Production and Characterization of Egg-Derived Antibodies (IgY) Against Outer Membrane Proteins of Multidrug-Resistant Salmonella enterica Serovar Typhimurium.

IF 0.5 4区 农林科学 Q4 VETERINARY SCIENCES
Aakanksha Tiwari, Rajesh Kumar, Garima Pandey, Namita Shukla, Anupama Mishra, Anuj Tewari
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引用次数: 0

Abstract

Non-typhoidal salmonellosis is a globally prevalent zoonotic disease, often associated with the growing concern of antimicrobial resistance (AMR). This persistent issue underscores the urgent need for novel strategies for prevention and control. Although conventional approaches such as drug or vaccine development are widely explored, they are often time-consuming, costly, and subject to potential bacterial resistance over time. As an alternative, egg-derived antibodies (IgY) offer a promising solution. IgY can be produced in large quantities without the need for complex infrastructure or environmental risk. Their production is characterized by a favorable safety profile, scalability, high antibody yield per egg yolk, cost-effectiveness, and lack of cross-reactivity in mammals-attributes that collectively make this technology highly advantageous. The present investigation aimed to establish a standardized protocol for hyperimmunization, production, and isolation of IgY antibodies from the yolk of immunized hens targeting multidrug-resistant (MDR) Salmonella enterica serovar Typhimurium. The study further focused on the characterization and specificity assessment of the purified IgY. The findings suggest that the resulting IgY holds strong potential as a therapeutic, prophylactic, or diagnostic agent against MDR S. Typhimurium.

抗多药耐药肠炎沙门氏菌血清型鼠伤寒沙门氏菌外膜蛋白的蛋源抗体(IgY)的制备与鉴定
非伤寒沙门氏菌病是一种全球流行的人畜共患疾病,通常与日益受到关注的抗微生物药物耐药性(AMR)有关。这一持续存在的问题突出表明迫切需要制定新的预防和控制战略。尽管药物或疫苗开发等传统方法得到了广泛探索,但它们往往耗时、昂贵,而且随着时间的推移可能会产生细菌耐药性。作为替代方案,蛋源抗体(IgY)提供了一个有希望的解决方案。IgY可以在不需要复杂基础设施或环境风险的情况下大量生产。它们的生产具有良好的安全性、可扩展性、每个蛋黄的高抗体产量、成本效益以及在哺乳动物中缺乏交叉反应性等特点,这些都使该技术具有很高的优势。本研究旨在建立针对多药耐药(MDR)肠沙门氏菌血清型鼠伤寒沙门氏菌的免疫母鸡卵黄中IgY抗体的过度免疫、生产和分离的标准化方案。本研究进一步关注纯化IgY的特性和特异性评估。研究结果表明,由此产生的IgY具有很强的潜力,可作为耐多药鼠伤寒沙门氏菌的治疗、预防或诊断剂。
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来源期刊
Veterinaria italiana
Veterinaria italiana VETERINARY SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
审稿时长
>12 weeks
期刊介绍: The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.
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