Bioassay-Guided Fractionation of Acetone and Methanol Extracts of Quercus infectoria Galls with Antimalarial Properties.

IF 1.1 Q3 BIOLOGY

Tropical life sciences research Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI:10.21315/tlsr2024.35.2.8

Nurul Hammizah Hamidon, Anjana Chamilka Thuduhenage Dona, Nik Nor Imam Nik Mat Zin, Nurul Izza Nordin, Shaida Fariza Sulaiman, Nurhidanatasha Abu-Bakar

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Abstract

The antimalarial properties of crude extracts from Quercus infectoria galls were investigated through bioassay-guided fractionation. Acetone (QIA) and methanol (QIM) crude extracts have been reported to have promising antimalarial activity against Plasmodium falciparum (3D7 strain). These extracts were subjected to fractionation using automated preparative high-performance liquid chromatography (prep-HPLC) to identify the most active fractions. Nine fractions were isolated from each extract, of which the fractions QIA11 and QIM16 showed antimalarial activity, with IC₅₀ values of 17.65 ± 1.82 μg/mL and 24.21 ± 1.88 μg/mL, respectively. In comparison, the standard antimalarial drug artemisinin has an IC₅₀ value of 0.004 ± 0.001 μg/mL). Through high-resolution liquid chromatography coupled with mass spectrometry (HR-LCMS) analysis of the fractions, four known compounds were successfully identified: gallic acid, ellagic acid, 1,3,6-tris-o-(3,4,5-trihydroxybenzoyl)-beta-d-glucose and 1-O,6-O-digalloyl-beta-D-glucose.

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生物测定指导下丙酮和甲醇提取物的抗疟特性

通过生物测定指导下的分馏，研究了柞树虫瘿粗提取物的抗疟特性。据报道，丙酮（QIA）和甲醇（QIM）粗提取物对恶性疟原虫（3D7 株）具有良好的抗疟活性。这些提取物通过自动制备型高效液相色谱法（prep-HPLC）进行分馏，以确定最具活性的馏分。从每种提取物中分离出 9 个馏分，其中 QIA11 和 QIM16 显示出抗疟活性，IC50 值分别为 17.65 ± 1.82 μg/mL 和 24.21 ± 1.88 μg/mL。相比之下，标准抗疟药青蒿素的 IC50 值为 0.004 ± 0.001 μg/mL）。通过对馏分进行高分辨液相色谱-质谱（HR-LCMS）分析，成功鉴定出四种已知化合物：没食子酸、鞣花酸、1,3,6-三-邻（3,4,5-三羟基苯甲酰基）-beta-D-葡萄糖和 1-O,6-邻二甲酰-beta-D-葡萄糖。

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来源期刊

Tropical life sciences research BIOLOGY-

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Tropical Life Sciences Research (TLSR) formerly known as Journal of Bioscience seeks to publish relevant ideas and knowledge addressing vital life sciences issues in the tropical region. The Journal’s scope is interdisciplinary in nature and covers any aspects related to issues on life sciences especially from the field of biochemistry, microbiology, biotechnology and animal, plant, environmental, biomedical and pharmaceutical sciences. TLSR practices double blind peer review system to ensure and maintain the good quality of articles published in this journal. Two issues are published annually in printed and electronic form. TLSR also accepts review articles, experimental papers and short communications. The Chief Editor would like to invite researchers to use this journal as a mean to rapidly promote their research findings.