Veterinary Pathology最新文献_第6页

Stereotypic immune response in Mycobacterium avium ssp. paratuberculosis infection among different Swiss caprine genotypes. 鸟分枝杆菌的刻板免疫反应。不同瑞士山羊基因型间的副结核感染。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-17 DOI: 10.1177/03009858251322726

Melina Rasper-Hössinger, Simone Scherrer, Roger Stephan, Frauke Seehusen

{"title":"Stereotypic immune response in Mycobacterium avium ssp. paratuberculosis infection among different Swiss caprine genotypes.","authors":"Melina Rasper-Hössinger, Simone Scherrer, Roger Stephan, Frauke Seehusen","doi":"10.1177/03009858251322726","DOIUrl":"https://doi.org/10.1177/03009858251322726","url":null,"abstract":"Paratuberculosis is an infection with Mycobacterium avium ssp. paratuberculosis (MAP) causing chronic enteritis in domestic and wild ruminants worldwide. In goats, the infection is caused by C (cattle)-type and S (sheep)-type strains. In this study, the correlation between different MAP strains and histomorphological lesions in the small and large intestines, as well as the mesenteric lymph nodes, in Swiss goats (Caprae aegagrus hircus) was investigated. Ten Swiss caprine MAP isolates were characterized using polymerase chain reaction (PCR) and enzymatic restriction-based single nucleotide polymorphism (SNP) analysis. In addition, mycobacterial interspersed repetitive units and variable-number tandem repeats (MIRU-VNTR) profiling was performed, and the correlation with histologic lesions, scored as previously described for goats, was analyzed. Furthermore, immunohistochemical expression of CD3, CD79a, Iba1, cleaved caspase 3, and interleukin (IL)-17 was evaluated, and a morphometric analysis was conducted to quantify the different inflammatory cells. Diffuse multibacillary lesions were found in C-type/L'Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE) Nouzilly MIRU-VNTR (INMV)1 (2/10) and S-type/INMV220 (1/10) animals. Diffuse lymphocytic lesions occurred in C-type/INMV1 (2/10) animals, while diffuse mixed lesions were observed in S-type/INMV218 (3/10) and S-type/INMV220 (2/10) animals. No significant differences in intestinal histological lesion scores were detected between S- and C-type INMV strains. Morphometrical analysis revealed similar inflammatory and apoptotic cell numbers in the intestinal mucosa of C- and S-type animals; however, S-type animals exhibited significantly more Iba1- and cleaved caspase 3-positive cells in mesenteric lymph nodes. Lesions in mesenteric lymph nodes might indicate a differentially regulated course in MAP pathogenesis.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251322726"},"PeriodicalIF":2.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic bronchitis and bronchiolitis with prominent globule leukocyte infiltration in a cat with Filobacterium felis. 猫丝状杆菌感染的慢性支气管炎和细支气管炎伴明显的白细胞浸润。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-15 DOI: 10.1177/03009858251324646

Takanori Shiga, Yoko Kakinuma, Miu Takada, Rie Imai, Takuma Aoki, Hiroho Ishida, Miwako Kasahara-Kamiie, Naoyuki Aihara, Junichi Kamiie

{"title":"Chronic bronchitis and bronchiolitis with prominent globule leukocyte infiltration in a cat with Filobacterium felis.","authors":"Takanori Shiga, Yoko Kakinuma, Miu Takada, Rie Imai, Takuma Aoki, Hiroho Ishida, Miwako Kasahara-Kamiie, Naoyuki Aihara, Junichi Kamiie","doi":"10.1177/03009858251324646","DOIUrl":"https://doi.org/10.1177/03009858251324646","url":null,"abstract":"A 7-year-old mixed-breed cat presented with intermittent dry cough for 2 years. Histological examination of the lung biopsy revealed hyperplasia of bronchial and bronchiolar epithelium, and intraepithelial infiltration of globule leukocytes in many bronchi, bronchioles, and terminal bronchioles. Lymphoid aggregates cuffed airways, respiratory bronchioles, and alveolar ducts. Terminal airway inflammation was associated with type II pneumocyte hyperplasia within adjacent alveoli. Warthin-Starry stain revealed numerous argyrophilic and filamentous bacilli that were interspersed or clustered with cilia of the respiratory epithelium. The morphology, distribution, and gram (negative) and ultrastructural characteristics were consistent with cilia-associated respiratory (CAR) bacillus. Real-time polymerase chain reaction assays using paraffin-embedded lung tissue confirmed the presence of Filobacterium felis. Chronic bronchitis and bronchiolitis in a cat with F. felis share characteristics of diseases caused by species specific CAR bacilli in many species, but uniquely in this case, globule leukocytes were a prominent feature of the inflammatory response.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251324646"},"PeriodicalIF":2.3,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of how colors clue us into gross diagnosis in domestic animals. 回顾一下家畜的颜色是如何引导我们进行大体诊断的。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-12 DOI: 10.1177/03009858251322738

