Virus research最新文献

{"title":"Isolation and characterization of lytic bacteriophage vB_KpnP_23: A promising antimicrobial candidate against carbapenem-resistant Klebsiella pneumoniae","authors":"Qian Wang ,&nbsp;Ran Chen ,&nbsp;Hui Liu ,&nbsp;Yue Liu ,&nbsp;Jinmei Li ,&nbsp;Yueling Wang ,&nbsp;Yan Jin ,&nbsp;Yuanyuan Bai ,&nbsp;Zhen Song ,&nbsp;Xinglun Lu ,&nbsp;Changyin Wang ,&nbsp;Yingying Hao","doi":"10.1016/j.virusres.2024.199473","DOIUrl":"10.1016/j.virusres.2024.199473","url":null,"abstract":"<div><div>The global health threat posed by carbapenem-resistant <em>Klebsiella pneumoniae</em> (CRKP) is exacerbated by the limited availability of effective treatments. Bacteriophages are promising alternatives to conventional antimicrobial agents. However, current phage databases are limited. Thus, identifying and characterizing new phages could provide biological options for the treatment of multi-drug resistant bacterial infections. Here, we report the characterization of a novel lytic phage, vB_KpnP_23, isolated from hospital sewage. This phage exhibited potent activity against carbapenemase-producing CRKP strains and was characterised by an icosahedral head, a retractable tail, and a genome comprising 40,987 base pairs, with a G + C content of 51 %. Capable of targeting and lysing nine different capsule types (K-types) of CRKP, including the clinically relevant ST11-K64, it demonstrated both high bacteriolytic efficiency and stability in various environmental contexts. Crucially, vB_KpnP_23 lacks virulence factors, antimicrobial resistance genes, or tRNA, aligning with the key criteria for therapeutic application. In vitro evaluation of phage-antibiotic combinations revealed a significant synergistic effect between vB_KpnP_23 and meropenem, levofloxacin, or amikacin. This synergy could lead to an 8-fold reduction in the minimum inhibitory concentration (MIC), suggesting that integrated treatments combining this phage with the aforementioned antibiotics may substantially enhance drug effectiveness. This approach not only extends the clinical utility of these antibiotics but also presents a strategic advance in combating antibiotic resistance. Specifically, it underscores the potential of phage-antibiotic combinations as a powerful tool in the treatment of infections caused by CRKP, offering a promising avenue to mitigate the public health challenges of antibiotic-resistant pathogens.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycoviruses from Aspergillus fungi involved in fermentation of dried bonito.","authors":"Seiji Buma, Syun-Ichi Urayama, Rei Suo, Shiro Itoi, Shigeru Okada, Akihiro Ninomiya","doi":"10.1016/j.virusres.2024.199470","DOIUrl":"10.1016/j.virusres.2024.199470","url":null,"abstract":"<p><p>Fungi are exploited for fermentation of foods such as cheese, Japanese sake, and soy sauce. However, the diversity of viruses that infect fungi involved in food fermentation is poorly understood. Fermented dried bonito (\"katsuobushi\") is one of the most important processed marine products in Japan. Fungi involved in katsuobushi fermentation are called katsuobushi molds, and Aspergillus spp. have been reported to be dominant on the surface of katsuobushi during fermentation. Because various mycoviruses have been found in members of the genus Aspergillus, we hypothesized that katsuobushi molds are also infected with mycoviruses. Here, we describe seven novel mycoviruses belonging to six families (Chrysoviridae, Fusariviridae, Mitoviridae, Partitiviridae, Polymycoviridae, and Pseudototiviridae) from isolated katsuobushi molds (Aspergillus chevalieri and A. sulphureus) detected by fragmented and primer-ligated double-stranded RNA sequencing. Aspergillus chevalieri fusarivirus 1 has a unique bi-segmented genome, whereas other known fusariviruses have a single genomic segment. Phenotypic comparison between the parental A. chevalieri strain infected with Aspergillus chevalieri polymycovirus 1 (AchPmV1) and isogenic AchPmV1-free isolates indicated that AchPmV1 inhibits the early growth of the host. This study reveals the diversity of mycoviruses that infect katsuobushi molds, and provides insight into the effect of mycoviruses on fungi involved in fermentation.