Tropical life sciences research最新文献

Genetic Variation and Phylogenetic Analysis of Indonesian Sugarcane (Saccharum officinarum L.) based on Internal Transcribed Spacer (ITS-nrDNA). 基于ITS-nrDNA的印尼甘蔗遗传变异及系统发育分析

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.3

Ganies Riza Aristya, Thoriq Abdul Halim, Tiara Putria Judith, Rina Sri Kasiamdari, Janis Damaiyani, Heri Prabowo

{"title":"Genetic Variation and Phylogenetic Analysis of Indonesian Sugarcane (Saccharum officinarum L.) based on Internal Transcribed Spacer (ITS-nrDNA).","authors":"Ganies Riza Aristya, Thoriq Abdul Halim, Tiara Putria Judith, Rina Sri Kasiamdari, Janis Damaiyani, Heri Prabowo","doi":"10.21315/tlsr2026.37.1.3","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.3","url":null,"abstract":"Sugarcane (Saccharum officinarum L.) is a crucial agricultural crop in global sugar production. Over the past decade (2010-2019), sugarcane production in Indonesia has experienced annual fluctuations, reaching its peak in 2013 with 35.5 million tons and declining to 27.7 million tons in 2019. Concurrently, Indonesia's sugar production only met 38% of domestic demand, necessitating the development of superior sugarcane cultivars with high sucrose content and resistance to pests and diseases. The aim of this study was to identify and determine the relationship among three sugarcane cultivars in Indonesia using DNA barcoding and constructing a phylogenetic tree based on ITS nuclear ribosomal DNA sequences. The ITS region was amplified using the primers ITS1 and ITS4, yielding a final base pair length of 531 bp. The sequences were then analysed to construct a phylogenetic tree using Maximum-Likelihood (ML) and Bayesian Inference (BI) methods. Sequence analysis was also conducted to determine genetic distances, polymorphic sites, haplotype distributions, and Principal Coordinate Analysis (PCoA). Phylogenetic tree construction grouped all cultivars into three distinct clades for both methods, with all three cultivars placed in the same clade with a bootstrap value of 94 for ML and a posterior probability of 1 for BI. Haplotype distribution showed that cultivars POJ and Pringu belonged to the same group, and PCoA analysis indicated no separation based on geographic origin, with some compositions similar to those of other countries such as Mexico, Taiwan and China.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"49-65"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Endiandric Acid from Beilschmiedia lumutensis and Their Molecular Docking Study as α-amylase and α-glucosidase Inhibitors. lumutensis中新的端花酸及其α-淀粉酶和α-葡萄糖苷酶抑制剂的分子对接研究。

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.15

Nur Amirah Saad, Muhammad Solehin Abd Ghani, Mohammad Tasyriq Che Omar, Mohd Azlan Nafiah, Unang Supratman, Desi Harneti, Cécile Apel, Marc Litaudon, Azeana Zahari, Khalijah Awang, Mohamad Nurul Azmi

{"title":"New Endiandric Acid from Beilschmiedia lumutensis and Their Molecular Docking Study as α-amylase and α-glucosidase Inhibitors.","authors":"Nur Amirah Saad, Muhammad Solehin Abd Ghani, Mohammad Tasyriq Che Omar, Mohd Azlan Nafiah, Unang Supratman, Desi Harneti, Cécile Apel, Marc Litaudon, Azeana Zahari, Khalijah Awang, Mohamad Nurul Azmi","doi":"10.21315/tlsr2026.37.1.15","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.15","url":null,"abstract":"A preliminary study showed that an ethyl acetate extract of the bark from Beilschmiedia lumutensis (Lauraceae family) exhibited promising inhibition activities against α-amylase and α-glucosidase by in vitro assays. Subsequently, this extract revealed three new cyclic polyketides endiandric acids, namely lumutensic acid A-C (1-3). Their structures were elucidated by 1D and 2D NMR, FT-IR, HRESIMS spectroscopic data analysis and by comparison with literature data. The molecular docking study showed that lumutensic acid C (3) showed the highest binding affinity and stability with both enzymes, with strong hydrogen bonding and hydrophobic interactions, outperfoming the standard drug acarbose. These findings suggest that compound 3 could be a promising candidate for anti-hyperglycemic therapeutic development, providing further insight into the potential of B. lumutensis as a source of bioactive compounds.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"315-338"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Activity, LC-MS/MS Identification and In Silico Analysis of the Ethanol Extract of Beneng Taro Leaves (Xanthosoma undipes K. Koch) Grown in Three Different Locations. 三种不同产地贝能芋叶乙醇提取物的抗氧化活性、LC-MS/MS鉴定及硅晶硅分析

