Virus research最新文献

{"title":"Development of a pod pepper vein yellows virus-based expression vector for the production of heterologous protein or virus like particles in Nicotiana benthamiana","authors":"Lujia Wang ,&nbsp;Ge Zhang ,&nbsp;Shan Bu,&nbsp;Zina Lin,&nbsp;Jian Wu,&nbsp;Fei Yan,&nbsp;Jiejun Peng","doi":"10.1016/j.virusres.2025.199559","DOIUrl":"10.1016/j.virusres.2025.199559","url":null,"abstract":"<div><div>Plant viruses are emerging as a compelling alternative system for the heterologous production of pharmaceutical proteins, offering advantages in scalability, cost-effectiveness, and biological safety over traditional expression systems. They are increasingly recognized as effective platforms for biomedical applications, frequently used in the expression of human viral proteins and the display of peptides or proteins. The pod pepper vein yellows virus (PoPeVYV), classified within the genus <em>Polerovirus</em> of the <em>Solemoviridae</em> family, can substantially increase viral titers when co-infected with pod pepper vein yellows virus-associated RNA (PoPeVYVaRNA). This mixed infection methodology facilitates the formation of rod-shaped virus-like particles (VLPs), wherein modified green fluorescent protein (mGFP) is fused to the C-terminus of the coat protein (CP) from the pepper mild mottle virus (PMMoV). Notably, the expression of hepatitis B surface antigen (HBsAg) demonstrates a marked preference for plant viruses, allowing for enhanced expression via the PoPeVYV mixed infection system in <em>Nicotiana benthamiana</em>. Consequently, the PoPeVYV-based vector presents a promising alternative for the high-level production of heterologous proteins and rod-shaped VLPs in plants.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"355 ","pages":"Article 199559"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination therapy enhances the antiviral activity of IFN-λ against SARS-CoV-2 and MERS-CoV","authors":"Vahid Rajabali Zadeh ,&nbsp;Jocelyne M. Lew ,&nbsp;M. Atif Zahoor ,&nbsp;Deanna Santer ,&nbsp;Jordan J. Feld ,&nbsp;Darryl Falzarano","doi":"10.1016/j.virusres.2025.199560","DOIUrl":"10.1016/j.virusres.2025.199560","url":null,"abstract":"<div><div>Therapeutic options against pathogenic human coronaviruses remain limited. In a recent clinical trial, we demonstrated the therapeutic efficacy of pegylated-IFN-λ in COVID-19 outpatients. However, the emergence of variants that have the potential to evade IFN-mediated antiviral responses raises concerns regarding the continued efficacy of this approach. In this work, we compared the sensitivity of SARS-CoV-2 variants and MERS-CoV to IFN-λ treatment <em>in vitro</em> and explored the potential of combination therapy with other FDA-authorized or approved antiviral agents. We observed that in contrast to the ancestral strain, all other SARS-CoV-2 lineages showed varying, but increased resistance to IFN-λ treatment, from a 5.7-fold increase in EC₅₀ value for the P.1 strain to a 32.7-fold increase for the B.1.1.7 variant. We further show that combination treatment with remdesivir or nirmatrelvir enhanced the antiviral effect of IFN-λ against both SARS-CoV-2 and MERS-CoV. These findings justify the initiation of further <em>in vivo</em> testing that ultimately can help inform the development of more effective therapeutic guidelines against pathogenic coronaviruses.