Veterinary Pathology最新文献_第9页

Naturally acquired equine parvovirus-hepatitis is associated with a wide range of hepatic lesions in horses. 自然获得的马细小病毒肝炎与马的广泛肝脏病变有关。

IF 2.3 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-11-29 DOI: 10.1177/03009858231214024

Mason C Jager, Eunju Choi, Joy E Tomlinson, Gerlinde Van de Walle

{"title":"Naturally acquired equine parvovirus-hepatitis is associated with a wide range of hepatic lesions in horses.","authors":"Mason C Jager, Eunju Choi, Joy E Tomlinson, Gerlinde Van de Walle","doi":"10.1177/03009858231214024","DOIUrl":"10.1177/03009858231214024","url":null,"abstract":"Equine parvovirus-hepatitis (EqPV-H) is the causative agent of Theiler's disease, or severe acute hepatic necrosis, in horses. However, it is poorly understood whether EqPV-H is associated with other histologic findings in horses with clinical liver disease. The objective of this study was to examine the prevalence and severity of EqPV-H infections in diagnostic liver samples. Archived formalin-fixed, paraffin-embedded (FFPE) liver samples (n = 98) from Cornell University and University of California, Davis, collected between 2007 and 2022 were evaluated for 15 individual histologic features and by EqPV-H in situ hybridization. EqPV-H was detected in 48% (n = 47) of samples. The most common histologic features of EqPV-H-positive samples included individual hepatocyte death (n = 40, 85%), lobular infiltrates (n = 38, 80%), portal infiltrates (n = 35, 74%), and ductular reaction (n = 33, 70%). Centrilobular necrosis, portal infiltrate, and individual hepatocyte death were positively associated with high viral load. Neutrophil infiltrates, bridging fibrosis, and portal edema were negatively associated with a high viral load. Only 4 of 49 tested samples were positive for equine hepacivirus by polymerase chain reaction (PCR), but the PCR assay was unreliable for FFPE tissues. In summary, this study demonstrates that EqPV-H is common in a variety of liver pathologies and should be considered as a differential diagnosis in cases of hepatitis other than Theiler's disease.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"442-452"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11068485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138452602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pro-inflammatory cytokine expression and the STAT1/3 pathway in canine chronic enteropathy and intestinal T-cell lymphoma. 犬慢性肠病和肠道T细胞淋巴瘤中促炎细胞因子的表达和STAT1/3通路。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-10-31 DOI: 10.1177/03009858231207017

Kazuhiro Kojima, James K Chambers, Ko Nakashima, Kazuyuki Uchida

{"title":"Pro-inflammatory cytokine expression and the STAT1/3 pathway in canine chronic enteropathy and intestinal T-cell lymphoma.","authors":"Kazuhiro Kojima, James K Chambers, Ko Nakashima, Kazuyuki Uchida","doi":"10.1177/03009858231207017","DOIUrl":"10.1177/03009858231207017","url":null,"abstract":"The accumulation of intraepithelial lymphocytes (IELs) is a histopathological feature of canine chronic enteropathy (CE), and IELs are considered the cells of origin of intestinal T-cell lymphoma (ITCL). However, the pathogenic mechanism of IEL activation in CE remains unclear. This study hypothesized that the expression of proinflammatory cytokines, associated with cytotoxic T/NK-cell activation, is upregulated in CE and ITCL, and examined the expression of IFN-γ, IL-2, IL-12p35, IL-12p40, IL-15, and IL-21 and the downstream signal transducers and activators of transcription (STAT) pathway in the duodenal mucosa of dogs without lesions (n = 11; NC), with IEL-CE (n = 19; CE without intraepithelial lymphocytosis), IEL+CE (n = 29; CE with intraepithelial lymphocytosis), and with ITCL (n = 60). Quantitative polymerase chain reaction (PCR) revealed that IFN-γ and IL-21 were higher in IEL+CE than in IEL-CE or NC. Western blot revealed upregulation of STAT1 and STAT3 in IEL+CE. Double-labeling immunohistochemistry revealed a positive correlation between the Ki67 index of CD3+ T-cells and IFN-γ expression levels. Immunohistochemistry revealed a higher ratio of p-STAT1-positive villi in IEL+CE and ITCL than IEL-CE and NC, which positively correlated with IFN-γ expression levels. Among the 60 ITCL cases, neoplastic lymphocytes were immunopositive for p-STAT1 in 28 cases and p-STAT3 in 29 cases. These results suggest that IFN-γ and IL-21 contribute to the pathogenesis of IEL+CE, and IFN-γ may be involved in T-cell activation and mucosal injury in CE. STAT1 and STAT3 activation in ITCL cells suggests a role for the upregulation of the STAT pathway in the pathogenesis of ITCL.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"382-392"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71427261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vulvo-vaginal epithelial tumors in mares: A preliminary investigation on epithelial-mesenchymal transition and tumor-immune microenvironment. 母马外阴阴道上皮肿瘤：上皮-间质转化和肿瘤免疫微环境的初步研究。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-11-01 DOI: 10.1177/03009858231207025