Daniel R Rissi, Ricardo E Mendes, Chloe C Goodwin, Claudio S L Barros

{"title":"A review of how colors clue us into gross diagnosis in domestic animals.","authors":"Daniel R Rissi, Ricardo E Mendes, Chloe C Goodwin, Claudio S L Barros","doi":"10.1177/03009858251322738","DOIUrl":"https://doi.org/10.1177/03009858251322738","url":null,"abstract":"Different tissues have a normal color spectrum that reflects their cellular composition and/or metabolic features. Similarly, distinct color variations may occur in tissues that have undergone pathologic or nonpathologic changes. Common examples of color changes in domestic animal tissues include red (associated with erythrocytes, hemoglobin, and myoglobin), brown (ferric hemoglobin or myoglobin, suppurative inflammation, lipid oxidation, postmortem autolysis, formalin fixation, neoplasms arising from cytochrome-rich tissues), yellow (hemoglobin and iron degradation, biliary pigment and by-products, carotenes, keratin, necrosis, suppurative or fibrinous inflammation), green (hemoglobin and iron degradation, biliary pigment and by-products, meconium, eosinophilic or suppurative inflammation, oomycete and algal infections), white (lack of blood, adipose tissue and its neoplasms, chylous effusion, necrosis, mineralization, fibrosis, lymphoid tissue, round cell neoplasms), translucent (transudate, cysts), black to gray (hemoglobin and iron degradation, melanin, carbon, tattoos), and blue to purple (poorly oxygenated blood, tattoos). Pathologists and pathology trainees can benefit from understanding why particular colors are present in a tissue or organ and are advised to recognize the color dynamics that occur over time, such as hemorrhage progressing from red to purple and subsequently to yellow, green, and brown. Therefore, clear and precise color recognition and description is a key feature of a gross examination. Understanding the relationship between color changes in tissues and the underlying biologic or pathologic processes can help elucidate disease recognition and diagnosis.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858251322738"},"PeriodicalIF":2.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Encephalomyelitis and serositis caused by Chlamydia pecorum in buffalo calves from Brazil. 巴西水牛犊由啄木鸟衣原体引起的脑脊髓炎和血清炎。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-01 Epub Date: 2024-10-10 DOI: 10.1177/03009858241288116

Larissa L de Souza, Saulo P Pavarini, Marcele B Bandinelli, Nicole Borel, Rayane C Pupin, Carlos A N Ramos, Ricardo A A Lemos, Danilo C Gomes

{"title":"Encephalomyelitis and serositis caused by Chlamydia pecorum in buffalo calves from Brazil.","authors":"Larissa L de Souza, Saulo P Pavarini, Marcele B Bandinelli, Nicole Borel, Rayane C Pupin, Carlos A N Ramos, Ricardo A A Lemos, Danilo C Gomes","doi":"10.1177/03009858241288116","DOIUrl":"10.1177/03009858241288116","url":null,"abstract":"Chlamydia pecorum causes subclinical infections in cattle, but sporadic, bovine encephalomyelitis cases have been reported in calves and documented in two instances in European buffalo. An outbreak of Chlamydia pecorum-induced encephalomyelitis and serositis occurred in 3-month-old buffalo calves from Brazil. Initially presenting with pelvic limb incoordination, the calves progressed to lateral recumbency, depression, and death. Necropsies of two calves revealed encephalomyelomalacia, fibrin deposition on the external surface of the pericardium (case 1) and pleural and pericardial fibrosis (case 2). Microscopically, a multifocal to coalescing, necrotizing, neutrophilic and lymphocytic meningoencephalomyelitis with fibrinoid vasculitis and thrombosis was present. Anti-Chlamydia antibody labeling was demonstrated by immunohistochemistry. Bacteriological examination yielded no pathogenic bacteria in the brain or lungs. Chlamydia pecorum was confirmed by PCR. This work describes the gross, histopathological, microbiological, and molecular findings in two cases from an outbreak of Chlamydia pecorum-induced disease in buffalo calves.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"221-225"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence can be trained to predict c-KIT-11 mutational status of canine mast cell tumors from hematoxylin and eosin-stained histological slides. 通过训练人工智能，可从苏木精和伊红染色的组织学切片中预测犬肥大细胞瘤的 c-KIT-11 突变状态。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-01 Epub Date: 2024-10-18 DOI: 10.1177/03009858241286806

