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Morpho-Anatomy and Mathematical Modelling in Lilium philippinense Baker from Cordillera Central Range, Philippines. 菲律宾中部山脉菲律宾百合的形态解剖学和数学建模。
IF 1.4
Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.12
Jennifer C Paltiyan-Bugtong, Rey G Lumpio, Jones T Napaldet
{"title":"Morpho-Anatomy and Mathematical Modelling in <i>Lilium philippinense</i> Baker from Cordillera Central Range, Philippines.","authors":"Jennifer C Paltiyan-Bugtong,&nbsp;Rey G Lumpio,&nbsp;Jones T Napaldet","doi":"10.21315/tlsr2023.34.3.12","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.12","url":null,"abstract":"<p><p>The study presents the morphology, anatomy and mathematical modelling in Benguet lily (<i>Lilium philippinense Baker</i>), a threatened species from the Cordillera Central Range and was often misidentified with the weedy <i>L. formosanum</i>. The plant is an annual herb with linear, spiral leaves; pure white, perfect, funnel-shaped, showy flowers; septicidal elongated capsule; and, brown, light, winged seeds. New findings in the study include the description of the capsule and seeds, biometric measurements of the different plant organs, the significant correlation and regression model of plant height and stem diameter for certain floral measurement, and its diagnostic characteristics vis-à-vis <i>L. formosanum</i>. Interesting findings on the taxon's anatomy show a cross between a typical monocot and a typical dicot anatomy. It has a bifacial leaf structure (a common dicot character) but its spongy layer is not as widely spaced like in dicot leaf. The stem has a distinct cortex and pith (a dicot character) but has a scattered vascular bundles (a monocot character). Lastly, some roots have a narrow pith at the centre of the stele (a monocot character) while some roots have metaxylem elements at the centermost structure (a dicot character). Further studies need to be conducted to determine the ecological significance of these features.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Notes on Two Rare Species of Brachyuran Crabs (Families Matutidae and Parthenopidae) From Indonesian Waters with New Distribution Records. 印尼海域Brachyunan螃蟹两个珍稀物种(Matutidae和Parthenopidae)的新分布记录。
IF 1.4
Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.10
Nisfa Hanim, Ali Suman, Duranta Diandria Kembaren, Dyah Perwitasariv, Yusli Wardiatno, Achmad Farajallah
{"title":"Notes on Two Rare Species of Brachyuran Crabs (Families Matutidae and Parthenopidae) From Indonesian Waters with New Distribution Records.","authors":"Nisfa Hanim,&nbsp;Ali Suman,&nbsp;Duranta Diandria Kembaren,&nbsp;Dyah Perwitasariv,&nbsp;Yusli Wardiatno,&nbsp;Achmad Farajallah","doi":"10.21315/tlsr2023.34.3.10","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.10","url":null,"abstract":"<p><p>Several species of brachyuran crabs in Indonesian waters have not been reported since almost 100 years ago. This research reports a brachyuran crab that is rarely found and one new record in Indonesian waters. This study was conducted in the waters of southern Aru Island and the Malacca Strait using trawls during a cruise held by the Research Institute for Marine Fisheries, Ministry of Marine Affairs and Fisheries, Indonesia.. Our findings were <i>Izanami reticulata</i>, a new record (family Matutidae) from southern Aru Island, and <i>Cryptopadia fornicata</i> (family Parthenopidae) from the Malacca Strait. The two locations are close to where the species were found in previous studies: the Arafura Sea, which is adjacent to the Aru Islands, and the Malacca Strait, which is adjacent to Borneo. It is suspected that the presence of these two species in Indonesia is due to their distribution through sea currents during the pelagic larval stage. This article also provides the specific habitat for both species in Indonesia, which was previously unknown. In addition, this article contributes to strengthening Indonesia as a mega-biodiversity country with an initial compilation of a database of Brachyura in its waters.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection and Characterisation of Endosymbiont Wolbachia (Rickettsiales: Anaplasmataceae) in Elaeidobius kamerunicus (Coleoptera: Curculionoidea), Pollinating Agent of Oil Palm, and Its Relationships between Populations. 油棕授粉剂卡氏Elaeidobius kamerunicus(鞘翅目:Curculinoidea)体内共生体沃尔巴克氏体(立克次体:无浆菌科)的检测、鉴定及其种群关系。
