Veterinary Pathology最新文献

{"title":"Cardiac lesions in sheep and goats with spontaneous <i>Clostridium perfringens</i> type D enterotoxemia.","authors":"Emma H Torii, Omar A Gonzales-Viera, Jennine Ochoa, Todd Cornish, Nicolas Streitenberger, Roberto W I Olivares, Hannah Neer, Francisco A Uzal, Asli Mete","doi":"10.1177/03009858261426544","DOIUrl":"10.1177/03009858261426544","url":null,"abstract":"<p><p>A 12-year retrospective search identified 106 sheep and goats diagnosed with <i>Clostridium perfringens</i> type D enterotoxemia, and in which the heart was histologically examined. Twenty cases (20/106, 19%), including 10 sheep and 10 goats, had cardiac lesions that were presumed to be associated with enterotoxemia. The lesions included myocardial degeneration and/or necrosis (<i>n</i> = 16, 80%), hemorrhage (<i>n</i> = 17, 85%), and proteinaceous interstitial edema (<i>n</i> = 6, 30%). Myocardial degeneration and/or necrosis was more frequent in goats (10/10, 100%) compared with sheep (6/10, 60%). Hemorrhage was more frequent in sheep (10/10, 100%) compared with goats (7/10, 70%). Myocardial proteinaceous interstitial edema was exclusive to sheep. Cardiac lesions occur in spontaneous cases of <i>C. perfringens</i> type D enterotoxemia in small ruminants and may play a role in the clinical signs and/or the demise of the animal.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"871-878"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147370123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histologic and immunohistochemical characterization and frequency of rare subtypes of canine invasive urothelial carcinoma.","authors":"Megan P Corbett, Mohamed Elbadawy, Andrew P Woodward, John C Cheville, Hannah Wickham, Hannah F Nicholson, Michael Catucci, Bryan J Melvin, Basant Ahmed, Christopher Zdyrski, Lilian J Oliveira, Elizabeth W Howerth, Aleksandra Pawlak, Olufemi Fasina, Jonathan P Mochel, Karin Allenspach","doi":"10.1177/03009858261428496","DOIUrl":"10.1177/03009858261428496","url":null,"abstract":"<p><p>While most cases of human muscle-invasive bladder cancer (MIBC) are diagnosed as conventional urothelial carcinoma (UC), there is growing recognition of histologic subtypes and divergent differentiation within conventional UC. In this study, 31 dogs with UC (33 slides) were evaluated for histologic subtypes and divergent differentiation, as specified by the recent World Health Organization update on the classification of urinary bladder cancers. Slides were reviewed by a medical uropathologist and 3 board-certified veterinary pathologists and assessed for expression of uroplakin III, vimentin, and E-cadherin. All tumors were classified as UC. Fifteen cases were identified as conventional UC. Among the remainder, 8 displayed glandular differentiation, 4 were classified as sarcomatoid UC, 2 showed squamous differentiation, 1 case was classified as a large nested subtype, and 1 case was classified as a tubular/microcystic subtype. This study found a higher frequency of certain histologic subtypes and divergent differentiation in canine UC, particularly sarcomatoid UC and UC with glandular differentiation, compared to previous reports of both canine UC and human MIBC. The relatively high prevalence of the sarcomatoid UC and UC with glandular differentiation in dogs observed in this study suggests that canine UC may serve as a valuable translational model for evaluating novel therapeutic agents, particularly for these rare and aggressive subtypes in humans.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"807-819"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147515067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphologic and immunohistochemical characteristics of intraocular metastatic carcinomas of presumed pulmonary origin in cats: A retrospective case series from 2019 to 2025.","authors":"Hannah Stocklein, Ryan P Taylor, Gillian C Shaw, Lorelei L Clarke","doi":"10.1177/03009858261445996","DOIUrl":"10.1177/03009858261445996","url":null,"abstract":"<p><p>Carcinomas have been reported to metastasize to the globe, specifically the uvea; however, the distinguishing histologic features of intraocular metastatic carcinomas in cats are not well-documented. Eleven cases of feline ocular biopsies with intraocular metastatic carcinomas and history of a pulmonary mass submitted to the Comparative Ocular Pathology Laboratory of