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"355 ","pages":"Article 199560"},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The epidemiological analysis of respiratory virus infections in Children in Hangzhou from 2019 to 2023","authors":"Xinfeng Zhao ,&nbsp;Xiaoxiao Zhu ,&nbsp;Jie Wang ,&nbsp;Cuiying Ye ,&nbsp;Shiyong Zhao","doi":"10.1016/j.virusres.2025.199558","DOIUrl":"10.1016/j.virusres.2025.199558","url":null,"abstract":"<div><div>To investigate the infection of children with respiratory tract infection in Hangzhou from 2019 to 2023, and to explore the epidemiological characteristics of respiratory tract virus infection before and after the prevalence of novel coronavirus. A retrospective analysis was conducted on oral and pharyngeal swabs from 302,680 children with acute respiratory infections. Colloidal gold-based assays were used to detect viral antigens, including adenovirus (ADV), influenza A (FluA), influenza B (FluB), and respiratory syncytial virus (RSV). The detection rates of different viruses were statistically analyzed, and comparisons were made regarding infection status before and after the COVID-19 pandemic, mixed infections, seasonal variations, and different age groups. Among the 302,680 samples, 65,493 tested positive for respiratory pathogens, resulting in a positive rate of 21.64 %. The highest positive rate was found for FluA single infections (12.77 %), while mixed infections were primarily observed with ADV combined with other respiratory viruses. There was a significant statistical difference in the overall detection rate of respiratory viruses before (2019), during (2020–2022), and after (2023) the COVID-19 pandemic (χ²=18,074.97, <em>P</em> &lt; 0.001). The positivity rates for FluA (χ²=31,866.75, <em>P</em> &lt; 0.001), FluB (χ²=255.407, <em>P</em> &lt; 0.001), RSV (χ²=338.76, <em>P</em> &lt; 0.001), and ADV (χ²=4110.093, <em>P</em> &lt; 0.001) also showed significant differences before, during, and after the pandemic. The epidemic seasons for FluA and FluB were winter and spring, while ADV did not exhibit a distinct seasonal pattern, and RSV had a peak incidence in winter. The highest positivity rate for ADV was observed in children aged 2–5 years (4.33 %), for FluA in the 5–15-year-old group (17.15 %), for FluB in those over 15 years (4.86 %), and for RSV in children aged 0–2 years (4.37 %). There were significant differences in virus infection positive rates across different age groups (χ²=2615.084, <em>P</em> &lt; 0.001). Additionally, the overall positive rate differed significantly by gender (χ²=87.317, <em>P</em> &lt; 0.001).The four common respiratory pathogens exhibit distinct epidemiological features in terms of age and seasonality. The COVID-19 pandemic has altered the epidemiology of respiratory viruses, particularly in terms of the peak seasons of infection. Clinically, attention should be given to the potential for co-infection with multiple pathogens.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"355 ","pages":"Article 199558"},"PeriodicalIF":2.5,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is it time to consider west Nile and Usutu viruses endemic in central Italy?","authors":"Federico Romiti ,&nbsp;Maria Teresa Scicluna ,&nbsp;Francesco Censi ,&nbsp;Florindo Micarelli ,&nbsp;Silvia Puccica ,&nbsp;Andrea Carvelli ,&nbsp;Marcello Giovanni Sala ,&nbsp;Irene Del Lesto ,&nbsp;Riccardo Casini ,&nbsp;Claudio De Liberato ,&nbsp;Silvia Tofani","doi":"10.1016/j.virusres.2025.199557","DOIUrl":"10.1016/j.virusres.2025.199557","url":null,"abstract":"<div><div>West Nile (WNV) and Usutu (USUV) viruses co-circulated in a region of Central Italy (Lazio) in 2018, as evidenced by the detection of WNV in the nervous tissues of symptomatic horses and USUV in blood donors and mosquito pools. To assess whether these viruses were endemic in the region, we analysed: 1) diapausing <em>Culex pipiens</em> mosquitoes collected during the winter seasons 2022–2023 and 2023–2024, 2) <em>Cx. pipiens</em> mosquitoes collected during the adult activity period from April to November in 2022 and 2023 across 4 provinces, and 3) sera from 52 horses and tissues from 537 birds. Field-collected <em>Cx. pipiens</em>, including both diapausing and non-diapausing individuals, were tested in pools for WNV and USUV using real-time RT-PCR. Serum samples from horses were tested with two WNV ELISA assays, IgM and IgG, while bird tissues were tested for both viruses via