IF 1.4
Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.5
Mohd Nur Azad Rushidi, Muhammad Luqman Hakim Azhari, Salmah Yaakop, Izfa Riza Hazmi
{"title":"Detection and Characterisation of Endosymbiont <i>Wolbachia</i> (Rickettsiales: Anaplasmataceae) in <i>Elaeidobius kamerunicus</i> (Coleoptera: Curculionoidea), Pollinating Agent of Oil Palm, and Its Relationships between Populations.","authors":"Mohd Nur Azad Rushidi,&nbsp;Muhammad Luqman Hakim Azhari,&nbsp;Salmah Yaakop,&nbsp;Izfa Riza Hazmi","doi":"10.21315/tlsr2023.34.3.5","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.5","url":null,"abstract":"<p><p><i>Elaeidobius kamerunicus</i> is the most efficient pollinator of oil palm. <i>Wolbachia</i> is an endosymbiotic bacteria associated with <i>E. kamerunicus</i> that has a potential to affect the fecundity and fitness of the <i>E. kamerunicus</i>. Despite their importance, no studies have been conducted to investigate its prevalence in <i>E. kamerunicus</i>. The objectives of this study were to detect and characterise <i>Wolbachia</i> in <i>E. kamerunicus</i> and determine the phylogenetic relationship of <i>Wolbachia</i> strains that infect <i>E. kamerunicus</i> by using three genetic markers namely Filamenting temperature-sensitive mutant Z (<i>ftsZ</i>), Chaperonin folding protein (<i>groEL</i>), and Citrate Synthase Coding Gene (<i>gltA</i>). DNA was extracted from 210 individuals of <i>E. kamerunicus</i> and the <i>Wolbachia</i> infections were detected using the <i>wsp</i> marker. The infected samples (<i>n</i> = 25, 11.9%) were then sequenced using <i>ftsZ, gltA</i> and <i>groEL</i> markers for strain characterization. In this study, a combination of four markers was used to construct the phylogeny of <i>Wolbachia</i>. Similar topologies were shown in all trees; Neighbour-Joining (NJ), Maximum Parsimony (MP), and Bayesian Inference (BI), which showed the mixing of individuals that harbor <i>Wolbachia</i> between populations. Interestingly, <i>Wolbachia</i> on <i>E. kamerunicus</i> was claded together with the species <i>Drosophila simulans</i> under supergroup B. This is the first report of <i>Wolbachia</i> infecting <i>E. kamerunicus</i> which is very valuable and significant as one of the parameters to evaluate the quality of the <i>E. kamerunicus</i> population for sustaining its function as a great pollinator for oil palm.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Characterisation of Culturable Thermophilic Cyanobacteria from Perak Hot Springs and their Plant Growth Promoting Properties Effects on Rice Seedlings (Oryza sativa L.). 霹雳温泉可培养嗜热蓝细菌的分离鉴定及其对水稻幼苗生长促进特性的影响。
IF 1.4
Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.1
Clement Kiing Fook Wong, Tzu Yee Chong, Ji Tan, Wey Lim Wong
{"title":"Isolation and Characterisation of Culturable Thermophilic Cyanobacteria from Perak Hot Springs and their Plant Growth Promoting Properties Effects on Rice Seedlings (<i>Oryza sativa</i> L.).","authors":"Clement Kiing Fook Wong,&nbsp;Tzu Yee Chong,&nbsp;Ji Tan,&nbsp;Wey Lim Wong","doi":"10.21315/tlsr2023.34.3.1","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.1","url":null,"abstract":"<p><p>Malaysia is home to a number of hot springs that are rich in microbial diversity including the photosynthetic cyanobacteria. Although this microbial community has been characterised based on metagenomics approach, the culturable thermophilic isolates have not been isolated and characterised extensively. Compared to the mesophiles, information on plant growth promoting (PGP) properties of these thermophiles remain largely untapped. As the amount of arable land for microbial bioprospecting is decreasing due to extensive human activities, the search for alternative source for microbial strains with PGP properties is important for the development of potential biofertilisers. This study sought to isolate and characterise culturable cyanobacteria strains from two local hot springs - Sungai Klah (SK) and Lubuk Timah (LT) located in Perak using morphological and molecular methods. The IAA production from the axenic cultures were measured. The PGP