Wisconsin from 2019 to 2025 were collected and reviewed. Alcian blue stains and immunohistochemical markers for thyroid transcription factor-1 and vimentin were evaluated. No significant sex or breed predispositions were identified. Uveitis, glaucoma, and the presence of an ocular mass were the most common clinical reasons for enucleation. All cases had a thoracic mass identified by radiographs prior to enucleation. All neoplasms had carpeting to gland-forming growth patterns lining surfaces of intraocular structures, affecting the anterior uvea. Goblet cells were highlighted with Alcian blue staining in 7/11 cases (64%). Neoplastic cells immunolabeled for thyroid transcription factor-1 in 2/11 cases (18%) (neither of which had goblet cells) and vimentin in 4/11 cases (36%). Based on this study, feline intraocular metastatic neoplasms are commonly associated with glaucoma and less commonly with uveitis. Histomorphologic and immunohistochemical features of pulmonary origin neoplasia were not consistently found in these metastatic masses of suspected pulmonary origin; the presence of goblet cells was the most commonly identified histologic feature.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"820-824"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147875936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydranencephaly and porencephaly predominated among neuropathologic findings in stillborn and neonatal piglets during the first major outbreak of Japanese encephalitis in Australia.","authors":"Willy W Suen, Anne Jordan, Leah K Manning, Selina Ossedryver, Shirley A Turner, Graeme Knowles, Effie Lee, Zoe Spiers, Angel L Ngo, Mark Hazelton, Deborah Finlaison, Peter Kirkland, Cathy Shilton, Jean Payne, Jennifer Harper, Kate Maynard, Yangchen Gurung, Teresa Eastwood, Tristan Reid, David T Williams, Richard Ploeg, John Bingham, Brian A Summers, Pedro Pinczowski","doi":"10.1177/03009858261423130","DOIUrl":"10.1177/03009858261423130","url":null,"abstract":"<p><p>In 2022, piggeries in southeastern Australia experienced an increase in reproductive losses and occasional neurologic disease in neonates with up to 100% mortality in some litters. Molecular testing identified a genotype IV Japanese encephalitis virus (JEV) as the etiological agent, detected for the first time in Australia. Necropsy of 235 JEV-positive domestic piglets, mostly stillbirths with 30% mummified, revealed hydranencephaly-porencephaly impacting the dorsal cerebrum as the most common lesion. Microscopically, this was characterized by varying degrees of parenchymal collapse as a result of liquefactive necrosis, hypoplasia, and dysplasia, with nonsuppurative inflammation and mineralization. In the most severe cases, hydranencephaly reduced the cerebral parenchyma to a thin membrane enclosing dilated lateral ventricles. Histologically, the affected neuroparenchyma was largely devoid of mature neurons, axons, myelin, and oligodendrocytes. What remained was mostly a dense population of IBA-1-positive histiocytes and few CD3-positive T-lymphocytes, interspersed within a dense network of GFAP-positive astrocytic processes. Immunohistochemistry for flaviviral antigen showed colocalization of viral antigen-positive neurons and necrotic foci. In less severe cases, cerebral necrosis occurred in well-demarcated, locally extensive regions or isolated gyri, sometimes with a vascular distribution, resulting in porencephaly. In addition, vascular calcification was noted, although with unknown significance and pathogenesis. Arthrogryposis, scoliosis, and kyphosis were observed in a small subset of the cases, which had myelodysplasia. Myelodysplastic changes varied and included central canal duplication, dysplastic gray matter, and bifurcation of the ventral medial sulcus. Multifocal necrosis and inflammation were also observed in the spinal cord, although the extent was typically limited.