Tropical life sciences research最新文献_第4页

Alleviation of Cadmium Stress in Rice Seedlings Inoculated with Enterobacter tabaci 4M9 (CCB-MBL 5004). 接种了 Enterobacter tabaci 4M9 (CCB-MBL 5004) 的水稻秧苗对镉胁迫的缓解。

IF 1.1

Tropical life sciences research Pub Date : 2024-03-01 Epub Date: 2024-03-30 DOI: 10.21315/tlsr2024.35.1.6

Saidu Abdullahi, Hazzeman Haris, Kamarul Zaman Zarkasi, Amir Hamzah Ghazali

{"title":"Alleviation of Cadmium Stress in Rice Seedlings Inoculated with Enterobacter tabaci 4M9 (CCB-MBL 5004).","authors":"Saidu Abdullahi, Hazzeman Haris, Kamarul Zaman Zarkasi, Amir Hamzah Ghazali","doi":"10.21315/tlsr2024.35.1.6","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.6","url":null,"abstract":"The growth of crop plants is greatly affected by the increased toxicity of metals. Luckily, certain beneficial bacteria can potentially reduce the effects of metal stress and promote the growth of the host plants. Many species of bacteria were reported as heavy metal tolerant and plant growth promoting, with very little or no report available concerning Enterobacter tabaci as heavy metal tolerant plant growth promoting. The present study aimed to evaluate the potential of Cadmium (Cd) tolerant Enterobacter tabaci 4M9 (CCB-MBL 5004) to alleviate heavy metals stress and enhance the growth of rice seedlings grown under Cd stress conditions. Rice seedlings were grown in Yoshida medium supplemented with different concentrations of Cd and inoculated with 4M9. The results showed that the inoculum tested successfully reduced oxidative stress in the seedlings by reducing the electrolyte leakage (EL) and increasing catalase (CAT) and superoxide dismutase (SOD) activities in the inoculated seedlings compared to the control counterparts. The results also revealed a significant increase in plant growth, biomass, and chlorophyll content of inoculated rice seedlings compared to the control. In general, the Cd tolerant E. tabaci 4M9 confers heavy metal alleviation and thereby improves the growth and survival of rice seedlings under Cd stress conditions. Therefore, the findings stated the potential of 4M9 for alleviating heavy metal stress and promoting the development of inoculated rice seedlings if accidentally grown under Cd-contaminated conditions.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"35 1","pages":"107-121"},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of Web-Building Spiders in Gua Kelam, Perlis State Park, Malaysia. 在马来西亚 Perlis 州立公园 Gua Kelam 发现织网蜘蛛。

IF 1.1

Tropical life sciences research Pub Date : 2024-03-01 Epub Date: 2024-03-30 DOI: 10.21315/tlsr2024.35.1.5

Johan Ariff Mohtar, Khadijah Hanim Abdul Rahman, Saktheswaran Nyanasilan, Nurul Ain Harmiza Abdullah, Fadhilah Mohamad

