Veterinary Pathology最新文献_第10页

Diversity of mutations in the dystrophin gene and details of muscular lesions in porcine dystrophinopathies. 猪肌营养不良症中肌营养不良蛋白基因突变的多样性和肌肉病变的细节。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-11-25 DOI: 10.1177/03009858231214028

Yumiko Kamiya, Naoyuki Aihara, Takanori Shiga, Noriyuki Horiuchi, Junichi Kamiie

{"title":"Diversity of mutations in the dystrophin gene and details of muscular lesions in porcine dystrophinopathies.","authors":"Yumiko Kamiya, Naoyuki Aihara, Takanori Shiga, Noriyuki Horiuchi, Junichi Kamiie","doi":"10.1177/03009858231214028","DOIUrl":"10.1177/03009858231214028","url":null,"abstract":"During meat inspections in pigs, dystrophinopathies are among the muscle lesions targeted for disposal. In this study, the authors examined the lesions and the distribution of dystrophin expression in 25 pigs with dystrophinopathy. In addition, complementary deoxyribonucleic acid (cDNA) sequencing and western blotting were performed in 6 of the 25 cases, all of which were characterized by degeneration, necrosis, and fat replacement of muscle fibers. Comparing the results of immunohistochemistry with anti-dystrophin antibodies that recognized at different sites in the protein, the authors noted that the loss of dystrophin expression was most pronounced in the C-terminus-recognizing antibody (19/25 cases). The authors detected 5 missense mutations and 3 types of shortened transcripts generated by the skipping of exons in the cDNA, which were associated with the pathogenesis. One missense mutation had been reported previously, whereas the remaining mutations identified had not been previously documented in pigs. In the cases with shortened transcripts, normal-sized transcripts were detected together with the defective transcripts, suggesting that these mutations were caused by splicing abnormalities. In addition, they were in-frame mutations, all of which have similar pathogeneses of Becker muscular dystrophy in humans. These cases were 6 months of age and exhibited macroscopic discoloration, fatty replacement, and muscle degeneration, suggesting that the effect of these mutations on skeletal muscle was significant.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"432-441"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138435161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PDL1 immunohistochemistry in canine neoplasms: Validation of commercial antibodies, standardization of evaluation, and scoring systems. 犬肿瘤中的PDL1免疫组织化学：商业抗体的验证、评估标准化和评分系统。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-11-03 DOI: 10.1177/03009858231209410

Luisa Vera Muscatello, Francesca Gobbo, Giancarlo Avallone, Micaela Innao, Cinzia Benazzi, Giulia D'Annunzio, Donatella Romaniello, Massimo Orioles, Mattia Lauriola, Giuseppe Sarli

{"title":"PDL1 immunohistochemistry in canine neoplasms: Validation of commercial antibodies, standardization of evaluation, and scoring systems.","authors":"Luisa Vera Muscatello, Francesca Gobbo, Giancarlo Avallone, Micaela Innao, Cinzia Benazzi, Giulia D'Annunzio, Donatella Romaniello, Massimo Orioles, Mattia Lauriola, Giuseppe Sarli","doi":"10.1177/03009858231209410","DOIUrl":"10.1177/03009858231209410","url":null,"abstract":"Immuno-oncology research has brought to light the paradoxical role of immune cells in the induction and elimination of cancer. Programmed cell death protein 1 (PD1), expressed by tumor-infiltrating lymphocytes, and programmed cell death ligand 1 (PDL1), expressed by tumor cells, are immune checkpoint proteins that regulate the antitumor adaptive immune response. This study aimed to validate commercially available PDL1 antibodies in canine tissue and then, applying standardized methods and scoring systems used in human pathology, evaluate PDL1 immunopositivity in different types of canine tumors. To demonstrate cross-reactivity, a monoclonal antibody (22C3) and polyclonal antibody (cod. A1645) were tested by western blot. Cross-reactivity in canine tissue cell extracts was observed for both antibodies; however, the polyclonal antibody (cod. A1645) demonstrated higher signal specificity. Canine tumor histotypes were selected based on the human counterparts known to express PDL1. Immunohistochemistry was performed on 168 tumors with the polyclonal anti-PDL1 antibody. Only membranous labeling was considered positive. PDL1 labeling was detected both in neoplastic and infiltrating immune cells. The following tumors were immunopositive: melanomas (17 of 17; 100%), renal cell carcinomas (4 of 17; 24%), squamous cell carcinomas (3 of 17; 18%), lymphomas (2 of 14; 14%), urothelial carcinomas (2 of 18; 11%), pulmonary carcinomas (2 of 20; 10%), and mammary carcinomas (1 of 31; 3%). Gastric (0 of 10; 0%) and intestinal carcinomas (0 of 24; 0%) were negative. The findings of this study suggest that PDL1 is expressed in some canine tumors, with high prevalence in melanomas.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"393-401"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71427260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PD-L1 mRNA and protein expression in canine mammary carcinomas: Correlation with histopathological grade and molecular markers. 犬乳腺癌中 PD-L1 mRNA 和蛋白的表达：与组织病理学分级和分子标记物的相关性。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2024-01-28 DOI: 10.1177/03009858241226621

