Veterinarni Medicina最新文献_第3页

Mammary neoplasms in female dogs: Clinical, diagnostic and therapeutic aspects. 母犬的乳腺肿瘤：临床、诊断和治疗方面。

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2024-04-26 eCollection Date: 2024-04-01 DOI: 10.17221/4/2024-VETMED

Janaina Reato Rueda, Camila Dias Porto, Rodrigo Prevedello Franco, Isabela Bazzo da Costa, Lais Melicio Cintra Bueno, Raul Jose Silva Girio, Fabio Fernando Ribeiro Manhoso, Patricia Cincotto Dos Santos Bueno, Claudia Sampaio Fonseca Repetti

引用次数: 0

Antibacterial effect of curcumin on Salmonella Typhimurium: In vitro and food model studies. 姜黄素对鼠伤寒沙门氏菌的抗菌作用：体外和食物模型研究。

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2024-04-26 eCollection Date: 2024-04-01 DOI: 10.17221/114/2023-VETMED

Goknur Terzi Gulel, Sibel Kanat, Esra Kucukgoz

{"title":"Antibacterial effect of curcumin on Salmonella Typhimurium: In vitro and food model studies.","authors":"Goknur Terzi Gulel, Sibel Kanat, Esra Kucukgoz","doi":"10.17221/114/2023-VETMED","DOIUrl":"10.17221/114/2023-VETMED","url":null,"abstract":"Salmonellosis is a major foodborne disease transmitted from contaminated poultry products worldwide. Although a wide variety of chemical agents are used in the prevention of foodborne Salmonella spp. infections, consumers prefer natural additives, that do not harm human health and do not impair the characteristics of food. Curcumin is a yellow-coloured, hydrophobic polyphenol obtained from the rhizome of the Curcuma longa L. plant known as turmeric. The purpose of this study was to evaluate curcumin's antibacterial activity against S. Typhimurium in chicken meat and in vitro. In the first step, chicken samples were experimentally contaminated with S. Typhimurium at a level of 2.8 × 10-7 CFU/ml. Then, they were kept in a 1, 2, and 3% curcumin solution for 15 minutes. At the end of the treatment, chicken samples were stored at +4 °C. The number of S. Typhimurium in chicken samples was determined according to EN ISO 6579-1. In the result of the study, the number of S. Typhimurium decreased by 2.37, 2.71, and 2.84 log levels at the end of the 6th day as a result of the 1, 2 and 3% curcumin treatment, respectively. The MIC value of curcumin was determined to be 362 μg/ml for S. Typhimurium.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11093645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of vitamin D₃ and a stinging nettle extract on the gastric tissue of rats administered with trinitrobenzene sulfonic acid. 维生素 D3 和荨麻提取物对服用三硝基苯磺酸的大鼠胃组织的影响

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2024-03-28 eCollection Date: 2024-03-01 DOI: 10.17221/111/2023-VETMED

Arzu Gezer, Sukran Yediel Aras, Nurcan Kilic Baygutalp, Ebru Karadag Sari, Gursel Bedir, Behzad Mokhtare, Kadriye Yilmaz

{"title":"Effect of vitamin D3 and a stinging nettle extract on the gastric tissue of rats administered with trinitrobenzene sulfonic acid.","authors":"Arzu Gezer, Sukran Yediel Aras, Nurcan Kilic Baygutalp, Ebru Karadag Sari, Gursel Bedir, Behzad Mokhtare, Kadriye Yilmaz","doi":"10.17221/111/2023-VETMED","DOIUrl":"https://doi.org/10.17221/111/2023-VETMED","url":null,"abstract":"In this study, the effects of vitamin D3 (Vit. D) and a stinging nettle [Urtica dioica L. (UD)] extract were examined using histopathological and immunohistochemical methods in the stomach tissues of an experimentally created rat model of Crohn's disease (CD). The CD model was created using trinitrobenzene sulfonic acid (TNBS). The animals in the study were divided into control, TNBS, TNBS+Vit. D, and TNBS+UD groups. At the end of the experiment, the animals were euthanised and their stomach tissues were evaluated for necrosis, degeneration, apoptosis, and inflammation. Additionally, an immunohistochemical method was applied to determine the somatostatin (SSTR), aquaporin-1 (AQP-1), caspase-3, and tumour necrosis factor-alpha (TNF-α) immunoreactivity in the gastric tissues. In the evaluations, degenerative and necrotic changes and mononuclear cell infiltration areas were observed in the TNBS group, but such changes could be improved with Vit. D and UD applications. The results suggest that the combination of the Vit. D and UD extract may have a protective and therapeutic role in mitigating TNBS-induced damage to the gastric tissues, potentially through the regulation of SSTR, AQP-1, caspase-3, and TNF-α expression. This indicates a promising avenue for further research and the exploration of these compounds in the context of gastrointestinal health.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11016305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province. 东爪哇省奶牛和山羊亚临床乳腺炎牛奶中大肠埃希菌抗生素耐药性基因的鉴定。

