Veterinary Pathology最新文献

{"title":"Canine laryngotracheal plasma cell tumors: Ten cases and literature review.","authors":"Kathleen R Mulka, Deborah Gillette, Amy C Durham, Elizabeth A Mauldin","doi":"10.1177/03009858251331115","DOIUrl":"10.1177/03009858251331115","url":null,"abstract":"<p><p>Canine extramedullary plasma cell tumors (EMPs) most commonly arise in the skin, oral cavity, rectum, and colon. This retrospective study describes the histopathological and immunohistochemical characteristics and associated clinical signs and outcomes of laryngeal and tracheal EMPs in dogs. Five tracheal and 5 laryngeal EMPs were diagnosed at the Penn Vet Diagnostic Laboratory. Clinical information was obtained via submission forms and follow-up questionnaires. All dogs were male (9 castrated), 7 to 15 years old, and of different breeds. Neoplasms were composed of well-differentiated (n = 6) or moderately differentiated (n = 4) neoplastic plasma cells arranged in sheets, cords, and packets. All neoplasms labeled positively for MUM-1, negatively for PAX5, and were variably CD20- and CD79b-positive. There was no recurrence or disease progression 2 months to 7 years post biopsy in 6/9 cases. Results suggest that surgical resection can result in positive outcomes. Further studies are needed to identify factors leading to progression of this disease.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"692-696"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital quantification of porcine circovirus 2 (PCV-2)-infected cells in lymph nodes of pigs with natural PCV-2 systemic disease shows strong association with manual scoring and quantitative PCR.","authors":"Dávid G Horváth, Lilla Dénes, Barbara Igriczi, Márton Papp, Víctor Hidalgo-Martínez, Joaquim Segalés, Gyula Balka","doi":"10.1177/03009858251331121","DOIUrl":"10.1177/03009858251331121","url":null,"abstract":"<p><p>The diagnosis of porcine circovirus 2 (PCV-2) systemic disease is based on clinical signs, microscopic lesions, and semiquantitative PCV-2 assessment by in situ visualization of immunohistochemistry (IHC) or in situ hybridization labeling within lymphoid tissue lesions. We aimed to digitally quantify IHC-labeled PCV-2-infected cells in porcine lymph nodes with natural PCV-2 systemic disease (<i>n</i> = 53) and to compare these quantitative results with the subjective manual method, and also with the viral DNA copy numbers of the corresponding formalin-fixed, paraffin-embedded tissue blocks. In total, 47 of 54 (87%) cases had the same manual score by 2 independent observers. Infected cell counts increased with manual score severity, and their count could be matched to the manual method. Infected cell odds showed a significant strong positive correlation with the viral copy numbers (ρ = 0.8461, <i>P</i> < .001). Digital image analysis is efficient for PCV-2 IHC signal quantification and can make case assessment more objective and reproducible.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"714-718"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of how colors clue us into gross diagnosis in domestic animals.","authors":"Daniel R Rissi, Ricardo E Mendes, Chloe C Goodwin, Claudio S L Barros","doi":"10.1177/03009858251322738","DOIUrl":"10.1177/03009858251322738","url":null,"abstract":"<p><p>Different tissues have a normal color spectrum that reflects their cellular composition and/or metabolic features. Similarly, distinct color variations may occur in tissues that have undergone pathologic or nonpathologic changes. Common examples of color changes in domestic animal tissues include red (associated with erythrocytes, hemoglobin, and myoglobin), brown (ferric hemoglobin or myoglobin, suppurative inflammation, lipid oxidation, postmortem autolysis, formalin fixation, neoplasms arising from cytochrome-rich tissues), yellow (hemoglobin and iron degradation, biliary pigment and by-products, carotenes, keratin, necrosis, suppurative or fibrinous inflammation), green (hemoglobin and iron degradation, biliary pigment and by-products, meconium, eosinophilic or suppurative inflammation, oomycete and algal infections), white (lack of blood, adipose tissue and its neoplasms, chylous effusion, necrosis, mineralization, fibrosis, lymphoid tissue, round cell neoplasms), translucent (transudate, cysts), black to gray (hemoglobin and iron degradation, melanin, carbon, tattoos), and blue to purple (poorly oxygenated blood, tattoos). Pathologists and pathology trainees can benefit from understanding why particular colors are present in a tissue or organ and are advised to recognize the color dynamics that occur over time, such as hemorrhage progressing from red to purple and subsequently to yellow, green, and brown. Therefore, clear and precise color recognition and description is a key feature of a gross examination. Understanding the relationship between color changes in tissues and the underlying biologic or pathologic processes can help elucidate disease recognition and diagnosis.