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.2

Diana Widiastuti, Rudi Dwi Hariyanto, Siska Elisahbet Sinaga, Wira Eka Putra, Sustiprijatno, Ifa Manzila, M Ace Suhendar, Triastinurmiatiningsih, Surya Diantina

{"title":"Antioxidant Activity, LC-MS/MS Identification and In Silico Analysis of the Ethanol Extract of Beneng Taro Leaves (Xanthosoma undipes K. Koch) Grown in Three Different Locations.","authors":"Diana Widiastuti, Rudi Dwi Hariyanto, Siska Elisahbet Sinaga, Wira Eka Putra, Sustiprijatno, Ifa Manzila, M Ace Suhendar, Triastinurmiatiningsih, Surya Diantina","doi":"10.21315/tlsr2026.37.1.2","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.2","url":null,"abstract":"Xanthosoma undipes K. Koch (Beneng taro) leaves are rich in bioactive compounds with reported antioxidant and anticancer potential. This study evaluated the antioxidant activity of ethanol extracts from Beneng taro leaves cultivated in three locations, identified active compounds using liquid chromatography-tandem mass spectrometry (LC-MS/MS), and assessed their anticancer potential through in silico analysis. Extraction was performed with 96% ethanol, followed by phytochemical screening, quantification of total flavonoids and phenolics, and antioxidant evaluation using the DPPH assay. LC-MS/MS identified several bioactive phenolic and flavonoid compounds, including quercetin, isorhamnetin, hispidulin, oleocanthal and cyclovalone, as key contributors to antioxidant activity. Leaves from high-altitude Cisarua (TB-1) had the highest flavonoid (94.49 ± 1.61 mg QE/g) and phenolic (97.35 ± 1.74 mg GAE/g) contents, with the strongest antioxidant activity (IC50 = 42.96 μg/mL). Drug-likeness screening indicated favourable pharmacokinetic properties for several compounds. Molecular docking revealed strong binding affinities of quercetin, isorhamnetin, hispidulin, cochliophilin A, cyclovalone and oleocanthal to Kirsten rat sarcoma viral oncogene homolog (KRAS), a key oncogenic protein regulating cell growth, division and mutation, suggesting potential anticancer effects. These findings indicate that cultivation at higher altitudes enhances bioactive compound levels and antioxidant potential. Future studies should isolate active compounds, validate their effects in vivo, optimise cultivation practices, and explore their development into functional foods or nutraceuticals.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"31-47"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coproantigen ELISA Effectively Detects Moderate-To-High Fasciola Egg Counts with Limited Sensitivity at Low Levels in Field Settings. Coproantigen ELISA有效检测中至高片形吸虫卵计数，在低水平的现场设置有限的灵敏度。

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.11

Naim Che-Kamaruddin, Lokman Hakim Idris, Nur Mahiza Md Isa

{"title":"Coproantigen ELISA Effectively Detects Moderate-To-High Fasciola Egg Counts with Limited Sensitivity at Low Levels in Field Settings.","authors":"Naim Che-Kamaruddin, Lokman Hakim Idris, Nur Mahiza Md Isa","doi":"10.21315/tlsr2026.37.1.11","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.11","url":null,"abstract":"Detection of Fasciola eggs in ruminant faecal samples typically occurs around 15 weeks post-infection during patent infections. The recent introduction of coproantigen ELISA (cELISA) enables rapid antemortem diagnosis of fasciolosis, highlighting the need to evaluate its diagnostic performance under the field conditions. This study aimed to assess the usability and effectiveness of a cELISA kit for detecting Fasciola gigantica infections in free-grazing cattle from an endemic area, and to explore the relationship between cELISA results and conventional faecal egg counts obtained via the Flukefinder® sedimentation method. A total of 92 faecal samples (46 positive and 46 negative by faecal egg count) were analysed. The cELISA detection limit was identified at 4.5 eggs per gram (epg), achieving 100% positivity above this threshold. A moderate, statistically significant positive correlation was observed between faecal egg counts and cELISA optical density (OD) values (Spearman's r = 0.716, p < 0.01). Additionally, an odds ratio of 1.96 (p < 0.01) indicated that the likelihood of higher coproantigen levels nearly doubles with each additional egg detected. These findings suggest that cELISA is a sensitive and practical tool for diagnosing active Fasciola infections with moderate to high egg burdens, complementing traditional faecal sedimentation tests. Together, these approaches improve field diagnostics, enable targeted strategies and support effective fasciolosis control in endemic settings.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"227-239"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147820670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term Distribution, Nesting Activity, Hatching Success and Environmental Influences of the Hawksbill Turtle in Peninsular Malaysia. Part 1: A Case Study from 2015-2019. 马来西亚半岛玳瑁的长期分布、筑巢活动、孵化成功及环境影响。第一部分：2015-2019年的案例研究。

