Tropical life sciences research最新文献

{"title":"Taxonomic Significance of Leaf Anatomical Characteristics of Selected <i>Globba</i> L. (Zingiberaceae) Species in Peninsular Malaysia.","authors":"Noraini Talip, Syazwani Basir, Che Nurul Aini Che Amri, Mohd Norfaizal Ghazalli, Ahmad Fitri Zohari, Muhammad Amirul Aiman Ahmad Juhari, Mohamad Ruzi Abdul Rahman, Hamidun Bunawan","doi":"10.21315/tlsr2025.36.2.3","DOIUrl":"10.21315/tlsr2025.36.2.3","url":null,"abstract":"<p><p>A comparative leaf anatomy study was carried out on 10 taxa of <i>Globba</i> L. (Zingiberaceae) in Peninsular Malaysia to ascertain their systematic significance, especially for species differentiation and identification. Methods used include leaf clearing, leaf sectioning using a sliding microtome and observation under a scanning electron microscope. Results showed that a combination of the following characters have taxonomic significance in <i>Globba</i> spp. Studied, i.e., outlines of leaf margin, midribs, petioles and their relative sizes; number of vascular bundles in arc I, II, III and IV in the midribs and petioles; absence or presence and type of trichomes; presence of fibre caps and girders in vascular bundles in the leaf lamina, as well as the presence of hypodermal layers below adaxial epidermis. Anatomical characteristics such as tufted trichomes in <i>G. patens</i>, papilose adaxial epidermal cells in <i>G. variabilis</i> var. <i>pusilla</i>, rounded margin in <i>G. variabilis</i> and an arc I vascular bundle present only in the midrib of <i>G. variabilis</i> var. <i>pusilla</i> can be used as diagnostic characters for each species. <i>Globba variabilis</i> var. <i>pusilla</i> which is closely related to <i>G. variabilis</i> can be distinguished by the papilose epidermal cells. This study proved that leaf epidermis anatomical characteristics could possibly be applied to the identification of some species in <i>Globba</i> with certainty.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"59-82"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Salinity Stress on Growth, Physiology and Anatomy of Pumpkin (<i>Cucurbita moschata</i> Duchesne) cv. Kang Kog.","authors":"Chaichan Maneerattanarungroj, Sununta Sudjai, Narisa Kunpratum, Pitakpong Maneerattanarungroj, Worasitikulya Taratima","doi":"10.21315/tlsr2025.36.2.7","DOIUrl":"10.21315/tlsr2025.36.2.7","url":null,"abstract":"<p><p>Pumpkin is an important economic crop with high nutritional value. Different pumpkin varieties experience diverse growth problems due to soil salinity. This research studied the physiological and anatomical adaptations of the Kang Kog pumpkin cultivar to salinity stress. Pumpkin seedlings were grown under a hydroponic system using Hoagland's solution with NaCl concentrations of 0 mM, 25 mM, 50 mM, 75 mM and 100 mM for four weeks. Results showed that pumpkin leaf number, leaf width, leaf length, root number, root length, plant height, stem diameter, fresh weight and dry weight significantly decreased after exposure to high NaCl concentrations. Chlorophyll a and green intensity measured as SPAD units also significantly decreased, while chlorophyll fluorescence (Fv/Fm, Fv'/Fm') and chlorophyll <i>b</i> content of all treated groups were not significantly different when compared to the control group. Fibre strands and cuticles in all treatments were significantly thicker compared to the control group, while vessel diameters and vascular bundle sizes of the treated groups significantly decreased compared to the control group. Results showed that salinity stress did not impact chlorophyll <i>b</i> and chlorophyll fluorescence. Kang Kog pumpkins can adapt and grow in slightly saline environments. Our results provide important information for pumpkin breeding programs efforts that can be used in combining with other agronomic characters to improve tolerant cultivars under initial salinity stress tolerance.