Chloé Puget, Jonathan Ganz, Julian Ostermaier, Thomas Conrad, Eda Parlak, Christof A Bertram, Matti Kiupel, Katharina Breininger, Marc Aubreville, Robert Klopfleisch

{"title":"Artificial intelligence can be trained to predict c-KIT-11 mutational status of canine mast cell tumors from hematoxylin and eosin-stained histological slides.","authors":"Chloé Puget, Jonathan Ganz, Julian Ostermaier, Thomas Conrad, Eda Parlak, Christof A Bertram, Matti Kiupel, Katharina Breininger, Marc Aubreville, Robert Klopfleisch","doi":"10.1177/03009858241286806","DOIUrl":"10.1177/03009858241286806","url":null,"abstract":"Numerous prognostic factors are currently assessed histologically and immunohistochemically in canine mast cell tumors (MCTs) to evaluate clinical behavior. In addition, polymerase chain reaction (PCR) is often performed to detect internal tandem duplication (ITD) mutations in exon 11 of the c-KIT gene (c-KIT-11-ITD) to predict the therapeutic response to tyrosine kinase inhibitors. This project aimed at training deep learning models (DLMs) to identify MCTs with c-KIT-11-ITD solely based on morphology. Hematoxylin and eosin (HE) stained slides of 368 cutaneous, subcutaneous, and mucocutaneous MCTs (195 with ITD and 173 without) were stained consecutively in 2 different laboratories and scanned with 3 different slide scanners. This resulted in 6 data sets (stain-scanner variations representing diagnostic institutions) of whole-slide images. DLMs were trained with single and mixed data sets and their performances were assessed under stain-scanner variations (domain shifts). The DLM correctly classified HE slides according to their c-KIT-11-ITD status in up to 87% of cases with a 0.90 sensitivity and a 0.83 specificity. A relevant performance drop could be observed when the stain-scanner combination of training and test data set differed. Multi-institutional data sets improved the average accuracy but did not reach the maximum accuracy of algorithms trained and tested on the same stain-scanner variant (ie, intra-institutional). In summary, DLM-based morphological examination can predict c-KIT-11-ITD with high accuracy in canine MCTs in HE slides. However, staining protocol and scanner type influence accuracy. Larger data sets of scans from different laboratories and scanners may lead to more robust DLMs to identify c-KIT mutations in HE slides.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"152-160"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11874474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reviewers Summary. 审查员总结。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-01 DOI: 10.1177/03009858251314969

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Response to "Letters or not, here we come! A communal perspective on credentials needed for a productive career in veterinary pathology". 对 "不管有没有文凭，我们都来了！兽医病理学领域富有成效的职业生涯所需证书的共同视角"。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-01 Epub Date: 2024-12-18 DOI: 10.1177/03009858241306413

Grant Maxie

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Image challenge in Veterinary Pathology, answers: Alimentary diseases. 兽医病理学中的影像挑战，答案：消化道疾病。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-01 DOI: 10.1177/03009858241300558

引用次数: 0

Insulinoma-associated protein 1 (INSM1) immunohistochemical expression in normal, hyperplastic, and neoplastic canine neuroendocrine tissues. 正常、增生和肿瘤性犬神经内分泌组织中胰岛素瘤相关蛋白 1 (INSM1) 的免疫组化表达。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-01 Epub Date: 2024-09-30 DOI: 10.1177/03009858241279127

Fu-Hua Yang, Ya-Pei Chang, Yen-Chen Chang, Hui-Wen Chang, Chian-Ren Jeng, Jia-Wei Jiang, Wen-Tien Tsao, I-Chi Luo, Wei-Hsiang Huang