{"title":"Discovery of Web-Building Spiders in Gua Kelam, Perlis State Park, Malaysia.","authors":"Johan Ariff Mohtar, Khadijah Hanim Abdul Rahman, Saktheswaran Nyanasilan, Nurul Ain Harmiza Abdullah, Fadhilah Mohamad","doi":"10.21315/tlsr2024.35.1.5","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.5","url":null,"abstract":"A cave represents a subterranean ecosystem that harbours a myriad of unique, peculiar, and secluded flora and fauna. These biotas have evolved with a wide range of ecological adaptations that allow them to thrive in harsh environments with limited light. Gua Kelam 1 constitutes part of the Gua Kelam limestone caves system in the Nakawan Range of Perlis State Park, Malaysia. Previous observations indicated that it harbours a plethora of spider species; however, their existence is still elusive as speleobiological studies remain unexplored. Herein, we identified the cavernicolous spiders found in the dark zone areas of Gua Kelam 1 through a complementary approach based on morphology and DNA barcoding. From the morphological analysis, we described three web-building spiders of JTKK2 and JTKK3 groups down to the species-level to belong to Nephilengys malabarensis, and Orsinome vethi except for Pholcus sp. from JTKK4 individuals. The molecular analysis of the cytochrome oxidase-I (COI) genes of JTKK2 and JTKK3 individuals showed that they exhibited a high degree similarity with N. malabarensis (98.3%), and O. vethi (100.0%), respectively except for JTKK4 individuals with only 91.4% homology with P. kuhapimuk. Phylogenetic analysis also generated a congruent tree, in which the identified species are well nested within the family Araneidae, Tetragnathidae, and Pholcidae. By this integral approach, the three spiders were determined as N. malabarensis, O. vethi, and Pholcus sp. These spiders are originally epigean in their habitat but uniquely thrive in Gua Kelam 1.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"35 1","pages":"87-106"},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Bacteria Induce Thiamine (Vitamin B1) Production in Oil Palm (Elaeis guineensis). 内生细菌诱导油棕（Elaeis guineensis）产生硫胺素（维生素 B1）。

IF 1.1

Tropical life sciences research Pub Date : 2024-03-01 Epub Date: 2024-03-30 DOI: 10.21315/tlsr2024.35.1.1

Nur Asna Faiqah Johari, Aisamuddin Ardi Zainal Abidin, Nur Farhah Nabihan Ismail, Zetty Norhana Balia Yusof

{"title":"Endophytic Bacteria Induce Thiamine (Vitamin B1) Production in Oil Palm (Elaeis guineensis).","authors":"Nur Asna Faiqah Johari, Aisamuddin Ardi Zainal Abidin, Nur Farhah Nabihan Ismail, Zetty Norhana Balia Yusof","doi":"10.21315/tlsr2024.35.1.1","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.1","url":null,"abstract":"Thiamine or vitamin B1 is a micronutrient that has a crucial function in all living organisms and involved in several biochemical reactions. Concerning the capability of thiamine in inducing plant health, a study was carried out by applying bacterial endophytes (Pseudomonas aeruginosa and Burkholderia cepacia cultures) in four-month-old oil palm seedlings (Elaeis guineensis) via soil drenching technique to evaluate the effect towards thiamine. Spear leaves were sampled day 0 to 14 to analyse the expression of gene coding for the first two enzymes thiamine biosynthesis pathway, THI4 and THIC via qPCR analysis. The gene expression by qPCR showed a significant increase of up to 3-fold while high-performance liquid chromatography (HPLC) analysis for quantification of thiamine and its derivatives accumulated ~ 20-fold in total thiamine when compared to control seedlings. However, concentration of thiamine metabolites was negatively correlated with the expression of THIC and THI4 gene transcripts suggesting post-transcriptional regulation mediated by an RNA regulatory element, a thiamine pyrophosphate (TPP) riboswitch. Our findings demonstrated that the application of bacterial endophytes affected thiamine biosynthesis and enhanced overall thiamine content. This might increase the plant's resistance towards stress and would be useful in oil palm maintenance for maximum yield production.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"35 1","pages":"1-12"},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico EST-SSR Identification and Development through EST Sequences from Metroxylon sagu Rottb. for Genetic Diversity Analysis. 通过用于遗传多样性分析的 Metroxylon sagu Rottb.EST序列，进行默克EST-SSR鉴定和开发。

IF 1.1

Tropical life sciences research Pub Date : 2024-03-01 Epub Date: 2024-03-30 DOI: 10.21315/tlsr2024.35.1.2

Devit Purwoko, Siti Zulaeha, Teuku Tajuddin, Farida Rosana Mira, Maharani Dewi Solikhah, Gemilang Rahmadara, Nurul Fitri Hanifah, Rusmanto