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"141-158"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial Purification, Characterisation and Gene Detection of Protease by <i>Lacticaseibacillus paracasei</i> IN17 from Inasua.","authors":"Naurah Fikriani Zawawi, Nisa Rachmania Mubarik, Laksmi Ambarsari","doi":"10.21315/tlsr2025.36.2.8","DOIUrl":"10.21315/tlsr2025.36.2.8","url":null,"abstract":"<p><p>Enzymes are functional proteins that accelerate chemical reactions and reduce the activation energy. <i>Lacticaseibacillus paracasei</i>, a lactic acid bacterium, is widely used as a probiotic supplement in the food industry. <i>L. paracasei</i> strain IN17 was isolated from Inasua, Indonesian fish fermentation products. It produces an extracellular protease that hydrolyses peptide bonds to produce peptides or amino acids. It is important to characterise bacteria that produce protease to know their activity and potency. This study aims to purify, characterise and detect the protease-encoding gene from <i>L. paracasei</i>. Isolates that have been identified were tested qualitatively by measuring the proteolytic index. The optimal production was known for two incubation treatments with static and dynamic conditions. The crude extract was precipitated with ammonium sulfate in the concentration range of 0%-80% (w/v). Enzyme activity characterisation was carried out based on optimum pH and temperature, the effect of cation metal, inhibitor and protease kinetics. The static condition had higher growth and protease-specific activity than the dynamic condition, which were 0.137 U/mg and 0.054 U/mg, respectively, at 18 h of production. Ammonium sulfate saturation at a concentration of 20% (w/v) resulted in a protease purity 7.36. The optimal activity of crude extract and precipitated protease were at pH 7 and 6, respectively. Both crude extract and precipitated protease have an optimal temperature at 30°C, activated by Mn cofactor and inhibited by EDTA with >50% inhibition percentage. This protease represents a metalloprotease. The gene encoding protease (<i>prt</i>P) was successfully amplified with ~685 bp visualised in an agarose gel 1% (w/v).</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"159-177"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Light Emitting Diodes (LEDs) Spectra on Growth, Morphological and Genetic Stability of <i>Eurycoma longifolia</i> Hairy Root Cultures through ISSR and DAMD Analysis.","authors":"Mahmoud Ali Khalaf Abushattal, Sani Sale, Sreeramanan Subramaniam, Nor Hasnida Hassan, Mohamad Fadhli Mad' Atari","doi":"10.21315/tlsr2025.36.2.9","DOIUrl":"10.21315/tlsr2025.36.2.9","url":null,"abstract":"<p><p>Light quality significantly influences plant growth and development by interacting with photoreceptors, leading to reversible and irreversible outcomes. This study provides novel insights into the species-specific effects of light-emitting diodes (LEDs) on the morphological characteristics and genetic stability of <i>Eurycoma longifolia</i> hairy root cultures (ELHRCs) under different light qualities. LED treatments included white (400 nm-700 nm), blue (440 nm), mint green (530 nm), red (660 nm) and a combination of blue with red (1:1) (440 nm + 660 nm) applied for 8, 10 and 12 weeks. Morphological changes and growth were assessed alongside genetic stability through direct amplification of minisatellite DNA regions (DAMD) and inter-simple sequence repeat (ISSR) markers analysis using 12 primers. The results showed genetic similarity of 90.6% after 8 weeks and 100% after 10 and 12 weeks (DAMD) and 100%, 98.2% and 90.3% after 8, 10 and 12 weeks, respectively (ISSR) under all LED treatments, confirming the genetic stability of the hairy roots. Additionally, the study demonstrated how spectral quality influences hairy roots growth and morphology. The high percentage of genetic similarity highlights LEDs as a promising tool for <i>in vitro</i> culture of ELHRCs. These findings represent the first comprehensive report on the combined effects of LED spectral quality on growth, morphological changes and genetic stability in ELHRCs.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"179-201"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of High-Throughput Screening Assay using 384-Well Plate for Identification of Potent Antioxidants from Malaysian Local Plants Repository and Phytochemical Profile of <i>Tetracera Scandens</i>.","authors":"Amyra Amat Sain, Azimah Amanah, Mohd Hasnan Mohd Noor, Wai Kwan Lau, Olalere Olusegun Abayomi, Zafarina Zainuddin","doi":"10.21315/tlsr2025.36.2.2","DOIUrl":"10.21315/tlsr2025.36.2.2","url":null,"abstract":"<p><p>The increasing prevalence of chronic diseases and oxidative stress-related conditions has led to a growing interest in natural products with potent antioxidant