{"title":"Insulinoma-associated protein 1 (INSM1) immunohistochemical expression in normal, hyperplastic, and neoplastic canine neuroendocrine tissues.","authors":"Fu-Hua Yang, Ya-Pei Chang, Yen-Chen Chang, Hui-Wen Chang, Chian-Ren Jeng, Jia-Wei Jiang, Wen-Tien Tsao, I-Chi Luo, Wei-Hsiang Huang","doi":"10.1177/03009858241279127","DOIUrl":"10.1177/03009858241279127","url":null,"abstract":"Insulinoma-associated protein 1 (INSM1), a recently identified neuroendocrine marker, is a transcriptional regulator with highly conserved INSM1 homologues in various species. This study investigated the immunohistochemical reactivity of the INSM1 antibody in 20 normal canine neuroendocrine tissues from various anatomical locations, 87 hyperplastic or neoplastic tissues of neuroendocrine origin, and 62 non-neuroendocrine neoplasms and compared the results with those of chromogranin A and synaptophysin in neuroendocrine neoplasms. Western blot was performed on fresh canine pituitary glands and canine parathyroid glands to confirm the specificity of the anti-INSM1 antibody. The results showed that the anti-INSM1 antibody could detect nuclear expression in normal canine neuroendocrine tissues, except for the parathyroid glands. INSM1 was detectable in 79/87 (91%) of the hyperplastic and neoplastic neuroendocrine lesions, but all parathyroid carcinomas and parathyroid adenomas (three samples each) were negative for INSM1. In contrast, INSM1 was detected in only one of 62 (2%) non-neuroendocrine neoplasms. The overall percentage of neuroendocrine neoplasms that immunolabeled positively for all three markers was 89%. In addition, the nuclear expression of INSM1 was easier to interpret than that of chromogranin A or synaptophysin. These findings confirm that INSM1 is a useful immunohistochemical marker for diagnosing canine neuroendocrine neoplasms, except for parathyroid neoplasms, and should be considered as part of immunohistochemistry panels to improve diagnostic capability.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"178-186"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Acute myeloid leukemia with peripheral lymph node involvement in dogs: A retrospective study of 23 cases. 犬急性髓性白血病伴外周淋巴结受累：23 例病例的回顾性研究。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2025-03-01 Epub Date: 2024-11-14 DOI: 10.1177/03009858241295397

Jaspreet Kaur, Gabriella Diamantino, Katherine Morrison, Kristina Meichner, Nora L Springer, Martha Hoffman, Dorothee Bienzle, Tracy Stokol

{"title":"Acute myeloid leukemia with peripheral lymph node involvement in dogs: A retrospective study of 23 cases.","authors":"Jaspreet Kaur, Gabriella Diamantino, Katherine Morrison, Kristina Meichner, Nora L Springer, Martha Hoffman, Dorothee Bienzle, Tracy Stokol","doi":"10.1177/03009858241295397","DOIUrl":"10.1177/03009858241295397","url":null,"abstract":"Acute myeloid leukemia (AML) can infiltrate extramedullary tissues, such as the liver, spleen, and lymph nodes and can be difficult to differentiate from lymphoma in cytologic and histologic specimens. Our goal was to identify cytologic features that would support a diagnosis of AML in peripheral lymph node aspirates, for which we used the term extramedullary AML (eAML). Medical records of 23 dogs with a diagnosis of AML and archived lymph node aspirate smears from 2016 to 2024 were reviewed across 4 institutions. Inclusion criteria included ≥50% myeloid blasts plus differentiating myeloid cells in lymph node smears, confirmation of myeloid lineage by flow cytometric analysis, and complete medical records. Peripheral lymphadenopathy was the reason for presentation (9/23, 39%) or was found incidentally on physical examination (14/23, 61%). Most dogs were bi- or pancytopenic (18/23, 78%), with blasts identified in blood smears of 18 dogs (78%). Initial lymph node aspirate interpretations included hematopoietic neoplasia (8/21, 38%), AML (6/21, 29%), lymphoma (5/21, 24%), lymphoid hyperplasia (1/21, 5%), and granulocytic precursor infiltrates (1/21, 5%). On lymph node smear review, cytologic features supporting an eAML were differentiating granulocytes, blasts with myeloid features or promonocytes, dysplastic changes in myeloid cells, and retention of residual lymphocytes. The median survival was 22 days (range = 1-360 days), and 69% of 16 dogs given chemotherapy or glucocorticoids lived for 30 days or more. Our study highlights the importance of hemogram results and lymph node aspirate smear examination for morphologic features of myeloid differentiation to help diagnose eAML in lymph node smears.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"195-205"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142628884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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