{"title":"In silico EST-SSR Identification and Development through EST Sequences from Metroxylon sagu Rottb. for Genetic Diversity Analysis.","authors":"Devit Purwoko, Siti Zulaeha, Teuku Tajuddin, Farida Rosana Mira, Maharani Dewi Solikhah, Gemilang Rahmadara, Nurul Fitri Hanifah, Rusmanto","doi":"10.21315/tlsr2024.35.1.2","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.2","url":null,"abstract":"Sago plant (Metroxylon sagu Rottb.) is one of the most carbohydrate-producing plants in the world. Microsatellites or simple sequence repeats (SSRs) play an important role in the genome and are used extensively compared to other molecular markers. For the first time, we are exploiting data expressed sequence tags (EST) of sago plants to identify and characterise markers in this species. EST data about sago plants are obtained through the EST database on the National Center for Biotechnology Information (NCBI) website. We obtained data of 458 Kb (412 contig) with a maximum and minimum length of 1,138 and 124 nucleotides, respectively. We successfully identified 820 perfectly patterned SSR using Phobos 3.3.12 software. The type characterisation of EST-SSR was dominated by tri-nucleotides 36% (294), followed by hexa-nucleotides 24% (202), tetra-nucleotides 15% (120), penta-nucleotides 13% (108) and di-nucleotides 12% (96). The most frequency of SSR motifs in each type is AG, AAG and AAAG. Analysis of synteny on the EST sequence with the online application Phytozome found that sequences were distributed on 12 Oryza sativa chromosomes with a likeness percentage between 63% to 100% and e-value between 0 to 0.094. We developed the primer and generated 19 primers. Furthermore, we validated 7 primers that all generated polymorphic alleles. To our knowledge, this report is the first identification and characterisation of EST-SSR for sago species and these markers can be used for genetic diversity analysis, marker assisted selection (MAS), cultivar identification, kinship analysis and genetic mapping analysis.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"35 1","pages":"13-32"},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity, Encounter Rate and Detection of Non-Volant Nocturnal Mammals on Two Malaysian Islands. 马来西亚两座岛屿上的非夜行哺乳动物的多样性、相遇率和检测率。

IF 1.1

Tropical life sciences research Pub Date : 2024-03-01 Epub Date: 2024-03-30 DOI: 10.21315/tlsr2024.35.1.4

Priscillia Miard, Foo Kai Xin, Sapphire Hampshire, Nik Fadzly Nik Rosely, Henry Bernard, Nadine Ruppert

{"title":"Diversity, Encounter Rate and Detection of Non-Volant Nocturnal Mammals on Two Malaysian Islands.","authors":"Priscillia Miard, Foo Kai Xin, Sapphire Hampshire, Nik Fadzly Nik Rosely, Henry Bernard, Nadine Ruppert","doi":"10.21315/tlsr2024.35.1.4","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.4","url":null,"abstract":"Nocturnal mammals constitute a crucial component of tropical faunal diversity, but not much is known about the effects of anthropogenic disturbance on the habitat use and detectability of these species. We investigated which habitat and environmental variables impact the detectability of non-volant nocturnal arboreal mammals across varying habitat types at two tropical islands with different levels of anthropogenic development in Malaysia. We conducted night transect line and point count surveys following pre-existing paths in Penang Island and Langkawi Island between 2019 and 2020. We used a head torch with red filter and a thermal imaging device (FLIR) to enhance animal detection success. We calculated the encounter rates (individual km-1) for each species as a proxy for abundance. Overall, we detected 17 species, but did not find higher species diversity in intact forested environments compared to disturbed areas. Encounter rates of the most observed species were influenced by 'time after sunset' on the highly developed island of Penang, whereas on the rural island of Langkawi, detection was higher in sites with better canopy connectivity. Different species of non-volant nocturnal arboreal mammals use their respective habitats differently and thus, are differently impacted by varying levels of anthropogenic activities. Our results provided baseline data on the diversity, encounter rate, and detectability of these highly elusive species, which can also help to further improve methodologies for the detection of nocturnal wildlife.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"35 1","pages":"49-85"},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agro-Industrial Wastes as Potential Substrates for Rhamnolipid Production by Pseudomonas aeruginosa USM-AR2. 农工业废物作为铜绿假单胞菌 USM-AR2 生产鼠李糖脂的潜在底物。

