Veterinary Pathology最新文献

Immunohistochemical expression of TLR1, TLR2, TLR4, and TLR9 in the different types of lesions associated with bovine paratuberculosis.

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-25 DOI: 10.1177/03009858241302850

David Zapico, José Espinosa, Miguel Criado, Daniel Gutiérrez, María Del Carmen Ferreras, Julio Benavides, Valentín Pérez, Miguel Fernández

{"title":"Immunohistochemical expression of TLR1, TLR2, TLR4, and TLR9 in the different types of lesions associated with bovine paratuberculosis.","authors":"David Zapico, José Espinosa, Miguel Criado, Daniel Gutiérrez, María Del Carmen Ferreras, Julio Benavides, Valentín Pérez, Miguel Fernández","doi":"10.1177/03009858241302850","DOIUrl":"https://doi.org/10.1177/03009858241302850","url":null,"abstract":"The factors that determine the appearance of the different pathologic forms associated with bovine paratuberculosis are not fully understood, but new research suggests a critical role of innate immunity. Toll-like receptors (TLRs) trigger the recognition of invading pathogens by innate immune cells and the onset of specific immune responses. The aim of this work was to assess, immunohistochemically, the expression of TLR1, TLR2, TLR4, and TLR9 in intestinal samples of 20 cows showing different types of paratuberculous lesions: uninfected controls, focal lesions, paucibacillary, and multibacillary diffuse forms. The majority of labeled cells were morphologically consistent with macrophages. A differential cell count was performed in the intestinal lamina propria, gut-associated lymphoid tissue, and mesenteric lymph node. TLR9 immunolabeling between the different types of lesions was compared using a complete H-score. Focal and diffuse paucibacillary forms contained significantly increased TLR2-expressing macrophages outside of the lesions compared with the controls and diffuse multibacillary forms, and moderate TLR9 immunolabeling within granulomas. In the multibacillary granulomatous lesions, the expression of TLR1 and TLR4 was observed as well as increased TLR9 expression compared with the rest of the groups. Differences in the predominance of one type or another of TLR allows us to elucidate the importance of the innate immune response and its possible role in the development of the different types of paratuberculosis lesions.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858241302850"},"PeriodicalIF":2.3,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesangial cell hypercellularity and iron accumulation in the kidney associated with administration of a sickle hemoglobin modulator in CD-1 mice.

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-23 DOI: 10.1177/03009858241306400

Shambhunath Choudhary, Catherine Picut, Sarah R Vargas, Diana Otis, Timothy M Coskran, David Karanian, Jamie K DaSilva, Christopher Houle, Laurence O Whiteley

{"title":"Mesangial cell hypercellularity and iron accumulation in the kidney associated with administration of a sickle hemoglobin modulator in CD-1 mice.","authors":"Shambhunath Choudhary, Catherine Picut, Sarah R Vargas, Diana Otis, Timothy M Coskran, David Karanian, Jamie K DaSilva, Christopher Houle, Laurence O Whiteley","doi":"10.1177/03009858241306400","DOIUrl":"https://doi.org/10.1177/03009858241306400","url":null,"abstract":"The kidney plays an important role in iron homeostasis and mesangial cells (MCs) are phagocytic cells important for glomerular homeostasis. Sickle hemoglobin (HbS) modulators are promising clinical candidates for treatment of sickle cell disease. Although they prevent disease pathophysiology of HbS polymerization and red blood cell (RBC) sickling by increasing hemoglobin oxygen affinity, higher oxygen affinity can also cause transient tissue hypoxia with compensatory increases in erythropoiesis and subsequent increases in RBC turnover. CD-1 mice treated with an HbS modulator for 2 weeks developed higher RBC mass, increased erythropoiesis, and, by 1 month, deposition of intracellular pigments in renal tubular and parietal epithelium. In addition, in mice treated for 26 weeks, pigment was observed in MCs, which was accompanied by glomerular cell aggregates (MC hypercellularity) and tubulo-interstitial inflammation. The pigment was confirmed by Perl's iron staining and transmission electron microscopy (TEM) to be iron-containing proteins. Glomerular cell aggregates were confirmed to be MCs by TEM, and Ki-67 immunolabeling suggested that MC hypercellularity was due to proliferation. Collectively, these findings, along with iron-containing proteins in livers and spleens, suggested that iron overload secondary to increased RBC turnover led to increased renal iron reabsorption. While both MC hypercellularity and tubulo-interstitial inflammation were thought to be responses to long-term accumulation of iron, the former was considered a homeostatic response to eliminate iron, and maintain glomerular structure and function, while the latter was more consistent with an iron-catalyzed oxidative stress response. To our knowledge, this is the first report of MC hypercellularity in a preclinical toxicity study.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858241306400"},"PeriodicalIF":2.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response to "Letters or not, here we come! A communal perspective on credentials needed for a productive career in veterinary pathology". 对 "不管有没有文凭，我们都来了！兽医病理学领域富有成效的职业生涯所需证书的共同视角"。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-18 DOI: 10.1177/03009858241306413