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2024-02-28 eCollection Date: 2024-02-01 DOI: 10.17221/80/2023-VETMED

Desy Cahya Widianingrum, Denada Grace Silaban, Wahyu Indra Duwi Fanata, Himmatul Khasanah

{"title":"Identification of antibiotic resistance genes in Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province.","authors":"Desy Cahya Widianingrum, Denada Grace Silaban, Wahyu Indra Duwi Fanata, Himmatul Khasanah","doi":"10.17221/80/2023-VETMED","DOIUrl":"10.17221/80/2023-VETMED","url":null,"abstract":"Antibiotics are still used to treat mastitis in dairy cows in Indonesia. This study aimed to analyse antibiotic resistance genes in Escherichia coli (E. coli) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow's milk and 71 goat's milk samples from both halves of the udder were collected from 67 farms in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical mastitis samples were screened using the California mastitis test (CMT). E. coli was identified by phenotypic and genotypic methods. E. coli was confirmed with a primer specific to the polymerase chain reaction (PCR) technique. Gene resistance of E. coli was tested using the multiplex-PCR (mPCR) technique with primers encoding the genes temoneira enzyme (TEM), oxacillinase (OXA), sulfhydryl variable (SHV), and cefotaximase-munich IV (CTX-M IV). These genes were chosen because mastitis treatment generally uses oxacilline and β-lactam antibiotics. All data obtained were analysed descriptively. The results show that six isolates of E. coli (46.15%) carried a single resistance gene (TEM or SHV) and two isolates (33.33%) were confirmed as multiple drug-resistant organisms (MDROs) (TEM and SHV). The resistance genes were found in samples originating from Blitar, Banyuwangi, Lumajang, and Pasuruan Regencies. This research implies that antibiotic-resistance genes found in E. coli on certain farms are dangerous and may allow gene transmission to other bacteria that make treatment for mastitis or other bacterial infections ineffective.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10966428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concurrent primary splenic lymphoma and mammary gland tumour with polycystic ovaries in a dog. 一只狗同时患有原发性脾淋巴瘤和乳腺肿瘤以及多囊卵巢。

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2024-02-27 eCollection Date: 2024-02-01 DOI: 10.17221/99/2023-VETMED

Seung-Hyun Kim, Yeong-Bin Baek, Sang-Ik Park

引用次数: 0

Evaluating the indicators of a heart rate variability analysis in dogs using Poincaré plots. 利用波恩卡雷图评估狗的心率变异性分析指标。

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2024-02-27 eCollection Date: 2024-02-01 DOI: 10.17221/49/2023-VETMED

Taichi Kimura, Shunsuke Shimamura, Hiroshi Sakaya, Tetsuya Hayashi, Azusa Umemoto, Kosei Sakai, Masahiro Yamasaki, Tetsuya Hasegawa, Terumasa Shimada

{"title":"Evaluating the indicators of a heart rate variability analysis in dogs using Poincaré plots.","authors":"Taichi Kimura, Shunsuke Shimamura, Hiroshi Sakaya, Tetsuya Hayashi, Azusa Umemoto, Kosei Sakai, Masahiro Yamasaki, Tetsuya Hasegawa, Terumasa Shimada","doi":"10.17221/49/2023-VETMED","DOIUrl":"10.17221/49/2023-VETMED","url":null,"abstract":"Heart rate variability analyses using Poincaré plots can be useful for evaluating the autonomic nervous system function. However, the interpretation of the quantitative indicators of Poincaré plots remains controversial. Thus, few studies have verified the effectiveness of the quantitative indicators in veterinary medicine. This study aimed to verify the reliability of Poincaré plot indicators using pharmacological models in dogs. Four healthy beagles were used in this study. Each dog was treated with propranolol, atropine, and propranolol-atropine to block the sympathetic, parasympathetic, and sympathetic-parasympathetic functions, respectively. The quantitative indicators of the Poincaré plots were calculated based on data from 300 electrocardiogram beats collected before and after the administration of each drug and statistically analysed. The quantitative indicators of the Poincaré plots, such as the standard deviation perpendicular to the major axis (SD1), standard deviation along the major axis (SD2), and SD1 × SD2, significantly decreased after the drug administration in both the parasympathetic and sympathetic-parasympathetic blockade models. However, no significant differences were observed in SD1/SD2 between the groups. The Poincaré plots reflected the changes in the autonomic nervous system of dogs. In dogs, SD1, SD2, and SD1 × SD2 can detect a state in which parasympathetic nerve activity is suppressed.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10966429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of praziquantel and its metabolites in grass carp (Ctenopharyngodon idella) following the oral administration of a single bolus. 草鱼（Ctenopharyngodon idella）口服吡喹酮及其代谢物的药代动力学。

