Veterinaria italiana最新文献_第10页

Epidemiological investigation on Bovine alphaherpesvirus 1 and bovine viral diarrhea virus in cattle and camels in southern Egypt. 埃及南部牛和骆驼中牛甲疱疹病毒1型和牛病毒性腹泻病毒的流行病学调查。

4区农林科学

Veterinaria italiana Pub Date : 2022-12-31 DOI: 10.12834/VetIt.2361.14459.2

Hassan Mahmoud, Alsagher Ali

{"title":"Epidemiological investigation on Bovine alphaherpesvirus 1 and bovine viral diarrhea virus in cattle and camels in southern Egypt.","authors":"Hassan Mahmoud, Alsagher Ali","doi":"10.12834/VetIt.2361.14459.2","DOIUrl":"https://doi.org/10.12834/VetIt.2361.14459.2","url":null,"abstract":"In this study, the ELISA procedure was used to detect antibodies against bovine viral diarrhea (BVDV) and infectious bovine rhinotracheitis (IBRV) viruses. The BVDV serological survey in Aswan province in southern Egypt was carried out on 184 unvaccinated cattle and camels. The overall seroprevalence was 18.48% (34/184), but in cattle was 34.78% (32/92) and 2.18% (2/92) in camels. The serological survey was conducted on infectious bovine rhinotracheitis virus (IBRV) antibodies was conducted on 460 unvaccinated cattle from three different provinces (Qena, Luxor, and Aswan). The overall seroprevalence was 60.00% (276/460). The infection rate in Aswan was higher (83.70%) than Qena and Luxor, 53.63% and 45.65%, respectively. Epidemiological status was established to clarify the influence of location in Qena, Luxor and Aswan for bovine viral diarrhea and infectious bovine rhinotracheitis and the effect of management systems in infection rate for cattle. The high predominance of antibodies in cattle may be the principal factor to limit the cattle industry in Egypt. This study aims to investigate the seroprevalence rate of Bovine alphaherpesvirus 1 and bovine viral diarrhea in cattle and camel in southern Egypt.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9610715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and molecular characterization of typhoidal and non-typhoidal Salmonella isolated from meat and environmental samples of retail shops of Lahore Punjab, Pakistan. 从巴基斯坦拉合尔旁遮普零售商店的肉类和环境样本中分离的伤寒和非伤寒沙门氏菌的流行率和分子特征

4区农林科学

Veterinaria italiana Pub Date : 2022-12-31 DOI: 10.12834/VetIt.2392.14037.1

Shakera Sadiq, Mamoona Chaudhry, Muhammad Hassan Mushtaq, Junaid Sadiq, Saima Hasan, Lettini Antonia Anna

{"title":"Prevalence and molecular characterization of typhoidal and non-typhoidal Salmonella isolated from meat and environmental samples of retail shops of Lahore Punjab, Pakistan.","authors":"Shakera Sadiq, Mamoona Chaudhry, Muhammad Hassan Mushtaq, Junaid Sadiq, Saima Hasan, Lettini Antonia Anna","doi":"10.12834/VetIt.2392.14037.1","DOIUrl":"https://doi.org/10.12834/VetIt.2392.14037.1","url":null,"abstract":"Non-typhoidal Salmonellae are important foodborne bacterial pathogens that can cause bacteremia, gastroenteritis, and subsequent infection. The aim of the study was to determine the prevalence of Salmonella in the live bird market and retail shops of Lahore (Pakistan). A total of 720 samples of chicken meat, chopping board, cages, hands, and transportation vans were collected. Salmonella was recovered from 103 (14.36%) samples. The highest prevalence (33.33%) was found in transportation van samples followed by chicken meat samples (17.26%). In the towns of Lahore, the highest prevalence was found in Samanabad Town (19%) followed by Data Ganj Bakhsh Town (17%) with the lowest in Gulberg Town (6.9%). Salmonella Typhimurium was most common (35.92%) followed by S. Enteritidis (25.24%), S. Dublin (14.56%), S. Gallinarum biovar Gallinarum (8.74%), and untyped Salmonella species (15.53%). This was the first baseline study of the prevalence of non-typhoidal Salmonella at the live bird market and retail shops of Lahore. Implementation of appropriate control measures is required at both the human side and poultry food production chain to reduce the burden and transmission of the zoonotic Salmonellae.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9617052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TF-Test techniques for the laboratory diagnosis of gastrointestinal parasites of humans and animals 用于人和动物胃肠道寄生虫实验室诊断的tf检测技术

