Genetic characterization of the Prion Protein Gene in the indigenous Nguni cattle breed of the Kingdom of Eswatini.

IF 0.5 4区农林科学 Q4 VETERINARY SCIENCES

Veterinaria italiana Pub Date : 2025-04-14 DOI:10.12834/VetIt.3560.26921.2

Pumla Bhekiwe Manyatsi, Sedef Erkunt Alak, Hüseyin Can, Ahmet Efe Köseoğlu, Mervenur Güvendi, Cemal Ün

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Abstract

Bovine spongiform encephalopathy (BSE) is one of several fatal neurodegenerative diseases caused by misfolded, infectious prion proteins. Polymorphisms in the prion protein gene (PRNP) associated with BSE susceptibility have been reported in various cattle breeds. However, no studies have been conducted to investigate these genetic variations in the Nguni cattle breed, an indigenous breed in the Kingdom of Eswatini, Southern Africa. The present study aimed to genetically characterize the PRNP gene in Nguni cattle. Both the coding region and regulatory elements-specifically the promoter and intron 1 regions-were analyzed. Three synonymous polymorphisms were identified: Q78Q, P113P, and I226I. Additionally, the Nguni breed exhibited a higher frequency of deletion alleles in two known insertion/deletion (indel) polymorphisms: a 23-bp deletion in the promoter region and a 12-bp deletion in intron 1, compared to insertion alleles. These findings suggest that Nguni cattle may be genetically susceptible to BSE. The results underscore the importance of implementing selective breeding strategies aimed at enhancing resistance to BSE within this indigenous cattle population.

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斯瓦蒂尼王国土著恩古尼牛品种朊蛋白基因的遗传特征。

牛海绵状脑病（BSE）是由传染性朊蛋白错误折叠引起的几种致死性神经退行性疾病之一。与疯牛病易感性相关的朊蛋白基因（PRNP）多态性已在各种牛品种中得到报道。然而，还没有对南部非洲斯瓦蒂尼王国本土品种Nguni牛的这些遗传变异进行研究。本研究旨在对恩古尼牛PRNP基因进行遗传表征。分析了编码区和调控元件，特别是启动子和内含子1区域。鉴定出3个同义多态性：Q78Q、P113P和I226I。此外，与插入等位基因相比，Nguni品种在两个已知的插入/缺失（indel）多态性中表现出更高的缺失等位基因频率：启动子区域23 bp的缺失和内含子1 12 bp的缺失。这些发现表明，恩古尼牛可能在遗传上易患疯牛病。结果强调了实施选择性育种策略的重要性，这些策略旨在增强本地牛群对疯牛病的抵抗力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinaria italiana VETERINARY SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.