Functional characteristics of endosymbiotic picornia-like virus in Diaphorina citri

IF 2.7 4区医学 Q3 VIROLOGY

Virus research Pub Date : 2025-08-16 DOI:10.1016/j.virusres.2025.199619

Shuai Wang , Muhammad Younas , Chengtao Bao , Yuxi Xiao , Inzamam Ul Haq , Jia Lü , Yu Bin , Taiyun Wei , You Li

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引用次数: 0

Abstract

The Asian citrus psyllid (Diaphorina citri) is the primary vector for Candidatus Liberibacter asiaticus (CLas), which is the causative agent of Huanglongbing (HLB). In recent years, increasing attention has been given to the endosymbiotic viruses harbored within D. citri, particularly Diaphorina citri picorna-like virus (DcPLV). However, the epidemiological distribution, biological effects, and role of DcPLV in pathogen transmission remain largely uncharacterized. This study investigates the field prevalence, transmission modes, and biological impacts of DcPLV in D. citri populations collected from 17 regions in China between 2023 and 2024. Detection of DcPLV revealed a widespread distribution, with an average infection rate of 35.18 %. Temporal monitoring in Gutian orchard further showed that DcPLV maintains consistently high infection levels throughout the year. The analysis of transmission showed that DcPLV is transmitted vertically from parents to offspring and horizontally from plant hosts. Biological assessments showed no significant effects of DcPLV on reproduction and adult longevity. Additionally, tissue-specific RT-qPCR revealed virus localization in the midgut and salivary glands, with viral accumulation progressively increasing during nymphal development and continuing into teneral adult. Furthermore, results demonstrated that DcPLV infection significantly enhanced CLas acquisition and accumulation in D. citri, which may contribute to increased vector competence. These findings provide novel insights on the interactions between DcPLV, the D. citri, and CLas.

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柑橘蚜内共生小冠状病毒的功能特征。

亚洲柑橘木虱（Diaphorina citri）是黄龙病（HLB）病原菌亚洲解放候选菌（Candidatus Liberibacter asiaticus, CLas）的主要传播媒介。近年来，柑桔内共生病毒的研究日益受到重视，尤其是柑桔小角样线虫病毒（dplv）。然而，dplv的流行病学分布、生物学效应以及在病原体传播中的作用在很大程度上仍未明确。本研究调查了2023 - 2024年在中国17个地区收集的柑橘种群中dplv的野外流行、传播方式和生物学影响。dplv的检测分布广泛，平均感染率为35.18%。古田果园的时间监测进一步表明，dplv全年保持较高的感染水平。传播分析表明，dplv通过亲本垂直传播给子代，通过植物寄主水平传播。生物学评估显示，dplv对生殖和成人寿命没有显著影响。此外，组织特异性RT-qPCR显示病毒定位于中肠和唾液腺，病毒积累在稚虫发育期间逐渐增加，并持续到一般成虫。结果表明，dplv感染显著增强了柑桔CLas的获取和积累，这可能有助于提高媒介能力。这些发现为dplv、D. citri和CLas之间的相互作用提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virus research 医学-病毒学

CiteScore

9.50

自引率

2.00%

发文量

239

审稿时长

43 days

期刊介绍： Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.