Veterinaria italiana最新文献_第3页

Investigation of Peste des petits ruminants virus circulation in Uttarakhand, India: a step towards global eradication of PPR by 2030. 印度北阿坎德邦小反刍兽疫病毒传播调查：向 2030 年全球根除小反刍兽疫迈出的一步。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.2988.21721.3

Amisha Netam, Anuj Tewari, Rajesh Kumar

{"title":"Investigation of Peste des petits ruminants virus circulation in Uttarakhand, India: a step towards global eradication of PPR by 2030.","authors":"Amisha Netam, Anuj Tewari, Rajesh Kumar","doi":"10.12834/VetIt.2988.21721.3","DOIUrl":"https://doi.org/10.12834/VetIt.2988.21721.3","url":null,"abstract":"This study was conducted to estimate the seroprevalence of Peste des petits ruminants virus (PPRV) and to determine the virus distribution in unvaccinated goats in the Pantnagar region of Uttarakhand state, India. A total of 212 serum samples from goats were collected randomly from various villages in three districts (Udhamsingh Nagar, Nainital, and Almora) of Uttarakhand. Serum samples were tested for anti-PPRV antibodies by a commercially available kit. RNA was extracted from the clinical samples and it was subjected to one-step RT-PCR, followed by virus isolation from positive samples. A total of 41 animals from various villages were found to be seropositive with a prevalence rate of 19.33%. PPR outbreaks were also reported from the Tarai region of Uttarakhand, and detection by PCR confirmed PPRV in 8 goats. Two representative swab samples were subjected to virus isolation in Vero cells and both samples showed typical cytopathic effects. The present study shows that PPRV is circulating in the Tarai region of Uttarakhand and mass vaccination for PPR must be followed in this region to increase herd immunity to a protective level. To the best of our knowledge, this is the first investigation of PPRV seroprevalence in unvaccinated goats of Uttarakhand, India.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive genetic analysis of the first near-complete genome of bovine coronavirus and partial genome of bovine rotavirus in Türkiye through metagenomics. 通过元基因组学对土耳其首个近乎完整的牛冠状病毒基因组和牛轮状病毒部分基因组进行综合遗传分析。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3372.22817.2

Emel Aksoy, Ahmet Kursat Azkur, İbrahim Halil Miraloglu

{"title":"Comprehensive genetic analysis of the first near-complete genome of bovine coronavirus and partial genome of bovine rotavirus in Türkiye through metagenomics.","authors":"Emel Aksoy, Ahmet Kursat Azkur, İbrahim Halil Miraloglu","doi":"10.12834/VetIt.3372.22817.2","DOIUrl":"https://doi.org/10.12834/VetIt.3372.22817.2","url":null,"abstract":"Obtaining the complete or near-complete genome sequence of pathogens is becoming increasingly crucial for epidemiology, virology, clinical science and practice. This study aimed to detect viruses and conduct genetic characterization of genomes using metagenomics in order to identify the viral agents responsible for a calf's diarrhoea. The findings showed that bovine coronavirus (BCoV) and bovine rotavirus (BRV) are the primary viral agents responsible for the calf's diarrhoea. The current study successfully obtained the first-ever near-complete genome sequence of a bovine coronavirus (BCoV) from Türkiye. The G+C content was 36.31% and the genetic analysis revealed that the Turkish BCoV strain is closely related to respiratory BCoV strains from France and Ireland, with high nucleotide sequence and amino acid identity and similarity. In the present study, analysis of the S protein of the Turkish BCoV strain revealed the presence of 13 amino acid insertions, one of which was found to be shared with the French respiratory BCoV. The study also identified a BRV strain through metagenomic analysis and detected multiple mutations within the structural and non-structural proteins of the BRV strain, suggesting that the BRV Kirikkale strain may serve as an ancestor for reassortants with interspecies transmission, especially involving rotaviruses that infect rabbits and giraffes.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and dynamics of tumor necrosis factor alpha in the diagnosis and treatment of canine heartworm disease. 在犬心丝虫病的诊断和治疗中检测肿瘤坏死因子 alpha 及其动态变化。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.2662.22847.2

