从黑莓萎黄环斑病毒中提取的传染性克隆和基因沉默载体的开发与应用。

IF 2.5 4区医学 Q3 VIROLOGY

Virus research Pub Date : 2024-09-02 DOI:10.1016/j.virusres.2024.199460

Andrea Sierra-Mejia, Dan E V Villamor, Ioannis E Tzanetakis

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引用次数: 0

摘要

黑莓萎黄环斑病毒（BCRV）大约在 20 年前被描述出来，此后有多篇关于该病毒感染玫瑰科寄主（包括黑莓、覆盆子、玫瑰和苹果）的文章发表，感染率很高。但人们对该病毒对病害发展的影响仍然知之甚少。为了弥补这一知识空白，我们开发了一种 BCRV 感染克隆和病毒诱导基因沉默载体（VIGS）。感染性克隆可诱导全身感染，并通过机械传播评估重组病毒的可传播性。病毒诱导基因沉默载体使用了两种不同的插入物，证明了该构建体的多功能性。这项工作的成果可用于研究疾病的发展和控制，以及 BCRV 宿主的功能基因组学研究。

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Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus.

Blackberry chlorotic ringspot virus (BCRV) was described about 20 years ago and since then there have been several publications of the virus infecting rosaceous hosts including blackberry, raspberry, rose and apple at high rates. Still the effect of the virus on disease development is poorly understood. Aiming to bridge this knowledge gap, we developed a BCRV infectious clone and virus-induced gene silencing vector (VIGS). The infectious clone can induce systemic infection with the transmissibility of the recombinant virus evaluated through mechanical transmission. The VIGS induced silencing using two different inserts, proving the versatility of the construct. The products of this work can be used to study disease development and control as well as functional genomics studies of BCRV hosts.

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来源期刊

Virus research 医学-病毒学

CiteScore

9.50

自引率

2.00%

发文量

239

审稿时长

43 days

期刊介绍： Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.