Veterinary Pathology最新文献

{"title":"Incidence, gross features, and histologic characterization of feline fibrocystic hepatobiliary lesions and extrahepatic biliary malformations.","authors":"Megan E Schreeg, John M Cullen","doi":"10.1177/03009858251322737","DOIUrl":"10.1177/03009858251322737","url":null,"abstract":"<p><p>Feline fibrocystic hepatobiliary lesions (FHLs) and extrahepatic biliary malformations (EBMs) are supposedly common, but prevalence studies and detailed gross and histologic descriptions within a large cohort are lacking. Our aims were to identify the prevalence of FHLs and EBMs within a large feline population, characterize gross and histologic features, and report comorbidities. Of 140 cats, 20 (14.3%) had either FHLs and/or EBMs. FHLs were most common (15/20, 75%) and included cystic biliary lesions (CBLs, 14/20, 70%) and congenital hepatic fibrosis (CHF, 1/20, 5%). CBLs had gross variability but histologically comprised multiloculated cysts lined by biliary epithelium overlying variably hyalinized collagenous trabeculae. Gross features of CHF included severe hepatic fibrosis with embedded biliary cysts, parenchymal collapse, and nodular regeneration. Histologically, there was loss of lobular architecture, proliferation of small irregular to cystic bile ducts, bridging fibrosis, inapparent portal veins, and scattered hepatocellular necrosis. Gallbladder malformations were present in 5/20 cats (25%) and were classified as duplex (2/5) or bilobed (3/5). Two of 20 cats (10%) had anomalous, blind-ended, extrahepatic bile ducts extending from the gallbladder into the pancreas/peripancreatic mesentery. FHLs and EBMs were rarely concurrent (1/20 cats, 5%). CHF contributed to significant morbidity, but other FHLs/EBMs were incidental. Concurrent pancreatic/renal cysts were uncommon (2/20, 10%) and were seen in association with a CBL and CHF. Common comorbidities included cardiovascular and/or renal disease and neoplasia. We provide evidence that the majority of feline FHLs represent ductal plate malformations, with CBLs having morphologic features compatible with von Meyenburg complexes (VMCs)/biliary hamartomas.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"748-761"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histologic spectrum of canine extramedullary plasmacytomas: Diagnostic challenges and immunohistochemical differentiation from cutaneous histiocytomas.","authors":"Bartolomeu B N Santos, Paulo V Peixoto, Maria C Peleteiro, Daniel R Rissi, Sandra Carvalho, Ticiana N França","doi":"10.1177/03009858251361141","DOIUrl":"https://doi.org/10.1177/03009858251361141","url":null,"abstract":"<p><p>Here we assess the diagnostic features of 73 canine extramedullary plasmacytomas (EMPs) and their differentiation from canine cutaneous histiocytomas (CHs) using histology and immunohistochemistry for MUM1, CD3, CD79a, immunoglobulin (Ig) light chains (λ and κ), and IBA1. Most EMPs were classified as cleaved and mature subtypes. Almost 92% of EMPs were positive for MUM1, whereas 77% were positive for CD79a and 74% for Ig light chains λ and κ. The use of MUM1 and Ig light chains resulted in a sensitivity of 100% in diagnosing EMPs, surpassing the combination of MUM1 and CD79a (94%). In 8% of the EMPs, there was pseudofollicular arrangement of neoplastic cells, whereas 7% had amyloid deposition and 3% had mineralization. CHs were positive for IBA1 but negative for all other IHCs.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251361141"},"PeriodicalIF":1.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A standardized protocol for assessing immunodeficiency in mouse models.","authors":"Andrea Cappelleri, Simone Canesi, Valentina Capo, Alessandra Zecchillo, Luigi D Notarangelo, Elena Draghici, Virginia Bettoni, Valeria Martini, Pierangelo Moretti, Anna Villa, Saverio Paltrinieri, Eugenio Scanziani, Camilla Recordati","doi":"10.1177/03009858251361517","DOIUrl":"10.1177/03009858251361517","url":null,"abstract":"<p><p>Immunodeficient mouse strains are widely used in several fields of biomedical research. Despite that, no standardized system for evaluating immunodeficiency in mice currently exists, and an unbiased comparison of various immunodeficient mouse strains is difficult. The aim of our study was to develop a standardized multi-disciplinary protocol for the morpho-phenotypical assessment of immunodeficient mouse models. We selected 4 immunodeficient strains of mice (<i>Cd40l</i>^-/-, <i>Was</i>^-/-, <i>Rag1</i><i>^R972Q/R972Q</i>, and <i>Rag1</i>^-/-) on a C57BL/6J genetic background and a group of control C57BL/6J wild-type mice. The lymphoid organs were harvested, weighed, and analyzed by histology, immunohistochemistry, and flow cytometry. Hematology and bone marrow cytology were also performed. The main immune cell populations were investigated, including lymphocytes, monocytes/macrophages, neutrophils, and natural killer cells. Relative organ weights were lower in the strains with the highest level of immunodeficiency (<i>Rag1</i><i>^R972Q/R972Q</i> and <i>Rag1</i>^-/-). Histology revealed overall lower cellularity in the same strains, particularly in <i>Rag1</i>^-/- mice. Tissue spatial distributions of the immune cell populations were confirmed by immunohistochemistry, while flow cytometry allowed for their relative quantification. Likewise, hematology detected moderate lymphopenia in the <i>Rag1</i><i>^R972Q/R972Q</i> mice and more severe lymphopenia in <i>Rag1</i>^-/- mice. Our protocol has proven itself effective for the morpho-phenotypical assessment of the immunodeficient mouse models under investigation and was useful in characterizing the type and severity of the defects. The different laboratory techniques were consistent in the characterization and confirmation of immunodeficiency in the different strains, providing different complementary insights.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251361517"},"PeriodicalIF":1.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in feline perianesthetic death postmortem submissions from a referral teaching hospital and general practices in Saskatchewan (Canada) and a proposed postmortem checklist.","authors":"Nicole Rose, Bruce K Wobeser, Jennifer L Davies, Daniel J Pang","doi":"10.1177/03009858251361134","DOIUrl":"https://doi.org/10.1177/03009858251361134","url":null,"abstract":"<p><p>Lesions associated with perianesthetic death (PAD) postmortem submissions are infrequently reported in the literature, with no studies comparing findings between general and referral practices (RPs). This study compared PAD postmortem submissions in cats from a referral teaching hospital (referral practice, RP) and general practices (GP) in Saskatchewan. In the RP, death was most commonly due to euthanasia (15/23, 65%), with most cases having severe underlying disease. In GP, most deaths were unassisted (37/45, 82%), and most animals (33/37, 89%) had an undiagnosed condition or an unknown cause of death. The American Society of Anesthesiologists (ASA) physical status classification was high (ASA III-V) in 16/23 (70%) of RP cases and low (ASA I-II) in 38/45 (85%) of GP cases. Cats with limited medical history accounted for 5/23 (22%) of the RP submissions and 17/45 (38%) of the GP submissions. Reporting of gross examination findings and tissues collected for histologic examination were inconsistent. For example, although the presence of negative pressure within the thoracic cavity is evaluated routinely during a complete postmortem examination, its presence (or absence) was only reported in 4/45 (9%) of cases where the animal died unassisted. No significant difference was found in determining the cause of death between RP and GP when euthanized cases were excluded (<i>P</i> = .445). A standardized perianesthetic postmortem checklist is proposed to enhance reporting and improve diagnostic consistency.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251361134"},"PeriodicalIF":1.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Errors in pathology: The real world.","authors":"Jerrold M Ward, Jeffrey C Wolf, John P Sundberg","doi":"10.1177/03009858251367406","DOIUrl":"10.1177/03009858251367406","url":null,"abstract":"","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251367406"},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural tick-borne encephalitis in 2 Huacaya alpacas (<i>Vicugna pacos</i>).","authors":"Denise Thaller, Claudia Schulz, Angelika Auer, Zoltán Bagó, Sandra Revilla-Fernández, Michael D Mansfeld, Kaspar Matiasek, Andrea Klang","doi":"10.1177/03009858251362432","DOIUrl":"10.1177/03009858251362432","url":null,"abstract":"<p><p>Although rare, tick-borne encephalitis (TBE) is one of the most important and commonly fatal viral diseases affecting the central nervous system (CNS). This arboviral disease is transmitted by ticks and prevalent in widespread parts of Eurasia. Besides humans, several domestic animals such as dogs, horses, and ruminants can also be infected. To our knowledge, there have been no reports of TBE in South American camelids, so far. Here, we present 2 cases of Huacaya alpacas with progressive, therapy-resistant neurologic signs, which were euthanized and submitted for necropsy. Histologic examination of the CNS revealed a moderate lymphohistiocytic meningoencephalomyelitis characterized by perivascular cuffing, glial cell proliferation, neuronal degeneration, and neuronophagia. Tick-borne encephalitis virus (TBEV) infection was confirmed by polymerase chain reaction (PCR), sequencing, immunohistochemistry, and RNAscope in situ hybridization. TBEV should be included as a differential diagnosis in alpacas from endemic regions presenting with neurologic signs.