Veterinary Pathology最新文献_第4页

Proliferation scores in canine anal sac adenocarcinomas: Ki67 global score is superior to Ki67 hotspot indices and mitotic count for prognosis. 犬肛囊腺癌的增殖评分：Ki67总体评分优于Ki67热点指数和有丝分裂计数。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-20 DOI: 10.1177/03009858251338855

Barbara Bacci, Barbara Brunetti, Giancarlo Avallone, Ambra Morisi, Ginevra Martinoli, Nicholas J Bacon

{"title":"Proliferation scores in canine anal sac adenocarcinomas: Ki67 global score is superior to Ki67 hotspot indices and mitotic count for prognosis.","authors":"Barbara Bacci, Barbara Brunetti, Giancarlo Avallone, Ambra Morisi, Ginevra Martinoli, Nicholas J Bacon","doi":"10.1177/03009858251338855","DOIUrl":"https://doi.org/10.1177/03009858251338855","url":null,"abstract":"Canine anal sac adenocarcinoma (ASAC) is an aggressive malignancy with high metastatic potential. Histologic and proliferation parameters such as mitotic count and Ki67 scores have limited prognostic value according to the published literature. Using pathologist-supervised digital image analysis methods with the image analysis software QuPath, we analyzed 58 cases of ASAC to evaluate mitotic count (MC) and Ki67 indices, explore relationships between different Ki67 indices [semi-automatic Ki67 digital hotspot score (Ki67-saHDS), Ki67 global digital score (Ki67-GDS), and fully automatic Ki67 digital hotspot score (Ki67-faHDS)] and MC, and to verify which method carries the most significant prognostic value. The MC did not impact median tumor-related survival (TRS) time. Although high correlation coefficients were observed between the 3 Ki67 scores, Ki67-GDS had more prognostic relevance than hotspot-based scores (Ki67-saHDS and Ki67-faHDS). Dogs with Ki67-GDS ≥ 26 had significantly shorter survival times (175, days 95%, confidence interval (95% CI) = 123-540) compared to dogs with Ki67-GDS< 26 (median survival time (MST) 650 days, 95% CI = 503->1579). No association was observed between TRS and Ki67-faHDS or Ki67-saHDS. On multivariate analysis, anisokaryosis and Ki67-GDS, but not tumor size, lymphovascular invasion, or MC, were independent prognostic markers for survival. These results demonstrate the advantage of Ki67 GDS over hotspot-based scores; however, these data need to be validated in a larger cohort of cases before clinical implementation.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251338855"},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feline Hodgkin-like lymphoma: A morphological, immunohistochemical, and molecular study. 猫霍奇金样淋巴瘤：形态学、免疫组织化学和分子研究。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-20 DOI: 10.1177/03009858251338852

Greta Foiani, Silvia Dell'Aere, Marta Vascellari, Francesca Tiracorrendo, Gabriele Ghisleni, Antonella Rigillo, Stefano Perfetto, Erica Melchiotti, Antonio Carminato, Gaia B M Bianchi, Paola Roccabianca

{"title":"Feline Hodgkin-like lymphoma: A morphological, immunohistochemical, and molecular study.","authors":"Greta Foiani, Silvia Dell'Aere, Marta Vascellari, Francesca Tiracorrendo, Gabriele Ghisleni, Antonella Rigillo, Stefano Perfetto, Erica Melchiotti, Antonio Carminato, Gaia B M Bianchi, Paola Roccabianca","doi":"10.1177/03009858251338852","DOIUrl":"https://doi.org/10.1177/03009858251338852","url":null,"abstract":"Fourteen feline nodal lymphomas previously diagnosed as Hodgkin-like lymphoma (HLL) were studied through histologic, immunohistochemical, and molecular analyses to further characterize feline HLL. The cohort comprised 12 domestic shorthair and 2 Maine coon cats, with a male/female ratio of 1.3 and a median age of 9.5 years. Reed-Sternberg and Hodgkin cells were observed in 14/14 HLLs, while a minority of cells resembling lymphocyte-predominant cells were observed in 5/14 (36%) cases. Neoplastic cells were embedded in a mixed reactive background mainly composed of T and B lymphocytes and histiocytes. Necrosis was present in 9/14 (64%) cases. Various percentages of neoplastic cells were positive for CD30, PAX5, and MUM1 in 14/14 cases and for CD20 in 12/14 (86%) cases. Polymerase chain reaction (PCR) for antigen receptor rearrangements (PARR) by agarose gel electrophoresis identified clonal immunoglobulin heavy chain (IGH) in 9/14 (64%) cases, clonal T-cell receptor-gamma (TRG) rearrangements in 4/14 (29%) cases, and polyclonal IGH and TRG in 1 case. The predominance of Reed-Sternberg and Hodgkin cells and a CD30+/PAX5+/MUM1+ immunophenotype were consistent findings in this case series and align with the human classic form of Hodgkin lymphoma (HL). However, in contrast to the human tumor, most feline HLLs express CD20. Based on the human HL classification, feline HLLs were further categorized as lymphocyte-rich (6/14, 43%), mixed cellularity (4/14, 29%), nodular sclerosis (2/14, 14%), and lymphocyte-depleted (2/14, 14%). Feline HLL is a complex neoplasm that requires refinement of diagnostic criteria to improve classification and management.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251338852"},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic significance of pSTAT3 expression in feline mammary carcinomas. pSTAT3表达在猫乳腺癌中的预后意义。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-20 DOI: 10.1177/03009858251338851