IF 1.1

Tropical life sciences research Pub Date : 2024-03-01 Epub Date: 2024-03-30 DOI: 10.21315/tlsr2024.35.1.3

Mohd Shafiq Nasir, Ahmad Ramli Mohd Yahya, Nur Asshifa Md Noh

{"title":"Agro-Industrial Wastes as Potential Substrates for Rhamnolipid Production by Pseudomonas aeruginosa USM-AR2.","authors":"Mohd Shafiq Nasir, Ahmad Ramli Mohd Yahya, Nur Asshifa Md Noh","doi":"10.21315/tlsr2024.35.1.3","DOIUrl":"https://doi.org/10.21315/tlsr2024.35.1.3","url":null,"abstract":"Rhamnolipid has gained much attention in various fields owing to its distinctive functional properties compared to conventional chemical surfactants, which are mostly derived from petroleum feedstock. Production cost is one of the main challenges in rhamnolipid production, particularly when using refined substrates. One possible solution is to use agro-industrial wastes as substrates for rhamnolipid production. This is a promising strategy due to their abundance and commercially low value, while simultaneously alleviating an agro-industrial waste management problem in the environment. This study aims to evaluate agro-industrial wastes from local crops as possible low-cost alternative substrates for rhamnolipid production by a local isolate, Pseudomonas aeruginosa USM-AR2. Various liquid wastes, namely sugarcane molasses, rice washing water, overly mature coconut (OMC) water, empty fruit bunch (EFB) steam effluent, palm sludge oil (PSO) and palm oil mill effluent (POME) were screened as the main carbon source supplementing mineral salt medium (MSM) in the fermentation of P. aeruginosa USM-AR2. Batch fermentation was carried out in a shake flask system, agitated at 200 rpm and incubated at room temperature, 27 ± 2°C for 120 h. Among the substrates tested, PSO exhibited the highest biomass at 20.78 g/L and rhamnolipid production at 1.07 g/L. This study has shown the potential of agro-industrial wastes in Malaysia as an alternative resource for rhamnolipid production, transforming them into value added products, while reducing the amount of wastes discharged into the environment.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"35 1","pages":"33-47"},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined Multivariate Statistical Techniques and Water Quality Index (WQI) to Evaluate Spatial Variation in Water Quality. 将多元统计技术与水质指数（WQI）相结合来评估水质的空间变化。

IF 1.4

Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.7

Shaheen Begum, Shahana Firdous, Zainab Naeem, Gul-E-Saba Chaudhry, Shanza Arshad, Fakiha Abid, Sania Zahra, Sehrish Khan, Muhammad Adnan, Yeong Yik Sung, Tengku Sifzizul Tengku Muhammad

