Veterinary Pathology最新文献_第4页

Mesangial cell hypercellularity and iron accumulation in the kidney associated with administration of a sickle hemoglobin modulator in CD-1 mice. CD-1小鼠肾系膜细胞高细胞化和铁积累与镰状血红蛋白调节剂的施用有关。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-01 Epub Date: 2024-12-23 DOI: 10.1177/03009858241306400

Shambhunath Choudhary, Catherine Picut, Sarah R Vargas, Diana Otis, Timothy M Coskran, David Karanian, Jamie K DaSilva, Christopher Houle, Laurence O Whiteley

{"title":"Mesangial cell hypercellularity and iron accumulation in the kidney associated with administration of a sickle hemoglobin modulator in CD-1 mice.","authors":"Shambhunath Choudhary, Catherine Picut, Sarah R Vargas, Diana Otis, Timothy M Coskran, David Karanian, Jamie K DaSilva, Christopher Houle, Laurence O Whiteley","doi":"10.1177/03009858241306400","DOIUrl":"10.1177/03009858241306400","url":null,"abstract":"The kidney plays an important role in iron homeostasis and mesangial cells (MCs) are phagocytic cells important for glomerular homeostasis. Sickle hemoglobin (HbS) modulators are promising clinical candidates for treatment of sickle cell disease. Although they prevent disease pathophysiology of HbS polymerization and red blood cell (RBC) sickling by increasing hemoglobin oxygen affinity, higher oxygen affinity can also cause transient tissue hypoxia with compensatory increases in erythropoiesis and subsequent increases in RBC turnover. CD-1 mice treated with an HbS modulator for 2 weeks developed higher RBC mass, increased erythropoiesis, and, by 1 month, deposition of intracellular pigments in renal tubular and parietal epithelium. In addition, in mice treated for 26 weeks, pigment was observed in MCs, which was accompanied by glomerular cell aggregates (MC hypercellularity) and tubulo-interstitial inflammation. The pigment was confirmed by Perl's iron staining and transmission electron microscopy (TEM) to be iron-containing proteins. Glomerular cell aggregates were confirmed to be MCs by TEM, and Ki-67 immunolabeling suggested that MC hypercellularity was due to proliferation. Collectively, these findings, along with iron-containing proteins in livers and spleens, suggested that iron overload secondary to increased RBC turnover led to increased renal iron reabsorption. While both MC hypercellularity and tubulo-interstitial inflammation were thought to be responses to long-term accumulation of iron, the former was considered a homeostatic response to eliminate iron, and maintain glomerular structure and function, while the latter was more consistent with an iron-catalyzed oxidative stress response. To our knowledge, this is the first report of MC hypercellularity in a preclinical toxicity study.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"397-407"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Holding time or fixative formulation has no obvious effect on histology of Porites evermanni and Montipora capitata. 保存时间或固定剂配方对 Porites evermanni 和 Montipora capitata 的组织学没有明显影响。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-01 Epub Date: 2025-01-05 DOI: 10.1177/03009858241309403

Thierry M Work, Chutimon Singhakarn, Amy Webb, Norton Chan, Michelle M Dennis

引用次数: 0

Hepatic iron assessment using pinch liver biopsies in Asian glossy starlings (Aplonis panayensis). 亚洲斑椋鸟（Aplonis panayensis）肝铁的捏取活检评估。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-01 Epub Date: 2024-12-30 DOI: 10.1177/03009858241309395

Alberto Rodriguez Barbon, Charlotte Bentley, Gabby J Drake, Miguel Mendes Veiga, Julian Chantrey, Guido Rocchigiani

{"title":"Hepatic iron assessment using pinch liver biopsies in Asian glossy starlings (Aplonis panayensis).","authors":"Alberto Rodriguez Barbon, Charlotte Bentley, Gabby J Drake, Miguel Mendes Veiga, Julian Chantrey, Guido Rocchigiani","doi":"10.1177/03009858241309395","DOIUrl":"10.1177/03009858241309395","url":null,"abstract":"Hepatic hemosiderosis is a common nutritional disease affecting numerous bird species. The aim of this study was to validate the use of pinch hepatic biopsies to quantify the hepatic iron in Asian glossy starlings (AGS) and compare clinical measures usable for antemortem disease monitoring. Following euthanasia, serum, liver pinch biopsies, and whole liver sections were sampled from 21 AGS. Liver biopsies and sections were stained with Perl's Prussian blue, and the area containing iron pigment was calculated by image analysis. The mean percent iron-stained area per whole hepatic section was 62.52% ± 21.81%, while the mean percent iron-stained area in biopsies was 71.47% ± 19.77%. Positive correlations were found between hepatic iron concentration and percentage iron in whole sections (r = 0.92; P < .0001), percentage iron in biopsies (r = 0.91; P < .0001), and other clinico-pathological features. Hemosiderin in hepatic biopsies was significantly higher than in whole hepatic section assessed by image analysis (P < .0001).","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":"62 3","pages":"360-363"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective analysis of clustered neuroinflammatory and neurodegenerative diseases in captive lions in the early 1970s. 20世纪70年代早期圈养狮子聚集性神经炎症和神经退行性疾病的回顾性分析。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-04-30 DOI: 10.1177/03009858251335280