Federico Armando, Ilaria Porcellato, Livia de Paolis, Samanta Mecocci, Benedetta Passeri, Małgorzata Ciurkiewicz, Luca Mechelli, Chiara Grazia De Ciucis, Marzia Pezzolato, Floriana Fruscione, Chiara Brachelente, Vittoria Montemurro, Katia Cappelli, Christina Puff, Wolfgang Baumgärtner, Alessandro Ghelardi, Elisabetta Razzuoli

{"title":"Vulvo-vaginal epithelial tumors in mares: A preliminary investigation on epithelial-mesenchymal transition and tumor-immune microenvironment.","authors":"Federico Armando, Ilaria Porcellato, Livia de Paolis, Samanta Mecocci, Benedetta Passeri, Małgorzata Ciurkiewicz, Luca Mechelli, Chiara Grazia De Ciucis, Marzia Pezzolato, Floriana Fruscione, Chiara Brachelente, Vittoria Montemurro, Katia Cappelli, Christina Puff, Wolfgang Baumgärtner, Alessandro Ghelardi, Elisabetta Razzuoli","doi":"10.1177/03009858231207025","DOIUrl":"10.1177/03009858231207025","url":null,"abstract":"Vulvo-vaginal epithelial tumors are uncommon in mares, and data on the epithelial-to-mesenchymal transition (EMT) and the tumor-immune microenvironment (TIME) are still lacking. This is a study investigating the equus caballus papillomavirus type 2 (EcPV2) infection state as well as the EMT process and the tumor microenvironment in vulvo-vaginal preneoplastic/ benign (8/22) or malignant (14/22) epithelial lesions in mares. To do this, histopathological, immunohistochemical, transcriptomic, in situ hybridization, and correlation analyses were carried out. Immunohistochemistry quantification showed that cytoplasmic E-cadherin and β-catenin expression as well as nuclear β-catenin expression were features of malignant lesions, while benign/preneoplastic lesions were mainly characterized by membranous E-cadherin and β-catenin expression. Despite this, there were no differences between benign and malignant equine vulvo-vaginal lesions in the expression of downstream genes involved in the canonical and noncanonical wnt/β-catenin pathways. In addition, malignant lesions were characterized by a lower number of cells with cytoplasmic cytokeratin expression as well as a slightly higher cytoplasmic vimentin immunolabeling. The TIME of malignant lesions was characterized by more numerous CD204+ M2-polarized macrophages. Altogether, our results support the hypothesis that some actors in TIME such as CD204+ M2-polarized macrophages may favor the EMT process in equine vulvo-vaginal malignant lesions providing new insights for future investigations in the field of equine EcPV2-induced genital neoplastic lesions.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"366-381"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71427262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equine sarcoids: A clinicopathologic study of 49 cases, with mitotic count and clinical type predictive of recurrence. 马肉瘤：49例临床病理研究，有丝分裂计数和临床类型可预测复发。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-11-08 DOI: 10.1177/03009858231209408

