Detection and molecular characterization of the BEF virus in western Türkiye.

IF 0.7 4区农林科学 Q4 VETERINARY SCIENCES

Veterinaria italiana Pub Date : 2025-09-22 DOI:10.12834/VetIt.3810.35777.2

Kemal Pekmez, Murat Kaplan, Abdurrahman Anıl Çağırgan, Fatih Arslan

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引用次数: 0

Abstract

Bovine ephemeral fever (BEF) is characterized by high fever, nasal and eye discharge, excessive salivation, muscle weakness, yield losses and or with high morbidity and low mortality. The first epidemic of BEFV in Turkey occurred in 1985 since then epidemics were reported every 2-4 years in the south and southeastern regions of Turkey. Since the first detection of the virus in Turkey, the BEF virus was reported from other parts of the country except the Aegean Region. In November 2020, the possible outbreaks of BEF were reported from two different locations of the Aegean Region in Turkey. In this study, it was aimed to determine the molecular characterization and possible origin of the virus that caused the epidemic in the Aegean Region. For this purpose, blood samples collected from clinically infected animals were tested by RT-qPCR, and complete G gene sequences were carried out of the positive sample using the primers designed in this study. According to the phylogenetic analysis, virus is located in the Middle East lineage. Based on field observations and the data obtained in the study, it was thought that the spread of the virus to the Aegean Region was caused by animal movements from other regions.

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西部基耶病毒（BEF）的检测及分子特征分析。

牛短暂热（BEF）的特征是高热、鼻和眼分泌物、大量流涎、肌肉无力、产量损失和/或发病率高、死亡率低。土耳其的首次BEFV流行发生在1985年，此后在土耳其南部和东南部地区每2-4年报告一次流行。自在土耳其首次发现该病毒以来，除爱琴海地区外，该国其他地区也报告了BEF病毒。2020年11月，在土耳其爱琴海地区的两个不同地点报告了可能发生的BEF疫情。在这项研究中，目的是确定导致爱琴海地区流行的病毒的分子特征和可能的来源。为此，采用RT-qPCR对临床感染动物采集的血液样本进行检测，并利用本研究设计的引物对阳性样本进行完整的G基因测序。根据系统发育分析，该病毒属于中东谱系。根据实地观察和研究中获得的数据，人们认为，该病毒向爱琴海地区的传播是由来自其他地区的动物运动引起的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinaria italiana VETERINARY SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.