Veterinarni Medicina最新文献_第10页

Optimisation of phagocytosis assay in rainbow trout (Oncorhynchus mykiss) 虹鳟吞噬实验的优化

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-06-01 DOI: 10.17221/189/2020-VETMED

H. Minářová, P. Ondračkova, M. Palíková, J. Mareš, J. Blahova, K. Jarova, M. Faldyna

引用次数: 0

Percutaneous endoscopic limited-lumbosacral-dorsal laminectomy in eight dogs – A cadaveric study 经皮内镜有限腰骶背椎板切除术 8只狗-尸体研究

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-06-01 DOI: 10.17221/33/2020-VETMED

S. Yang, H. Moon, Yong-Hyun Hwang, Hee-Chun Lee, Dongbin Lee, Jae-Hoon Lee

{"title":"Percutaneous endoscopic limited-lumbosacral-dorsal laminectomy in eight dogs – A cadaveric study","authors":"S. Yang, H. Moon, Yong-Hyun Hwang, Hee-Chun Lee, Dongbin Lee, Jae-Hoon Lee","doi":"10.17221/33/2020-VETMED","DOIUrl":"https://doi.org/10.17221/33/2020-VETMED","url":null,"abstract":"This study aimed to investigate the technical feasibility of a percutaneous endoscopic limited-dorsal laminectomy (PELDL) and to evaluate if the decompression and examination of the lumbosacral vertebral canals could be achieved using an endoscope in small dogs. A total of eight fresh canine cadavers were used for the study. Following the injection of a barium and agarose mixture (BA-gel), which simulates intervertebral disc herniation, a PELDL was performed over L7–S1 in these animals. Computed tomography (CT) scans were obtained preand postoperatively to evaluate the surgical outcomes. All the procedures were completed with a clear visualisation of the spinal cord and removal of the BA-gel. The mean surgery time for the PELDL was 30.00 ± 12.01 minutes. In two dogs, iatrogenic nerve root injuries were caused by the surgical instruments during the operation. The CT scans showed that the amount of BA-gel removed was sufficient for a spinal-cord decompression. A PELDL could be used for the BA-gel removal to decompress the spinal cord and provide a clear view of the spinal canal. Therefore, it could be used as an alternative surgical option to treat lumbosacral disc disease in dogs.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"68 1","pages":"279-286"},"PeriodicalIF":0.7,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89080197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital gallbladder agenesis in a 9-month-old Bull Terrier 先天性胆囊发育不全 一只9个月大的牛头梗

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-06-01 DOI: 10.17221/135/2020-VETMED

O. Gójska-Zygner, M. Galanty, B. Degorska, J. Frymus, W. Zygner

{"title":"Congenital gallbladder agenesis in a 9-month-old Bull Terrier","authors":"O. Gójska-Zygner, M. Galanty, B. Degorska, J. Frymus, W. Zygner","doi":"10.17221/135/2020-VETMED","DOIUrl":"https://doi.org/10.17221/135/2020-VETMED","url":null,"abstract":"Congenital gallbladder agenesis is an extremely rare disorder, which has, to the best of our knowledge, only been reported in seventeen dogs (mainly in Japan). In almost all of these cases, gallbladder agenesis or hypoplasia was detected in small dogs. In this report, we present a case of gallbladder agenesis in a 9-month-old intact female Bull Terrier. The clinical signs included diarrhoea, sporadic vomiting, apathy and decreased appetite. The serum biochemistry revealed an increased liver enzyme activity, an increased concentration of serum bile acids and mild hyperbilirubinaemia. A diagnostic laparotomy demonstrated the lack of a gallbladder and dilation of the common bile duct, which was misinterpreted as the gallbladder in the ultrasonographic examination. The histological examination of the liver revealed degenerative changes in the hepatocytes with glycogen accumulation and some necrotic hepatocytes. The therapy included a low protein diet, fluids, silymarin and ursodeoxycholic acid. After nine weeks of therapy, the dog was in good condition, the diarrhoea and vomiting ceased, and the liver function parameters, such as the AST and GLDH activities, and the concentration of bile acids had decreased to reference intervals.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"1 1","pages":"305-312"},"PeriodicalIF":0.7,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87730098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of in vitro (fish cell line) and in vivo (fish and crustacean) acute toxicity tests in aquatic toxicology 水生毒理学中体外(鱼细胞系)和体内(鱼和甲壳类)急性毒性试验的比较

