保存时间或固定剂配方对 Porites evermanni 和 Montipora capitata 的组织学没有明显影响。

IF 2.3 2区农林科学 Q2 PATHOLOGY

Veterinary Pathology Pub Date : 2025-01-05 DOI:10.1177/03009858241309403

Thierry M Work, Chutimon Singhakarn, Amy Webb, Norton Chan, Michelle M Dennis

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引用次数: 0

摘要

采集珊瑚进行组织学检查时，需要将样本在海水中保存一段时间，然后将其固定以进行组织学处理。这可能会对组织学检查中形态学变化的解释产生不利影响。我们评估了用原海水或人工海水配制的Z-Fix固定前（0-120分钟）在海水中保存的evermanni Porites和Montipora capitata的微观形态。我们没有看到处理对微观形态的明显影响。然而，在88个统计比较中，在考虑错误发现率后，固定前的保持时间与基底壁黏液程度的显著增加有关。

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Holding time or fixative formulation has no obvious effect on histology of Porites evermanni and Montipora capitata.

Collection of coral for histologic examination requires holding of samples in seawater for a time before they are fixed for histologic processing. This could adversely affect the interpretation of morphologic changes during histologic examinations. We evaluated the microscopic morphology of Porites evermanni and Montipora capitata held (0-120 minutes) in seawater prior to fixation in Z-Fix formulated with raw or artificial seawater. We saw no evident effects of treatments on microscopic morphology. However, among 88 statistical comparisons, and after accounting for false discovery rate, holding time prior to fixation was associated with a significant increase in degree of mucosity of basal body walls.

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来源期刊

Veterinary Pathology 农林科学-病理学

CiteScore

4.70

自引率

8.30%

发文量

审稿时长

2 months

期刊介绍： Veterinary Pathology (VET) is the premier international publication of basic and applied research involving domestic, laboratory, wildlife, marine and zoo animals, and poultry. Bridging the divide between natural and experimental diseases, the journal details the diagnostic investigations of diseases of animals; reports experimental studies on mechanisms of specific processes; provides unique insights into animal models of human disease; and presents studies on environmental and pharmaceutical hazards.