Virus research最新文献

{"title":"Identification of two novel B cell epitopes on E184L protein of African swine fever virus using monoclonal antibodies","authors":"Weldu Tesfagaber ,&nbsp;Desong Lan ,&nbsp;Wan Wang ,&nbsp;Rui Zhao ,&nbsp;Li Yin ,&nbsp;Mingyang Yang ,&nbsp;Yuanmao Zhu ,&nbsp;Encheng Sun ,&nbsp;Renqiang Liu ,&nbsp;Wenjun Lin ,&nbsp;Zhigao Bu ,&nbsp;Fang Li ,&nbsp;Dongming Zhao","doi":"10.1016/j.virusres.2024.199412","DOIUrl":"10.1016/j.virusres.2024.199412","url":null,"abstract":"<div><p>African swine fever virus (ASFV) is a large double-stranded DNA virus with a complex structural architecture and encodes more than 150 proteins, where many are with unknown functions. E184L has been reported as one of the immunogenic ASFV proteins that may contribute to ASFV pathogenesis and immune evasion. However, the antigenic epitopes of E184L are not yet characterized. In this study, recombinant E184L protein was expressed in prokaryotic expression system and four monoclonal antibodies (mAbs), designated as 1A10, 2D2, 3H6, and 4C10 were generated. All four mAbs reacted specifically with ASFV infected cells. To identify the epitopes of the mAbs, a series of overlapped peptides of E184L were designed and expressed as maltose binding fusion proteins. Accordingly, the expressed fusion proteins were probed with each E184L mAb separately by using Western blot. Following a fine mapping, the minimal linear epitope recognized by mAb 1A10 was identified as ¹¹⁹IQRQGFL¹²⁵, and mAbs 2D2, 3H6, and 4C10 recognized a region located between ¹⁵³DPTEFF¹⁵⁸. Alignment of amino acids of E184L revealed that the two linear epitopes are highly conserved among different ASFV isolates. Furthermore, the potential application of the two epitopes in ASFV diagnosis was assessed through epitope-based ELISA using 24 ASFV positive and 18 negative pig serum and the method were able to distinguish positive and negative samples, indicating the two epitopes are dominant antigenic sites. To our knowledge, this is the first study to characterize the B cell epitopes of the antigenic E184L protein of ASFV, offering valuable tools for future research, as well as laying a foundation for serological diagnosis and epitope-based marker vaccine development.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001059/pdfft?md5=f304a58f4304975c187e2e5f8c077f4f&pid=1-s2.0-S0168170224001059-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141262987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating rotavirus strains in children with acute gastroenteritis in Iran, 1986 to 2023 and their genetic/antigenic divergence compared to approved vaccines strains (Rotarix, RotaTeq, ROTAVAC, ROTASIIL) before mass vaccination: Clues for vaccination policy makers","authors":"Somayeh Jalilvand ,&nbsp;Tayebeh Latifi ,&nbsp;Atefeh Kachooei ,&nbsp;Mahtab Mirhoseinian ,&nbsp;Somayeh-Sadat Hoseini-Fakhr ,&nbsp;Farzane Behnezhad ,&nbsp;Farzin Roohvand ,&nbsp;Zabihollah Shoja","doi":"10.1016/j.virusres.2024.199411","DOIUrl":"10.1016/j.virusres.2024.199411","url":null,"abstract":"<div><p>In the present study, first, rotaviruses that caused acute gastroenteritis in children under five years of age during the time before the vaccine was introduced in Iran (1986 to 2023) are reviewed. Subsequently, the antigenic epitopes of the VP7 and VP4/VP8 proteins in circulating rotavirus strains in Iran and that of the vaccine strains were compared and their genetic differences in histo-blood group antigens (HBGAs) and the potential impact on rotavirus infection susceptibility and vaccine efficacy were discussed. Overall data indicate that rotavirus was estimated in about 38.1 % of samples tested. The most common genotypes or combinations were G1 and P[8], or G1P[8]. From 2015 to 2023, there was a decline in the prevalence of G1P[8], with intermittent peaks of genotypes G3P[8] and G9P[8]. The analyses suggested that the monovalent Rotarix vaccine or monovalent vaccines containing the G1P[8] component might be proper in areas with a similar rotavirus genotype pattern and genetic background as the Iranian population where the G1P[8] strain is the most predominant and has the ability to bind to HBGA secretors. While the same concept can be applied to