epidemiologically significant points were identified; <em>Ta. leonina</em> was more prevalent in dogs from the north of Israel, while <em>Sarcocystis</em> spp. and <em>Taenia</em> eggs were more prevalent in the south. In addition, infection with <em>Ta. leonina</em> was found to be significantly increased in summer compared to other seasons. This is the first large scale study of endoparasites in dogs from Israel, with results showing presence of potentially zoonotic parasites, <em>T. canis, Giardia</em> spp. and hookworms. These results should serve to increase awareness of veterinarians, human infectious disease specialists, and dog shelter personnel of relevant parasites to plan better prevention, diagnosis and treatment schemes in dog shelters and to look for suggestive signs of endoparasite-related disease in a One Health framework.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"61 ","pages":"Article 101258"},"PeriodicalIF":1.4,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A history of Trypanosoma cruzi screening in domestic dogs throughout the Americas – A systematic review","authors":"Wendy Pace","doi":"10.1016/j.vprsr.2025.101260","DOIUrl":"10.1016/j.vprsr.2025.101260","url":null,"abstract":"<div><div>Chagas disease is a known killer of humans and other species ranging from South America north into the United States with an estimated 6–7 million human infections globally. Interest in canine Chagas disease has been high since its discovery in 1909 due to the intertwined relationships between humans and domestic dogs but no agency exists that records or tracks canine infections. A systematic review was conducted with the objectives of compiling and examining all available data originally in English, Spanish or Portuguese containing raw numbers referencing a screening effort in naturally infected or exposed domestic dogs from the Americas since its discovery. A total of 170 publications were identified and included for review. Study areas, sample groups, infection rates, methods of diagnosis, and demographics were discussed for comparison and historical perspective. The passage of time has seen numerous advances in diagnostic methods and the promise of effective treatment options, for humans and dogs, but there is still a long way to go in establishing standard diagnostic methods and providing clinically accessible treatment options.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"60 ","pages":"Article 101260"},"PeriodicalIF":1.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tale of a snail: Fatal meningoencephalitis caused by Angiostrongylus cantonensis in a spider monkey (Ateles chamek) and detection among snails, São Paulo, Brazil","authors":"Natália C.C. de A. Fernandes ,&nbsp;Eduardo Ferreira-Machado ,&nbsp;Jamile Macedo Garcia ,&nbsp;Pedro Enrique Navas-Suarez ,&nbsp;Sarah Raquel Jesus Santos Simões ,&nbsp;Ana Carolina Souza Ramos de Carvalho ,&nbsp;Patricia Sayuri Silvestre Matsumoto ,&nbsp;André Luiz Mota da Costa ,&nbsp;Mariana Castilho Martins ,&nbsp;Rodrigo Hidalgo Friciello Teixeira ,&nbsp;Cássia Regina Ramos Gonzaga ,&nbsp;Marina Alvarado de Medeiros ,&nbsp;Shamira de Fátima Sallum Leandro ,&nbsp;Flora Nogueira Matos ,&nbsp;Leyva Cecilia Vieira de Melo ,&nbsp;Amanda de Oliveira Baccin ,&nbsp;Dan Jessé Gonçalves da Mota ,&nbsp;Ricardo Gava ,&nbsp;Liliane Ré de Luca ,&nbsp;Thais Eleonora Madeira Buti ,&nbsp;Juliana Mariotti Guerra","doi":"10.1016/j.vprsr.2025.101262","DOIUrl":"10.1016/j.vprsr.2025.101262","url":null,"abstract":"<div><div><em>Angiostrongylus cantonensis</em> causes eosinophilic meningitis in humans and animals. We report a fatal case of <em>A. cantonensis</em> in a spider monkey, with descriptive microscopic findings, molecular diagnosis and a subsequent environmental investigation at a zoo in Sorocaba municipality, Brazil. This case highlights the importance of One Health surveillance for parasitic zoonosis.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"60 ","pages":"Article 101262"},"PeriodicalIF":1.