Veterinarni Medicina最新文献_第10页

Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens 甲氧苄啶与磺胺甲恶唑复方在肉鸡血清中的药动学特性的实验研究

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-31 DOI: 10.17221/190/2020-VETMED

Kristina Putecova, K. Nedbalcová, I. Bartejsova, M. Zouharova, Katarína Matiašková, E. Jeklová, Michaela Viskova, Petra Zouzelkova, Martin Jeřábek, K. Šťastný

{"title":"Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens","authors":"Kristina Putecova, K. Nedbalcová, I. Bartejsova, M. Zouharova, Katarína Matiašková, E. Jeklová, Michaela Viskova, Petra Zouzelkova, Martin Jeřábek, K. Šťastný","doi":"10.17221/190/2020-VETMED","DOIUrl":"https://doi.org/10.17221/190/2020-VETMED","url":null,"abstract":"A rapid, simple and highly efficient analytical method for the targeted determination of trimethoprim and sulfamethoxazole in serum samples has been developed and used to measure the pharmacokinetic curve of these medicinal substances after administration to chicken broilers. The pharmacokinetics properties of trimethoprim and sulfamethoxazole were investigated in clinically healthy broiler chickens after the single oral administration of the commercial preparation Methoxasol (Eurovet Animal Health, B.V., The Netherlands) at a dose of 0.275 ml/kg b.w. After a single dose drug administration, the chickens were sacrificed by decapitation under general anaesthesia by Isoflurin 1 000 mg/g (Vetpharma AH, Spain) and the blood was collected at precisely defined intervals: 15, 30, 45, 60, 90, 120, 180, 360 and 720 min after the administration. The serum concentrations of amoxicillin were determined using Q Exactive tandem mass spectrometer (Thermo Fisher Scientific, USA) in conjunction with liquid chromatography. The detected pharmacokinetic parameters of trimethoprim after the oral administration were Cmax = 2.1 ± 1.0 μg/ml; Tmax = 1.5 h; t1⁄2 = 0.88 h; kel = 0.009 3 ± 0.001 1 1/h; AUCt = 2.901 ± 1.4 μg.h/ml; AUC∞ = 2.907 ± 1.5 μg.h/ml; Vd = 2.632 l/kg; Cl = 2.7 l/h. The pharmacokinetic parameters of sulfamethoxazole after the oral administration were Cmax = 47.1 ± 15.3 μg/ml; Tmax = 1 h; t1⁄2 = 1.92 h; kel = 0.004 6 ± 0.000 3 1/h; AUCt = 89.676 ± 26.9 μg.h/ml; AUC∞ = 94.612 ± 28.4 μg.h/ml; Vd = 0.584 l/kg; Cl = 0.21 l/h. To the best of our knowledge, this is the first pharmacokinetic study of the combination of sulfamethoxazole and trimethoprim in broiler chickens.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"42 1","pages":"248-256"},"PeriodicalIF":0.7,"publicationDate":"2021-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73407586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A comprehensive study of canine parvoviruses (Carnivore protoparvovirus 1, Carnivore bocaparvovirus 1 and 2) from shelter dogs in Turkey 土耳其收容所犬细小病毒（食肉动物原细小病毒1、食肉动物博卡病毒1和2）的综合研究

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-12 DOI: 10.17221/130/2020-vetmed