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.1

Sarahaizad Mohd Salleh, Shahrul Anuar Mohd Sah, Saufi Affandi Talib, Siti Mastura Daud, Azizah Ibrahim

{"title":"Long-term Distribution, Nesting Activity, Hatching Success and Environmental Influences of the Hawksbill Turtle in Peninsular Malaysia. Part 1: A Case Study from 2015-2019.","authors":"Sarahaizad Mohd Salleh, Shahrul Anuar Mohd Sah, Saufi Affandi Talib, Siti Mastura Daud, Azizah Ibrahim","doi":"10.21315/tlsr2026.37.1.1","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.1","url":null,"abstract":"Hawksbills' (Eretmochelys imbricata) status in the state of Melaka is continuously being assessed due to Melaka being the only prominent location for hawksbills in Peninsular Malaysia. Over five years of nesting season, results of the survey revealed the cumulative nests collected the most were in Padang Kemunting and Kem Terendak with a range between 400 nests-700 nests yearly. A year-round nesting trend was observed, and the preferable peak period tends to occur between June to August almost every year. The clutch size ranged from 5 to 243 eggs over five years of the survey. An increase in temperature and rainfall amount will influence the increasing number of nests, while humidity and precipitation did not impact the nesting per month. This article presents updated nesting statistics for hawksbill turtles, showing an increase in annual nesting activity over the five-year period from 2015 to 2019 in Melaka, compared to the earlier years of 2013-2014. A greater comprehension of hawksbills' reproductive potentiality under environmental force will aid conservation and management aims.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"1-29"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomics of a Photo-Fermentative Bacterial Solution and Its Effect on the Growth And Yield of Mini Green Cos Lettuce. 一种光发酵细菌溶液的宏基因组学及其对迷你绿Cos生菜生长和产量的影响。

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.5

Nanthavut Niyomvong, Duanpen Wongsorn, Nittaya Pitiwittayakul

{"title":"Metagenomics of a Photo-Fermentative Bacterial Solution and Its Effect on the Growth And Yield of Mini Green Cos Lettuce.","authors":"Nanthavut Niyomvong, Duanpen Wongsorn, Nittaya Pitiwittayakul","doi":"10.21315/tlsr2026.37.1.5","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.5","url":null,"abstract":"Photosynthetic bacteria (PSB) are widely utilised in agriculture to enhance plant growth and crop quality by improving nutrient uptake and phytohormone production. This study aimed to analyse the metagenomic composition of a photo-fermentative bacterial solution derived from fermentation and assess its effects on the growth and yield of Mini Green Cos lettuce. Metagenomic analysis revealed that Bacteroidota (38%) was the most abundant phylum, followed by Proteobacteria (23%), Thermotogota (17%) and Firmicutes (15%). Within Proteobacteria, Alphaproteobacteria was dominant followed by Gammaproteobacteria. At the genus level, Petrimonas (22%), uncultured clones belonging to family Petrotogaceae (17%), Rhodopseudomonas (11%), Rubrivivax (6%), and an unidentified genus from Lentimicrobiaceae (4%) were the most prevalent. These findings highlight the microbial diversity of PSB solution, suggesting its potential role in plant growth promotion. A plant growth experiment was conducted using a Completely Randomised Design (CRD) with four treatments: control (T1), chemical fertiliser (T2), undiluted PSB solution (T3) and PSB solution diluted at a 1:1 ratio (T4), with 10 replicates per treatment. Among all treatments, lettuce irrigated with undiluted PSB solution (T3) exhibited the highest growth rate, yield and total chlorophyll content. However, its performance was not significantly different from that of the chemical fertiliser treatment (T2). These results suggest that PSB can effectively promote plant growth and yield, yielding results comparable to chemical fertilisers. Therefore, photo-fermentative bacterial solutions offer a sustainable and eco-friendly alternative to chemical fertilisers, supporting environmentally conscious agricultural practices.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"85-108"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytoconstituents Analysis and In Vitro Antiproliferative Activity of Abrus precatorius Leaves on Cancer Cells. 鸡胸草叶片植物成分分析及对癌细胞的体外抗增殖活性研究。

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.12

Wan Suriyani Wan Ibrahim, Siti Farhanah Mohd-Salleh, Harishini Rajaratinam, Nor Amalia Nazri, John Ogbaji Igoli, Nurkhalida Kamal, Norzila Ismail

