Virus research最新文献

{"title":"Exploration of mycovirus composition in a hypovirulent strain of Sclerotinia sclerotiorum potentially uncovers mycovirus cross-taxa transmission.","authors":"Lixia Gao, Weimeng Li, Jichun Jia, Jiasen Cheng, Yanping Fu, Xueqiong Xiao, Qing Cai, Yang Lin, Tao Chen, Bo Li, Xiao Yu, Tom Hsiang, Daohong Jiang, Jiatao Xie","doi":"10.1016/j.virusres.2025.199552","DOIUrl":"https://doi.org/10.1016/j.virusres.2025.199552","url":null,"abstract":"<p><p>Sclerotinia sclerotiorum is a worldwide plant pathogenic fungus. Identifying novel mycoviruses in this fungus can aid in developing fungal disease control strategies and enhance our understanding of viral evolution. Here, we analyzed mycovirus composition in S. sclerotiorum strain XZ69, and identified six ssRNA mycoviruses, including five known mycoviruses and one unassigned mycovirus. The newly identified mycovirus, tentatively named Sclerotinia sclerotiorum narna-like virus 1 (SsNLV1/XZ69), possesses a full-length genome of 3534 nucleotides, containing a single ORF that encodes an RNA-dependent RNA polymerase (RdRp) of 1090 amino acids. The RdRp encoded by SsNLV1/XZ69 shares 60.4% identity with that encoded by Monilinia narnavirus H. SsNLV1/XZ69 phylogenetically clusters with unclassified narna-like viruses potentially infecting fungi, plants, and animals, and they form an independent branch that is distant from established families, therefore supporting the establishment of a new family to accommodate these viruses. Sclerotinia sclerotiorum fusarivirus 3 (SsFV3/XZ69) share 97% amino acid identities with preciously reported Botrytis cinerea fusarivirus 8 (BcFV8). This last mycovirus originated from Botrytis cinerea, and hence this reveals that cross-genus transmission of SsFV3 or BcFV8 between B. cinerea and S. sclerotiorum may have potentially occurred. Mycovirus elimination, horizontal transmission, and RNA transfection experiments revealed that Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV1/XZ69), SsNSRV2/XZ69, and SsFV3/XZ69 may be associated with hypovirulence in S. sclerotiorum, and strain XZ69 exhibits potential disease biocontrol on rapeseed seedlings. Our study expands our understanding of viral evolution, and may provide new potential biocontrol agents for S. sclerotiorum.</p>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":" ","pages":"199552"},"PeriodicalIF":2.5,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-transcriptomic sequencing reveals divergent RNA viruses in geckos","authors":"Shuqi Liu ,&nbsp;Ruiling Niu ,&nbsp;Xinrui Wang ,&nbsp;Jingxuan Cui ,&nbsp;Mingxue Cui ,&nbsp;Hong Zhou ,&nbsp;Juan Li ,&nbsp;Edward C Holmes ,&nbsp;Weifeng Shi ,&nbsp;Cixiu Li","doi":"10.1016/j.virusres.2025.199551","DOIUrl":"10.1016/j.virusres.2025.199551","url":null,"abstract":"<div><div>Geckos are generally small, predominantly nocturnal reptiles, with several species commonly found close to human habitations. However, little is known about viral diversity in geckos. Using meta-transcriptomic sequencing we identified four novel RNA viruses – provisionally denoted Gecko astrovirus, Gecko parechovirus, Gecko reptillovirus, and Gecko hartmanivirus – in geckos sampled in October 2019 from Hainan Province, China. The presence of these viruses was confirmed by reverse transcription (RT)-PCR. Phylogenetic analyses revealed that these viruses were most closely related to those identified in various gecko species from China and Australia, such that they represent gecko-specific lineages, yet were also genetically distinct, with amino acid sequence identities to their closest relatives ranging from 38.6 % to 74.2 %. A co-phylogeny analysis revealed a complex interplay between long-term virus-host co-divergence and more recent host jumping, which differed in frequency among groups. In sum, we demonstrate the presence of four novel gecko-associated RNA viruses, expanding our understanding of viral diversity in these common animal species.