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodiversity, prevalence, and risk factors associated with tick infestation of Indigenous Nigerian breed of hunting dogs in Kwara State, Nigeria","authors":"Shola David Ola-Fadunsin ,&nbsp;Isau Aremu Ganiyu ,&nbsp;Hauwa Motunrayo Ambali ,&nbsp;Fufa Ido Gimba ,&nbsp;Donea Abdulrazak Abdullah ,&nbsp;Khalida H. Younis","doi":"10.1016/j.vprsr.2025.101256","DOIUrl":"10.1016/j.vprsr.2025.101256","url":null,"abstract":"<div><div>Ticks are hematophagous arthropods that cause paralysis, toxicosis, and more importantly, they harbor and transmit different pathogens affecting humans and animals. This study was aimed to investigate the species composition, richness, diversity indices, prevalence, and risk factors associated with tick infestation of indigenous Nigerian breed of hunting dogs in Kwara State, Nigeria. The study was conducted on 240 hunting dogs from six local government areas in Kwara State. The dogs were subjected to integumentary examinations and the collected ticks were collected in 70% ethanol and transported to the Laboratory where they were morphologically identified using standard entomological keys. Of the 240 hunting dogs sampled, 157 were infested with ticks representing 65.4% with a 95% CI of 59.2–71.2. A total of 1206 ticks were collected belonging to <em>Amblyomma variegatum</em>, <em>Haemaphysalis leachi</em>, <em>Hyalomma rufipes</em>, <em>Rhipicephalus decoloratus</em>, <em>Rhipicephalus microplus</em>, and <em>Rhipicephalus linnaei</em> (Audouin, 1826), with <em>Rhipicephalus linnaei</em> being the most prevalent. The biodiversity and species richness indices, the prevalence of the infestation patterns, cumulative counts and mean load, species of ticks based on their developmental stages and sexes, as well as the degree of tick infestation were determined. Age was the only risk index significantly associated with the infestation of ticks among hunting dogs in the study. These findings underscore the importance of implementing tick control programs for hunting dogs, which may serve as reservoirs of ticks and tick-borne pathogens for domestic and pet dogs and humans.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"60 ","pages":"Article 101256"},"PeriodicalIF":1.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of bald eagle (Haliaeetus leucocephalus) from Alaska, USA as a natural definitive host for Sarcocystis infections of bovid and cervid intermediate hosts","authors":"Aditya Gupta ,&nbsp;Kimberlee Beckmen ,&nbsp;Benjamin M. Rosenthal ,&nbsp;Jitender P. Dubey","doi":"10.1016/j.vprsr.2025.101259","DOIUrl":"10.1016/j.vprsr.2025.101259","url":null,"abstract":"<div><div><em>Sarcocystis</em> sporocysts were found in intestinal scrapings of two Bald eagles (<em>Haliaeetus leucocephalus</em>) from Alaska, USA. The sporocysts/oocysts were characterized molecularly using <em>Sarcocystis</em>-specific primers targeting the <em>18S</em> rRNA, <em>28S</em> rRNA, <em>cox1,</em> and a region spanning <em>18S</em> rRNA, <em>ITS1</em>, and <em>5.8S</em> rRNA genes. The PCR amplification confirmed the presence of three <em>Sarcocystis</em> species in the bald eagles. They are: either of <em>Sarcocystis bovini</em>-like or <em>Sarcocystis bovifelis</em>-like parasites of European cattle (<em>Bos taurus</em>), <em>Sarcocystis</em> sp. of elk (<em>Cervus canadensis</em>), and either of <em>Sarcocystis tarandi-</em>like of deer (<em>Rangifer tarandus</em>) or <em>Sarcocystis elongata</em>-like parasites of red deer (<em>Cervus elaphus</em>) with identities of &gt;98 %. This is the first report of <em>H. leucocephalus</em> as a definitive host for cervid and bovid infections. It is also the first report of <em>S. bovini</em>-like <em>/ S. bovifelis</em>-like and <em>S. tarandi</em>-like / <em>S. elongata</em>-like parasites in the USA. Epidemiologic significance of findings is discussed<strong>.</strong></div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"61 ","pages":"Article 101259"},"PeriodicalIF":1.