Veterinaria italiana最新文献

Insights into Bovine Viral Diarrhea Epidemiology in Moroccan Cattle Population. 摩洛哥牛群病毒性腹泻流行病学研究。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-06-23 DOI: 10.12834/VetIt.3604.32125.2

Hanane Khallouki, Fatiha El Mellouli, Youssef Edderdouri, Oumar Konate, Houria Abdelghaffar

{"title":"Insights into Bovine Viral Diarrhea Epidemiology in Moroccan Cattle Population.","authors":"Hanane Khallouki, Fatiha El Mellouli, Youssef Edderdouri, Oumar Konate, Houria Abdelghaffar","doi":"10.12834/VetIt.3604.32125.2","DOIUrl":"10.12834/VetIt.3604.32125.2","url":null,"abstract":"This study aimed to estimate the seroprevalence of bovine viral diarrhea virus (BVDV) in Moroccan cattle and to investigate associations with age, sex, breed, housing system, and geographic region. A total of 545 serum samples were randomly collected from cattle across various Moroccan regions between December 2023 and February 2024. Antibodies against BVDV were detected using a commercial competitive ELISA, and herd-level data were gathered via questionnaire. Overall, 25.0% (95% CI: 21.4 - 28.8) of samples tested seropositive. The highest prevalence was observed in the Tanger-Tetouan-Al Hoceima region (44.0%; 95% CI: 24.4 - 65.1), followed by Casablanca-Settat (32.6%; 95% CI: 26.1 - 39.6). Seropositivity was highest among cattle older than 12 months (37.5%) and in crossbred animals (86.1%) compared to purebreds (74.3%). Although not statistically significant, cattle in intensive systems showed higher prevalence (29.3%) than those in semi-intensive systems (23.2%). Females exhibited a higher seroprevalence (27.87%) than males (21.7%), with sex emerging as a significant risk factor. These findings highlight the widespread circulation of BVDV in Morocco and underscore the need for targeted control measures and improved herd management practices to mitigate virus transmission.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 3","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neospora caninum in Cattle Herds: Risk Factors, Prevalence and Molecular Characterization in Western Türkiye. 牛群中的犬新孢子虫：危险因素、流行率和分子特征。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-06-20 DOI: 10.12834/VetIt.3720.34145.2

Ömer Faruk Gökcecik, Hasan Eren

{"title":"Neospora caninum in Cattle Herds: Risk Factors, Prevalence and Molecular Characterization in Western Türkiye.","authors":"Ömer Faruk Gökcecik, Hasan Eren","doi":"10.12834/VetIt.3720.34145.2","DOIUrl":"10.12834/VetIt.3720.34145.2","url":null,"abstract":"Neosporosis is a major cause of bovine abortion worldwide, leading to substantial economic losses. In western Türkiye, an area characterized by intensive cattle breeding, data on this disease remain limited, and no prior studies have investigated its risk factors. This study aimed to determine the prevalence, associated risk factors, and phylogenetic profile of Neospora caninum in the region. Visceral tissues from 88 aborted cattle fetuses were analyzed through genomic DNA isolation, real-time PCR (qPCR), and sequencing. In parallel, the presence of N. caninum antibodies was assessed in 973 serum samples using ELISA. N. caninum DNA was detected in 8 (9.09%) fetal samples by qPCR, with three of these further confirmed via conventional PCR and sequencing. Sequence analysis validated the presence of N. caninum in these three samples. Serologically, 122 of 973 serum samples (12.53%) tested positive for N. caninum antibodies. Statistical analysis of potential risk factors-including province, gender, age group, origin, and farm type-identified farm type as the most significant determinant of seropositivity. Notably, family-operated farms exhibited a higher prevalence of positive cases. This study represents the first large-scale investigation of N. caninum-associated reproductive losses in western Türkiye. The findings offer valuable insights for the development of targeted control and prevention strategies in affected cattle populations.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 3","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insight of avian reovirus circulating among desi-chickens in Tamil Nadu, South India. 南印度泰米尔纳德邦鸡群中传播的呼肠孤病毒的基因组分析。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-06-20 DOI: 10.12834/VetIt.3760.34630.2