</p>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into noncanonical small noncoding RNAs in Influenza A virus infection","authors":"Eun-A Ko ,&nbsp;Tong Zhou ,&nbsp;Jae-Hong Ko","doi":"10.1016/j.virusres.2024.199474","DOIUrl":"10.1016/j.virusres.2024.199474","url":null,"abstract":"<div><div>Influenza A virus (IAV) induces acute respiratory infections in birds and various mammals, including humans, and presents a significant global public health concern, with considerable economic consequences. Recently, researchers have shown keen interest in noncanonical small noncoding RNAs (sncRNAs) as carriers of epigenetic information, including tRNA-derived small RNAs (tsRNAs), rRNA-derived small RNA (rsRNAs), and Y RNA-derived small RNAs (ysRNAs). Particularly, tsRNAs and rsRNAs are detected in diverse species and demonstrate evolutionary conservation. We analyzed sncRNAs sequencing data in the pulmonary tissue of two genetically distinct mouse strains, C57BL/6J and DBA/2J, to explore strain-specific variations of sncRNAs in response to IAV infection. We systematically compiled information on noncanonical sncRNAs in these two strains and investigated the tsRNAs/rsRNAs/ysRNAs profiles influenced by IAV infection. Specifically, four noncanonical sncRNA families, including rsRNA-12S, GtsRNA-Arg-CCT, GtsRNA-Arg-TCT, and GtsRNA-Lys-TTT, exhibited upregulation upon IAV infection. Notably, DBA/2J mice showed earlier systemic differential expression of noncanonical sncRNAs after IAV infection compared to C57BL/6J mice. Additionally, our study revealed a strain-specific biogenesis of MtsRNAs in response to IAV infection. Also, distinct co-expression patterns of MtsRNAs were observed between C57BL/6J and DBA/2J mice, with DBA/2J mice showing broader positive co-expression of MtsRNAs with various sncRNA families compared to C57BL/6J mice. Our study provides a novel insight into noncanonical sncRNAs and their implications in IAV pathology and mouse strain specificity.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of SARS-CoV-2 variants during the XBB wave in the Republic of Korea","authors":"Jin Sun No ,&nbsp;Ji Yeong Noh ,&nbsp;Chae Young Lee ,&nbsp;Il-Hwan Kim ,&nbsp;Jeong-Ah Kim ,&nbsp;Yu Jeong Ahn ,&nbsp;Hyeokjin Lee ,&nbsp;Jeong-Min Kim ,&nbsp;Nam-Joo Lee ,&nbsp;Dong-Wook Lee ,&nbsp;Jeong-Hoon Kwon ,&nbsp;JeeEun Rhee ,&nbsp;Eun-Jin Kim","doi":"10.1016/j.virusres.2024.199471","DOIUrl":"10.1016/j.virusres.2024.199471","url":null,"abstract":"<div><div>As COVID-19 has become endemic, SARS-CoV-2 variants are becoming increasingly diverse, underscoring the escalating importance of global genomic surveillance. This study analyzed 86,762 COVID-19 samples identified in the Republic of Korea from September 2022 to November 2023. The results revealed a consistent increase in the prevalence of the XBB variants following the dominance of BN.1, with various XBB sub-lineages co-circulating in the Republic of Korea. The overall nucleotide diversity (π) among the SARS-CoV-2 genomes was 0.00155. Evolutionary analysis revealed that the average time interval between the first detection and estimated date of the most recent common ancestor of Korean XBB sub-lineages was 47 d, suggesting that the novel variants were efficiently identified in the Korean surveillance system. The mutation rate was determined to be in the range of 5.6 × 10^–4 to 9.1 × 10^–4 substitutions/site/year. In conclusion, this study provides insights into the genetic diversity and evolutionary interpretation of the XBB sub-lineages during the XBB wave in the Republic of Korea, highlighting the importance of continued genomic surveillance for emerging variants.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and identification of linear B-cell epitopes on structural proteins of African Swine Fever Virus","authors":"Haiyan Lu,&nbsp;Junjun Shao,&nbsp;Wei Liu,&nbsp;Shandian Gao,&nbsp;Guangqing Zhou,&nbsp;Xiaoyu Ning,&nbsp;Haiyan Huang,&nbsp;Yijia Liu,&nbsp;Huiyun Chang","doi":"10.1016/j.virusres.2024.199465","DOIUrl":"10.1016/j.virusres.2024.199465","url":null,"abstract":"<div><div>This study aims to screen and identify linear B-cell epitopes on the structural proteins of African Swine Fever Virus (ASFV) to assist in the development of peptide-based vaccines. In experiments, 66 peptides of 12 structural proteins of ASFV were predicted as potential linear B-cell epitopes using bioinformatics tools and were designed; the potential epitope proteins carried the GST tag were expressed, purified, and subjected to antigenicity analysis