Veterinary Pathology最新文献_第2页

A retrospective study of neuroschistosomiasis in aquatic birds. 水禽神经血吸虫病的回顾性研究。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-03-29 DOI: 10.1177/03009858261428494

Emi Sasaki, Amira Abdu, April L Childress, Daniel B Paulsen, James F X Wellehan, Michael M Garner

{"title":"A retrospective study of neuroschistosomiasis in aquatic birds.","authors":"Emi Sasaki, Amira Abdu, April L Childress, Daniel B Paulsen, James F X Wellehan, Michael M Garner","doi":"10.1177/03009858261428494","DOIUrl":"10.1177/03009858261428494","url":null,"abstract":"Schistosomatidae is a family of trematodes that generally have an indirect life cycle, with snails as intermediate hosts in avian schistosomiasis and aquatic birds as definitive hosts. Although multiple studies report avian schistosomes, there are only a few reports describing gross and histological lesions of neuroschistosomiasis in aquatic birds. Herein, a retrospective study including 12 aquatic birds with neuroschistosomiasis was conducted to further characterize the clinical, gross, and histologic findings and to identify the trematodes. The median age of birds was 12 years, and schistosomes were observed in the brain, with or without granulomatous inflammation. Neuroschistosomiasis was likely related to death in 5 out of 12 birds. Twelve formalin-fixed paraffin-embedded (FFPE) samples containing brain, 1 FFPE sample containing intestine, and 1 fresh brain sample were used for polymerase chain reaction (PCR) and sequencing to identify the schistosomes. Dendritobilharzia pulverulenta was identified from 3 out of 13 FFPE samples and 1 fresh brain sample. Neuroschistosomiasis presumptively associated with Dendritobilharzia sp. infection has been reported in swans and geese; however, the identification was based on the clinical signs, lesions, location within the hosts, and the morphology of adult worms without molecular analysis. To the authors' knowledge, the histologic description and molecular identification of schistosomes that cause neuroschistosomiasis in multiple aquatic bird species other than swans and geese have not been reported.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"910-921"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147575466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic challenge in veterinary pathology: Nephropathy, gastropathy, and pericarditis in feedlot cattle. 兽医病理学中的诊断挑战：饲养场牛的肾病、胃病和心包炎。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-03-25 DOI: 10.1177/03009858261435116

Larissa L de Souza, Thabata C A de Menezes, Maria E M da Silva, Rayane C Pupin, Danilo C Gomes, Ricardo A A Lemos

引用次数: 0

Histological and ultrastructural analysis of regional variations in Negri bodies with inner bodies in naturally infected rabid dogs. 自然感染狂犬病犬内格里体带内体区域变异的组织学和超微结构分析。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-05-07 DOI: 10.1177/03009858261441529

Boonkanit Markbordee, Alpha Grace B Cabic, Nuttipa Iamohbhars, Nozomi Shiwa-Sudo, Kazunori Kimitsuki, Mark Joseph M Espino, Leilanie B Nacion, Daria L Manalo, Satoshi Inoue, Chun-Ho Park

{"title":"Histological and ultrastructural analysis of regional variations in Negri bodies with inner bodies in naturally infected rabid dogs.","authors":"Boonkanit Markbordee, Alpha Grace B Cabic, Nuttipa Iamohbhars, Nozomi Shiwa-Sudo, Kazunori Kimitsuki, Mark Joseph M Espino, Leilanie B Nacion, Daria L Manalo, Satoshi Inoue, Chun-Ho Park","doi":"10.1177/03009858261441529","DOIUrl":"10.1177/03009858261441529","url":null,"abstract":"Negri bodies (NBs) are cytoplasmic inclusions associated with rabies virus infection and are a characteristic histopathologic feature of rabies. Although their morphology has been described in humans and experimental animal models, detailed characterization in naturally infected dogs remains limited. The objective of this study was to characterize the histopathologic, immunophenotypic, and ultrastructural features of NBs in naturally infected dogs from the Philippines. Brain tissues from 24 dogs were examined by routine histology, special stains, immunohistochemistry, and indirect immunofluorescence. Transmission electron microscopy was performed in 6 selected cases. In the cerebrum, hippocampus, and cerebellum, NBs were predominantly composed of abundant matrix material and occasionally contained inner bodies (classic NBs), with variable association with bullet-shaped virions and ribosomes. These inclusions were immunoreactive for rabies virus nucleoprotein and phosphoprotein and stained positively with luxol fast blue. Inner bodies were immunoreactive for glycoprotein and matrix protein and were positive with silver impregnation, Nissl, and Bodian stains. In contrast, small inclusions (lyssa bodies) identified in the thalamus and brainstem consisted mainly of immature viral particles with minimal matrix material. Collectively, these findings indicate region-dependent ultrastructural variation in rabies virus-associated inclusions in naturally infected dogs, while supporting a shared viral origin among the different morphologic forms. This characterization further supports the diagnostic value of these inclusions in veterinary neuropathology.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"893-905"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wischnewsky spots in starvation: A human (and humanitarian) forensic perspective. 维什纽斯基观察饥饿：人类（和人道主义）法医的视角。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-06-18 DOI: 10.1177/03009858261459457

