Braxton Sizemore , An-Chi Cheng , Heather D.S. Walden , Kuttichantran Subramaniam , Samantha M. Wisely , Juan M. Campos Krauer
{"title":"美国佛罗里达州一只圈养黑斑羚(Aepyceros melampus)感染大片形虫","authors":"Braxton Sizemore , An-Chi Cheng , Heather D.S. Walden , Kuttichantran Subramaniam , Samantha M. Wisely , Juan M. Campos Krauer","doi":"10.1016/j.vprsr.2025.101311","DOIUrl":null,"url":null,"abstract":"<div><div>Parasitic diseases are associated with diverse clinical signs, and veterinary professionals must be familiar with the presentations of infections in a variety of hosts. As human activity introduces new and exotic host species, expanding our understanding of pathogen effects in new species becomes integral to effective surveillance, diagnosis, and treatment. This article presents the expansion of the known host range of the American liver fluke, <em>Fascioloides magna</em>, with the infection of a farmed impala (<em>Aepyceros melampus</em>) in Marion County, Florida, USA. The gross and histopathological lesions observed are consistent with previous reports of dead-end host infections, particularly black striping across the peritoneum and abdominal viscera, a prominent pseudocyst on the visceral face of the liver, and hepatic fibrosis and biliary hyperplasia associated with tissue taken from that pseudocyst. While no whole or partial fluke was obtained from a field necropsy of the impala, amplification via cPCR and Sanger sequencing of the ITS2 region confirmed the presence of <em>Fascioloides magna</em> DNA in a frozen liver sample. This diagnostic approach was selected following the determination of an absence of fluke eggs in feces, as is characteristic of dead-end and aberrant host infections. While impala likely play little role in determining population-scale epidemiological and ecological dynamics of fluke infections, given their dead-end host status, this report serves as an important reference for managers and veterinary professionals hoping to preserve the health and welfare of exotic bovids that interface with native American cervids, the definitive hosts of <em>Fascioloides magna</em>.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101311"},"PeriodicalIF":1.4000,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fascioloides magna infection in a captive impala (Aepyceros melampus) in Florida, USA\",\"authors\":\"Braxton Sizemore , An-Chi Cheng , Heather D.S. Walden , Kuttichantran Subramaniam , Samantha M. Wisely , Juan M. Campos Krauer\",\"doi\":\"10.1016/j.vprsr.2025.101311\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Parasitic diseases are associated with diverse clinical signs, and veterinary professionals must be familiar with the presentations of infections in a variety of hosts. As human activity introduces new and exotic host species, expanding our understanding of pathogen effects in new species becomes integral to effective surveillance, diagnosis, and treatment. This article presents the expansion of the known host range of the American liver fluke, <em>Fascioloides magna</em>, with the infection of a farmed impala (<em>Aepyceros melampus</em>) in Marion County, Florida, USA. The gross and histopathological lesions observed are consistent with previous reports of dead-end host infections, particularly black striping across the peritoneum and abdominal viscera, a prominent pseudocyst on the visceral face of the liver, and hepatic fibrosis and biliary hyperplasia associated with tissue taken from that pseudocyst. While no whole or partial fluke was obtained from a field necropsy of the impala, amplification via cPCR and Sanger sequencing of the ITS2 region confirmed the presence of <em>Fascioloides magna</em> DNA in a frozen liver sample. This diagnostic approach was selected following the determination of an absence of fluke eggs in feces, as is characteristic of dead-end and aberrant host infections. While impala likely play little role in determining population-scale epidemiological and ecological dynamics of fluke infections, given their dead-end host status, this report serves as an important reference for managers and veterinary professionals hoping to preserve the health and welfare of exotic bovids that interface with native American cervids, the definitive hosts of <em>Fascioloides magna</em>.</div></div>\",\"PeriodicalId\":23600,\"journal\":{\"name\":\"Veterinary parasitology, regional studies and reports\",\"volume\":\"63 \",\"pages\":\"Article 101311\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2025-07-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary parasitology, regional studies and reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405939025001194\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology, regional studies and reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405939025001194","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Fascioloides magna infection in a captive impala (Aepyceros melampus) in Florida, USA
Parasitic diseases are associated with diverse clinical signs, and veterinary professionals must be familiar with the presentations of infections in a variety of hosts. As human activity introduces new and exotic host species, expanding our understanding of pathogen effects in new species becomes integral to effective surveillance, diagnosis, and treatment. This article presents the expansion of the known host range of the American liver fluke, Fascioloides magna, with the infection of a farmed impala (Aepyceros melampus) in Marion County, Florida, USA. The gross and histopathological lesions observed are consistent with previous reports of dead-end host infections, particularly black striping across the peritoneum and abdominal viscera, a prominent pseudocyst on the visceral face of the liver, and hepatic fibrosis and biliary hyperplasia associated with tissue taken from that pseudocyst. While no whole or partial fluke was obtained from a field necropsy of the impala, amplification via cPCR and Sanger sequencing of the ITS2 region confirmed the presence of Fascioloides magna DNA in a frozen liver sample. This diagnostic approach was selected following the determination of an absence of fluke eggs in feces, as is characteristic of dead-end and aberrant host infections. While impala likely play little role in determining population-scale epidemiological and ecological dynamics of fluke infections, given their dead-end host status, this report serves as an important reference for managers and veterinary professionals hoping to preserve the health and welfare of exotic bovids that interface with native American cervids, the definitive hosts of Fascioloides magna.
期刊介绍:
Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).