with porcine antiserum against ASFV, and further identified based on their immunogenicity in mice. A total of 22 potential linear B-cell epitopes showed immunoreactivity and immunogenicity. Of these epitopes, 13 epitopes were firstly identified including 4 epitopes located in p72 (352–363, 416–434, 424–439, 496–530 aa), 3 epitopes located in pE248R (121–136, 138–169, 158–185 aa), and only one epitope of each protein of pH108R (33–46 aa), p17 (63–86 aa), pE120R (65–117 aa), pE199L (175–189 aa), p12 (36–56 aa) as well as pB438L (211–230 aa). Notably, the immunoreactivity of the epitopes from the 63-86 aa of p17 and the 65–117 aa of pE120R were the highest amongst identified epitopes, while the immunogenicity of epitopes from the 36–56 aa of p12, the 211–230 aa of pB438L, the 352–363 aa of p72 and the 63–86 aa of p17 were the best strong. The other 9 epitopes are partly overlapped with previous researches. These epitopes identified here will further enrich the database of ASFV epitope, as well as help to develop safe, effective epitope-based ASF vaccines and ASF diagnostic reagents.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic analysis linked fatal neurologic disease in leopards (Panthera pardus) to Asia-5 lineage of canine distemper virus in Nepal","authors":"Amir Sadaula ,&nbsp;Prajwol Manandhar ,&nbsp;Bijaya Kumar Shrestha ,&nbsp;Parbat Jung Thapa ,&nbsp;Suresh Nepali ,&nbsp;Janardan Dev Joshi ,&nbsp;Babu Ram Lamichhane ,&nbsp;Rachana Shah ,&nbsp;Madhu Chetri ,&nbsp;Kiran Raj Rijal ,&nbsp;Kamal Prasad Gairhe ,&nbsp;Naresh Subedi ,&nbsp;Chiranjibi Prasad Pokheral ,&nbsp;Roji Raut ,&nbsp;Purushottam Pandey ,&nbsp;Bikalpa Karki ,&nbsp;Gita Pandey","doi":"10.1016/j.virusres.2024.199463","DOIUrl":"10.1016/j.virusres.2024.199463","url":null,"abstract":"<div><div>Canine distemper virus (CDV) is responsible for a highly contagious and often fatal neurological disease that affects various carnivores, including domestic dogs. In Nepal, recent reports of CDV exposure and illness in leopards (<em>Panthera pardus</em>) have raised concerns about the transmission of the virus among domestic dogs and wild carnivores. To investigate the genetic lineage and spread of CDV, our study utilized archived post-mortem samples from four leopards that exhibited clinical signs suggestive of canine distemper infection. These leopards were rescued in the Palpa, Dolakha, Kathmandu, and Parbat districts. Our phylogenetic analysis revealed that the CDV strains circulating among the leopards belong to the Asia-5 lineage, which is also prevalent among dogs and wild carnivores in Nepal and neighboring India. The genetic relatedness between the leopard CDV sequences and those from both dogs and other carnivores within the Asia-5 lineage suggests that leopards in Nepal may have acquired the virus from multiple sources, potentially facilitated by their generalist dietary habits preying on dogs and even mesocarnivores. Furthermore, we inspected specific amino acid substitution in the hemagglutinin gene of leopard CDV, which also suggests possible transmission from both domestic dogs and non-canid hosts, although further research is needed to draw definitive conclusions. Given the vulnerable state of the leopard population in Nepal, already threatened by poaching and retaliatory killing, the emergence of CDV as a potential novel threat is deeply concerning. Comprehensive surveillance studies are essential to understand the dynamics of CDV spillover and to develop informed interventions. Urgent measures, including vaccination programs and effective control of the dog population, are needed to mitigate the impact of this disease and safeguard the future of Nepal's leopards and other wild carnivores.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of targeted whole genome sequencing approaches for Crimean-Congo haemorrhagic fever virus (CCHFV)","authors":"Jake D'Addiego ,&nbsp;Sonal Shah ,&nbsp;Ayşe Nur Pektaş ,&nbsp;Bi̇nnur Köksal Bağci ,&nbsp;Murtaza Öz ,&nbsp;Sasha Sebastianelli ,&nbsp;Nazif Elaldı ,&nbsp;David J Allen ,&nbsp;Roger Hewson","doi":"10.1016/j.virusres.2024.199464","DOIUrl":"10.1016/j.virusres.2024.199464","url":null,"abstract":"<div><div>Crimean-Congo haemorrhagic fever (CCHF) is the most prevalent human tick-borne viral disease, with a reported case fatality rate of 30 % or higher. The virus contains a tri-segmented, negative-sense RNA genome consisting of the small (S), medium (M) and large (L) segments encoding respectively the nucleoprotein (NP), the glycoproteins precursor (GPC) and the viral RNA-dependent RNA polymerase (RDRP). CCHFV is one of the most genetically diverse arboviruses, with seven distinct lineages named after the region they were first reported in and based on S segment phylogenetic analysis.