Identification and DNA barcoding of Tabanid flies in the pasture area of sheep at Mannavanur, Palani Hills, Tamil Nadu, India

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-08-08 DOI:10.1016/j.vprsr.2025.101326

G. Nagarajan , K. Kanagarajadurai , K. Pachaiyappan , Ghanshyam Yadav

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Abstract

The present study was carried out to identify the tabanid flies creating annoyance to sheep in the grazing area of SRRC, Mannavanur by means of morphological keys and DNA barcoding targeting mitochondrial cytochrome c oxidase subunit I (COI) gene. Two different kinds of tabanid flies were caught from the pasture area by graziers during the months of May & June 2021. With the help of Dept. of Agricultural Entomology, Centre for Plant Protection studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India and Zoological Survey of India, Kolkata, West Bengal, India, it was identified that Haematopota nathani (Cleg fly) and Tabanus subcinerascens (Horse fly) were the two dipteran tabanid flies causing annoyance and aching bite in sheep and shepherds. The total genomic DNA isolated from the flies were subjected to COI gene based PCR assay using the universal primer set and the resultant PCR amplified DNA fragments were cloned into E.coli based vector. The confirmed recombinant plasmids were subjected for gene sequencing protocol through outsourcing. Upon the BLAST analysis, the nucleotide sequences obtained from the flies identified by TNAU and ZSI, were having the highest identity with COI gene of Haematopota sp and Tabanus sp., respectively. The obtained nucleotide sequences were analysed using the standard bioinformatics tools. Small number of fly specimens and the analysis based on the partial nucleotide sequences of COI gene are the limitations. It was concluded that the documentation of H. nathani and T. subcinerascens as well as barcoding of both tabanid flies from Palani hills, Tamil Nadu, India, based on mitochondrial COI DNA marker had been carried out for the first time.

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印度泰米尔纳德邦帕拉尼山Mannavanur牧羊区Tabanid蝇类鉴定及DNA条形码分析

本研究采用形态学关键字和线粒体细胞色素c氧化酶亚基I （COI）基因DNA条形码技术，对马纳瓦努省SRRC牧区骚扰绵羊的虻进行了鉴定。在5月份，放牧人在牧场捕获了两种不同的虻。2021年6月。在印度泰米尔纳德邦哥印拜陀泰米尔纳德邦农业大学植物保护研究中心农业昆虫学系和印度西孟加拉邦加尔各答印度动物调查中心的帮助下，确定了Haematopota nathani （Cleg蝇）和Tabanus subcinerascens （Tabanus subcinerascens）是两种双翅目虻，引起羊和牧羊人的痛苦和叮咬。用通用引物对分离的蝇总基因组DNA进行COI基因PCR扩增，并将扩增的DNA片段克隆到大肠杆菌载体中。确定的重组质粒通过外包进行基因测序。经BLAST分析，经TNAU和ZSI鉴定得到的核苷酸序列分别与Haematopota sp和Tabanus sp.的COI基因具有最高的一致性。使用标准生物信息学工具分析获得的核苷酸序列。蝇类标本数量少，且基于COI基因部分核苷酸序列的分析是局限性。本研究首次完成了来自印度泰米尔纳德邦帕拉尼山（Palani hills）的H. nathani和T. subcinerascens虻的文献记录和基于线粒体COI DNA标记的条形码。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).