高通量筛查丹麦猪体内的呼吸道病原体；分析循环猪呼吸道冠状病毒及其与其他病原体的关联。

IF 2.5 4区医学 Q3 VIROLOGY

Virus research Pub Date : 2024-11-26 DOI:10.1016/j.virusres.2024.199501

Amalie Ehlers Bedsted , Nicole B. Goecke , Charlotte K. Hjulsager , Pia Ryt-Hansen , Kasama Chusang Larsen , Thomas Bruun Rasmussen , Anette Bøtner , Lars E. Larsen , Graham J. Belsham

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引用次数: 0

摘要

猪呼吸道冠状病毒（PRCV）通常会引起猪的亚临床或轻微呼吸道感染，但也可能因其他因素而导致更严重的疾病。丹麦于 1984 年首次检测到 PRCV 感染，但缺乏有关其目前流行程度和多样性的数据。最近在丹麦约 75% 的猪血清中检测到了 PRCV 抗体。此外，还使用高通量 RT-qPCR 系统对猪鼻拭子样本进行了 PRCV 和其他 12 种呼吸道病原体的筛查。所有目标病原体都被检测到，但流行率不同。结果发现，PRCV 和某些其他病原体之间存在显著关联。从 PRCV 阳性样本中确定了部分尖峰基因序列和完整的核壳编码序列。在系统进化分析中，这些PRCV与早期的欧洲PRCV聚类，并与传染性肠胃炎病毒截然不同。我们的结论是，PRCV 在丹麦的猪群中广泛存在。有必要对 PRCV 的重要性进行进一步研究。

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High-throughput screening for respiratory pathogens within pigs in Denmark; analysis of circulating porcine respiratory coronaviruses and their association with other pathogens

Porcine respiratory coronavirus (PRCV) typically causes subclinical or mild respiratory infections in pigs, but may lead to more severe disease with other factors. PRCV infection in Denmark was initially detected in 1984, but data are lacking about its current prevalence and diversity. Antibodies against PRCV were detected in about 75 % of recent pig sera from Denmark. In addition, pig nasal swab samples were screened for PRCV and 12 other respiratory pathogens using a high-throughput RT-qPCR system. All targeted pathogens were detected but at different prevalences. Significant associations were found between the presence of PRCV and certain other pathogens. From PRCV positive samples, partial spike gene sequences and complete nucleocapsid coding sequences were determined. In phylogenetic analyses, these PRCVs clustered with earlier European PRCVs and were distinct from transmissible gastroenteritis virus. We conclude that PRCV is widespread within the pig population in Denmark. Further studies on the significance of PRCV are warranted.

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来源期刊

Virus research 医学-病毒学

CiteScore

9.50

自引率

2.00%

发文量

239

审稿时长

43 days

期刊介绍： Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.