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251362432"},"PeriodicalIF":1.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphological and ultrastructural characterization of pancreatic islet intranuclear and intracytoplasmic inclusions in giant southern pouched rats (<i>Cricetomys ansorgei</i>).","authors":"Sophie Nelissen, Elizabeth S Lavin, Aníbal G Armién, Elena A Demeter","doi":"10.1177/03009858251361140","DOIUrl":"https://doi.org/10.1177/03009858251361140","url":null,"abstract":"<p><p>Intracellular inclusions are singular structures that may occur secondary to viral infection, cytoplasmic invagination, and organelle entrapment, or due to abnormal accumulation of biological material, such as proteins. Determining the exact nature of an inclusion is crucial in diagnostic pathology, especially in the context of colony management and toxicity studies. In this case series, we identified pancreatic islet intranuclear (IN) and intracytoplasmic (IC) eosinophilic inclusions in 13 out of 21 southern giant pouched rats (<i>Cricetomys ansorgei</i>), a species studied for its outstanding olfactory capacities. Intranuclear inclusions were smooth, globular, and marginated the chromatin. Intracytoplasmic inclusions were either single or multiple, and polygonal to globular. Females and males were both affected, regardless of their wild-caught or inhouse-bred status. Immunohistochemistry labeling for p62 in some IN inclusions suggested a correlation with autophagy. Okajima's stain for hemoglobin positively stained all inclusions. Periodic acid-Schiff reaction, Masson's trichrome, Congo red, and Prussian blue were all negative, ruling out polysaccharides, β-pleated sheets, fibrin, and free iron. Ultrastructural evaluation further revealed that IN inclusions consisted of aggregated fibrillar to microtubular material and excluded a viral infection. By contrast, IC inclusions were identified as giant mitochondria with crystalline deposits and abnormal cristae. In conclusion, the frequent occurrence of either type of inclusion, irrespective of clinical health status, suggests that they are likely incidental although possibly related to autophagy. Importantly, the natural occurrence of giant mitochondria in seemingly healthy individuals is unusual. Giant pouched rats may thus represent a suitable species to deepen our understanding of these peculiar organelles.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251361140"},"PeriodicalIF":1.7,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral tenosynovitis in poultry: Integrating histopathology, in situ hybridization, and quantitative reverse transcriptase-PCR for avian reovirus diagnostic workflow.","authors":"Cheng-Shun Hsueh, Pablo E Piñeyro, Olufemi Fasina, Trevor Arunsiripate, Mohamed M El-Gazzar, Yuko Sato","doi":"10.1177/03009858251361516","DOIUrl":"https://doi.org/10.1177/03009858251361516","url":null,"abstract":"<p><p>Avian reovirus (ARV), the etiologic agent of poultry viral arthritis/tenosynovitis, frequently presents diagnostic challenges due to its non-specific lesions, ubiquitous nature, and the lack of standardized diagnostic guidelines. We describe cases of poultry arthritis/tenosynovitis, where ARV was the suspected etiology, and investigate the relationship between lesion severity and viral RNA levels using quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) with RNAScope in situ hybridization (ISH) to support ARV detection and analysis. A total of 51 cases (qRT-PCR positive [<i>n</i> = 38; Ct range = 20.9-35.9] and qRT-PCR negative [<i>n</i> = 13; Ct > 37]) were analyzed, with case selection criteria including clinical lameness, complete histologic examination of the gastrocnemius/digital flexor tendons, and qRT-PCR testing for ARV. A subset of qRT-PCR positive cases (<i>n</i> = 33) and negative cases (<i>n</i> = 8) with the sections showing the