4区农林科学

Veterinaria italiana Pub Date : 2022-12-30 DOI: 10.12834/VetIt.2040.10928.1

Sandra Valéria Inácio, Jancarlo Ferreira Gomes, Alexandre Xavier Falcão, Débora Regina Romualdo Da Silva, Walter Bertequini Nagata, Bianca Martins Dos Santos, Felipe Augusto Soares, Saulo Hudson Nery Loiola, Aline do Nascimento Benitez, Stefani Laryssa Rosa, Katia Denise Saraiva Bresciani

{"title":"TF-Test techniques for the laboratory diagnosis of gastrointestinal parasites of humans and animals","authors":"Sandra Valéria Inácio, Jancarlo Ferreira Gomes, Alexandre Xavier Falcão, Débora Regina Romualdo Da Silva, Walter Bertequini Nagata, Bianca Martins Dos Santos, Felipe Augusto Soares, Saulo Hudson Nery Loiola, Aline do Nascimento Benitez, Stefani Laryssa Rosa, Katia Denise Saraiva Bresciani","doi":"10.12834/VetIt.2040.10928.1","DOIUrl":"https://doi.org/10.12834/VetIt.2040.10928.1","url":null,"abstract":"Intestinal parasites inhabit the intestinal tract of humans and animals, causing damages whose severity depends on several factors related to the parasite and the host. Immunocompromised individuals are more likely to develop severe forms of parasitic infestation. The diagnosis of the gastrointestinal parasitosis is mainly performed by the examination of the feces, which consists of the direct visualization and identification of the parasites eliminated through the feces. These tests are generally low sensitive and the microscope slides contain a large number of impurities, which can impair the result of the diagnosis. In order to improve the diagnostic accuracy, a new parasitological technique called Three Fecal Test (TF‑Test) was developed. To further improve its diagnostic accuracy, few modifications of the original protocols have been made with the years. In this study the performance of these new techniques to detect gastrointestinal parasites in human and animal fecal samples was described and discussed in\u0000relation to the performance of other conventional coprological tests. It could be concluded that the TF‑Test conventional and modified can be used for the diagnosis of several human and animal parasites, with satisfactory results.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10637656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multidrug resistant enterohaemorrhagic Escherichia coli serogroups in the faeces of hunted Wildlife, Abeokuta, Nigeria. 尼日利亚阿贝奥库塔被猎杀野生动物粪便中的多重耐药肠出血性大肠杆菌血清群。

4区农林科学

Veterinaria italiana Pub Date : 2022-12-30 DOI: 10.12834/VetIt.1990.12087.2

Olufemi Ernest Ojo, Elizabeth Adesola Amosun, Oluwadaisi Oluwaseyi Opebiyi, Mufutau Atanda Oyekunle, Morenike Atinuke Dipeolu, Ebenezer Babatunde Otesile

{"title":"Multidrug resistant enterohaemorrhagic Escherichia coli serogroups in the faeces of hunted Wildlife, Abeokuta, Nigeria.","authors":"Olufemi Ernest Ojo, Elizabeth Adesola Amosun, Oluwadaisi Oluwaseyi Opebiyi, Mufutau Atanda Oyekunle, Morenike Atinuke Dipeolu, Ebenezer Babatunde Otesile","doi":"10.12834/VetIt.1990.12087.2","DOIUrl":"https://doi.org/10.12834/VetIt.1990.12087.2","url":null,"abstract":"Wildlife play significant roles in the dissemination and zoonotic transmission of pathogens. The enterohaemorrhagic Escherichia coli (EHEC) are associated with complicated cases of food-borne illnesses. This study investigated the presence of EHEC serogroups (O26, O45, O103, O145, O91, O111, O128, O121 and O157) in wildlife species: cane rats (Thryonomys swinderianus), royal antelope (Neotragus pygmaeus), African giant rats (Cricetomys gambianus) and waterbuck (Kobus ellipsiprymnus). EHEC and non-EHEC isolates from these wildlife sources were tested for susceptibility to antimicrobial agents. Overall, 127 (83.0 %) of 153 samples yielded E. coli. Nine (5.9%) samples were positive for EHEC belonging to three serogroups as follows; O26 (n=2), O111 (n=2) and O103 (n=5). The EHEC isolates were from cane rats (n=6) and royal antelope (n=3) and possessed virulence-associated genes stx1 (77.8%), stx2 (100.0%), eaeA (100.0%) and hlyA (100.0%). Overall, 127 E. coli isolates showed resistance to ampicillin (99.2%), ceftiofur (90.6%), tetracycline (90.0%), cephalexin (87.4%), cefotaxime (50.4%), streptomycin 42.5%, ceftazidime (41.7%), nalidixic acid (37.0%), ciprofloxacin (43.6%), amoxicillin/clavulanic acid (32.3%), gentamicin (27.6%), sulphamethoxazole/trimethoprim (25.2%), norfloxacin (17.3%) and chloramphenicol (11.0%). The roles of wildlife in the dissemination and transmission of antimicrobial resistant and zoonotic bacteria should not be neglected for effective preventive and control strategies.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10815388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of paratuberculosis (Johne's disease) in livestock farms of river buffaloes (Bubalus Bubalis) in Nineveh, Iraq. 伊拉克尼尼微河水牛(Bubalus Bubalis)养殖场首次报告副结核(约翰氏病)。