{"title":"Phytoconstituents Analysis and In Vitro Antiproliferative Activity of Abrus precatorius Leaves on Cancer Cells.","authors":"Wan Suriyani Wan Ibrahim, Siti Farhanah Mohd-Salleh, Harishini Rajaratinam, Nor Amalia Nazri, John Ogbaji Igoli, Nurkhalida Kamal, Norzila Ismail","doi":"10.21315/tlsr2026.37.1.12","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.12","url":null,"abstract":"Traditionally in Malaysia, the leaves of Abrus precatorius, a flowering plant that belongs to the legume family, Fabaceae, are used to treat various ailments such as coughs, diarrhoea, wound healing and even as anticancer and antivirus remedy. The study aimed to identify the phytocompounds present in A. precatorius leaves using (1) different solvents and (2) two different extraction methods for each solvent. Additionally, we have also intended to investigate the anti-proliferative activity of those maceration-based extracts on selected cancer and normal cell lines. In this study, two extraction methods were used (maceration and Soxhlet extraction), using sequential solvents according to their polarity, starting with hexane, ethyl acetate and methanol. The phytocompounds were identified via the GC-MS technique. The findings reported that the ethyl acetate extract (obtained via Soxhlet extraction) contained the highest amount of terpenoids, while the methanol extract (obtained via maceration) contained the highest combination of terpenoids and phenolic compounds. The triple negative breast cancer cell line, MDA-MB-231 showed high sensitivity towards all A. precatorius leaf extracts (maceration-based). The hexane extract of A. precatorius leaves (maceration-based) showed the lowest IC50 on MDA-MB-231 cells at 80.75 ± 64.0 μg/mL. In conclusion, potential anticancer phytocompounds were identified in the A. precatorius leaves extracts such as 1-octacosanol, neophytadiene and 4-vinylphenol. All maceration-based extracts exhibited non-cytotoxicity effect on both normal breast cell MCF-10a and fibroblast cell NIH (3T3). The MDA-MB-231 cell line was the most sensitive towards all maceration-based extracts, particularly hexane-maceration based.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"241-271"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solvent-Dependent Efficacy of Moringa Oleifera Leaf Extracts on Methicillin-Resistant Staphylococcus Aureus: Assessments on Antibacterial and Antioxidant Properties. 辣木叶提取物对耐甲氧西林金黄色葡萄球菌的溶剂依赖性：抑菌和抗氧化性能评价。

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.13

Ilakhiya Paavai Arunasalam, Seri Narti Edayu Sarchio, Nur Liyana Daud, Suhaili Shamsi, Mohd Nasir Mohd Desa, Muhamad Hafiz Abd Rahim

{"title":"Solvent-Dependent Efficacy of Moringa Oleifera Leaf Extracts on Methicillin-Resistant Staphylococcus Aureus: Assessments on Antibacterial and Antioxidant Properties.","authors":"Ilakhiya Paavai Arunasalam, Seri Narti Edayu Sarchio, Nur Liyana Daud, Suhaili Shamsi, Mohd Nasir Mohd Desa, Muhamad Hafiz Abd Rahim","doi":"10.21315/tlsr2026.37.1.13","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.13","url":null,"abstract":"The global surge in antibiotic resistance, particularly methicillin-resistant Staphylococcus aureus (MRSA), presents a significant public health challenge and emphasises the necessity for alternative therapeutic strategies. Moringa oleifera is rich in bioactive phytochemicals with established antimicrobial properties, however, the impact of various extraction solvents on its efficacy against MRSA has not been thoroughly investigated. This study offers a direct comparison of ethanolic, methanolic and aqueous M. oleifera leaf extracts (MOLE) to identify the optimal solvent for enhancing antibacterial and antioxidant activities. MOLE was extracted through maceration, and antibacterial activity against MRSA was assessed using colony-forming unit (CFU) assays at concentrations of 50 mg/mL, 100 mg/mL and 200 mg/mL, with vancomycin serving as the positive control. Antioxidant capacity was quantified using the ferric reducing antioxidant power (FRAP) assay, while phytochemical profiles characterised via liquid chromatography-mass spectrometry (LC-MS). The ethanolic extract exhibited the highest bioactivity, achieving complete MRSA inhibition at 100 mg/mL and demonstrating efficacy statistically comparable to vancomycin at 50 mg/mL. It also showed significantly greater antioxidant capacity across all tested concentrations. LC-MS analysis linked this enhanced activity to a richer composition of flavonoids and phenolic acids in the ethanolic extract. In summary, ethanol emerged as the most effective solvent for extracting antibacterial and antioxidant compounds from M. oleifera leaves. The findings underscore the potential of ethanolic MOLE as a natural therapeutic candidate against MRSA, warranting further investigation in preclinical models.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"273-291"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population Genetics of the Moon Crab Matuta victor (Fabricius 1781) on Java Island, Indonesia. 印度尼西亚爪哇岛月蟹（Fabricius 1781）的种群遗传学。