Ljubica Spasojević Kosić, Vuk Vracar, Gordana Kozoderovic, Vesna Lalosevic

{"title":"Detection and dynamics of tumor necrosis factor alpha in the diagnosis and treatment of canine heartworm disease.","authors":"Ljubica Spasojević Kosić, Vuk Vracar, Gordana Kozoderovic, Vesna Lalosevic","doi":"10.12834/VetIt.2662.22847.2","DOIUrl":"10.12834/VetIt.2662.22847.2","url":null,"abstract":"The aim of this study was to determine the concentration of TNF-alpha (TNF-α) in dogs naturally infected with Dirofilaria immitis (D. immitis) and to assess whether there are any changes in TNF-α concentration and their dependence during therapy for heartworm disease (HWD). For this study, 14 client-owned dogs with HWD were selected. Clinical and parasitological examinations (modified Knott test for circulating microfilariae and SNAP Test IDEXX for circulating D. immitis antigen) had been used for diagnosing D. immitis and HWD. All dogs were treated with an alternative therapy for HWD (oral doxycycline 10 mg/kg b.w., once daily for 6 weeks, then alternately 4 weeks without and 2 weeks with the medication, and oral ivermectin 6-14 µg/kg b.w., every 2 weeks). The dogs blood sera at the moment of HWD diagnosis, during and at the end of therapy were frozen for further quantifying of TNF-α (Canine TNF-alpha ELISA kit, Thermo scientific). At the moment of HWD diagnosis TNF-α was detected in 9 dogs (7.21±12.44 pg/ml). Concentration of TNF-α was not significantly change during the therapy, neither related to the level of D. immitis antigen nor to antigen level changes. The alternative therapy for HWD has no influence on TNF-α concentration dynamics.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic evaluation of nested PCR and microscopy for Cryptosporidiosis in goats: can COWP gene based qRT-PCR be useful in assessment of active Cryptosporidial infections? 巢式 PCR 和显微镜对山羊隐孢子虫病的诊断评估：基于 COWP 基因的 qRT-PCR 是否有助于评估活动性隐孢子虫感染？

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3056.22638.1

Supriya Sachan, Gururaj Kumaresan, Dinesh Kumar Sharma, Giridhari Das, Suman Kumar, Ravi Khare, Anjali Pachoori, Souvik Paul

{"title":"Diagnostic evaluation of nested PCR and microscopy for Cryptosporidiosis in goats: can COWP gene based qRT-PCR be useful in assessment of active Cryptosporidial infections?","authors":"Supriya Sachan, Gururaj Kumaresan, Dinesh Kumar Sharma, Giridhari Das, Suman Kumar, Ravi Khare, Anjali Pachoori, Souvik Paul","doi":"10.12834/VetIt.3056.22638.1","DOIUrl":"10.12834/VetIt.3056.22638.1","url":null,"abstract":"The present research delved into the transmission patterns, diagnostic methods, molecular traits, and phylogenetic analysis of Cryptosporidium species. The research was undertaken to enhance comprehension of the epidemiology and the potential for zoonotic transmission. A total of 80 goat-kid samples were tested, 7 were confirmed positive by mZN microscopy and 12 by nested-PCR. By PCR, 18SSUrRNA, HSP70, and GP60 amplicons were tested for Cryptosporidium. The restriction enzymes viz., SspI, VspI and MboII were used to genotype 12 Cryptosporidium positive samples by which C. parvum and C. bovis mixed infections were detected. Quantitative reverse transcription real-time PCR was used to transcriptionally screen the COWP-subunit genes to assess the severity of the infection in goat-kids, which showed upregulation of COWP6 and COWP4, while COWP9 and COWP3 genes were downregulated. A silent mutation was found at the codon CCA→CCC, which is being reported for the first time in goat field isolates. Phylogenetic and sequencing analyses confirmed the presence of the anthropozoonotic IIe subtype.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxigenic Staphylococcus aureus in some animal-originated food products marketed in Turkey: presence and Public Health concerns. 土耳其市场上销售的一些动物源性食品中的毒性金黄色葡萄球菌：存在情况和公共卫生问题。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3022.21271.2

Fulden Karadal, Nurhan Ertas Onmaz, Harun Hizlisoy, Yeliz Yildirim, Serhat Al, Zafer Gonulalan, Cemalettin Bagci