real-time RT-PCR. A total of 18,834 <em>Cx. pipiens</em> females were collected, including 9,812 mosquitoes during the winter seasons and 9,022 during the adult activity periods. Mosquitoes were tested in 623 pools, with all pools of diapausing mosquitoes testing negative for both viruses and 12 pools of non-diapausing mosquitoes positive to USUV. The WNV IgG positivity of 7 horse sera, which were negative at the beginning of the study period, was not confirmed by the virus neutralization test. All tissue samples were negative for WNV and USUV. Since WNV and USUV were not detected in diapausing mosquitoes, there was no evidence of the two viruses endemicity in the study area.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"355 ","pages":"Article 199557"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143621010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Systematic identification of HEV ORF2 interactome reveals that TMEM134 engages in ORF2-mediated NF-κB pathway” [Virus Research 228 (2017) 102–108]","authors":"Yabin Tian ,&nbsp;Weijin Huang ,&nbsp;Jun Yang ,&nbsp;Zhiheng Wen ,&nbsp;Yansheng Geng ,&nbsp;Chenyan Zhao ,&nbsp;Heqiu Zhang ,&nbsp;Youchun Wang","doi":"10.1016/j.virusres.2025.199553","DOIUrl":"10.1016/j.virusres.2025.199553","url":null,"abstract":"","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199553"},"PeriodicalIF":2.5,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of mycovirus composition in a hypovirulent strain of Sclerotinia sclerotiorum potentially uncovers mycovirus cross-taxa transmission","authors":"Lixia Gao ,&nbsp;Weimeng Li ,&nbsp;Jichun Jia ,&nbsp;Jiasen Cheng ,&nbsp;Yanping Fu ,&nbsp;Xueqiong Xiao ,&nbsp;Qing Cai ,&nbsp;Yang Lin ,&nbsp;Tao Chen ,&nbsp;Bo Li ,&nbsp;Xiao Yu ,&nbsp;Tom Hsiang ,&nbsp;Daohong Jiang ,&nbsp;Jiatao Xie","doi":"10.1016/j.virusres.2025.199552","DOIUrl":"10.1016/j.virusres.2025.199552","url":null,"abstract":"<div><div><em>Sclerotinia sclerotiorum</em> is a worldwide plant pathogenic fungus. Identifying novel mycoviruses in this fungus can aid in developing fungal disease control strategies and enhance our understanding of viral evolution. Here, we analyzed mycovirus composition in <em>S. sclerotiorum</em> strain XZ69, and identified six ssRNA mycoviruses, including five known mycoviruses and one unassigned mycovirus. The newly identified mycovirus, tentatively named Sclerotinia sclerotiorum narna-like virus 1 (SsNLV1/XZ69), possesses a full-length genome of 3534 nucleotides, containing a single ORF that encodes an RNA-dependent RNA polymerase (RdRp) of 1090 amino acids. The RdRp encoded by SsNLV1/XZ69 shares 60.4 % identity with that encoded by Monilinia narnavirus H. SsNLV1/XZ69 phylogenetically clusters with unclassified narna-like viruses potentially infecting fungi, plants, and animals, and they form an independent branch that is distant from established families, therefore supporting the establishment of a new family to accommodate these viruses. Sclerotinia sclerotiorum fusarivirus 3 (SsFV3/XZ69) share 97 % amino acid identities with preciously reported Botrytis cinerea fusarivirus 8 (BcFV8). This last mycovirus originated from <em>Botrytis cinerea</em>, and hence this reveals that cross-genus transmission of SsFV3 or BcFV8 between <em>B. cinerea</em> and <em>S. sclerotiorum</em> may have potentially occurred. Mycovirus elimination, horizontal transmission, and RNA transfection experiments revealed that Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV1/XZ69), SsNSRV2/XZ69, and SsFV3/XZ69 may be associated with hypovirulence in <em>S. sclerotiorum</em>, and strain XZ69 exhibits potential disease biocontrol on rapeseed seedlings. Our study expands our understanding of viral evolution, and may provide new potential biocontrol agents for <em>S. sclerotiorum</em>.