M Pranay, A Balasubramaniam, P Ponnusamy, P Balachandran, K Sukumar

{"title":"Genomic insight of avian reovirus circulating among desi-chickens in Tamil Nadu, South India.","authors":"M Pranay, A Balasubramaniam, P Ponnusamy, P Balachandran, K Sukumar","doi":"10.12834/VetIt.3760.34630.2","DOIUrl":"10.12834/VetIt.3760.34630.2","url":null,"abstract":"Avian reovirus (ARV) is a major causative agent of viral arthritis (VA), tenosynovitis, and malabsorption syndrome (MAS) in chickens, with significant economic consequences due to growth retardation, reduced production performance, and immunosuppression. Despite routine vaccination of breeder chickens against ARV, cases of VA and MAS continue to be reported in commercial flocks in recent years. Moreover, there is a lack of recent data on the genetic characteristics of circulating field ARV strains in India. In light of these concerns, a study was conducted to investigate the involvement of ARV in chickens exhibiting clinical signs suggestive of VA or MAS. Samples were collected from 27 commercial broiler and desi-chicken flocks across the mid-western region of Tamil Nadu, South India. Molecular confirmation was performed using reverse transcription polymerase chain reaction (RT-PCR) targeting a partial region of the σC gene within the S1 segment. Of the 27 flocks sampled, only two samples - both from desi-chickens aged two and three weeks - tested positive for ARV. Sequence analysis of these positive samples, compared against available ARV sequences in GenBank (including vaccine strains) revealed that the identified strains clustered within ARV genogroup II. This represents the first report of cluster II ARV in India, indicating the circulation of genetically distinct ARV strains in Indian poultry populations. These findings underscore the need for routine molecular surveillance of ARV genotypes in India and highlight the potential mismatch between circulating field strains and current vaccine strains. Comprehensive genotype monitoring is essential to upgrade vaccine design and implement effective control strategies for ARV-associated diseases in Indian poultry production.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 3","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing Cadmium Levels in Horses Imported from the European Union and Slaughtered in Italy. 评估从欧盟进口并在意大利屠宰的马的镉含量。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-04-14 DOI: 10.12834/VetIt.3692.31893.2

Michela Maria Dimuccio, Edmondo Ceci, Elisabetta Bonerba, Francesco Emanuele Celentano, Pasquale De Marzo, Rosa Leone, Stefano Sportelli, Giancarlo Bozzo

{"title":"Assessing Cadmium Levels in Horses Imported from the European Union and Slaughtered in Italy.","authors":"Michela Maria Dimuccio, Edmondo Ceci, Elisabetta Bonerba, Francesco Emanuele Celentano, Pasquale De Marzo, Rosa Leone, Stefano Sportelli, Giancarlo Bozzo","doi":"10.12834/VetIt.3692.31893.2","DOIUrl":"https://doi.org/10.12834/VetIt.3692.31893.2","url":null,"abstract":"The aim of this study was to evaluate cadmium (Cd) concentrations in horses imported from various European Union countries and slaughtered in Italy. In addition, we assessed the potential correlation between Cd levels in tissues and organs, and Cd-related gross and histological lesions. The animals were divided into six groups based on age and geographical origin. Among the 430 animals examined, gross renal lesions were observed in only two kidney samples from Polish horses, which also had the highest Cd concentrations: 9.1 mg/kg w/w and 8.23 mg/kg w/w, respectively. Further histological alterations were identified in the same group, in 19 kidney samples with Cd concentrations exceeding 5.72 mg/kg w/w. These findings raise important food safety concerns, as Regulation (EC) No. 1881/2006 and its subsequent amendments establish strict maximum limits for Cd in horse meat and offal. Considering the EU precautionary principle, the results of this study underscore that only the integration of chemical analyses with histological examinations can provide a comprehensive assessment of the risks, ensuring compliance with EU food safety legislation and international trade standards.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotyping and antibiotic susceptibility of Salmonella strains collected from sheep and cattle samples in Algeria. 从阿尔及利亚羊和牛样本采集的沙门氏菌的基因分型和抗生素敏感性。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-04-14 DOI: 10.12834/VetIt.3609.32058.2