IF 0.6 4区农林科学

Veterinarni Medicina Pub Date : 2024-02-27 eCollection Date: 2024-02-01 DOI: 10.17221/109/2023-VETMED

Radka Dobsikova, Jana Blahova, Petr Marsalek, Veronika Doubkova, Eliska Zuskova, Josef Velisek

{"title":"Pharmacokinetics of praziquantel and its metabolites in grass carp (Ctenopharyngodon idella) following the oral administration of a single bolus.","authors":"Radka Dobsikova, Jana Blahova, Petr Marsalek, Veronika Doubkova, Eliska Zuskova, Josef Velisek","doi":"10.17221/109/2023-VETMED","DOIUrl":"10.17221/109/2023-VETMED","url":null,"abstract":"The study aimed to evaluate and compare the routes and rates of the depletion of the antiparasitic praziquantel (PZQ), a derivative of pyrazinoisoquinoline, following its oral administration in grass carp (Ctenopharyngodon idella). We focused on the depletion of PZQ and its major metabolites - cis-hydroxy praziquantel (CPZQ) and trans-hydroxy praziquantel (TPZQ), in water, the plasma, hepatopancreas, kidney, muscle, and skin, following a single oral administration of PZQ in a concentration of 50 mg/kg. Fish were sampled before the drug administration and then eight times in the course of the 30-day-long experiment. Our results indicate the rapid absorption and elimination of PZQ and its metabolites in all the analysed matrices. The most PZQ-burdened tissue was the hepatopancreas, the gill and the skin. In all the samples, the concentration of the drug and its metabolites consistently declined over time. The residue of the parent compound was detected for the longest time in all the tissues. During the study, a significant (P < 0.01) correlation was found within the concentration of PZQ, CPZQ, and TPZQ in the water and all the biological matrices. It was also found that the PZQ residue was not detected below the maximum residue levels (i.e., 20 μg/kg) until 16 days after exposure in the muscle and skin.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10966426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of the T1640C RYR1 mutation indicating malignant hyperthermia in dogs. 检测表明狗患有恶性高热的 T1640C RYR1 突变。

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2023-11-29 eCollection Date: 2023-11-01 DOI: 10.17221/46/2023-VETMED

Jana Haluskova, Beata Holeckova, Lenka Kokulova, Martina Galdikova, Jaroslav Bucan, Viera Schwarzbacherova, Silvia Sedlakova

{"title":"Detection of the T1640C RYR1 mutation indicating malignant hyperthermia in dogs.","authors":"Jana Haluskova, Beata Holeckova, Lenka Kokulova, Martina Galdikova, Jaroslav Bucan, Viera Schwarzbacherova, Silvia Sedlakova","doi":"10.17221/46/2023-VETMED","DOIUrl":"10.17221/46/2023-VETMED","url":null,"abstract":"Malignant hyperthermia (MH) is a clinical syndrome exhibiting elevation of expired carbon dioxide, hyperthermia, muscle rigidity, rhabdomyolysis, acidosis and hyperkalaemia, as well as cardiac dysrhythmia and renal failure. The syndrome manifests itself as a response to anaesthetic agents, such as e.g., halothane, desflurane, and succinylcholine. Depending on the animal species, MH is characterised by autosomal dominant or recessive inheritance, and so far two genes have been identified whose mutations can be linked to MH: RYR1 and CACNA1S. In different species, various mutations of the RYR1 gene have been described which may underlie MH. One of these mutations in dogs is T1640C, which results in the substitution of alanine for valine of the amino acid 547 (V547A) in the RYR1 protein. In our work, we aimed to investigate MH at the DNA level by identifying the T1640C mutation in a group of 50 dogs. For this purpose we used the PCR-RFLP technique, and in six dogs also direct sequencing of PCR products and subsequent comparison of their sequences with the RYR1 gene sequence in an online database. The results of our study show that none of the dogs analysed had any mutant allele of the RYR1 gene, indicating that none should be affected by MH.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10755811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139075074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predominance of Acinetobacter pseudolwoffii among Acinetobacter species in domestic animals in the Czech Republic. 捷克共和国家畜中的假溶血性醋酸杆菌占优势。