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199551"},"PeriodicalIF":2.5,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-existing Anti-AAV9 Antibodies in the Chinese Healthy and Rare Disease Populations: Implications for Gene Therapy.","authors":"Qian Zhao, Shuangqing Yu, Diyi Fu, Zhen Wu, Jianfang Zhou, Yi Yang, Chen Chen, Ni Wu, Yucan Wang, Wanlin Xi, Ning Lou, Xiaobing Wu, Xiaohong Han","doi":"10.1016/j.virusres.2025.199549","DOIUrl":"https://doi.org/10.1016/j.virusres.2025.199549","url":null,"abstract":"<p><p>The adeno-associated virus 9 (AAV9) vector was particularly notable for its broad tissue tropism, making it a preferred vector for gene therapy. Goals: The study aimed to investigate the patterns of pre-existing immunity against AAV9 in the Chinese population. In this study, we conducted a serological research from November 2022 to June 2024. The study included 341 participants in total with age ranged from 0-90 years old: 270 healthy individuals, 30 pediatric patients and 41 adults with rare diseases. Total AAV9-binding antibodies (TAbs) and neutralizing antibodies (NAbs) were measured. The seroprevalence of anti-AAV9 NAbs showed no significant differences between healthy individuals and rare disease patients across both pediatric and adult groups. Newborns exhibited a high NAb-positive rate (64.3%), while children aged 6 months to 3 years had the lowest prevalence (7.7%). This rate progressively increased through childhood and adolescence. Overall, 58.7% of the Chinese population aged 0-90 years tested positive for anti-AAV9 NAbs, with adults showing a significantly higher prevalence than children (75.0% vs. 34.3%). Additionally, 58.1% of the population exhibited low levels of anti-AAV9 NAb titers (IC₅₀ ≤ 100). No significant sex-specific differences were observed, and antibody titers (NAbs or TAbs) showed no strong correlation with age. A strong correlation was identified between TAb and NAb positivity rates and titers. The optimal AAV9-based GT period was between 6 months and 3 years in that patients possessed lowest pre-existing immunity. Since TAbs had a strong association with NAbs, TAbs was considered as an alternative indicator to screen rare diseases.</p>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":" ","pages":"199549"},"PeriodicalIF":2.5,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adeno-associated virus 2 CRISPR/Cas9-mediated targeting of hepatitis B virus in tree shrews","authors":"Md Haroon Or Rashid ,&nbsp;Mohammad Enamul Hoque Kayesh ,&nbsp;Md Abul Hashem ,&nbsp;Tatsuro Hifumi ,&nbsp;Shintaro Ogawa ,&nbsp;Noriaki Miyoshi ,&nbsp;Yasuhito Tanaka ,&nbsp;Michinori Kohara ,&nbsp;Kyoko Tsukiyama-Kohara","doi":"10.1016/j.virusres.2025.199550","DOIUrl":"10.1016/j.virusres.2025.199550","url":null,"abstract":"<div><div>Chronic hepatitis B virus (HBV) infection is a global health issue with limited therapeutic options given the persistence of viral episomal DNA (cccDNA). Previously, we investigated the effects of adeno-associated virus 2 (AAV2) vector-mediated delivery of three guide (g)RNAs/Cas9 selected from 16 gRNAs. AAV2/WJ11-Cas9 effectively suppressed HBV replication <em>in vitro</em> and in humanized chimeric mouse livers. In the present study, we examined the effect of AAV2/WJ11-Cas9 on the acute phase of HBV genotype F infection in an immunocompetent northern tree shrew (<em>Tupaia belangeri</em>; hereafter, “tupaia”) model. AAV2/WJ11-Cas9 treatment significantly reduced the HBV viral load in serum at 1, 7, 10, and 14 days post-infection (dpi). HBV-F infection caused enlargement of hepatocytes and mild lymphocytic infiltration in the interlobular connective tissue. Thus, the virus damages hepatocytes and drives infection progression and HBV core antigen (HBcAg) accumulation, which were not observed in AAV2/WJ11-Cas9 treated and normal liver tissues. AAV2/WJ11-Cas9 treatment reduced HBV DNA and cccDNA in liver tissues, as well as serum levels of HBV surface antigen and HBV core-related antigen (HBcrAg), including HBcAg and HBeAg at 14 dpi. Anti-HBc, anti-HBs, and anti-AAV Abs production was also detected. AAV2/WJ11-Cas9 treatment suppressed inflammatory cytokines and <em>TLR1, TLR2, TLR3, TLR4, TLR6, TLR7</em>, and <em>TLR9</em> mRNA levels. Thus, WJ11/Cas9 delivered by AAV2 vectors may provide a new therapeutic approach for inhibiting HBV infection in immunocompetent animal models, which could be developed for use in humans through further translational research.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199550"},"PeriodicalIF":2.