properties were also measured by priming the rice seeds with cyanobacterial water extracts. A total of six strains were isolated from both hot springs. Strains LTM and LTW from LT were identified as Leptolyngbya sp. whereas strains SEM, SEH, STH and STM were identified as <i>Thermosynechococcus elongatus</i>. All six strains produced IAA ranged from 670.10 pg/μL to 2010 pg/μL. The water extracts were found to increase the seed amylase activity of the rice seeds from 5th day of germination (DAG) to 10th DAG. In general, the IAA production and increased seed amylase activity might have contributed in enhancing the longest root length, shoot length and root-to-shoot (RS) ratio. To conclude, the thermophilic cyanobacteria from hot springs can be further exploited as a novel source of PGP microbes for the development of biofertilsers.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphometrics and Structural Changes of "Terung Asam" Sarawak (Solanum lasiocarpum Dunal) During Growth and Development. 砂拉越“Terung Asam”生长发育过程中的形态计量学和结构变化。
IF 1.4
Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.2
Albert Ting Koon Soon, Phebe Ding, Shiamala Devi Ramaiya
{"title":"Morphometrics and Structural Changes of \"Terung Asam\" Sarawak (<i>Solanum lasiocarpum</i> Dunal) During Growth and Development.","authors":"Albert Ting Koon Soon,&nbsp;Phebe Ding,&nbsp;Shiamala Devi Ramaiya","doi":"10.21315/tlsr2023.34.3.2","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.2","url":null,"abstract":"<p><p>\"Terung asam\" Sarawak (<i>Solanum lasiocarpum</i> Dunal) is an underutilised fruit vegetable. Information on the fruit growth is very lacking. Thus, this study was conducted to characterise fruit growth pattern based on physical characters and cellular structures. Data were recorded weekly from fruit set until senescence. All the morphological growth of \"terung asam\" fruit exhibits a single sigmoid growth pattern that fitted well to logistic model. There are three distinct phases of growth, i.e., S1, S2 and S3. At S1, the size of fruit cells was small without intercellular spaces. As fruit grew to S2, cell size increased with distinct vascular tissues. By S3, fruit has achieved its maximum size with green peel turn to yellow and finally golden yellow at late S3. Cuticle and two types of trichomes formed the outer layer of fruit. The thickness of fruit exocarp increased while density of trichomes decreased as fruit developed.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vivo Anti-Vibrio Evaluation of Sponge-Associated Bacteria on the Survival Rate of Litopenaeus vannamei Infected with Pathogenic Vibrio Species. 海绵相关细菌对凡纳滨对虾感染病原性弧菌存活率的体内抗弧菌评价
IF 1.4
Tropical life sciences research Pub Date : 2023-06-01 Epub Date: 2023-07-21 DOI: 10.21315/tlsr2023.34.2.15
Jepri Agung Priyanto, Galuh Adhiyaksa Ashari, Munti Yuhana, Aris Tri Wahyudi
{"title":"<i>In Vivo</i> Anti-<i>Vibrio</i> Evaluation of Sponge-Associated Bacteria on the Survival Rate of <i>Litopenaeus vannamei</i> Infected with Pathogenic <i>Vibrio</i> Species.","authors":"Jepri Agung Priyanto, Galuh Adhiyaksa Ashari, Munti Yuhana, Aris Tri Wahyudi","doi":"10.21315/tlsr2023.34.2.15","DOIUrl":"10.21315/tlsr2023.34.2.15","url":null,"abstract":"<p><p>Sponge-associated bacteria are considered a rich source of bioactive compounds particularly to reduce the risk of <i>Vibrio harveyi</i> and <i>Vibrio parahaemolyticus</i> infection. The present study aimed to analyse the effectiveness of 19 isolates to control <i>Vibrio</i> infection <i>in vivo</i>. All 19 isolates displayed a non-pathogenic characteristic on shrimps (cell density of 10<sup>6</sup> cells/mL) as analysed using the pathogenicity test. The mortality caused by both <i>Vibrio</i> spp. on 50% of the shrimp population (LC<sub>50</sub> value) had a cell density of 10<sup>5</sup> cells/mL as determined using the proportion interval method. On the basis of the challenge test, all isolates improved the survival rate of infected shrimps in diverse effectivities up to 89%, which was nearly 30% higher than the infected control. Two isolates coded as D6.9, and P5.20 reduced shrimp mortality after infection with <i>Vibrio</i> spp. 16S rRNA-based identification showed these isolates were closely similar to different genera of <i>Bacillus</i> and <i>Staphylococcus</i>. The extract derived from the most prospective isolate, D6.9, was dominated by 1-hydroxy-6-(3-isopropenyl-cycloprop-1-enyl)-6-methyl-heptan-2-one, hexadecanoic acid, 4-epicyclomusalenone [(24S)-24-methyl-28-norcycloart-25-en-3-one], and 2,4-dimethyl acetoacetanilide. This observation suggested these isolates characterised by <i>in vivo</i> anti-<i>Vibrio</i> activity need to be further developed as biocontrol candidates.