{"title":"Toxigenic Staphylococcus aureus in some animal-originated food products marketed in Turkey: presence and Public Health concerns.","authors":"Fulden Karadal, Nurhan Ertas Onmaz, Harun Hizlisoy, Yeliz Yildirim, Serhat Al, Zafer Gonulalan, Cemalettin Bagci","doi":"10.12834/VetIt.3022.21271.2","DOIUrl":"https://doi.org/10.12834/VetIt.3022.21271.2","url":null,"abstract":"This study aimed to detect the presence of Staphylococcus aureus in some animal source food (ASF) including emulsified meat products (sausage and salami), dry fermented meat product (soudjouk), semi dry meat product (pastrami) and raw chicken meat. Sixty six (38.8%) of 170 samples were found to be positive for S. aureus. It was determined that S. aureus was found in 17 (56.6%) salami, 27 (54%) raw chicken meat, 9 (30%) soudjouk, 9 (30%) pastrami, 4 (13.3%) sausage samples. Staphylococcal enterotoxins (SEs) were identified in 5 out of 66 (7.5 %) isolates food matrices including 3 (4.5%) SEA, 2 (3.03%) SEC. The sea and sec genes were detected in 3 (4.5%) of 66 isolates. The results of this study highlight the need to provide suitable control strategies concerning production, sales, and storage to prevent the spread of enterotoxigenic S. aureus isolates in ASF. The key contribution of this study is its revelation of the presence of S. aureus in animal products sold in Turkish local markets, highlighting the potential public health risks associated with animal foods.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of ELISA in raw milk for detection of antibodies to Mycobacterium avium subsp. paratuberculosis in dairy herd. 评估用酶联免疫吸附法在生奶中检测奶牛副结核分枝杆菌抗体的效果。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.2948.18971.1

Silvina Tieri, Karina Cirone, Claudia Morsella, Laura Méndez, Silvia Mundo, Bárbara Fernández, Fernando Paolicchi

{"title":"Evaluation of ELISA in raw milk for detection of antibodies to Mycobacterium avium subsp. paratuberculosis in dairy herd.","authors":"Silvina Tieri, Karina Cirone, Claudia Morsella, Laura Méndez, Silvia Mundo, Bárbara Fernández, Fernando Paolicchi","doi":"10.12834/VetIt.2948.18971.1","DOIUrl":"https://doi.org/10.12834/VetIt.2948.18971.1","url":null,"abstract":"Paratuberculosis (PTBC) is a chronic intestinal disease of animals caused by Mycobacterium avium paratuberculosis (MAP). MAP infection is diagnosed through indirect tests based on the immune response. The aims of this study were to compare the performance of two milk ELISA for the diagnosis of PTBC and to assess the bulk tank milk (BTM) MAP exposure in dairy cattle in Argentina. A total of 357 fecal, serum, and milk samples were collected. The fecal samples were processed by culture for MAP isolation, while both, serum and milk samples were used for the detection of antibodies by two different ELISA tests, \"in-house\" and commercial kit. MAP was isolated in 3.9% of fecal samples. For milk ELISA, poor concordances were obtained. Optimized cut-off points were calculated. The highest sensitivity and specificity values (64% and 80% respectively) were obtained with the combination of MAP isolation and commercial milk ELISA. The results indicate that the combination of different techniques to identify of dairy cattle infected with MAP increases the efficiency of diagnosis. In addition, BTM samples (n=98) were evaluated to determine herd status using the commercial kit during two seasons, identifying 33.3% of positive samples in autumn and 35.4% in spring.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of vesivirus in minks (Neovison vison), Italy 2021. 在水貂（Neovison vison）中检测到囊病毒，意大利 2021 年。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3472.23552.1

Paola Ripà, Francesco Perugini, Nicola Decaro, Valentina Curini, Addolorato Ruberto, Maurilia Marcacci, Vito Martella, Alessio Lorusso, Gianvito Lanave

引用次数: 0

Meat juice as a feasible alternative sample for tuberculosis surveillance in large game. 将肉汁作为监测大型野味中结核病的可行替代样本。

4区农林科学

Veterinaria italiana Pub Date : 2024-03-31 DOI: 10.12834/VetIt.3132.22735.2

Ana Carolina Abrantes, David Risco, Miguel Carvalho, Rosario Cerrato, Pedro Fernández-Llario, Madalena Vieira-Pinto