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"879-892"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147285234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophilic cholangitis associated with intracholangiocytic <i>Hammondia</i> protozoa confirmed in 2 dogs and suspected in 2 dogs.","authors":"Eunju April Choi, Devinn M Sinnott, Amira A Ahmed, Anibal G Armien, Karen Shapiro, Manigandan L Virapin","doi":"10.1177/03009858261449082","DOIUrl":"10.1177/03009858261449082","url":null,"abstract":"<p><p>Four dogs with neutrophilic cholangitis were identified with intracholangiocytic apicomplexan protozoa. Two cases were biopsies, and both cases had gallbladder involvement. The other two cases were unexpected findings on autopsy. Immunohistochemistry against <i>Toxoplasma gondii</i> on all cases labeled the zoites. <i>Internal transcribed spacer-1</i> polymerase chain reaction (PCR) performed on formalin-fixed paraffin-embedded samples was successful in the 2 biopsy cases, and subsequent sequencing identified >99% sequence identity to <i>Hammondia</i> sp. Of the biopsied dogs, 1 dog recovered successfully after antiprotozoal treatment while the other dog was euthanized due to poor response. The 2 autopsy cases were given immunosuppressive medication. <i>Hammondia</i> are within the Sarcocystidae family of apicomplexan parasites and are closely related to <i>Neospora caninum</i>. This study and the current literature support that <i>Hammondia</i>, specifically <i>Hammondia heydorni</i>, an organism that was previously considered nonpathogenic or minimally pathogenic, can rarely exhibit tropism to the biliary system and cause neutrophilic cholangitis, cholangiohepatitis, and cholecystitis in dogs.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"837-841"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147869629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive phenotypic and clonality analysis of intraepithelial lymphocytes of intestinal T-cell lymphoma and chronic enteropathy in dogs.","authors":"Kazuhiro Kojima, James K Chambers, Ko Nakashima, Yuko Goto-Koshino, Kazuyuki Uchida","doi":"10.1177/03009858261428495","DOIUrl":"10.1177/03009858261428495","url":null,"abstract":"<p><p>Canine intestinal T-cell lymphoma (ITCL) may arise from intraepithelial lymphocyte (IEL) subsets expanded in chronic enteropathy (CE). To investigate this potential cell of origin, we performed flow cytometry, immunohistochemistry, and RNA <i>in situ</i> hybridization on IELs using biopsy samples from 62 dogs, including 6 large-cell lymphomas (LCLs), 9 small-cell lymphomas (SCLs), 31 CEs with increased IELs (IEL⁺CE), and 16 CEs without increased IELs (IEL^-CE). IELs in ITCLs were predominantly CD4^-CD8α^- (LCL, 5/6; SCL, 4/9), and 4/31 IEL⁺CEs had a higher proportion of CD4^-CD8α^- IELs than the other CE cases. Forward scatter values of CD4^-CD8α^- IELs were higher in IEL⁺CEs compared with IEL^-CEs, correlating positively with the Ki-67 index, indicating proliferation of this population. An increase in IELs lacking T-cell receptor expression was observed in 1/4 LCL, 2/5 SCL, and 1/13 IEL⁺CE cases. Lack of surface CD3 was observed in 2/9 SCL cases. One of 5 LCL cases partially expressed NKp46, whereas the proportion of NKp46⁺ IELs was low (< 4%) in 32/33 CE cases examined. In addition, 3/6 LCL cases expressed <i>NKp46</i> mRNA without detectable protein expression. Clonality analysis of isolated IELs yielded at least 1 clonal peak in 1/2 LCLs, 4/7 SCLs, 12/23 IEL⁺CEs, and 4/13 IEL^-CEs, underscoring the limited diagnostic utility on distinguishing ITCL, particularly SCL, from IEL⁺CE. These findings suggest that most canine ITCLs have a CD4^-CD8α^- immunophenotype and that unconventional IELs in IEL⁺CE may be the cells of origin. Moreover, the distinction between ITCL and CE remains challenging, emphasizing the importance of integrated diagnostic approaches.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"853-864"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147582125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does the term nonsuppurative qualify as a morphologic descriptor?","authors":"Daniel R Rissi, M Grant Maxie, Robert A Foster, Claudio S L Barros","doi":"10.1177/03009858261451488","DOIUrl":"10.1177/03009858261451488","url":null,"abstract":"","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"966-967"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147983522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Image challenge in <i>Veterinary Pathology</i>, answers: Skin diseases.","authors":"","doi":"10.1177/03009858261451482","DOIUrl":"https://doi.org/10.1177/03009858261451482","url":null,"abstract":"","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":"63 5","pages":"970-972"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathology and diagnosis of brucellosis (<i>Brucella</i> spp.) in veterinary