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-06-01 DOI: 10.17221/161/2020-VETMED

J. Kolářová, J. Velíšek, Z. Svobodová

{"title":"Comparison of in vitro (fish cell line) and in vivo (fish and crustacean) acute toxicity tests in aquatic toxicology","authors":"J. Kolářová, J. Velíšek, Z. Svobodová","doi":"10.17221/161/2020-VETMED","DOIUrl":"https://doi.org/10.17221/161/2020-VETMED","url":null,"abstract":"The use of in vitro (fish cell lines) is a cost-effective, very rapid, and informative tool for toxicological assessments. Using the neutral red (NR) assay, we compared the in vitro acute toxicity (20hEC50) of twenty-six chemical substances on a rainbow trout gonad cell line (RTG-2) with their in vivo acute toxicity to Barbados Millions Poecilia reticulata (48hLC50, OECD 203) and crustacean Daphnia magna (48hEC50, OECD 202). The 20hEC50 values obtained by the NR assay were higher in nearly all the cases when compared to the 48hLC50 in P. reticulata and the 48hEC50 in D. magna, indicating that the sensitivity of the RTG-2 cell line was lower compared to P. reticulata and D. magna. A high (r = 0.89) and significant (P < 0.001) correlation was recorded between the 20hEC50 values of the RTG-2 and the 48hEC50 values of D. magna. The correlation between the 20hEC50 values of the RTG-2 and the 48hLC50 values of P. reticulata was lower (r = 0.65; P < 0.001), but also significant. The authors recommend use of the NR assay on the RTG-2 cell lines as a screening protocol to evaluate the toxicity of xenobiotics in aquatic environments to narrow the spectrum of the concentrations for the fish toxicity test.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43333275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens 甲氧苄啶与磺胺甲恶唑复方在肉鸡血清中的药动学特性的实验研究

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-31 DOI: 10.17221/190/2020-VETMED

Kristina Putecova, K. Nedbalcová, I. Bartejsova, M. Zouharova, Katarína Matiašková, E. Jeklová, Michaela Viskova, Petra Zouzelkova, Martin Jeřábek, K. Šťastný

{"title":"Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens","authors":"Kristina Putecova, K. Nedbalcová, I. Bartejsova, M. Zouharova, Katarína Matiašková, E. Jeklová, Michaela Viskova, Petra Zouzelkova, Martin Jeřábek, K. Šťastný","doi":"10.17221/190/2020-VETMED","DOIUrl":"https://doi.org/10.17221/190/2020-VETMED","url":null,"abstract":"A rapid, simple and highly efficient analytical method for the targeted determination of trimethoprim and sulfamethoxazole in serum samples has been developed and used to measure the pharmacokinetic curve of these medicinal substances after administration to chicken broilers. The pharmacokinetics properties of trimethoprim and sulfamethoxazole were investigated in clinically healthy broiler chickens after the single oral administration of the commercial preparation Methoxasol (Eurovet Animal Health, B.V., The Netherlands) at a dose of 0.275 ml/kg b.w. After a single dose drug administration, the chickens were sacrificed by decapitation under general anaesthesia by Isoflurin 1 000 mg/g (Vetpharma AH, Spain) and the blood was collected at precisely defined intervals: 15, 30, 45, 60, 90, 120, 180, 360 and 720 min after the administration. The serum concentrations of amoxicillin were determined using Q Exactive tandem mass spectrometer (Thermo Fisher Scientific, USA) in conjunction with liquid chromatography. The detected pharmacokinetic parameters of trimethoprim after the oral administration were Cmax = 2.1 ± 1.0 μg/ml; Tmax = 1.5 h; t1⁄2 = 0.88 h; kel = 0.009 3 ± 0.001 1 1/h; AUCt = 2.901 ± 1.4 μg.h/ml; AUC∞ = 2.907 ± 1.5 μg.h/ml; Vd = 2.632 l/kg; Cl = 2.7 l/h. The pharmacokinetic parameters of sulfamethoxazole after the oral administration were Cmax = 47.1 ± 15.3 μg/ml; Tmax = 1 h; t1⁄2 = 1.92 h; kel = 0.004 6 ± 0.000 3 1/h; AUCt = 89.676 ± 26.9 μg.h/ml; AUC∞ = 94.612 ± 28.4 μg.h/ml; Vd = 0.584 l/kg; Cl = 0.21 l/h. To the best of our knowledge, this is the first pharmacokinetic study of the combination of sulfamethoxazole and trimethoprim in broiler chickens.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"42 1","pages":"248-256"},"PeriodicalIF":0.7,"publicationDate":"2021-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73407586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A comprehensive study of canine parvoviruses (Carnivore protoparvovirus 1, Carnivore bocaparvovirus 1 and 2) from shelter dogs in Turkey 土耳其收容所犬细小病毒（食肉动物原细小病毒1、食肉动物博卡病毒1和2）的综合研究