Min-Kyung Bae, Yeong-Ung Ko, Byung-Joon Seung, Jung-Hyang Sur, Nong-Hoon Choe

{"title":"PD-L1 mRNA and protein expression in canine mammary carcinomas: Correlation with histopathological grade and molecular markers.","authors":"Min-Kyung Bae, Yeong-Ung Ko, Byung-Joon Seung, Jung-Hyang Sur, Nong-Hoon Choe","doi":"10.1177/03009858241226621","DOIUrl":"10.1177/03009858241226621","url":null,"abstract":"Programmed death ligand 1 (PD-L1) is an immune checkpoint molecule that plays a crucial role in regulating antitumor immune responses. Canine mammary carcinomas (CMCs) are common tumors of dogs. Despite extensive studies on the heterogeneity of CMCs, there is still a lack of effective precision therapies for the treatment of CMCs. In this study, we aimed to investigate the correlation between PD-L1 mRNA and protein expression in CMCs and explore its association with histopathological grade and molecular markers, including the estrogen receptor, epidermal growth factor receptor 2, and cytokeratin 5/6 (CK5/6). Formalin-fixed paraffin-embedded samples were evaluated for PD-L1 mRNA expression using RNA in situ hybridization and PD-L1 protein expression using immunohistochemistry. We observed no substantial correlation between PD-L1 mRNA and protein expression in CMCs; however, PD-L1 mRNA levels were significantly higher in grade 3 than in grade 1 tumors (P = .001). In addition, we observed a positive correlation between PD-L1 protein expression and CK5/6 expression in CMCs (P = .032). These findings suggest that PD-L1 expression in CMCs is heterogeneous and may be regulated post-transcriptionally. Further studies are needed to explore the prognostic and therapeutic implications of PD-L1 expression in different molecular subtypes of CMCs and their potential as predictive biomarkers for immunotherapy.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"402-409"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic challenge in veterinary pathology: Detection of BRAF^V595E mutation in a dog with follicular cystitis and flat urothelial lesion with atypia. 兽医病理学诊断挑战：在一只患有滤泡性膀胱炎和扁平尿道非典型病变的狗体内检测到 BRAFV595E 突变。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-12-13 DOI: 10.1177/03009858231217242

James K Chambers, Naohiro Takahashi, Shizuka Kato, Yuko Hashimoto, Yuko Goto-Koshino, Kazuyuki Uchida

引用次数: 0

Highly pathogenic avian influenza virus H5N1 infection in skua and gulls in the United Kingdom, 2022. 2022 年英国鸥鼬和海鸥感染高致病性禽流感病毒 H5N1。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-12-23 DOI: 10.1177/03009858231217224

Fabian Z X Lean, Marco Falchieri, Natalia Furman, Glen Tyler, Caroline Robinson, Paul Holmes, Scott M Reid, Ashley C Banyard, Ian H Brown, Catherine Man, Alejandro Núñez