Wilson Karalus, Supatsak Subharat, Geoff Orbell, Bernie Vaatstra, John S Munday

{"title":"Equine sarcoids: A clinicopathologic study of 49 cases, with mitotic count and clinical type predictive of recurrence.","authors":"Wilson Karalus, Supatsak Subharat, Geoff Orbell, Bernie Vaatstra, John S Munday","doi":"10.1177/03009858231209408","DOIUrl":"10.1177/03009858231209408","url":null,"abstract":"Sarcoids are common mesenchymal neoplasms of horses. Although there are few studies in which sarcoids have been followed over a long period of time, sarcoids are considered locally invasive and have been reported to frequently recur following surgical excision. Currently, no histological features have been identified to predict which sarcoids will recur after excision. The present study comprised 49 sarcoids for which histology sections were available and in which the recurrence status of the case was known. Each sarcoid was excised from a different horse. Overall, 12 of the 49 (24%) sarcoids recurred after surgical excision. Mitotic count (MC), cellularity, necrosis, nuclear pleomorphism, and inflammation of the sarcoids were evaluated histologically. Of these, MC correlated with recurrence. Four of 5 (80%) sarcoids with an MC ≥ 20 in 2.37 mm2 recurred, which was a significantly higher recurrence rate than that of sarcoids with an MC < 20, 8 of 44 cases recurred (18%), P = .0051. Clinical type was also found to correlate with recurrence. Three of 4 (75%) fibroblastic types recurred, which was a significantly higher recurrence rate than that of sarcoids with other clinical types, 9 of 45 cases (18%), P < .001. In addition, univariate Cox regression analysis confirmed fibroblastic type and MC ≥ 20 as significant predictors for recurrence (P = .016 and P = .005, respectively). To the authors' knowledge, this is the first large study examining recurrence rates in sarcoids, and the first time that histological features have been correlated with recurrence.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"357-365"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11067406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71486617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new genotype of hepatitis A virus causing transient liver enzyme elevations in Mauritius-origin laboratory-housed Macaca fascicularis. 一种新的甲型肝炎病毒基因型引起毛里毛斯实验室饲养的猕猴短暂性肝酶升高。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-11-12 DOI: 10.1177/03009858231209691

Lars Mecklenburg, Rebecca Ducore, Molly Boyle, Andrew Newell, Laura Boone, Joerg Luft, Annette Romeike, Ann-Kathrin Haverkamp, Keith Mansfield, Kelley A Penraat, J J Baczenas, Nick Minor, Shelby L O'Connor, David H O'Connor

{"title":"A new genotype of hepatitis A virus causing transient liver enzyme elevations in Mauritius-origin laboratory-housed Macaca fascicularis.","authors":"Lars Mecklenburg, Rebecca Ducore, Molly Boyle, Andrew Newell, Laura Boone, Joerg Luft, Annette Romeike, Ann-Kathrin Haverkamp, Keith Mansfield, Kelley A Penraat, J J Baczenas, Nick Minor, Shelby L O'Connor, David H O'Connor","doi":"10.1177/03009858231209691","DOIUrl":"10.1177/03009858231209691","url":null,"abstract":"Hepatitis A virus (HAV) infects humans and nonhuman primates, typically causing an acute self-limited illness. Three HAV genotypes have been described so far for humans, and three genotypes have been described for nonhuman primates. We observed transiently elevated liver enzymes in Mauritius-origin laboratory-housed macaques in Germany and were not able to demonstrate an etiology including HAV by serology and polymerase chain reaction (PCR). HAV is a rare pathogen in cynomolgus macaques, and since all employees were routinely vaccinated against HAV, it was not a part of the routine vaccination and screening program. A deep sequencing approach identified a new HAV genotype (referred to as Simian_HAV_Macaca/Germany/Mue-1/2022) in blood samples from affected animals. This HAV was demonstrated by reverse transcription PCR in blood and liver and by in situ hybridization in liver, gall bladder, and septal ducts. A commercial vaccine was used to protect animals from liver enzyme elevation. The newly identified simian HAV genotype demonstrates 80% nucleotide sequence identity to other simian and human HAV genotypes. There was deeper divergence between Simian_HAV_Macaca/Germany/Mue-1/2022 and other previously described HAVs, including both human and simian viruses. In situ hybridization indicated persistence in the biliary epithelium up to 3 months after liver enzymes were elevated. Vaccination using a commercial vaccine against human HAV prevented reoccurrence of liver enzyme elevations. Because available assays for HAV did not detect this new HAV genotype, knowledge of its existence may ameliorate potential significant epidemiological and research implications in laboratories globally.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"488-496"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89719723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review: The PI3K-AKT-mTOR signal transduction pathway in canine cancer. 综述：PI3K-AKT-mTOR信号转导通路在犬癌症中的作用。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-10-31 DOI: 10.1177/03009858231207021

Travis K Meuten, Gregg A Dean, Douglas H Thamm

引用次数: 0

Aneurysm Associated with Vascular Wall Degeneration in Bearded Dragons (Pogona vitticeps). 大胡子龙(Pogona vitticeps)与血管壁变性相关的动脉瘤。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-11-25 DOI: 10.1177/03009858231214025

