Comparative effectiveness of different biological specimens for PRRSv detection in naturally infected fattening pigs.

IF 0.5 4区 农林科学 Q4 VETERINARY SCIENCES
Milena Monnier, Silvia Marro, Andrea Trossi, Marco Faccenda, Nicoletta Vitale, Roberto Zoccola, Ilaria Miceli, Francesca Rossi, Simone Peletto, Alessandro Dondo, Mariella Goria
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引用次数: 0

Abstract

One of the most critical aspects of PRRS outbreak control in swine herds is the reliable virus detection in both newly introduced animals and replacement gilts. In present study we compared the effectiveness of different biological specimens which - alone or in combination - allow to detect PRRSv carrier animals by mean of Reverse Transcriptase nested PCR (RT-nPCR). Five different matrices (serum, nasal swab, oral fluid, tracheobronchial swab and bronchoalveolar lavage fluid - BALF), known to be effective for PRRSv detection, were included in diagnostic efficacy comparison. Thirty-five pigs newly introduced into a fattening unit of a PRRS chronically affected farm were randomly selected to be submitted to serial sampling of each of the matrices above described, during the first three months of fattening period. A Bayesian approach was used to analyze the RT-nPCR results (i.e., positive or negative) of each sampling method and their respective sensitivity was estimated. PRRSv was detected by RT-nPCR in at least one matrix from 58% to 100% of the pigs. Tracheobronchial swabbing, as well as the combination of tracheobronchial swabbing plus bronchoalveolar washing, or tracheobronchial swabbing plus serum sampling were proved to be the most sensitive sampling methods to detect PRRSv in naturally infected live pigs. This study enlightens as the tracheobronchial swabbing associated with RT-nPCR could be the most recommended diagnostic tool for assessing infection dynamics in pig herds.

不同生物标本对自然感染育肥猪PRRSv检测效果的比较。
猪群中PRRS暴发控制的最关键方面之一是在新引入的动物和替代后备母猪中进行可靠的病毒检测。本研究比较了不同生物标本单独或联合应用RT-nPCR检测PRRSv携带动物的有效性。采用血清、鼻拭子、口腔液、气管支气管拭子和支气管肺泡灌洗液(BALF)五种已知可有效检测PRRSv的基质进行诊断疗效比较。随机选择35头新引入PRRS慢性感染农场育肥单元的猪,在育肥期的前三个月进行上述每种基质的连续抽样。采用贝叶斯方法分析每种采样方法的RT-nPCR结果(即阳性或阴性),并估计其各自的灵敏度。RT-nPCR在58% ~ 100%的猪中至少一种基质中检测到PRRSv。气管支气管拭子法以及气管支气管拭子加支气管肺泡冲洗法或气管支气管拭子加血清取样法是检测自然感染活猪PRRSv最敏感的取样方法。该研究提示,与RT-nPCR相关的气管支气管拭子可能是评估猪群感染动态的最推荐的诊断工具。
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来源期刊
Veterinaria italiana
Veterinaria italiana VETERINARY SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
审稿时长
>12 weeks
期刊介绍: The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.
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