</div><div>Due to the high genetic divergence of the virus, a single targeted tiling PCR strategy to enrich for viral nucleic acids prior to sequencing is difficult to develop, and previously we have developed and validated a tiling PCR enrichment method for the Europe 1 genetic lineage.</div><div>We have developed a targeted, probe hybridisation capture method and validated its performance on clinical as well as cell-cultured material of CCHFV from different genetic lineages, including Europe 1, Europe 2, Africa 2 and Africa 3. The method produced over 95 % reference coverages with at least 10x sequencing depth. While we were only able to recover a single complete genome sequence from the tested Europe 1 clinical samples with the capture hybridisation protocol, the data provides evidence of its applicability to different CCHFV genetic lineages.</div><div>CCHFV is an important tick-borne human pathogen with wide geographical distribution. Environmental as well as anthropogenic factors are causing increased CCHFV transmission. Development of strategies to recover CCHFV sequences from genetically diverse lineages of the virus is of paramount importance to monitor the presence of the virus in new areas, and in public health responses for CCHFV molecular surveillance to rapidly detect, diagnose and characterise currently circulating strains.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001576/pdfft?md5=0befa523815397549149ae9521981693&pid=1-s2.0-S0168170224001576-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and genetic diversity of Parechovirus","authors":"Jia-Hao Zheng ,&nbsp;Zhi-Jian Zhou ,&nbsp;Zheng-Chan Liao ,&nbsp;Ye Qiu ,&nbsp;Xing-Yi Ge ,&nbsp;Xun Huang","doi":"10.1016/j.virusres.2024.199461","DOIUrl":"10.1016/j.virusres.2024.199461","url":null,"abstract":"<div><p>Human parechovirus (HPeV) is a common virus that can cause severe infections in newborns. Due to the limited knowledge of the prevalence of HPeV in different cities in China and the unknown association between HPeV infection and clinical characteristics of newborns, this research investigated the epidemiological and clinical characteristics of HPeV infection in hospitalized neonates in Changsha. From August to October 2023, 145 anal swab samples from 96 newborns and 38 pharyngeal swab samples from 33 newborns in the neonatal intensive care unit (NICU) were collected. The prevalence of HPeV was detected by reverse transcription-polymerase chain reaction (RT-PCR). The genomes of HPeV were sequenced and the viral protein 1 (VP1) region was used for genotyping. Phylogenetic analysis and recombination analysis of HPeV genome were performed. Finally, HPeV was detected in 10 out of 44 patients in October, all of them were HPeV-1. The sequenced 4 genomes of HPeV showed high genetic diversity with known strains. Additionally, a HPeV-1 recombinant strain was detected. Compared with HPeV negative patients, HPeV patients had higher prevalence of abdominal pain and diarrhea, intracranial hemorrhage, and purulent meningitis. Compared with HPeV negative patients, HPeV patients had higher peripheral blood lymphocytes, albumin, globulin, pH and lower peripheral blood neutrophils and hemoglobin. HPeV is an important viral cause of newborn infections and appears to be increasing in prevalence in recent years. Characteristic clinical pictures exist in HPeV infections, and further research is needed to accumulate more cases to obtain a comprehensive understanding of HPeV infections.