{"title":"Highly pathogenic avian influenza virus H5N1 infection in skua and gulls in the United Kingdom, 2022.","authors":"Fabian Z X Lean, Marco Falchieri, Natalia Furman, Glen Tyler, Caroline Robinson, Paul Holmes, Scott M Reid, Ashley C Banyard, Ian H Brown, Catherine Man, Alejandro Núñez","doi":"10.1177/03009858231217224","DOIUrl":"10.1177/03009858231217224","url":null,"abstract":"The reemergence of the highly pathogenic avian influenza virus (HPAIV) subtype H5N1 in the United Kingdom in 2021-2022 has caused unprecedented epizootic events in wild birds and poultry. During the summer of 2022, there was a shift in virus transmission dynamics resulting in increased HPAIV infection in seabirds, and consequently, a profound impact on seabird populations. To understand the pathological impact of HPAIV in seabirds, we evaluated the virus antigen distribution and associated pathological changes in the tissues of great skua (Stercorarius skua, n = 8), long-tailed skua (Stercorarius longicaudus, n = 1), European herring gull (Larus argentatus, n = 5), and black-headed gull (Chroicocephalus ridibundus, n = 4), which succumbed to natural infection of HPAIV during the summer of 2022. Cases were collected from Shetland, including Scatness (mainland), No Ness (mainland), Clumlie (mainland), Hermaness (island), Fair Isle (island), Noss (island), and the West Midlands, South East, and South West of England. Grossly, gizzard ulceration was observed in one great skua and pancreatic necrosis was observed in 4 herring gulls, with intralesional viral antigen detected subsequently. Microscopical analysis revealed neuro-, pneumo-, lymphoid-, and cardiomyotropism of HPAIV H5N1, with the most common virus-associated pathological changes being pancreatic and splenic necrosis. Examination of the reproductive tract of the great skua revealed HPAIV-associated oophoritis and salpingitis, and virus replication within the oviductal epithelium. The emergence of HPAIV in seabirds Stercorariidae and Laridae, particularly during summer 2022, has challenged the dogma of HPAIV dynamics, posing a significant threat to wild bird life with potential implications for the reproductive performance of seabirds of conservation importance.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"421-431"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138886093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subcutaneous choriocarcinomas in captive Amargosa voles (Microtus californicus scirpensis). 圈养Amargosa田鼠的皮下绒毛膜癌。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-10-13 DOI: 10.1177/03009858231203647

Sebastian E Carrasco, Amanda L Johnson, Kerriann M Casey, Nora Allan, Mia Reed, Janet E Foley, Denise M Imai

{"title":"Subcutaneous choriocarcinomas in captive Amargosa voles (Microtus californicus scirpensis).","authors":"Sebastian E Carrasco, Amanda L Johnson, Kerriann M Casey, Nora Allan, Mia Reed, Janet E Foley, Denise M Imai","doi":"10.1177/03009858231203647","DOIUrl":"10.1177/03009858231203647","url":null,"abstract":"Spontaneous choriocarcinomas are rare, highly vascular, malignant trophoblastic tumors that occur in humans and animals. This report describes the unusual spontaneous presentation of 4 choriocarcinomas within the subcutaneous tissues of 4, multiparous but nongravid, Amargosa voles (Microtus californicus scirpensis) from a captive breeding colony. Two subcutaneous neoplasms were composed of multifocal discohesive and infiltrative aggregates of medium to large trophoblasts and cytotrophoblasts within a fibrovascular stroma. Neoplastic cells were associated with variably sized thrombi and cavitary areas of hemorrhage and necrosis. Two subcutaneous tumors were predominantly composed of expansile, blood-filled, cystic spaces lined by neoplastic cytotrophoblasts and occasionally contained medium to large trophoblasts. Trophoblasts and cytotrophoblasts were positive for pancytokeratin and cytokeratin 8/18, negative for alpha-fetoprotein, and contained intracytoplasmic Periodic acid-Schiff (PAS)-positive glycogen in all 4 tumors. In species with hemochorial placentation, migration of trophoblasts into maternal circulation with embolization to distant nonreproductive tissues occurs and may explain the unusual subcutaneous distribution of these 4 tumors. The 2 multiloculated paucicellular tumors may represent an early stage of neoplastic transformation. To the authors' knowledge, this is the first report characterizing choriocarcinomas in extrareproductive sites in rodents.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"476-481"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41214231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melan-A immunolabeling in canine extramedullary plasmacytomas 犬髓外浆细胞瘤中的 Melan-A 免疫标记

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-04-20 DOI: 10.1177/03009858241246979