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"646-658"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ocular and periocular Usutu virus-associated lesions in naturally infected Eurasian blackbirds (<i>Turdus merula</i>).","authors":"Giuseppe Giglia, Gianfilippo Agliani, Nadiah M H A van Eijk, Erwin de Bruin, Christine Fast, Reina S Sikkema, Bas B Oude Munnink, Maria Teresa Mandara, Andrea Gröne, Judith M A van den Brand","doi":"10.1177/03009858251324640","DOIUrl":"10.1177/03009858251324640","url":null,"abstract":"<p><p>Usutu virus (USUV) is a zoonotic neurotropic arbovirus related to the West Nile virus that causes mortality in birds and sporadic neurologic human disease. Current research on natural USUV-associated disease lacks data on ocular involvement. This study investigated ocular and periocular tissue involvement in natural USUV infections and associated disease in Eurasian blackbirds (<i>Turdus merula</i>). Twenty-two found-dead Eurasian blackbirds were examined. USUV reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) detected 12/22 infected blackbirds. Histology and immunohistochemistry for virus antigen, inflammation (anti-CD3 for T-lymphocytes), and apoptosis (anti-cleaved caspase-3 (CC3)) assessed the virus tropism and associated damage. In the eye of USUV-infected blackbirds, choroiditis was the main finding (9/12), while the pecten oculi (4/12) and optic nerves (4/12) were occasionally affected. Virus antigen was detected in the lesions. The cornea and retina lacked virus antigens and lesions. Periocular soft tissues (12/12) and eyelids (9/12) were also affected. Lesions in the choroid (<i>P</i> < .001), ciliary bodies (<i>P</i> < .01), and sclera (<i>P</i> < .05) were significantly associated with USUV infection. In the choroid, CD3 and CC3 strongly correlated with the virus antigen scores (<i>P</i> < .0001), suggesting a T-cell response and apoptosis involvement in the ocular damage. A negative correlation was identified for the virus antigen score in choroid and eyelids with USUV RT-qPCR Ct values (<i>P</i> < .05). This study reports for USUV features consistent with ocular and periocular tropism and disease with a major involvement of the choroid, suggesting a primary ocular vascular spread.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"773-780"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"World-class educational content and award-winning presentations await you at the 2025 ACVP annual meeting.","authors":"Katharine Webster","doi":"10.1177/03009858251356601","DOIUrl":"https://doi.org/10.1177/03009858251356601","url":null,"abstract":"","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":"62 5","pages":"618-619"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sudden death of a cynomolgus monkey due to transfusion-related acute lung injury (TRALI) after xeno-transfusion from a minipig.","authors":"Jinhyung Rho, Hyung-Seon Kim, Mi-Jin Yang, Jeong-Ho Hwang, Hwa-Young Son","doi":"10.1177/03009858251338850","DOIUrl":"10.1177/03009858251338850","url":null,"abstract":"<p><p>Blood transfusion in veterinary science is vital but has not been thoroughly investigated due to the diversity and complexity between species. Here, we introduce the case of a cynomolgus monkey that exhibited loss of pupil reflex and died 1 hour after xeno-transfusion with triple-knockout red blood cells from a minipig. A full necropsy was performed to investigate the cause of death. Gross observations revealed foamy and bloody exudate in the mouth; however, no signs of injury were observed in the upper respiratory tract. The lung weight was nearly 5 times higher than that of similar-aged monkeys. The texture of the lung was sand-like, but red and dark red discoloration was observed throughout the lung. Histological examination revealed restricted hemorrhage in the perivascular region in the lungs and phagocytosis of red blood cells by neutrophils in the liver. Thus, the lesion corresponded to a transfusion-related acute lung injury.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"802-805"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reporting guidelines for manuscripts that use artificial intelligence-based automated image analysis in <i>Veterinary Pathology</i>.","authors":"Christof A Bertram, Melissa Schutten, Lorenzo Ressel, Katharina Breininger, Joshua D Webster, Marc Aubreville","doi":"10.1177/03009858251344320","DOIUrl":"https://doi.org/10.1177/03009858251344320","url":null,"abstract":"","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":"62 5","pages":"615-617"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The golden Syrian hamster (<i>Mesocricetus auratus</i>) as a model to decipher relevant pathogenic aspects of sheep-associated malignant catarrhal fever.","authors":"Rosalie Fabian, Eleanor G Bentley, Adam Kirby, Parul Sharma, James P Stewart, Anja Kipar","doi":"10.1177/03009858251315115","DOIUrl":"10.1177/03009858251315115","url":null,"abstract":"<p><p>Malignant catarrhal fever (MCF) is an often fatal, sporadic gammaherpesvirus-induced disease of ruminants with global relevance. Ovine gammaherpesvirus-2 (OvHV-2), with sheep as its reservoir host, is a major cause of MCF in susceptible species. Despite extensive research on the molecular aspects of the disease, its pathogenesis is not yet fully understood. The present study re-established the Syrian golden hamster (<i>Mesocricetus auratus</i>) as an amenable animal model of MCF and applied complementary in situ approaches to confirm recent findings in natural disease that could shed new light on pathogenic aspects of MCF. These showed that systemic OvHV-2 infection is associated with T-cell and macrophage-dominated mononuclear infiltrates and vasculitis in various organs. Both T-cells and monocytes/macrophages