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001540/pdfft?md5=b494f505e3990f48bb40a8798443ee6e&pid=1-s2.0-S0168170224001540-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Whole genome molecular analysis of respiratory syncytial virus pre and during the COVID-19 pandemic in Free State province, South Africa” [Virus Research, Volume 347, September 2024, 199421]","authors":"Hlengiwe Sondlane ,&nbsp;Ayodeji Ogunbayo ,&nbsp;Celeste Donato ,&nbsp;Milton Mogotsi ,&nbsp;Mathew Esona ,&nbsp;Ute Hallbauer ,&nbsp;Phillip Bester ,&nbsp;Dominique Goedhals ,&nbsp;Martin Nyaga","doi":"10.1016/j.virusres.2024.199449","DOIUrl":"10.1016/j.virusres.2024.199449","url":null,"abstract":"<div><div>Respiratory syncytial virus (RSV) is the most predominant viral pathogen worldwide in children with lower respiratory tract infections. The coronavirus disease 2019 (COVID-19) pandemic and resulting non-pharmaceutical interventions perturbed the transmission pattern of respiratory pathogens in South Africa. A seasonality shift and RSV resurgence was observed in 2020 and 2021, with several infected children observed.</div><div>Conventional RSV-positive nasopharyngeal swabs were collected from various hospitals in the Free State province, Bloemfontein, South Africa, from children suffering from respiratory distress and severe acute respiratory infection between 2020 to 2021. Overlapping genome fragments were amplified and complete genomes were sequenced using the Illumina MiSeq platform. Maximum likelihood phylogenetic and evolutionary analysis were performed on both RSV-A/-B G-genes with published reference sequences from GISAID and GenBank. Our study strains belonged to the RSV-A GA2.3.2 and RSV-B GB5.0.5a clades. The upsurge of RSV was due to pre-existing strains that predominated in South Africa and circulating globally also driving these off-season RSV outbreaks during the COVID-19 pandemic. The variants responsible for the resurgence were phylogenetically related to pre-pandemic strains and could have contributed to the immune debt resulting from pandemic imposed restrictions. The deviation of the RSV season from the usual pattern affected by the COVID-19 pandemic highlights the need for ongoing genomic surveillance and the identification of genetic variants to prevent unforeseen outbreaks in the future.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001424/pdfft?md5=257099debe7b71b54f3e0e68a6b619f2&pid=1-s2.0-S0168170224001424-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into RNA mycoviruses of fungal pathogens causing Fusarium head blight","authors":"Živilė Buivydaitė ,&nbsp;Anne Winding ,&nbsp;Lise Nistrup Jørgensen ,&nbsp;Athanasios Zervas ,&nbsp;Rumakanta Sapkota","doi":"10.1016/j.virusres.2024.199462","DOIUrl":"10.1016/j.virusres.2024.199462","url":null,"abstract":"<div><p>Fusarium head blight (FHB) continues to be a major problem in wheat production and is considered a disease complex caused by several fungal pathogens including <em>Fusarium culmorum, F. graminearum</em> and <em>F. equiseti</em>. With the objective of investigating diversity of mycoviruses in FHB-associated pathogens, we isolated <em>Fusarium</em> spp. from six wheat (<em>Triticum aestivum</em>) cultivars. In total, 56 <em>Fusarium</em> isolates (29 <em>F. culmorum</em>, 24 <em>F. graminearum</em>, one <em>F. equiseti</em>) were screened for mycoviruses by extracting and sequencing double-stranded RNA. We found that a large proportion of <em>Fusarium</em> isolates (46 %) were infected with mycoviruses. <em>F. culmorum</em>, previously described to harbor only one mycovirus, tended to host more viruses than <em>F. graminearum</em>, with a few isolates harboring seven mycoviruses simultaneously. Based on the RNA-dependent RNA polymerase domain analysis, ten were positive-sense single-stranded RNA viruses (related to viruses from families <em>Mitoviridae, Botourmiaviridae, Narnaviridae, Tymoviridae, Gammaflexiviridae</em>, as well as proposed Ambiguiviridae and ormycovirus viral group), one was double-stranded RNA virus (<em>Partitiviridae</em>), and five were negative-sense single-stranded RNA viruses (related to members in the families of <em>Yueviridae, Phenuiviridae, Mymonaviridae</em>, as well as proposed Mycoaspiviridae). Five mycoviruses were shared between <em>F. graminearum</em> and <em>F. culmorum</em>. These results increase our general understanding of mycovirology. To our knowledge, this is the first in-depth report of the mycovirome in <em>F. culmorum</em> and the first report on the diversity of mycoviruses from Danish isolates of FHB-causing fungi in general.</p></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168170224001552/pdfft?md5=6a7189badf7673728b7d9d824120ffbb&pid=1-s2.0-S0168170224001552-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142228966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}