4区农林科学

Veterinaria italiana Pub Date : 2022-12-30 DOI: 10.12834/VetIt.1866.9913.1

Abdulwahed Ahmed Hassan, Mohammed Rahawy, Layth Mahmoud Alkattan, Izhar U H Khan, Amir Abdulmawjood, Michael Bülte

{"title":"First report of paratuberculosis (Johne's disease) in livestock farms of river buffaloes (Bubalus Bubalis) in Nineveh, Iraq.","authors":"Abdulwahed Ahmed Hassan, Mohammed Rahawy, Layth Mahmoud Alkattan, Izhar U H Khan, Amir Abdulmawjood, Michael Bülte","doi":"10.12834/VetIt.1866.9913.1","DOIUrl":"https://doi.org/10.12834/VetIt.1866.9913.1","url":null,"abstract":"The present study was designed to investigate Mycobacterium avium subsp. paratuberculosis (MAP) in dairy buffalo herds from six different geographical areas in Nineveh, Iraq. A total of 87 individual faecal samples from river buffaloes, representing 12 dairy herds, were investigated\u0000for detection of MAP using cultural, Ziehl‑Neelsen and MAP‑specific PCR‑based methods. Overall, MAP was detected at a higher frequency at herd‑level (4/12; 33%) compared to the total individual faecal samples (14/87; 16%) with a cell density ranging from 101 to 103 CFU g‑1. A significantly (p < 0.05) higher frequency (9/17; 53%) of MAP was observed in faecal samples collected from clinically diseased as compared to healthy (5/70; 7%) buffaloes selected for the study. However, no statistically significant difference (p ≥ 0.05) was observed in the frequency of MAP occurrence between clinical (9; 64%) and apparently healthy (5; 36%) cases. This report, which is the first MAP study based on data from Iraqi dairy buffalo herds suggests that MAP transmission is a significant health risk for grazing livestock. In conclusion, this study would help farm owners and regulatory authorities to realise the importance of developing and applying best farm management practices in order to prevent transmission of MAP to healthy animals and the environment. In addition, effective diagnostic tests should be taken into account when carrying out the screening tests.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10637658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of fear and fear evoking stimuli in a population of domestic dogs in Iran: A questionnaire-based study. 伊朗一群家犬的恐惧和恐惧刺激研究:一项基于问卷的研究。

4区农林科学

Veterinaria italiana Pub Date : 2022-12-30 DOI: 10.12834/VetIt.2567.15853.2

Mohammad Amir Qiasvand, Samad Alimohammadi, Shahin Hassanpour

{"title":"Study of fear and fear evoking stimuli in a population of domestic dogs in Iran: A questionnaire-based study.","authors":"Mohammad Amir Qiasvand, Samad Alimohammadi, Shahin Hassanpour","doi":"10.12834/VetIt.2567.15853.2","DOIUrl":"https://doi.org/10.12834/VetIt.2567.15853.2","url":null,"abstract":"General and social acceptance, condition, and rules for dog ownership in Iran are different from western countries. So, this phenomenon leads dissimilar fear and fear evoking stimuli in dogs. So, stress and fear‑related factors and dog's behavioral problems is not fully studied in Iran. Thus, the aim of the current study was to measuring fear‑related factors in dogs in Iran. In the first part of this study demographic information of the owners and dogs were collected using questionnaire from clinics and veterinary hospitals in Tehran. In the second part behavioral tests was done. Questionnaire data were recorded based on 4 items as neuroticism, fear caused by other dogs, fear caused by human and separation behavior. The social contact test was used to determine dog's experience for greeting, cooperation, and handling. According to the results, 69.3% of the dog owners were women and 30.7% men. 84.1% of the dogs were small breeds and 15.9% large‑breed dogs. 63.6% of the owner's complained for impact of laws and prohibitions on their dogs' access to outdoor. The score for greeting, cooperation and handling were 1.76 ± ± 0.93, 3.06 ± 1.25 and 2.44 ± 1.21, respectively. Mean score for neuroticism, fear caused by other dogs, fear caused by human and separation behavior were 29.69 ± 9.24, 14.75 ± 5.38, 9.84 ± 4.05 and 6.57 ± 3.05, respectively. Principal component analysis (PCA) revealed fear using neuroticism, fear caused by other dogs and human were 1st fear related factor while separation‑related behavior was the 2nd priority (P = 0.001). Small‑breed dogs had more separation behavior and fear caused by other dogs compared to the large‑breed dogs (P = 0.001). These findings suggested keeping the dogs indoor with limit access to a yard had negative impact on dog's behaviors. Legal restrictions have adverse effect on fear behavior in dogs. Determination of fear‑related factors might prove useful for dog's behavioral intervention in Iran.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10832437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive meta-analysis of Brucella infections in aquatic mammals. 水生哺乳动物布鲁氏菌感染的综合meta分析。