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel rhabdovirus persistently infects lepidopteran cell lines","authors":"Takeo Fukushima ,&nbsp;Shohei Takamura ,&nbsp;Keisuke Shoji ,&nbsp;Tomoyo Touguchi ,&nbsp;Susumu Katsuma ,&nbsp;Masashi Iwanaga","doi":"10.1016/j.virusres.2025.199548","DOIUrl":"10.1016/j.virusres.2025.199548","url":null,"abstract":"<div><div>A novel rhabdovirus was identified in BmN-4 (BmN) cells derived from <em>Bombyx mori</em> and Sf9 cells derived from <em>Spodoptera frugiperda</em>. Genome sequence homology revealed that this novel virus is distinct from the previously reported Spodoptera frugiperda rhabdovirus (SfRV). Given the high similarity of the <em>large protein</em> sequence of this virus to that of the Taarstrup virus, we named it Bombyx mori taarstrup virus (BmTV). When BmTV-negative <em>B. mori</em>-derived cultured cells were inoculated with BmTV, an increase in virus RNA was observed, but no impact on host cell proliferation occurred, suggesting that BmTV exhibits latent infection in insect cells. On the other hand, oral and hemocoel inoculation in <em>B. mor</em>i larvae did not cause significant increase in BmTV replication. After subcutaneous inoculation, no difference in adult metamorphosis was observed between BmTV- and mock-inoculated larvae. No virus RNA was detected in eggs laid by adults that had been inoculated with BmTV at larval stage. These findings demonstrate that BmTV is a novel rhabdovirus that persistently infects several lepidopteran cultured cells.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199548"},"PeriodicalIF":2.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and genetic characterization of Xiabuqu River virus: A novel member of the Iflaviridae family detected in soft ticks from Tibet, China","authors":"Meixi Ren ,&nbsp;Mingli Sun ,&nbsp;bataj Dan ,&nbsp;Yingxin Tu ,&nbsp;Guoyu Niu ,&nbsp;Weiping Lin ,&nbsp;Hengyi Sun ,&nbsp;Yujing Zhu","doi":"10.1016/j.virusres.2025.199543","DOIUrl":"10.1016/j.virusres.2025.199543","url":null,"abstract":"<div><div>Soft ticks, an important group of blood-sucking arthropods in nature, are widely distributed globally and can carry a wide range of pathogens, including Theileria ovis, Anaplasma ovis, Rickettsia spp<em>.</em> and African swine fever virus. In this study, we identified a novel single positive-stranded RNA virus, tentatively named Xiabuqu River virus (XRV), for the first time from soft ticks collected in Shigatse, Tibet. A total of 96 engorged soft ticks were collected from Tibetan sheep, with each tick assigned to a separate pool for analysis. The complete coding sequence of XRV was determined through next-generation sequencing, revealing a sequence length of 9277 nucleotides that includes a single open reading frame (ORF) encoding proteins such as the capsid protein, RNA helicase, and RNA-dependent RNA polymerase (RDRP). Quantitative RT-PCR and nested PCR were utilized to investigate the distribution of XRV within the tick sample. Pairwise distance analysis revealed that all obtained viral sequences shared a high nucleotide identity. Phylogenetic analysis demonstrated that XRV clustered with Lhasa Iflav tick virus 1, Fuyun tick virus 2, and Hubei tick virus 2. Further analyses indicated that XRV is a new member of the unclassified genus <em>Iflavirus</em> within the family <em>Iflaviridae</em>.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199543"},"PeriodicalIF":2.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meeting report: International soil virus conference 2024.","authors":"María Touceda-Suárez, Matthew A Perry, Riccardo Frizzo, John Henry Lotz-McMillen, Ruby Ann Gilmore, Shauna M Bennett, Jonelle T R Basso, William Donovan, Jane D Fudyma, Sara E Geonczy, Marissa Gittrich, Grant Gogul, Christina Hazard, Ellie Jameson, Lucie Jiraska, Sarah Stewart Johnson, James C Kosmopoulos, Ikaia Leleiwi, Bin Ma, Catherine M Mageeney, Andrew Millard, Uri Neri, Josué Rodríguez-Ramos, Simon Roux, Di Tong, Yiling Wang, Kurt Williamson, Ruonan Wu, Paula Dalcin Martins, Rumakanta Sapkota, Joanne B Emerson, Gareth Trubl","doi":"10.1016/j.virusres.2025.199544","DOIUrl":"10.1016/j.virusres.2025.199544","url":null,"abstract":"<p><p>The research field of soil viral ecology continues to advance rapidly as the roles of viruses in the functioning of soil ecosystems are increasingly recognized. To address recent developments