{"title":"Combined Multivariate Statistical Techniques and Water Quality Index (WQI) to Evaluate Spatial Variation in Water Quality.","authors":"Shaheen Begum, Shahana Firdous, Zainab Naeem, Gul-E-Saba Chaudhry, Shanza Arshad, Fakiha Abid, Sania Zahra, Sehrish Khan, Muhammad Adnan, Yeong Yik Sung, Tengku Sifzizul Tengku Muhammad","doi":"10.21315/tlsr2023.34.3.7","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.7","url":null,"abstract":"In present study, Water Quality Index (WQI) has been assessed of the Rawal Lake which is a major source of drinking water for people in the Federal Capital, Islamabad, and its adjacent city Rawalpindi in Pakistan. For this, the principal component analysis (PCA) and WQI were applied as an integrated approach to quantitatively explore difference based on spatial variation in 11 water quality parameters of the five major feeding tributaries of the Rawal Lake, Pakistan. The results of temperature in water, total dissolved solids, pH, electrical conductivity, chlorides and sulfates were well within the allowable World Health Organisation's (WHO) limits. However, the heavy metals like cadmium and lead were above permissible limits by the WHO in tributaries of Bari Imam and Rumli. Moreover, this has been proven by the Pearson correlation which suggested strong positive correlation (0.910*) between lead and cadmium. The results of present study were subjected to statistical analysis, i.e., PCA which gave three major factors contributing 96.5% of the total variance. For factor 1, pH, TDS, alkalinity, chlorides, sulfates and zinc have highest factor loading values (>0.60) and presented that these parameters were among the most significant parameters of first factor. As per the WQI results, the water was categorised in two major classes indicating that water of Bari Imam and Rumli is highly contaminated with heavy metals and totally unsuitable for drinking purposes. Based on the results of the present study, it is suggested to make heavy metals consideration as an integrated component in future planning for maintaining water quality of the Rawal Lake and its tributaries.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"34 3","pages":"129-149"},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Environmental Impacts and Development Benefits of Coastal Aquaculture in Three Tropical Countries: Madagascar, Tanzania and Indonesia. 马达加斯加、坦桑尼亚和印度尼西亚三个热带国家沿海水产养殖的环境影响和发展效益比较。

IF 1.4

Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.15

Andreas Kunzmann, Gildas Todinanahary, Flower E Msuya, Yustian Alfiansah

{"title":"Comparative Environmental Impacts and Development Benefits of Coastal Aquaculture in Three Tropical Countries: Madagascar, Tanzania and Indonesia.","authors":"Andreas Kunzmann, Gildas Todinanahary, Flower E Msuya, Yustian Alfiansah","doi":"10.21315/tlsr2023.34.3.15","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.15","url":null,"abstract":"Aquaculture is still in early development in Madagascar and Tanzania, while in Indonesia, aquaculture has a long history. In Madagascar, villagers are farming seaweed and sea cucumbers, as part of small-scale community-based aquaculture (CBA). They followed a contractual model between a private farming company and farmers. Local non-governmental organisations (NGOs) and public institutions in Madagascar jointly strive to reverse the trend of ongoing anthropogenic coastal degradation. In Tanzania, the cultivation of red seaweeds has been established for over 30 years, with declining production attributed to climate change. While shrimp farming still involves, to some extent, clearing of mangroves in Tanzania, seaweed culture has only mild impact on coastal ecosystems. Farming areas provide shelter and habitat for juvenile fish, crabs and other organisms. Therefore, NGOs ask for support to improve culture methods. Various problems and shortcomings in Indonesia have been clearly identified, including issues related to new aquaculture areas, pollutants, emerging diseases, insufficient broodstock and fry supply, as well as a lack of technology and manpower. To address these challenges and ensure the growth of aquaculture production, the government has implemented national policies and established training and broodstock centers throughout the country. In Madagascar, the CBA programme stands out as a success story and can serve as a template for other coastal regions and countries. In Tanzania, the adoption of CBA model for co-culture could be the future. In Indonesia, due to a very long coastlines and complicated legislation, IMTA seems to be particularly suitable, as successfully tested in model regions.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"34 3","pages":"279-302"},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optimising Wastewater Treatment: Acinetobacter sp. IrC1 as a potential multi-resistant bacterium for copper accumulation and dyes decolourisation. 优化废水处理：IrC1不动杆菌是一种潜在的多抗性细菌，可用于铜积累和染料脱色。