4区农林科学

Veterinaria italiana Pub Date : 2022-12-30 DOI: 10.12834/VetIt.2427.14954.2

Maryam Dadar, Youcef Shahali, Yadolah Fakhri, Jacques Godfroid

{"title":"A comprehensive meta-analysis of Brucella infections in aquatic mammals.","authors":"Maryam Dadar, Youcef Shahali, Yadolah Fakhri, Jacques Godfroid","doi":"10.12834/VetIt.2427.14954.2","DOIUrl":"https://doi.org/10.12834/VetIt.2427.14954.2","url":null,"abstract":"The presence of Brucella infections was documented in a large number of aquatic mammals, affecting wild animals living in oceans, seas, lakes and rivers within both northern and southern hemispheres. Through meta‑regression analysis, this study provides acom prehensive view of the prevalence of Brucella spp. in aquatic mammals, identifying risksu bgroups as well as most common sampling and testing methods. Brucella ceti and Brucella pinnipedialis represent the main marine Brucella spp., with documented enzootic potential, for which information on standardized diagnostic methods for the implementation of efficient screening and monitoring programs is needed. A total of 71 articles investigating the occurrence of brucellosis in aquatic mammals have been reported since 1987. The prevalence of brucellosis in males (30.42%) was significantly higher than females (18.59%). The family of Delphinidae was the most studied among aquatic mammals with a total prevalence of 39.66%. Our meta‑regression analysis showed a strong and significant association between the prevalence of Brucella spp. in mammals and water temperature (C = 0.02, p value = 0.003), while no significant correlation was found with water salinity (C = ‑ 0.09; p value = 0.10). At least 130 species of aquatic mammals have been identified as potential hosts for Brucella spp. There is no systematic veterinary inspection and global or local requirements for the monitoring of brucellosis in aquatic mammals. The association of brucellosis prevalence and water temperature warrants further studies to assess the potential direct and indirect impacts of climate change on brucellosis in aquatic mammals. This study would help to determine the basis of adaptive management strategies in order to control enzootic brucellosis in wild aquatic mammals.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10815384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salmonella enterica diversity and antimicrobial resistance profile in broiler slaughterhouse by-products. 肉鸡屠宰场副产品中肠沙门氏菌多样性及耐药性分析。

4区农林科学

Veterinaria italiana Pub Date : 2022-12-30 DOI: 10.12834/VetIt.2434.14968.1

Juliana Bonifácio Alcântara, Poliana Carneiro Martins, Eduardo de Paula Nascente, Marcos B Café, Lívia Mendonça Pascoal, Amanda Vargas Teles, Valéria de Sá Jayme, Maria Auxiliadora Andrade

{"title":"Salmonella enterica diversity and antimicrobial resistance profile in broiler slaughterhouse by-products.","authors":"Juliana Bonifácio Alcântara, Poliana Carneiro Martins, Eduardo de Paula Nascente, Marcos B Café, Lívia Mendonça Pascoal, Amanda Vargas Teles, Valéria de Sá Jayme, Maria Auxiliadora Andrade","doi":"10.12834/VetIt.2434.14968.1","DOIUrl":"https://doi.org/10.12834/VetIt.2434.14968.1","url":null,"abstract":"The aim of this study was to investigate the presence of Salmonella enterica in by‑products (feathers, spleen, cecum, and crop) from broiler slaughterhouses as well as to determine the antimicrobial resistance profile of the identified serovars. Forty‑four lots of broilers in nine slaughterhouses located in the central‑west region of Brazil were evaluated. Samples of spleen, feathers, cecum, and crop were collected in a pool and a total of 1,232 samples were evalueted. These were processed for conventional bacterial isolation and subjected to biochemical and serological tests to identify serovars. The identified serovars were subjected to the antimicrobial susceptibility test, where nine different antimycotics were investigated. Salmonella enterica was identified in 7.1% (87/1,232) of all evaluated samples, mostly in feathers (12.3%) and spleen (8.1%). The most frequent serovars were Schwarzengrund (29.9%), Agona (25.4%), Mbandaka (12.7%) and Anatum (8.1%). Nine serovars showed resistance to at least one antimicrobial, especially serovars Mbandaka, Infantis and Typhimurium. Amoxicillin and tetracycline were not effective in inhibiting at least five and four serovars, respectively.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10691833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Status of Urinary shedders of Leptospires among cattle in Tamilnadu: Implications on zoonosis. 泰米尔纳德邦牛尿中钩端螺旋体脱落状况:对人畜共患病的影响。