Madeleine de le Roi, Inga Nägler, Dennis Rubbenstroth, Martin Beer, Dirk Höper, Stefanie A Barth, Adnan Fayyad, Christina Puff, Wolfgang Baumgärtner, Peter Wohlsein

{"title":"Retrospective analysis of clustered neuroinflammatory and neurodegenerative diseases in captive lions in the early 1970s.","authors":"Madeleine de le Roi, Inga Nägler, Dennis Rubbenstroth, Martin Beer, Dirk Höper, Stefanie A Barth, Adnan Fayyad, Christina Puff, Wolfgang Baumgärtner, Peter Wohlsein","doi":"10.1177/03009858251335280","DOIUrl":"https://doi.org/10.1177/03009858251335280","url":null,"abstract":"Rustrela virus (RusV), a recently discovered pathogen for domestic and wildlife animals, was identified as the causative agent of meningoencephalomyelitis in domestic cats and various zoo animals including lions. To analyze a past outbreak of increased mortality in lions and to reveal its possible etiological relationship with an RusV infection, this retrospective study re-evaluates 20 cases of lions originating from a zoo in Western Germany using archived formalin-fixed paraffin-embedded (FFPE) tissues. Animals with different neurologic signs were submitted for necropsy between December 1970 and April 1971. Eight lions (40%) suffered from non-suppurative meningoencephalomyelitis with RusV RNA and antigen detectable in the central nervous system (CNS). Twelve animals (60%) were negative for RusV. Eleven animals had an etiologically undetermined degenerative encephalomyelopathy characterized by dilated myelin sheaths, myelinophages, and spheroids. Eight of these 12 lions suffered from an erosive, lymphohistiocytic enteritis with nuclear inclusion bodies in enterocytes associated with parvoviral antigen and nucleic acid in the intestines, lymph nodes, and spleen, but not in the CNS. Five of the parvovirus-infected animals had a granulomatous inflammation in mesenteric lymph nodes that was also the only detectable lesion in one other lion. Acid-fast bacilli and Mycobacterium bovis DNA confirmed the diagnosis of tuberculosis. In summary, this study provides convincing evidence of the usefulness of long-term stored FFPE material for further investigations using immunohistochemistry, reverse transcription quantitative polymerase chain reaction, and in situ hybridization for resolving past disease outbreaks. It provides further insights into the epidemiology of infectious agents like RusV and parvovirus.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251335280"},"PeriodicalIF":2.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital pathology in veterinary clinical pathology: A review. 数字病理学在兽医临床病理学中的应用综述。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-04-29 DOI: 10.1177/03009858251334340

Julie Piccione, Stephanie F Anderson, Samuel V Neal, Mara S Varvil

{"title":"Digital pathology in veterinary clinical pathology: A review.","authors":"Julie Piccione, Stephanie F Anderson, Samuel V Neal, Mara S Varvil","doi":"10.1177/03009858251334340","DOIUrl":"https://doi.org/10.1177/03009858251334340","url":null,"abstract":"Digital pathology has rapidly evolved in the field of veterinary medicine. Although digital histology advancements are widely discussed, clinical pathology specimens are also being digitized for a variety of purposes. These digital images can be used for diagnostic evaluation, teaching, training, and research. With recent advancements in artificial intelligence technologies, there is also potential for image analysis of clinical pathology specimens. In contrast to histologic specimens, veterinary practices can process and digitize cytologic specimens in-house. This creates workflows and analytical considerations that differ greatly from the digital pathology process in anatomic pathology. Through the evaluation of published literature, conference proceedings, and commercially advertised services or analyzers, this review summarizes available digital diagnostic services, discusses virtual microscopy in education and research, and explains the concepts of image analysis. We also highlight gaps in the literature regarding digitizing clinical pathology specimens. Finally, we review the overall benefits and limitations of digitization, focusing on the unique aspects of veterinary clinical pathology.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251334340"},"PeriodicalIF":2.3,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review of tracheal neoplasia in dogs and cats (1961-2024). 狗和猫的气管肿瘤（1961-2024）的回顾。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-04-23 DOI: 10.1177/03009858251335208