in the field, the second International Soil Virus Conference was held in Livermore, California, USA, from June 25 to 27th, 2024, providing soil viral ecologists the opportunity to share new findings and suggest guidelines for future research, while encouraging international scientific discussion and collaboration. The meeting was held in person with sessions simultaneously streamed online. Fifty researchers attended from ten different countries and spanned a wide range of subfields and career stages. A total of 21 oral presentations were presented, followed by discussions covering key themes in soil viral research. This report summarizes the main takeaways and recommendations from the talks and discussions.</p>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":" ","pages":"199544"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening for HBV, HCV, TP and HIV in pregnant women from various ethnic groups in Yili, Xinjiang, China","authors":"Zhenzhen Pan ,&nbsp;Yuning Song ,&nbsp;Xiangyi Zhe ,&nbsp;Qin Zhang ,&nbsp;Shumei Yuan ,&nbsp;Zhe Zhao ,&nbsp;Hongwei Dong ,&nbsp;Jingru Hu ,&nbsp;Yu Zhao ,&nbsp;Guomei Zhang ,&nbsp;Zemin Pan ,&nbsp;Shaoqiang Zhang","doi":"10.1016/j.virusres.2025.199542","DOIUrl":"10.1016/j.virusres.2025.199542","url":null,"abstract":"<div><div>Analyze the infection status of four blood-borne infectious diseases, namely hepatitis B, hepatitis C, syphilis, and acquired immune deficiency syndrome (AIDS), among pregnant women from different ethnic groups in Yili, Xinjiang. The objective is to assess the prevalence of four infectious diseases among pregnant women in this region and provide reference for the prevention and elimination of mother-to-child transmission. Pregnant women of Han and Uygur ethnicity who underwent prenatal screening at our outpatient clinic between 2016 and 2022 were selected for screening for hepatitis B virus surface antigen (HBsAg), antibody to hepatitis C virus (anti-HCV), antibody to treponema pallidum (anti-TP) and antibody to human immunodeficiency virus (anti-HIV) using an enzyme-linked immunosorbent assay (ELISA). A total of 13,437 pregnant women were tested, aged between 18 and 47. The positive rate of four infectious disease markers in Han and Uygur pregnant women in this region was 6.97 % (936/13,437). The total positive rate of HBsAg was 6.44 % (865/13,437), among which the Han women of HBsAg positive rate was 6.63 % (836/12,608), and the HBsAg positive rate in Uygur women was 3.63 % (29/829) (χ2=12.673, <em>P</em> = 0.000); the total positive rate of anti-HCV was 0.16 % (21/13,437), of which the anti-HCV positive rate of Han women was 0.15 % (19/12,608), and anti-HCV positive rate in Uygur women was 0.24 % (2/829). (χ2=0.034, <em>P</em> = 0.853); the total positive rate of anti-TP was 0.34 % (46/13,437), of which the positive rate of anti-TP in Han women was 0.24 % (30/12,608), and the positive rate of anti-TP in Uygur women was 1.93 % (16/829) (χ2=65.280, <em>P</em> = 0.000); the total positive rate of anti-HIV was 0.03 % (4/13,437), of which Han nationality anti-HIV positive rate was 0 % (0/12,608), and the anti-HIV positive rate in Uygur nationality was 0.48 % (4/829) (<em>P</em> = 0.000). The positive rate of hepatitis B surface antibodies (anti-HBs) positive in Han nationality was 56.44 % (7116/12,608) and the positive rate of anti-HBs in Uygur nationality was 41.62 % (345/829) (χ2=65.219, <em>P</em> = 0.000); the all-negative detection rate of Han nationality was 29.04 % (3661/12,608) and the Uygur nationality of all-negative detection rate was 46.20 % (383/829) (χ2=104.352, <em>P</em> = 0.000). Our results suggest that the difference in infection rates between Han and Uygur pregnant women in Yili, Xinjiang, may be related to the different genetic susceptibility among different ethnic groups.