Grant Maxie

引用次数: 0

Three novel neoplasms in Nancy Ma's owl monkeys (Aotus nancymaae).

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-18 DOI: 10.1177/03009858241300549

Rebecca L Bacon, Carolyn L Hodo, Stephanie J Buchl, Martha E Hensel

引用次数: 0

Neurology and comparative medicine influenced my career in neuropathology. 神经病学和比较医学影响了我的神经病理学生涯。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-18 DOI: 10.1177/03009858241303677

Brian A Summers

引用次数: 0

Response to: Certifying "day one ready" pathologists: are we accomplishing our goals? 回应：认证 "第一天就绪 "的病理学家：我们的目标实现了吗？

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-16 DOI: 10.1177/03009858241303204

Lauri Diehl, Jerry Ritchey, Rachel Reams, Amy Durham, Tony Alves, Kevin Esch, Nicky Parry, Paige Carmichael, Tara Arndt, Alicia Olivier, Ryan Jennings, Shelley Burton

引用次数: 0

Certifying "day one ready" pathologists: Are we accomplishing our goals?

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-16 DOI: 10.1177/03009858241302843

Mark R Ackermann, Dalen W Agnew, Linden E Craig, Taryn A Donovan, Jey W Koehler, Ingeborg M Langohr, Christiane V Löhr, Richard Luong, Emily Meseck, Patricia Pesavento, Brian F Porter, Simon L Priestnall, Daniel R Rissi, Duncan S Russell, Davis Seelig, Mee-Ja M Sula, Charles Wiedmeyer, Bruce H Williams, Andrew D Miller

引用次数: 0

Infarctive encephalopathy in zoo-housed gibbons in North America.

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-12 DOI: 10.1177/03009858241302844

Mei Aoki Schultz, Josephine B Rose, Katherine Olstad, Johnny R Cross, Kali A Holder, Katharine L Hope, Beth Richards, Andrew D Miller

{"title":"Infarctive encephalopathy in zoo-housed gibbons in North America.","authors":"Mei Aoki Schultz, Josephine B Rose, Katherine Olstad, Johnny R Cross, Kali A Holder, Katharine L Hope, Beth Richards, Andrew D Miller","doi":"10.1177/03009858241302844","DOIUrl":"https://doi.org/10.1177/03009858241302844","url":null,"abstract":"Progressive neurologic signs without a known underlying etiology have been observed in managed gibbon populations housed at institutions in North America. In 2018, the Gibbon Species Survival Plan initiated a veterinary survey to evaluate clinical histories among gibbons displaying neurologic signs. The clinical results of this survey as well as the results of a centralized histologic review of brain samples from 5 species of managed gibbons displaying neurologic signs are outlined here. Reported neurologic deficits in gibbons surveyed included tremors (12/12, 100%), ataxia/incoordination (11/12, 92%), fine motor skill deficits (11/12, 92%), weakness (10/12, 83%), and mentation deficits (7/12, 58%). Gross lesions were not identified in the brain. However, in affected animals, histology revealed infarctive lesions in the cerebrocortical (14/15, 93% of animals) and cerebellar (10/15, 67% of animals) gray/white matter. Lesions were absent in the thalamus, mesencephalon, and brainstem. Lesions were typified by areas of parenchymal loss, perivascular foamy macrophage accumulation, and extensive gliosis dominated by gemistocytic astrocytes. In all affected animals, vessels located in the center of the lesions had increased tortuosity with loss of medial distinction, variable medial hyperplasia, and multifocal medial vacuolation that was accentuated by Masson's trichrome staining. Thrombotic lesions were not identified, and amyloid was not identified by Congo red staining. Ionized calcium-binding adaptor molecule 1 (IBA1) immunohistochemistry revealed extensive macrophage infiltration, even in areas in which lesions were not identified on histologic examination. This study reveals a pattern of small-vessel disease among gibbons displaying neurologic signs, and the resulting clinical syndrome is here termed \"gibbon infarctive encephalopathy.\"","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858241302844"},"PeriodicalIF":2.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High mortality associated with avian reoviral hepatitis in young quail (Colinus virginianus).