Siham Nouichi, Lynda Mezali, Taha Mossadak Hamdi, Christian Vinicio Vinueza-Burgos, Lieven De Zutter

{"title":"Genotyping and antibiotic susceptibility of Salmonella strains collected from sheep and cattle samples in Algeria.","authors":"Siham Nouichi, Lynda Mezali, Taha Mossadak Hamdi, Christian Vinicio Vinueza-Burgos, Lieven De Zutter","doi":"10.12834/VetIt.3609.32058.2","DOIUrl":"https://doi.org/10.12834/VetIt.3609.32058.2","url":null,"abstract":"The present work investigates the genetic relatedness and antibiotic susceptibility of Salmonella strains collected from the red meat supply chain, highlighting the public health significance of these pathogens. Pulsed-field gel electrophoresis (PFGE-XbaI) was applied to genotype a collection of 84 Salmonella strains isolated from slaughterhouses. The antibiotic susceptibility of these strains to fourteen antimicrobial agents was determined using the minimum inhibitory concentration (MIC) method. The isolates were classified into 22 fingerprints, with two strains being non-typable. The predominant PFGE types identified were Mu1 (n=18), I2 (n=10), and K2 (n=8), indicating a high level of genetic similarity among isolates (>80%). All Salmonella strains exhibited resistance to at least two antimicrobials, with approximately 34.5% displaying resistance to three or more classes of antibiotics. Twelve distinct resistant patterns were identified, and notably, only one colistin-resistant Salmonella strain was detected. These findings underscore the need for ongoing surveillance and control measures in the red meat industry.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serosurvey of Bluetongue virus in small ruminants in Egypt and its associated risk factors. 埃及小反刍动物蓝舌病病毒血清调查及其相关危险因素

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-04-14 DOI: 10.12834/VetIt.3610.31142.3

Abdelfattah Selim, Mohamed Marzok, Hattan S Gattan, Mohammed Ali Al-Hammadi

{"title":"Serosurvey of Bluetongue virus in small ruminants in Egypt and its associated risk factors.","authors":"Abdelfattah Selim, Mohamed Marzok, Hattan S Gattan, Mohammed Ali Al-Hammadi","doi":"10.12834/VetIt.3610.31142.3","DOIUrl":"https://doi.org/10.12834/VetIt.3610.31142.3","url":null,"abstract":"Bluetongue is an emerging, non-contagious, vector-borne disease that affects both domestic and wild ruminants. This study aimed to determine the seroprevalence of Bluetongue virus (BTV) in four Egyptian governorates and to evaluate the associated risk factors. A total of 740 serum samples were collected from 380 sheep and 360 goats and tested using a commercial competitive ELISA (cELISA). The overall BTV seroprevalence was 16.2%, with 17.1% in sheep and 15.3% in goats. Although the seroprevalence did not differ significantly across the studied regions, the highest prevalence was recorded in Kafr El-Sheikh (20.7%). Univariable analysis revealed a significant association between BTV seropositivity and several factors, including sex, age, presence of vectors, history of abortion, and contact with cattle. According to the multivariable logistic regression model, females, animals older than 2 years, and those with a history of abortion were respectively 2.3, 2.6, and 1.6 times more likely to be seropositive. Furthermore, the presence of insect vectors and close contact with cattle increased the risk of BTV infection by 1.6 and 2.1 times, respectively. This study highlights the significant risk factors associated with BTV seropositivity, with a slightly higher prevalence observed in sheep compared to goats. These findings underscore the need for effective disease surveillance, management, and control strategies targeting both sheep and goat populations.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative effectiveness of different biological specimens for PRRSv detection in naturally infected fattening pigs. 不同生物标本对自然感染育肥猪PRRSv检测效果的比较。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-04-14 DOI: 10.12834/VetIt.3650.31701.2

Milena Monnier, Silvia Marro, Andrea Trossi, Marco Faccenda, Nicoletta Vitale, Roberto Zoccola, Ilaria Miceli, Francesca Rossi, Simone Peletto, Alessandro Dondo, Mariella Goria