properties. High-throughput screening (HTS) assays offer a promising solution for the rapid evaluation of numerous samples. This study aims to establish a robust HTS assay using the 2-Diphenyl-1-Picrylhydrazyl (DPPH) method to identify natural products with significant antioxidant capabilities, ensuring their safety profiles through cytotoxicity screening and phytochemical profiling. An automated liquid handler with the usage of a 384-well assay plate was employed in optimising and validating the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-HTS assay by calculating the metrics. The HTS metrics include Z-prime (Z') values of 0.72 and 0.63, signal to background (S/B) ratios of 3.54 and 9.02, and coefficient of variation percentages (%C/V) of 4.25 and 6.49 for each primary and secondary screenings, respectively. These values indicated that the HTS assay was excellent. By using optimised HTS-DPPH assay programme, a total of 363 plant extracts were screened and 58 (16%) were found to have potent antioxidant activity (a 'yes' score and EC₅₀ < 50 μg/mL). Out of these 363 extracts, only 80 plants were identified with herbarium and the screening results revealed that <i>Tetracera scandens</i> along with other 11 plants have potent antioxidant activities. <i>T. scandens</i> was the most potent and the methanol extract from its leaves recorded an EC₅₀ value of 13.041± 0.82 μg/mL. However, the aqueous extract of <i>T. scandens</i> leaves (TSLAE) was selected to allow any possibilities of using it for traditional herbal preparation. TSLAE presented an EC₅₀ value of 13.76 ± 4.50 μg/mL in the DPPH assay and was non-toxic towards normal cells with an IC₅₀ value of > 100 μg/mL. Secondary metabolites with promising antioxidant potentials were successfully identified using LC/MS and the library, which were mainly flavonoids (37.5%), phenolics (8.9%) and polyphenols (8.9%). HTS-DPPH is a robust and rapid technique for screening of antioxidative substances in plant extracts. The hit is non-toxic in vitro and rich in secondary metabolites that contribute to antioxidant activity.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"23-57"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> Antiplasmodial and Molecular Docking Studies of Chemical Constituent Isolated from the Bark of <i>Diospyros lanceifolia</i> (Ebenaceae).","authors":"Ibrahim Dankane Bafarawa, Muhammad Solehin Abd Ghani, Arba Pramundita Ramadani, Shofiatul Fuadah, Sista Werdyani, Unang Supratman, Muhammad Bisyrul Hafi Othman, Mohamad Nasir Mohamad Ibrahim, Khalijah Awang, Marc Litaudon, Mohammad Tasyriq Che Omar, Habibah A Wahab, Mohamad Nurul Azmi","doi":"10.21315/tlsr2025.36.2.10","DOIUrl":"10.21315/tlsr2025.36.2.10","url":null,"abstract":"<p><p>The phytochemical investigations of the ethyl acetate bark extract of <i>Diospyros lanceifolia</i> have led to the isolation of eight compounds, namely lupeol (<b>1</b>), betulin (<b>2</b>), β-sitosterol (<b>3</b>), oleic acid (<b>4</b>), α-amyrin acetate (<b>5</b>), glyceryl trilinoleate (<b>6</b>), β-amyrin (<b>7</b>) and shinanolone (<b>8</b>). The structures of all compounds were established using various spectroscopic techniques such as 1D and 2D-NMR, FT-IR and HRESIMS, which were then compared with reported literature for validation. All compounds isolated from this plant were screened for an <i>in vitro</i> study against <i>Plasmodium falciparum</i> FCR3 followed by an <i>in silico</i> molecular docking study with the <i>Pf</i>ATP6 protein. The in vitro results revealed that five compounds exhibited strong to good activity (IC₅₀ < 10 μM). In order of potency, these compounds include <b>5, 3, 6, 1</b> and <b>4</b> with IC₅₀ values of 0.3 ± 0.3 μM, 0.3 ± 0.3 μM, 1.9 ± 2.2 μM, 4.4 ± 7.4 μM and 8.4 ± 4.9 μM, respectively. Compounds <b>5</b> and <b>3</b> showed the strongest activity compared to the control drugs artemisinin and chloroquine, with the IC₅₀ of 0.7 ± 0.3 μM and 10.3 ± 2.9 μM, respectively. The <i>in silico</i> molecular docking simulations showed that all active compounds from the <i>in vitro</i> study displayed good binding affinity to the <i>Pf</i>ATP6 protein binding site, with compounds <b>3, 1</b> and <b>5</b> demonstrating greater binding affinity compared to the other compounds tested, including artemisinin and chloroquine. All compounds exhibited several hydrophobic interaction modes with amino acids of <i>Pf</i>ATP6 residues. Interestingly, all compounds exhibited hydrogen bonding with ASN1039 residue, except compound <b>3</b>. The <i>in silico</i> study of these compounds supports the <i>in vitro</i> antiplasmodial activity findings, suggesting that these compounds are potential lead candidates for the development of new antiplasmodial drugs.