Alexis Berrocal, Tatiane Terumi Negrão Watanabe, Emily J Brinker, Clinson C Lui, Daniel Felipe Barrantes Murillo

{"title":"Review of tracheal neoplasia in dogs and cats (1961-2024).","authors":"Alexis Berrocal, Tatiane Terumi Negrão Watanabe, Emily J Brinker, Clinson C Lui, Daniel Felipe Barrantes Murillo","doi":"10.1177/03009858251335208","DOIUrl":"https://doi.org/10.1177/03009858251335208","url":null,"abstract":"Tracheal neoplasia is considered infrequent in domestic animals. A detailed summarized description of the demographic trends of the patients and frequent tumor types arising from this anatomical location is missing in the reference literature. To better describe clinical and pathological features, a multi-institutional retrospective analysis and literature review were conducted to collect all the tracheal neoplasms reported in dogs and cats. Forty-two cases from a multi-institutional data search and 123 documented cases from the veterinary literature between 1961 and August 2024 were collected for a total of 165 cases. Dogs represented 41.2% (68/165) of the cases retrieved, whereas the remaining 58.8% (97/165) were cats. The most common tracheal neoplasia in dogs in descending order were osteochondroma, plasma cell tumor, chondrosarcoma, malignant epithelial tumors (adenocarcinoma and carcinoma), and chondroma. In dogs, most of the affected animals were males (53%), with a mean age at the time of diagnosis of 6.7 years, and most of the tumors were located at the cervical trachea (43%). Labrador retrievers represented 10% of the cases. In cats, the most common tracheal neoplasms were lymphoma and malignant epithelial tumors (adenocarcinoma, carcinoma, and squamous cell carcinoma). In cats, most of the affected animals were males (52%) and domestic shorthairs (62%), with a mean age at the time of diagnosis of 10.7 years, and most of the tumors were located at the cervical trachea (38%). Diagnosticians must consider these differential diagnoses when dealing with tracheal samples that are suspicious of neoplasia in dogs and cats.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251335208"},"PeriodicalIF":2.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurotropic enteric viruses in animals: Comparative research, knowledge gaps, and the role of pathology. 动物嗜神经肠道病毒：比较研究、知识差距和病理学的作用。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-04-22 DOI: 10.1177/03009858251334347

Kara L D Dawson, Torsten Seuberlich, Patricia A Pesavento

{"title":"Neurotropic enteric viruses in animals: Comparative research, knowledge gaps, and the role of pathology.","authors":"Kara L D Dawson, Torsten Seuberlich, Patricia A Pesavento","doi":"10.1177/03009858251334347","DOIUrl":"https://doi.org/10.1177/03009858251334347","url":null,"abstract":"Enteric viruses are commonly associated with gastrointestinal diseases but have the capacity, with mostly unknown triggers, to invade the central nervous system (CNS). Neuropathogenic enteric viruses (NEV) that are able to cross or bypass the blood-brain barrier cause debilitating neurological inflammation and disease. The most notorious example of an enteric virus with potential neurotropism is poliovirus, a member of the Picornaviridae family. While poliovirus has been largely eradicated due to extensive vaccination programs, other nonpolio picornaviruses, as well as enteric viruses of other families, are increasingly recognized as causative agents in cases of encephalitis of unknown origin. In the past decade, cutting-edge high-throughput sequencing and bioinformatics strategies have revealed an increasing number of NEV associated with neurological diseases in various animal species. Information, especially pathogenesis studies, on animal enteric viruses with neurotropism is relatively scarce. This review provides an overview of known enteric viruses that invade the CNS, which should support our awareness of the potential etiologic agents and encourage a diagnostic plan that includes NEV. The many knowledge gaps in host susceptibility and viral pathogenesis along the gut-brain axis would benefit from increased discovery efforts and a deeper understanding of the pathogenesis and potential of enteric viruses affecting the nervous system of animals. Crossing of species barriers is common among enteric viruses, so a one-health approach to increase awareness of animal and human NEV would contribute to effective strategies to monitor, manage, and contain emerging zoonotic outbreaks.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251334347"},"PeriodicalIF":2.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neoplastic cell palisades and perinecrotic palisades in veterinary neuropathology. 动物神经病理学中的肿瘤细胞栅栏和会阴坏死栅栏。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-04-22 DOI: 10.1177/03009858251334345

Daniel R Rissi, Brian A Summers

引用次数: 0

Immunohistochemical characterization of normal feline adrenal tissue and adrenal tumors secreting aldosterone. 正常猫肾上腺组织和肾上腺肿瘤分泌醛固酮的免疫组织化学特征。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-04-21 DOI: 10.1177/03009858251331146