{"title":"Comparative effectiveness of different biological specimens for PRRSv detection in naturally infected fattening pigs.","authors":"Milena Monnier, Silvia Marro, Andrea Trossi, Marco Faccenda, Nicoletta Vitale, Roberto Zoccola, Ilaria Miceli, Francesca Rossi, Simone Peletto, Alessandro Dondo, Mariella Goria","doi":"10.12834/VetIt.3650.31701.2","DOIUrl":"https://doi.org/10.12834/VetIt.3650.31701.2","url":null,"abstract":"One of the most critical aspects of PRRS outbreak control in swine herds is the reliable virus detection in both newly introduced animals and replacement gilts. In present study we compared the effectiveness of different biological specimens which - alone or in combination - allow to detect PRRSv carrier animals by mean of Reverse Transcriptase nested PCR (RT-nPCR). Five different matrices (serum, nasal swab, oral fluid, tracheobronchial swab and bronchoalveolar lavage fluid - BALF), known to be effective for PRRSv detection, were included in diagnostic efficacy comparison. Thirty-five pigs newly introduced into a fattening unit of a PRRS chronically affected farm were randomly selected to be submitted to serial sampling of each of the matrices above described, during the first three months of fattening period. A Bayesian approach was used to analyze the RT-nPCR results (i.e., positive or negative) of each sampling method and their respective sensitivity was estimated. PRRSv was detected by RT-nPCR in at least one matrix from 58% to 100% of the pigs. Tracheobronchial swabbing, as well as the combination of tracheobronchial swabbing plus bronchoalveolar washing, or tracheobronchial swabbing plus serum sampling were proved to be the most sensitive sampling methods to detect PRRSv in naturally infected live pigs. This study enlightens as the tracheobronchial swabbing associated with RT-nPCR could be the most recommended diagnostic tool for assessing infection dynamics in pig herds.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of the Prion Protein Gene in the indigenous Nguni cattle breed of the Kingdom of Eswatini. 斯瓦蒂尼王国土著恩古尼牛品种朊蛋白基因的遗传特征。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-04-14 DOI: 10.12834/VetIt.3560.26921.2

Pumla Bhekiwe Manyatsi, Sedef Erkunt Alak, Hüseyin Can, Ahmet Efe Köseoğlu, Mervenur Güvendi, Cemal Ün

{"title":"Genetic characterization of the Prion Protein Gene in the indigenous Nguni cattle breed of the Kingdom of Eswatini.","authors":"Pumla Bhekiwe Manyatsi, Sedef Erkunt Alak, Hüseyin Can, Ahmet Efe Köseoğlu, Mervenur Güvendi, Cemal Ün","doi":"10.12834/VetIt.3560.26921.2","DOIUrl":"https://doi.org/10.12834/VetIt.3560.26921.2","url":null,"abstract":"Bovine spongiform encephalopathy (BSE) is one of several fatal neurodegenerative diseases caused by misfolded, infectious prion proteins. Polymorphisms in the prion protein gene (PRNP) associated with BSE susceptibility have been reported in various cattle breeds. However, no studies have been conducted to investigate these genetic variations in the Nguni cattle breed, an indigenous breed in the Kingdom of Eswatini, Southern Africa. The present study aimed to genetically characterize the PRNP gene in Nguni cattle. Both the coding region and regulatory elements-specifically the promoter and intron 1 regions-were analyzed. Three synonymous polymorphisms were identified: Q78Q, P113P, and I226I. Additionally, the Nguni breed exhibited a higher frequency of deletion alleles in two known insertion/deletion (indel) polymorphisms: a 23-bp deletion in the promoter region and a 12-bp deletion in intron 1, compared to insertion alleles. These findings suggest that Nguni cattle may be genetically susceptible to BSE. The results underscore the importance of implementing selective breeding strategies aimed at enhancing resistance to BSE within this indigenous cattle population.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of faecal prevalence of internal parasite eggs in children and dogs from three rural high-altitude hamlets in the Peruvian northern Andes. 秘鲁北部安第斯山脉三个农村高海拔村庄儿童和狗粪便中寄生虫卵的流行情况调查。

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-04-14 DOI: 10.12834/VetIt.3515.24186.2

Jennifer Lerida Cuzcano Anarcaya, Luis Vargas-Rocha, Jierson E Mendoza-Estela, María Cabrera