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10735267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43465391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Antimalarial Susceptibility of Plasmodium falciparum and Plasmodium berghei Isolates to Selected Antimalarial Agents, Column Chromatographic Subfractions of Glyphaea brevis Leaves Extract and FTIR and GCMS of SF8. 恶性疟原虫和伯氏疟原虫分离株对所选抗疟药物的体外抗疟敏感性、草甘膦叶提取物的柱色谱亚组分以及SF8的FTIR和GCNS
IF 1.4
Tropical life sciences research Pub Date : 2023-06-01 Epub Date: 2023-07-21 DOI: 10.21315/tlsr2023.34.2.14
Tayo Micheal Anjuwon, Joseph Olorunmola Ehinmidu, Kola Matthew Anigo, Dorcas Bolanle James
{"title":"<i>In Vitro</i> Antimalarial Susceptibility of <i>Plasmodium falciparum</i> and <i>Plasmodium berghei</i> Isolates to Selected Antimalarial Agents, Column Chromatographic Subfractions of <i>Glyphaea brevis</i> Leaves Extract and FTIR and GCMS of SF8.","authors":"Tayo Micheal Anjuwon, Joseph Olorunmola Ehinmidu, Kola Matthew Anigo, Dorcas Bolanle James","doi":"10.21315/tlsr2023.34.2.14","DOIUrl":"10.21315/tlsr2023.34.2.14","url":null,"abstract":"<p><p>Malaria still remains a life-threatening parasitic disease with universal targets set for control and elimination. This study aimed to evaluate the <i>in vitro</i> antimalarial susceptibility of <i>Plasmodium falciparum</i> isolates and <i>Plasmodium berghei</i> to selected antimalarial agents and column chromatographic subfractions of <i>Glyphaea brevis</i> leaves extract and FTIR and GCMS of SF8. Trager and Jensen as well as World Health Organisation (WHO) standardised <i>in vitro</i> micro-test system methods were used to determine susceptibility on the patients' blood samples; Column chromatographic procedure was carried out to obtain 11 pooled fractions; FTIR and GCMS were used to determine functional groups and phytochemicals respectively. <i>In vitro</i> anti-plasmodial activity against <i>P. falciparum</i> clinical isolates had IC<sub>50</sub> range of 1.03 μg/mL-7.63 μg/mL while their IC<sub>50</sub> against <i>P. berghei</i> ranges from 4.32 μg/mL-7.89 μg/mL. Subfraction 8 (SF8) had the least IC<sub>50</sub> of 4.32 μg/mL. The FTIR spectrum showed the presence of isoprenoid, alcohol, phenol, alkane, alkenes, ester, carboxylic acids, aromatics and nitro compounds while GCMS identified dodecanoic acid, methyl ester; carotol; hexadecanoic acid, methyl ester; 9-octadecenoic acid (Z)-, methyl ester (oleic acid); methyl stearate; heptadecanoic acid, 16-methyl-, methyl ester; all with their antimalarial reported activities. In conclusion, <i>G. brevis</i> has a great potential for drug development against malaria parasite since it inhibited schizont growth and possesses phytocompounds with antimalarial report.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10735266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43354825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolution of the Food Web in Bandon Bay, the Gulf of Thailand: Ten Years of the Blue Swimming Crab Stocking Programme. 泰国湾班顿湾食物网的演变:蓝色梭子蟹养殖计划的十年
IF 1.4
Tropical life sciences research Pub Date : 2023-06-01 Epub Date: 2023-07-21 DOI: 10.21315/tlsr2023.34.2.6
Amonsak Sawusdee, Sontaya Koolkalya, Thanitha Thapanand, Tuantong Jutagate