RotaTeq and RotasIIL vaccines, their complex vaccine technology, which involves reassortment, makes them less of a priority. The ROTASIIL vaccine, despite not having the VP4 arm (P[5]) as a suitable protection option, has previously shown the ability to neutralize not only G9-lineage I strains but also other G9-lineages at high titers. Thus, vaccination with the ROTASIIL vaccine may be more effective in Iran compared to RotaTeq. However, considering the rotavirus genotypic pattern, ROTAVAC might not be a good choice for Iran. Overall, the findings of this study provide valuable insights into the prevalence of rotavirus strains and the potential effectiveness of different vaccines in the Iranian and similar populations.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001047/pdfft?md5=8a76234069020899169a588ebf45d19a&pid=1-s2.0-S0168170224001047-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141185786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal analysis of the enteric virome in paediatric subjects from the Free State Province, South Africa, reveals early gut colonisation and temporal dynamics","authors":"Milton Tshidiso Mogotsi ,&nbsp;Ayodeji Emmanuel Ogunbayo ,&nbsp;Phillip Armand Bester ,&nbsp;Hester Gertruida O'Neill ,&nbsp;Martin Munene Nyaga","doi":"10.1016/j.virusres.2024.199403","DOIUrl":"10.1016/j.virusres.2024.199403","url":null,"abstract":"<div><p>The gut of healthy neonates is devoid of viruses at birth, but rapidly becomes colonised by normal viral commensals that aid in important physiological functions like metabolism but can, in some instances, result in gastrointestinal illnesses. However, little is known about how this colonisation begins, its variability and factors shaping the gut virome composition. Thus, understanding the development, assembly, and progression of enteric viral communities over time is key. To explore early-life virome development, metagenomic sequencing was employed in faecal samples collected longitudinally from a cohort of 17 infants during their first six months of life. The gut virome analysis revealed a diverse and dynamic viral community, formed by a richness of different viruses infecting humans, non-human mammals, bacteria, and plants. Eukaryotic viruses were detected as early as one week of life, increasing in abundance and diversity over time. Most of the viruses detected are commonly associated with gastroenteritis and include members of the <em>Caliciviridae, Picornaviridae, Astroviridae, Adenoviridae</em>, and <em>Sedoreoviridae</em> families. The most common co-occurrences involved asymptomatic norovirus-parechovirus, norovirus-sapovirus, sapovirus-parechovirus, observed in at least 40 % of the samples. Majority of the plant-derived viruses detected in the infants’ gut were from the <em>Virgaviridae</em> family. This study demonstrates the first longitudinal characterisation of the gastrointestinal virome in infants, from birth up to 6 months of age, in sub-Saharan Africa. Overall, the findings from this study delineate the composition and variability of the healthy infants’ gut virome over time, which is a significant step towards understanding the dynamics and biogeography of viral communities in the infant gut.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224000960/pdfft?md5=9097011694ce54c9a7894e3bbea7730e&pid=1-s2.0-S0168170224000960-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141082337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Convalescent human plasma candidate reference materials protect against Crimean-Congo haemorrhagic fever virus (CCHFV) challenge in an A129 mouse model","authors":"Sarah Kempster ,&nbsp;Mark Hassall ,&nbsp;Victoria Graham ,&nbsp;Emma Kennedy ,&nbsp;Stephen Findlay-Wilson ,&nbsp;Francisco J. Salguero ,&nbsp;Binnur Bagci ,&nbsp;Nazif Elaldi ,&nbsp;Murtaza Oz ,&nbsp;Tuba Tasseten ,&nbsp;Frank W. Charlton ,&nbsp;John N. Barr ,&nbsp;Juan Fontana ,&nbsp;Chinwe Duru ,&nbsp;Ernest Ezeajughi ,&nbsp;Paul Matejtschuk ,&nbsp;Ulrike Arnold ,&nbsp;Yemisi Adedeji ,&nbsp;Ali Mirazimi ,&nbsp;Roger Hewson ,&nbsp;Neil Almond","doi":"10.1016/j.virusres.2024.199409","DOIUrl":"10.1016/j.virusres.2024.199409","url":null,"abstract":"<div><p>Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is