Veterinary Pathology最新文献_第3页

MicroRNAs in serum and tissue can differentiate splenic hemangiosarcoma from other splenic masses in dogs. 血清和组织中的microrna可以区分脾血管肉瘤和其他脾肿物。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 Epub Date: 2025-02-19 DOI: 10.1177/03009858251317466

Latasha Ludwig, Heather Treleaven, Arlene Khachadoorian, Brigitte Degasperi, Ingrid Walter, Deirdre Stuart, Robert A Foster, Robert Darren Wood, R Ayesha Ali, Geoffrey A Wood

{"title":"MicroRNAs in serum and tissue can differentiate splenic hemangiosarcoma from other splenic masses in dogs.","authors":"Latasha Ludwig, Heather Treleaven, Arlene Khachadoorian, Brigitte Degasperi, Ingrid Walter, Deirdre Stuart, Robert A Foster, Robert Darren Wood, R Ayesha Ali, Geoffrey A Wood","doi":"10.1177/03009858251317466","DOIUrl":"10.1177/03009858251317466","url":null,"abstract":"Splenic masses are common in dogs and vary dramatically in their clinical behavior. Clinically, and even with histology, it can be challenging to differentiate between benign and malignant splenic masses. Hemangiosarcoma (HSA), the most common malignancy of the spleen, is a very aggressive tumor with a poor prognosis. We hypothesize that microRNAs (miRNAs) in mass tissue and serum can differentiate between HSA and other splenic masses. Fifty-nine miRNAs were investigated by reverse-transcription followed by real-time quantitative polymerase chain reaction (RT-qPCR) in serum and/or tissue from dogs with HSAs (serum n = 24 and tissue n = 17; postsplenectomy serum n = 11), lymphomas (serum n = 8 and tissue n = 11), nonangiomatous nonlymphomatous sarcomas (serum n = 6 and tissue n = 10), histiocytic sarcomas (tissue n = 4), benign splenic masses (myelolipomas, nodular hyperplasia, and hematomas; total serum n = 21 and total tissue n = 35), and normal dogs (serum n = 14 and tissue n = 7). Numerous miRNAs were differentially expressed in serum and tissue of HSA cases compared to those with other splenic masses or normal spleens. In serum, our 5-miRNA model (miR-135a-5p, miR-10a, miR-450b, miR-152-3p, and miR-126-5p) accurately classified 100% (24/24) of dogs with HSA from normal dogs and those with a benign splenic mass (recall = 1 for HSA). The overall accuracy of the model was 86%. In HSA and benign splenic mass tissues, our 3-miRNA model (miR-126-5p, miR-502-3p, and miR-452-5p) accurately classified 96% of the cases. This study demonstrates the utility of miRNA models in serum and tissue for screening and diagnosis of HSA in dogs. Future studies include the evaluation of prospective and prediagnosis serum samples.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"659-671"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143450246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital pathology in veterinary clinical pathology: A review. 数字病理学在兽医临床病理学中的应用综述。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 Epub Date: 2025-04-29 DOI: 10.1177/03009858251334340

Julie Piccione, Stephanie F Anderson, Samuel V Neal, Mara S Varvil

{"title":"Digital pathology in veterinary clinical pathology: A review.","authors":"Julie Piccione, Stephanie F Anderson, Samuel V Neal, Mara S Varvil","doi":"10.1177/03009858251334340","DOIUrl":"10.1177/03009858251334340","url":null,"abstract":"Digital pathology has rapidly evolved in the field of veterinary medicine. Although digital histology advancements are widely discussed, clinical pathology specimens are also being digitized for a variety of purposes. These digital images can be used for diagnostic evaluation, teaching, training, and research. With recent advancements in artificial intelligence technologies, there is also potential for image analysis of clinical pathology specimens. In contrast to histologic specimens, veterinary practices can process and digitize cytologic specimens in-house. This creates workflows and analytical considerations that differ greatly from the digital pathology process in anatomic pathology. Through the evaluation of published literature, conference proceedings, and commercially advertised services or analyzers, this review summarizes available digital diagnostic services, discusses virtual microscopy in education and research, and explains the concepts of image analysis. We also highlight gaps in the literature regarding digitizing clinical pathology specimens. Finally, we review the overall benefits and limitations of digitization, focusing on the unique aspects of veterinary clinical pathology.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"631-645"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathology of Clostridium colinum infection in quail. 鹌鹑柱状梭菌感染的病理研究。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 Epub Date: 2025-03-19 DOI: 10.1177/03009858251324641