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case report: Clinical and surgical management of Dioctophyme renale in a dog","authors":"Charles Hebert Guedes ,&nbsp;Barbara Sarrapio Freire ,&nbsp;Paula Rodrigues Natividade Mansur ,&nbsp;Karinna de Oliveira Cerutti ,&nbsp;Mayara Forastieri de Souza Chagas ,&nbsp;Rhayra Silva Reis ,&nbsp;Felipe Ribeiro Alves","doi":"10.1016/j.vprsr.2025.101257","DOIUrl":"10.1016/j.vprsr.2025.101257","url":null,"abstract":"<div><div>This report presents a case of parasitism by the renal worm <em>Dioctophyme renale,</em> diagnosed in the right kidney of a domestic dog. An adult female dog, mixed breed, was initially treated for a snakebite. After ultrasound, a significant number of <em>Dioctophyme renale</em> parasites were observed in the right kidney (R.K). The urine test revealed a significant number of <em>Dioctophyme renale</em> eggs without any other abnormalities. A nephrectomy was successfully performed, and the dog showed an excellent recovery after surgery. In most cases, parasitism by <em>D. renale</em> in domestic dogs is discovered by chance or only during necropsy. The objective of this study is to report the first case of parasitism by <em>D. renale</em> in the Três Corações region, in which the diagnosis and treatment were performed promptly, allowing the dog to recover completely.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"60 ","pages":"Article 101257"},"PeriodicalIF":1.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thelazia species (eyeworms) infection in cattle: Prevalence, species diversity, seasonal dynamics and its ocular effects in South Gondar zone, Northwest Ethiopia","authors":"Tewodros Alemneh ,&nbsp;Shimelis Dagnachew","doi":"10.1016/j.vprsr.2025.101254","DOIUrl":"10.1016/j.vprsr.2025.101254","url":null,"abstract":"<div><div>Thelaziasis is a neglected vector-borne disease widely distributed throughout the world and is responsible for severe economic losses in cattle. The disease is caused by parasitic nematodes of the genus <em>Thelazia</em> (Spirurida, Thelaziidae). So far, limited attention has been given to ungulate-associated <em>Thelazia</em> species though it is a highly prevalent disease that raises concerns for animal welfare and potential economic impacts. A cross sectional study was carried out between August 2023 to July 2024 in the South Gondar Zone of Northwest Ethiopia, aiming to estimate the prevalence, investigate the seasonal dynamics, identify the species involved, demonstrate the ocular effects, and to assess the potential risk factors associated with the occurrence of thelaziasis in cattle. A multistage sampling technique was employed to select study animals. Data analysis was performed with logistic regression using SPSS software version 27.0. A total of 690 cattle were examined, of which 165 tested positive for <em>Thelazia,</em> resulting in an overall prevalence of 23.9 % (95 % CI: 20.7–27.2). <em>Thelazia rhodesi</em> and <em>Thelazia gulosa</em> were the identified species infecting cattle. Sex, body condition score and district of cattle were not associated with the occurrence of thelaziasis (<em>p</em> &gt; 0.05). However, age, breed, management and season had influenced the occurrence of parasitosis (<em>p</em> &lt; 0.05). Adult cattle (30.6 %), local breed (26.9 %), and extensive management system (29.8 %) categories have demonstrated higher infection rates than young (15.3 %), crossbreed (16.5 %), and semi-intensive management system (10.5 %). The occurrence of infection showed apparent seasonality (<em>p</em> &lt; 0.001) with the maximum prevalence appeared in autumn (39.7 %). Ocular lesions such as lacrimation, conjunctivitis and ulceration were detected in 142 (20.6 %) of examined cattle, and the occurrence of ocular lesions exhibited significant association with the prevalence of parasitosis (<em>p</em> &lt; 0.001). The occurrence of <em>Thelazia</em> species with high prevalence in the study area alarms the significance of incorporating this parasite in the differential diagnosis of eye diseases in cattle, especially when adults affected in areas with free grazing management system.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"61 ","pages":"Article 101254"},"PeriodicalIF":1.4,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Capillaria spp. and other endoparasites of hunting dogs from southern Italy","authors":"Alessia Ricci ,&nbsp;Marcos Antônio Bezerra-Santos ,&nbsp;Renata Fagundes-Moreira ,&nbsp;Mariaelisa Carbonara ,&nbsp;Riccardo Paolo Lia ,&nbsp;Jairo Alfonso Mendoza-Roldan ,&nbsp;Domenico Otranto","doi":"10.1016/j.vprsr.2025.101255","DOIUrl":"10.1016/j.vprsr.2025.101255","url":null,"abstract":"<div><div>Gastrointestinal and cardiopulmonary nematodes are frequently detected in dogs worldwide, posing a potential zoonotic risk. Some