spread by infected ticks or direct contact with blood, tissues and fluids from infected patients or livestock. Infection with CCHFV causes severe haemorrhagic fever in humans which is fatal in up to 83 % of cases. CCHFV is listed as a priority pathogen by the World Health Organization (WHO) and there are currently no widely-approved vaccines. Defining a serological correlate of protection against CCHFV infection would support the development of vaccines by providing a ‘target threshold’ for pre-clinical and clinical immunogenicity studies to achieve in subjects and potentially obviate the need for <em>in vivo</em> protection studies. We therefore sought to establish titratable protection against CCHFV using pooled human convalescent plasma, in a mouse model. Convalescent plasma collected from seven individuals with a known previous CCHFV virus infection were characterised using binding antibody and neutralisation assays. All plasma recognised nucleoprotein and the Gc glycoprotein, but some had a lower Gn glycoprotein response by ELISA. Pooled plasma and two individual donations from convalescent donors were administered intraperitoneally to A129 mice 24 h prior to intradermal challenge with CCHFV (strain IbAr10200). A partial protective effect was observed with all three convalescent plasmas characterised by longer survival post-challenge and reduced clinical score. These protective responses were titratable. Further characterisation of the serological reactivities within these samples will establish their value as reference materials to support assay harmonisation and accelerate vaccine development for CCHFV.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001023/pdfft?md5=60a8662c3f17b58c299068a3d68bc93a&pid=1-s2.0-S0168170224001023-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Coronavirus helicase in replication","authors":"Samantha L. Grimes,&nbsp;Mark R. Denison","doi":"10.1016/j.virusres.2024.199401","DOIUrl":"10.1016/j.virusres.2024.199401","url":null,"abstract":"<div><p>The coronavirus nonstructural protein (nsp) 13 encodes an RNA helicase (nsp13-HEL) with multiple enzymatic functions, including unwinding and nucleoside phosphatase (NTPase) activities. Attempts for enzymatic inactivation have defined the nsp13-HEL as a critical enzyme for viral replication and a high-priority target for antiviral development. Helicases have been shown to play numerous roles beyond their canonical ATPase and unwinding activities, though these functions are just beginning to be explored in coronavirus biology. Recent genetic and biochemical studies, as well as work in structurally-related helicases, have provided evidence that supports new hypotheses for the helicase's potential role in coronavirus replication. Here, we review several aspects of the coronavirus nsp13-HEL, including its reported and proposed functions in viral replication and highlight fundamental areas of research that may aid the development of helicase inhibitors.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224000947/pdfft?md5=9aef7b1c9d303096b6d5816e4bcf6a31&pid=1-s2.0-S0168170224000947-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous and ultrafast detection of pan-SARS-coronaviruses and influenza A/B viruses by a novel multiplex real-time RT-PCR assay","authors":"Changping Xu ,&nbsp;Zhengyang Wang ,&nbsp;Beibei Yu ,&nbsp;Zhenhuang Pan ,&nbsp;Jun Ni ,&nbsp;Yan Feng ,&nbsp;Shiwang Huang ,&nbsp;Maomao Wu ,&nbsp;Jiancang Zhou ,&nbsp;Lei Fang ,&nbsp;Zhiwei Wu","doi":"10.1016/j.virusres.2024.199410","DOIUrl":"10.1016/j.virusres.2024.199410","url":null,"abstract":"<div><p>Here we report an ultrafast quadruplex RT-qPCR assay with robust diagnostic ability to detect and distinguish pan-SARS-CoVs and influenza A/B viruses within 35 min. This quadruplex RT-qPCR assay comprised of one novel RNA-based internal control targeting human β2-microglobulin (<em>B2M</em>) for process accuracy and three newly-designed primers-probe sets targeting the envelope protein (<em>E</em>) of pan-SARS-CoV, matrix protein (<em>MP</em>) of influenza A virus and non-structural (<em>NS</em>) region of influenza B virus. This quadruplex assay exhibited a sensitivity comparable to its singleplex