H. Isidan, T. Turan

{"title":"A comprehensive study of canine parvoviruses (Carnivore protoparvovirus 1, Carnivore bocaparvovirus 1 and 2) from shelter dogs in Turkey","authors":"H. Isidan, T. Turan","doi":"10.17221/130/2020-vetmed","DOIUrl":"https://doi.org/10.17221/130/2020-vetmed","url":null,"abstract":"A total of 150 rectal swab samples were collected from diarrheic dogs from the Sivas Municipal Animal Shelter, Turkey in April 2018. While 127 faecal samples were gathered from adults, 23 samples were collected from puppies. Canine parvovirus type 2 (Carnivore protoparvovirus 1), Canine minute virus (Carnivore bocaparvovirus 1) and Canine bocavirus (Carnivore bocaparvovirus 2) were examined by PCR with three sets of novel primers. Some of the amplicons were subjected to molecular sequencing and molecular analysis. Three faecal (2.36%) samples were found to be positive for Carnivore protoparvovirus 1, five faecal samples (3.94%) were found to be positive for Carnivore bocaparvovirus 1 and three samples (2.36%) were found to be positive for Carnivore bocaparvovirus 2 among 127 adult dogs. On the other hand, 14/23 faecal samples (60.87%) were found to be positive for Carnivore protoparvovirus 1, 8/23 faecal samples (34.78%) were found to be positive for Carnivore bocaparvovirus 1, and 6/23 faecal samples (26.09%) were found to be positive for Carnivore bocaparvovirus 2 from the puppies. Moreover, we detected two distinct clades of Carnivore bocaparvovirus 1 and 2 according to the molecular analysis. To the best of our knowledge, this is the first report for the direct detection of Carnivore bocaparvovirus 1 and 2 in Turkey.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43918390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Induction of atopic dermatitis by epicutaneous sensitisation with Dermatophagoides farinae in nongenetically predisposed Beagle dogs 在非遗传易感的比格犬中，由表皮致敏引起的皮肤过敏性皮炎

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-06 DOI: 10.17221/58/2020-vetmed

Y. Jeong, T. Yun, H. Kim, Y. Koo, Jh-E. Kang, M.‐P. Yang, BT Kang

{"title":"Induction of atopic dermatitis by epicutaneous sensitisation with Dermatophagoides farinae in nongenetically predisposed Beagle dogs","authors":"Y. Jeong, T. Yun, H. Kim, Y. Koo, Jh-E. Kang, M.‐P. Yang, BT Kang","doi":"10.17221/58/2020-vetmed","DOIUrl":"https://doi.org/10.17221/58/2020-vetmed","url":null,"abstract":"This study was performed to induce atopic dermatitis (AD) using nongenetically predisposed Beagle dogs. Five healthy Beagle dogs were used. Twice weekly for 12 weeks, the dogs were painted on the axillae and groin with a solution of Dermatophagoides farinae (D. farinae). Each dog was thereafter placed in a cage where a house dust mite (HDM) solution was applied on the bottom of the cage. The dog remained in the cage for 3 h daily for 3 consecutive days for the environmental exposure to HDM. Serum samples were collected at 0 week and 6 weeks after sensitisation, and at 0 h and 72 h after the environmental exposure. During the environmental exposure, skin biopsies were obtained at 0 h, 36 h, and 72 hours. After the first environmental exposure, no dog had any marked clinical sign. An additional sensitisation was subsequently administered for 10–13 weeks. Three of the five dogs developed pruritic dermatitis with skin lesions after the second exposure. The histopathology of the lesions revealed severe infiltration of inflammatory cells and dermal oedema. The levels of D. farinae-specific IgE were also elevated. This study demonstrated that AD could be induced by epicutaneous sensitisation with HDM in nongenetically predisposed dogs.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"2 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41301494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Use of micro-grafts in a chronic infected open wound after limb amputation in a cat 微移植物在猫肢体截肢后慢性感染开放性伤口中的应用

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/197/2020-VETMED

Luca Pennasilico, C. Bella, Riccardo Botto, E. Murgia, V. Riccio, A. Piccionello

引用次数: 2

Closed spinal dysraphism in a 6-month-old mixed breed dog 闭合性脊柱畸形 ，一只6个月大的杂交犬

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/68/2020-VETMED

GG Franco, E. Siqueira, Jal Souza, L.C.B. Prado, S. Rahal, MJ Mamprim, B.W. Minto, Cvs Brandao, JS Costa Jr