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199552"},"PeriodicalIF":2.5,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-transcriptomic sequencing reveals divergent RNA viruses in geckos","authors":"Shuqi Liu ,&nbsp;Ruiling Niu ,&nbsp;Xinrui Wang ,&nbsp;Jingxuan Cui ,&nbsp;Mingxue Cui ,&nbsp;Hong Zhou ,&nbsp;Juan Li ,&nbsp;Edward C Holmes ,&nbsp;Weifeng Shi ,&nbsp;Cixiu Li","doi":"10.1016/j.virusres.2025.199551","DOIUrl":"10.1016/j.virusres.2025.199551","url":null,"abstract":"<div><div>Geckos are generally small, predominantly nocturnal reptiles, with several species commonly found close to human habitations. However, little is known about viral diversity in geckos. Using meta-transcriptomic sequencing we identified four novel RNA viruses – provisionally denoted Gecko astrovirus, Gecko parechovirus, Gecko reptillovirus, and Gecko hartmanivirus – in geckos sampled in October 2019 from Hainan Province, China. The presence of these viruses was confirmed by reverse transcription (RT)-PCR. Phylogenetic analyses revealed that these viruses were most closely related to those identified in various gecko species from China and Australia, such that they represent gecko-specific lineages, yet were also genetically distinct, with amino acid sequence identities to their closest relatives ranging from 38.6 % to 74.2 %. A co-phylogeny analysis revealed a complex interplay between long-term virus-host co-divergence and more recent host jumping, which differed in frequency among groups. In sum, we demonstrate the presence of four novel gecko-associated RNA viruses, expanding our understanding of viral diversity in these common animal species.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199551"},"PeriodicalIF":2.5,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-existing Anti-AAV9 antibodies in the Chinese healthy and rare disease populations: Implications for gene therapy","authors":"Qian Zhao ,&nbsp;Shuangqing Yu ,&nbsp;Diyi Fu ,&nbsp;Zhen Wu ,&nbsp;Jianfang Zhou ,&nbsp;Yi Yang ,&nbsp;Chen Chen ,&nbsp;Ni Wu ,&nbsp;Yucan Wang ,&nbsp;Wanlin Xi ,&nbsp;Ning Lou ,&nbsp;Xiaobing Wu ,&nbsp;Xiaohong Han","doi":"10.1016/j.virusres.2025.199549","DOIUrl":"10.1016/j.virusres.2025.199549","url":null,"abstract":"<div><div>The adeno-associated virus 9 (AAV9) vector was particularly notable for its broad tissue tropism, making it a preferred vector for gene therapy. Goals: The study aimed to investigate the patterns of pre-existing immunity against AAV9 in the Chinese population. In this study, we conducted a serological research from November 2022 to June 2024. The study included 341 participants in total with age ranged from 0 to 90 years old: 270 healthy individuals, 30 pediatric patients and 41 adults with rare diseases. Total AAV9-binding antibodies (TAbs) and neutralizing antibodies (NAbs) were measured. The seroprevalence of anti-AAV9 NAbs showed no significant differences between healthy individuals and rare disease patients across both pediatric and adult groups. Newborns exhibited a high NAb-positive rate (64.3 %), while children aged 6 months to 3 years had the lowest prevalence (7.7 %). This rate progressively increased through childhood and adolescence. Overall, 58.7 % of the Chinese population aged 0–90 years tested positive for anti-AAV9 NAbs, with adults showing a significantly higher prevalence than children (75.0 % vs. 34.3 %). Additionally, 58.1 % of the population exhibited low levels of anti-AAV9 NAb titers (IC₅₀ ≤ 100). No significant sex-specific differences were observed, and antibody titers (NAbs or TAbs) showed no strong correlation with age. A strong correlation was identified between TAb and NAb positivity rates and titers. The optimal AAV9-based GT period was between 6 months and 3 years in that patients possessed lowest pre-existing immunity. Since TAbs had a strong association with NAbs, TAbs was considered as an alternative indicator to screen rare diseases.