harbor the virus, and infected leukocytes are abundant in the infiltrates. The results also indicate that OvHV-2 has a broader target cell spectrum, including vascular endothelial cells and selected squamous epithelia. The former supports the interpretation that the inflammatory processes develop due to circulating, activated, infected T-cells and monocytes that home to tissues and emigrate from vessels prone to leukocyte emigration, possibly with direct interaction between virus-infected leukocytes and endothelial cells. The latter supports the hypothesis of graft versus host disease scenario, without viral cytopathic effect on epithelial cells but infiltration of the mucosa by infected T-cells and macrophages. The disease processes are accompanied by evidence of expansion of the T-cell compartments and the monocyte/macrophage pool in lymphatic tissues and bone marrow.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"697-713"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereotypic immune response in <i>Mycobacterium avium</i> ssp. <i>paratuberculosis</i> infection among different Swiss caprine genotypes.","authors":"Melina Rasper-Hössinger, Simone Scherrer, Roger Stephan, Frauke Seehusen","doi":"10.1177/03009858251322726","DOIUrl":"10.1177/03009858251322726","url":null,"abstract":"<p><p>Paratuberculosis is an infection with <i>Mycobacterium avium</i> ssp. <i>paratuberculosis</i> (MAP) causing chronic enteritis in domestic and wild ruminants worldwide. In goats, the infection is caused by C (cattle)-type and S (sheep)-type strains. In this study, the correlation between different MAP strains and histomorphological lesions in the small and large intestines, as well as the mesenteric lymph nodes, in Swiss goats (<i>Caprae aegagrus hircus</i>) was investigated. Ten Swiss caprine MAP isolates were characterized using polymerase chain reaction (PCR) and enzymatic restriction-based single nucleotide polymorphism (SNP) analysis. In addition, mycobacterial interspersed repetitive units and variable-number tandem repeats (MIRU-VNTR) profiling was performed, and the correlation with histologic lesions, scored as previously described for goats, was analyzed. Furthermore, immunohistochemical expression of CD3, CD79a, Iba1, cleaved caspase 3, and interleukin (IL)-17 was evaluated, and a morphometric analysis was conducted to quantify the different inflammatory cells. Diffuse multibacillary lesions were found in C-type/L'Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE) Nouzilly MIRU-VNTR (INMV)1 (2/10) and S-type/INMV220 (1/10) animals. Diffuse lymphocytic lesions occurred in C-type/INMV1 (2/10) animals, while diffuse mixed lesions were observed in S-type/INMV218 (3/10) and S-type/INMV220 (2/10) animals. No significant differences in intestinal histological lesion scores were detected between S- and C-type INMV strains. Morphometrical analysis revealed similar inflammatory and apoptotic cell numbers in the intestinal mucosa of C- and S-type animals; however, S-type animals exhibited significantly more Iba1- and cleaved caspase 3-positive cells in mesenteric lymph nodes. Lesions in mesenteric lymph nodes might indicate a differentially regulated course in MAP pathogenesis.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"728-739"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue expression and urinary excretion of liver-type fatty acid binding protein in older dogs with or without early signs of chronic kidney disease.","authors":"Sofie Marynissen, Kristel Demeyere, Sylvie Daminet, Evelyne Meyer, Koen Chiers, Dominique Paepe","doi":"10.1177/03009858251315106","DOIUrl":"10.1177/03009858251315106","url":null,"abstract":"<p><p>Liver-type fatty acid-binding protein (L-FABP) is expressed by several tissues, plays a role in fatty acid metabolism, and has antioxidant effects. Its renal expression is upregulated by stress. Urinary L-FABP (uL-FABP) is a promising kidney biomarker in people for detection of early acute and chronic kidney disease (CKD) and as a marker for progression in patients with glomerulonephritis. However, data on canine uL-FABP are currently limited. This prospective study was designed to examine canine tissue expression of L-FABP and to validate an ELISA to quantify uL-FABP in older dogs with or without early signs of CKD. Tissues of 4 recently euthanized dogs and 117 urine samples of 73 client-owned older dogs undergoing health screening were evaluated in the study. Immunohistochemistry was performed on kidney and liver tissues. Analytical validation of a commercially available ELISA for measurement of L-FABP in canine urine was performed (limit of detection, imprecision, specificity). The ELISA was used to measure L-FABP in stored urine samples from a cohort of older dogs. Dogs were found to express L-FABP, mostly in proximal tubular epithelial cells and in the periportal hepatocytes of the liver. Assay validation revealed poor sensitivity and imprecision for measurement of canine uL-FABP. Of the 117 urine samples analyzed, 98 were below the limit of detection (LOD; 7.30 ng/mL) and a further 5 were below the limit of quantification (LOQ; 16.20 ng/mL). The proximal tubules of dog kidneys express L-FABP, but the value of uL-FABP as tubular marker in older dogs warrants further study.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"719-727"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}