引用次数: 0

Does a therapeutical dose of ivermectin impairs testicular homeostasis of rats via excessive apoptosis? 治疗剂量的伊维菌素会通过过度凋亡损害大鼠睾丸的稳态吗？

4区农林科学

Veterinaria italiana Pub Date : 2024-02-20 DOI: 10.12834/VetIt.2692.19722.2

Flora Cordeiro, Flaviane L Ceglio, Nathalia A Galvão, Leoni V Bonamin, Eduardo F Bondan, Thiago Berti Kirsten, Maria M Bernardi

{"title":"Does a therapeutical dose of ivermectin impairs testicular homeostasis of rats via excessive apoptosis?","authors":"Flora Cordeiro, Flaviane L Ceglio, Nathalia A Galvão, Leoni V Bonamin, Eduardo F Bondan, Thiago Berti Kirsten, Maria M Bernardi","doi":"10.12834/VetIt.2692.19722.2","DOIUrl":"10.12834/VetIt.2692.19722.2","url":null,"abstract":"Ivermectin is a medication used to treat parasite infestations in humans and in veterinary medicine. Previously we showed that therapeutical doses of ivermectin impaired spermatogenesis and spermiogenesis in adult rats. The present study was proposed to understand the pathophysiological mechanism that triggered these impairments induced by ivermectin. It was a particular objective to study if ivermectin induced excessive apoptosis. Adult rats were treated with a therapeutical dose of ivermectin (subcutaneously). Their testis was evaluated for the expression of caspase-3 (a marker of apoptosis), using immunohistochemistry techniques. Results revealed that ivermectin treatment increased the expression of caspase-3 (labeled seminiferous tubules and strongly labeled tubules), as well as increased the number of tubules that presented labeled cells in the tubular lumen, compared to the data of the control group. In conclusion, a therapeutical dose of ivermectin induced expressive apoptosis in cells of the seminiferous tubules of rats, affecting the testicular natural homeostasis process, which resulted in the spermatogenesis and spermiogenesis impairments previously reported.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139906482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic Resistance Profile of Rarely Isolated Salmonella Serotypes from Poultry in Turkey. 土耳其家禽中罕见沙门氏菌血清型的英语抗生素耐药性概况。

4区农林科学

Veterinaria italiana Pub Date : 2023-12-31 DOI: 10.12834/VetIt.3004.20698.2

Hamit Kaan Müştak, Seyyide Sarıçam İnce, İnci Başak Müştak

{"title":"Antibiotic Resistance Profile of Rarely Isolated Salmonella Serotypes from Poultry in Turkey.","authors":"Hamit Kaan Müştak, Seyyide Sarıçam İnce, İnci Başak Müştak","doi":"10.12834/VetIt.3004.20698.2","DOIUrl":"10.12834/VetIt.3004.20698.2","url":null,"abstract":"This study investigated five strains of each serotype of Salmonella Agona, Salmonella Heidelberg, Salmonella Hindmarsh, Salmonella Kouka, Salmonella Muenchen, Salmonella Ottmarchen, Salmonella Saintpaul and Salmonella II, isolated during the 2014-2017 period. Disc diffusion was used to identify the phenotypic profiles of antibiotic resistance to 12 antimicrobials while the presence of antibiotic resistance genes (ARGs) was detected by PCR. The most sensitive serotype was S. Kouka while the most resistant serotypes were S. Agona and S. Heidelberg. MDR was detected most frequently in S. Agona strains, followed by S. Saintpaul, S. Hindmarsch, and S. Ottmarchen. The samples were most susceptible to chloramphenicol and ceftazidime and most resistant to sulfonamide. The resistance genes were detected in phenotypically resistant strains. Among the tetracycline-resistant strains, tet (A) was the most prevalent gene. The results of this study highlight the importance of monitoring antibiotic resistance profiles and related genes, which can spread to form MDR bacteria. Salmonella spp., which significantly contribute to ARG dissemination, should be monitored constantly to protect the closely related health of humans, animals, and the environment. The level of antibiotic resistance observed in this study, even in rarely isolated Salmonella serotypes, also indicates the need for careful and selective use of antibiotics.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0