Agustín Rebollada-Merino, Jey W Koehler, Martí Pumarola, Juliann Beingesser, Jessica González, Mauricio A Navarro, Francisco A Uzal

{"title":"Pathology of Clostridium colinum infection in quail.","authors":"Agustín Rebollada-Merino, Jey W Koehler, Martí Pumarola, Juliann Beingesser, Jessica González, Mauricio A Navarro, Francisco A Uzal","doi":"10.1177/03009858251324641","DOIUrl":"10.1177/03009858251324641","url":null,"abstract":"Clostridium colinum causes ulcerative enteritis in several avian species. The disease is particularly prevalent in quail, and it is therefore colloquially known as quail disease. The pathogenesis of the infection is poorly understood. A retrospective study of C. colinum infection in quail submitted for necropsy and diagnostic work up to the California Animal Health and Food Safety Laboratory System between 1992 and 2022 was performed. The necropsy reports were reviewed, and polymerase chain reaction (PCR) for C. colinum (16S rRNA) was performed on formalin-fixed, paraffin-embedded tissues. C. colinum was isolated in 17% (4/24) and detected by PCR in 96% (23/24) of cases. Bobwhite quail (Colinus virginianus) were overrepresented, and the most affected quail were juveniles. Clinical history and signs were increased mortality (92%), lethargy (29%), depression (25%), diarrhea (21%), loss of nutritional condition (8%), and seizures (8%). Grossly, intestinal ulceration (100%) affecting the duodenum (79%), jejunum (100%), ileum (29%), and/or ceca (21%). Fibrinous celomitis (13%), hepatic necrosis (46%), and pectoral muscle atrophy (92%) were observed. Histologically, all quail showed multifocal ulcerative jejunitis, duodenitis, ileitis, and/or typhlitis with intralesional bacilli. Ulcerative enteritis was transmural in 92% of cases, associated with intestinal perforation in 38%, and causing celomitis in 50% of cases. Hepatic necrosis was confirmed in 63% of cases, and neuronal changes suggesting a combination of hypoxia-ischemia and hypoglycemia were found in 63% of cases. These results suggest that a diagnosis of C. colinum infection should be made based on gross and microscopic lesions, coupled with PCR.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"793-801"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter to the Editor: Empowering pathologists with a tool to report pathology errors in publications. 给编辑的信：赋予病理学家报告出版物中病理错误的工具。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 Epub Date: 2025-06-20 DOI: 10.1177/03009858251349110

Alexandra Frankovich, Craig Miller, Kirstin Barnhart, Balasubramanian Manickam, Vimala Vemireddi, Shannon Wallace, Abigail Cox

引用次数: 0

Image challenge in Veterinary Pathology. 兽医病理学中的图像挑战。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 DOI: 10.1177/03009858251352585

引用次数: 0

Viral and squamous papillomas in captive polar bears (Ursus maritimus). 圈养北极熊的病毒和鳞状乳头状瘤。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 Epub Date: 2025-02-19 DOI: 10.1177/03009858251317478

Anna-Maria Travis, Jennifer Luff, Mandy Womble, Michael M Garner, Elise E B LaDouceur