Giuseppe Pulin, Maricla Marrone, Roberto Bellacicco, Elisa Paladini, Fortunato Pititto

引用次数: 0

Effects of fixation and demineralization on immunohistochemical assessment of canine bone marrow. 固定和脱矿对犬骨髓免疫组织化学评价的影响。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-03-11 DOI: 10.1177/03009858261426547

Gabriella M L Diamantino, Janet Beeler-Marfisi, Robert A Foster, Gabrielle Monteith, William Sears, Alice Defarges, Dorothee Bienzle

{"title":"Effects of fixation and demineralization on immunohistochemical assessment of canine bone marrow.","authors":"Gabriella M L Diamantino, Janet Beeler-Marfisi, Robert A Foster, Gabrielle Monteith, William Sears, Alice Defarges, Dorothee Bienzle","doi":"10.1177/03009858261426547","DOIUrl":"10.1177/03009858261426547","url":null,"abstract":"The objective of this study was to evaluate the effect of different fixatives and demineralizers on immunohistochemical (IHC) detection of antigens in bone marrow (BM). Sternal BM samples were collected within 24 hours of death from dogs with spontaneous disease, fixed with acetic acid-zinc-formalin (AZF) or 10% neutral-buffered formalin (NBF) for 24 hours, and decalcified with formic acid, hydrochloric acid, or ethylenediaminetetraacetic acid (EDTA) for 1, 12, or 24 hours, respectively. Immunohistochemical sections for CD3, CD20, CD34, CD204, FLT3, and factor VIII-related antigen (FVIII-rAG) were scored for background, signal intensity, and percent positive cells by 4 independent raters. Some endothelial but not hematopoietic cells were CD34 immunopositive, precluding further assessment. Immunopositive BM cells ranged from 1.3% to 18.3% (CD3), 1.3% to 22.3% (CD20), 0.5% to 22.5% (CD204), and 0% to 17.6% (FLT3). Background scores were similar for all antigens and fixatives except for CD3, for which NBF yielded lower scores than AZF. The signal intensity for all IHC assays was similar for AZF and NBF. The percentage of FLT3- and FVIII-rAG-immunopositive cells was slightly higher in AZF- than NBF-fixed samples. Different demineralizers yielded similar results for all IHC assays except higher background for CD20/EDTA. Signal intensity was higher for CD204 in samples demineralized with EDTA rather than acids. Higher percentages of CD204 and FLT3 immunopositive cells were noted with EDTA relative to acid demineralization. Overall, with the use of stringently standardized pre-analytic and processing protocols, all IHC assays yielded acceptable results, and differences between protocols were considered minor.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"842-852"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147436008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical optimization of a panel of antibodies against amyloid precursor protein for immunodetection of axonal injury in neurologic diseases of sheep. 抗淀粉样前体蛋白抗体免疫检测绵羊神经系统疾病轴突损伤的免疫组化优化

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-03-25 DOI: 10.1177/03009858261433523

Jim Manavis, Glyn Chidlow, Peter Blumbergs, Ian Jerrett, John Finnie

{"title":"Immunohistochemical optimization of a panel of antibodies against amyloid precursor protein for immunodetection of axonal injury in neurologic diseases of sheep.","authors":"Jim Manavis, Glyn Chidlow, Peter Blumbergs, Ian Jerrett, John Finnie","doi":"10.1177/03009858261433523","DOIUrl":"10.1177/03009858261433523","url":null,"abstract":"Amyloid precursor protein (APP) is the most sensitive early immunomarker of axonal injury in the brain. Currently, there are several commercially available antibodies to detect axonal spheroids expressing APP. However, immunolabeling of a given molecule can be variable when antibodies are directed to different epitopes. In this study, we optimized a panel of antibodies (22C11, Y188, NAB228, C1/6.1) for labeling APP-immunopositive axonal spheroids in ovine brains and compared the patterns of injury in brains containing spheroids from neurotoxic (clostridial focal symmetrical encephalomalacia [FSE]) and traumatic brain injury. The pattern of axonal spheroid immunolabeling in FSE necrotic foci and trauma-damaged white matter was similar with all 4 antibodies. Only subtle differences in axonal immunolabeling were found probably due to whether the C- or N-terminal fragment of APP was targeted. However, while background labeling with 3 of the antibodies was minimal, there was robust, diffuse labeling of axons due to myelin immunolabeling with the Y188 antibody, which was determined to be specific. Thus, while these antibodies can detect axonal injury with similar efficacy in sheep brains, caution must be taken with Y188 owing to confounding myelin immunolabeling. This study will hopefully serve as a guide for optimal immunodetection of axonal injury in ovine encephalopathies.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"906-909"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147515152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image challenge in Veterinary Pathology. 兽医病理学中的图像挑战。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-08-31 DOI: 10.1177/03009858261451484