Joaquín Ortega, Jeanette Wyneken, Michael M Garner

{"title":"Aneurysm Associated with Vascular Wall Degeneration in Bearded Dragons (Pogona vitticeps).","authors":"Joaquín Ortega, Jeanette Wyneken, Michael M Garner","doi":"10.1177/03009858231214025","DOIUrl":"10.1177/03009858231214025","url":null,"abstract":"This study describes the clinical, gross, and histologic findings in 17 cases of aneurysms in bearded dragons (Pogona vitticeps). The clinical presentation ranged from incidental to sudden and unexpected death. The affected vasculature was predominantly arterial; however, based on the topographical locations of the lesions, gross structure, and drainage, some veins were likely involved. Magnetic resonance imaging and computerized tomography scans of 1 animal showed a large aneurysm of the internal carotid artery extending from near its aortic origin into the caudal head. Aneurysms were organized in 5 groups based on their anatomical locations: cephalic, cranial coelom (for all near the heart), caudal coelom (for the mesenteric vessels and descending aorta), limbs, and tail. The cranial coelomic region was the most prevalent location. Gross findings were large hematomas or red serosanguineous fluid filling the adjacent area, as most of the aneurysms (94%) were ruptured at the time of the study. The main histological findings were degenerative changes of the vessel walls characterized by moderate to severe disruption of the collagen and elastic fibers of the tunica media and adventitia (100%), followed by thickening of the intima with thrombi formation (54%) and dissecting hematoma of the vessel wall (47%). Vasculitis (29%), mineralization (6%), and lipid deposits (6%) in the vessel wall were observed occasionally. Based on these findings, the vascular dilations and ruptures observed in bearded dragons likely are associated with weakness of the vessel walls caused by degenerative changes in the intimal and medial tunics.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"468-475"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138435160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neoplasia in an assurance population of Puerto Rican crested toads (Peltophryne lemur). 波多黎各凤头蟾蜍(狐猴)种群的肿瘤。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-11-28 DOI: 10.1177/03009858231214029

Sierra M Imanse, Caitlin E Burrell, Sarah A Cannizzo, Tara S Reilly, Kimberly L Rainwater, Martha A Delaney

引用次数: 0

Exudative glomerulonephritis associated with acute leptospirosis in dogs. 犬急性钩端螺旋体病并发渗出性肾小球肾炎。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-10-29 DOI: 10.1177/03009858231207020

Monika Hilbe, Horst Posthaus, Giulia Paternoster, Simone Schuller, Michelle Imlau, Hanne Jahns

{"title":"Exudative glomerulonephritis associated with acute leptospirosis in dogs.","authors":"Monika Hilbe, Horst Posthaus, Giulia Paternoster, Simone Schuller, Michelle Imlau, Hanne Jahns","doi":"10.1177/03009858231207020","DOIUrl":"10.1177/03009858231207020","url":null,"abstract":"In the past 20 years in Switzerland, dogs with suspect acute leptospirosis frequently showed severe glomerular changes that had not been previously reported. These features were characterized by abundant extravasated erythrocytes and fewer neutrophils accompanied by marked fibrin exudation into the urinary space that was interpreted as an exudative glomerulonephritis (GN). This retrospective study describes this significant glomerular pathological change and investigates the association with leptospirosis. Tissues from 50 dogs with exudative GN, retrieved from 2 pathology archives in Switzerland were reviewed using hematoxylin and eosin, periodic acid-Schiff, phosphotungstic acid-hematoxylin, and Warthin and Starry stains. Clinical and postmortem data were collected for each case. Immunohistochemistry (IHC) and/or polymerase chain reactions were used as confirmatory tests for leptospirosis. While all 50 cases had clinical and pathological features supporting a diagnosis of leptospirosis, 37 cases were confirmed for the disease. Using a LipL32 antibody in addition to the OMV2177 antibody raised against the lipopolysaccharide of Leptospira interrogans serovar Copenhageni increased the detection rate of Leptospira by IHC in exudative GN from 24% to 62%. Signalment, seasonality, clinical signs, blood results, and pathological changes in dogs with exudative GN were similar to those reported for dogs without GN and confirmed infection by Leptospira spp.. Exudative GN was common among Swiss dogs with leptospirosis where it caused acute severe disease. Leptospirosis should be considered as a cause of this new pathologic feature by the pathologist. The pathogenesis remains unclear, but involvement of a geographic-specific serovar with unique virulence factors is suspected and warrants further investigation.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"453-461"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11067394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71413988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myosin heavy-chain myopathy in 2 American quarter horses. 2匹美国四分之一马肌球蛋白重链肌病。

IF 2.4 2区农林科学

Veterinary Pathology Pub Date : 2024-05-01 Epub Date: 2023-10-11 DOI: 10.1177/03009858231204253

Mayane Faccin, Kirsten A Landsgaard, Sarai M Milliron, Alexis H Jennings, M Keith Chaffin, Paula R Giaretta, Raquel R Rech

引用次数: 0