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"203-227"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting of Plant-Beneficial <i>Bacillus</i> Species for Growth Promotion and Disease Suppression.","authors":"Ruth Meike Jayanti, Ike Marisna, Jilan Tsani Abdullah, Suryanti, Tri Joko","doi":"10.21315/tlsr2025.36.2.1","DOIUrl":"10.21315/tlsr2025.36.2.1","url":null,"abstract":"<p><p>Plant growth-promoting bacteria (PGPB) have been reported to promote plant growth and protect against plant diseases effectively. PGPB can control plant diseases through direct and indirect mechanisms. The direct mechanism involves the ability to provide nutrients and phytohormones. In contrast, the indirect mechanism refers to the ability to suppress the activity of pathogens through the production of various compounds and metabolites. The purpose of this study is to evaluate the plant health-promoting potential of <i>Bacillus</i> species. Several genetic determinants in 18 isolates of PGPB were investigated via polymerase chain reaction based on the genes <i>fenD, sfp, bamC, ituA, aiiA, ipdC and nifH</i>. Plant-beneficial traits were confirmed through seedling growth tests and <i>in vitro</i> antagonistic assays in the laboratory, followed by a field experiment that used selected <i>Bacillus</i> isolates to improve plant growth and control twisted disease in shallots. Results revealed that two <i>Bacillus</i> isolates, B-27 and RC76, have potential as PGPB. Isolates B-27 and RC76 were identified as <i>Bacillus velezensis</i> and <i>B. tropicus</i>, respectively, based on <i>gyrB</i> sequence analysis. The application of <i>B. velezensis</i> B-27 by spraying resulted in the lowest intensity of twisted disease in shallots. In addition, combined treatment with <i>B. velezensis</i> B-27 and <i>B. tropicus</i> RC76 increased plant height and leaf number.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"1-21"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Significance of Rhizobacteria for Strawberry Cultivation in Tropical Area: A Review.","authors":"Febby Fitriyani, Etty Pratiwi, Masako Akutsu, Reginawanti Hindersah, Syariful Mubarok","doi":"10.21315/tlsr2025.36.2.15","DOIUrl":"10.21315/tlsr2025.36.2.15","url":null,"abstract":"<p><p>In tropical regions, high temperatures and low nitrogen (N) and phosphorus (P) in soil limit plant performance and fruit production. The soil-beneficial microbes, including rhizobacteria, have the potential to overcome the nutrient problems in the soil. Rhizobacteria fix the dinitrogen, solubilise the P and potassium (K), and produce hormones and other metabolites to stimulate plant development and resistance against environmental challenges like inadequate soil fertility, heavy metal concentrations or drought. Bacterial genera that occur for promoting growth is <i>Bacillus</i> sp., <i>Azospirillum</i> sp., <i>Azotobacter</i> sp, <i>Pantoea</i> sp. and <i>Pseudomonas</i> sp. Despite the prominent role of rhizobacteria in agriculture and the economic value of strawberries, the potential use of rhizobacteria as a biofertiliser in strawberry cultivation in tropical areas is rarely discussed and reviewed. The information obtained from publications from 2014-2023 by using the keywords of Plant Growth Promoting Rhizobacteria (PGPR), tropics, biofertiliser, N fixation, P and K solubilisation, P mineralisation, phytohormones and strawberry is organised according to the rhizobacteria, mechanisms by which they boost plant growth, and research location in tropical area. This review focuses on evaluating (i) the mechanism of rhizobacteria to increase plant growth, (ii) the role of rhizobacteria on strawberry growth, yield and quality and (iii) the impact of rhizobacteria on biotic and abiotic stress alleviation.