Acute and Subacute Oral Toxicity Assessment of The Polysaccharides Extracted from Clinacanthus nutans Leaves: A Preclinical Model for Drug Safety Screening.

IF 1.1 Q3 BIOLOGY
Tropical life sciences research Pub Date : 2025-03-01 Epub Date: 2025-03-30 DOI:10.21315/tlsr2025.36.1.13
Tan Yong Chia, Chee-Yuen Gan, Gurjeet Kaur, Pike-See Cheah, Vikneswaran Murugaiyah, Ashfaq Ahmad, Bader Alsuwayt, Sulaiman Mohammed Abdullah Alnasser, Muhammad Hakimin Shafie, Selvamani Narayan Nair, Mohammed H Abdulla, Edward James Johns
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引用次数: 0

Abstract

Emerging investigations have indicated that many plant polysaccharides may be beneficial for treating metabolic diseases. To date, the therapeutic efficacy and potential toxicity of polysaccharides extracted from Clinacanthus nutans (C. nutans) remain unexplored. This study investigated the in vivo acute and subacute oral toxicological profiles of the highest doses of C. nutans bioactive polysaccharides (CNBP) extracted from the leaves using conventional toxicity methods. The total of 39 healthy 8-10 weeks male Sprague-Dawley rats (n = 3) were randomly assigned to control (C), acute (A) and subacute (SA) groups receiving 125, 250, 500, 1,000, 2,000 or 3,000 mg/kg/day of CNBP extract, respectively. The acute group received a single dose of CNBP extract, whereas the subacute group received daily single doses of CNBP extract for 14 days. Oral administration of up to 3,000 mg/kg CNBP extract caused no abnormal signs of toxicity during 14 days. However, daily administration of 500 mg/kg or higher doses of CNBP extract for 14 days induced a mild degree of toxicity in the liver, characterised by elevated alkaline phosphatase levels with C (163 ± 9 U/L) vs. SA500 (222 ± 49 U/L), SA1000 (223 ± 29 U/L), SA2000 (238 ± 33 U/L) and SA3000 (252 ± 18 U/L). CNBP extracts exhibit therapeutic potential, exemplified by diuretic, natriuretic, anti-hypertensive, anti-tachycardia, reno-protective and cholesterol-lowering properties. Precautions should be taken when administering the extracts at higher doses and for longer durations.

山棘叶多糖急性和亚急性口服毒性评价:药物安全性筛选的临床前模型
新的研究表明,许多植物多糖可能对治疗代谢性疾病有益。迄今为止,从山核桃中提取的多糖的治疗效果和潜在毒性尚未得到充分的研究。本研究采用常规毒理学方法,研究了高剂量山茱萸叶生物活性多糖(CNBP)的急性和亚急性口服毒理学特征。选取健康8-10周龄雄性Sprague-Dawley大鼠39只(n = 3),随机分为对照组(C)、急性组(A)和亚急性组(SA),分别给予125、250、500、1,000、2,000和3,000 mg/kg/d的CNBP提取物。急性组给予单剂量CNBP提取物,亚急性组给予每日单剂量CNBP提取物,连续14天。口服高达3,000 mg/kg的CNBP提取物在14天内没有引起异常的毒性迹象。然而,连续14天每天服用500 mg/kg或更高剂量的CNBP提取物可引起肝脏轻度毒性,其特征是碱性磷酸酶水平升高,C(163±9 U/L)高于SA500(222±49 U/L)、SA1000(223±29 U/L)、SA2000(238±33 U/L)和SA3000(252±18 U/L)。CNBP提取物具有利尿、利钠、抗高血压、抗心动过速、肾保护和降胆固醇等治疗潜力。当以较高剂量和较长时间使用提取物时,应采取预防措施。
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来源期刊
CiteScore
2.60
自引率
0.00%
发文量
40
审稿时长
20 weeks
期刊介绍: Tropical Life Sciences Research (TLSR) formerly known as Journal of Bioscience seeks to publish relevant ideas and knowledge addressing vital life sciences issues in the tropical region. The Journal’s scope is interdisciplinary in nature and covers any aspects related to issues on life sciences especially from the field of biochemistry, microbiology, biotechnology and animal, plant, environmental, biomedical and pharmaceutical sciences. TLSR practices double blind peer review system to ensure and maintain the good quality of articles published in this journal. Two issues are published annually in printed and electronic form. TLSR also accepts review articles, experimental papers and short communications. The Chief Editor would like to invite researchers to use this journal as a mean to rapidly promote their research findings.
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