Lukas Schuwerk, Anastasiia Ulianytska, Wolfgang Baumgärtner, Wencke Reineking

{"title":"Melan-A immunolabeling in canine extramedullary plasmacytomas","authors":"Lukas Schuwerk, Anastasiia Ulianytska, Wolfgang Baumgärtner, Wencke Reineking","doi":"10.1177/03009858241246979","DOIUrl":"https://doi.org/10.1177/03009858241246979","url":null,"abstract":"Histologic diagnosis of less well-differentiated cases of canine extramedullary plasmacytomas (CEMPs) may require immunohistochemical confirmation to discriminate these tumors from other round cells tumors including lymphoma, cutaneous histiocytoma, and amelanotic melanomas. CEMPs are characterized by widespread immunoreactivity for multiple myeloma 1 (MUM1) antigen and λ light chains, while the melanocytic marker melan-A has been reported to yield negative results. Here, 33 randomly selected CEMPs, 20 melanocytomas, and 20 malignant melanomas were immunohistochemically tested for MUM1, melan-A, and PNL2. In addition, CEMPs were examined for PAX5, E-cadherin, CD3, CD18, CD20, S100, as well as λ and κ light chain immunoreactivity. All CEMPs were characterized by labeling for MUM1 and λ light chain, as well as variable immunopositivity for the remaining antibodies. Notably, 13 cases of CEMPs (39.4%) exhibited immunolabeling for melan-A. Melanocytic tumors immunolabeled for melan-A (40/40; 100%) and PNL2 (34/40; 85%). An unexpected cytoplasmic immunoreactivity for MUM1 was observed in 2 melanocytic tumors. Summarized, MUM1 or melan-A immunomarkers alone are not sufficient to differentiate between CEMPs and amelanotic melanomas and should be part of a larger immunopanel including λ light chain, CD20, and PNL2.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":"3 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140630156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue and cellular tropism of Eptesicus fuscus gammaherpesvirus in big brown bats, potential role of pulmonary intravascular macrophages 大棕蝠组织和细胞中的大棕蝠γ疱疹病毒滋养特性，肺血管内巨噬细胞的潜在作用

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-04-15 DOI: 10.1177/03009858241244849

Ursula G. Perdrizet, Janet E. Hill, LaRhonda Sobchishin, Baljit Singh, Champika Fernando, Trent K. Bollinger, Vikram Misra

{"title":"Tissue and cellular tropism of Eptesicus fuscus gammaherpesvirus in big brown bats, potential role of pulmonary intravascular macrophages","authors":"Ursula G. Perdrizet, Janet E. Hill, LaRhonda Sobchishin, Baljit Singh, Champika Fernando, Trent K. Bollinger, Vikram Misra","doi":"10.1177/03009858241244849","DOIUrl":"https://doi.org/10.1177/03009858241244849","url":null,"abstract":"Gammaherpesviruses (γHVs) are recognized as important pathogens in humans but their relationship with other animal hosts, especially wildlife species, is less well characterized. Our objectives were to examine natural Eptesicus fuscus gammaherpesvirus (EfHV) infections in their host, the big brown bat ( Eptesicus fuscus), and determine whether infection is associated with disease. In tissue samples from 132 individual big brown bats, EfHV DNA was detected by polymerase chain reaction in 41 bats. Tissues from 59 of these cases, including 17 from bats with detectable EfHV genomes, were analyzed. An EfHV isolate was obtained from one of the cases, and electron micrographs and whole genome sequencing were used to confirm that this was a unique isolate of EfHV. Although several bats exhibited various lesions, we did not establish EfHV infection as a cause. Latent infection, defined as RNAScope probe binding to viral latency-associated nuclear antigen in the absence of viral envelope glycoprotein probe binding, was found within cells of the lymphoid tissues. These cells also had colocalization of the B-cell probe targeting CD20 mRNA. Probe binding for both latency-associated nuclear antigen and a viral glycoprotein was observed in individual cells dispersed throughout the alveolar capillaries of the lung, which had characteristics of pulmonary intravascular macrophages. Cells with a similar distribution in bat lungs expressed major histocompatibility class II, a marker for antigen presenting cells, and the existence of pulmonary intravascular macrophages in bats was confirmed with transmission electron microscopy. The importance of this cell type in γHVs infections warrants further investigation.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":"36 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140588721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ORF virus causes tumor-promoting inflammation in sheep and goats ORF 病毒导致绵羊和山羊出现肿瘤促进炎症

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-04-13 DOI: 10.1177/03009858241241794

Davide Pintus, Maria G. Cancedda, Giantonella Puggioni, Rosario Scivoli, Angela M. Rocchigiani, Caterina Maestrale, Elisabetta Coradduzza, Roberto Bechere, Luciana Silva-Flannery, Hannah A. Bullock, Simona Macciocu, Maria A. Montesu, Vincenzo Marras, Simone Dore, Jana M. Ritter, Ciriaco Ligios