Keishi Owaki, Mami Murakami, Kentaro Okamoto, Akihiro Hirata, Hiroki Sakai

{"title":"Prognostic significance of pSTAT3 expression in feline mammary carcinomas.","authors":"Keishi Owaki, Mami Murakami, Kentaro Okamoto, Akihiro Hirata, Hiroki Sakai","doi":"10.1177/03009858251338851","DOIUrl":"https://doi.org/10.1177/03009858251338851","url":null,"abstract":"Feline mammary carcinomas (FMCs) have a highly malignant and metastatic behavior and are associated with a poor prognosis. There is a need to identify new effective therapies for FMC. Signal transducer and activator of transcription 3 (STAT3) is activated in various human and animal tumors. It has been proposed as a potential prognostic marker and therapeutic target in human breast cancer (HBC). Given the similarities between HBC and FMCs, we hypothesized STAT3 expression in FMCs may be prognostically significant. In this study, 60 FMC samples were immunohistochemically evaluated for the expression of total STAT3 (tSTAT3), phosphorylated STAT3 (pSTAT3), and Ki-67. The pSTAT3 and Ki-67 indexes were calculated, and their associations with clinicopathological features, as well as tumor-specific survival (TSS), were investigated. Receiver operating characteristic curves were generated to determine the cutoff values for pSTAT3 and Ki-67 using Youden's index as the criterion. All FMCs positively immunolabeled for tSTAT3 and pSTAT3. Significant associations were observed between the pSTAT3 index and the WHO clinical stage, lymph node metastasis, and lymphovascular invasion. However, no significant associations were established with any other factors. Using the cutoff value to classify the pSTAT3 index, the higher pSTAT3 index (>25.2%) was significantly associated with decreased TSS (P = .00570). No significant associations were established between the Ki-67 index and any of the factors. Also, a significant positive association was observed between the pSTAT3 and Ki-67 indexes (P = .0001). This significant association with TSS underscores the potential role of pSTAT3 as a prognostic marker in FMC.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251338851"},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute inhalation toxicity in nine white ibis (Eudocimus albus) exposed to theatrical fog containing triethylene glycol and propylene glycol. 暴露于含有三甘醇和丙二醇的戏剧雾中的9只白朱鹭的急性吸入毒性。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-16 DOI: 10.1177/03009858251338853

Jayne S Ellis, Richard M Fulton, Andreas F Lehner, John P Buchweitz

{"title":"Acute inhalation toxicity in nine white ibis (Eudocimus albus) exposed to theatrical fog containing triethylene glycol and propylene glycol.","authors":"Jayne S Ellis, Richard M Fulton, Andreas F Lehner, John P Buchweitz","doi":"10.1177/03009858251338853","DOIUrl":"https://doi.org/10.1177/03009858251338853","url":null,"abstract":"Nine wild American white ibis (Eudocimus albus) were found deceased or were moribund and subsequently euthanized within 24 hours after exposure to theatrical fog containing propylene glycol and triethylene glycol at a Halloween event at a zoological institution. Gross examinations revealed that all birds had congestion, edema, and hemorrhage throughout the lungs. Histologically, all birds had pathologic changes within the trachea and lungs indicative of acute respiratory insult. Microscopic changes in the trachea included segmental to diffuse epithelial attenuation with loss of cilia, alternating with regions of goblet cells filled with abundant mucus. In the lungs, all birds had perivascular edema and degenerative changes to the epithelium lining primary and secondary bronchi including hypereosinophilia and apical cytoplasmic blebbing of bronchial epithelial cells. In addition, in the lungs of 4 birds with longer intervals between exposure and death, there was granulomatous pneumonia, heterophilic perivascular cuffing, and multifocal bronchial epithelial necrosis. Propylene glycol and triethylene glycol were detected in lung and kidney tissues by gas chromatography-tandem mass spectrometry (GC-MS/MS). Traces of oxalic acid were found, along with presence of glycolic acid. While exposure to aerosolized glycols has been shown to cause irritation and minor degenerative changes to the respiratory epithelium in laboratory animals and humans, this study represents a unique investigation into the first reported incidence of acute inhalation toxicity and death following exposure to aerosolized glycol-containing theatrical fog in birds.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251338853"},"PeriodicalIF":2.3,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sudden death of a cynomolgus monkey due to transfusion-related acute lung injury (TRALI) after xeno-transfusion from a minipig. 一只食蟹猴在接受猪异种输血后，因输血相关急性肺损伤（TRALI）而猝死。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-16 DOI: 10.1177/03009858251338850

Jinhyung Rho, Hyung-Seon Kim, Mi-Jin Yang, Jeong-Ho Hwang, Hwa-Young Son

引用次数: 0

Image challenge in Veterinary Pathology. 兽医病理学中的图像挑战。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-01 Epub Date: 2025-05-05 DOI: 10.1177/03009858241312641