most severe histologic lesions were selected for ISH. Histologic scoring of tenosynovitis included inflammation severity; synovial proliferation; and the presence of lymphoid nodules, neovascularization, and fibrosis. The qRT-PCR positive cases had significantly higher histologic scores than negative cases. The ISH detected viral transcripts within synoviocytes and subintimal fibroblasts only in qRT-PCR positive cases (11/33, 33.3%; Ct range = 20.9-32.3). Positive ISH results were also statistically associated with lower Ct values and higher lesion scores. In conclusion, this study aids in ARV diagnostic challenges by linking lesion severity with viral transcription, identifying fibroblasts as ARV-infected cells, and demonstrating ISH as both a valuable diagnostic tool and a means to studying ARV pathogenesis in poultry.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251361516"},"PeriodicalIF":1.7,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144856470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of SOX-10 in identifying nodal metastasis in canine malignant melanoma.","authors":"Silvia Sabattini, Riccardo Zaccone, Gaia Dondi, Eugenio Faroni, Andrea Renzi, Elena Adulti, Giuliano Bettini, Luciana Giardino, Laura Marconato","doi":"10.1177/03009858251362430","DOIUrl":"https://doi.org/10.1177/03009858251362430","url":null,"abstract":"<p><p>Diagnosing early nodal metastases in canine malignant melanoma is challenging due to the morphological similarities between nodal melanophages and neoplastic melanocytes, and the limitations of conventional immunohistochemical markers like melan-A, which only labels a subset of tumor cells and requires tissue bleaching. This retrospective study investigated the utility of the immunohistochemical marker SOX-10, a nuclear transcription factor, in identifying metastatic cells in lymph nodes (LNs) from dogs with oral, labial, or digital malignant melanoma undergoing regional or sentinel lymphadenectomy. The analysis included 49 LNs from 27 dogs with oral (n = 10), labial (n = 9), and digital (n = 8) melanoma. Primary tumors were highly melanotic in 7 (26%) dogs, sparsely melanotic in 15 (56%), and amelanotic in 5 (19%). SOX-10 immunohistochemistry increased the detection rate of nodal metastasis from 29% (14/49 nodes) with hematoxylin and eosin staining alone and 31% (15/49 nodes) with melan-A immunohistochemistry to 33% (16/49 nodes), allowing the identification of 12 LNs with macrometastases, 2 with micrometastases, and 2 with isolated tumor cells. Compared with melan-A, SOX-10 exhibited a more uniform labeling pattern, enhancing the identification of micrometastases and isolated tumor cells. It also facilitated the distinction between neoplastic cells and melanophages, even in heavily pigmented samples, without the need for bleaching, thereby preserving tissue integrity. These findings suggest that SOX-10 is a promising diagnostic marker for canine melanoma, offering improved detection of early melanocytic metastatic lesions.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251362430"},"PeriodicalIF":1.7,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-infection with <i>Vibrio harveyi</i> and monogenean parasites in Malabar groupers (<i>Epinephelus malabaricus</i>).","authors":"Soma Ito, Kazumoto Shibuya, Nayu Kawashima, Nobuyuki Tsutsumi, Katsuaki Sugiura","doi":"10.1177/03009858251362435","DOIUrl":"https://doi.org/10.1177/03009858251362435","url":null,"abstract":"<p><p>A high mortality event occurred among Malabar groupers (<i>Epinephelus malabaricus</i>) reared for research. The affected fish had scattered reddish patches and ulcers on the skin, accompanied by parasite infestations on the skin surface. Histologic findings included ulcerative dermatitis and keratoconjunctivitis with gram-negative bacilli, and the parasites were often observed on the skin surface. Bacterial examinations and <i>in situ</i> hybridization revealed the presence of <i>Vibrio harveyi</i> in the affected tissues. The parasites were morphologically identified as the subfamily Benedeniinae (Monogenea: Capsalidae), likely <i>Neobenedenia girellae</i>. The monogeneans may have come into contact with the fish at a farm in Okinawa, Japan, and potentially facilitated the opportunistic infection with <i>V. harveyi</i>. This is the first report of vibriosis caused by <i>V. harveyi</i> in Malabar groupers.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251362435"},"PeriodicalIF":1.7,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}