{"title":"Closed spinal dysraphism in a 6-month-old mixed breed dog","authors":"GG Franco, E. Siqueira, Jal Souza, L.C.B. Prado, S. Rahal, MJ Mamprim, B.W. Minto, Cvs Brandao, JS Costa Jr","doi":"10.17221/68/2020-VETMED","DOIUrl":"https://doi.org/10.17221/68/2020-VETMED","url":null,"abstract":"The term spinal dysraphism defines an incomplete fusion or a bone defect that affects the neural structures of the spinal cord due to a neural tube malformation. A 6-month-old, male, mixed-breed dog, was evaluated for paraparesis, pelvic limb proprioceptive ataxia, faecal and urinary incontinence. A neurological examination indicated an L4-S3 spinal cord segment lesion. A thoracolumbar and lumbosacral spine magnetic resonance imaging was performed and a closed spinal dysraphism, with the presence of a lipomyelomeningocele, was detected. The magnetic resonance imaging showed an entire narrowing passage and a fibrous mass stemming from a wedgeshaped gap in the caudal region of the dorsal lamina of the L4 vertebra, leaving the spinal canal towards the skin surface in the region where the skin stigma was observed. A dorsal laminectomy was performed, the spinal cord was exposed, and the entire fibrous mass was excised. Three months postoperatively, the patient had a complete resolution of the urinary and faecal incontinence showing only a residual mild paraparesis, which remained six months after surgery. The surgical procedure resulted in the satisfactory recovery of the neurological signs.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"153 1","pages":"219-224"},"PeriodicalIF":0.7,"publicationDate":"2021-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85312427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of the external quality characteristics of goose eggs of known breadth and length 已知宽度和长度的鹅蛋的外部质量特征的估计

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/187/2020-vetmed

O. Karabulut

{"title":"Estimation of the external quality characteristics of goose eggs of known breadth and length","authors":"O. Karabulut","doi":"10.17221/187/2020-vetmed","DOIUrl":"https://doi.org/10.17221/187/2020-vetmed","url":null,"abstract":"In this study, we investigated whether the mathematical formulas, which generally reveal the external quality characteristics of living organisms, yield the correct results in goose eggs. For this purpose, three genotypes and 555 eggs were studied that were grouped into Native (n = 356), Chinese (n = 163) and Linda (n = 36), which were raised in Aksaray province. The averages in the Native, Chinese and Linda geese were 5.43, 5.38 and 5.7 cm, respectively, in breadth; were 8.12, 8.03 and 8.67 cm in length, respectively; the shape index was 66.9, 67.2 and 66.7 cm, respectively; the egg volume was 142.8, 138.8 and 172.5 cm3, respectively; the egg surface area was 136.9, 134.3 and 155.4 cm2, respectively; the egg weight was 159.1, 154.5 and 192.3 g, respectively; the specific gravity of the eggs was 1.11, 1.11 and 1.12 g/cm3, respectively; the shell thickness was 0.510, 0.504 and 0.555 mm, respectively; the shell weight was 14.79, 14.32 and 18.26 g, respectively; the shell volume was 70.06, 67.82 and 86.41 cm3, respectively; the shell specific gravity was 2.111 3, 2.111 0 and 2.113 5 g/cm3, respectively; and the shell ratio was 9.29, 9.26 and 9.49 g, respectively. The number of pores is calculated as 14 828, 14 502 and 17 152, respectively. Although the formulas used give compatible results, there is a need for formulas that calculate closer to the truth rather than formulas that calculate the egg weight and egg shell weight.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46380434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Aggressive behaviour of Hodgkin’s-like lymphoma in a domestic ferret 攻击性行为 霍奇金淋巴瘤 家养雪貂

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/115/2020-VETMED

H.S. Kim, H. Kim, H. Hwang, S. Oh, S. Do

引用次数: 0

Evaluation of the glutathione concentration in whole blood of dairy Holstein cows 评估 谷胱甘肽的浓度 全血 荷斯坦奶牛

