Production and Characterization of Egg-Derived Antibodies (IgY) Against Outer Membrane Proteins of Multidrug-Resistant Salmonella enterica Serovar Typhimurium.
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引用次数: 0
Abstract
Non-typhoidal salmonellosis is a globally prevalent zoonotic disease, often associated with the growing concern of antimicrobial resistance (AMR). This persistent issue underscores the urgent need for novel strategies for prevention and control. Although conventional approaches such as drug or vaccine development are widely explored, they are often time-consuming, costly, and subject to potential bacterial resistance over time. As an alternative, egg-derived antibodies (IgY) offer a promising solution. IgY can be produced in large quantities without the need for complex infrastructure or environmental risk. Their production is characterized by a favorable safety profile, scalability, high antibody yield per egg yolk, cost-effectiveness, and lack of cross-reactivity in mammals-attributes that collectively make this technology highly advantageous. The present investigation aimed to establish a standardized protocol for hyperimmunization, production, and isolation of IgY antibodies from the yolk of immunized hens targeting multidrug-resistant (MDR) Salmonella enterica serovar Typhimurium. The study further focused on the characterization and specificity assessment of the purified IgY. The findings suggest that the resulting IgY holds strong potential as a therapeutic, prophylactic, or diagnostic agent against MDR S. Typhimurium.
期刊介绍:
The journal was created as the Croce Azzurra in 1950.
A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy.
The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.