{"title":"Evolution of the Food Web in Bandon Bay, the Gulf of Thailand: Ten Years of the Blue Swimming Crab Stocking Programme.","authors":"Amonsak Sawusdee, Sontaya Koolkalya, Thanitha Thapanand, Tuantong Jutagate","doi":"10.21315/tlsr2023.34.2.6","DOIUrl":"10.21315/tlsr2023.34.2.6","url":null,"abstract":"<p><p>The ecosystem of Bandon Bay, in the Gulf of Thailand (GoT), has been impacted since 2007 by the continued stocking of larval blue swimming crab <i>Portunus pelagicus</i>, also called a crab bank. In this study, the food web in the Bay was modelled using Ecopath software to compare the trophic status, interaction and energy flow among the components in the system in 2007 and 2016 (i.e., before and 10 years after the crab bank intervention). The models were based on data collected from trawling. Twenty fish and shellfish components were used in the 2007 model, while 22 were used in the 2016 model. A significant increase in biomass was found in blue swimming crab, but biomass declined for other demersal fishes, cephalopods, and Penaeid shrimps. The production/biomass ratios of most components were higher in 2016 but the consumption/biomass ratios were relatively unchanged. The ecotrophic efficiency indicated that shellfishes were more exploited than fishes. Changes in most of the ecological indices revealed higher maturity and stability after 10 years of crab bank operation. The mixed trophic impact indicated bottom-up regulation, and that the increase of blue swimming crab negatively impacted only Mantis shrimp. Overall, the results indicate positive impacts of the crab bank intervention.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10735257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47437121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction of Novel Yeast Strains from Candida tropicalis KBKTI 10.5.1 and Saccharomyces cerevisiae DBY1 to Improve the Performance of Ethanol Production Using Lignocellulosic Hydrolysate. 热带假丝酵母KBKTI 10.5.1和酿酒酵母DBY1菌株的构建提高木质纤维素水解物生产乙醇的性能
IF 1.4
Tropical life sciences research Pub Date : 2023-06-01 Epub Date: 2023-07-21 DOI: 10.21315/tlsr2023.34.2.5
Jamaluddin, Eny Ida Riyanti, Nisa Rachmania Mubarik, Edy Listanto
{"title":"Construction of Novel Yeast Strains from <i>Candida tropicalis</i> KBKTI 10.5.1 and <i>Saccharomyces cerevisiae</i> DBY1 to Improve the Performance of Ethanol Production Using Lignocellulosic Hydrolysate.","authors":"Jamaluddin, Eny Ida Riyanti, Nisa Rachmania Mubarik, Edy Listanto","doi":"10.21315/tlsr2023.34.2.5","DOIUrl":"10.21315/tlsr2023.34.2.5","url":null,"abstract":"<p><p>Increased consumption of xylose-glucose and yeast tolerance to lignocellulosic hydrolysate are the keys to the success of second-generation bioethanol production. <i>Candida tropicalis</i> KBKTI 10.5.1 is a new isolated strain that has the ability to ferment xylose. In contrast to <i>Saccharomyces cerevisiae</i> DBY1 which only can produce ethanol from glucose fermentation. The research objective is the application of the genome shuffling method to increase the performance of ethanol production using lignocellulosic hydrolysate. Mutants were selected on xylose and glucose substrates separately and using random amplified polymorphic DNA (RAPD) analysis. The ethanol production using lignocellulosic hydrolysate by parents and mutants was evaluated using a batch fermentation system. Concentrations of ethanol, residual sugars, and by-products such as glycerol, lactate and acetate were measured using HPLC machine equipped with Hiplex H for carbohydrate column and a refraction index detector (RID). Ethanol produced by Fcs1 and Fcs4 mutants on acid hydrolysate increased by 26.58% and 24.17% from parent DBY1, by 14.94% and 21.84% from parent KBKTI 10.5.1. In contrast to the increase in ethanol production on alkaline hydrolysate, Fcs1 and Fcs4 mutants only experienced an increase in ethanol production by 1.35% from the parent KBKTI 10.5.1. Ethanol productivity by Fcs1 and Fcs4 mutants on acid hydrolysate reached 0.042 g/L/h and 0.044 g/L/h. The recombination of the genomes of different yeast species resulted in novel yeast strains that improved resistance performance and ethanol production on lignocellulosic hydrolysates.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10735269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47190520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Species Distribution Model for the Asian Tapir and Vegetation Characteristics of Batang Gadis National Park, North Sumatra, Indonesia. 亚洲貘的物种分布模型和印度尼西亚北苏门答腊巴塘加迪斯国家公园的植被特征。
IF 1.4
Tropical life sciences research Pub Date : 2023-06-01 Epub Date: 2023-07-21 DOI: 10.21315/tlsr2023.34.2.4
Wanda Kuswanda, Freddy Jontara Hutapea, Muhammad Hadi Saputra, Bobby Nopandry
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