species.","authors":"Agustín Rebollada-Merino, Lucas Domínguez, Antonio Rodríguez-Bertos","doi":"10.1177/03009858261435106","DOIUrl":"10.1177/03009858261435106","url":null,"abstract":"<p><p>Brucellosis is an infectious disease that affects a wide range of animals, caused by bacteria belonging to 13 species and several biovars within the <i>Brucella</i> genus (<i>B. melitensis</i>, <i>B. abortus</i>, <i>B. suis</i>, <i>B. ovis</i>, <i>B. neotomae</i>, <i>B. canis</i>, <i>B. ceti</i>, <i>B. pinnipedialis</i>, <i>B. microti</i>, <i>B. inopinata</i>, <i>B. papionis</i>, <i>B. vulpis</i>, <i>B. nosferati</i>, and unclassified strains). <i>Brucella</i> spp. infects small ruminants, cattle, swine, dogs, wild mammals, reptiles, amphibians, and fish, and most <i>Brucella</i> spp. have zoonotic potential. Characterization of lesions in animal brucellosis remains paramount in the diagnosis in endemic regions and even for countries free of brucellosis, where passive surveillance plays an indispensable role in upholding and sustaining their status. Pathology also contributes to understanding the pathogenesis and associating emerging pathogenic <i>Brucella</i> spp. with disease in animals. Here, we review the pathology of brucellosis in animals with an emphasis on cross-species transmission of \"classical\" <i>Brucella</i> spp. to nonpreferential hosts and \"atypical\" <i>Brucella</i> spp. We conclude that <i>Brucella</i> is an expansive genus identified in numerous animals, but there are still significant gaps in the knowledge of the pathology of brucellosis in unusual hosts and emerging <i>Brucella</i> spp. This is a great opportunity for veterinary pathologists to spearhead advancements in the knowledge of the pathogenesis and diagnosis of <i>Brucella</i> spp. infections. The isolation of a <i>Brucella</i> spp. and the correlation with lesions should follow molecular diagnostics to define the genetic signature of the isolate, providing a better understanding of epidemiology and contributing to the control of brucellosis across animal and human populations.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"787-806"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147646676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of a metabolic dysfunction-associated steatotic liver disease model in the male Korean field mouse (<i>Apodemus peninsulae</i>): A comparison with the male C57BL/6J mouse.","authors":"Qing Zhang, Xiwen Zhang, Jialei He, Yanzun Li, Shiqi Qiao, Yan Gao, Jinping Hu, Yujing Feng, Bao Yuan, Jian Chen, Wenzhi Ren, Zhe Zhang, Yu Ding","doi":"10.1177/03009858261426545","DOIUrl":"10.1177/03009858261426545","url":null,"abstract":"<p><p>Metabolic dysfunction-associated steatotic liver disease (MASLD) is an increasingly serious global health issue. The establishment of accurate animal models is crucial for elucidating its pathogenesis and developing effective therapeutic strategies. Although mouse models are widely used in MASLD research, they exhibit significant differences from humans in metabolic traits and disease progression, limiting their ability to fully recapitulate key pathological features of MASLD. In this study, we established an MASLD model in <i>Apodemus peninsulae</i> using a high-fat diet (HFD) and compared it with the commonly used C57BL/6J mouse model. The results showed that <i>A. peninsulae</i> developed marked lipid metabolism disorders and liver function impairment as early as week 4 with an HFD intervention. Histological analysis revealed progressive steatosis, inflammatory infiltration, and early fibrosis from weeks 8 to 16, which was confirmed by oil red O, Masson's trichrome, and Sirius red staining. In contrast, pathological progression in C57Bl/6J mice was slower, with milder fibrosis. Immunolabeling and inflammatory cytokine expression further indicated a more intense inflammatory response in <i>A. peninsulae</i>. Overall, <i>A. peninsulae</i> offers advantages, such as rapid disease induction and stable phenotypes, making it a promising animal model for studying early-stage MASLD. Its shorter modeling period and more pronounced steatosis and liver injury suggest it more closely mimics the early pathological changes seen in human MASLD.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"947-964"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147494266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}