从阿尔及利亚羊和牛样本采集的沙门氏菌的基因分型和抗生素敏感性。

IF 0.5 4区 农林科学 Q4 VETERINARY SCIENCES
Siham Nouichi, Lynda Mezali, Taha Mossadak Hamdi, Christian Vinicio Vinueza-Burgos, Lieven De Zutter
{"title":"从阿尔及利亚羊和牛样本采集的沙门氏菌的基因分型和抗生素敏感性。","authors":"Siham Nouichi, Lynda Mezali, Taha Mossadak Hamdi, Christian Vinicio Vinueza-Burgos, Lieven De Zutter","doi":"10.12834/VetIt.3609.32058.2","DOIUrl":null,"url":null,"abstract":"<p><p>The present work investigates the genetic relatedness and antibiotic susceptibility of Salmonella strains collected from the red meat supply chain, highlighting the public health significance of these pathogens. Pulsed-field gel electrophoresis (PFGE-XbaI) was applied to genotype a collection of 84 Salmonella strains isolated from slaughterhouses. The antibiotic susceptibility of these strains to fourteen antimicrobial agents was determined using the minimum inhibitory concentration (MIC) method. The isolates were classified into 22 fingerprints, with two strains being non-typable. The predominant PFGE types identified were Mu1 (n=18), I2 (n=10), and K2 (n=8), indicating a high level of genetic similarity among isolates (>80%). All Salmonella strains exhibited resistance to at least two antimicrobials, with approximately 34.5% displaying resistance to three or more classes of antibiotics. Twelve distinct resistant patterns were identified, and notably, only one colistin-resistant Salmonella strain was detected. These findings underscore the need for ongoing surveillance and control measures in the red meat industry.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"61 2","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genotyping and antibiotic susceptibility of Salmonella strains collected from sheep and cattle samples in Algeria.\",\"authors\":\"Siham Nouichi, Lynda Mezali, Taha Mossadak Hamdi, Christian Vinicio Vinueza-Burgos, Lieven De Zutter\",\"doi\":\"10.12834/VetIt.3609.32058.2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The present work investigates the genetic relatedness and antibiotic susceptibility of Salmonella strains collected from the red meat supply chain, highlighting the public health significance of these pathogens. Pulsed-field gel electrophoresis (PFGE-XbaI) was applied to genotype a collection of 84 Salmonella strains isolated from slaughterhouses. The antibiotic susceptibility of these strains to fourteen antimicrobial agents was determined using the minimum inhibitory concentration (MIC) method. The isolates were classified into 22 fingerprints, with two strains being non-typable. The predominant PFGE types identified were Mu1 (n=18), I2 (n=10), and K2 (n=8), indicating a high level of genetic similarity among isolates (>80%). All Salmonella strains exhibited resistance to at least two antimicrobials, with approximately 34.5% displaying resistance to three or more classes of antibiotics. Twelve distinct resistant patterns were identified, and notably, only one colistin-resistant Salmonella strain was detected. These findings underscore the need for ongoing surveillance and control measures in the red meat industry.</p>\",\"PeriodicalId\":23550,\"journal\":{\"name\":\"Veterinaria italiana\",\"volume\":\"61 2\",\"pages\":\"\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2025-04-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinaria italiana\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.12834/VetIt.3609.32058.2\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinaria italiana","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.12834/VetIt.3609.32058.2","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

本研究调查了从红肉供应链中收集的沙门氏菌菌株的遗传相关性和抗生素敏感性,强调了这些病原体的公共卫生意义。采用脉冲场凝胶电泳(PFGE-XbaI)对从屠宰场分离的84株沙门氏菌进行基因分型。采用最小抑菌浓度(MIC)法测定菌株对14种抗菌药物的药敏。分离菌株经指纹图谱鉴定为22株,其中2株不可分型。鉴定出的主要PFGE类型为Mu1 (n=18)、I2 (n=10)和K2 (n=8),表明分离株间遗传相似性较高(约80%)。所有沙门氏菌菌株都对至少两种抗菌素具有耐药性,其中约34.5%的菌株对三种或更多种抗菌素具有耐药性。鉴定出12种不同的耐药模式,值得注意的是,仅检测到一种耐粘菌素沙门氏菌菌株。这些发现强调了对红肉行业进行持续监测和控制措施的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genotyping and antibiotic susceptibility of Salmonella strains collected from sheep and cattle samples in Algeria.

The present work investigates the genetic relatedness and antibiotic susceptibility of Salmonella strains collected from the red meat supply chain, highlighting the public health significance of these pathogens. Pulsed-field gel electrophoresis (PFGE-XbaI) was applied to genotype a collection of 84 Salmonella strains isolated from slaughterhouses. The antibiotic susceptibility of these strains to fourteen antimicrobial agents was determined using the minimum inhibitory concentration (MIC) method. The isolates were classified into 22 fingerprints, with two strains being non-typable. The predominant PFGE types identified were Mu1 (n=18), I2 (n=10), and K2 (n=8), indicating a high level of genetic similarity among isolates (>80%). All Salmonella strains exhibited resistance to at least two antimicrobials, with approximately 34.5% displaying resistance to three or more classes of antibiotics. Twelve distinct resistant patterns were identified, and notably, only one colistin-resistant Salmonella strain was detected. These findings underscore the need for ongoing surveillance and control measures in the red meat industry.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinaria italiana
Veterinaria italiana VETERINARY SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
审稿时长
>12 weeks
期刊介绍: The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信