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-12 DOI: 10.1177/03009858241302842

Panchan Sitthicharoenchai, Jianqiang Zhang, Liying Tian, Judith Stasko, Amro Hashish, Cheng-Shun Hsueh, Yuko Sato, Benjamin Hause, Mohamed El-Gazzar

{"title":"High mortality associated with avian reoviral hepatitis in young quail (Colinus virginianus).","authors":"Panchan Sitthicharoenchai, Jianqiang Zhang, Liying Tian, Judith Stasko, Amro Hashish, Cheng-Shun Hsueh, Yuko Sato, Benjamin Hause, Mohamed El-Gazzar","doi":"10.1177/03009858241302842","DOIUrl":"https://doi.org/10.1177/03009858241302842","url":null,"abstract":"High mortality in bobwhite quail chicks (Colinus virginianus) (35%-85%) was reported from a grower flock in Iowa during July and August of 2022. Two diagnostic submissions of dead, 3-day-old quail chicks were received. Postmortem examination revealed multifocal, pinpoint, pale tan foci in the liver of all birds. Histologic examination revealed moderate to severe, acute, multifocal, random necrotizing hepatitis with multinucleated cells and dystrophic mineralization. Metagenomic sequencing of liver detected orthoreovirus. A high level of avian reovirus (ARV) RNA was identified by real-time reverse transcriptase-PCR. ARV was successfully isolated from liver and lung on the Leghorn male hepatoma cell line. In addition, electron microscopy revealed orthoreovirus viral particles and virus factories in the formalin-fixed livers and viral-infected cell culture. This case highlights ARV as a potential cause of hepatitis in quail chicks and should be considered in the differential diagnosis.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858241302842"},"PeriodicalIF":2.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colocalization of hedgehog arterivirus 1 (HhAV-1) and histologic lesions in the European hedgehog (Erinaceus europaeus) with neurological disease.

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-12-12 DOI: 10.1177/03009858241300553

Fabian Z X Lean, Mark F Stidworthy, Akbar Dastjerdi, Tim Partridge, Stephen Smith, Julie Gough, Alejandro Núñez, Becki Lawson, Katharina Seilern-Macpherson

{"title":"Colocalization of hedgehog arterivirus 1 (HhAV-1) and histologic lesions in the European hedgehog (Erinaceus europaeus) with neurological disease.","authors":"Fabian Z X Lean, Mark F Stidworthy, Akbar Dastjerdi, Tim Partridge, Stephen Smith, Julie Gough, Alejandro Núñez, Becki Lawson, Katharina Seilern-Macpherson","doi":"10.1177/03009858241300553","DOIUrl":"https://doi.org/10.1177/03009858241300553","url":null,"abstract":"The European hedgehog (Erinaceus europaeus) is a protected species of conservation concern in the UK. In recent years, there have been multiple incidents of fatal encephalitis in captive hedgehogs in wildlife rescue centers associated with the molecular detection of a hedgehog arterivirus (HhAV-1). However, it remains unclear whether the virus is the causative agent of the central nervous system (CNS) lesions. In a retrospective investigation using postmortem material from 7 captive hedgehogs with neurological disease, and a single hedgehog with previously identified meningoencephalitis, histologic examination was conducted in tandem with viral RNA in situ hybridization (ISH) to appraise tissue distribution of HhAV-1 and the colocalization with histologic lesions. ISH revealed multicellular tropism of HhAV-1 involving monocyte-macrophage and vascular endothelial cells, with viral RNA detected in multiple organs, likely due to endotheliotropism and viremia. In the CNS, encephalomyelitis was mild whilst viral RNA was abundant and widely distributed, particularly in the microglial population and localized to areas with glial nodules. Splenic lymphoid depletion was generally mild but was moderate to severe in 2 septicemic animals. Brain samples from 13 control hedgehogs, found dead in the wild due to predation/trauma, were also screened for HhAV-1, of which 8 tested positive by real-time reverse transcription polymerase chain reaction (RT-PCR) with a low viral load. No CNS lesions or ISH labeling was observed in 2 of these control hedgehogs that could be examined histologically. Combined, these findings indicate that HhAV-1 infections in captive hedgehogs in English wildlife rescue centers may be associated with histopathologic alterations and clinical neurological disease.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"3009858241300553"},"PeriodicalIF":2.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0