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199549"},"PeriodicalIF":2.5,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adeno-associated virus 2 CRISPR/Cas9-mediated targeting of hepatitis B virus in tree shrews","authors":"Md Haroon Or Rashid ,&nbsp;Mohammad Enamul Hoque Kayesh ,&nbsp;Md Abul Hashem ,&nbsp;Tatsuro Hifumi ,&nbsp;Shintaro Ogawa ,&nbsp;Noriaki Miyoshi ,&nbsp;Yasuhito Tanaka ,&nbsp;Michinori Kohara ,&nbsp;Kyoko Tsukiyama-Kohara","doi":"10.1016/j.virusres.2025.199550","DOIUrl":"10.1016/j.virusres.2025.199550","url":null,"abstract":"<div><div>Chronic hepatitis B virus (HBV) infection is a global health issue with limited therapeutic options given the persistence of viral episomal DNA (cccDNA). Previously, we investigated the effects of adeno-associated virus 2 (AAV2) vector-mediated delivery of three guide (g)RNAs/Cas9 selected from 16 gRNAs. AAV2/WJ11-Cas9 effectively suppressed HBV replication <em>in vitro</em> and in humanized chimeric mouse livers. In the present study, we examined the effect of AAV2/WJ11-Cas9 on the acute phase of HBV genotype F infection in an immunocompetent northern tree shrew (<em>Tupaia belangeri</em>; hereafter, “tupaia”) model. AAV2/WJ11-Cas9 treatment significantly reduced the HBV viral load in serum at 1, 7, 10, and 14 days post-infection (dpi). HBV-F infection caused enlargement of hepatocytes and mild lymphocytic infiltration in the interlobular connective tissue. Thus, the virus damages hepatocytes and drives infection progression and HBV core antigen (HBcAg) accumulation, which were not observed in AAV2/WJ11-Cas9 treated and normal liver tissues. AAV2/WJ11-Cas9 treatment reduced HBV DNA and cccDNA in liver tissues, as well as serum levels of HBV surface antigen and HBV core-related antigen (HBcrAg), including HBcAg and HBeAg at 14 dpi. Anti-HBc, anti-HBs, and anti-AAV Abs production was also detected. AAV2/WJ11-Cas9 treatment suppressed inflammatory cytokines and <em>TLR1, TLR2, TLR3, TLR4, TLR6, TLR7</em>, and <em>TLR9</em> mRNA levels. Thus, WJ11/Cas9 delivered by AAV2 vectors may provide a new therapeutic approach for inhibiting HBV infection in immunocompetent animal models, which could be developed for use in humans through further translational research.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199550"},"PeriodicalIF":2.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel rhabdovirus persistently infects lepidopteran cell lines","authors":"Takeo Fukushima ,&nbsp;Shohei Takamura ,&nbsp;Keisuke Shoji ,&nbsp;Tomoyo Touguchi ,&nbsp;Susumu Katsuma ,&nbsp;Masashi Iwanaga","doi":"10.1016/j.virusres.2025.199548","DOIUrl":"10.1016/j.virusres.2025.199548","url":null,"abstract":"<div><div>A novel rhabdovirus was identified in BmN-4 (BmN) cells derived from <em>Bombyx mori</em> and Sf9 cells derived from <em>Spodoptera frugiperda</em>. Genome sequence homology revealed that this novel virus is distinct from the previously reported Spodoptera frugiperda rhabdovirus (SfRV). Given the high similarity of the <em>large protein</em> sequence of this virus to that of the Taarstrup virus, we named it Bombyx mori taarstrup virus (BmTV). When BmTV-negative <em>B. mori</em>-derived cultured cells were inoculated with BmTV, an increase in virus RNA was observed, but no impact on host cell proliferation occurred, suggesting that BmTV exhibits latent infection in insect cells. On the other hand, oral and hemocoel inoculation in <em>B. mor</em>i larvae did not cause significant increase in BmTV replication. After subcutaneous inoculation, no difference in adult metamorphosis was observed between BmTV- and mock-inoculated larvae. No virus RNA was detected in eggs laid by adults that had been inoculated with BmTV at larval stage. These findings demonstrate that BmTV is a novel rhabdovirus that persistently infects several lepidopteran cultured cells.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"354 ","pages":"Article 199548"},"PeriodicalIF":2.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}