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.9

Nur Ikhlas Syuhada, Achmad Farajallah, Ali Mashar

{"title":"Population Genetics of the Moon Crab Matuta victor (Fabricius 1781) on Java Island, Indonesia.","authors":"Nur Ikhlas Syuhada, Achmad Farajallah, Ali Mashar","doi":"10.21315/tlsr2026.37.1.9","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.9","url":null,"abstract":"Genetic diversity is crucial in assessing biodiversity and is increasingly being utilised in conservation and management. This study aimed to analyse the population genetics of Matuta victor on Java Island. A total of 91 specimens was collected from 10 localities: Ujung Kulon, Pangandaran, Carita, Tanjung Pasir, Subang, Bancar, Banyuates, Ambunten, Situbondo and Banyuwangi. We obtained 801 bp fragments of COI mitochondrial DNA defining 38 different haplotypes, namely, 12 shared and 26 unique haplotypes. H1 was the most frequent, existing in eight localities. The haplotype diversity (Hd) and nucleotide diversity (π) ranged from 0.667 to 1 and 0.003 to 0.022, respectively. The overall haplotype diversity was high (Hd = 0.921), indicating the presence of many haplotypes across populations, while the overall nucleotide diversity was low (π = 0.018), suggesting only small genetic differences at the nucleotide level. AMOVA analysis showed a low level of genetic differentiation among the 10 locations (FST = 0.266). Neutrality tests indicated the potential population expansion of M. victor in several locations in Java. These findings are expected to constitute a significant first step in designing targeted and sustainable conservation strategies for this species in its diverse coastal habitats in Indonesia.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"185-203"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Length-Weight Relationship, Size Distribution and Sexual Maturity of Climbing Perch, Anabas testudineus (Bloch, 1792) From Different Habitats in Malaysia. 马来西亚不同生境攀鲈的长重关系、大小分布和性成熟。

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Tropical life sciences research Pub Date : 2026-03-01 Epub Date: 2026-03-31 DOI: 10.21315/tlsr2026.37.1.8

Mohamad Jalilah, Victor Tosin Okomoda, Khor Waiho, Hidayah Manan, Hassan Anuar, Thumronk Amornsakun And, Musa Nadirah

{"title":"Length-Weight Relationship, Size Distribution and Sexual Maturity of Climbing Perch, Anabas testudineus (Bloch, 1792) From Different Habitats in Malaysia.","authors":"Mohamad Jalilah, Victor Tosin Okomoda, Khor Waiho, Hidayah Manan, Hassan Anuar, Thumronk Amornsakun And, Musa Nadirah","doi":"10.21315/tlsr2026.37.1.8","DOIUrl":"https://doi.org/10.21315/tlsr2026.37.1.8","url":null,"abstract":"The growth and reproductive performance of fish are influenced by ecological factors. This study investigated the biology of the climbing perch, Anabas testudineus, in relation to habitat. A total of 2,880 climbing perch were collected from three habitats (irrigation canal, swamp and paddy field) in Peninsular Malaysia during the dry and rainy seasons, and their biometric measurements (total length and body weight) and sexual maturity indices were measured. Two-way Classification Analysis (TCA) revealed significant sexual dimorphism in all morphometric parameters, significant habitat effects on fish size, and pronounced seasonal variation in environmental parameters, while condition factor and seasonal effects on morphometrics were not significant. Results showed that body size was largest in fish from irrigation canals during the rainy season, with males averaging 12.84 ± 1.17 cm in total length (32.38 ± 4.4 g body weight) and females averaging 13.70 ± 1.83 cm (52.84 ± 12.54 g), consistent with the upper size range reported for this species. Fish from irrigation canals also exhibited more advanced gonadal development, confirmed by higher gonadosomatic indices (GSI) and histological evidence of vitellogenic oocytes in females and spermatozoa-filled seminiferous lobules in males. Principal Component Analysis (PCA) indicated that larger body sizes and advanced gonadal development were associated with higher dissolved oxygen and pH, whereas smaller sizes correlated with elevated water temperature. This study highlights the critical roles of sex, habitat quality and seasonal variation in shaping the growth and reproductive traits of wild climbing perch populations and provides evidence-based insights for fisheries management in tropical freshwater ecosystems.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"37 1","pages":"157-184"},"PeriodicalIF":0.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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