引用次数: 0

Canine synovial lipomatosis: Clinicopathologic findings and HGMA2 immunohistochemistry in 4 cases. 犬滑膜脂肪瘤病4例临床病理及HGMA2免疫组化分析

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-01 Epub Date: 2025-01-02 DOI: 10.1177/03009858241306404

Keiichi Kuroki, Katelin Dark, Jimmy C Lattimer, Gayle C Johnson

引用次数: 0

Immunohistochemical expression of TLR1, TLR2, TLR4, and TLR9 in the different types of lesions associated with bovine paratuberculosis. TLR1、TLR2、TLR4和TLR9在牛副结核相关不同类型病变中的免疫组织化学表达

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-01 Epub Date: 2024-12-25 DOI: 10.1177/03009858241302850

David Zapico, José Espinosa, Miguel Criado, Daniel Gutiérrez, María Del Carmen Ferreras, Julio Benavides, Valentín Pérez, Miguel Fernández

{"title":"Immunohistochemical expression of TLR1, TLR2, TLR4, and TLR9 in the different types of lesions associated with bovine paratuberculosis.","authors":"David Zapico, José Espinosa, Miguel Criado, Daniel Gutiérrez, María Del Carmen Ferreras, Julio Benavides, Valentín Pérez, Miguel Fernández","doi":"10.1177/03009858241302850","DOIUrl":"10.1177/03009858241302850","url":null,"abstract":"The factors that determine the appearance of the different pathologic forms associated with bovine paratuberculosis are not fully understood, but new research suggests a critical role of innate immunity. Toll-like receptors (TLRs) trigger the recognition of invading pathogens by innate immune cells and the onset of specific immune responses. The aim of this work was to assess, immunohistochemically, the expression of TLR1, TLR2, TLR4, and TLR9 in intestinal samples of 20 cows showing different types of paratuberculous lesions: uninfected controls, focal lesions, paucibacillary, and multibacillary diffuse forms. The majority of labeled cells were morphologically consistent with macrophages. A differential cell count was performed in the intestinal lamina propria, gut-associated lymphoid tissue, and mesenteric lymph node. TLR9 immunolabeling between the different types of lesions was compared using a complete H-score. Focal and diffuse paucibacillary forms contained significantly increased TLR2-expressing macrophages outside of the lesions compared with the controls and diffuse multibacillary forms, and moderate TLR9 immunolabeling within granulomas. In the multibacillary granulomatous lesions, the expression of TLR1 and TLR4 was observed as well as increased TLR9 expression compared with the rest of the groups. Differences in the predominance of one type or another of TLR allows us to elucidate the importance of the innate immune response and its possible role in the development of the different types of paratuberculosis lesions.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"305-318"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feline dermoid cyst: Retrospective case series and literature review. 猫皮样囊肿：回顾性病例系列和文献复习。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-01 Epub Date: 2025-02-19 DOI: 10.1177/03009858251317457

Daniel Felipe Barrantes Murillo, Daniel R Rissi, Dominique J Wiener, Tatiane Terumi Negrão Watanabe

{"title":"Feline dermoid cyst: Retrospective case series and literature review.","authors":"Daniel Felipe Barrantes Murillo, Daniel R Rissi, Dominique J Wiener, Tatiane Terumi Negrão Watanabe","doi":"10.1177/03009858251317457","DOIUrl":"10.1177/03009858251317457","url":null,"abstract":"Dermoid cysts are developmental abnormalities usually located at the dorsal body midline. Histologically, these lesions reduplicate the skin, with associated collagen bundles, adnexal structures, and are filled by keratin and hairs. While these cysts have well-recognized breed and anatomical predispositions in dogs, the information in feline patients is restricted to scattered case reports and anecdotal data. Through a multi-institutional retrospective case series, we aim to describe the clinical and demographic features of this condition in cats. We retrieved a total of 220 cases. The average age at the time of diagnosis was 5.5 years, with 59.5% (131/220) being males. Domestic short hairs were the most represented breed, accounting for 56.4% (124/220) of the cases. The average cyst diameter was 1.4 cm, and 99.5% (219/220) of the cases the cysts were cutaneous and subcutaneous with the most common anatomical location being the neck (55.9%; 123/220). One dermoid cyst was in the mesentery. In most cases, no associated inflammatory lesions were reported (66.4%; 146/220). The anatomical location of the dermoid cyst did not differ significantly among sexes (Chi-square test, P = .840), breeds (Chi-square test, P = .999), ages (Chi-square test, P = .627), or other histological findings related to the cyst (Chi-square test, P = .363).","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"293-299"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143450244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic challenge in veterinary pathology: Does negative Ziehl-Neelsen staining rule out mycobacteriosis? 兽医病理学的诊断挑战：ziehl - nielsen染色阴性是否可以排除分枝杆菌病？

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-05-01 Epub Date: 2025-04-02 DOI: 10.1177/03009858251331342

Anna Huupponen, Sini Ulmanen, Thomas Grönthal, Taru Lienemann, Sanna Viitanen, Pernilla Syrjä

引用次数: 0