引用次数: 0

Naturally occurring lysosomal storage disease consistent with neuronal ceroid lipofuscinosis in a group of 5 related captive rhesus macaques (Macaca mulatta). 5只相关圈养恒河猴（Macaca mulatta）自然发生的溶酶体贮积病与神经性蜡样脂褐质病一致。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-05-12 DOI: 10.1177/03009858261445995

Katherine Olstad, Gabrielle Pastenkos, Javier Barrera-Zarate, Sreetharan Kanthaswamy, Rebecca Sammak, Marie-Josee Lemoy

{"title":"Naturally occurring lysosomal storage disease consistent with neuronal ceroid lipofuscinosis in a group of 5 related captive rhesus macaques (Macaca mulatta).","authors":"Katherine Olstad, Gabrielle Pastenkos, Javier Barrera-Zarate, Sreetharan Kanthaswamy, Rebecca Sammak, Marie-Josee Lemoy","doi":"10.1177/03009858261445995","DOIUrl":"10.1177/03009858261445995","url":null,"abstract":"Lysosomal storage diseases (LSDs) are a diverse group of inherited disorders characterized by the accumulation of undegraded macromolecules within lysosomes, leading to cellular dysfunction and progressive organ damage. Neuronal ceroid lipofuscinosis (NCL) is a subset of LSDs defined by the accumulation of autofluorescent lipopigments in neurons and, in some cases, peripheral tissues. While naturally occurring LSDs have been documented in domestic and laboratory species, reports in nonhuman primates (NHPs) remain rare. This study describes an LSD in 5 related rhesus macaques (Macaca mulatta) at the California National Primate Research Center, with clinical and histopathologic features supportive of NCL. Affected animals exhibited progressive cognitive and motor deficits, along with an atypical musculoskeletal phenotype including coarse facial features. Histologic examination revealed neuronal cytoplasmic vacuolation, primarily in the cerebellum and brainstem, with storage material exhibiting autofluorescence, positive staining with Sudan black, and luxol fast blue, and a positive periodic acid-Schiff reaction. Transmission electron microscopy identified structures resembling curvilinear inclusions and granular osmiophilic deposits, supporting a NCL diagnosis. This report expands the spectrum of NCL presentations in NHPs, reinforcing their potential as an alternative model for LSD research. The identification of this naturally occurring NCL model in NHPs may enhance our understanding of translational models for therapeutic strategies.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"938-946"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13317747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147875941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wischnewsky spots in starved dogs and cats and their relevance in veterinary forensic pathology. Wischnewsky发现饥饿的狗和猫及其在兽医法医病理学中的相关性。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-05-12 DOI: 10.1177/03009858261445993

Francesca Maccagnan, Ana S Gomes, Hannah McLaughlin, Alyson Blanchard, Sean Taylor, Lorenzo Ressel, Emanuele Ricci

{"title":"Wischnewsky spots in starved dogs and cats and their relevance in veterinary forensic pathology.","authors":"Francesca Maccagnan, Ana S Gomes, Hannah McLaughlin, Alyson Blanchard, Sean Taylor, Lorenzo Ressel, Emanuele Ricci","doi":"10.1177/03009858261445993","DOIUrl":"10.1177/03009858261445993","url":null,"abstract":"In 1895, the German pathologist Dr S.M. Wischnewsky described black, plaque-like discolorations of the gastric mucosa in humans whose cause of death was associated with hypothermia. The pathogenesis of these gastric lesions, known as Wischnewsky spots, is thought to be associated with blood redistribution mechanisms as the body attempts to preserve core body temperature in hypothermic conditions. However, the complete pathogenesis is not fully understood. The relationship between Wischnewsky spots and hypothermia in nonhuman species has also been documented, but again, such lesions in veterinary pathology have an unclear pathogenesis. This study investigated the presence of Wischnewsky spots in several veterinary forensic cases involving 89 emaciated dogs and 46 emaciated cats submitted to the Department of Veterinary Pathology, University of Liverpool, from 2016 to 2023. The gastric lesions were categorized, and collected data were used to compare the characteristics of those lesions found within animals deemed to have died from starvation compared with those having died via cachexic mechanisms. This study aimed to further understand the pathogenesis and diagnostic significance of Wischnewsky spots in veterinary forensic cases. The results indicated that Wischnewsky spots have a significant association with cats and dogs deemed to have died by starvation compared with cases of cachexia. Although comprehensive pathogenesis remains unclear, veterinary forensic cases with Wischnewsky spots are likely to be linked to the combination of stress and hypothermia experienced by cats and dogs that die because of starvation.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"865-870"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147875943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The 2026 ACVP Annual Meeting has world class educational content and award-winning presentations for all facets of pathology. 2026年ACVP年会有世界级的教育内容和获奖的病理各方面的演讲。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2026-09-01 Epub Date: 2026-08-31 DOI: 10.1177/03009858261465449

Katharine Horzmann

引用次数: 0