{"title":"ORF virus causes tumor-promoting inflammation in sheep and goats","authors":"Davide Pintus, Maria G. Cancedda, Giantonella Puggioni, Rosario Scivoli, Angela M. Rocchigiani, Caterina Maestrale, Elisabetta Coradduzza, Roberto Bechere, Luciana Silva-Flannery, Hannah A. Bullock, Simona Macciocu, Maria A. Montesu, Vincenzo Marras, Simone Dore, Jana M. Ritter, Ciriaco Ligios","doi":"10.1177/03009858241241794","DOIUrl":"https://doi.org/10.1177/03009858241241794","url":null,"abstract":"ORF virus (ORFV) causes contagious ecthyma (“ORF”), a disease of sheep and goats characterized by lesions ranging from vesicles and pustules to atypical papilloma-like and angiomatous lesions in the skin and mucosae. The authors investigated the molecular factors leading to the ORF-associated atypical tumor-like changes. Fifteen lambs, 15 kids, and an adult ram clinically affected by natural ORFV infection were enrolled in the study and examined by several methods. ORFV was detected by viral culture or real-time polymerase chain reaction (RT-PCR) in the lesioned tissues and in the blood of the clinically affected sheep and goats. Surprisingly, ORFV was also detected in the blood of healthy goats from an affected herd. Microscopically, they found a pseudo-papillomatous proliferation of the epithelium, while the dermis and lamina propria were expanded by a proliferating neovascular component that highly expressed the viral vascular endothelial growth factor (vVEGF) and its host receptor vascular endothelial growth factor receptor 2 (VEGFR2). Immunohistochemistry, immunofluorescence, and in situ hybridization for mRNA showed that epidermal growth factor receptor (EGFR) was expressed in the fibrovascular component, in the infiltrating CD163+ macrophages, and in the basal stratum of the epidermis. Confocal immunofluorescence microscopy demonstrated that CD163+ macrophages were associated with VEGF and VEGFR2. Finally, they found by quantitative RT-PCR the overexpression of the interleukin-6 and VEGFR2 genes in the lesioned tissues. These findings suggest that ORFV activates an inflammatory reaction characterized by CD163+ macrophages expressing EGFR and VEGFR2, which might play an oncogenic role through synergistic action with vVEGF signaling.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":"26 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140588999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SOX-10 and TRP-1 expression in feline ocular and nonocular melanomas 猫眼部和非眼部黑色素瘤中的 SOX-10 和 TRP-1 表达

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-04-13 DOI: 10.1177/03009858241244850

Margherita Orlandi, Ilaria Porcellato, Monica Sforna, Adriana Lo Giudice, Giuseppe Giglia, Luca Mechelli, Chiara Brachelente

{"title":"SOX-10 and TRP-1 expression in feline ocular and nonocular melanomas","authors":"Margherita Orlandi, Ilaria Porcellato, Monica Sforna, Adriana Lo Giudice, Giuseppe Giglia, Luca Mechelli, Chiara Brachelente","doi":"10.1177/03009858241244850","DOIUrl":"https://doi.org/10.1177/03009858241244850","url":null,"abstract":"In felines, ocular and nonocular melanomas are uncommon tumors that represent a diagnostic challenge for pathologists, especially when amelanotic. To date, the immunohistochemical diagnostic panel in cats is based on specific melanocytic markers (Melan-A and PNL2) and a nonspecific but sensitive marker (S100). In human medicine, SOX-10 is reported to be a sensitive antibody for the detection of melanoma micrometastasis in the lymph node. TRP-1, an enzyme involved in melanogenesis, has recently been used in humans and dogs as a specific melanocyte marker. The aim of this study was to evaluate the cross-reactivity and the expression of SOX-10 and TRP-1 antibodies in feline normal tissue and melanocytic tumors. Thirty-one cases of ocular, cutaneous, and oral melanomas were retrospectively evaluated and confirmed by histopathological examination and by immunolabeling with Melan-A and/or PNL2. SOX-10 nuclear expression in normal tissues was localized in epidermal, subepidermal, hair bulb, and iridal stromal melanocytes and dermal nerves. In melanomas, nuclear expression of SOX-10 was detected in ocular (11/12; 92%), oral (6/7; 86%), and cutaneous sites (12/12; 100%). TRP-1 cytoplasmic immunolabeling in normal tissue was observed in epidermal and bulbar melanocytes and in the lining pigmented epithelium of the iris and in its stroma. Its expression was positively correlated to the degree of pigmentation in the tumor and was observed in 75% of ocular (9/12), 43% of oral (3/7), and 33% of cutaneous melanomas (4/12). This study demonstrated the cross-reactivity of SOX-10 and TRP-1 antibodies in feline non-neoplastic melanocytes and their expression in ocular and nonocular melanomas.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":"68 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140588726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0