{"title":"Viral and squamous papillomas in captive polar bears (Ursus maritimus).","authors":"Anna-Maria Travis, Jennifer Luff, Mandy Womble, Michael M Garner, Elise E B LaDouceur","doi":"10.1177/03009858251317478","DOIUrl":"10.1177/03009858251317478","url":null,"abstract":"Papillomas, many of which are virally induced, are common proliferative cutaneous and mucocutaneous lesions in multiple species, exhibiting characteristic histologic cytopathic changes that distinguish them from nonviral squamous papillomas. A single case report of a novel papillomavirus, Ursus maritimus papillomavirus-type 1, in a polar bear has been reported without investigation into any association between this virus and papilloma formation. We identified papillomas in 3 polar bears. All 3 cases had pedunculated masses consistent with papillomas (i.e., proliferative epithelium forming papillary projections on a fibrovascular stalk); case 1 also exhibited koilocytosis (cytopathic change), consistent with a viral papilloma. Polymerase chain reaction (PCR) using primers that can amplify a diversity of papillomaviruses followed by amplicon sequencing yielded a novel papillomavirus sequence in case 1, which shared <70% nucleotide identity to any known papillomavirus type, indicative of a putatively novel papillomavirus. In situ hybridization (ISH) of case 1 demonstrated viral nucleic acid within proliferative cells and not within the adjacent normal skin, suggesting the virus was the causative agent of this papilloma. The squamous papillomas in cases 2 and 3 were negative for papillomavirus by both PCR and ISH. These findings support our hypothesis that cytopathic effect is associated with the presence of papillomavirus in polar bears, while the lack of histologic cytopathic change may predict nonviral pathogenesis. Further sequencing of the putatively novel viral genome will benefit research and conservation efforts of polar bears.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"767-772"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143450292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic bronchitis and bronchiolitis with prominent globule leukocyte infiltration in a cat with Filobacterium felis. 猫丝状杆菌感染的慢性支气管炎和细支气管炎伴明显的白细胞浸润。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 Epub Date: 2025-03-15 DOI: 10.1177/03009858251324646

Takanori Shiga, Yoko Kakinuma, Miu Takada, Rie Imai, Takuma Aoki, Hiroho Ishida, Miwako Kasahara-Kamiie, Naoyuki Aihara, Junichi Kamiie

{"title":"Chronic bronchitis and bronchiolitis with prominent globule leukocyte infiltration in a cat with Filobacterium felis.","authors":"Takanori Shiga, Yoko Kakinuma, Miu Takada, Rie Imai, Takuma Aoki, Hiroho Ishida, Miwako Kasahara-Kamiie, Naoyuki Aihara, Junichi Kamiie","doi":"10.1177/03009858251324646","DOIUrl":"10.1177/03009858251324646","url":null,"abstract":"A 7-year-old mixed-breed cat presented with intermittent dry cough for 2 years. Histological examination of the lung biopsy revealed hyperplasia of bronchial and bronchiolar epithelium, and intraepithelial infiltration of globule leukocytes in many bronchi, bronchioles, and terminal bronchioles. Lymphoid aggregates cuffed airways, respiratory bronchioles, and alveolar ducts. Terminal airway inflammation was associated with type II pneumocyte hyperplasia within adjacent alveoli. Warthin-Starry stain revealed numerous argyrophilic and filamentous bacilli that were interspersed or clustered with cilia of the respiratory epithelium. The morphology, distribution, and gram (negative) and ultrastructural characteristics were consistent with cilia-associated respiratory (CAR) bacillus. Real-time polymerase chain reaction assays using paraffin-embedded lung tissue confirmed the presence of Filobacterium felis. Chronic bronchitis and bronchiolitis in a cat with F. felis share characteristics of diseases caused by species specific CAR bacilli in many species, but uniquely in this case, globule leukocytes were a prominent feature of the inflammatory response.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"762-766"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image challenge in Veterinary Pathology, answers: Fish diseases. 兽医病理学中的图像挑战，答案：鱼类疾病。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 DOI: 10.1177/03009858251352584

引用次数: 0

Immunohistochemical characterization of differentiation-associated transcription factors, tumor suppressor genes, and mismatch repair genes in rabbit uterine adenocarcinoma. 兔子宫腺癌中分化相关转录因子、肿瘤抑制基因和错配修复基因的免疫组化特征。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 Epub Date: 2025-04-21 DOI: 10.1177/03009858251332005