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-12 DOI: 10.17221/130/2020-vetmed

H. Isidan, T. Turan

{"title":"A comprehensive study of canine parvoviruses (Carnivore protoparvovirus 1, Carnivore bocaparvovirus 1 and 2) from shelter dogs in Turkey","authors":"H. Isidan, T. Turan","doi":"10.17221/130/2020-vetmed","DOIUrl":"https://doi.org/10.17221/130/2020-vetmed","url":null,"abstract":"A total of 150 rectal swab samples were collected from diarrheic dogs from the Sivas Municipal Animal Shelter, Turkey in April 2018. While 127 faecal samples were gathered from adults, 23 samples were collected from puppies. Canine parvovirus type 2 (Carnivore protoparvovirus 1), Canine minute virus (Carnivore bocaparvovirus 1) and Canine bocavirus (Carnivore bocaparvovirus 2) were examined by PCR with three sets of novel primers. Some of the amplicons were subjected to molecular sequencing and molecular analysis. Three faecal (2.36%) samples were found to be positive for Carnivore protoparvovirus 1, five faecal samples (3.94%) were found to be positive for Carnivore bocaparvovirus 1 and three samples (2.36%) were found to be positive for Carnivore bocaparvovirus 2 among 127 adult dogs. On the other hand, 14/23 faecal samples (60.87%) were found to be positive for Carnivore protoparvovirus 1, 8/23 faecal samples (34.78%) were found to be positive for Carnivore bocaparvovirus 1, and 6/23 faecal samples (26.09%) were found to be positive for Carnivore bocaparvovirus 2 from the puppies. Moreover, we detected two distinct clades of Carnivore bocaparvovirus 1 and 2 according to the molecular analysis. To the best of our knowledge, this is the first report for the direct detection of Carnivore bocaparvovirus 1 and 2 in Turkey.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43918390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Induction of atopic dermatitis by epicutaneous sensitisation with Dermatophagoides farinae in nongenetically predisposed Beagle dogs 在非遗传易感的比格犬中，由表皮致敏引起的皮肤过敏性皮炎

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-06 DOI: 10.17221/58/2020-vetmed

Y. Jeong, T. Yun, H. Kim, Y. Koo, Jh-E. Kang, M.‐P. Yang, BT Kang

{"title":"Induction of atopic dermatitis by epicutaneous sensitisation with Dermatophagoides farinae in nongenetically predisposed Beagle dogs","authors":"Y. Jeong, T. Yun, H. Kim, Y. Koo, Jh-E. Kang, M.‐P. Yang, BT Kang","doi":"10.17221/58/2020-vetmed","DOIUrl":"https://doi.org/10.17221/58/2020-vetmed","url":null,"abstract":"This study was performed to induce atopic dermatitis (AD) using nongenetically predisposed Beagle dogs. Five healthy Beagle dogs were used. Twice weekly for 12 weeks, the dogs were painted on the axillae and groin with a solution of Dermatophagoides farinae (D. farinae). Each dog was thereafter placed in a cage where a house dust mite (HDM) solution was applied on the bottom of the cage. The dog remained in the cage for 3 h daily for 3 consecutive days for the environmental exposure to HDM. Serum samples were collected at 0 week and 6 weeks after sensitisation, and at 0 h and 72 h after the environmental exposure. During the environmental exposure, skin biopsies were obtained at 0 h, 36 h, and 72 hours. After the first environmental exposure, no dog had any marked clinical sign. An additional sensitisation was subsequently administered for 10–13 weeks. Three of the five dogs developed pruritic dermatitis with skin lesions after the second exposure. The histopathology of the lesions revealed severe infiltration of inflammatory cells and dermal oedema. The levels of D. farinae-specific IgE were also elevated. This study demonstrated that AD could be induced by epicutaneous sensitisation with HDM in nongenetically predisposed dogs.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"2 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41301494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Use of micro-grafts in a chronic infected open wound after limb amputation in a cat 微移植物在猫肢体截肢后慢性感染开放性伤口中的应用