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2023-11-29 eCollection Date: 2023-11-01 DOI: 10.17221/65/2023-VETMED

Vladimir Sladecek, David Senk, Petr Stolar, Jaroslav Bzdil, Ondrej Holy

{"title":"Predominance of Acinetobacter pseudolwoffii among Acinetobacter species in domestic animals in the Czech Republic.","authors":"Vladimir Sladecek, David Senk, Petr Stolar, Jaroslav Bzdil, Ondrej Holy","doi":"10.17221/65/2023-VETMED","DOIUrl":"10.17221/65/2023-VETMED","url":null,"abstract":"The aim of this study was to map the spectrum of microorganisms belonging to the genus Acinetobacter in domestic animals with a specific focus on the prevalence of Acinetobacter pseudolwoffii. Additionally, the susceptibility of isolates to antimicrobial agents was determined. In the period from January 1, 2014, to August 31, 2015, a total of 9 544 samples originating from gross lesions and pathological processes of animals exhibiting clinical symptoms of the disease were examined across 41 districts in the Czech Republic. The examinations were carried out using culture methods involving meat-peptone blood agar and Endo agar under aerobic conditions at a temperature of 37 ± 1 °C for 18-24 hours. Isolates were confirmed using molecular phenotypic method MALDI-TOF MS with the MBT Compass Library Revision L 2020 covering 3 239 species/entries (9 607 MSP) from Bruker Daltonics company. Out of the 108 isolates (prevalence 1.13%), 14 species of Acinetobacter spp. were identified, with 5 isolates remaining unclassified as species. A. pseudolwoffii was the predominant species isolated in 25 cases (prevalence 0.26%). Antimicrobial susceptibility was determined for 12 antimicrobials by the disc diffusion method, with A. pseudolwoffii isolates exhibiting the lowest susceptibility to ceftazidime (32%) and co-trimoxazole (60%).","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10755813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139072573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Updating of epidemiological and pathological features of Marek's disease in laying hens and broilers. 更新蛋鸡和肉鸡马立克氏病的流行病学和病理学特征。

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2023-11-27 eCollection Date: 2023-11-01 DOI: 10.17221/75/2023-VETMED

Mourad Zeghdoudi, Merdaci Latifa, Madi Seloua, Sadeddine Rima, Tahri Mardja, Aoun Leila

{"title":"Updating of epidemiological and pathological features of Marek's disease in laying hens and broilers.","authors":"Mourad Zeghdoudi, Merdaci Latifa, Madi Seloua, Sadeddine Rima, Tahri Mardja, Aoun Leila","doi":"10.17221/75/2023-VETMED","DOIUrl":"10.17221/75/2023-VETMED","url":null,"abstract":"Marek's disease (MD) is a huge problem for researchers due to the significant losses in bird flocks, but more importantly, the virus's mutagenic potential. The purpose of this study was to describe non-classical gross lesions observed in broilers and laying hens that suggest the disease emergence and re-emergence. The survey was conducted on 10 broiler and 4 laying hen flocks. All of the dead birds were necropsied in order to obtain a comprehensive diagnosis of lesions, analysing both macroscopic and microscopic alterations. Marek's disease occurred in 80% of cases in broilers and 100% of cases in layer hens. The disease struck 26-day-old broilers and hens at 2 weeks of age, causing a total mortality of 6% and 15%, respectively. There were no clinical indications of the classical neurological form of the disease in either rearing type, and tumour lesions were mostly detected in the liver, spleen, and ovarian follicles in layers, and in the proventriculus in broilers. These findings demonstrated that MD is widespread and that its resurgence is primarily manifested in visceral rather than neurological manifestations. Despite MD immunization, biosecurity remains critical.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10755812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139075076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0