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199542"},"PeriodicalIF":2.5,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the circadian rhythm signaling pathway in HTLV-1 pathogenesis using Boolean analysis","authors":"Abdollah Amiri ,&nbsp;Shayan Mardi ,&nbsp;Atefeh Bahavar ,&nbsp;Mohsen Sheikhi ,&nbsp;Somayeh Yaslianifard ,&nbsp;Sayed-Hamidreza Mozhgani","doi":"10.1016/j.virusres.2025.199539","DOIUrl":"10.1016/j.virusres.2025.199539","url":null,"abstract":"<div><div>The Human T-cell lymphotropic virus type 1 (HTLV-1), an oncogenic virus belonging to the Deltaretrovirus genus, expresses various proteins, including Tax and HBZ, which can affect many cellular pathways. In this study, we have investigated the role of the circadian rhythm signaling pathway, a key regulator of human health, in the pathogenesis of HTLV-1 using Boolean Network analysis and laboratory methods. After an extensive search of the circadian rhythm pathway, we analyzed the relationships between the genes of this pathway using the R programming language and the BoolNet package. Subsequently, we examined the impact of viral proteins on the cellular clock rhythm genes. Finally, we identified three genes, PER2, CRY1, and DEC1, as the main checkpoints from the attractors obtained. These three genes and two viral genes, Tax and HBZ, were quantitatively assessed on two groups of individuals, including ten asymptomatic carriers infected with HTLV-1 and ten healthy individuals using the qRT-PCR method. Our results showed that the expression level of PER2 and DEC1 genes was significantly higher in the asymptomatic carriers compared to the healthy control group. Also, we recorded positive correlations between PER2 and DEC1, CRY1 and DEC1, and negative correlations between HBZ and CRY1 and DEC1. In this study, we suggested that in asymptomatic carriers, the virus might try to induce a chronic infection by escaping from the immune system due to an alteration in circadian rhythm pathways. We also detected three promising genes in this pathway that could have therapeutic or diagnostic value in these individuals. However, this possibility requires further research in different periods, different groups (e.g., ATLL and HAM/TSP), and examining a more significant number of circadian rhythm genes.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"353 ","pages":"Article 199539"},"PeriodicalIF":2.5,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, characterization and genomic analysis of bacteriophages for biocontrol of vibriosis caused by Vibrio alginolyticus","authors":"Nattarika Chaichana ,&nbsp;Rutinan Rattanaburee ,&nbsp;Komwit Surachat ,&nbsp;Decha Sermwittayawong ,&nbsp;Natthawan Sermwittayawong","doi":"10.1016/j.virusres.2025.199529","DOIUrl":"10.1016/j.virusres.2025.199529","url":null,"abstract":"<div><div><em>Vibrio alginolyticus</em> is a significant opportunistic pathogen in marine environments, affecting both marine organisms and humans. The rise of antibiotic-resistant strains has prompted the exploration of bacteriophages as alternative biological control agents. In this study, 414 lytic bacteriophages specific to <em>V. alginolyticus</em> were isolated from various seafood and environmental samples. Phages P122, P125, and P160 demonstrated the broadest host range, effectively lysing 79.01 % of fish pathogenic <em>V. alginolyticus</em> strains and 44.69 % of environmental strains. However, no activity was observed against clinical <em>V. alginolyticus</em> strains or other tested species, including <em>V. harveyi, Escherichia coli, Staphylococcus aureus</em>, and <em>Aeromonas hydrophila</em>. One-step growth curve analysis revealed latent periods of 40 to 60 min and burst sizes ranging from 140 to 367 PFU/infected cells. Transmission electron microscopy (TEM) classified these phages within the class of Caudoviricetes with an icosahedral head and a long non-contractile tail. Moreover, whole-genome sequencing (WGS) identified genome sizes of approximately 76 kb, with 272–280 open reading frames (ORFs), no tRNA and pathogenic-associated genes. Comparative genomic analysis showed over 97 % similarity with other <em>Vibrio</em> phages. Phylogenetic analysis based on the terminase subunit also confirmed phages P122, P125, and P160 belonging to the class of Caudoviricetes. The phages were non-toxic to <em>Galleria mellonella</em> larvae and showed promise in reducing mortality rates when used as a cocktail treatment. The study highlights the potential of these phages as effective biocontrol agents in aquaculture, offering a promising alternative to antibiotics for managing <em>Vibrio</em> infections.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"353 ","pages":"Article 199529"},"PeriodicalIF":2.5,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}