counterparts and a slightly higher to that of the Centers for Disease Control and Prevention-recommended SARS-CoV-2 and influenza A/B assays. The novel assay showed no false-positive amplifications with other common respiratory viruses, and its 95 % limits of detection for pan-SARS-CoV and influenza A/B virus was 4.26–4.52 copies/reaction. Moreover, the assay was reproducible with less than 1 % coefficient of variation and adaptable testing different clinical and environmental samples. Our ultrafast quadruplex RT-qPCR assay can serve as an attractive tool for effective differentiation of influenza A/B virus and SARS-CoV-2, but more importantly prognose the reemergence/emergence of SARS and novel coronaviruses or influenza viruses from animal spillover.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001035/pdfft?md5=d09a3c3ddb5d508366021282c790ad4e&pid=1-s2.0-S0168170224001035-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of a pseudolysogenic phage vB_YpM_HQ103 infecting Yersinia pestis","authors":"Zijian Wang ,&nbsp;Jiao Yang,&nbsp;Lihua Yang,&nbsp;Youhong Zhong,&nbsp;Peng Wang","doi":"10.1016/j.virusres.2024.199395","DOIUrl":"10.1016/j.virusres.2024.199395","url":null,"abstract":"<div><p>The plague, caused by <em>Yersinia pestis</em>, is a natural focal disease and the presence of <em>Y. pestis</em> in the environment is a critical ecological concern worldwide. The role of <em>Y. pestis</em> phages in the ecological life cycle of the plague is crucial. Previously, a temperature-sensitive phage named vB_YpM_HQ103 was isolated from plague foci in Yunnan province, China. Upon infecting the EV76 strain of <em>Y. pestis</em>, vB_YpM_HQ103 exhibits lysogenic behavior at 21 °C and lytic behavior at 37 °C. Various methods including continuous passage lysogenic tests, in vitro lysis tests, comparative genomic assays, fluorescence quantitative PCR and receptor identification tests were employed to demonstrate that the lysogenic life cycle of this phage is applicable to wild <em>Y. pestis</em> strains; its lysogeny is pseudolysogenic (carrying but not integrating), allowing it to replicate and proliferate within <em>Y. pestis</em>. Furthermore, we have identified the outer membrane protein OmpA of <em>Y. pestis</em> as the receptor for phage infection. In conclusion, our research provides insight into the characteristics and receptors of a novel <em>Y. pestis</em> phage infection with a pseudolysogenic cycle. The findings of this study enhance our understanding of <em>Y. pestis</em> phages and plague microecology, offering valuable insights for future studies on the conservation and genetic evolution of <em>Y. pestis</em> in nature.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224000881/pdfft?md5=ffe49070c8e66a86001137b0fe86aa37&pid=1-s2.0-S0168170224000881-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying SNP threshold from P2 sequences for investigating norovirus transmission","authors":"Luqiu Tao ,&nbsp;Xuan Wang ,&nbsp;Yan Yu ,&nbsp;Teng Ge ,&nbsp;Hongjin Gong ,&nbsp;Wei Yong ,&nbsp;Jiali Si ,&nbsp;Min He ,&nbsp;Jie Ding","doi":"10.1016/j.virusres.2024.199408","DOIUrl":"10.1016/j.virusres.2024.199408","url":null,"abstract":"<div><p>Noroviruses are a group of non-enveloped single-stranded positive-sense RNA virus belonging to Caliciviridae family. They can be transmitted by the fecal-oral route from contaminated food and water and cause mainly acute gastroenteritis. Outbreaks of norovirus infections could be difficult to detect and investigate. In this study, we developed a simple threshold detection approach based on variations of the P2 domain of the capsid protein. We obtained sequences from the norovirus hypervariable P2 region using Sanger sequencing, including 582 pairs of epidemiologically-related strains from 35 norovirus outbreaks and 6402 pairs of epidemiologically-unrelated strains during the four epidemic seasons. Genetic distances were calculated and a threshold was performed by adopting ROC (Receiver Operating Characteristic) curve which identified transmission clusters in all tested outbreaks with 80 % sensitivity. In average, nucleotide diversity between outbreaks was 67.5 times greater than the diversity within outbreaks. Simple and accurate thresholds for detecting norovirus transmissions of three genotypes obtained here streamlines molecular investigation of norovirus outbreaks, thus enabling rapid and efficient responses for the control of norovirus.