Alice Watson, Frederik Allan, Norelene Harrington, Orla Mahony, Morris Brown, Harriet Syme

{"title":"Immunohistochemical characterization of normal feline adrenal tissue and adrenal tumors secreting aldosterone.","authors":"Alice Watson, Frederik Allan, Norelene Harrington, Orla Mahony, Morris Brown, Harriet Syme","doi":"10.1177/03009858251331146","DOIUrl":"https://doi.org/10.1177/03009858251331146","url":null,"abstract":"It is suggested that primary hyperaldosteronism (PHA) is an under-diagnosed cause of systemic hypertension in cats. No immunohistochemical markers of aldosterone synthesizing tissues have been identified, meaning that endocrine function cannot be inferred from examining feline tissues. In humans, expression of CYP11B2 (aldosterone synthase) is used for this purpose, but cats have a single CYP11B enzyme responsible for the terminal steps in synthesis of both aldosterone and cortisol, precluding its use as an indicator of steroidogenic function. This study aimed to identify immunohistochemical markers of aldosterone producing tissues. In addition, since there are no existing guidelines for classification of feline adrenal tumors as benign or malignant, this study aimed to investigate potential markers of adrenal tumor malignancy in PHA. Normal adrenals (n = 9) and adrenal tumors secreting aldosterone (n = 31) or cortisol (n = 4) were immunolabeled for steroidogenic enzymes (CYP11B, CYP17A1, and CYB5A), the zona glomerulosa (including KCNJ5 visinin-like 1 (VSNL1), and neuron-specific enolase (NSE)), and proliferation markers (Ki67). Histochemical staining for reticulin was also performed. Transcriptomes of normal (n = 4) and aldosterone secreting tumors (n = 5) were compared. Weak or absent CYP17A1 in conjunction with strong KCNJ5 or VSNL1 immunolabeling was present in aldosterone producing tissues from normal and tumorous cat adrenals. CYP17A1 RNA expression was lower in aldosterone producing tumors compared with normal adrenals (P < .0001). VSNL1 and NSE were not specific markers of aldosterone producing tissue. CYB5A and CYP17A1 were not expressed within the zona reticularis, suggesting minimal adrenal production of androgens. Ki67 proliferative index and reticulin network disruption were not predictive of malignancy.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251331146"},"PeriodicalIF":2.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical characterization of differentiation-associated transcription factors, tumor suppressor genes, and mismatch repair genes in rabbit uterine adenocarcinoma. 兔子宫腺癌中分化相关转录因子、肿瘤抑制基因和错配修复基因的免疫组化特征。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-04-21 DOI: 10.1177/03009858251332005

Yukino Machida, Sayuri Harashima, Viviana Gonzalez Astudillo, Masaki Michishita

{"title":"Immunohistochemical characterization of differentiation-associated transcription factors, tumor suppressor genes, and mismatch repair genes in rabbit uterine adenocarcinoma.","authors":"Yukino Machida, Sayuri Harashima, Viviana Gonzalez Astudillo, Masaki Michishita","doi":"10.1177/03009858251332005","DOIUrl":"https://doi.org/10.1177/03009858251332005","url":null,"abstract":"The development of human uterine cancer is a complex process involving the abnormal expression of tumor suppressors, such as PTEN, ARID1A, and TP53; mismatch repair protein MSH6; and transcription factors, such as PAX2 and PAX8. The functional changes that lead to uterine adenocarcinoma in pet rabbits are not fully understood despite the frequent occurrence of this condition in the species. Thus, an immunohistochemical analysis was performed to visualize the protein expression pattern of carcinogenesis-related molecules in surgical biopsy specimens from 56 uterine adenocarcinomas and 8 uteruses without significant lesions in pet rabbits. Seventy percent of the adenocarcinomas were positive for estrogen receptor (ER), 7% were positive for TP53, and there was a decreased expression in comparison to control uterine epithelium for PAX2 in 54%, for PAX8 in 73%, for ARID1A in 68%, and for MSH6 in 48% of the tumors. TP53 expression was not observed in control uterine tissues. There was a significant negative correlation between nuclear ER and PAX2 immunolabeling in uterine adenocarcinomas. Heat map analysis classified samples into 4 clusters, which revealed that 1 PAX2-positive group had a higher presence of papillary-type uterine adenocarcinomas and a lower prevalence of tubular/solid types compared with the 2 PAX2-negative groups. This study demonstrated that the immunohistochemical phenotype of rabbit uterine adenocarcinoma is comparable to that of human endometrial carcinomas, suggesting the potential for similar oncogenic mechanisms that might prove useful for translational medicine research.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251332005"},"PeriodicalIF":2.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0