parasites, such as <em>Capillaria aerophila</em> and <em>Capillaria boehmi</em>, are often less considered being misdiagnosed with other trichurids. Here, we evaluated the occurrence of <em>Capillaria</em> spp. as well as of other endoparasites in hunting dogs from Basilicata region, Southern Italy. Individual faecal samples were collected from hunting dogs (<em>n</em> = 443) and examined by Mini-FLOTAC® flotation method. Additionally, 49 of the above samples were evaluated by the Baermann-Wetzel technique, and 90 sera samples were subjected to the Angio Detect™ test (IDEXX Laboratories Inc., Westbrook, Maine, USA). Out of 443 faecal samples, 251 (i.e., 56.6 %) scored positive for at least one parasite species, with hookworms being the most prevalent nematodes (i.e., 39.3 %), followed by <em>C. aerophila</em> (i.e., 20.5 %), <em>Trichuris vulpis</em> (i.e., 12.6 %) and <em>Toxocara canis</em> (i.e., 11.3 %). At the Baermann-Wetzel technique, 8.2 % (<em>n</em> = 4/49) of the faecal samples scored positive for <em>Angiostrongylus vasorum</em>, 4.1 % for Ancylostomatidae (<em>n</em> = 2/49) and 2.0 % for <em>Strongyloides</em> spp. (<em>n</em> = 1/49). A single serum sample scored positive for <em>A. vasorum</em> at the Angio Detect™ test. The results indicate that hunting dogs may harbour a high diversity of endoparasites, including those of zoonotic concern. Moreover, it is hypothesized that the occurrence of <em>C. aerophila</em> and <em>C. boehmi</em> may compromise the overall hunting performance due to the respiratory distress and impairment of scenting abilities. Therefore, control measures should be implemented to protect both dogs as well as humans living in close contact with them.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"60 ","pages":"Article 101255"},"PeriodicalIF":1.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of highly pathogenic Theileria luwenshuni in Sri Lanka: Are Jaffna sheep resistant to theileriosis?","authors":"T.M. Chathuri Sandamali ,&nbsp;Parththuran Gajavathany ,&nbsp;Meenayokini Rajeshwaran ,&nbsp;Rupika S. Rajakaruna ,&nbsp;Abdul Jabbar ,&nbsp;Piyumali K. Perera","doi":"10.1016/j.vprsr.2025.101250","DOIUrl":"10.1016/j.vprsr.2025.101250","url":null,"abstract":"<div><div>Theileriosis is a tick-borne piroplasmid infection that affects small ruminant production, resulting in significant economic losses. This study investigated theileriosis and its potential vectors in the Jaffna sheep breed in Sri Lanka. Blood (<em>n</em> = 100) and ticks (<em>n</em> = 57) were randomly collected from three farms. Management practices and other demographic data were collected using a questionnaire. Giemsa-stained blood smears facilitated microscopic analysis of piroplasms. The prevalence of theileriosis was compared between farms, age, and gender categories using a Chi-square test or a Fisher's exact test. The molecular identification was achieved using a nested PCR targeting the <em>18S rRNA</em> gene of <em>Theileria</em> spp. Subsequently, PCR amplicons were subjected to bidirectional sequencing and phylogenetic analysis. Microscopic examination and nested PCR revealed 38.0 % and 90.9 % prevalence of <em>Theileria</em> species, respectively, with a significant difference (<em>p</em> = 0.0015). The phylogenetic analysis showed that the <em>Theileria</em> spp. identified is the highly virulent <em>Theileria luwenshuni</em>. Collected tick species were: <em>Haemaphysalis bipsinosa, Haemaphysalis intermedia, Rhipicephalus haemaphysaloides, Rhipicephalus linneai</em> and <em>Hyalomma marginatum isaaci</em>; the most prevalent being <em>H. bispinosa</em> (68.4 %). This is the first report and molecular characterisation of <em>T. luwenshuni</em> in Sri Lanka<em>,</em> a highly pathogenic species recorded in small ruminants in tropical and subtropical countries but does not cause clinical signs in the Jaffna sheep breed. Findings of the current study suggest that Jaffna sheep tolerate the infection by <em>T. luwenshuni</em>. However, them being carriers poses a major risk to immunocompromised farm animals, especially the exotic sheep and goat breeds in the country.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"60 ","pages":"Article 101250"},"PeriodicalIF":1.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}