Yukino Machida, Sayuri Harashima, Viviana Gonzalez Astudillo, Masaki Michishita

{"title":"Immunohistochemical characterization of differentiation-associated transcription factors, tumor suppressor genes, and mismatch repair genes in rabbit uterine adenocarcinoma.","authors":"Yukino Machida, Sayuri Harashima, Viviana Gonzalez Astudillo, Masaki Michishita","doi":"10.1177/03009858251332005","DOIUrl":"10.1177/03009858251332005","url":null,"abstract":"The development of human uterine cancer is a complex process involving the abnormal expression of tumor suppressors, such as PTEN, ARID1A, and TP53; mismatch repair protein MSH6; and transcription factors, such as PAX2 and PAX8. The functional changes that lead to uterine adenocarcinoma in pet rabbits are not fully understood despite the frequent occurrence of this condition in the species. Thus, an immunohistochemical analysis was performed to visualize the protein expression pattern of carcinogenesis-related molecules in surgical biopsy specimens from 56 uterine adenocarcinomas and 8 uteruses without significant lesions in pet rabbits. Seventy percent of the adenocarcinomas were positive for estrogen receptor (ER), 7% were positive for TP53, and there was a decreased expression in comparison to control uterine epithelium for PAX2 in 54%, for PAX8 in 73%, for ARID1A in 68%, and for MSH6 in 48% of the tumors. TP53 expression was not observed in control uterine tissues. There was a significant negative correlation between nuclear ER and PAX2 immunolabeling in uterine adenocarcinomas. Heat map analysis classified samples into 4 clusters, which revealed that 1 PAX2-positive group had a higher presence of papillary-type uterine adenocarcinomas and a lower prevalence of tubular/solid types compared with the 2 PAX2-negative groups. This study demonstrated that the immunohistochemical phenotype of rabbit uterine adenocarcinoma is comparable to that of human endometrial carcinomas, suggesting the potential for similar oncogenic mechanisms that might prove useful for translational medicine research.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"683-691"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinicopathological progression and molecular characterization of intestinal dilatation syndrome in commercial brown layers. 商品褐蛋鸡肠扩张综合征的临床病理进展及分子特征。

IF 1.7 2区农林科学

Veterinary Pathology Pub Date : 2025-09-01 Epub Date: 2025-04-11 DOI: 10.1177/03009858251331106

Álex Gómez, Ana Rodríguez-Largo, Estela Pérez, Serafín García Freire, Christiane Hundehege, Eva Berberich, Lluís Luján, Diego Cortés

{"title":"Clinicopathological progression and molecular characterization of intestinal dilatation syndrome in commercial brown layers.","authors":"Álex Gómez, Ana Rodríguez-Largo, Estela Pérez, Serafín García Freire, Christiane Hundehege, Eva Berberich, Lluís Luján, Diego Cortés","doi":"10.1177/03009858251331106","DOIUrl":"10.1177/03009858251331106","url":null,"abstract":"Intestinal dilatation syndrome (IDS) is a poorly described condition affecting layers and breeder hens globally. Its prevalence is increasing, particularly in free-range systems, but the cause remains unknown. This retrospective study examined 35 hens from 3 flocks: free-range flock A (n = 20) and enriched-caged flock B (n = 5), both affected by IDS, and enriched-caged flock C (n = 10), with no history of IDS. Clinicopathological studies were performed on these hens, and metagenomic analysis was conducted on the proventriculus and jejunum of hens from flock A (n = 2) and flock C (n = 2). Based on clinical signs and lesions, 3 progressive stages of IDS were identified. In the first stage, although hens were without clinical signs, proventricular dilatation and lymphoplasmacytic and heterophilic jejunitis and duodenitis were observed. The second stage was marked by cachexia, pale and small combs and wattles, and severe egg production drop. Jejunal dilatation was observed, with microscopic evidence of necrotic, lymphoplasmacytic and heterophilic jejunitis; ganglioneuritis; and mineralization of the jejunal nervous plexuses and subserosal ganglia. In the third stage, spontaneous death occurred due to jejunal volvulus and vascular involvement. Affected hens (stage 2) also exhibited elevated cloacal temperatures (>0.9°C) and marked heterophilia. Metagenomic analysis identified sequences consistent with Megrivirus C in IDS-affected hens and a disruption of the gut microbiota, with increased abundance of Fusobacterium mortiferum and Megamonas funiformis. In conclusion, this study describes in detail the clinicopathological progression of the IDS and suggests that Megrivirus C, in combination with opportunistic intestinal bacteria, could play a role in the pathogenesis of this disease.","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"740-747"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0