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001011/pdfft?md5=0045dfe44510f207a5a7aeaeb4da4be0&pid=1-s2.0-S0168170224001011-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parapoxvirus species revisited by whole genome sequencing: A retrospective analysis of bovine virus isolates","authors":"Graf Alexander ,&nbsp;Rziha Hanns-Joachim ,&nbsp;Krebs Stefan ,&nbsp;Wolf Eckhard ,&nbsp;Blum Helmut ,&nbsp;Büttner Mathias","doi":"10.1016/j.virusres.2024.199404","DOIUrl":"10.1016/j.virusres.2024.199404","url":null,"abstract":"<div><p><em>Parapoxviruses</em> (PPV) of animals are spread worldwide. While the <em>Orf virus</em> (ORFV) species is a molecularly well-characterized prototype pathogen of small ruminants, the genomes of virus species affecting large ruminants, namely <em>Bovine papular stomatitis virus</em> (BPSV) and <em>Pseudocowpox virus</em> (PCPV), are less well known. Using Nanopore sequencing we retrospectively show the whole genome sequences (WGS) of six BPSV, three PCPV isolates and an attenuated ORFV strain, originating from different geographic locations. A phylogenetic tree shows that the de novo assembled genomes belong to PPV species including WGS of reference PPV. Remarkably, Nanopore sequencing allowed the molecular resolution of inverted terminal repeats (ITR) and the hairpin loop within the de novo assembled WGS. Additionally, peculiarities regarding map location of two genes and the heterogeneity of a genomic region were noted. Details for the molecular variability of an interferon response modulatory gene (ORF116) and the PCPV specificity of gene 073.5 are reported. In summary, WGS gained by Nanopore sequencing allowed analysis of complete PPV genomes and confident virus species attribution within a phylogenetic tree avoiding uncertainty of limited gene-based diagnostics. Nanopore-based WGS provides robust comparison of PPV genomes and reliable identity determination of new Poxviruses.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224000972/pdfft?md5=322cc58ed3a2e51ee3918ad80ef9a6c3&pid=1-s2.0-S0168170224000972-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141089003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T-cell immunoglobulin and mucin 1 (TIM-1) mediates infection of Hantaan virus in Jurkat T cells","authors":"Ruixue Ma ,&nbsp;Xuyang Zheng ,&nbsp;Tianle Gu ,&nbsp;Ziyu Liu ,&nbsp;Shiyuan Hou ,&nbsp;Danni Sun ,&nbsp;Yaxin Ding ,&nbsp;Fang Wang ,&nbsp;Qikang Ying ,&nbsp;Xiaohan Ma ,&nbsp;Huarui Kang ,&nbsp;Rongrong Liu ,&nbsp;Jianqi Lian ,&nbsp;Xingan Wu","doi":"10.1016/j.virusres.2024.199394","DOIUrl":"10.1016/j.virusres.2024.199394","url":null,"abstract":"<div><p>Hantaan virus (HTNV) is a major public health concern due to its ability to cause hemorrhagic fever with renal syndrome (HFRS) in Eurasia. Symptoms of HFRS include fever, hemorrhage, immune dysfunction and renal impairment, and severe cases can be fatal. T cell-mediated adaptive immune responses play a pivotal role in countering HTNV infection. However, our understanding of HTNV and T cell interactions in the disease progression is limited. In this study, we found that human CD4⁺ T cells can be directly infected with HTNV, thereby facilitating viral replication and production. Additionally, T-cell immunoglobulin and mucin 1 (TIM-1) participated in the process of HTNV infection of Jurkat T cells, and further observed that HTNV enters Jurkat T cells via the clathrin-dependent endocytosis pathway. These findings not only affirm the susceptibility of human CD4⁺ T lymphocytes to HTNV but also shed light on the viral tropism. Our research elucidates a mode of the interaction between the virus infection process and the immune system. Critically, this study provides new insights into the pathogenesis of HTNV and the implications for antiviral research.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S016817022400087X/pdfft?md5=9bfe77e4fbbb6bd0acd3baabac982f06&pid=1-s2.0-S016817022400087X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}