{"title":"Exploration of faecal prevalence of internal parasite eggs in children and dogs from three rural high-altitude hamlets in the Peruvian northern Andes.","authors":"Jennifer Lerida Cuzcano Anarcaya, Luis Vargas-Rocha, Jierson E Mendoza-Estela, María Cabrera","doi":"10.12834/VetIt.3515.24186.2","DOIUrl":"https://doi.org/10.12834/VetIt.3515.24186.2","url":null,"abstract":"This study aimed to identify and determine the prevalence of intestinal parasites through faecal egg detection in children and dogs from three rural hamlets - La Chorrera, Cruz Pampa, and Chugurmayo - located in the Sorochuco district, Celendín province, Cajamarca region, Peru, at an altitude exceeding 3,400 meters above sea level. Faecal samples were collected from school-aged children and domestic dogs and analysed using direct smear, natural sedimentation, Faust, and Sheather techniques. Parasitic infection was detected in 59.80% of children (95% CI: 50.29-69.32) and 55.00% of dogs (95% CI: 44.10-65.90). No statistically significant differences in prevalence were observed between hamlets (p = 0.630). Among children, the highest prevalence was noted in males aged >6 to 9 years; in dogs, it was observed in males aged 0 to 1 year. However, age and sex were not significantly associated with infection in either group (p > 0.05). Parasitic stages identified in children included Giardia spp., Hymenolepis spp., Fasciola hepatica, Ascaris lumbricoides, and Diphyllobothrium spp. In dogs, Giardia spp., Ancylostomatidae, Sarcocystis spp., Toxocara spp., and Taenia spp. were found. One parasite - Giardia spp. - were common to both species, although no significant overall correlation of infection between children and dogs was observed (p > 0.05). These findings highlight the need for enhanced epidemiological surveillance, medical attention, and further research in these underserved rural communities. Increased awareness and integrated public health efforts are essential to address the burden of parasitic infections in these high-altitude areas.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and Characterization of Egg-Derived Antibodies (IgY) Against Outer Membrane Proteins of Multidrug-Resistant Salmonella enterica Serovar Typhimurium. 抗多药耐药肠炎沙门氏菌血清型鼠伤寒沙门氏菌外膜蛋白的蛋源抗体（IgY）的制备与鉴定

IF 0.5 4区农林科学

Veterinaria italiana Pub Date : 2025-04-14 DOI: 10.12834/VetIt.3565.31552.3

Aakanksha Tiwari, Rajesh Kumar, Garima Pandey, Namita Shukla, Anupama Mishra, Anuj Tewari

{"title":"Production and Characterization of Egg-Derived Antibodies (IgY) Against Outer Membrane Proteins of Multidrug-Resistant Salmonella enterica Serovar Typhimurium.","authors":"Aakanksha Tiwari, Rajesh Kumar, Garima Pandey, Namita Shukla, Anupama Mishra, Anuj Tewari","doi":"10.12834/VetIt.3565.31552.3","DOIUrl":"https://doi.org/10.12834/VetIt.3565.31552.3","url":null,"abstract":"Non-typhoidal salmonellosis is a globally prevalent zoonotic disease, often associated with the growing concern of antimicrobial resistance (AMR). This persistent issue underscores the urgent need for novel strategies for prevention and control. Although conventional approaches such as drug or vaccine development are widely explored, they are often time-consuming, costly, and subject to potential bacterial resistance over time. As an alternative, egg-derived antibodies (IgY) offer a promising solution. IgY can be produced in large quantities without the need for complex infrastructure or environmental risk. Their production is characterized by a favorable safety profile, scalability, high antibody yield per egg yolk, cost-effectiveness, and lack of cross-reactivity in mammals-attributes that collectively make this technology highly advantageous. The present investigation aimed to establish a standardized protocol for hyperimmunization, production, and isolation of IgY antibodies from the yolk of immunized hens targeting multidrug-resistant (MDR) Salmonella enterica serovar Typhimurium. The study further focused on the characterization and specificity assessment of the purified IgY. The findings suggest that the resulting IgY holds strong potential as a therapeutic, prophylactic, or diagnostic agent against MDR S. Typhimurium.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 2","pages":""},"PeriodicalIF":0.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0