IF 1.4

Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.3

Wahyu Irawati, Triwibowo Yuwono, Reinhard Pinontoan, Valentine Lindarto

{"title":"Optimising Wastewater Treatment: Acinetobacter sp. IrC1 as a potential multi-resistant bacterium for copper accumulation and dyes decolourisation.","authors":"Wahyu Irawati, Triwibowo Yuwono, Reinhard Pinontoan, Valentine Lindarto","doi":"10.21315/tlsr2023.34.3.3","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.3","url":null,"abstract":"Improper disposal of waste containing copper and dye is an environmental issue that must be resolved immediately due to its harmful, non-degradable and toxic properties. Bioremediation efficiency can improve by cultivating copper and dye multi-resistant bacteria to remove various pollutant types simultaneously. This study aims at establishing the multi-resistance of Acinetobacter sp. IrC1 to copper and dyes. The effects of copper concentration on growth were determined using a spectrophotometer, while accumulation was analysed using an atomic absorption spectrophotometer. Bacteria-mediated dye decolourisation dyes were observed based on clear zone formation around bacterial colonies, while decolourisation percentage was calculated using a spectrophotometer. Results demonstrate that Acinetobacter sp. IrC1 resisted up to 8 mM CuSO4 and accumulated up to 292.93 mg/g dry weight of copper cells. Acinetobacter sp. IrC1 isolates were also resistant to 500 ppm Methylene Blue, Malachite Green, Congo Red, Mordant Orange, Reactive Black, Direct Yellow, Reactive Orange, Remazol, Wantex Red and Wantex Yellow dye, successfully removing up to 68.35% and 79.50% Methylene Blue and Basic Fuchsine in a medium containing 3 mM CuSO4, respectively. Further investigations are required to analyse the genetic composition of multi-resistant bacteria to optimise the effectiveness of indigenous bacterial isolates as bioremediation agents.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"34 3","pages":"37-56"},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Assessment of The Bioactive Compounds and Anticancer Potential of Citrus medica Leaf Extract. 药用柑橘叶提取物的生物活性化合物和抗癌潜力的体外评估。

IF 1.4

Tropical life sciences research Pub Date : 2023-09-01 Epub Date: 2023-09-30 DOI: 10.21315/tlsr2023.34.3.11

Mikkili Indira, Karlapudi Abraham Peele, Srirama Krupanidhi, Kodali Vidya Prabhakar, K B S Vimala, P Satya Kavya, I Sravya, T C Venkateswarulu

{"title":"In Vitro Assessment of The Bioactive Compounds and Anticancer Potential of Citrus medica Leaf Extract.","authors":"Mikkili Indira, Karlapudi Abraham Peele, Srirama Krupanidhi, Kodali Vidya Prabhakar, K B S Vimala, P Satya Kavya, I Sravya, T C Venkateswarulu","doi":"10.21315/tlsr2023.34.3.11","DOIUrl":"https://doi.org/10.21315/tlsr2023.34.3.11","url":null,"abstract":"Citrus medica is a horticultural crop grown in different parts of the world. The plant leaves have medicinal importance in traditional medicine for the treatment of various diseases. The leaves are an underutilised part of the plant, despite having various bioactive compounds with health benefits, with phytochemical analysis having revealed the presence of flavonoids, fatty acids, alkaloids, terpenoids, glycosides, carbohydrates and phytosterols. The biochemical constituents were identified using Fourier-transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS), which confirmed the presence of terpenoids, alcohols, alkanes, phytosterols and fatty acids. Among these, methyl 8, 11, 14-heptadecatrienoate is a linolenic acid, and α-linolenic acid, trimethylsilyl ester and levulinic acid are the predominant compounds belonging to the omega-3 fatty acid group, which has known health benefits. Further, the antimicrobial activity of C. medica plant leaves were tested against certain food-borne pathogens and showed significant results. The minimum inhibitory concentrations ranged from 6.09 mg/mL to 390 mg/mL for bacterial organisms and 48.75 mg/mL to 390 mg/mL for fungal organisms. The antioxidant activity values were 300 μg/mL and 450 μg/mL by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, respectively. The methanolic extract from the C. medica leaves also showed anticancer activity against MCF7 breast cancer cell lines, with an IC50 value of material for developing a healthy processed food such as nutraceuticals and functional foods.","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":"34 3","pages":"197-215"},"PeriodicalIF":1.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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