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"317-337"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Shade and Leachate from Invasive <i>Chromolaena odorata</i> (Siam Weed) on Seedling Growth and Development of Native Tree Species in Nepal.","authors":"Sunita Poudel, Ramesh Raj Pant, Mukesh Kumar Chettri, Lal Bahadur Thapa","doi":"10.21315/tlsr2025.36.2.14","DOIUrl":"10.21315/tlsr2025.36.2.14","url":null,"abstract":"<p><p>The weed <i>Chromolaena odorata</i> has negative impacts on invaded ecosystems. Canopy of its aerial parts and allelochemicals released by the weed can suppress the growth and survival of native species. Field assessment of native trees <i>Aegle marmelos</i> and <i>Senegalia catechu</i> evidenced that a declining trend of their seedlings under higher canopy of <i>C. odorata</i>. Experiments carried out in pots revealed the negative effects of the weed's leachate and shade on growth and development of <i>A. marmelos</i>. The leachate increased proline levels in <i>A. marmelos</i> seedlings by ca. 33% in shade and 43% in light. Specific leaf area and secondary roots were decreased significantly under both light and shade conditions by leachate. In the light condition, leachate reduced seedling biomass by ca. 26% and root length by 16%. Shade alone decreased overall seedling growth, including leaf area and biomass with poor root growth and increased specific leaf area. Results showed that <i>A. marmelos</i> is susceptible to shade and <i>C. odorata</i> leachate during its early stage of growth and development. In addition to other factors contributing to the decline of <i>A. marmelos</i> population in nature, the invasion of <i>C. odorata</i> intensifies the challenge. Our study clarifies that the invasion of <i>C. odorata</i> in native habitat has further contributed to the population decline of native species alongside other contributing factors in nature. Hence, there is an urgent necessity to control and manage <i>C. odorata</i> to protect native species. Removal of <i>C. odorata</i> from the invaded site will be beneficial for approaching light for native seedlings as well as preventing the leaching substances into the soil.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"297-316"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Endophytic and Rhizospheric Fungi on The Growth and Specialized Metabolite Production of <i>Phyllanthus niruri</i> L.","authors":"Winda Nawfetrias, Yuda Purwana Roswanjaya, Nur Alfi Saryanah, Sulastri Sulastri, Lukita Devy, Rizkita Rachmi Esyanti, Ahmad Faizal","doi":"10.21315/tlsr2025.36.2.13","DOIUrl":"10.21315/tlsr2025.36.2.13","url":null,"abstract":"<p><p>Medicinal plants are rich sources of specialised metabolites, crucial for various fields like agriculture, forestry, food processing, biofuels and environmental remediation. Microbes, particularly endophytic and rhizospheric fungi, play a significant role in optimising the production and regulation of these compounds. Several research showed these fungi in various plants, but they have not been thoroughly studied in <i>Phyllanthus niruri</i>, especially to promote growth and elicit lignan compounds like phyllanthin in <i>P. niruri</i>. A total of 131 fungi, consist of 57 rhizospheric fungi and 74 endophytic fungi, were successfully isolated from <i>P. niruri</i> in six different lowland areas in West Java. The potency assay results indicated that 106 fungi could produce indole-3-acetic acid (IAA). Six fungi could synthesise cellulase, and one fungus had the capability to solubilise phosphate. Our results showed that <i>Fusarium sp., Colletotrichum gloeosporioides, Colletotrichum tenuissimum, Colletotrichum fructicola, Pseudallescheria boydii, Aspergillus aculeatus, Myrothecium inundatum, Colletotrichum truncatum</i> can synthesise IAA. <i>Fusarium</i> sp. and <i>Myrothecium inundatum</i> could synthesise cellulase and only <i>Aspergillus aculeatus</i> have activity as phosphate solubilisation. Cocultivation of <i>P. niruri</i> and eight endophytic and rhizospheric fungi showed that <i>Fusarium</i> sp., <i>C. gloeosporioides, P. boydii, A. aculeatus</i> and <i>M. inundatum</i> had the potential traits to increase biomass, phyllanthin levels and phyllanthin yield. In general, these fungi have the potency to be elicitors to enhance phyllanthin in <i>P. niruri</i>.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"36 2","pages":"265-295"},"PeriodicalIF":0.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12618096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}