4区农林科学

Veterinaria italiana Pub Date : 2022-12-30 DOI: 10.12834/VetIt.2478.16135.1

K Senthilkumar, G Ravikumar, Aravindh Babu R Parthiban

{"title":"Status of Urinary shedders of Leptospires among cattle in Tamilnadu: Implications on zoonosis.","authors":"K Senthilkumar, G Ravikumar, Aravindh Babu R Parthiban","doi":"10.12834/VetIt.2478.16135.1","DOIUrl":"https://doi.org/10.12834/VetIt.2478.16135.1","url":null,"abstract":"Bovine leptospirosis causes jaundice, mastitis, infertility, abortion, and death of the animal. This research aimed to study the status of urinary shedders of pathogenic Leptospira among the cattle population and identify the infecting serogroup circulating in this region. A total of 305 blood and 305 urine samples were collected from organized farms (n = 44), individually housed animals (n = 81) and animals from the slaughterhouse (n = 180). Microscopic agglutination test was carried out to detect anti‑leptospiral antibodies. Dark‑field microscopic examination and culture of urine were done to detect and isolate the Leptospira. The isolated Leptospira were identified by cross‑agglutination test and gene sequencing. PCR and real‑time PCR were carried out to detect leptospiral genomic DNA in urine samples to detect the shedders. The anti‑leptospiral antibodies were detected in 6.2% of animals. The Leptospira genomic DNA was detected in 9.2% (28 of 305) of urine samples. Of the 28 Leptospira positive urine samples, 39.2% were from animals with clinical signs suggestive of leptospirosis and 60.8% Leptospira positive samples were from slaughterhouse animals. The Leptospira isolated were identified as Leptospira interrogans serogroup Sejroe and Hebdomadis. The present study demonstrates the need to include leptospirosis in cattle health surveillance programmes to prevent leptospirosis by vaccination, preventing renal carriage.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10637654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved visual closed tube Loop mediated isothermal amplification (LAMP) assay for rapid identification of orf virus in sheep and goats. 改进的肉眼闭管环介导等温扩增(LAMP)法快速鉴定绵羊和山羊口蹄疫病毒。

4区农林科学

Veterinaria italiana Pub Date : 2022-12-30 DOI: 10.12834/VetIt.2426.15340.2

Gnanavel Venkatesan, Anand Kushwaha, Amit Kumar, D P Bora, P Sasikumar

{"title":"An improved visual closed tube Loop mediated isothermal amplification (LAMP) assay for rapid identification of orf virus in sheep and goats.","authors":"Gnanavel Venkatesan, Anand Kushwaha, Amit Kumar, D P Bora, P Sasikumar","doi":"10.12834/VetIt.2426.15340.2","DOIUrl":"https://doi.org/10.12834/VetIt.2426.15340.2","url":null,"abstract":"The orf virus (ORFV) is an epitheliotropic virus causing a highly contagious skin disease mainly in sheep and goats. Several diagnostics including molecular tools like Loop mediated isothermal amplification (LAMP) assay are available to detect ORFV in affected species. However, the carry-over contamination associated with LAMP as open tube format prevents the assay applicability as point of care test in field diagnostic settings. In this study, the B2L gene based LAMP assay was optimized in a closed tube format using hydroxynaphthol blue (HNB) and calcein as pre-addition dyes and it has shown a clear positive and negative signal at 60 °C using 4 and 5 mM concentrations of MgSO4 respectively for these dyes. Optimitimzed assay that could reveal the result within one hour is highly specific and senstive with a limit of detection at 12.5 femtogram of viral genomic DNA or ~85 virus genome equivalent. This improved method prevented the cross-contamination of future LAMP reactions in the laboratory without compromising diagnostic sensitivity (100%) and specificity (100%) when compared to open tube system. This closed tube LAMP method has potential to act as a simple visual detection assay for the rapid and specific diagnosis of ORFV in sheep and goats.","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10815385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0