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/157/2020-VETMED

A. Nečasová, A. Pechová, R. Bodor, M. Masár

{"title":"Evaluation of the glutathione concentration in whole blood of dairy Holstein cows","authors":"A. Nečasová, A. Pechová, R. Bodor, M. Masár","doi":"10.17221/157/2020-VETMED","DOIUrl":"https://doi.org/10.17221/157/2020-VETMED","url":null,"abstract":"The objective of this study was to evaluate the concentrations of the total (T-GSH), oxidised (GSSG) and reduced (GSH) glutathione in the blood of dairy cows, assess the relationships of the phase and number of lactation, milk production, body condition score (BCS) and selected biochemical parameters on its concentrations. We analysed 79 samples of whole blood from dairy Holstein cows. The concentration of glutathione was assessed by the spectrophotometric enzymatic method. The whole dataset shows the mean concentration of T-GSH, GSSG and GSH of 803 ± 22 µmol/l, 23 ± 2.5 µmol/l and 757 ± 27 µmol/l, respectively. The GSH/GSSG ratio was 95 ± 17. The phase of the lactation had a significant impact on the levels of T-GSH and GSH, but the number of lactation and BCS had no effect. Dry cows had higher levels of T-GSH (938 ± 44 µmol/l) than the fresh (713 ± 46 µmol/l) and peak lactation (785 ± 45 µmol/l) cows. The fresh cows had significantly lower concentrations of GSH (618 ± 44 µmol/l) than the peak lactation (719 ± 46 µmol/l) and dry cows (827 ± 43 µmol/l). On the basis of a regression analysis, blood glutathione was affected mainly by the liver function and energy metabolism. Glutathione as the marker of oxidation stress seems to be a promising tool in monitoring the health and welfare of the herd, yet intensive research in this field remains necessary.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49641511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Congenital hydrocephalus in a Maine Coon foetus detected using ultrasound during pregnancy 在怀孕期间使用超声波检测到的缅因浣熊胎儿先天性脑积水

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/204/2020-VETMED

P. Socha, BM Socha

{"title":"Congenital hydrocephalus in a Maine Coon foetus detected using ultrasound during pregnancy","authors":"P. Socha, BM Socha","doi":"10.17221/204/2020-VETMED","DOIUrl":"https://doi.org/10.17221/204/2020-VETMED","url":null,"abstract":": A congenital defect that blocks the cerebrospinal fluid outflow is the most common cause of hydrocephalus in young cats. It usually manifests itself within a few weeks of birth. Diagnostic imaging is essential. Therefore, it was decided to report on, for the first time, a clinical case of congenital hydrocephalus in a Maine Coon foetus detected using ultrasound during pregnancy. A difference between the size of the skull bones in the Maine Coon foetuses was recognised during a routine pregnancy ultrasound. Analysis of the sonogram of the bones and brain structures of the diagnosed foetus revealed that the features were highly suggestive of a hydrocephalus. Five clinically healthy kittens and one with hydrocephalus were delivered without complications. The necropsy of the malformed foetus was consistent with the ultrasonographic findings. Bacteriological and virological tests were performed and all were negative which suggest a congenital background of the malformation. This is the first reported case of an intrauterine ultrasound diagnosis of congenital hydrocephalus in a Maine Coon of multiple gestation. The ultrasound control along with the foetometry seem to be useful tools to recognise certain patholo-gies of pregnancy in cats, including congenital malformations of various origins, which is of significant diagnostic and prognostic importance.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"1 1","pages":"456-460"},"PeriodicalIF":0.7,"publicationDate":"2021-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90195844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Domestic cat’s internal carotid artery in ontogenesis 家猫颈动脉在个体发生中的作用

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-02 DOI: 10.17221/116/2020-VETMED

Hanna Ziemak, H. Frąckowiak, M. Zdun

{"title":"Domestic cat’s internal carotid artery in ontogenesis","authors":"Hanna Ziemak, H. Frąckowiak, M. Zdun","doi":"10.17221/116/2020-VETMED","DOIUrl":"https://doi.org/10.17221/116/2020-VETMED","url":null,"abstract":"The aim of the study was to trace the presence of the internal carotid artery in the system of cerebral arteries of the domestic cat and to determine the role of this artery in supplying blood to the brain in ontogenesis. The available publications provide ambiguous or even contradictory information. The authors of some studies claim that there is no extracranial segment in the domestic cat’s internal carotid artery. Other authors reported the internal carotid artery in the arterial pattern of the encephalon base. The study was conducted on sixty-one domestic cats: fifteen foetuses, sixteen juvenile cats, and thirty adult cats were analysed. The internal carotid artery – a vessel with a relatively large lumen – was fully preserved in all the foetuses and most of the juvenile animals. This artery was not complete with regard to the adults and some juvenile individuals, because it had lost the extracranial segment as a result of the obliteration process. A precise description of this area is not only of biological, but also of clinical, significance. The knowledge of the anatomical structure of cerebral vessels is particularly important to correctly interpret images obtained during diagnostic tests and to conduct surgical procedures correctly.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"6 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88752784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5