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/197/2020-VETMED

Luca Pennasilico, C. Bella, Riccardo Botto, E. Murgia, V. Riccio, A. Piccionello

引用次数: 2

Closed spinal dysraphism in a 6-month-old mixed breed dog 闭合性脊柱畸形 ，一只6个月大的杂交犬

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/68/2020-VETMED

GG Franco, E. Siqueira, Jal Souza, L.C.B. Prado, S. Rahal, MJ Mamprim, B.W. Minto, Cvs Brandao, JS Costa Jr

{"title":"Closed spinal dysraphism in a 6-month-old mixed breed dog","authors":"GG Franco, E. Siqueira, Jal Souza, L.C.B. Prado, S. Rahal, MJ Mamprim, B.W. Minto, Cvs Brandao, JS Costa Jr","doi":"10.17221/68/2020-VETMED","DOIUrl":"https://doi.org/10.17221/68/2020-VETMED","url":null,"abstract":"The term spinal dysraphism defines an incomplete fusion or a bone defect that affects the neural structures of the spinal cord due to a neural tube malformation. A 6-month-old, male, mixed-breed dog, was evaluated for paraparesis, pelvic limb proprioceptive ataxia, faecal and urinary incontinence. A neurological examination indicated an L4-S3 spinal cord segment lesion. A thoracolumbar and lumbosacral spine magnetic resonance imaging was performed and a closed spinal dysraphism, with the presence of a lipomyelomeningocele, was detected. The magnetic resonance imaging showed an entire narrowing passage and a fibrous mass stemming from a wedgeshaped gap in the caudal region of the dorsal lamina of the L4 vertebra, leaving the spinal canal towards the skin surface in the region where the skin stigma was observed. A dorsal laminectomy was performed, the spinal cord was exposed, and the entire fibrous mass was excised. Three months postoperatively, the patient had a complete resolution of the urinary and faecal incontinence showing only a residual mild paraparesis, which remained six months after surgery. The surgical procedure resulted in the satisfactory recovery of the neurological signs.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"153 1","pages":"219-224"},"PeriodicalIF":0.7,"publicationDate":"2021-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85312427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of the external quality characteristics of goose eggs of known breadth and length 已知宽度和长度的鹅蛋的外部质量特征的估计

IF 0.7 4区农林科学

Veterinarni Medicina Pub Date : 2021-05-03 DOI: 10.17221/187/2020-vetmed

O. Karabulut

{"title":"Estimation of the external quality characteristics of goose eggs of known breadth and length","authors":"O. Karabulut","doi":"10.17221/187/2020-vetmed","DOIUrl":"https://doi.org/10.17221/187/2020-vetmed","url":null,"abstract":"In this study, we investigated whether the mathematical formulas, which generally reveal the external quality characteristics of living organisms, yield the correct results in goose eggs. For this purpose, three genotypes and 555 eggs were studied that were grouped into Native (n = 356), Chinese (n = 163) and Linda (n = 36), which were raised in Aksaray province. The averages in the Native, Chinese and Linda geese were 5.43, 5.38 and 5.7 cm, respectively, in breadth; were 8.12, 8.03 and 8.67 cm in length, respectively; the shape index was 66.9, 67.2 and 66.7 cm, respectively; the egg volume was 142.8, 138.8 and 172.5 cm3, respectively; the egg surface area was 136.9, 134.3 and 155.4 cm2, respectively; the egg weight was 159.1, 154.5 and 192.3 g, respectively; the specific gravity of the eggs was 1.11, 1.11 and 1.12 g/cm3, respectively; the shell thickness was 0.510, 0.504 and 0.555 mm, respectively; the shell weight was 14.79, 14.32 and 18.26 g, respectively; the shell volume was 70.06, 67.82 and 86.41 cm3, respectively; the shell specific gravity was 2.111 3, 2.111 0 and 2.113 5 g/cm3, respectively; and the shell ratio was 9.29, 9.26 and 9.49 g, respectively. The number of pores is calculated as 14 828, 14 502 and 17 152, respectively. Although the formulas used give compatible results, there is a need for formulas that calculate closer to the truth rather than formulas that calculate the egg weight and egg shell weight.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46380434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3