Andrea Sierra-Mejia, Dan E V Villamor, Ioannis E Tzanetakis
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引用次数: 0
Abstract
Blackberry chlorotic ringspot virus (BCRV) was described about 20 years ago and since then there have been several publications of the virus infecting rosaceous hosts including blackberry, raspberry, rose and apple at high rates. Still the effect of the virus on disease development is poorly understood. Aiming to bridge this knowledge gap, we developed a BCRV infectious clone and virus-induced gene silencing vector (VIGS). The infectious clone can induce systemic infection with the transmissibility of the recombinant virus evaluated through mechanical transmission. The VIGS induced silencing using two different inserts, proving the versatility of the construct. The products of this work can be used to study disease development and control as well as functional genomics studies of BCRV hosts.
期刊介绍：
Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.
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